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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Biochemistry. Author manuscript; available in PMC 2010 June 23.
Published in final edited form as:
PMCID: PMC2789275
NIHMSID: NIHMS162101

Antagonism of human adiponectin receptors and their membrane progesterone receptor paralogs by TNFα and a ceramidase inhibitor.

Abstract

The Progestin and AdipoQ Receptor (PAQR) family of proteins comprises three distinct structural classes, each with seemingly different agonist specificities. For example, Class I receptors, like the human adiponectin receptors (AdipoR1 and AdipoR2), sense proteins with a particular three-dimensional fold, while Class II receptors are non-classical membrane receptors for the steroid hormone progesterone. Using a previously developed heterologous expression system to study PAQR receptor activity, we demonstrate that human PAQRs from all three classes are antagonized by both 1S,2R-D-erythro-2-N-myristoylamino)-1-phenyl-1-propanol, a ceramidase inhibitor, and TNFα, a homolog of adiponectin that functions antagonistically to both adiponectin and progesterone in human cells.

The Progestin and AdipoQ Receptor (PAQR) family of proteins comprises several medically important hormone receptors with links to pathological conditions, including obesity, diabetes and coronary artery disease (1). PAQR proteins can be grouped into three distinct classes based on sequence comparisons. Class I receptors are found in nearly all eukaryotes. The two best-characterized human Class I receptors are the adiponectin receptors, AdipoR1 (PAQR1) and AdipoR2 (PAQR2), which sense adiponectin, an adipose-derived hormone in the C1q/TNF superfamily of animal proteins (1). A third Class I PAQR (Izh2p) from the fungus Saccharomyces cerevisiae senses plant proteins in the PR-5 defensin superfamily whose three-dimensional fold is nearly identical to the β-sandwich of the C1q/TNF superfamily (2, 3). Consequently, it appears as though the unifying feature of Class I PAQRs may be the ability to bind proteins with a specific β-sandwich fold.

On the other hand, the Class II PAQRs, including mPRα (PAQR7), mPRβ (PAQR8) and mPRγ (PAQR5) diverged from Class I receptors after the evolution of metazoans. Intriguingly, the steroid hormone progesterone agonizes these receptors (4, 5) and it is unclear how this functionality evolved from Class I β-sandwich receptors. Finally, there is the enigma of the Class III PAQRs, which have the deepest evolutionary roots but no known agonist. Not only do all metazoans have at least one Class III protein, they are widely, but not universally, dispersed in protozoan and eubacterial proteomes (6). Because Class III receptors predate Class I receptors, one might predict that their most recent common ancestor may have sensed a β-sandwich-like protein.

There is considerable pharmaceutical interest in identifying novel molecules that can agonize human PAQR receptors. In particular, because adiponectin is anti-diabetic, there is considerable interest in finding agonists for AdipoR1 and AdipoR2 that might be useful pharmaceuticals for the treatment of obesity or type II diabetes (1). However, recent studies suggest that these two receptors, while sensing the same agonist, may have opposing physiological roles (7). Consequently, the search for molecules that target PAQRs should be expanded to look for antagonists as well as agonists.

We have developed a yeast-based assay system that can be used to study the functionality of human PAQRs. The details of this assay have been extensively described elsewhere (4, 8, 9) and are summarized in the Supporting Information. In brief, the assay is based on the fact that PAQR receptors in Saccharomyces cerevisiae (named Izh1p, Izh2p, Izh3p and Izh4p) activate an intracellular signaling cascade that negatively controls the expression of a gene called FET3. We generated a β-galactosidase based FET3 promoter-reporter construct whose activity is inversely proportional to the activity of the expressed PAQR receptor.

Using this assay, we demonstrated functional expression of AdipoR1, AdipoR2, mPRγ, mPRα and mPRβ. In addition, we discovered that the human Class I receptor, PAQR3, is activated by adiponectin and renamed it AdipoR3 (9). Moreover, we discovered that the two remaining human Class II receptors, PAQR6 and PAQR9, are agonized by progesterone and renamed them mPRδ and mPRε (4).

