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Schizophr Res. Author manuscript; available in PMC 2010 July 1.
Published in final edited form as:
PMCID: PMC2782902
NIHMSID: NIHMS130250

Smaller superior temporal gyrus volume specificity in schizotypal personality disorder

Abstract

Background

Superior temporal gyrus (STG/BA22) volume is reduced in schizophrenia and to a milder degree in schizotypal personality disorder (SPD), representing a less severe disorder in the schizophrenia-spectrum. SPD and Borderline personality disorder (BPD) are severe personality disorders characterized by social and cognitive dysfunction. However, while SPD is characterized by social withdrawal/anhedonia, BPD is marked by hyper-reactivity to interpersonal stimuli and hyper-emotionality. This is the first morphometric study to directly compare SPD and BPD patients in temporal volume.

Methods

We compared three age-gender- and education-matched groups: 27 unmedicated SPD individuals with no BPD traits, 52 unmedicated BPD individuals with no SPD traits, and 45 healthy controls. We examined gray matter volume of frontal and temporal lobe Brodmann areas (BAs), and dorsal/ventral amygdala from 3T magnetic resonance imaging.

Results

In the STG, an auditory association area reported to be dysfunctional in SPD and BPD, the SPD patients had significantly smaller volume than healthy controls and BPD patients. No group differences were found between BPD patients and controls. Smaller BA22 volume was associated with greater symptom severity in SPD patients. Reduced STG volume may be an important endophenotype for schizophrenia-spectrum disorders. SPD is distinct from BPD in terms of STG volume abnormalities which may reflect different underlying pathophysiological mechanisms and could help discriminate between them.

Keywords: Schizotypal personality disorder, Borderline personality disorder, Schizophrenia, MRI, Brodmann area 22, Auditory cortex

1. Introduction

Several previous studies have shown reduced superior temporal gyrus (STG) volume in individuals with schizophrenia (reviewed by Shenton et al., 2001). Recent work has found that schizotypal personality disorder (SPD) patients also show this abnormality but to a lesser extent than in schizophrenia (Buchsbaum et al., 1997; Dickey et al., 1999; Hazlett et al., 2008; Kawasaki et al., 2004; Takahashi et al., 2006), adding further support to existing evidence that SPD is in the schizophrenia spectrum. However, previous studies of STG volume in SPD compared patients with “supernormal” individuals who did not have Axis I or II psychopathology and also had no family history for psychiatric illness, perhaps increasing the likelihood of finding group differences (Kendler, 2003). Many biological findings in personality disorders appear to be present across different personality disorders, representing perhaps vulnerabilities to personality disorders per se (Kendler et al., 2008). Like other findings, it remains unclear whether STG volume abnormalities are specific to SPD or whether they are also present in other personality disorders, such as borderline personality disorder (BPD). This study aims to evaluate the specificity of STG abnormalities in SPD and BPD participants by comparing the volume of this region as well as other temporal and frontal lobe regions.

The diagnosis of SPD, characterized by asocial tendencies, difficulties with language and paranoid, odd behavior and magical thinking, was first introduced in the DSM-III and based on the clinical profiles of patients with “borderline schizophrenia” in the landmark Danish adoption schizophrenia studies (Rosenthal et al., 1971). These studies and earlier formulations of the disorder suggested that relatives of patients with schizophrenia may display deviant psychological functioning but not all signs of schizophrenia, providing evidence for a spectrum of schizophrenia-related disorders. Many of these earlier studies of “borderline schizophrenia” included symptoms related to conceptions of BPD, but diagnostic overlap decreased when borderline criterion for paranoid ideation under stress was introduced in DSM-III-R (Spitzer et al., 1979). BPD, characterized by affective instability and impulsive behavior, was first included as a personality disorder diagnosis in DSM-III (Gunderson and Singer, 1975;Kernberg, 1977). Nonetheless, the co-morbidity of BPD and SPD is not uncommon (e.g., Zanarini et al., 1998).

