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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
J Neurosci. Author manuscript; available in PMC 2010 April 7.
Published in final edited form as:
PMCID: PMC2791207
NIHMSID: NIHMS157889

The spatial patterning of mouse cone opsin expression is regulated by BMP signaling through downstream effector COUP-TF nuclear receptors

Abstract

Cone photopigments, known as opsins, are pivotal elements and the first detection module employed in color vision. In mice, cone photoreceptors are distributed throughout the retina, and S- and M-opsins have unique expression patterns in the retina with a gradient along the dorsoventral axis; however, the mechanisms regulating the spatial patterning of cone opsin expression have not been well documented. The purpose of this study was to define the mechanisms regulating the spatial patterning of cone opsin expression. By analyzing knockouts for bone morphogenetic protein (BMP) signaling, we found an essential role for BMP in forming cone opsin expression patterns in the retina; however, BMP signaling is activated only transiently in the dorsal half of the retina during early retinal development. Thus, BMP is not likely to play a direct role in opsin gene expression, which starts at a later stage of retinal development. We identified the chicken ovalbumin upstream promoter-transcription factor (COUP-TF) nuclear receptor as a link between BMP and opsin expression. BMP signaling is essential for the correct dorsoventral spatial expression of COUP-TFI and -TFII. Through gain- and loss-of-function analyses, we found that both COUP-TFI and -TFII are required to suppress S-opsin expression in the dorsal retina but that only COUP-TFI plays an essential role in suppressing M-opsin expression in the ventral retina. Based on these findings, we propose a new molecular cascade involving BMP and COUP-TFs that conveys dorsoventral information to direct the expression of cone opsins during retinal development.

Keywords: BMP signal, mouse retina, nuclear receptor, S-opsin, M-opsin, photoreceptor

Introduction

Cone photopigments, known as cone opsins, are pivotal elements and the first detection module employed in color vision. Several different cone opsins exist, each with a distinct wavelength sensitivity (Dartnall et al., 1983). In vertebrates, the spatial expression pattern of cone opsins across the retina defines the retinal field (the fovea in primates, the visual streak in chickens, or the simple dorsal-ventral and nasal-temporal gradients in fish and rodents) (Bruhn and Cepko, 1996; Szel et al., 1996; Takechi and Kawamura, 2005). However, the mechanisms that regulate the expression patterns of these cone opsins during establishment of the retinal field remain poorly understood. In mice, short-wavelength (S)- and middle-wavelength (M)-opsins have unique expression patterns in the retina, with gradients along the dorsoventral axis —although cone photoreceptors are distributed throughout the entire retina (Applebury et al., 2000).

The molecular players involved in establishing such spatial expression patterns have been reported and include several opsin expression-specific transcription factors, such as thyroid hormone receptor β2 (TRβ2), retinoid × receptor γ (RXRγ), and retinoid-related orphan receptor β (RORβ) (Ng et al., 2001; Roberts et al., 2005; Srinivas et al., 2006). However, since these transcription factors are expressed uniformly across the retina, other factors expressed in either the dorsal or ventral retina may regulate the establishment of mouse cone opsin expression patterns.

During early embryogenesis, secreted factors such as the dorsally localized bone morphogenic protein-4 (BMP4) and the ventrally localized sonic hedgehog within the optic cup appear to control dorsoventral axis patterning in the developing retina (Jean et al., 1998; Koshiba-Takeuchi et al., 2000; Zhang and Yang, 2001; Murali et al., 2005). Dorsal BMP signaling controls retinal ganglion cell axon projection through establishment of the dorsoventral expression pattern of related genes in the retina; however, the involvement of BMP signaling in later retinal differentiation has not been reported.

Nuclear receptors, a large family of ligand-dependent transcription factors, have been shown to be involved in gene networks during embryonic development and in adult physiology. In this study, we focused on chicken ovalbumin upstream promoter transcription factors (COUP-TFs), which are highly conserved members of the steroid/thyroid hormone receptor superfamily. Mice have two COUP-TF homologs, COUP-TFI and -TFII, with an exceptionally high degree of homology at the amino acid level (Qiu et al., 1994; Tsai and Tsai, 1997). COUP-TFs are expressed in several organs during embryonic development. COUP-TFI-null mice die perinatally from starvation and dehydration and exhibit defects in neuronal development and axon guidance (Qiu et al., 1997). In contrast, COUP-TFII-null mutants exhibit defects in angiogenesis and heart development and die before embryonic day (E) 10.5 (Pereira et al., 1999). To circumvent this early embryonic lethality, tissue-specific ablation of COUP-TFII has been attempted in several organs (Lee et al., 2004; Bardoux et al., 2005) but not in ocular tissue. The importance of COUP-TFs in retinogenesis was demonstrated initially in Drosophila (Mlodzik et al., 1990). Zebrafish COUP-TF homologs svp[40] and svp[44] are also known to be expressed in the retina (Fjose et al., 1993). However, the role of COUP-TFs in retinal development in vertebrates has not been demonstrated.

