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Clin Cancer Res. Author manuscript; available in PMC 2010 July 1.
Published in final edited form as:
PMCID: PMC2802735
EMSID: UKMS28005

A high throughput pharmaceutical screen identifies compounds with specific toxicity against BRCA2-deficient tumors

Abstract

Purpose:

Hereditary breast cancer is partly explained by germline mutations in BRCA1 and BRCA2. While patients carry heterozygous mutations, their tumors have typically lost the remaining wild-type allele. Selectively targeting BRCA-deficiency may therefore constitute an important therapeutic approach. Clinical trials applying this principle are underway, but it is unknown whether the compounds tested are optimal. It is therefore important to identify alternative compounds that specifically target BRCA-deficiency and to test new combination therapies to establish optimal treatment strategies.

Experimental Design:

We performed a high-throughput pharmaceutical screen on BRCA2-deficient mouse mammary tumor cells and isogenic controls with restored BRCA2 function. Subsequently, we validated positive hits in vitro and in vivo using mice carrying BRCA2-deficient mammary tumors.

Results:

Three alkylators – chlorambucil, melphalan and nimustine – displayed strong and specific toxicity against BRCA2-deficient cells. In vivo, these showed heterogeneous but generally strong BRCA2-deficient antitumor activity, with melphalan and nimustine outperforming cisplatin and the poly-(ADP-ribose)-polymerase (PARP) inhibitor olaparib (AZD2281) in this small study. In vitro drug combination experiments showed synergistic interactions between the alkylators and olaparib. Tumor intervention studies combining nimustine and olaparib resulted in recurrence-free survival exceeding 330 days in 3 out of 5 animals tested.

Conclusions:

We generated and validated a platform for identification of compounds with specific activity against BRCA2-deficient cells that translates well to the preclinical setting. Our data call for the re-evaluation of alkylators – especially melphalan and nimustine – alone or in combination with PARP inhibitors for the treatment of breast cancers with a defective BRCA pathway.

Introduction

Breast cancer is the most common malignancy in women. Approximately 10-15% of patients are reported to have a family history of breast cancer [1]. Mutations in the two hereditary breast cancer genes BRCA1 and BRCA2, identified in the mid 90's [2-5], are responsible for 15-20% of familial cases [1]. Although more than a decade of research has significantly increased our knowledge on BRCA gene function, this has not yet led to specific guidelines for the treatment of hereditary breast cancer patients [6].

BRCA1 and BRCA2 play major roles in the error-free repair of DNA double strand breaks (DSBs) through the process of homologous recombination (HR) [7-9]. Breast and ovarian tumors in BRCA-mutation carriers are typically deficient for BRCA function due to loss of heterozygosity [10, 11]. This inspired researchers to exploit the DNA repair defects of BRCA-mutated tumors by treating them with DNA damaging agents that either directly or indirectly induce DSBs.

Two classes of drugs that recently gained momentum in the treatment of BRCA-associated patients are platinum based DNA cross-linking agents and inhibitors of poly-(ADP-ribose) polymerase (PARP) activity. Cisplatin and carboplatin mostly induce inter- and intrastrand cross-links, but processing of these lesions results in intermediates of which the resolution depends on intact HR. This is thought to explain the strong sensitivity of BRCA1- and BRCA2-deficient cells towards these drugs [12, 13].

PARP activity is necessary for efficient single strand break (SSB) repair by the base excision repair (BER) pathway. In the absence of PARP activity, these lesions are thought to be converted into DSBs when encountered by replication forks that are unable to proceed. Whereas wild-type cells can subsequently repair these DSBs by HR, BRCA-deficient cells will accumulate unrepaired DSBs or misrepaired lesions due to promiscuous activity of error-prone repair pathways, ultimately leading to mitotic catastrophe. Indeed, small molecule inhibitors of PARP enzymatic activity have been shown to be highly toxic to BRCA-deficient cells [14, 15].

