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Treatment of xerostomia would benefit from development of a functional implantable artificial salivary gland. Salivary gland tissue from surgical patients was assessed by histology and immunohistochemistry to establish the phenotype of normal salivary gland cells including the native basement membranes. Ductal and acinar cells were identified in tissue and cultured cells from dispersed tissue. High levels of laminin and perlecan/HSPG2 (heparan sulfate proteoglycan 2) were noted in basement membranes, and perlecan also was secreted and organized by cultured acinar populations, which formed lobular structures that mimicked intact glands when cultured on Matrigel™ or a bioactive peptide derived from domain IV of perlecan. On either matrix, large acini-like lobular structures grew and formed connections between the lobes. α-Amylase secretion was confirmed by staining and activity assay. Biomarkers, including tight junction protein E-cadherin and water channel protein aquaporin 5 found in tissue, were expressed in cultured acinar cells. Cells cultured on Matrigel or domain IV of perlecan peptide organized stress fibers and activated focal adhesion kinase. We report a novel technique to isolate acinar cells from human salivary gland and identify a human peptide sequence in perlecan that triggers differentiation of salivary gland cells into self-assembling acini-like structures that express essential biomarkers and which secrete α-amylase.
Xerostomia is a permanent and devastating sequela of head and neck radiation that affects approximately 40,000 patients annually in the United States.1 Direct radiation damage of the acinar cells that secrete fluid and protein results in salivary gland hypofunction. Histopathologic and immunohistochemical studies of chemoradiated salivary glands have shown profound acinar cell loss that can be attributed to lethal DNA damage under conditions in which ductal cells are preserved.2 Selective loss of acinar cells compromises the quantity and quality of saliva and produces conditions such as xerostomia, dysphagia, dental caries, mucositis, and other oropharyngeal infections. Patients suffer considerable morbidity, and their quality of life deteriorates significantly.1 Present treatments are unsatisfactory. We envision the creation of an implantable artificial salivary gland that can aid these patients to regain salivary functions.
Other groups have reported the isolation and culture of human salivary gland cells for tissue engineering purposes. Isolation of primary cells from primate and human salivary glands permitted the growth of ductal cells.3 While these cells are epithelial and can form tight junctions, they do not secrete fluid or the full array of salivary proteins produced by acinar cells.3,4 Recent studies have reported the successful isolation of human salivary acinar cells that express many essential markers.5,6 Joraku et al. reported reconstitution of salivary units that expressed α-amylase and an array of tight junction markers in a culture system consisting of collagen and Matrigel™.5 Although promising, this system cannot be used for human tissue engineering because Matrigel, being mouse derived, is not compatible with human systems. Recently, it was reported that a similar isolation of acinar cells required the use of animal serum, which promotes the growth of fibroblasts that often overtake epithelial cell cultures.6 Although their cultures formed acinotubular-type structures when grown on basement membrane extract (BME), the murine-derived BME cannot be used for tissue engineering in humans.6
Our study delineates a human-compatible system for the differentiation of human salivary gland cells into functional salivary units. To differentiate, cells require cues from several factors, including their extracellular matrix (ECM), growth factors, and integrin-mediated cell–cell interactions.7 In the past, basement membrane proteins used as substratum were found to be vital to the growth and differentiation of secretory cells, including mammary gland epithelial cells.8 The basement membrane is typically composed of collagen type IV, perlecan, laminin, and nidogen/entactin.9,10 Perlecan/heparan sulfate proteoglycan 2 (HSPG2), one of the critical components of the basement membrane, is a multidomain proteoglycan that forms functional attachments to multiple ECM components. Domain IV of perlecan (PlnDIV) contains a novel peptide sequence, which supports adhesion, spreading, and focal adhesion kinase (FAK) activation.11 Additionally, PlnDIV contains immunoglobulin (Ig) repeats that are similar to those found in Ig superfamily members such as the neural cell adhesion molecule or the platelet endothelial cell adhesion molecule.11
We tested the hypothesis that salivary gland cells cultured on PlnDIV peptide will receive the appropriate cues that allow them to differentiate and mimic their glandular phenotype. We used PlnDIV peptide to promote attachment and subsequent differentiation of cultured human acinar cells into salivary units, a useful first step toward the culture of acinar cells free of animal products that can be implanted into patients. These cells have the potential to polarize and differentiate into salivary units that express essential salivary biomarkers and can be used to engineer a functional artificial salivary gland.