Materials and Methods

Mice and Rat

ICR mice and Wister rats were obtained from Japan SLC Co (Hamamatsu, Japan). Instructions for generating retina-specific BMP receptor knockout mice, BMPr1a−/fx;BMPr1b+−/ ;Six3-Cre (BMPR CKO), have been described previously (Murali et al., 2005). For controls, we used retinas from BMPr1a+/fx;BMPr1b+/−;Six3-Cre mice. We maintained these mice in a mixed 129-C57BL/6 background. COUP-TFI-null mice have been described previously (Qiu et al., 1997). In this study, COUP-TFIIfx/fx;Rx-Cre mice are referred to as retina-specific COUP-TFII-null mice (K. Tang, S.Y. Tsai and M-J Tsai, in preparation). For this study, we defined the day that the vaginal plug was detected as embryonic day 0 (E0), and we defined the day of birth as postnatal day 0 (P0). All animal experiments were approved by the animal care committees of the Institute of Medical Science, University of Tokyo; Baylor College of Medicine; and The University of Texas M.D. Anderson Cancer Center.

Cell lines and retinal explant culture

We obtained Y79 cells (Reid et al., 1974) from the Riken Cell Bank (no. RCB1645) and cultured them in RPMI 1640 medium (Nakarai, Kyoto, Japan) supplemented with 10% fetal calf serum. COS7 cells were grown in DMEM medium (Nakarai) supplemented with 10% fetal calf serum. We used a mouse retinal explant culture, which was suitable for testing the effect of environmental tissues on the neural retina because all relevant tissues (e.g., retinal pigment epithelium [RPE], ciliary body [CB], iris, stroma, and cornea) were removed. Retinal explant cultures were prepared as described previously (Tabata et al., 2004). Briefly, retinas derived from mouse embryos, in which dorsoventral orientations were marked by making small incision, were placed on a Millicell chamber filter insert (Millipore, Bedford, MA). Filters were placed into a six-well plate containing 1 ml of explant media, comprising 50% MEM (Invitrogen, Carlsbad, CA), 12.5 μM Hepes (Invitrogen), 25% Hank’s solution (-) (Invitrogen), 25 % horse serum (JRH Biosciences, Lenexa, KS), 5.75 mg/ml glucose, 200 μM L-glutamine, and penicillin/streptomycin (Nakarai), and cultured.

Immunostaining

Immunostaining of frozen-sectioned eyes or retina was done as described previously (Tabata et al., 2004) using the following primary antibodies: mouse monoclonal antibodies against COUP-TFI (1:500, PPMX, Tokyo, Japan) and COUP-TFII (1:500, PPMX), and rabbit polyclonal antibodies against S-opsin (1:2000, Millipore), M-opsin (1:2000, Millipore), and mCAR (1:2000, kind gift from Dr. CM Craft, The Keck School of Medicine of the University of Southern California). We visualized the binding of primary antibodies by using appropriate secondary antibodies conjugated to Alexa-488 or Alexa-594 (1:1000, Invitrogen). Whole-mount immunostaining of retinal explant was done with the same protocols of sections, except for the presence of 0.1% of TritonX-100. The samples were mounted in 50% glycerol and analyzed using a Zeiss Axioplan (Carl Zeiss).

Quantifying opsin- and mCAR-positive cells in frozen-sectioned eyes

To quantify the number of opsin- and mCAR-positive cells in dorsal- and ventral-region of the retina, we used opsin- or mCAR-immunostained frozen sections of meridian line. Under the binocular, we counted positive cells in the region up to 350 μm from the tip of the dorsal and ventral peripheries of the retina. We examined retinas from at least three BMPR CKO and control mice.

