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Cover photograph (Copyright © 2009, American Society for Microbiology. All Rights Reserved.): Confocal laser scanning microscope image of a single-cell tomont of the parasitic ciliate Ichthyophthirius multifiliis, a common pathogen of freshwater fish. The tomont was stained with 4′,6-diamidino-2-phenylindole (DAPI), which preferentially binds double-stranded DNA, illuminating the large, crescent-shaped macronucleus. Visible throughout the cytoplasm are DAPI-stained endosymbionts, which are newly discovered members of the Alphaproteobacteria and Sphingobacteria. The tomont is approximately 700 μm by 560 μm. (See related article on page 7445.)

Appl Environ Microbiol. 2009 December; 75(23): Cover