Normal tissue specimens of the human parotid and submandibular glands were obtained from patients undergoing head and neck surgery. A consent and protocol approved by the Institutional Review Board (IRBs) of both the Christiana Care Health System (CCHS) and the University of Delaware was utilized for tissue collection. Specimens were placed on ice immediately after excision and kept cold at all times until processing in the laboratory.
Tissues were frozen and cryosectioned at a thickness of 8μm. Sections were fixed using a 1:1 mixture of acetone and methanol, stained with hematoxylin and eosin, and dehydrated by three dips each in 70%, 80%, 95%, and 100% (v/v) ethanol. The slides were mounted with Cytoseal mounting medium (Electron Microscopy Sciences, Fort Washington, PA) and coverslipped.
Tissue cryosections were fixed using an acetone and methanol mixture, and hydrated using water. The slide then was oxidized with 0.5% (v/v) periodic acid solution, rinsed with distilled water, and then treated with the Schiff reagent. Slides were rinsed, counterstained with Mayer's hematoxylin, and then rinsed again. Lastly, they were dehydrated and coverslipped using Cytoseal mounting medium.
The ductal cell isolation protocol previously reported by Tran et al. was modified to select for acinar cells.4 Freshly obtained salivary gland tissue was washed with cold F-12 medium (Invitrogen, Carlsbad, CA) supplemented with 1% (v/v) penicillin–streptomycin, 1% (v/v) amphotericin B (Invitrogen), and 1% (v/v) Betadine solution. This wash was followed by a series of F-12 medium washes without the Betadine solution. The tissue specimen then was minced into small pieces and was subjected to an enzymatic tissue digestion step. The minced tissue slurry was added to a dissociation buffer with the Liberase RI enzyme (0.2U/mL from Roche Diagnostics, Indianapolis, IN) and 0.1% (w/v) trypsin (Fisher Scientific, Pittsburgh, PA) in F-12 medium and incubated for ~1.5h at 37°C. Smaller pieces of minced tissue that dissociated faster yielded acini sooner than the larger tissue chunks. These dissociated acini were centrifuged earlier (incubation time ~1h) at a low speed (180g for 5min) and pelleted sooner to avoid over-digestion and possible destruction during the ongoing enzymatic action. The larger clumps were resuspended in the dissociation buffer until they dissociated into individual acini and in some cases into individual acinar cells. These gentler centrifugation steps and sequential digestion steps allowed for the isolation of viable acinar cells. After incubation, the buffer was centrifuged (180g for 5min) to obtain a cell pellet, which then was resuspended in serum-free Hepato-STIM™ medium (BD Biosciences Discovery Labware, Bedford, MA) or a medium substituted with human serum albumin, supplemented with 1% penicillin–streptomycin and 1% amphotericin B. The cell suspension was filtered through a 70μm cell strainer (BD Biosciences Discovery Labware). The isolated cells then were seeded into a six-well culture plate and maintained at 37°C in a humidified atmospheric chamber containing 5% (v/v) CO2. Cells were passaged with 0.05% trypsin (w/v) (Invitrogen) ethylenediaminetetraacetic acid upon confluency. The enzymatic action of trypsin was stopped by addition of trypsin soybean inhibitor (Sigma, St. Louis, MO). The cell suspension was pelleted and resuspended in medium, and cells were counted using a hemacytometer. Cells then were replated at a dilution of 1:10.