Plasmids

cDNAs encoding mouse COUP-TFI-, COUP-TFII, RORβ2-, BMP4-, and Tbx5-open reading frames (ORFs) were isolated by reverse transcription-polymerase chain reaction (RT-PCR) using mouse retinal RNA, and then cloned into pGEM-T-Easy vector (Promega, Madison, WI). Expression vectors containing COUP-TFI (pcDNA/COUP-TFI), COUP-TFII (pcDNA/COUP-TFII), RORβ2 (pcDNA/RORβ2), BMP4 (pcDNA/BMP4), and Tbx5 (pcDNA/Tbx5) cDNA were made by inserting the ORF into the Not I site of pcDNA3.1(+) (Invitrogen). pcDNA3/FLAG-Crx was kindly provided by Drs. Y. Yanagi and S. Kato (University of Tokyo). Constitutively active type I BMP receptor-expression plasmids, pcDNA/ALK3(QD) and pcDNA/ALK6(QD), were kindly provided by Drs. T. Imamura (The JFCR Cancer Institute) and K. Miyazono (University of Tokyo). To construct S-opsin-luc and rhodopsin-luc reporters, DNA fragments of mouse S-opsin- and mouse rhodopsin-promoter/enhancers, which contain 523 bp and 107 bp upstream region from ATG start codon, respectively, were cloned from mouse genomic DNA by PCR. Then, the fragments were inserted into the pGL3 basic vector (Promega). To construct S-opsinΔ-luc reporter, which has internal deletion from 251 to 286 of S-opsin-promoter/enhancer (supplemental Fig. S5A), internally deleted fragment of S-opsin promoter/enhancer was obtained by PCR-mediated mutagenesis and cloned into the pGL3 basic vector (Promega). To construct shRNA vectors, a target sequence of human COUP-TFII corresponding to nucleotide positions 1018–1040 (5′-AAGTCTCAGTGTGCTTTGGAAGA-3′) was selected using siDirect (http://design.rnai.jp/), and that of human COUP-TFI corresponding with nucleotide positions 321–343 (5′-AAGTTTCTTCAAGAGGAGCGTCC-3′) was selected using Gene Link RNAi Explorer. BLAST analysis showed that no homology existed between each target sequence and any other sequence in the human genome database. For shRNA expression, a double-stranded oligonucleotide covering shRNA sequences was subcloned as described previously (Lin et al., 2008) using pU6 shRNA expression vector (Yamamichi et al., 2005).

Luciferase reporter assay

GeneJuice (Novagen, San Diego, CA) was used to transfect cells in 12-well dishes with 100 ng of reporter and 300 ng of expression plasmids and/or shRNA-expressing plasmids as indicated in the Figures. Total amount of plasmid DNA was adjusted to 1 μg by adding pcDNA3.1(+) empty vector (Invitrogen). Cells were harvested at 24 h (COS7) and 48 h (Y79) after transfection, and luciferase activity was examined by a luminometer. Values were expressed as relative luciferase activities normalized to protein concentration. Experiments were performed in triplicate, and all assays were repeated at least two times.

Semi-quantitative RT-PCR

Total RNA was purified from Y79 cells transfected with expression plasmids using TRIZOL reagent (Invitrogen), and cDNA was synthesized using Superscript II (Invitrogen). All primer sets were tested using different numbers of cycles (18–34 cycles) with rTaq (Takara, Shiga, Japan), and the semiquantitative cycle number was determined for each primer set. PCR products were visualized with ethidium bromide.

Quantitative PCR

Levels of G3PDH, L-opsin, M-opsin, COUP-TFI and COUP-TFII mRNA were measured by SYBR Green-based quantitative PCR (qPCR), using cDNA prepared from Y79 transfectants. In the case of that of S-opsin, Hydrolysis probe-based qPCR assays, conventionally called TaqMan assays, was performed using #18 probe from the universal probe library (Roches, Indianapolis, IN). All qPCR analysis was performed using the Roche LightCycler 1.5 apparatus and analyzed by the Second Derivative Maximum Method for quantification (Roche).

Fluorescence-activated cell sorting (FACS)

Y79 cells were transfected with shRNA-expression plasmids together with pCS2+/EGFP, which was used to mark transfected cells. After 48 h, EGFP-positive cells were sorted by FACSAria (BD Biosciences) and subjected to RT-PCR for monitoring the level of gene expression in the transfected cells.

Results

Topographical information during early embryogenesis determines opsin expression pattern

During early retinal development, the dorsal and ventral hemispheres of the retina are asymmetrically formed when the optic vesicle undergoes active invagination to form a goblet-shaped optic cup, suggesting that the dorsal and ventral halves of the retina attach differently to adjacent tissues. Therefore, we hypothesized that this interaction creates an asymmetrical environment in the dorsal or ventral hemisphere of the retina to establish unique opsin expression patterns. To test whether environmental tissues of retina are required to establish the spatial patterns of cone opsin expression, we analyzed S- and M-opsin expression in the mouse retina cultivated by a retinal explant culture. In this type of culture, mouse retina at the embryonic stage differentiates into a three-layered structure. The mature retina of ICR mice exhibited a dorsoventral gradient of opsin gene expression, which was also observed in C57Bl/6 mice (Supplemental Fig. S1). First we examined whether S- and M-opsins are expressed properly in a retinal explant culture prepared from an E17 ICR mouse. S-opsin was expressed in the ventral half after 14 days of culture (Fig. 1, left column, second panel from the bottom), which was consistent with previous results from the P0 retina (Wikler et al., 1996). Although previous studies failed to observe M-opsin expression in retinal explant cultures (Soderpalm et al., 1994; Wikler et al., 1996), we observed M-opsin expression with a clear dorsal-to-ventral gradient as seen in vivo (Applebury et al., 2000) (Fig. 1, right column, second panel from the bottom). Next we examined the time course of S- and M-opsin expression. S- and M-opsins were first detected after 3 and 7 days of culture, respectively, which is consistent with the in vivo observation that S-opsin expression starts earlier than M-opsin expression. The experiment was then repeated using a retinal explant prepared from an E12 retina. Again, a unique dorsoventral gradient of S- and M-opsin expression was established during culture (Fig. 1, bottom panels). These results suggest that positional information to regulate opsin expression is imposed by E12 and is maintained intrinsically in the retina during development.