Morphometric studies examining the temporal lobe in SPD report reduced gray matter volume in STG (Buchsbaum et al., 1997; Dickey et al., 1999; Hazlett et al., 2008; Kawasaki et al., 2004; Takahashi et al., 2006), middle and inferior temporal gyrus (Downhill et al., 2001), and Heschl’s gyrus (Dickey et al., 2002a). Of note, Dickey and colleagues found decreased STG volume in male (2002a) but not female SPD patients (2003). While volume reductions in medial temporal regions, including amygdala and/or hippocampal complex, are reported in schizophrenia (reviewed by Shenton et al., 2001), these findings have not been consistently observed in SPD (Dickey et al., 2002b). Only one study (Suzuki et al., 2005) reported bilateral reductions in both of these structures in SPD, while Dickey et al. (2007) recently reported reduced hippocampal volume in SPD. Volume reductions in frontal cortex regions have also been reported in studies with schizophrenia patients, but several studies suggest these areas may be relatively preserved in SPD (Hazlett et al., 2008; Kawasaki et al., 2004; Suzuki et al., 2005). Our group and others (Haznedar et al., 2004; Takahashi et al., 2004) previously reported no morphologic differences in cingulate gyrus in SPD but, using a larger sample size, we later found smaller cingulate volume compared with healthy controls, particularly in anterior cingulate cortex (ACC) gray matter (Hazlett et al., 2008).

On the other hand, BPD studies examining the frontal lobe have reported reductions in overall frontal lobe volume (Lyoo et al., 1998), left orbitofrontal cortex and right anterior cingulate volume (Tebartz van Elst et al., 2003), and anterior cingulate (BA24) gray matter volume (Hazlett et al., 2005). It should be noted that Tebartz van Elst and colleagues later found no significant differences in these regions using voxel-based morphometric MRI (Rusch et al., 2003). Some studies also report hippocampal (Irle et al., 2005; Zetzsche et al., 2007) and amygdala volume loss in BPD (Driessen et al., 2000; Schmahl et al., 2003;Tebartz van Elst et al., 2003), while others have shown no significant structural differences between BPD patients and controls in these regions (Chanen et al., 2008; New et al., 2007). Another study found smaller hippocampal volume but no differences in amygdala volume (Brambilla et al., 2004). To date, the above-mentioned studies have looked at these patient groups separately and findings suggest SPD patients have smaller STG volume, both SPD and BPD patients have abnormalities in frontal regions, particularly smaller anterior cingulate volume, and amygdala findings are less clear.

The aim of this study was to examine volume of frontal and temporal lobe regions in three age-gender- and education-matched groups: patients with BPD but no SPD traits, patients with SPD but no BPD traits, and healthy controls. This is the first study to directly compare these groups and test whether morphometric abnormalities in these brain regions are specific to one personality disorder and not the other, or whether the two patient groups differ from healthy controls in opposite directions. While both SPD and BPD patients exhibit symptoms that have been linked to temporal and frontal lobe abnormalities, these symptoms differ phenomenologically from one another. Specifically, emotion dysregulation and impulsivity are hallmark features of BPD (Skodol et al., 2002), while blunted affect and executive dysfunction are frequently associated with SPD (Kirrane and Siever, 2000). In this sense, abnormal volume in prefrontal and temporal regions may underlie affect-related symptoms in BPD (Soloff et al., 2008); in SPD, abnormal frontal and temporal lobe volume may be associated with cognitive and/or executive functioning deficits (McCloskey et al., 2005). Due to these different patterns of symptom presentation in our clinical groups, we expected to find different patterns of frontal/temporal structural abnormalities in these groups as well. Of note, since we want to be able to draw conclusions about regional specificity, we looked at a number of different frontal and temporal regions. Specifically, in the frontal lobe, our analyses focused on prefrontal and cingulate cortex Brodmann areas (BAs)— important in attention and the modulation of executive function and emotion. In the temporal lobe, we focused on regions that contribute to executive and emotion processing and have been shown to have dense interconnections: temporal gyrus regions and amygdala (Barbas, 2007; Pandya, 1995; Price, 2007). The specific temporal gyrus regions included primary auditory cortex corresponding to BA41 and BA42 and auditory association areas corresponding to BA22/STG. Also, since fMRI studies (Kim et al., 2003;Whalen et al., 2001) have begun identifying differential roles of amygdaloid subnuclei and their respective connections with the frontal lobe, we parcellated amygdala into dorsal and ventral regions.