For all studies involving ECM proteins, laminin (100μg/mL) (BD Biosciences Discovery Labware), Matrigel (1:3 dilution) (BD Biosciences Discovery Labware), or PlnDIV peptide (10μg/mL) was added as a thin coating on chamber slides (eight well). PlnDIV peptide was kindly synthesized by Lisa Haines-Butterick (Chemistry and Biochemistry, University of Delaware). Laminin or PlnDIV peptide was allowed to air-dry overnight, while Matrigel was added to the chambers an hour before cell seeding. All residual Matrigel was removed until a thin film remained. Cells were seeded at equal density on each matrix. Cells in their third or fourth passage were used for all experimental analyses. Cells in these passages behaved the same way in all experiments.
Antibodies used in this study included polyclonal anti-human α-amylase (Sigma), Zonula Occludens-1 (ZO-1) (Zymed Laboratories, South San Francisco, CA), laminin (Collaborative Research in Bedford, MA), aquaporin 5 (AQP5) (Alpha Diagnostics International, San Antonio, TX), cytokeratin (CK) 14 (Gift from Dennis Roop, Baylor College of Medicine, Houston, TX), and monoclonal antibodies CK 19 (Amersham, Little Chalfont, United Kingdom), perlecan A76 (as previously described12), anti-FAK phospho Y397 (Millipore, Bedford, MA), anti-mouse fluorescein isothiocyanate–conjugated E-cadherin (BD Pharmingen, San Diego, CA), and Alexa 488–conjugated Phalloidin (Invitrogen). Alexa 488– and Alexa 568–conjugated secondary antibodies against mouse and rabbit IgG (Invitrogen) were used.
Tissue cryosections (8μm thick) of human salivary gland were fixed with cold methanol for 10min, and rehydrated with 1× phosphate-buffered solution (PBS) for 5min. Subsequently, tissue sections were blocked with 3% (w/v) bovine serum albumin in PBS for 16h at 4°C. Primary antibodies were prepared in blocking solution and incubated for 45min at 37°C in a humidified chamber. After washing with 1× PBS, secondary antibodies prepared in blocking buffer were added and incubated for 40min at 37°C. Draq5 (Biostatus, Leicestershire, United Kingdom) was subsequently added as a nuclear stain. Slides were washed, mounted, and coverslipped.
Staining with cultured human salivary gland cells grown in eight-well chamber slides (Lab-tek® Products; Nalge Nunc International, Naperville, IL) was performed as described above with minor changes. Cells were seeded on eight-well chamber slides coated with laminin (100μg/mL), Matrigel (1:3 dilution), or PlnDIV (10μg/mL) peptide. In brief, cells fixed with methanol were rehydrated with 1× PBS and permeabilized with 0.1% (v/v) Triton X-100. Cells were blocked overnight in 3% bovine serum albumin in PBS (w/v). Primary antibody was applied for 1h at 37°C in a humidified chamber. Secondary antibody was applied for 40min at 37°C followed by Draq5 addition as a nuclear stain. Negative controls were performed for both the tissue sections and cultured cells without addition of the primary antibody. Primary antibodies specific for antigens expressed by other cell types present in the same tissues or cells also served as negative controls for nonspecific binding to tissues. Cells were mounted with Gel Mount (Biomeda Corporation, Foster City, CA) and stored in the dark at 4°C before viewing with the confocal microscope.
Salivary gland cells were grown on plastic-coated, Matrigel-coated, or PlnDIV peptide–coated dishes as above. Cells were lysed with RIPA buffer (150mM NaCl, 1% NP-40, 0.5% deoxycholic acid, 0.1% sodium dodecyl sulphate, and 50mM Tris pH 8.0) containing a protease inhibitor cocktail as well as phosphatase inhibitors. Lysate was centrifuged for 10min at 13,000rpm (Eppendorf 5402 centrifuge) at 4°C, and the pellet was discarded. A BCA (bicinchoninic acid) protein estimation assay was performed to measure the concentration of protein in the supernatant. Aliquots from each sample (50μg) were separated on sodium dodecyl sulfate polyacrylamide gel electrophoresis gradient gels. Proteins then were transferred onto a nitrocellulose membrane. The membrane was incubated in 1:1000 dilution of the primary antibody, pFAK Y397 (BD Biosciences, Bedford, MA) or Anti-FAK (Millipore), followed by 1:200,000 dilution of horseradish peroxidase linked to mouse IgG. Negative controls were performed using preimmune serum and secondary antibodies. Protein bands were detected by using a chemiluminescent substrate (Thermo-Fisher, Rockford, IL) and were quantified using the Scion Imaging Software (Scion Corporation, Frederick, MD).
