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Cover photograph (Copyright © 2009, American Society for Microbiology. All Rights Reserved.): Localization of muscle LIM protein (MLP) and cofilin 2 (CFL2) at the Z-line of human skeletal muscle sections. Coimmunolabeling of MLP (red) and CFL2 (green) with the specific antibodies reveals that they colocalize in vivo (top row). Immunostaining for CFL2 (green) shows that this protein localizes at the Z-line with α-actinin (red) (second row). MLP (red) is also localized at the Z-line, as shown following costaining with α-actinin (green) (third row). However, costaining for CFL2 (green) and phalloidin (red) indicates that CFL2 is not found across the full length of the thin filaments (fourth row). Images in yellow represent the merged green and red images of that row. (See related article November 2009, vol. 29, no. 22, p. 6046.)

Mol Cell Biol. 2009 December; 29(23): Cover