Figure 1
Opsin expression patterns in retinal explant cultures. Retinal explants prepared from embryonic day E17 or E12 mice were cultured for the indicated number of days. Whole-mount immunostaining was performed for S- and M-opsin expression, and the signals ...

BMP signaling regulates expression of opsin in dorsal cones

As dorsally localized BMP signaling is known to control retinal dorsoventral patterning, we hypothesized that determination of opsin expression patterns is also under the control of BMP signaling in the early developing retina. To test this possibility, we used retina-specific BMPR-knockout (BMPR CKO, BMPr1a−/fx;BMPr1b+/−;Six3-Cre) mice and control littermates (BMPr1a+/fx;BMPr1b+/−;Six3-Cre) (Murali et al., 2005). BMPR CKO is a heterozygous knockout of BMPR 1a and 1b, with the other allele of BMPR 1a deleted by Six3 promoter-driven Cre recombinase, which begins to exhibit activity in the retina at E9.0–9.5 (Furuta et al., 2000). High levels of phosphorylated Smad1/5/8, which directly reflects active BMP signaling, were observed in the dorsal retinas of wild-type mice but not of BMPR-CKO mice at E11.5, suggesting that BMPr1a is lost between E9.0 and E11.5 in the BMPR-CKO mouse retina (Murali et al., 2005). BMPR CKO mice exhibit characteristic defects in retinal dorsalventral polarity without overt morphological defects in the eye (Murali et al., 2005). We examined cone opsin expression pattern in BMPR CKO mice at the adult stage by immunostaining using frozen-sectioned eyes. In the retina of the control mice, the number of cones expressing S-opsin in the dorsal periphery was about half that in the ventral periphery (Fig. 2A, lower-left panels, and Fig. 2C); the intensity and number of M-opsin-expressing cells in the dorsal area was higher than that in the ventral area (Fig. 2B, lower-left panels, and Fig. 2D). In BMPR CKO mice, S-opsin expression in dorsal cones increased to roughly the level observed in ventral cones (Fig. 2A, lower-right panels, and Fig. 2C), while both the number of cells expressing M-opsin and the level of M-opsin expression decreased (Fig. 2B, lower-right panels, and Fig. 2D). The number of cone cells, indicated by the number of cone arrestin (mCAR)/pan-cone cell marker-positive cells, did not differ between the control and BMPR CKO mice in the dorsal and ventral peripheries (Fig. 2E). These data suggest that the attenuation of BMP signaling in BMPR CKO mice causes S- and M-opsins to be expressed improperly in the dorsal cone cells without affecting the number of cone cells.

Figure 2
S- and M-opsin expression in BMPR CKO (BMPr1a−/fx;BMPr1b+/−;Six3-Cre) mouse retina. (A, B) Frozen-sectioned eyes from adult control or BMPR CKO mice were immunostained with anti-S-opsin (A) and anti-M-opsin (B) antibodies; the signals ...

Dorsoventral expression patterns of COUP-TFI and -TFII

BMP signaling is transiently activated in the dorsal retina at approximately E11, whereas expression of the S- and M-opsin genes begins at approximately E13 and P6, respectively (Fei, 2003; Applebury et al., 2007). Therefore, intermediate molecule(s) that mediate BMP signaling may serve to establish the positional information necessary for directing opsin expression at a later stage. We focused on the orphan nuclear receptors COUP-TFI and -TFII as candidate downstream effectors of dorsal BMP signaling, because dorsoventral expression of these factors was observed in the retina. We examined the detailed expression patterns of COUP-TFs by immunostaining (Fig. 3). COUP-TFI expression was detected throughout the optic vesicle at E9, but the intensity was slightly higher in the ventral half of the optic vesicle (Fig. 3A, left panel, arrows). At E11 and E14, strong expression of COUP-TFI was maintained in the ventral half of the retina, whereas its expression levels in the dorsal half were markedly lower (Fig. 3A). After birth, retinal expression of COUP-TFI was maintained throughout the ganglion cell layer (GCL) and the inner nuclear layer (INL); we also observed a dorsoventral expression gradient but with a weak incline.