Previous work shows that, in SPD, functional impairment such as odd speech is associated with smaller STG volume (Dickey et al., 2003). This finding is consistent with basic research (Binder et al., 2000; Joanisse and Gati, 2003) and schizophrenia work (e.g., Wible et al., 2001) suggesting the importance of temporal lobe function in language processing and production. In order to investigate individual differences in SPD, we examine correlations between symptom severity, including odd speech/thinking symptomatology, and volume of our hypothesized regions of interest.

We hypothesized that our patient groups would show different frontal-temporal volume patterns from each other and the healthy control group. More specifically, we hypothesized that SPD but not BPD patients would demonstrate reduced STG volume compared with controls. We further hypothesized that the patient groups would show reduced ACC volume compared with healthy controls. We expected group differences in STG and ACC to be more robust while amygdala, BA41, BA42 and additional frontal regions would show more subtle differences. Lastly, we investigated individual differences in symptom severity to determine whether greater symptom severity was associated with frontal-temporal abnormalities. Consistent with prior work (Dickey et al., 2003), we hypothesized that greater STG reduction in SPD would be associated with greater degree of abnormal speech and symptom severity.

2. Methods

2.1 Participants

Fifty-two patients meeting DSM-IV criteria for BPD, 27 patients meeting DSM-IV criteria for SPD and 45 healthy controls (Table-1 for demographic details) were included. The groups did not significantly differ in their distribution of age, gender, or highest educational degree earned. All patients were unmedicated at the time of their MRI which we define as off psychoactive medication for a minimum of two weeks (five weeks for fluoxetine) prior to MRI scanning. Exclusion criteria for all participants included severe medical or neurological illness, head injury, or past substance dependence or substance abuse in the prior six months. All participants had a negative urine toxicology screen, and females, a negative pregnancy test on scan day. Written informed consent approved by Mount Sinai and Bronx Veterans Affairs Medical Center Institutional Review Boards was provided by all participants.

Table 1
Demographic and Clinical Characteristics of Borderline Personality Disorder (BPD), Schizotypal Personality Disorder (SPD), and Healthy Controls

2.2 Imaging

MRI acquisition occurred on a 3-Tesla head-dedicated Siemens Allegra scanner. For high resolution structural images and accurate anatomical tracing of our regions of interest, all participants received T1-weighted MP-RAGE (Magnetization Prepared Rapid Gradient Echo) imaging (208 slices with slice thickness=0.82 mm, matrix size=256×256×208, FOV=21 cm, TR=2500 ms, TE=4.38 ms, TI=1100 ms and an 8° flip angle FLASH acquisition). Total imaging time was 20 minutes.

2.3 Brodmann Area and Amygdala Measurements

An analysis of the BAs of the frontal lobe was conducted on coronal MRI slices (Mitelman et al., 2005) using a digitized version of a histologically-based atlas (Perry et al, personal communication, 1991) that includes 33 coronal slice maps of BAs defined by microscopic examination of one post-mortem brain (Figure-1). Three trained researchers, without knowledge of participants’ diagnoses, outlined the amygdala on coronal MRI sections (Figure-2) using methods reported elsewhere (Haznedar et al., 2000). Our inter-tracer intraclass correlation coefficient for amygdala volume=0.82 (area measured on three slices at the 25th, 50th, and 75th percentiles of antero-posterior distance), in the mid-range of amygdala reliabilities ranging from 0.70 to 0.99 in the meta-analysis of Hajek et al. (2008). The amygdala is a difficult structure to trace, and some groups (Moriarty et al., 1997) have reported considerably lower ICC (0.47 on the right and 0.38 on the left). Low inter-rater reliabilities are associated with errors in tracing and tend to create Type II statistical error rather than creating spurious effects with Type I error.

Figure 1
Brodmann Area Regions of Interest
Figure 2
Amygdala Tracing Method

2.4 Statistical Methods

Our histological atlas program obtained data from the 15 BAs within the prefrontal cortex and cingulate gyrus identified by Perry (8–9–10–11–12, 23–24–25, 29, 31–32, 44–45–46–47). Subgroups of these variables developed on a theoretical and anatomical basis were entered into mixed-model multivariate analysis of variance (MANOVA) analyses.

