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Logo of bmcgenoBioMed Centralsearchsubmit a manuscriptregisterthis articleBMC Genomics
 
BMC Genomics. 2009; 10: 573.
Published online Dec 2, 2009. doi:  10.1186/1471-2164-10-573
PMCID: PMC2792230
Robustness of transcriptional regulatory program influences gene expression variability
Zhiming Dai,1 Xianhua Dai,corresponding author1 Qian Xiang,1 and Jihua Feng1
1Electronic Department, Sun Yat-Sen University, Guangzhou, PR China
corresponding authorCorresponding author.
Zhiming Dai: zhimdai/at/gmail.com; Xianhua Dai: issdxh/at/mail.sysu.edu.cn; Qian Xiang: xiangq/at/mail.sysu.edu.cn; Jihua Feng: fengjihua/at/21cn.com
Received September 7, 2009; Accepted December 2, 2009.
Abstract
Background
Most genes are not affected when any transcription factor (TF) is knocked out, indicating that they have robust transcriptional regulatory program. Yet the mechanism underlying robust transcriptional regulatory program is less clear.
Results
Here, we studied the cause and effect of robust transcriptional regulatory program. We found that cooperative TFs in the robust transcriptional regulatory program regulate their common target genes in an activity-redundant fashion, and they are able to compensate for each other's loss. As a result, their target genes are insensitive to their single perturbation. We next revealed that the degree of robustness of transcriptional regulatory program influences gene expression variability. Genes with fragile (unrobust) transcriptional regulatory program under normal growth condition could be readily reprogrammed to significantly modulate gene expression upon changing conditions. They also have high evolutionary rates of gene expression. We further showed that the fragile transcriptional regulatory program is a major source of expression variability.
Conclusion
We showed that activity-redundant TFs guarantee the robustness of transcriptional regulatory programs, and the fragility of transcriptional regulatory program plays a major role in gene expression variability. These findings reveal the mechanisms underlying robust transcription and expression variability.
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