Figure 3
Expression of COUP-TFI and -TFII during mouse retinal development. (A, B) Frozen-sectioned eyes from wild-type mice at the indicated developmental stage were immunostained with anti-COUP-TFI and -COUP-TFII antibodies (green). The nuclei were stained with ...

In sharp contrast to COUP-TFI, the distal region on the dorsal side of the optic vesicle strongly expressed COUP-TFII (Fig. 3B, left panel, red arrows) and presumptive RPE (Fig. 3B, left panel, arrows) at E9. We observed COUP-TFII was expressed exclusively at E11 in the dorsal area, which is consistent with previous data (McCaffery et al., 1999), and that this expression persisted until adulthood (Fig. 3B). In the INL, we observed scattered cells expressing strong COUP-TFII at E14, and these cells became restricted to the INL as the retina matured (Fig. 3B, arrowheads). Double immunostaining for several retinal subtype markers, such as HuC/HuD and glycine transporter 1, suggested that the positive cells were amacrine cells (data not shown). The third member of the COUP-TF family, COUP-TFγ (COUP-TFIII) (Jonk et al., 1994), is evolutionarily distant from COUP-TFI and -TFII, which exhibit exceptionally high homology; in contrast, the primary structures of COUP-TFγ and COUP-TFI are only 60% identical. RT-PCR experiments revealed expression of COUP-TFγ mRNA in developing mouse retina, but immunostaining experiments failed to reveal COUP-TFγ protein expression in embryonic retina (data not shown). Results of additional studies also suggest that COUP-TFγ plays a role distinct from those of COUP-TFI and -II in retinal development. Summaries of the COUP-TFI and -TFII expression patterns are given in Figure 3C and 3D.

Perturbed dorsoventral expression patterns of COUP-TFI and -TFII in BMPR CKO mouse retina

We examined whether BMP signaling regulates the unique dorsoventral expression patterns of COUP-TFI and -TFII in the retina. We used immunostaining to examine COUP-TFs expression in the retina of E14 BMPR CKO mice (Fig. 4). COUP-TFI expression in the dorsal region was enhanced in BMPR CKO mice; however, on the ventral side, no apparent difference was observed with the control littermate retina (Fig. 4A,B). In contrast, we found that COUP-TFII expression on the dorsal side was completely abolished in BMPR CKO mice (Fig. 4C, D), whereas strong amacrine cell staining in the central region of the retina was present in both BMPR CKO and control animals (Fig. 4C, arrowheads).

Figure 4
Expression of COUP-TFs in BMPR CKO mouse retina. (A-D) Frozen-sections of control and BMPR CKO mouse eyes at E14.5 were immunostained with anti-COUP-TFI (A, B) and -COUP-TFII (C, D) antibodies. The panels in the left-most column show nuclear staining ...

Altered expression of cone opsin in COUP-TF-null mouse retina

Based on these results, we hypothesized that BMP signaling regulates the expression of opsins by regulating COUP-TF expression, at least on the dorsal side. Thus, we next used COUP-TFI-null (Qiu et al., 1997) and retina-specific COUP-TFII-null mice (COUP-TFII fx/fx:Rx-Cre) to examine if COUP-TFI and -TFII are required for normal expression pattern of opsins in vivo (Fig. 5A, B). Immunostaining revealed that the expression of both S- and M-opsin was severely perturbed in the COUP-TFI-null mice. COUP-TFI-null mice expressed S-opsin at nearly equal levels on the dorsal and ventral sides of the retina, while the expression of M-opsin was up-regulated on the ventral side, resulting in loss of expression gradient on the dorsoventral axis. The retina-specific COUP-TFII-null mice expressed upregulated S-opsin on the dorsal side, although the expression was not as strong as it was in the COUP-TFI-null mice; these mice expressed a normal M-opsin pattern. These data indicated that COUP-TFI and -TFII are necessary to suppress S-opsin in the dorsal cones, whereas only COUP-TFI is required to suppress M-opsin in the ventral cones.

Figure 5
Expression of S- and M-opsin in COUP-TFI-null and retinal-specific COUP-TFII-null mouse retina. (A, B) S- (A) and M-opsin (B) expression was examined by immunostaining using frozen-sectioned eyes from COUP-TFI knockout mice (COUPI KO) at P23 and COUP-TFII ...