Both platinum-based drugs and olaparib (AZD2281), a PARP inhibitor recently shown to kill BRCA-deficient mouse mammary tumor cells [16, 17] and with promising results from phase 0 and phase 1 trials [18, 19], are currently in phase II clinical trials in breast cancer patients [20]. While clinical trials usually take several years to complete, experimentation using animal models has already yielded several important insights. BRCA1-deficient mammary tumors responded well when treated with cisplatin, carboplatin or olaparib [17, 21] and also BRCA2-deficient mammary tumors regress upon treatment with olaparib [22]. It seems unlikely, however, that these drugs will prove to be the ultimate answer to treating BRCA patients. In the same studies, Rottenberg et al. found that tumors develop resistance against olaparib and that treating with platinum drugs does not fully eradicate the tumor. Even combination treatment of olaparib with platinum agents resulted in tumor relapses, albeit with a longer latency [17, 22]. Hence, there is a continuing need for new drugs or drug combinations that selectively kill BRCA-deficient tumor cells.

In this paper, we report the development of an in vitro high-throughput screening system to identify compounds with specific toxicity towards BRCA2-deficient cancer cells. We have used this system to probe a commercially available library of 1258 drugs with known pharmacological activity and found, besides carboplatin, three alkylating agents: chlorambucil, melphalan and nimustine. In vitro validation experiments showed strong selective activity of these compounds against BRCA2-deficient cells and specific synergy with olaparib. In vivo studies using single-agent therapy showed favorable relapse-free survival times for two of the three compounds compared to cisplatin and olaparib in two different mouse models for BRCA2-deficient breast cancer. Combination therapy with nimustine and olaparib resulted in a relapse-free survival of more than 330 days in 3 out of 5 animals tested. Besides offering a platform for the identification of additional compounds for treatment of BRCA related cancers, these results call for a re-evaluation of alkylating agents already known to the clinic but not generally used for the treatment of breast cancer and further assessment of combination therapies containing both alkylators and PARP inhibitors.

Materials and Methods

Cell line culturing and BRCA2 reconstitution

KP3.33, KP6.3, KB2P1.21 and KB2P3.4 mouse mammary tumor cell lines [16] were cultured at 37°C, 5% CO2, 3% O2 in complete medium (CM, DMEM/F-12 (Life Technologies) supplemented with 10% FCS, 50 units/ml penicillin, 50 μg/ml streptomycin (Life Technologies), 5 μg/ml insulin (Sigma), 5 ng/ml epidermal growth factor (Life Technologies) and 5 ng/ml cholera toxin (Gentaur)). To reconstitute the BRCA2-deficient cell lines, HSV-1/EBV amplicons containing the complete mouse Brca2 gene were produced as described previously [23]. 40x Concentrated Brca2 iBACs were aliquoted and stored at −80°C until use. After overnight infection and subsequent 48hr recovery, Brca2 iBAC containing KB2P cells were selected with 400μg/ml hygromycin. RT-PCR and RAD51/γH2A.X co-localization experiments to validate functional BRCA2 reconstitution were performed as described [16].

Growth inhibition assays

All single-drug in vitro experiments were performed in 96-well plates. Typically, 150 KP3.33, 100 KP6.3, 400 KB2P1.21, 400 KB2P1.21R2, 500 KB2P3.4 and 300 KB2P3.4R3 cells were plated in 160μl CM on day 0. On day 1, 40μl drug containing CM was added while maintaining DMSO concentrations similar in all wells and <0.4%. On day 5, cells were incubated with 10μl cell-titer blue (Promega) for 4 hours at 37°C. Fluorescence was measured at 590 nm using an Envision plate reader (Perkin Elmer). Values from triplicate measurements were averaged, corrected for background and normalized against values from DMSO only controls. IC50 Values were calculated using Matlab software, as described [16] . All drug interaction experiments were done with SRB assays, since these were less prone to signal variation, which is crucial to accurate determination of combination indices. Combination index values were determined as described [16].

