PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of nihpaAbout Author manuscriptsSubmit a manuscriptNIH Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
J Neurosci. Author manuscript; available in PMC Apr 7, 2010.
Published in final edited form as:
PMCID: PMC2804479
NIHMSID: NIHMS157890
Autocrine activation of neuronal NMDA receptors by aspartate mediates dopamine- and cAMP-induced CREB-dependent gene transcription
Luis E. F. Almeida,* Peter D. Murray,* H. Ronald Zielke,[open diamond] Clinton D. Roby,* Tami J. Kingsbury,*[nabla] and Bruce K. Krueger*§
* Department of Physiology, University of Maryland School of Medicine, 655 W. Baltimore St., Baltimore, Maryland, 21201
[open diamond] Department of Pediatrics, University of Maryland School of Medicine, 655 W. Baltimore St., Baltimore, Maryland, 21201
§ Department of Psychiatry, University of Maryland School of Medicine, 655 W. Baltimore St., Baltimore, Maryland, 21201
[nabla] Program in Oncology, Marlene and Stewart Greenebaum Cancer Center, University of Maryland School of Medicine, 655 W. Baltimore St., Baltimore, Maryland, 21201
Program in Neuroscience, University of Maryland Baltimore, 655 W. Baltimore St., Baltimore, Maryland, 21201
Corresponding author: B. K. Krueger, Dept. of Physiology, University of Maryland School of Medicine, 655 W. Baltimore St., Baltimore, MD 21201 USA. bkrueger/at/umaryland.edu, phone: 410 706 5065, fax: 410 706 8341
Cyclic AMP can stimulate the transcription of many activity-dependent genes via activation of the transcription factor, CREB. However, in mouse cortical neuron cultures, prior to synaptogenesis, neither cAMP nor dopamine, which acts via cAMP, stimulated CREB-dependent gene transcription when NR2B-containing NMDA receptors (NMDARs) were blocked. Stimulation of transcription by cAMP was potentiated by inhibitors of excitatory amino acid uptake, suggesting a role for extracellular glutamate or aspartate in cAMP-induced transcription. Aspartate was identified as the extracellular messenger: enzymatic scavenging of L-aspartate, but not glutamate, blocked stimulation of CREB-dependent gene transcription by cAMP; moreover, cAMP induced aspartate but not glutamate release. Taken together, these results suggest that cAMP acts via an autocrine or paracrine pathway to release aspartate, which activates NR2B-containing NMDARs, leading to Ca2+ entry and activation of transcription. This cAMP/aspartate/NMDAR signaling pathway may mediate the effects of transmitters such as dopamine on axon growth and synaptogenesis in developing neurons or on synaptic plasticity in mature neural networks.
Keywords: aspartate, astrocyte, Ca2+, cyclic AMP, CREB, D1 receptor, dopamine, forskolin, neuron, NMDA receptor, NR2B subunit, gene transcription
Cyclic AMP (cAMP) is a ubiquitous intracellular second messenger that regulates a wide range of cellular processes in the nervous system throughout the vertebrate lifespan, including synaptogenesis, growth of axons, dendrites and dendritic spines, and persistent changes in synaptic plasticity such as long-term potentiation (LTP) (Nguyen and Kandel, 1996; Boulanger and Poo, 1999; Ma et al., 1999; Cai et al., 2001; Kao et al., 2002; Nguyen and Woo, 2003; Tominaga-Yoshino et al., 2002; Otmakhov et al., 2004; Ji et al., 2005; Wozny et al., 2008). Neuronal levels of cAMP are regulated by G-protein-coupled receptors linked to adenylyl cyclase (AC). In the cortex, hippocampus and striatum, the neurotransmitter, dopamine (DA), activates AC via D1/D5 DA receptors to regulate neuronal growth and differentiation during development (Schmidt et al., 1998; Jones et al., 2000) and synaptic plasticity and cognitive function in the mature brain (Huang and Kandel, 1995; Otmakhova and Lisman, 1998; Bach et al., 1999; Huang et al., 2004; Shen et al., 2008).
Cyclic AMP activates cAMP-dependent protein kinase (PKA), which can directly modulate effector proteins by phosphorylation at PKA-specific serine or threonine residues or can alter the levels of the effector proteins themselves by modulating gene expression. Promoters containing cAMP/Ca2+-response elements (CREs) are regulated by the transcription factor, CRE-binding protein (CREB) (Mayr and Montminy, 2001; Impey et al., 2004), which can be activated by extracellular signals that increase the intracellular levels of cAMP or Ca2+ (West et al., 2002; Lonze and Ginty, 2002; Conkright et al., 2003). Cyclic AMP-dependent regulation of transcription via CREB has been implicated in neurogenesis, cell survival, synaptic transmission and cognitive function in the normal nervous system and may be defective in developmental, psychiatric and neurodegenerative disorders (Barco et al., 2002; Lonze and Ginty, 2002; Nguyen and Woo, 2003; Carlezon et al., 2003).
CREB activation requires phosphorylation of serine-133, which can be mediated by PKA, the mitogen-activated protein kinase (MAPK) pathway, or Ca2+/calmodulin-dependent kinases (CaMKs), depending on the activating signal and cell type (Gonzalez and Montminy, 1989; Sheng et al., 1991; Dash et al., 1991). Although CREB phosphorylation is necessary, it may not be sufficient to initiate gene transcription. In many cells, an additional step, e.g., activation of a transcriptional co-activator such as CREB binding protein (CBP/p300; TOX3) (Arias et al., 1994; Brindle et al., 1995; Yuan et al., 2009), is necessary for the assembly of an active RNA polymerase complex and initiation of transcription (Cardinaux et al., 2000; Vo and Goodman, 2001; Xu et al., 2007; Hardingham et al., 1999). Whether activation of both CREB and co-activator is mediated by cAMP- or Ca2+-dependent kinases may depend on cell-specific kinase expression patterns (Matthews et al., 1994; Impey et al., 2002).
Here, we report that, in immature neurons from embryonic mouse cortex, prior to synaptogenesis, DA and cAMP do not stimulate CREB-dependent gene transcription when NMDARs are blocked. Our results suggest that cAMP stimulates CREB-dependent gene transcription via a two-step process by inducing release of an excitatory amino acid, identified here as aspartate, which then activates NMDARs, leading to Ca2+ entry and gene transcription.
Cell cultures
Cultured neurons from embryonic (E15.5) C57Bl6 mouse cortex were maintained in 24-well plates coated with polyornithine and laminin at a density of 3 × 105 cells/well. Culture medium was B27-supplemented Neurobasal (Invitrogen, Carlsbad, CA) containing 1 mM glutamine, penicillin and streptomycin. Cells were grown in 0.5 ml of medium per well and the medium was not changed. All experiments were conducted at 4 –5 div. Although the cultured cells at this age are postmitotic neurons, which have elaborated extensive processes and express neuron-specific immunohistochemical markers, synaptogenesis has not yet begun (c.f., Hardingham and Bading, 2002; Han and Stevens, 2009). Cortical astrocyte cultures from E15.5 C57Bl6 mouse embryos were maintained in poly-L-lysine-coated T75 flasks in DMEM/F12 medium (1/1) supplemented with 10% heat-inactivated fetal bovine serum (Atlanta Biologicals, Lawrenceville, GA) and penicillin and streptomycin. Prior to transfection, astrocytes were replated into 24-well plates (5 × 105 cells/well; 0.5 ml medium/well).