COUP-TFs suppress S-opsin

To gain more mechanistic insights into transcriptional control of cone opsin genes, we performed a series of experiments using the Y79 human retinoblastoma and COS7 monkey fibroblast cell lines. The 0.5-kb mouse S-opsin enhancer/promoter, which is activated by specific Crx and RORβ2 binding (Srinivas et al., 2006), was conjugated with luciferase (S-opsin-luc). S-opsin-luc was weakly activated by the expression of Crx or RORβ2; moreover, we observed a synergistic effect in Y79 cells (Fig. 6A) and COS7 cells (supplemental Fig. S2). COUP-TFI or -TFII strongly suppressed luciferase activity induced by Crx and RORβ2. COUP-TFI or -TFII suppressed Crx-induced luciferase activity (Fig. 6B, arrows) but slightly enhanced the activity induced by RORβ2 (Fig. 6B, arrowheads). We confirmed that forced COUP-TF expression did not decrease the level of Crx or RORβ2 expression by western blotting (supplemental Fig. S2). The COUP-TFs did not suppress Crx- and Nrl-induced luciferase activity driven by the mouse rhodopsin enhancer/promoter, which contains a 107-bp upstream region (Chen et al., 1997) (Fig. 6C). These results suggest that COUP-TFs specifically suppress S-opsin at the level of transcription within the S-opsin promoter, probably through the suppression of Crx activity. Moreover, the suppressive effect depends on the promoter context, based on our observation that neither COUP-TF suppressed the activity of the rhodopsin promoter.

Figure 6
Effects of changes in COUP-TF expression on opsin expression. (A-C) Transient reporter assays were performed using Y79 (A, C) and COS7 (B) cells. The cells were transiently transfected with plasmids as indicated in the Figure and cultured for 24 h (COS7) ...

Y79 is a human retinoblastoma cell line, and forced Crx expression induces S-opsin (human blue opsin) (Peng and Chen, 2007). Therefore we examined how COUP-TFs affect human S-opsin expression by looking at the expression of endogenous S-opsin mRNA via semiquantitative and quantitative RT-PCR. As we expected, Crx increased the level of endogenous S-opsin expression in Y79 cells; however, the effect was abolished by COUP-TFI or -TFII (Fig. 6D).

Knockdown of COUP-TF augmented S-opsin

We examined the effects of the shRNA-mediated knockdown of COUP-TFI or -TFII on S-opsin expression in Y79 cells. The expression of shRNAs against either human COUP-TFI (shCOUPI) or COUP-TFII (shCOUPII) effectively and specifically suppressed the endogenous mRNA expression of COUP-TFI or -TFII, respectively (Fig. 6E). In Y79 cells, Crx-induced S-opsin was enhanced by the knockdown of either COUP-TFI or -TFII (Fig. 6E), confirming that both COUP-TFs have suppressive effects on S-opsin.

Next we examined whether the basal level of S-opsin expression is regulated by endogenous COUP-TFI or -TFII in the absence of exogenous Crx. Unfortunately, we were unable to draw any conclusions from the results shown in Figure 6E because not all of the cells were transfected with the shRNA construct. Therefore, we used cells cotransfected with an EGFP expression plasmid to purify the shRNA-transfected cells by cell sorting. In this way, we detected clear augmentation of S-opsin after treating with shRNAs for COUP-TFI or -TFII (Fig. 6F), suggesting that COUP-TFI and -TFII also regulate the basal level of S-opsin.

Finally, we analyzed the effect of BMP signaling on endogenous L-opsin, M-opsin, and COUP-TFI in Y79 cells (Fig. 6G). Humans have a third opsin gene (L-opsin) that arose through duplication, resulting in a tandem array of L-/M-opsin genes on the X chromosome. BMP4 enhanced L- and M-opsin but suppressed COUP-TFI. These results are consistent with our in vivo data showing that BMP signaling had negative and positive effects on the expression of COUP-TFI and M-opsin, respectively.

Discussion

Initially we thought that the unique dorsoventral expression patterns of S- and M-opsin were achieved through their interaction with the surrounding tissues during development. However, we found that isolated retinas could establish the correct spatial expression patterns for S- and M-opsin in vitro even at E12, suggesting that the positional information required for proper opsin expression is established prior to E12, and that the surrounding tissues of the retina are dispensable in maintaining the spatial opsin expression pattern.