Screen set-up

10mM DMSO Stocks of the LOPAC1280 compound library (Sigma) were used to create 5mM and subsequent ten-fold dilutions in DMSO. Cells were seeded on day 0 in 384-well plates in 40μl CM. On day 1, 96-well plates were prepared with 100-fold diluted drugs in CM in columns 2-11. Columns 1 and 12 were used for DMSO-only negative controls and multiple olaparib concentrations as positive controls. Assay plates were supplied with 10μl diluted compound. On day 5, a 4-hour incubation with 20μl 8x diluted cell-titer blue was used to determine cell viability. Screening was done using five 10-fold concentration steps, ranging from 1nM to 10μM. Mean values of technical duplicates were standardized against DMSO-only controls in the same plate and IC50 values were calculated. When IC50 values were >50μM or when the maximum growth inhibition of any range did not exceed 25%, IC50s were arbitrarily set at 50μM.

In vivo validation studies

Chlorambucil (Sigma) was dissolved in 70% EtOH to 50mg/ml. Immediately prior to use, this stock was diluted 40x with 100mM Phosphate buffer pH6.8. Melphalan (Alkeran®, GlaxoSmithKline) was reconstituted immediately before administration, according to manufacturer's protocol. 1 Gram nimustine hydrochloride (Sigma) was dissolved in 6.5ml DMSO and just before injection, diluted 25-fold with physiological salt solution. Cisplatin was obtained from (Mayne Pharma). olaparib was kindly provided by KuDOS Pharmaceuticals [24]. Olaparib, Chlorambucil, Melphalan and nimustine were injected intraperitoneally. Cisplatin was injected intravenously in the tail vein.

Since all cells and tumors were derived from mice with a mixed FVB and 129/Ola background, (FVB × 129/Ola) F1 hybrid females were used for all animal experiments reported in this paper. Maximum tolerable doses (MTDs) were determined using dose escalation in at least three animals for all three alkylators and cisplatin using a 20% weight loss as a toxicity threshold. The following MTDs were established: chlorambucil: 15mg/kg i.p.; melphalan: 7.5mg/kg i.p.; nimustine: 30mg/kg i.p.; cisplatin: 6mg/kg i.v.. olaparib was administered daily at 100mg/kg i.p., at which no toxicity was observed, but higher amounts would exceed the maximum of 1ml daily injection volume. For the tumor intervention studies, either 500.000 KB2P3.4 cells, or 1mm3 pieces of 5 independent mammary tumors from K14cre;Brca2F11/F11;p53F2-10;F2-10 animals were engrafted orthotopically in the 4th mammary fat pad of adult F1 hybrid mice as described [21]. Treatment at MTD levels was started once tumor volume, calculated as 0.5 × length × width2, exceeded 200mm3. Since olaparib does not show any toxicity at 100mg/kg and since after 24 hours only about 1/3 of the maximum PARP inhibition remains due to rapid elimination [17], we decided to dose olaparib daily for a period of 28 consecutive days. When the tumor volume was greater than 1500mm3, the animal was sacrificed. All animal experiments were done in accordance with institutional guidelines and local regulations.

Results

Reconstitution of BRCA2-deficient mouse mammary tumor cells

We previously isolated a set of BRCA2-proficient (KP) and BRCA2-deficient (KB2P) mouse mammary tumor cell lines with differential responses to several agents such as cisplatin and the PARP inhibitor olaparib [16]. These cell lines are not suitable to perform pharmaceutical screens, because many other mutations may account for any specific toxicity found. Reconstituting the BRCA2-deficient KB2P-1.21 and KB2P-3.4 cell lines, resulting in isogenic cell line pairs with differential BRCA2 functionality, should overcome this problem. However, artificial BRCA2 expression from cDNA constructs was shown to severely impair cell growth [25]. We therefore chose to reconstitute the complete Brca2 gene including its endogenous promoter by stable introduction of a BAC clone encompassing the mouse Brca2 locus. Both KB2P lines were reconstituted with an HSV-1 based Brca2 infectious BAC (iBAC) [23], which led to reconstitution of full-length Brca2 expression as shown by RT-PCR analysis (KB2P3.4R3 and KB2P1.21R2, Figure 1A). Array CGH analysis confirmed the isogenicity of the BRCA2-deficient cell lines and their reconstituted counterparts (Supplementary Figure 1). Rescue of RAD51 colocalization with γH2A.X in γ-irradiated KB2P3.4R3 and KB2P1.21R2 cells showed that the reconstitution was functional (Figure 1B). To further test the utility of these cell lines for pharmaceutical screens, we performed growth inhibition assays for olaparib and cisplatin on the KB2P, KB2PR and KP cell lines (Figure 1C). BRCA2 Reconstitution completely reversed sensitivity to these compounds to similar levels as in the BRCA2-proficient KP lines (KB2P1.21R2) or to even higher levels (KB2P3.4R3). This hyperresistance correlated with higher Brca2 expression levels in KB2P3.4R3 cells compared to KP control cells as measured by qRT-PCR (data not shown). Since the window of differential sensitivity appeared highest in the KB2P3.4/KB2P3.4R3 combination, we used this isogenic pair of cell lines to perform a high-throughput pharmacological screen. As an independent control, we used the BRCA2-proficient KP3.33 cell line [16].