To test for group differences, we conducted parallel 3-group analyses using a MANOVA with Diagnostic Group (healthy controls-vs.-SPD-vs.-BPD) as the between-group factor and all other factors were repeated measures or within-subjects. For the prefrontal cortex analysis, we used a Group × Region (anterior-orbital-dorsolateral) × BA (anterior:BA8-9-10; orbital:BA11-12-47, dorsolateral:BA44-45-46) × Hemisphere (left,right) × Matter type (gray,white) MANOVA. For the cingulate gyrus analysis, we employed a Group × BA within the cingulate arch (BA31-25-24-23-29) × Hemisphere (left, right) × Matter type (gray,white) MANOVA. For the temporal lobe analysis, we conducted a Group × Region (dorsal amygdala, ventral amygdala, BA41, BA42, BA22) × Hemisphere (left, right) MANOVA.

This approach of using multivariate statistics avoids Type I error associated with t-tests for each group contrast, area and hemisphere. We report the multivariate F (Wilks lambda) to adjust probabilities for repeated-measure effects with more than two levels. The dependent variables for these MANOVAs were expressed as relative volume of each of our regions of interest (ROI/whole brain volume). Our previous work shows that relative and absolute volume analyses produce similar findings (Mitelman et al., 2003) and relative volume measures are more conservative. However, we also examined whether there were any group differences in overall whole brain volume with a one-way ANOVA. Whole brain volume was calculated by adding gray and white matter volume for BAs within the four lobes (frontal:BA8-9-10-11-12-32-44-45-46-47; parietal:BA7-39-40; temporal:BA20-21-22-27-28-30-35-36-37-38-41-42; and occipital:BA17-18-19).

Correlational analyses were limited to frontal and temporal cortex regions where between-group differences in volume were significant. For each patient group, we used Pearson’s correlation coefficients to examine the association between STG volume and DSM-IV symptom severity with a significance level of p<0.05, two-tailed.

3. Results

3.1 Temporal Lobe

The MANOVA showed a significant Group × Temporal lobe region interaction, F(8,236)=2.27, p=0.02, Wilks (Figure-3). This interaction reflected a complex pattern indicating SPD patients had significantly smaller STG volume than healthy controls (Fisher LSD, p=0.01) and BPD patients (Fisher LSD, p=0.004) while the BPD group did not differ from controls (Figure-4). In BA42, the BPD group had significantly larger volume compared with healthy controls (Fisher LSD, p=0.03) while the SPD group was intermediate between the BPD and control groups. None of the other post-hoc tests reached significance, indicating that the groups did not differ in dorsal/ventral amgydala or BA41 volume. The main effect of group was not significant, indicating the three groups did not differ in overall temporal lobe volume (averaged over the five temporal lobe regions and hemisphere, p=0.28). None of the other interactions with group were significant, all p-values≥0.63.

Figure 3
Between-group differences in MRI volume
Figure 4
Relative STG by Diagnosis

3.2 Two-Group Temporal Lobe Analyses

We followed-up our significant 3-group MANOVA with a series of 2-group MANOVAs which confirmed that the BPD-vs.-SPD and healthy control-vs.-SPD comparisons also showed significant Group × Temporal Lobe Region interactions (F(4,74)=3.81, p=.007 and F(4,67)=2.47, p=0.05, respectively), whereas the healthy controls-vs.-BPD comparison was not significant.

3.3 Cingulate Gyrus and Frontal Lobe

In our cingulate gyrus 3-group MANOVA, there was no main effect of group (p=0.62) and none of the interactions with group reached significance (all p-values≥0.25). However, in order to examine whether the current healthy control-vs.-SPD without BPD sample resembled our prior sample (Hazlett et al 2008), we conducted a 2-group MANOVA on the cingulate. This analysis showed a trend for a Group × BA interaction (F(4,67)=2.23, p=0.08, Wilks) and the cingulate pattern was similar to our prior finding of smaller BA24 in SPD. Along the same lines, in order to examine whether the current healthy control-vs.-BPD sample resembled our prior BPD sample (Hazlett et al 2005), we conducted a 2-group MANOVA. The Group × BA interaction did not reach significance (p=0.18).