Transfection, plasmids and luciferase reporter assays
Cortical neurons were transfected on 3 div by the Ca2+-phosphate method (Xia et al., 1996). Astrocytes were transfected 1– 2 days after replating using Lipofectamine 2000 (Invitrogen) diluted 1:33 in Opti-MEM without penicillin/streptomycin. The 4xCRE reporter consisted of four CREs inserted in pCIC-CK vector (Stratagene, La Jolla, CA) upstream of the luciferase reporter gene. In control experiments, empty pCIC-CK plasmid was not stimulated by any of the agonists used in this study (not shown; and see Kingsbury et al., 2003). Co-transfection with constitutively active TK Renilla plasmid (Dual-Luciferase Reporter Assay System, Promega, Madison, WI) served as a control for variability in transfection efficiency and cell density. Experiments were conducted 24 hr after transfection. Luciferase activity was measured with a Centro LB 960 microplate luminometer (Berthold Technologies, Oak Ridge, TN). The ratio of 4xCRE luciferase and Renilla activities was computed for each sample and the mean of 3 determinations was expressed as a fraction of unstimulated activity. The result for each experimental condition is reported as mean ± sem (n = 3 –5), where n is the number of independent experiments. Statistical analyses were conducted with ANOVA followed by Tukey test using InStat (GraphPad, San Diego, CA).
cAMP assay
Intracellular cAMP levels were measured by ELISA (Assay Designs, Ann Arbor, MI); cAMP levels are reported as pmoles/106 cells.
Quantitative real-time PCR (Q-PCR)
Neurons were cultured for 3 –4 days in 35mm culture dishes at a density of 1.2 × 106 cells/dish and then stimulated for 1, 3 or 24 hours. Total RNA was purified with Rneasy Mini Kit (Qiagen) including the on-column DNA digestion step. This procedure regularly gave high purity RNA (260/280 nm absorbance ratio ~ 2.0). cDNA was reverse-transcribed (Transcriptor Kit, Roche) using random primers. Reactions were run in a LightCycler 480 (Roche) using SYBR Green I Master Mix (Roche) with the following settings: pre-incubation (5 min @ 95°C); amplification, 45 cycles (10 sec @ 95°C; 15 sec @ 58°C; 1 min @ 72°C); and a melting curve (5 sec @ 95°C followed by 1 min ramp from 65°C to 97°C). All samples generated single melting curve peaks. Primers were used at 0.5 μM concentration and had the following sequences. GAPDH: 5-TGATGACATCAAGAAGGTGGTGAAG-3 (forward) and 5-TCCTTGGAGGCCATGTAGGCCAT-3 (reverse), c-fos: 5-AAGTTGGCACTAGAGACGGACAGA-3 (forward) and 5-ACTTCGACCATGATGTTCTCGGGT-3 (reverse), BDNF-exon I: 5-GGACAGCAAAGCCACAATGTTCCA-3 (forward) and 5-TTGCCTTGTCCGTGGACGTTTACT-3 (reverse) and BDNF-exon IV: 5-ACCAGGTGAGAAGAGTGATGACCA-3 (forward) and 5-GTTGCCTTGTCCGTGGACGTTTA-3 (reverse).
Each sample was run in duplicate and a 3-point (400, 200 and 100 ng) cDNA dilution curve was constructed. The reaction efficiency was calculated for each treatment condition. RNA for each target gene was normalized to GAPDH according to the following formula (Pfaffl, 2001):
equation M1
where Etarget and EGAPDH are the reaction efficiencies for the target and GAPDH PCR reaction respectively; CP is the crossing threshold for each reaction; and control and sample refers to untreated or treated samples, respectively. This equation takes into account the actual measured reaction efficiency for each treatment condition, rather than assuming a theoretical efficiency of 2.0.
Western blot
Neurons grown in polyornithine/laminin-coated 35 mm plastic dishes were stimulated for 3 hours, washed in ice-cold PBS and lysed with Ripa Buffer (Sigma-Aldrich) supplemented with protease (Roche Applied Science, Indianapolis, IN) and phosphatase (Pierce, Rockford, IL) inhibitors. Samples were run in duplicate on pre-cast 4–12% Bis-Tris gels (Invitrogen) and transferred to PVDF membranes. Blots were blocked for 1 hour with tris-buffered saline containing 0.01% Tween-20, 5% powered milk and 5% bovine albumin (Sigma-Aldrich). Membranes were incubated overnight at 4°C with antibody to phospho-S133-CREB (Cell Signaling, Danvers, MA) diluted 1:1000 and incubated with secondary horse radish peroxidase-linked antibodies (GE Healthcare, Piscataway, NJ) at room temperature for 1 hr. Bands were visualized by enhanced chemiluminescence (Millipore, Billerica, MA) and images were acquired and analyzed on an Image Station 2000R (Kodak-Carestream Health, Rochester, NY). Uniform loading was verified by stripping and reprobing with antibodies to total CREB (1:1000; Cell Signaling).
Glutamate and aspartate scavenging systems
To degrade glutamate in the extracellular space, cultures were incubated with 5 units/ml glutamate-pyruvate transaminase (GPT) and 5 mM pyruvate for 20 min prior to stimulation with forskolin. To degrade both L-aspartate and glutamate, cultures were incubated with 5 units/ml glutamate-pyruvate transaminase (GPT), 10 mM pyruvate, 5 units/ml glutamate-oxaloacetate transaminase (GOT) and 3 mM α-ketoglutarate for 20 min prior to stimulation with forskolin. Omission of GPT and GOT served as controls.
Excitatory amino acid analysis
At 4 div, medium (500 μl) from neurons in 24 well plates was removed and replaced by 200 μl of fresh medium; cells were stimulated by addition of forskolin 4 hrs later. HPLC was used to quantify aspartate and glutamate in 250 μl of the combined culture medium from two duplicate wells. Samples were acidified with 0.14 M perchloric acid and neutralized with 0.25 M potassium bicarbonate. Samples were reacted with 2 volumes of o-phthaldehyde (OPA) for 2 minutes before injection on to a C18 reverse phase column (Beckman-Coulter, Fullerton, CA). Derivatized amino acids were detected by fluorescence. The elution was carried out at 0.7 ml/min with a linear gradient mixing 7.5% methanol prepared in 20 mM sodium acetate, pH 7 (solution A) with 30% methanol and 30% acetonitrile prepared in water (solution B). Over the range encompassing aspartate, the gradient ran for 15 min from 97% A and 3% B to 85% A and 15%B. The column was stripped with 100% B following the elution of aspartate and glutamate. The positions of aspartate and glutamate on the chromatogram were identified by running standards and confirmed by spiking experimental samples with these amino acids.
