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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
Biochim Biophys Acta. Author manuscript; available in PMC 2011 January 1.
Published in final edited form as:
Biochim Biophys Acta. 2010 January; 1801(1): 31–41.
Published online 2009 September 15. doi: 10.1016/j.bbalip.2009.09.002

Figure 2

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Inhibition of the carboxylesterase activity of recombinant human CES1 by 27-HC. The data were best fit to an equation that describes partially noncompetitive enzyme inhibition (A) [27]: i = (1−β) * [I]/([I] + Ki). For a partially noncompetitive inhibitor it is assumed that the E S I complex is partially catalytically active (i.e., 0<β<1), and the affinity of substrate for enzyme is unchanged by the inhibitor (i.e., α=1). The combined data from 3–4 independent experiments are shown [error bars represent the intra-assay variation (SD) for each independent experiment, which were performed in triplicate]. Analysis of data using a Dixon plot (shown in B) followed by a replot of the slopes of the Dixon plot versus 1/[S] (shown in C) confirmed a non-competitive mode of inhibition [28].

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