We made another intriguing discovery with our yeast-based assay: For the yeast Izh2p and several human receptors (AdipoR1, PAQR3, PAQR4, mPRγ, mPRβ and PAQR11), agonist is not absolutely required to activate the downstream signaling pathway (2, 4, 8, 9). In these cases, maximal overexpression is sufficient to constitutively activate the pathway, indicating that some receptors in this family possess significant basal signaling capability. While we are unsure why some receptors possess such high basal activity, this is an important discovery because it allows us to use our assay to screen for different types of antagonists, including both competitive antagonists and inverse agonists, which are a special type of antagonist that inhibits basal signaling.

We recently published data demonstrating that fungal PAQRs produce a sphingoid base second messenger in yeast by activating an endogenous ceramidase enzymatic activity (2). We also demonstrated that MAPP, a potent ceramidase inhibitor, strongly inhibits both the basal and agonist-inducible signaling capability of the endogenous yeast PAQR, Izh2p. In Figure 1A, Table 1 and the Supporting Figure, we show that MAPP also inhibits the basal signaling of human Class I (AdipoR1, PAQR3, PAQR4), Class II (mPRγ, mPRβ) and Class III (PAQR11) receptors. Moreover, MAPP inhibits the agonist-inducible signaling of AdipoR1, AdipoR2 and mPRγ. The generalized inhibitory effect of MAPP on yeast and human PAQRs is not surprising considering the strong sequence similarity between PAQRs and proteins in the alkaline ceramidase family (2). The fact that MAPP inhibits both the basal and activated signaling of PAQRs indicates that this molecule acts as an inverse agonist, potentially by inhibiting the mechanism of signal transduction. However, it must be stated that we cannot yet rule out the possibility that MAPP acts on the signaling pathway downstream of the receptor rather than directly on the receptor.

Figure 1
(A) MAPP inhibits basal signaling of maximally expressed AdipoR1 and Izh2p (open symbols) as well as AdipoR1 when activated by 100 pM adiponectin at lower expression levels (closed symbols). (B) TNFα inhibits basal and adiponectin-dependent signaling ...
Table 1
Antagonism of human PAQR signaling by MAPP and TNFα.

We also report the remarkable finding that human proteins in the PAQR family, including AdipoR1 and AdipoR2, are unified by antagonism by the inflammatory cytokine, tumor necrosis factor alpha (TNFα), itself a β-sandwich protein in the C1q/TNF superfamily. The C1q/TNF superfamily has two basic subfamilies: C1q-like proteins, which include adiponectin, and TNF-like proteins, which includes TNFα (10). A quick perusal of the literature reveals that TNFα and adiponectin often exert opposite or antagonistic effects in human cells. For example, while there are low levels of adiponectin in obesity, there are high levels of TNFα. More importantly, while adiponectin is anti-diabetic and anti-inflammatory, TNFα is pro-diabetic and pro-inflammatory (11).

Given that TNFα opposes adiponectin and that the two proteins belong to the same structural family, we postulated that TNFα might antagonize the effects of adiponectin on adiponectin receptors. Table 1, Figure 1B and the Supporting Figure show that this is indeed the case for AdipoR1. In addition, TNFα acts as an inverse agonist for AdipoR1, shutting off its basal signaling. (Figure 1B) More importantly, TNFα does not affect signal transduction in yeast when the endogenous Izh2p receptor is used to activate the pathway. These results clearly demonstrate that TNFα does not act on the yeast signaling pathway downstream of the receptors, or else it would have a similar effect on Izh2p. The possibility that TNFα is a more general antagonist of human PAQRs is supported by data shown in Table 1, which demonstrate TNFα-dependent inhibition of basal signaling of PAQR3, PAQR4, mPRγ, mPRβ and PAQR11. Moreover, TNFα antagonizes the effect of agonists on AdipoR1, AdipoR2, PAQR3, mPRγ, mPRδ and mPRβ. The only receptors that do not seem to be affected by TNFα are mPRα and PAQR9.