Drugs and reagents
Forskolin, 8-Br-cAMP, NMDA, DL-AP5, ifenprodil, SKF38393, SCH23390, pyruvate, glutamate-pyruvate transaminase (from porcine heart), glutamate-oxaloacetate transaminase type I (from porcine heart), L-glutamate, L-aspartate, OPA and α-ketoglutarate were obtained from Sigma-Aldrich (St. Louis, MO); H89, dideoxy-forskolin, and Bapta-AM from EMD-Calbiochem (San Diego, CA); Bapta-free acid from Invitrogen (Carlsbad, CA); TBOA and trans-PDC from Tocris-Cookson (Ellisville, MO) and EGTA-AM from AnaSpec (San Jose, CA). Antagonists were added 30 minutes before stimulation except for Bapta-AM and EGTA-AM which were added 60 before stimulation.
Stimulation of CREB-dependent transcription by cAMP requires Ca2+
Neuron cultures from E15.5 mouse cortex were transfected at 3 days in vitro (3 div) with a luciferase reporter construct driven by 4 tandem CREs (4xCRE). The next day, the cells were incubated with the AC activator, forskolin, and harvested after 5 hours for measurement of luciferase activity. A maximally-effective concentration of forskolin (10 μM) induced a 16-fold stimulation of 4xCRE luciferase reporter activity (Fig. 1a), whereas the inactive derivative, dideoxy-forskolin, had no effect, even at 30 μM (Supplemental Fig. S1). Stimulation by cAMP was found to require Ca2+. Preincubation of the cultures with either 10 μM 1,2-bis(o-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid-acetoxymethyl ester (Bapta-AM) to chelate intracellular Ca2+, or 3 mM Bapta-free acid (FA) to chelate extracellular Ca2+, completely inhibited 4xCRE stimulation by forskolin (Fig. 1a). Stimulation by forskolin was also inhibited by the cAMP-dependent protein kinase (PKA) antagonist, H89 (10 μM; Supplemental Fig. S1). The membrane-permeant cAMP analog, 8-Br-cAMP, also stimulated 4xCRE luciferase expression and, as with forskolin, stimulation was blocked by chelation of intracellular (Bapta-AM) or extracellular (Bapta-FA) Ca2+ (Fig. 1a). Since 8-Br-cAMP acts independently of adenylyl cyclase, Ca2+ is required for a signaling step downstream from cAMP.
Fig. 1
Fig. 1
(a) Induction of CREB-dependent gene expression by cAMP requires Ca2+. Stimulation of 4xCRE luciferase activity by forskolin (10 μM) or 8-Br-cAMP (3 mM) was abolished by adding 3 mM Bapta-free acid (Bapta-FA) or by pre-treating with Bapta-AM (10 (more ...)
Ca2+ is not required for stimulation of cAMP production by forskolin
The Ca2+-dependence of both 8-Br-cAMP- and forskolin-induced CREB-dependent gene transcription suggests that forskolin is not activating a Ca2+-dependent AC. In order to determine if Ca2+ is required for forskolin to stimulate cAMP production, we examined the effect of a Ca2+ chelator on forskolin-induced cAMP production in neuron cultures. A maximally-effective concentration of forskolin (10 μM) increased the level of cAMP by 12-fold as measured by ELISA (Fig. 1b). Despite the Ca2+ requirement for cAMP to stimulate 4xCRE expression (Fig. 1a), the forskolin-induced rise in cAMP was unaffected by preincubation with 10 μM Bapta-AM, indicating that forskolin is not activating a Ca2+-dependent AC.
Stimulation of CREB-dependent transcription by forskolin requires an increase in Ca2+ within a microdomain
We did not observe an increase in intracellular neuronal Ca2+ in response to forskolin as measured by Ca2+ imaging (data not shown), raising the possibility that spatially-restricted rather than global Ca2+ increases mediate the effects of forskolin on CREB-dependent gene transcription. To investigate this possibility, we compared the effects of Bapta-AM and EGTA-AM on cAMP-induced CREB-dependent transcription. The on-rate for Ca2+ binding to Bapta is fast compared to the rate of intracellular Ca2+ diffusion and intracellular Bapta quickly buffers increases in intracellular Ca2+. In contrast, the on-rate for Ca2+ binding to EGTA is slow. Consequently, unlike Bapta, EGTA will not buffer Ca2+ fast enough to prevent activation of Ca2+-dependent processes within the microdomain in the vicinity of Ca2+ entry (Deisseroth et al., 1996; Naraghi and Neher, 1997; Hardingham et al., 2001; Augustine et al., 2003). When EGTA-AM was substituted for Bapta-AM, stimulation of transcription by forskolin was only reduced by about 30% in contrast to 95% inhibition by Bapta (Fig. 2), suggesting that increased Ca2+ within such microdomains mediates the stimulation of CREB-dependent gene transcription by cAMP.
Fig. 2
Fig. 2
Stimulation of CREB-dependent transcription by forskolin requires an increase in [Ca2+] within a microdomain. One day after transfecting with 4xCRE reporter plasmid, cultures were preincubated with Bapta-AM or EGTA-AM (10 μM) for 1 hr and then (more ...)
Stimulation of transcription by cAMP requires activation of both NR2B-containing N-methyl-D-aspartate (NMDA) receptors and L-type, voltage-gated Ca2+ channels
Since the results in Figure 1a suggested that 4xCRE activation by cAMP requires Ca2+ influx, we examined the effects of inhibitors of the two principal Ca2+ entry pathways in neurons, the NMDA class of ionotropic glutamate receptors (NMDARs) and voltage-gated Ca2+ channels (Cavs). AP5, an antagonist of all subtypes of NMDARs; ifenprodil, a selective inhibitor of NMDARs containing NR2B subunits; as well as nifedipine and verapamil, inhibitors of L-type Cavs, each blocked cAMP-stimulated CRE-luciferase expression by at least 90% (Fig. 3a,b). NMDA, which directly activates NMDARs, also stimulated 4xCRE luciferase activity; this stimulation was blocked by AP5 and ifenprodil, but not by nifedipine (Fig. 3c). Thus, while Ca2+ entering through both NMDARs and Cavs is necessary for cAMP to stimulate 4xCRE transcription (Fig. 3a,b), 4xCRE transcription induced by strong stimulation of NMDARs (Fig. 3c) does not require Cavs.
Fig. 3
Fig. 3
Stimulation of CREB-dependent gene transcription requires activation of NR2B-containing NMDARs and L-type Cavs. Induction of 4xCRE transcription by 10 μM forskolin (a) or 3 mM 8-Br-cAMP (b) was blocked by the NMDAR antagonists AP5 (100 μM) (more ...)
cAMP-induced CREB phosphorylation is not sufficient for transcription
Activation of CRE-dependent gene expression by either cAMP or Ca2+ requires phosphorylation of CREB at serine-133 (Dash et al., 1991; Lonze and Ginty, 2002). Western blot analysis with antibodies specific for phosphorylated CREB serine-133 revealed that forskolin stimulation of CREB phosphorylation was not affected by Bapta-AM or ifenprodil (Fig. 4), both of which blocked forskolin-stimulated 4xCRE transcription (Figs. 1a and and3a).3a). Forskolin-induced CREB phosphorylation was inhibited by the PKA antagonist, H89 (data not shown). Thus, unlike CREB-dependent gene transcription, stimulation of CREB phosphorylation by cAMP is Ca2+ independent and does not require NMDARs. These data suggest that an additional, Ca2+-dependent step, mediated by NMDAR activation, is required for cAMP to stimulate 4xCRE transcription.