Culture medium from human parotid gland cells grown on plastic, Matrigel, or PlnDIV peptide was collected every 2, 4, and 6 days. Culture medium was changed every 48h. An α-amylase activity kit (Salimetrics, State College, PA) used a chromogenic substrate, 2-chloro-p-nitrophenol linked with maltose. Culture medium (50μL) was mixed with 200μL of prewarmed α-amylase substrate and color development measured at 405nm. Absorbance was measured every 5min for 1h. The change in absorbance was used to calculate amylase activity.
Human parotid gland cells (10,000/well) were cultured on plastic, laminin, Matrigel, or PlnDIV peptide. A water-soluble tetrazolium (WST) salt (Roche Molecular Biochemicals, Mannheim, Germany) was added in the cell culture plates and incubated for 1h at 37°C. The conversion of the WST salt to formazan, a colorimetric change, was measured using a plate reader at 470nm. The proliferation of cells was measured every 2, 4, and 6 days in culture.
Our goal in this study was to recreate the structure of the native salivary gland in culture and ensure that it has all the essential proteins that will aid in secretion of salivary fluid. To recreate functional salivary units, the native structure of the excised salivary glands was evaluated by hematoxylin and eosin staining. Purple-stained acini surrounded large intercalated and striated ducts (Fig. 1A). Acinar and ductal structures were identified using periodic acid Schiff staining (Fig. 1B). Stained acinar cells easily were distinguished from the unstained ductal cells. Cells were isolated from the salivary gland tissue as described above. Initially, a mixed culture of cells was obtained (Fig. 1C). The mixed culture of cells contained a majority of small epithelial cells, which had acinar-like morphology and a minority of larger cells that appeared to be of ductal origin. Cultured cells maintained through multiple passages under these conditions lost the minority of ductal cells, indicating that the culture conditions favored the growth of acinar cells. Because acinar cells are mainly responsible for secreting salivary fluid, acinar-enriched cultures (Fig. 1D) were used for further study.
Salivary gland cells were characterized by their expression of cell-type-specific biomarkers. Myoepithelial cells present in the salivary gland tissue were identified using the marker CK 14. Myoepithelial cells stained strongly and were found wrapped around the acini in the tissue (Fig. 2A). To parallel the findings in the tissue with the isolated cells, the mixed culture of isolated cells was stained with CK 14. None of the cells in the mixed culture were positive for CK 14, suggesting that no myoepithelial cells were present in our mixed culture (not shown). Further, tissue staining with the ductal cell marker, CK 19, revealed positive staining of large ductal structures (Fig. 2B). Interestingly, the large cells from mixed cell cultures also stained positively for CK 19, indicating that they were derived from the ductal structures in the gland (Fig. 2D).
α-Amylase, the predominant enzyme found in saliva that is abundantly produced and secreted by the acinar cells, was used as a marker to identify the acinar cells in the gland. Staining of salivary gland tissue sections displayed abundant expression of α-amylase in the acini structures (Fig. 2C). To parallel studies with cultured cells, the mixed population of cells was subcultured and subjected to conditions that favored the growth of acinar cells, yielding an acinar-enriched culture. α-Amylase staining for these acinar-enriched cultures was strongly positive (Fig. 2E). Because our tissue engineering approach is designed for the direct use of cultured acinar cells, rather than the re-engineering of ductal ones, we used the acinar-enriched cultures for all future analyses.