Figure 1
Functional reconstitution of BRCA2-deficient mammary tumor cells

A high throughput screen for compounds with specific cytotoxicity against BRCA2-deficient cells

Using a high-throughput robotic screening system, we exposed KP3.33, KB2P3.4 and KB2P3.4R3 cells to a library of clinically active compounds (LOPAC, Sigma) containing 1258 compounds. Ninety-seven drugs had an IC50 <50μM in any of the cell lines used (Figure 2A). To determine the influence of BRCA2 expression on the IC50 values, a combined specificity index (CSI) was calculated by addition of the log10 differential IC50 values for KB2P3.4 vs. KB2P3.4R3 and KB2P3.4 vs. KP3.33, respectively (see Supplementary Table 1). Carboplatin, which is currently used in clinical trials in BRCA-associated breast cancer patients, was the fifth best hit with an IC50 of 4.4μM in the KB2P3.4 cells, but with no significant growth inhibition in the BRCA2-proficient cells. Also the topoisomerase inhibitors camptothecin and ellipticine showed a considerable differential cytotoxicity, which is in accordance with previously published work [26]. Inhibition of topoisomerase activity is known to result in DNA strand breaks, which could explain the activity of these drugs.

Figure 2
A pharmacological screen identifies three alkylators with specific toxicity to BRCA2-deficient cells

In vitro validation of compounds

Four compounds (chlorambucil, melphalan, nimustine, 2-Cyclooctyl-2-hydroxyethylamine (CONH)) reported an even higher CSI than carboplatin. Except for CONH, which did not validate in subsequent experiments (data not shown), these compounds were alkylating agents not currently part of the standard of care for treating breast cancer patients [27]. To further validate the efficacy of these drugs, we determined growth inhibition curves of freshly dissolved compounds on our complete panel of cell lines. Indeed, for all three alkylators, both BRCA2-deficient cell lines showed much greater sensitivity than the BRCA2 reconstituted and –proficient controls (Figure 2B). Immunofluorescent staining revealed clear co-localization of γH2A.X and RAD51 foci after treating KP3.33 cells for 24hrs with the alkylators at IC50 concentrations. This confirms that these drugs are capable of inducing recombinogenic DNA breaks, which are known substrates for BRCA2 function.

In vitro synergism between alkylators and PARP inhibitor olaparib

Combinations of the DNA cross-linker cisplatin with the PARP inhibitor olaparib were previously shown to synergistically inhibit the growth of BRCA2-deficient cancer cells [16]. We assessed drug interactions between olaparib and chlorambucil, melphalan or nimustine in our panel of cell lines by determining combination index values for a range of combined drug concentrations. The means of all combination indices (CIs) of two independent experiments were calculated and averaged (Table 1). Synergistic drug interactions, corresponding to CIs of less than −0.15 [28], were found for chlorambucil, melphalan and nimustine in the BRCA2-deficient KB2P1.21 cell line and are comparative to an average cisplatin/olaparib CI of −0.16 [16]. For chlorambucil and nimustine, synergy was also found in the KB2P3.4 line at an even higher level than for cisplatin, which was previously shown to have an average CI of −0.17 [16]. Interestingly, combinations of alkylating agents with olaparib in the BRCA2-proficient cell lines resulted in additive effects rather than synergistic interactions. Since drug synergism was thus found to be specific for BRCA2-deficient cells, combining these drugs could further increase an existing clinical window in patients with BRCA-deficient tumors.