Fig. 4
Fig. 4
Phosphorylation of CREB at serine-133 is not sufficient for 4xCRE transcription. (a) Immunoblots were probed for phospho-CREB (top) and then stripped and re-probed for total CREB (bottom) to control for sample variability. Forskolin (F, 10 μM) (more ...)
DA-induced CREB-dependent gene transcription is mediated by NMDARs
DA is a major endogenous neurotransmitter in the cortex as well as in the hippocampus and striatum (Goldman-Rakic et al., 2000). DA acting at the D1/D5 class of DA receptors, which are positively coupled to adenylyl cyclase, stimulates cAMP production. Application of 100 μM DA to cortical neurons stimulated CREB-dependent gene transcription and this effect was potentiated by the cyclic nucleotide phosphodiesterase inhibitor, rolipram (Fig. 5a). Isoproterenol, an agonist of β-adrenergic receptors, which are also positively linked to AC in some neurons, did not stimulate CREB-dependent gene transcription in either the absence or presence of rolipram (Fig. 5a). Stimulation by DA+rolipram was fully blocked by Bapta-AM (10 μM), AP5 (100 μM), ifenprodil (10 μM), verapamil (100 μM), as well as by the D1 DA receptor antagonist, SCH23390 (30 μM) (Fig. 5b). Thus, like forskolin and 8-Br-cAMP (Fig. 3a,b), the endogenous transmitter, DA, which acts via cAMP, does not activate CREB-dependent gene transcription when NR2B-containing NMDARs or L-type Cavs are blocked.
Fig. 5
Fig. 5
DA activates CREB-dependent gene transcription. (a) Cortical neurons transfected with 4xCRE luciferase were stimulated at 4 div with 100 μM DA or the β-adrenergic agonist, isoproterenol, in the absence or presence of 0.1 μM rolipram (more ...)
Stimulation of endogenous BDNF and c-fos transcription by cAMP and DA agonists is also mediated by NR2B-containing NMDARs
To determine if cAMP activation of endogenous CREB-dependent gene transcription also requires NMDARs, we examined the effect of forskolin and the D1/D5 DA receptor agonist, SKF38393 (Johnson et al., 1992), on levels of mRNAs encoding the endogenous CRE-containing genes, c-fos and BDNF (exons I and IV). Ca2+- and cAMP-dependent BDNF transcription is mediated by CREs in exons I and IV (West et al., 2002; Tabuchi et al., 2002). We found that, while forskolin strongly stimulated c-fos mRNA expression within 1 hr and BDNF exon I expression was maximal by 3 hrs, exon IV expression was not substantially increased until 24 hr (Supplemental Figure S2). At 24 hr, stimulation of mRNA encoding c-fos and both BDNF isoforms by forskolin and SKF38393 was inhibited by ifenprodil (Fig. 6). Thus, our finding that stimulation of CRE-dependent gene transcription by DA via cAMP requires activation of NR2B-containing NMDARs is not limited to the 4xCRE reporter and may reflect a common regulatory mechanism of CREB-dependent genes in these neurons.
Fig. 6
Fig. 6
Stimulation mRNA levels of CRE-containing genes by cAMP or D1 DA receptor agonist is mediated by NR2B-containing NMDARs. Cortical neuron cultures were incubated in the absence or presence of forskolin (10 μM), the D1 agonist, SKF38393 (100 μM) (more ...)
An excitatory amino acid (EAA) mediates stimulation of CREB-dependent transcription by cAMP
The block of cAMP-induced, CREB-dependent gene transcription by AP5 and ifenprodil led us to hypothesize that cAMP induces an increase in the extracellular level of an activator of the NMDAR, viz., an EAA secreted by the neurons themselves. Stimulation of neurotransmitter release by cAMP is consistent with several reports in the literature (Chavez-Noriega and Stevens, 1994; Trudeau et al., 1996; Lu et al., 2004; Seino and Shibasaki, 2005; Woodhall et al., 2007). In order to test this hypothesis, we preincubated the neurons with the EAA uptake inhibitor, DL-threo-β-benzyloxyaspartate (TBOA), which would be predicted to potentiate the effects of cAMP. 10 μM TBOA alone had a negligible effect on 4xCRE transcription but potentiated the effects of forskolin and 8-Br-cAMP (Fig. 7). Similar potentiation of the effects of forskolin was obtained with a structurally different EAA uptake inhibitor, L-trans-pyrrolidine-2,4-dicarboxylate (trans-PDC; Supplemental Fig. S3). These results suggested that extracellular glutamate or aspartate mediates the stimulation of CREB-dependent gene transcription by cAMP.
Fig. 7
Fig. 7
EAA uptake inhibition enhances stimulation of CREB-dependent gene transcription by cAMP. The EAA uptake inhibitor, TBOA (10 μM), did not, by itself, stimulate 4xCRE transcription, but potentiated the response induced by forskolin (1 μM) (more ...)
Aspartate mediates stimulation of CREB-dependent transcription by cAMP
If glutamate were the mediator of cAMP-induced CREB-dependent gene transcription, the response should be blocked by an enzymatic glutamate scavenging system (glutamate-pyruvate transaminase, GPT; c.f., O’Brien and Fischback, 1986; Matthews et al., 2003). In control experiments, the combination of pyruvate plus GPT reduced added glutamate to undetectable levels and completely inhibited stimulation of 4xCRE by added glutamate, demonstrating the efficacy of this scavenging system (Supplemental Fig. S4). However, this glutamate scavenger system had no effect on stimulation by forskolin (Fig. 8a). This raised the possibility that aspartate (Fleck et al., 1993; Bradford and Nadler, 2004) rather than glutamate mediates the effects of cAMP in these neurons. To investigate the possible role of aspartate in mediating the stimulation of CRE-dependent transcription by cAMP, we developed a novel aspartate+glutamate scavenging system by adding α-ketoglutarate (α-keto) and glutamate oxaloacetate transaminase (GOT) to the glutamate scavenging system. GOT converts α-keto and L- (but not D-) aspartate to glutamate and oxaloacetate. Thus, both glutamate and L-aspartate are degraded in the presence of GOT, α-keto, GPT and pyruvate. This combination scavenging system completely inhibited stimulation of CREB-dependent gene transcription by forskolin (Fig. 8b), in contrast to the glutamate-only scavenging system, which had no effect (Fig. 8a). Thus, extracellular L-aspartate, but not glutamate, mediates the effect of cAMP on CREB-dependent gene transcription. Because aspartate specifically activates NMDARs, whereas glutamate activates mGluRs and AMPARs as well as NMDARs (Curras and Dingeldine, 1992; Frauli et al., 2006), increased cAMP will initiate selective activation of NMDARs on the target cells.