Because some self-assembly was observed in the acinar-enriched cell cultures, expression of certain candidate basement membrane components was investigated in tissue sections as well as in the cultured cells. Tissue cryosections of the salivary glands were stained with antibodies to the common ECM proteins. Immunohistochemical analyses revealed the presence of significant levels of perlecan and laminin in the basement membrane of the salivary gland tissue (Fig. 3A–C). Among the acinar-enriched cell cultures, 8 days of culturing led to robust expression of perlecan and laminin in their secretory route (Fig. 3D–F). At 12 days in culture, perlecan was secreted and organized by the acinar cell cultures (Fig. 3G, I).
Cell viability of acinar-enriched cultures grown on plastic, laminin, Matrigel, or PlnDIV peptide was evaluated using the WST assay. Cell viability and growth remained similar for cells plated on each of the matrices. No significant differences were noted in the rate of proliferation of the cells among the different matrices (Fig. 4).
The morphology of the acinar cells grown on plastic, laminin, Matrigel, or PlnDIV peptide was assessed. Cell morphology remained similar on all four of these matrices at day 1 (Fig. 5A–D). At day 6, the cultured cells showed noticeable differences. While the morphology of cells on plastic or laminin remained the same (Fig. 5E, F), the cells growing on Matrigel or PlnDIV peptide began to differentiate and self-assemble into clusters (Fig. 5G, H). At day 8, small lobules developed inside the self-assembled structures on Matrigel as well as on PlnDIV peptide (Fig. 5I, J). Large lobular structures with interconnections developed by day 15 on Matrigel (Fig. 5K) and on PlnDIV peptide (Fig. 5L).
Tissue sections and cultured cells were analyzed for the presence of tight junctional components. Both tissue sections (Fig. 6A) and the acinar-enriched cultures grown on plastic, Matrigel, or PlnDIV peptide (Fig. 6B–D) all were found to express E-cadherin at their tight junctions. Tissue sections and acinar-enriched cultures grown on Matrigel or PlnDIV peptide were stained for AQP5 to determine the presence of this critical water channel protein. The acini in the salivary gland tissue sections were observed to highly express AQP5 at their cell membranes (Fig. 7A). The ductal structures, however, did not show the presence of this water channel protein (Fig. 7B). Among the acinar cells cultured on Matrigel or PlnDIV peptide, population clusters undergoing self-assembly were positive for AQP5 (Fig. 7C).
Acinar-enriched cell cultures grown on Matrigel or PlnDIV peptide assembled stress fibers and complex filopodial structures (Fig. 8B, C) as shown at higher magnification in Figure 8F and H. In contrast, cells grown on plastic displayed extensive actin filaments along the periphery of the cells (Fig. 8A, D) consistent with the more stationary, attached behavior seen in Figure 5A and E. Z-stacks performed on the phalloidin stainings confirm that our observations were consistent on all focal planes (data not shown). Phalloidin staining (green) of cells grown on Matrigel or PlnDIV peptide revealed a dense organized network of actin stress fibers (Fig. 8F, H), indicating that cells cultured on either of these matrices are able to migrate and form higher-order lobular acinar structures such as those shown in Figure 5I and J.
Experiments with salivary gland cells plated on either Matrigel or PlnDIV peptide showed clear FAK phosphorylation on tyrosine 397 at discrete cellular sites that correspond to sites of focal adhesions (red staining, Fig. 8F–I). Cells coated on plastic displayed expression of phosphorylated FAK throughout the cytoplasm and occasionally localized it to punctate adhesion sites (Fig. 8D, E). Western blot analysis of phosphorylation at this site, however, showed no clear differences in levels of FAK activation in cells plated on PlnDIV peptide when compared to cells on plastic (Fig. 8J, K). As expected, cells cultured on Matrigel expressed robust total FAK and FAK phosphorylation, higher than either on plastic or PlnDIV peptide (not shown). Thus, although cells growing on plastic or PlnDIV peptide express similar levels of activated and total FAK, they localize it differentially with cells growing on PlnDIV peptide able to localize it to focal adhesions at sites of rapid cytoskeletal reorganization.