It is critical to emphasize that the effect of these results show an effect of TNFα in yeast cells expressing human PAQRs, not on human cells. The yeast genome does not encode C1q/TNF family members or homologs of the classical TNFα receptors (TNFR) (12). Hence, the effect of TNFα in our system must be independent of the known mechanisms of sensing TNFα since these systems are absent in yeast. Moreover, since TNFα has no effect on yeast cells carrying empty expression vector or overexpressing an endogenous yeast PAQR, the possibility that a yeast protein mediates the effects of TNFα rather than the expressed human PAQR is very remote. The simplest explanation for our extraordinary finding is that TNFα functions as both a general competitive antagonist as well as an inverse agonist for human PAQRs. Of course, we will need to show direct binding of TNFα to the PAQRs to confirm this model although, it must be noted, direct binding of agonist to receptor has not actually been demonstrated for any PAQR, including AdipoR1. This is mainly due to the fact that no group has yet been able to purify any PAQR receptor for in vitro study.

The physiological importance of TNFα antagonism of human PAQRs is unknown. TNFα exerts maximal effects on heterologously expressed human PAQRs in the low- to mid-nanomolar range, concentrations that are significantly higher than steady state circulating levels of this cytokine in human plasma. Of course, it is possible that the pharmacodynamics of these receptors is fundamentally altered by heterologous expression and that our ID50 values are not relevant to the native system. On the other hand, temporal and spatial spikes in TNFα concentrations can be many orders of magnitude higher than the steady state adjacent to sites of inflammation (13), making our discovery potentially relevant under pathological conditions. Even more intriguing is the possibility that the PAQR receptors are promiscuous with respect to other members of the C1q/TNF family. Since the human genome encodes 32 C1q-like proteins and 19 TNF-like proteins (10, 14), it will be interesting to explore the possibility that these proteins represent physiologically relevant PAQR agonists or antagonists.

Clearly, more experiments must be done to study the exact mechanisms by which TNFα and MAPP inhibit PAQR-dependent signaling in yeast. Moreover, it will be critical to confirm these findings in human cells. However, it is important to convey these findings immediately because they may shed light on many unexplained physiological phenomena pertaining to adiponectin, progesterone and TNFα. For example, while it is well known that TNFα opposes the effects of adiponectin on cells (15), a recent study also showed that TNFα inhibits the effects of progesterone in rat ovary cells (16). Hence, there is already evidence that these findings are more than a mere artifact of the in vitro assay.

It is known that sphingolipids are involved in both adiponectin and progesterone signaling (17, 18). Moreover, ceramide is a known second messenger for TNFα signaling (19), although this relationship is generally attributed to the effect of TNFα on sphingomyelinase expression. For the first time, we can present a reasonable unified mechanism that can explain these disparate observations. Eukaryotic cells possess a regulatory module called the ceramide rheostat (20) that governs critical processes such as proliferation, apoptosis and differentiation. The rheostat keeps a homeostatic balance in the relative ratios of ceramides and sphingoid bases. Since PAQRs stimulate the degradation of ceramides in yeast to produce sphingoid bases (2), they are in a unique position to function as regulators of this rheostat and any inverse agonist or antagonist of the PAQRs, such as MAPP or TNFα, will result in ceramide accumulation. It is intriguing to speculate that PAQR receptors function as a fulcrum in human cells for the ceramide rheostat. When they are agonized, perhaps by C1q-like proteins or progesterone, they tip the balance towards sphingoid base. When they are antagonized, perhaps by TNF-like proteins, they tip the balance towards ceramide.

Supplementary Material

Abbreviations

PAQR
Progestin and AdipoQ Receptor
TNF
Tumor Necrosis Factor
MAPP
1S,2R-D-erythro-2-N-myristoylamino)-1-phenyl-1-propanol
AdipoR
Adiponectin receptor
mPR
membrane progestin receptor
ID50
50% maximum inhibitory dose
ED50
50% maximum effective dose

Footnotes

This research was funded by the National Institutes of Health, (R21DK074812 to TJL) and by the University of Florida Department of Chemistry.

Supporting Information Available: Detailed protocol and supplemental figure. This material is available free of charge via the Internet at http://pubs.acs.org.

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