Fig. 8
Fig. 8
L-aspartate+glutamate scavenger inhibits cAMP-induced 4xCRE transcription but glutamate scavenger does not. (a) Addition of a glutamate-only scavenging system (10 mM pyruvate plus 5 U/ml GPT) did not affect forskolin-induced 4xCRE transcription. (b) Addition (more ...)
cAMP-induces release of aspartate in cortical neuron cultures
The inhibition of cAMP-induced CREB-dependent gene transcription by the aspartate+glutamate scavenger system suggested that cAMP induces release of aspartate. Using high-resolution HPLC, we directly demonstrated that treatment with forskolin for 30 min induced a significant increase in aspartate levels in the medium (Fig. 9). In contrast, glutamate levels in the medium were unchanged in the same cultures. This finding also suggests that aspartate and glutamate release are independently regulated and that aspartate is not simply co-released with glutamate. It should be noted that the 50% increase in aspartate measured after dilution into the bulk medium is likely to be an underestimate of the local increase in aspartate levels in the vicinity of the NMDARs following stimulation by forskolin.
Fig. 9
Fig. 9
cAMP induces aspartate release from cortical neurons. Four hours after medium replacement, cultures were treated with 100 μM forskolin or vehicle (DMSO) for 30 min. 250 μl of the combined medium from duplicate cultures was removed and (more ...)
Transcriptional response to cAMP is mediated by aspartate and activation of NMDARs
As summarized in Figure 10, we postulate that stimulation of CREB-dependent transcription by cAMP occurs in two sequential steps, viz., cAMP induces release of aspartate (Fig. 10, left, dashed arrows), followed by activation of NR2B-containing NMDARs by the aspartate released in response to cAMP. We refer to this as the cAMP/aspartate/NMDAR signaling pathway. All of the experiments reported here were conducted in neuron cultures at 4–5 DIV, prior to the onset of synaptogenesis. Since aspartate release (Fig. 9) and activation of NMDARs (Figs. 3, ,5b)5b) are not synaptic events, we refer to this as an autocrine mechanism (or a paracrine mechanism if the aspartate acts on neighboring neurons).
Fig. 10
Fig. 10
Model illustrating the signaling pathways mediating stimulation of CREB-dependent gene transcription by DA and cAMP in mouse cortical neurons prior to synaptogenesis. Like forskolin, activation of the D1/D5 class of DA receptors (D1/5-AC), which are positively (more ...)
Our hypothesis is based on several lines of evidence. First, the effects of cAMP and DA on both 4xCRE (Fig. 3a,b; Fig. 5) and c-fos and BDNF mRNA expression (Fig. 6) are blocked by NMDAR antagonists. Second, two structurally-distinct inhibitors of neuronal EAA uptake, TBOA (Fig. 7) and trans-PDC (Supplemental Fig. S3), potentiated the stimulation of gene transcription by cAMP. Third, the aspartate+glutamate-, but not the glutamate-only-, scavenging system abolished stimulation of CREB-dependent gene transcription by forskolin (Fig. 8); the aspartate-scavenging enzyme, GOT, degrades L- but not D-aspartate demonstrating that L-aspartate is the active extracellular signal in this signaling pathway. Finally, forskolin was found to induce release of aspartate but not glutamate (Fig. 9). Taken together, these results lead to the conclusion that cAMP-induced release of aspartate and the resulting activation of NR2B-containing NMDARs mediate the effects of cAMP. Other studies have reported that cAMP-induced phosphorylation of NMDARs mediated by PKA enhances permeability of the NMDARs to Ca2+ (Dudman et al., 2003; Skeberdis et al., 2006). Our results are not incompatible with this mechanism, which may act in parallel with the autocrine pathway described here to potentiate the effects of cAMP and DA on CREB-dependent transcription.
Figure 10 depicts cAMP-induced aspartate release from neurons acting by an autocrine (or paracrine) mechanism; other mechanisms were also considered. For example, cAMP-induced release of aspartate from a few contaminating astrocytes in the neuron cultures might have contributed to our results. Astrocytes have been reported to release glutamate which activates nearby neurons (Parpura and Haydon, 2000), but only D-aspartate has been reported to be released from astrocytes (Rutledge and Kimmelberg, 1996). Moreover, astrocytes, themselves, are not wholly responsible for our results because cAMP-stimulated CREB-dependent gene transcription in purified astrocyte cultures is insensitive to NMDAR antagonists (Supplemental Fig. S5). Alternatively, cAMP might specifically inhibit aspartate uptake; however, such an effect of cAMP has not previously been reported. Moreover, aspartate and glutamate are accumulated by the same membrane transporters and forskolin increases aspartate but not glutamate in the medium (Fig. 9). This result argues against inhibition of uptake by cAMP.
We propose that the aspartate-stimulated influx of Ca2+ through NMDARs (Fig. 10, right, solid arrows) is necessary for cAMP to activate gene transcription, even though cAMP can induce CREB phosphorylation in both the presence and absence of NMDAR antagonists (Fig. 4; Fig. 10, dotted arrow). Since stimulation of CREB-dependent gene transcription requires activation of both CREB and a co-activator, Ca2+ entering through NMDARs appears to be required for co-activator activation (Impey et al., 2002).
Ca2+ microdomains in the cAMP/aspartate/NMDAR signaling pathway
Our results indicate that Ca2+ entering through NMDARs is a necessary effector for cAMP-stimulated transcription (Fig. 3a,b; Fig. 10, right, solid arrows). However, we did not observe a global increase in neuronal cytoplasmic Ca2+ in response to forskolin as measured by Ca2+ imaging (data not shown). Unlike Bapta-AM, EGTA-AM had only a small inhibitory effect on cAMP-induced CREB-dependent gene transcription (Fig. 2), suggesting that the relevant increases in [Ca2+] occur within distinct microdomains near the site of Ca2+ entry (Deisseroth et al., 1996; Naraghi and Neher, 1997; Hardingham et al., 2001; Augustine et al., 2003) rather than throughout the cytoplasm.
Role of Ca2+ channels in the cAMP/aspartate/NMDAR signaling pathway
L-type Cav antagonists blocked stimulation of CREB-dependent gene transcription by cAMP (Fig. 3) and D1 DA receptor activation (Fig. 5b), consistent with reports that Cavs are necessary for glutamate-induced c-fos expression in striatal neurons (Rajadhyaksha et al., 1999) and forskolin-induced proenkephalin gene expression in rat striatal neurons (Konradi et al., 2003). However, block of Cavs did not affect forskolin-induced aspartate release (Fig. S6a), suggesting that the Ca2+ entry through Cavs required for CREB-dependent gene transcription is not necessary for forskolin-induced aspartate release. In addition, block of Cavs did not affect stimulation of 4xCRE expression by submaximal concentrations of aspartate (Fig. S6b), suggesting that opening of Cavs induced by NMDAR activation is not likely to mediate the response to cAMP. These results might be reconciled if a low, constitutive rate of Ca2+ entry through Cavs were to play a permissive role by maintaining the resting Ca2+ concentration within the relevant microdomain close to the threshold for cAMP-induced transcription. Although channels with such properties have not been reported in neurons, a neuronal L-type Ca2+ channel isoform (Cav1.2) that is constitutively active at the resting potential has been described in arterial myocytes (Navedo et al., 2005).
Glutamate receptor-specificity of cAMP-induced aspartate release
The involvement of aspartate as an extracellular messenger in the cAMP/aspartate/NMDAR pathway has potentially interesting implications regarding the specificity of the response because, unlike glutamate, aspartate does not activate either AMPA receptors (Curras and Dingeldine, 1992) or mGluRs (Frauli et al., 2006). If glutamate were the messenger, an increase in cAMP could indiscriminately lead to activation of all ionotropic and metabotropic glutamate receptor subtypes. The central role of extracellular aspartate release in this pathway also raises the interesting possibility of a paracrine signaling mechanism in which an increase in cAMP in one neuron may selectively signal to unstimulated neighboring neurons via NMDARs in the target cells.