To better view α-amylase expression within the well-differentiated structures, acinar-enriched cultures were grown on Matrigel or PlnDIV peptide for 20 days. Z-stacks of the acini-like structures were obtained to view expression of α-amylase in optical slices through the structures. At day 20, a representative structure on Matrigel grew to approximately 86μm above the surface of the culture plate, while structures on PlnDIV peptide grew approximately 61μm. Three-dimensional reconstructions from the optical slices showed acini-like structures on Matrigel (Fig. 9A) and on PlnDIV peptide (Fig. 9B) rising from a monolayer of cells. Structures on both matrices formed tight junctions and demonstrated robust expression of α-amylase.
To demonstrate α-amylase secretion by the acinar-enriched cultures growing on PlnDIV peptide or Matrigel, α-amylase activity in their culture media was measured over a 6-day period. Activity measurements from cell cultures of both Matrigel and PlnDIV peptide gave similar values and were found to increase over time (Fig. 10).
Tissue engineering of a salivary gland requires functional cells that express the vital proteins needed to restore secretion. Here, we report a technique for isolation and maintenance of human salivary gland cells and their differentiation via a human sequence in perlecan that allows for self-assembly of these cells into functional acini-like units, suitable for tissue engineering. We envision that the salivary acinar cells will grow and differentiate, initially, in an artificial scaffold and eventually fully integrate into the patients' subcutaneous buccal mucosa or native salivary gland tissue.
To recreate the native structure of the salivary gland in vitro, biomarkers from the salivary gland tissue were compared in parallel with cells isolated from specimens of the glandular tissue. Tissue cryosections from the human parotid and submandibular glands were obtained for initial histological and immunohistochemical studies. Parallel biomarker studies with CK 19 and α-amylase revealed the presence of ductal and acinar cells, respectively, in both salivary gland tissue and primary cell cultures. Myoepithelial cells were identified in the tissue; however, negative staining for CK 14 revealed their absence from primary cell cultures. Although mixed cultures of acinar and ductal cells were isolated initially, novel culturing techniques were used to obtain acinar-enriched cultures.
The basement membrane, one of the most important components of the ECM of polarized epithelial cells, directs the growth and differentiation of human epithelia.13 In addition to separating the parenchymal cells and connective tissues, the basement membrane also is involved in encasing, organizing, and guiding the behavior of the parenchymal cells that reside on it.14 In the case of the salivary epithelium, a major function of the basement membrane is maintenance of directional secretion. Abundant expression of perlecan and laminin in the tissue reveals the importance of these proteins for maintaining the phenotype of salivary gland cells. The functionality of the cultured salivary acinar cells in vitro is confirmed by their secretion and organization of their own basement membrane including the critical component perlecan that can support further growth and differentiation by virtue of its ability to interact with cell adhesion molecules that can influence integrin-mediated events.11,15 Uniquely, no non-human-derived material was needed for isolation or culture of the acinar cells.
To aid further differentiation of salivary gland cells into organized glandular structures that express essential biomarkers, acinar cells were cultured on ECM proteins such as perlecan, laminin, and the commonly used BME, Matrigel. Because full-length perlecan cannot be produced in recombinant form because of its large size, and because we wished to avoid animal proteins, human-compatible PlnDIV peptide sequence was used in studies involving perlecan. Evaluation of the behavior of acinar cells grown on Matrigel, laminin, or PlnDIV peptide showed no differences in growth and proliferative capabilities of cells. Morphological analysis revealed that Matrigel and PlnDIV peptide supported gradual differentiation of the cells. Lobe formation with interconnections were observed in cells grown on Matrigel as well as on PlnDIV peptide. Differentiated structures self-assembled into acini-like structures that are reminiscent of salivary units.
α-Amylase, one of the major proteins in saliva that helps the breakdown of starches and initiates digestion, is highly produced and secreted by acinar cells. Differentiated acini-like structures mimicked salivary units and produced and secreted α-amylase. Additionally, α-amylase secretion from differentiated structures increased over time, as seen by amylase activity measurements, suggesting development of functional salivary units.