Table 1
Drug interactions between olaparib and alkylators

In vivo responses of orthotopically transplanted BRCA2-deficient mammary tumor cells

To further substantiate our findings, we performed in vivo validation studies with chlorambucil, melphalan and nimustine in mice carrying BRCA2-deficient tumors. Upon orthotopic transplantation of 500,000 KB2P3.4 cells in the 4th mammary fat-pad of young adult female mice, palpable tumors developed in approximately 1.5 months. Drug treatment was started when tumors exceeded 200mm3. Alkylators were administered only once, while olaparib was administered daily during 28 consecutive days.

Tumor bearing animals that were left untreated had to be sacrificed after 1-2 weeks, when tumors reached a size of approx. 1500 mm3 (Figure 3). Efficacy of PARP inhibition as an approach to target BRCA2-deficient tumor cell growth is clearly shown, although responses were heterogeneous and tumors progressed quite rapidly after initial response to olaparib (Figure 3). On the contrary, treatment with a single dose of cisplatin or any of the alkylators invariably resulted in partial or even complete tumor remission (Figure 3). Relapses nevertheless occurred in all cisplatin and chlorambucil treated animals within a 45-day period. Melphalan treated animals, however, survived longer (61 days on average vs. 38 for cisplatin and 45 for chlorambucil). Strikingly, all animals treated with nimustine remained relapse free for at least 100 days.

Figure 3
In vivo validation of drug activity on orthotopically transplanted cell-lines

Treatment responses of allografted BRCA2-deficient mammary tumors

Although orthotopic allografting of BRCA2-deficient mammary tumor cell lines into the mouse mammary fat pad constitutes an efficient way of determining targeted drug efficacy in vivo, differences between spontaneous tumors and tumor cell line outgrowths may exist due to prolonged in vitro culturing and the potential absence of an intact stromal compartment. These issues may be overcome by direct engraftment of tumor pieces into multiple syngeneic animals, resulting in secondary tumors that closely mimic the parental tumors [21]. Similar to these studies, we engrafted tissue pieces originating from a panel of 5 independent mammary tumors from K14cre;Brca2F11/F11;p53F2-10;F2-10 mice [29] into the mammary gland fat pads of multiple recipients (Figure 4A). When left untreated, mice needed to be sacrificed 1-2 weeks after diagnosis of a palpable tumor, due to rapid, expansive growth of the local tumor. The engrafted tumors showed varying responses to the PARP inhibitor olaparib. While some tumors (Figure 4A, tumors 1 and 4) hardly responded and even needed to be sacrificed before the end of the 28-day treatment schedule, other tumors showed a partial response (Figure 4A, tumors 3 and 5) or even complete remission before eventual relapse (Figure 4A, tumor 2). In line with the results of the cell line allografts, tumor grafts generally showed stronger responses to cisplatin, chlorambucil, melphalan and nimustine than to olaparib. Again, melphalan and nimustine resulted in better relapse free survival after treatment compared to olaparib and cisplatin (Figure 4B). Of note, two animals of our nimustine-treated tumor graft cohort needed to be sacrificed before having relapses, approximately 6 months after treatment. Histological analyses showed renal failure to be the most likely cause of death. Despite the relatively small size of the tumor cohort, borderline significant log-rank p-values were determined for olaparib vs. melphalan/nimustine and for cisplatin vs. melphalan.

Figure 4
In vivo validation of drug activity on allografted BRCA2-deficient tumors

Besides these favorable results, complete cure seems still infrequent. Only one animal, treated with Melphalan, is still recurrence free 290 days after the start of treatment. To see whether the in vitro synergy between the alkylators and olaparib translates in vivo, we have treated mice harboring allografts of the same tumors as used in the single agent experiments with combination therapy. Unfortunately, combining single dose Melphalan at MTD with a 28-day olaparib course was associated with increased toxicity, precluding analysis of this combination at dosages comparable to their single agent treatment regimes. However, when single dose nimustine treatments were combined with 28-days of olaparib treatment, only two out of five animals suffered from recurrences in a period currently exceeding 330 days.