DA or cAMP regulation of neuronal development mediated by NMDAR activation
DA, cAMP and NMDARs have separately been implicated in the development of synapses and neuronal circuits (Schmidt et al., 1998; Gomperts et al., 2000; Cai et al., 2001; Shi et al., 2001; Tominaga-Yoshino et al., 2002; Ji et al., 2005; Ultanir et al., 2007; Adesnik et al., 2008). The autocrine signaling mechanism described in this report, in which the regulation of CREB-dependent gene transcription by cAMP is mediated by aspartate activation of NMDARs, may function in developing neural networks to enable cAMP to modulate synaptogenesis and growth of dendrites and axons prior to the formation of functional synapses. It will also be important to determine the extent to which this mechanism persists in the adult nervous system and contributes to the modulation of synaptic plasticity and cognitive function by DA and cAMP.
DA- or cAMP-induced plasticity mediated by NMDAR activation
Many studies have reported that DA or cAMP signaling promotes protein synthesis-dependent LTP and enhances cognitive function in the mature brain (e.g., Frey et al., 1993; Tong et al., 1996; Nguyen and Kandel, 1996; Goldman-Rakic et al., 2000; Nguyen and Woo, 2003; Nie et al., 2007; Isiegas et al., 2008); however, the intracellular signaling cascades that mediate the modulation of synaptic plasticity by cAMP are not well understood. The results reported in this communication suggest that at least some effects of DA and/or cAMP on synaptic plasticity may be mediated by the cAMP/aspartate/NMDAR signaling pathway. Indeed, several reports have described cAMP- or DA-induced changes in plasticity that were blocked by NMDAR antagonists, raising the possibility that the effects of cAMP are mediated by the signaling pathway described here. Otmakhov et al. (2004) reported that the induction of “chemical LTP” by cAMP in hippocampal CA1 pyramidal neurons is mediated by activation of NMDARs. Argilli et al. (2008) found that cocaine-induced plasticity in the ventral tegmental area, which is mediated by activation of AC-coupled D5 DA receptors, requires protein synthesis and is prevented by NMDAR antagonists. Zhang et al. (2009) recently reported that D1/D5 receptor activation modulates spike-timing-dependent plasticity in hippocampal neurons via an NMDAR-dependent pathway. The aspartate+glutamate scavenging system (Fig. 8b) may be useful for determining whether the cAMP/aspartate/NMDAR pathway described here contributes to these DA-, cAMP- and NMDAR-dependent forms of synaptic plasticity.
Supplementary Material
Supp1
Acknowledgments
Funding was provided by grants NIH R01NS048095 and DOD W81XWH-04-1-0176 (BKK), HD16596 (HRZ) and by NIH training grants T32DE007309 (LEFA) T32NS07375 (TJK) and T32GM08181 (PDM). TJK was a BIRCWH Scholar supported by NICHD/ORWH/NIDDK (K12HD43489). The authors gratefully acknowledge Drs. T. W. Abrams and B. E. Alger (University of Maryland School of Medicine, Baltimore, MD) and Dr. D. D. Ginty (Johns Hopkins University School of Medicine, Baltimore, MD) for comments on the manuscript.
  • Adesnik H, Li G, During MJ, Pleasure SJ, Nicoll RA. NMDA receptors inhibit synapse unsilencing during brain development. Proc Natl Acad Sci USA. 2008;105:5597–5602. [PubMed]
  • Argilli E, Sibley DR, Malenka RC, England PM, Bonci A. Mechanism and time course of cocaine-induced long-term potentiation in the ventral tegmental area. J Neurosci. 2008;28:9092–9100. [PMC free article] [PubMed]
  • Arias J, Alberts AS, Brindle P, Claret FX, Smeal T, Karin M, Feramisco J, Montminy M. Activation of cAMP and mitogen responsive genes relies on a common nuclear factor. Nature. 1994;370:226–229. [PubMed]
  • Augustine GJ, Santamaria F, Tanaka K. Local calcium signaling in neurons. Neuron. 2003;40:331–346. [PubMed]
  • Bach ME, Barad M, Son H, Zhuo M, Lu Y-F, Shih R, Mansuy I, Hawkins RD, Kandel ER. Age-related defects in spatial memory are correlated with defects in the late phase of hippocampal long-term potentiation in vitro and are attenuated by drugs that enhance the cAMP signaling pathway. Proc Natl Acad Sci USA. 1999;96:5280–5285. [PubMed]
  • Barco A, Alarcon JM, Kandel ER. Expression of constitutively active CREB protein facilitates the late phase of long-term potentiation by enhancing synaptic capture. Cell. 2002;108:689–703. [PubMed]
  • Bradford SE, Nadler JV. Aspartate release from rat hippocampal synaptosomes. Neurosci. 2004;128:751–765. [PubMed]
  • Brindle P, Nakajima T, Montminy M. Multiple protein kinase A-regulated events are required for transcriptional induction by cAMP. Proc Natl Acad Sci USA. 1995;92:10521–10525. [PubMed]
  • Boulanger L, Poo M. Gating of BDNF-induced synaptic potentiation by cAMP. Science. 1999;284:1982–1984. [PubMed]
  • Cai D, Qiu J, Cao Z, McAtee M, Bregman BS, Filbin MT. Neuronal Cyclic AMP controls the developmental loss in ability of axons to regenerate. J Neurosci. 2001;21:4731–4739. [PubMed]
  • Cardinaux JR, Notis JC, Zhang Q, Vo N, Craig JC, Fass DM, Brennan RG, Goodman RH. Recruitment of CREB binding protein is sufficient for CREB-mediated gene activation. Mol Cell Biol. 2000;20:1546–1552. [PMC free article] [PubMed]
  • Carlezon WA, Jr, Duman RS, Nestler EJ. The many faces of CREB. Trends Neurosci. 2005;28:436–445. [PubMed]
  • Chavez-Noriega LE, Stevens CF. Increased transmitter release at excitatory synapses produced by direct activation of adenylate cyclase in rat hippocampal slices. J Neurosci. 1994;14:310–317. [PubMed]
  • Conkright MD, Guzman E, Flechner L, Su AI, Hogenesch JB, Montminy M. Genome-wide analysis of CREB target genes reveals a core promoter requirement for cAMP responsiveness. Mol Cell. 2003;11:1101–1108. [PubMed]
  • Curras MC, Dingledine R. Selectivity of amino acid transmitters acting at N-methyl-D-aspartate and amino-3-hydroxy-5-methyl-4-isoxazoleproprionate receptors. Mol Pharmacol. 1992;41:520–526. [PubMed]