We found that BMP signaling, while essential for opsin expression patterning, is not the only signal that regulats opsin expression; we observed that opsin expression in BMPR CKO mice was perturbed but not abolished. Although researchers have thoroughly characterized retinal projections are regulated along the dorsoventral axis by BMP in the early stages of retinal development (Koshiba-Takeuchi et al., 2000; Trousse et al., 2001; Murali et al., 2005), little is known about the role of BMP in the later stages of retinal development, especially in retinal cell-type specification. The activation of Smad1/5/8 in the dorsal retinas of BMPR CKO mice is lost at E11.5 (Murali et al., 2005). We detected alterations in the expression of COUP-TF and opsin in Smad4 retina-specific knockout mice (data not shown), supporting the notion that canonical BMP signaling through Smad is important for COUP-TF and opsin regulation. Tbx5 is a major downstream target of BMP signaling that leads to the activation and repression of dorsal- and ventral-specific genes, respectively (Koshiba-Takeuchi et al., 2000). However, we did not detect the modulation of COUP-TF expression in response to Tbx5 (supplemental Fig. S3A), leading us to speculate that BMP uses a Tbx5-independent signaling cascade to regulate COUP-TFs expression. BMPR1b, one of the type I BMP receptors, is expressed exclusively in the ventral retina during the embryonic period (Liu et al., 2003). In BMPR1b-knockout mice, the axon pathfinding of ventrally localized retinal ganglion cells is perturbed, whereas the expression pattern of both cone opsins appears to be normal (Liu et al., 2003); this research indicates that ventral BMP signaling through BMPR1b may not significantly affect cone opsin patterning.

COUP-TFs are expressed as early as E9, which is almost the same stage at which BMP4 expression begins (Furuta and Hogan, 1998), suggesting that BMP directly affects COUP-TF expression. Although BMPR CKO mice showed a loss of COUP-TFII expression in the dorsal retina, activating BMP-related signaling in response to the expression of a constitutively active form of BMPR1a or BMPR1b, or adding recombinant BMP4 into Y79 or retinal explants did not stimulate COUP-TFII expression (supplemental Fig. S3B, C). We observed that COUP-TFI was suppressed only when BMP4 was expressed in Y79 cells. Therefore, BMP signaling is essential but may not be sufficient for the induction of COUP-TFII expression. Alternatively, BMP may act in a stage- and/or cell-specific manner or may simply maintain the expression pattern of COUP-TFII without affecting the initiation of transcription. The latter notion is supported by the observed divergent responses of COUP-TFI and -TFII to the loss of BMP signaling. Currently we are investigating the mechanism how BMP signaling regulates the expression of COUP-TFs using the isolated promoter regions of COUP-TFI and -TFII.

Retinoids stimulate the expression of COUP-TFI in the WERI-Rb1 human retinoblastoma cell line (Li et al., 2003), and we also found adding retinoic acid to Y79 cells induced COUP-TFI but not COUP-TFII (supplemental Fig. S4A). The retinoic acid signaling pathway is involved in retinal dorsoventral patterning (Marsh-Armstrong et al., 1994; Hyatt et al., 1996; McCaffery et al., 1999), and retinoic acid synthetases, such as Raldh1 and Raldh3, are differentially distributed along the dorsoventral axis of the retina (Li et al., 2000; Mic et al., 2000). Therefore, ventral retinoic acid may positively regulate COUP-TFI expression.

The nonoverlapping and temporally distinct expression patterns of COUP-TFI and -TFII in various organs during development (Lu et al., 1994; Qiu et al., 1994; Tang et al., 2005; Suh et al., 2006) suggest that the expression of COUP-TFI and -TFII is regulated independently. However, the complementary expression patterns of COUP-TFI and -TFII in the retina suggest reciprocal suppressive regulation of their expression. Indeed, the downregulation of COUP-TFII in Y79 cells led to a slight enhancement in COUP-TFI expression, supporting this notion; COUP-TFII was not enhanced following the downregulation of COUP-TFI. Furthermore, overexpression of COUP-TFI or -TFII did not alter the level of COUP-TFII or -TFI, respectively (supplemental Fig. 4B). Because complementary COUP-TFI and -TFII expression in the retina was observed only at the embryonic stage, such reciprocal regulation may be a retina stage-specific phenomenon.

When S-opsin expression began at approximately E13, weak COUP-TFI expression and strong COUP-TFII expression were observed on the dorsal side of the retina. The overexpression of COUP-TFI or -TFII indicated that they are capable of suppressing S-opsin, and our results in COUP-TFI/-TFII-knockout mice indicate that both COUP-TFI and -TFII are essential to suppress S-opsin (Fig. 7A). Therefore, we surmise that the lack of COUP-TFII expression in the dorsal retinas of BMPR CKO mice resulted in increased S-opsin expression in the dorsal cone cells, even though COUP-TFI expression increased. Because COUP-TFI ceases in the cells of the ONL after birth, the cooperative suppression of S-opsin by COUP-TFs may not be involved in maintaining the suppression of S-opsin in dorsal cone cells. TRβ2 and RXRγ are expressed in postmitotic cone cells, and the enhanced expression of S-opsin in the dorsal retina was observed in TRβ2- and RXRγ-knockout mice (Ng et al., 2001; Roberts et al., 2006). However, both proteins are uniformly expressed in the retina, so it is unclear how they suppress S-opsin only on the dorsal side. Because COUP-TFI and -TFII expression began earlier than TRβ2 and RXRγ expression, COUP-TFs may determine opsin expression pattern and TRβ2 and RXRγ may directly or indirectly maintain the dorsal suppression of S-opsin (Fig. 7B). Our analysis of COUP-TFI and -TFII expression in the retina at an early developmental stage shows that expression is not restricted to cone or cone progenitor cells. We do not think that the expression of COUP-TFs is limited to cells of the cone lineage, because COUP-TFs might function only when opsin gene activation modules are present.