Discussion

Knowledge of the role of BRCA1 and BRCA2 in DNA damage repair has greatly accumulated in recent years. It can be expected that this knowledge will ultimately translate into treatment protocols that discriminate between sporadic cancers and BRCA-associated or BRCA-like tumors. Indeed, clinical trials with conventional and novel anticancer drugs targeting homologous recombination deficiency (HRD) are currently ongoing [20]. Both approaches taken in these clinical studies - inhibition of BER by PARP inhibitors and DNA damage induction by platinum-based cross-linkers - are thought to induce DSBs in replicating cells [14, 15, 30]. It is unknown, however, whether these agents are the most suitable compounds to reach this goal. Ideally, HRD targeting compounds should generate lesions that are only toxic to HRD cells without inducing DNA damage toxic to normal cells.

To identify novel compounds with specific toxicity towards BRCA2-deficient cells, we have developed a discovery pipeline consisting of isogenic cell lines suitable for high throughput in vitro screening, as well as in vivo models for validation studies. We found that three alkylators, which are currently not used in the treatment of breast cancer patients, exert strong differential in vitro cytotoxicity against BRCA2-deficient mammary tumor cells versus isogenic BRCA2-proficient control cells. In vivo validation studies in mice engrafted with BRCA2-deficient tumor cell lines or primary tumors showed very strong antitumoral activity of all three alkylators especially given the fact that these compounds were administered only once. In these mouse models, melphalan and nimustine outperformed olaparib and cisplatin, although tumor cohorts were relatively small, precluding definitive statistical conclusions. However, these findings may have clinical importance since olaparib and the platinum compound carboplatin are currently in phase II clinical trials.

All three compounds identified are bifunctional alkylating agents, with melphalan and chlorambucil belonging to the class of nitrogen mustards and nimustine being a nitrosourea compound. Bifunctional alkylators create both inter- and intrastrand cross-links in double stranded DNA. Platinum compounds such as cisplatin and carboplatin have similar activity but are thought to be different from most bifunctional alkylators because they mainly produce intrastrand cross-links instead of interstrand cross-links [30, 31]. Intrastrand lesions are repaired by nucleotide excision repair (NER) [32]. Thus, the differential sensitivity of HR-deficient vs. HR-proficient cells towards platinum compounds may actually be explained by a minority of the lesions produced, with many intrastrand cross-links as ‘collateral damage’. These intrastrand cross-links may be selectively toxic in some HRD tumors, but not in others. Besides its role in HR, BRCA1 is also involved in NER [33]. BRCA1-associated tumors may thus be sensitive to intrastrand cross linking agents. BRCA2, on the other hand, is not implicated in NER. Although p53 is involved in NER [34] and many BRCA2 tumors have TP53 mutations, also BRCA2 tumors exist that have wild-type TP53 [35]. Treating these HR-deficient but NER-proficient BRCA2-associated tumors with platinum compounds may thus prove to be suboptimal due to the specific spectrum of DNA adducts formed. In addition, resistance mechanisms may differ between platinum compounds and bifunctional alkylators.

The use of melphalan and chlorambucil in the treatment of breast cancer is not entirely new. Especially poorly differentiated tumors showed favorable response to single-agent melphalan therapy[36]. However, regimens using melphalan or chlorambucil did not perform any better than the standard of care combination treatment at the time, cyclophosphamide, methotrexate and 5-fluorouracil (CMF)[37, 38], and these alkylators were therefore abandoned as treatment options. While nimustine is mainly used in Japan for the treatment of malignant glioma, we are not aware of any clinical trials with this compound in breast cancer patients. Related compounds such as BCNU (carmustine) and CCNU (lomustine) have been part of drug combinations used in several clinical trials. Interestingly, carmustine was part of the LOPAC library but did not result in significant toxicity in BRCA2-deficient KB2P3.4 cells, even at 10μM concentration.