Tight junctions, vital components in polarized secretory epithelia, hold cells together and form a barrier to maintain transcellular transport. E-cadherin expression confirmed formation of tight junctions in acinar cell cultures grown on plastic, Matrigel, as well as PlnDIV peptide. Additional markers of polarization include AQP, which are important indicators of fluid secretion and play an essential role in saliva production. Lack of AQP5 decreased rates of salivary secretion in mice.16,17 AQP5 expression in apical membrane of acini in salivary gland tissue revealed their secretory abilities. Because epithelial cells must be polarized to secrete fluid unidirectionally, the expression of AQP5 at sites of self-assembly in cultured cells revealed their polarization and secretory potential. Additionally, recent reports have shown that activation of muscarinic acetylcholine receptors is important for translocation of AQP5 to the apical plasma membrane of salivary gland cells.18,19 Self-assembled and differentiated acinar cells expressing AQP5 might receive appropriate signals from stimulatory receptors for AQP5 translocation.
For cells to differentiate and self-assemble, they must receive appropriate signals from the ECM. Stress fiber formation and signals generated from cytoskeletal tension provide essential cues for dynamic cellular organization into higher-order structures.20 Formation of ordered F-actin stress fibers and activation of FAK also are crucial for cell migration.21 The organization of dense stress fiber networks and filopodia in acinar cells cultured on Matrigel or PlnDIV peptide demonstrated their differentiation and migratory potential. Focal adhesions are dynamic protein complexes that form connections between the cell cytoskeleton and the ECM.22 Salivary gland cells grown on Matrigel or PlnDIV peptide supported FAK activation and correctly localized it to adhesion sites, reflecting signal transduction events between the ECM proteins and the cytoskeleton. FAK staining was observed in the cytoplasm of cells grown on plastic, but not many punctate focal adhesions were seen. Thus, although these cells cultured on plastic are expressing some basement membrane proteins on their own, they evidently lack contextual information required to consistently localize FAK to sites of integrin clustering. In contrast, the redistribution of phospho-FAK seen on Matrigel and PlnDIV peptide to the ends of stress fiber assemblies on filopodia can support the organized differentiation of salivary acinar cells.
Information provided by the PlnDIV peptide accelerated the self-assembly of salivary gland cells into lobular structures better than did the intact perlecan produced by the cells themselves. The intact perlecan molecule that is produced by cultured acinar cells is expected to be modified by heparan sulfate chains that in previous studies were found to conceal the adhesive domain IV motifs.11 Fibroblasts cultured on intact perlecan failed to make stable focal adhesion contacts because of the antiadhesive activity of gagosylated perlecan domain I.23 Consistent with this, our studies showed less FAK activation and, more strikingly, a failure to cluster phosphorylated FAK to sites of focal adhesions when cells were cultured on plastic. The mechanism by which PlnDIV peptide triggers self-assembly of salivary gland cells remains unknown. Our earlier studies suggest that adhesion to PlnDIV peptide is mediated through nonintegrin receptors as inhibition of β1 integrins only partially affected adhesion to surfaces modified with the peptide.11 The peptide's origin from an Ig loop on PlnDIV suggests that signaling could be induced via interactions with Ig superfamily members on the surfaces of the acinar cells through homophilic or heterophilic binding events. These interactions can initiate signaling events that alter cytoskeletal dynamics and organization leading to cell migration, differentiation, and ultimately acinar assembly.24
Prior reports of cell transplantation into animal models resulted in regeneration of ductal cells but lacked acinar cell differentiation.25 The cells characterized in this study have the potential to be functional in an artificial scaffold that provides them the appropriate conditions to differentiate. Efforts to devise a three-dimensional culture system with a biomaterial scaffold consisting of PlnDIV peptide are underway. The culture system consisting of PlnDIV peptide reported here will aid the development of an artificial salivary gland that will foster formation of functional salivary units capable of secreting salivary fluid and that can be implanted into patients to relieve xerostomia.
This work was supported by private philanthropic contribution and NIH/NCI P01-CA098912 and COBRE P20-RR016458 (to M.C.F.-C). The authors thank Dr. Randall Duncan and Dr. Ken van Golen for providing their expert advice on cytoskeletal studies.
No competing financial interests exist.