In the prefrontal cortex MANOVA (Anterior (BA8, BA9, BA10), Orbital(BA11,BA12,BA47), Dorsolateral (BA44,BA45,BA46)), there was no main effect of group (p=0.36) and the interactions with group did not reach significance (all p-values≥0.10).

3.4 Whole-Brain Volume Analysis

The three groups did not differ in whole brain volume (Healthy controls: mean=1188±121 cm3); SPD patients: mean=1229±111 cm3; BPD patients: mean=1179±98 cm3) (main effect of group: F(2,121)=1.90, p=0.15).

3.5 Symptom Correlates

We examined symptom correlates in the SPD and BPD patient groups for all temporal cortex regions examined (BA22, BA41, BA42). Among the SPD patients, smaller gray matter volume in STG/BA22 was associated with greater overall clinical severity (r=-0.48, p=0.01) (Figure-5). Additionally, smaller STG volume was associated with greater odd speech (r=-0.49, p=0.01) among SPD patients. We did not find significant associations between BA41, BA42 and other SPD criteria, including cognitive/perceptual, paranoid and interpersonal relations symptoms.

Figure 5
Individual differences in superior temporal gyrus volume and symptom severity

In the BPD group, the relationship between overall BPD clinical severity and BA22 volume was not significant nor was the relationship between overall clinical severity and both BA41 and BA42 volume.

3.6 Addressing Potential Confound of Prior Psychoactive Medication

The majority of patients in our study had never previously received psychoactive medication (Table-1). Those patients who were previously medicated were off all medication for a minimum of two weeks (five weeks for fluoxetine) prior to their MRI. In order to address whether prior medication use could explain our main findings in the temporal cortex, we repeated our 3-group analyses after removing the previously medicated five SPD and 23 BPD patients, and the pattern of results was the same as that observed with the entire sample. This 3-group MANOVA showed no significant main effect of Group, and the Group × Temporal lobe region interaction remained significant, F(8,180)=2.56, p=0.01. Follow-up post-hoc tests confirmed that the SPD group had significantly smaller STG volume than the healthy controls and BPD patients (Fisher’s LSD, p=0.005 and p≤0.001, respectively), while BPD patients did not differ from normal on STG volume. In BA42, the BPD group had significantly larger volume than healthy controls (Fisher LSD, p=0.04) but not SPD patients.

3.7 Addressing Potential Confound of Comorbid Past Major Depressive Disorder

To determine whether our main findings in the temporal cortex could be explained by the issue of past MDD comorbidity (occurring > two months prior to their MRI), we compared subgroups of BPD (n=26) and SPD (n=22) patients with no past MDD and all the healthy controls (n=45). Our temporal lobe findings were the same as that shown in the full sample. Specifically, the 3-group MANOVA showed no significant Group main effect and the Group × Temporal lobe region interaction remained significant, F(8,174)=2.70, p=0.008. Post-hoc tests confirmed that the SPD group had smaller STG volume compared with healthy controls (Fisher’s LSD, p=0.003) and BPD patients (Fisher’s LSD, p=0.001) while BPD patients did not differ from controls. Lastly, the BPD patients showed a trend towards significantly larger BA42 volume compared with healthy controls, (Fisher’s LSD, p=0.07) but exhibited no significant difference in BA42 volume from the SPD group.

4. Discussion

To our knowledge, this is the first morphometric study to compare volume of frontal cortex, temporal cortex and amygdala volume in SPD and BPD patients to determine which regions are important in differentiating these two disorders. This is the first study to report that (a) smaller-than-normal STG volume is specific to SPD and not found in BPD and (b) smaller STG volume in SPD is associated with greater overall symptom severity. Our finding of reduced STG volume in SPD compared with both healthy controls and BPD patients remained significant even when we controlled for history of prior psychoactive medication and comorbid MDD, suggesting it is not due to these factors. Our STG finding is consistent with previous reports of reduced STG volume in SPD compared with healthy individuals (Buchsbaum et al., 1997; Dickey et al., 1999; Hazlett et al., 2008; Kawasaki et al., 2004; Takahashi et al., 2006). Consistent with the work of Dickey et al. (2003), we also found that smaller STG volume in SPD was associated with greater odd speech. Lastly, BA42 volume was significantly larger in BPD patients compared with healthy controls, and between-group differences in the other temporal lobe regions included in our analysis (BA41 and dorsal/ventral amygdala) were not significant.