Importantly, early clinical trials with melphalan, chlorambucil and nitrosoureas have only been carried out in cohorts of breast cancer patients not selected for certain molecular properties. Strong specific response in patients with HRD tumors may thus have been obscured by absence of response in the majority of (HR-proficient) patients. In light of the strong preclinical responses reported in this study, re-evaluation of ‘classical’ alkylators in HRD tumors should be considered. Apart from BRCA-associated hereditary breast cancers, hormone receptor- and HER2-negative sporadic breast tumors (so-called “triple negative” tumors) are also believed to harbor a considerable fraction of HRD tumors with BRCA-like characteristics [39-41]. Although identification of sporadic HRD tumors remains problematic, promising results have been obtained with an array-based comparative genomic hybridization (aCGH) classifier able to identify BRCA1 tumors ([42, 43]). Recently, this BRCA1-like aCGH pattern was shown to be significantly associated with a prolonged progression-free survival of metastatic breast cancer patients treated with high-dose alkylating chemotherapy with cisplatin, thiotepa and cyclophosphamide (Vollebergh et al, submitted and [44]).

Our results show that mammary tumor cells are sensitive to cisplatin or the PARP inhibitor olaparib in a BRCA2 dependent fashion, further supporting the rationale for ongoing clinical trials with these compounds. In addition to favorable single agent activity of olaparib against BRCA2-deficient mammary tumors, we also observe in vitro synergy between olaparib and the alkylators, specifically in BRCA2-deficient cells. In vivo, we observed very long recurrence-free survival – suggestive of complete tumor eradication – in 3 out of 5 animals treated with a combination of nimustine and olaparib. Combining olaparib with bifunctional alkylators may thus further increase the clinical window for the treatment of BRCA-deficient cancers.

We introduce here a cell-based screening and validation system for identification and preclinical in vitro and in vivo validation of compounds with specific toxicity against mammary tumor cells with a defined genetic lesion, i.e. BRCA2 deficiency. Our strategy, based on isogenic pairs of tumor cell lines, is in principle applicable to other tumor suppressor genes, provided that the reconstitution does not affect proliferation and/or survival of tumor cells. Our results obtained with isogenic BRCA2-deficient and –proficient mammary tumor cell lines call for a re-evaluation of melphalan and nimustine as single agents or in combination with PARP inhibitors, in the clinical management of BRCA2-deficient breast cancer.

Translational relevance

We present a cell-line based platform for high-throughput identification of chemical compounds with specific toxicity towards BRCA2-deficient tumor cells. This method can be used to assess specific toxicity of known drugs and to identify new classes of compounds targeting as yet unknown aspects of BRCA-deficient cancer. Using this platform, we find strong BRCA2-deficient antitumor activity of three alkylating agents, which were previously abandoned in clinical trials on unselected patient populations. These alkylating compounds have synergistic interaction with poly-(ADP-ribose)-polymeration (PARP) inhibitors, which are currently in clinical trials for the treatment of hereditary breast cancer patients. Our results show the importance of re-evaluating alkylating agents in breast cancer patients with BRCA2-like defects. In addition to hereditary predisposed individuals, these may include patients with homologous recombination deficient (HRD) sporadic breast cancer.

Supplementary Material

Acknowledgments

We thank KuDOS Pharmaceuticals, a wholly owned subsidiary of AstraZeneca, for providing olaparib. Dr Y. Saeki and Dr R. M. Sandri-Goldin are kindly acknowledged for providing reagents for iBAC production. This work was supported by grants from the Dutch Cancer Society (2002-2635 to J.J. and A. Berns; 2007-3772 to J.J., S. Rottenberg and J.H.M. Schellens), the Netherlands Organization for Scientific Research (NWO Vidi 917.036.347 to J.J.) and by the Biotechnology and Biological Science Research Council UK (BB/D012910/1 to RWM). D.J.A. is supported by Cancer Research-UK and the Wellcome Trust. Hanneke van der Gulden is acknowledged for her technical assistance. We thank Piet Borst, Michael Hauptmann, Huib Ovaa, Sjoerd Rodenhuis, Boris Rodenko and Marieke Vollebergh for providing useful comments on the manuscript.

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