  • Dash PK, Karl KA, Colicos MA, Prywes R, Kandel ER. cAMP response element-binding protein is activated by Ca2+/calmodulin- as well as cAMP-dependent protein kinase. Proc Natl Acad Sci USA. 1991;88:5061–5065. [PubMed]
  • Deisseroth K, Bito H, Tsien RW. Signaling from synapse to nucleus: postsynaptic CREB phosphorylation during multiple forms of hippocampal synaptic plasticity. Neuron. 1996;16:89–101. [PubMed]
  • Dudman JT, Eaton ME, Rajadhyaksha A, Macias W, Taher M, Kameyama K, Huganir R, Konradi C. Dopamine D1 receptors mediate CREB phosphorylation via phosphorylation of the NMDA receptor at Ser897-NR1. J Neurochem. 2003;87:922–934. [PubMed]
  • Fleck MW, Henze DA, Barrionuevo G, Palmer AM. Aspartate and glutamate mediate excitatory synaptic transmission in area CA1 of the hippocampus. J Neurosci. 1993;13:3944–3955. [PubMed]
  • Frauli M, Neuville P, Vol C, Pin J-P, Prezeau L. Among the twenty classical L-amino acids, only glutamate directly activates metabotropic glutamate receptors. Neuropharmacol. 2006;50:245–253. [PubMed]
  • Frey U, Huang Y-Y, Kandel ER. Effects of cAMP simulate a late stage of LTP in hippocampal CA1 neurons. Science. 1993;260:1661–1664. [PubMed]
  • Goldman-Rakic PS, Muly EC, Williams GV. D1 receptors in prefrontal cells and circuits. Brain Res Rev. 2000;31:295–301. [PubMed]
  • Gomperts SN, Carroll R, Malenka RC, Nicoll RA. Distinct roles for ionotropic and metabotropic glutamate receptors in the maturation of excitatory synapses. J Neurosci. 2000;20:2229–2237. [PubMed]
  • Han EB, Stevens CF. Development regulates a switch between post- and presynaptic strengthening in response to activity deprivation. Proc Natl Acad Sci USA. 2009;106:10817–10822. [PubMed]
  • Hardingham GE, Bading H. Coupling of extrasynaptic NMDA receptors to a CREB shut-off pathway is developmentally regulated. Biochim Biophys Acta. 2002;1600:148–153. [PubMed]
  • Hardingham GE, Arnold FJL, Bading H. A calcium microdomain near NMDA receptors: on switch for ERK-dependent synapse-to-nucleus communication. Nature Neurosci. 2001;4:565–566. [PubMed]
  • Hardingham GE, Chawla S, Cruzalegui FH, Bading H. Control of recruitment and transcription-activating function of CBP determines gene regulation by NMDA receptors and L-type calcium channels. Neuron. 1999;22:789–798. [PubMed]
  • Hardingham GE, Fukunaga Y, Bading H. Extrasynaptic NMDARs oppose synaptic NMDARs by triggering CREB shut-off and cell death pathways. Nature Neurosci. 2002;5:405–414. [PubMed]
  • Huang Y-Y, Kandel ER. D1/D5 receptor agonists induce a protein synthesis-dependent late potentiation in the CA1 region of the hippocampus. Proc Natl Acad Sci USA. 1995;92:2446–2450. [PubMed]
  • Huang Y-Y, Simpson E, Kellendonk C, Kandel ER. Genetic evidence for the bidirectional modulation of synaptic plasticity in the prefrontal cortex by D1 receptors. Proc Natl Acad Sci USA. 2004;101:3236–3241. [PubMed]
  • Impey S, Fong AL, Wang Y, Cardinaux J-R, Fass DM, Obrietan K, Wayman GA, Storm DR, Soderling TR, Goodman RH. Phosphorylation of CBP mediates transcriptional activation by neural activity and CaM kinase IV. Neuron. 2002;34:235–244. [PubMed]
  • Impey S, McCorkle SR, Cha-Molstad H, Dwyer JM, Yochum GS, Boss JM, McWeeney S, Dunn JJ, Mandel G, Goodman RH. Defining the CREB regulon: a genome-wide analysis of transcription factor regulatory regions. Cell. 2004;119:1041–1054. [PubMed]
  • Isiegas C, McDonough C, Huang T, Havekes R, Fabian S, Wu L-J, Xu H, Zhao M-G, Kim J-I, Lee Y-S, Lee H-R, Ko H-G, Lee N, Choi S-L, Lee J-S, Son H, Zhuo M, Kaang B-K, Abel T. A novel conditional genetic system reveals that increasing neuronal cAMP enhances memory and retrieval. J Neurosci. 2008;28:6220–6230. [PMC free article] [PubMed]
  • Ji Y, Pang PT, Feng L, Lu B. Cyclic AMP controls BDNF-induced TrkB phosphorylation and dendritic spine formation in mature hippocampal neurons. Nature Neurosci. 2005;8:164–172. [PubMed]
  • Johnson KB, Criswell HE, Jensen KF, Simson PE, Mueller RA, Breese GR. Comparison of the D1-dopamine agonists SKF-38393 and A-68930 in neonatal 6-hydroxydopamine-lesionsed rats: behavioral effects and induction of c-fos-like immunoreactivity. J Pharmacol Exp Ther. 1992;262:855–865. [PubMed]
  • Jones LB, Stanwood GD, Reinoso BS, Washington RA, Wang H-Y, Friedman E, Levitt P. In utero cocaine-induced dysfunction of dopamine D1 receptor signaling and abnormal differentiation of cerebral cortical neurons. J Neurosci. 2000;20:4606–4614. [PubMed]
  • Kao H-T, Song H, Porton B, Ming G, Hoh J, Abraham M, Czernik AJ, Pieribone VA, Poo M, Greengard P. A protein kinase A-dependent molecular switch in synapsins regulates neurite outgrowth. Nature Neurosci. 2002;5:431–437. [PubMed]
  • Kingsbury TJ, Murray PD, Bambrick LL, Krueger BK. Ca2+-dependent regulation of TrkB expression in neurons. J Biol Chem. 2003;278:40744–40748. [PubMed]
  • Konradi C, Macias W, Dudman JT, Carlson RR. Striatal proenkephalin gene induction: coordinated regulation by cyclic AMP and calcium pathways. Mol Brain Res. 2003;115:157–161. [PubMed]
  • Lonze BE, Ginty DD. Function and regulation of CREB family transcription factors in the nervous system. Neuron. 2002;35:605–623. [PubMed]
  • Lu Y, Li Y, Herin GA, Aizenman E, Epstein PM, Rosenberg PA. Elevation of intracellular cAMP evokes activity-dependent release of adenosine in cultured rat forebrain neurons. Eur J Neurosci. 2004;19:2669–2681. [PubMed]
  • Ma L, Zablow L, Kandel ER, Siegelbaum SA. Cyclic AMP induces functional presynaptic boutons in hippocampal CA3 –CA1 neuronal cultures. Nature Neurosci. 1999;2:24–30. [PubMed]
  • Matthews CC, Zielke HR, Parks DA, Fishman PS. Glutamate-pyruvate transaminase protects against glutamate toxicity in hippocampal slices. Brain Res. 2003;978:59–64. [PubMed]
  • Matthews RP, Guthrie CR, Wailes LM, Zhao X, Means AR, McKnight GS. Calcium/Calmodulin-dependent protein kinase types II and IV differentially regulate CREB-dependent gene expression. Mol Cell Biol. 1994;14:6107–6116. [PMC free article] [PubMed]
