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As long as we keratinocytes can remember, melanocytes have lived inconspicuously among us at the basal membrane and have been primarily known as meek and obedient pigment producers in the epidermis. However, recent investigations are suggesting that these fellows have several identities, work undercover in many other places in the human body and have functions we can only speculate about.
The purpose of this essay is to consider the various aspects of the well-known and the more obscure abilities of melanocytes.
First of all, how does one recognize a melanocyte if it is encountered somewhere outside the epidermis? They are usually identified by their expression of melanocyte-specific proteins e.g. tyrosinase (TYR), TYRP1, DCT, Pmel17/gp100, MART-1 and/or MITF. However, melanocyte precursors (known as melanoblasts) are more difficult to identify since they don’t produce melanin and therefore don’t usually express those markers, although occasionally DCT and/or KIT are detectable as specific markers.
The favorite habitat of melanocytes is the epidermis, but large numbers of them can be found in hair follicles and in the eyes where they manufacture melanin for hair and eye pigmentation, respectively, and in other, less well-known locations as discussed below. The fact that we keratinocytes control melanocytes in the skin via an armamentarium of growth factors  has led to the impression that keratinocytes and melanocytes live in a sort of master-slave relationship. However, the dependency is not unilateral at all; melanocytes transport melanin in membrane-bound organelles (termed melanosomes) via their elongated dendrites and then transfer them to us  whereupon we arrange them to form a critical protective barrier (known as supranuclear ‘caps’) to shield our DNA from UV radiation .
Melanocytes (and melanin) also function early during human development; they play critical roles during embryonic development as can be seen in individuals with oculocutaneous albinism type 1 (OCA1). OCA1 results from the dysfunction of TYR which leads to impaired pigmentation of skin, hair and eyes  but also to misrouting of the optic nerves at the chiasm . Melanocytes express the melanocortin 1 receptor (MC1R) that regulates the quality and quantity of their melanin production. MC1R is controlled by the agonists melanocyte-stimulating hormone (MSH) and adrenocorticotropic hormone (ACTH) , which stimulate the melanogenic cascade and thus the synthesis of eumelanin, as well as by the antagonist agouti signaling protein (ASP) . It is known that ASP elicits the production of pheomelanin, but it was shown only recently that ASP also modulates the expression of genes involved in morphogenesis (especially in nervous system development) .
Besides their existence in the skin, melanin and melanocytes have been reported to appear in the stria vascularis of the cochlea , in the leptomeninges , substantia nigra and locus coerulus  of the brain, in the heart [12,13] and there is evidence that they operate even in such inhospitable territories as adipose tissue .
Note in passing that there are two distinct types of melanocytes: differentiated melanocytes that originate from the neural crest and can be found at various locations in the body, and a second type, the retinal pigment epithelium (RPE), specifically present only as a single layer of cells lying behind the retina that develop in situ from the optic cup of the brain . The RPE plays a critical role in the active phagocytosis and turnover of the rod outer segments of the retina as well as in the uptake, processing and transport of retinoids and consequently has an important function in vision .
Melanocytes are also present as intermediate cells in the stria vascularis of the cochlea. Strial intermediate cells are required for the generation of endolymph-mediated action potentials that are necessary for normal hearing . Hearing impairment can be associated with inherited pigmentary disorders, e.g. Waardenburg syndrome , and studies have shown that the extent of induced temporary hearing loss is inversely related to skin pigment type . Melanin granules produced by melanocytes in the inner ear even play important roles in balance .
Extracutaneous melanocytes located in the brain may have several neuroendocrine functions. Human melanocytes express lipocalin-type prostaglandin D synthase (L-PGDS) which generates prostaglandin D2 (PGD2) . Besides, in melanocytes, β-endorphin, an endogenous opioid, is generated from proopiomelanocortin (POMC) together with MSH and ACTH. Does this suggest that melanocytes regulate sleep? PGD2 is a potent sleep-inducing substance  and opioid receptors are located in the nuclei that are active in sleep regulation . Also, there are indications that a certain melanocyte-derived factor might be involved in controlling the central chemosensor that generates the respiratory rhythm . Pigment in the brain, termed neuromelanin, consists of a large, complex, eumelanin-covered pheomelanin core which may also contain aliphatics and peptides . Neuromelanin is primarily localized in dopaminergic neurons of the substantia nigra and in the locus coerulus, and accumulates in the human substantia nigra with age . A selective loss of dopaminergic neurons containing neuromelanin is associated with Parkinson’s disease . Various studies support the concept that neuromelanins have a protective function by binding/removal of ROS and metals that would otherwise be highly toxic to neurons [11,23,24]. A recent study showed that virtually all brain tissues contain significant amounts of neuromelanin, which are thought to play important roles in reducing toxicity in those tissues .
It has also been brought to our attention that melanocytes are located in the valves and septa of the heart [12,13]. Mice presenting with hyper (hypo-) pigmented skin show increased (or decreased) heart pigmentation . Cardiac melanocytes may originate from the same precursor population as skin melanocytes as they depend on the same the signaling molecules known to be required for proper skin melanocyte development , but their function in this location so far is obscure. It may well turn out that the production of melanin is not always of benefit, either in the heart or in other tissues, such as the lungs, where in a rare disease known as LAM , muscle cells revert towards their developmental origins and express some melanocyte markers, such as tyrosinase, Pmel17, etc. The resulting production and accumulation of melanin in lung tissues is eventually lethal.
Recently, we have learned that melanin biosynthesis also takes place in the visceral adipose tissue of morbidly obese humans . Hypothetically, the ectopic synthesis of melanin in the cytosol of obese adipocytes may serve as a compensatory mechanism to act as an anti-inflammatory factor and to reduce oxidative damage. During increases of cellular fat deposition, adipocytes become more exposed to endogenous apoptotic signals, especially ROS, which could be counteracted by ectopically produced melanin. In addition, adipocytic melanin may also suppress the secretion of proinflammatory molecules .
In conclusion, we think it unfair that melanocytes reap all the glory for their role in pigmenting the skin and providing it critical protection against UV damage, when in fact it is us as keratinocytes that form the bulk population of that tissue and deserve all the credit. It adds insult to injury that melanocytes are now beginning to take more and more glory for their roles in other tissues of the body. Where will it all end?