Figure 7
Model of the regulation of opsin spatial expression in mice. (A) Summary of opsin and COUP-TF expression in the mutant mice used in this study. The depth of each color indicates the intensity of expression for each gene. ND, not determined. (B) Schematic ...

Recently, COUP-TFI and -TFII were shown to function cooperatively in neural stem/progenitor cells for the acquisition of gliogenic competence (Naka et al., 2008). We observed the unique expression of COUP-TFs in certain retinal cell subtypes in the INL during late development, suggesting that COUP-TFs play cooperative roles in determining the fate of retinal progenitor cells in the retina.

COUP-TFs have been reported to act as transcriptional repressors, either by recruiting a co-repressor such as N-CoR or SMRT, or by competing for DNA binding with a transcriptional activator (Tsai and Tsai, 1997). In the latter case, a putative binding site for COUP-TF has been reported (Tsai and Tsai, 1997), and we found putative binding sites for COUP-TF within the S-opsin enhancer used in our study; however, the deletion of these sites did not affect the suppressive effect of COUP-TFs (supplemental Fig. S5). On the other hand, opsin gene regulation by COUP-TFs via epigenetic effects is feasible. In fact, DNA methyltransferase 3a and 3b were identified as interacting partners of COUP-TFI, and their role in the regulation of target genes by COUP-TFI has been suggested previously (Gallais et al., 2007). Because COUP-TFI and -TFII are highly conserved, similar interactions may also be possible for COUP-TFII. We believe that this may also explain why the established opsin expression pattern persists until adulthood when COUP-TFI is not expressed in mature cone cells.

Finally, we asked whether the unique expression patterns and functions of COUP-TFs are conserved in other animals. In the rat retina, M- and S-opsin are expressed homogeneously throughout the retina, whereas S-opsin is present in only 5%–10% of cones (supplemental Fig. S6A). We found that, similar to the mouse retina, COUP-TFI expression decreased from the ventral to the dorsal side in the rat retina during embryogenesis (supplemental Fig. S6C). In the adult rat retina, we observed that COUP-TFI was expressed in the cells of the GCL and INL and that it maintained its expression gradient (supplemental Fig. S6B). COUP-TFII was detected in the dorsal retinas of rat embryos, which was similar to our findings in the dorsal mouse retina; moreover, we observed weak but clear COUP-TFII expression in other parts of the retina (Supplemental Fig. S6C, two-headed arrow). Based on these findings, we hypothesize that the presence of both COUP-TFI and -TFII throughout the retina in the rat embryos may contribute to the suppression of S-opsin in cone cells, while the presence of COUP-TFII on only the dorsal side of the retina in mouse embryos leads to the suppression of S-opsin in dorsal cone cells. Although opsin expression pattern varies by species, COUP-TFs may commonly regulate the formation of opsin expression patterns. Further elucidation of the molecular mechanism of opsin patterning in mouse retina will shed light on the formation of the human fovea, in which M- and L-opsin are exclusively expressed.

Supplementary Material

Supp1

Acknowledgements

We are grateful to Drs. Y. Yanagi and S. Kato (University of Tokyo) for plasmid pcDNA/FLAG-Crx, Drs. T. Imamura (The JFCR Cancer Institute) and K. Miyazono (University of Tokyo) for plasmids pcDNA3/ALK3QD and pcDNA/ALK6QD (constitutively active type I Bmp receptors), Dr. C.M. Craft (The Keck School of Medicine of the University of Southern California) for anti-mCAR antibody, and Perseus Proteomics Inc. for providing the antibodies. We thank Dr. N. Watanabe in FACS core laboratory for their technical support for the sorting. We thank Dr. Mireille Vasseur-Cognet (INSERM, Paris) for the discussion. This work was supported by a grant-in-aid from the Ministry of Education, Culture, Sports, Science, and Technology of Japan to SW and by an NIH grants to MJT, SYT and YF (EY013128).

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