  • Mayr B, Montminy M. Transcriptional regulation by the phosphorylation dependent factor CREB. Nature Rev Mol Cell Biol. 2001;2:599–609. [PubMed]
  • Naraghi M, Neher E. Linearized buffered Ca2+ diffusion in microdomains and its implications for calculation of [Ca2+] at the mouth of a calcium channel. J Neurosci. 1997;17:6961–6973. [PubMed]
  • Navedo MF, Amberg GC, Votaw VS, Santana LF. Constitutively active L-type Ca2+ channels. Proc Natl Acad Sci USA. 2005;102:11112–11117. [PubMed]
  • Nguyen PV, Kandel ER. A macromolecular synthesis-dependent late phase of long-term potentiation requiring cAMP in the medial perforant pathway of rat hippocampal slices. J Neurosci. 1996;16:3189–3198. [PubMed]
  • Nguyen PV, Woo NH. Regulation of hippocampal synaptic plasticity by cyclic AMP-dependent protein kinases. Prog Neurobiol. 2003;71:401–437. [PubMed]
  • Nie T, McDonough CB, Huang T, Nguyen PV, Abel T. Genetic disruption of protein kinase A anchoring reveals a role for compartmentalized kinase signaling in theta-burst long-term potentiation and spatial memory. J Neurosci. 2007;27:10278–10288. [PMC free article] [PubMed]
  • O’Brien RJ, Fischbach GD. Modulation of embryonic chick motoneuron glutamate sensitivity by interneurons and agonists. J Neurosci. 1986;6:3290–3296. [PubMed]
  • Otmakhova NA, Lisman JE. D1/D5 dopamine receptors inhibit depotentiation at CA1 synapses via cAMP-dependent mechanism. J Neurosci. 1998;18:1270–1279. [PubMed]
  • Otmakhov N, Khibnik L, Otmakhova N, Carpenter S, Riahi S, Asrican B, Lisman J. Forskolin-induced LTP in the CA1 hippocampal region is NMDA receptor dependent. J Neurophysiol. 2004;91:1955–1962. [PubMed]
  • Parpura V, Haydon PG. Physiological astrocytic calcium levels stimulate glutamate release to modulate adjacent neurons. Proc Natl Acad Sci USA. 2000;97:8629–8634. [PubMed]
  • Pfaffl MW. A new mathematical model for relative quantification in real-time RT-PCR. Nucl Acids Res. 2001;29:2002–2007. [PMC free article] [PubMed]
  • Rajadhyaksha A, Barczak A, Macias W, Leveque JC, Lewis SE, Konradi C. L-type Ca2+ channels are essential for glutamate-mediated CREB phosphorylation and c-fos gene expression in striatal neurons. J Neurosci. 1999;19:6348–6359. [PubMed]
  • Rutledge EM, Kimelberg HK. Release of [3H]-D-aspartate from primary astrocyte cultures in response to raised external potassium. J Neurosci. 1996;16:7803–7811. [PubMed]
  • Schmidt U, Pilgrim C, Beyer C. Differentiative effects of dopamine on striatal neurons involve stimulation of the cAMP/PKA pathway. Molec Cell Neurosci. 1998;11:9–18. [PubMed]
  • Seino S, Shibasaki T. PKA-dependent and PKA-independent pathways for cAMP-regulated exocytosis. Physiol Rev. 2005;85:1303–1342. [PubMed]
  • Shen W, Flajolet M, Greengard P, Surmeier DJ. Dichotomous dopaminergic control of striatal synaptic plasticity. Science. 2008;321:848–851. [PMC free article] [PubMed]
  • Shi J, Aamodt SM, Townsend M, Constantine-Paton M. Developmental depression of glutamate neurotransmissin by chronic low-level activation of NMDA receptors. J Neurosci. 2001;21:6233–6244. [PubMed]
  • Skeberdis VA, Chevaleyre V, Lau CG, Goldberg JH, Pettit DL, Suadicani SO, Lin Y, Bennett MVL, Yuste R, Castillo PE, Zukin RS. Protein kinase A regulates calcium permeability of NMDA receptors. Nature Neurosci. 2006;9:501–510. [PubMed]
  • Tabuchi A, Sakaya H, Kisukeda T, Fushiki H, Tsuda M. Involvement of an upstream stimulatory factor as well as cAMP-responsive element-binding protein in the activation of brain-derived neurotrophic factor gene promoter I. J Biol Chem. 2002;277:35920–35931. [PubMed]
  • Tominaga-Yoshino K, Kondo S, Tamotsu S, Ogura A. Repetitive activation of protein kinase A induces slow and persistent potentiation associated with synaptogenesis in cultured hippocampus. Neurosci Res. 2002;44:357–367. [PubMed]
  • Tong G, Malenka RC, Nicoll RA. Long-term potentiation in cultures of single hippocampal granule cells: a presynaptic form of plasticity. Neuron. 1996;16:1147–1157. [PubMed]
  • Trudeau LE, Emery DG, Haydon PG. Direct modulation of the secretory machinery underlies PKA-dependent synaptic facilitation in hippocampal neurons. Neuron. 1996;17:789–797. [PubMed]
  • Tsien JZ, Huerta PT, Tonegawa S. The essential role of hippocampal CA1 NMDA receptor-dependent synaptic plasticity in spatial memory. Cell. 1996;87:1327–1338. [PubMed]
  • Ultanir SK, Kim J-E, Hall BJ, Deerinck T, Ellisman M, Ghosh A. Regulation of spine morphology and spine density by NMDA receptor signaling in vivo. Proc Natl Acad Sci USA. 2007;104:19553–19558. [PubMed]
  • Vo N, Goodman RH. CREB-binding protein and p300 in transcriptional regulation. J Biol Chem. 2001;276:13505–13508. [PubMed]
  • West AE, Griffith EC, Greenberg ME. Regulation of transcription factors by neuronal activity. Nature Rev Neurosci. 2002;3:921–931. [PubMed]
  • Woodhall GL, Ayman G, Jones RSG. Differential control of two forms of glutamate release by group III metabotropic glutamate receptors at rat entorhinal synapses. Neurosci. 2007;148:7–21. [PMC free article] [PubMed]
  • Wozny C, Maier N, Fidzinski P, Breustedt J, Behr J, Schmitz D. Differential cAMP signaling at hippocampal output synapses. J Neurosci. 2008;28:14358–14362. [PubMed]
  • Xia Z, Dudek H, Miranti CK, Greenberg ME. Calcium influx via the NMDA receptor induces immediate early gene transcription by a MAP kinase/ERK-dependent mechanism. J Neurosci. 1996;16:5425–5436. [PubMed]
  • Xu W, Kasper LH, Lerach S, Jeevan T, Brindle PK. Individual CREB-target genes dictate usage of distinct cAMP-responsive coactivation mechanisms. EMBO J. 2007;26:2890–2903. [PubMed]
  • Yuan SH, Qiu Z, Ghosh A. TOX3 regulates calcium-dependent transcription in neurons. Proc Natl Acad Sci USA. 2009;106:2909–2914. [PubMed]
  • Zhang J-C, Lau P, Bi G. Gain in sensitivity and loss in temporal contrast of STDP by dopaminergic modulation at hippocampal synapses. Proc Natl Acad Sci USA. 2009;106:13028–13033. [PubMed]