From a functional perspective, prior work indicates that STG/BA22 has wide connections to the hippocampus-amygdala complex, cortical association areas in the frontal and parietal lobe and the thalamus, and it has been proposed that this part of the temporal cortex is involved in higher-order auditory perception (Binder et al., 1994; Engelien et al., 1995; Rajarethinam et al., 2000; Pandya, 1995). This includes phonemic discrimination and processing, the fusing of multiple sound features and analysis of temporal acoustic features of speech. Additionally, lesioning of this region produces receptive aphasia and deficits in comprehension of verbal language (Demonet et al., 2005). Considered together with these anatomical findings, the posterior STG localization of our results in both patient groups fits well with work showing that both SPD and BPD individuals exhibit auditory information processing deficits that are similar to one another, as measured by P300 auditory evoked potentials (Kutcher et al., 1989).

As such, our finding demonstrates that volumetric abnormalities in BA22 are unique to SPD and may be an important tool for differential diagnosis between these two disorders. Although it is still unclear how abnormalities in this region can affect auditory processing, it would be worthwhile to investigate the relationship between alterations in BA22 volume and socially-related functions since auditory processing plays a crucial role in social function and may be one way to distinguish between the groups clinically. For example, BA22 is located on the caudal two-thirds of the STG, an area that Gallagher and Frith (2003) found to be activated during “mentalizing” tasks involving interpretation of mental states of others. Thus, our finding may help differentiate between “mentalizing” deficits experienced by both patient groups.

The finding of reduced gray matter volume in BA22 is the most widely-replicated finding in schizophrenia (reviewed by Shenton et al., 2001). Therefore, our finding of reduced STG volume in SPD provides additional evidence for the similarity between SPD and schizophrenia patients. Our prior work showed a schizophrenia spectrum pattern for reduced STG volume with schizophrenia patients exhibiting the largest reduction compared with healthy controls, and SPD patients intermediate (Hazlett et al., 2008). This observation is consistent with prior work indicating that individuals with SPD share a broad range of similarities with schizophrenia patients in terms of genetics and neurobiology (Siever and Davis, 2004).

Our other interesting finding is that, among the SPD patients, smaller relative BA22 gray matter volume was associated with greater overall clinical (DSM) symptom severity, including greater impairment due to odd thinking and speech. These results are consistent with clinical correlates previously reported by Dickey et al. (2003) and provide further evidence that STG abnormalities may underlie the pathophysiology of SPD. A better understanding of the relationship between structural and functional abnormalities in this key language processing area and psychopathology should be a top priority for future work.

We also found larger-than-normal BA42 gray matter volume in BPD patients. This area makes up part of the primary auditory cortex and lies in Heschl’s gyrus, a region shown to be involved in sensory auditory processing. As of April 2009, there were no publications reporting morphology of BA42 in BPD patients but it is possible that increased volume or alterations in this region reflect some type of compensatory mechanism for other dysfunctional regions involved in auditory-processing demands. In previous work, BA42 volume was found to be smaller in males with SPD compared with controls (Dickey et al., 2002a). Even though BA42 makes up only a portion of Heschl’s gyrus and other work has shown preserved volume of this region in SPD (Takahashi et al., 2006), it is important to note possible reasons for discrepancies between our failure to find healthy control-SPD differences in BA42 volume and findings of reduced left Heschl’s gyrus gray matter volume by Dickey et al. (2002a). Differences may be due to heterogeneity of sample characteristics and symptom severity, methodological techniques, normal variations in the distribution of auditory cortex (Dickey et al., 2008) or medication differences. Understanding both structural and functional abnormalities in this region in BPD and SPD patients should remain a goal for future work such that measures of temporal lobe integrity may ultimately help us predict which patients will respond to different treatments.

For the amygdala, a temporal lobe region shown to have dense interconnections with the STG, no significant group differences were detected. Although some studies have shown smaller amygdala volume in BPD compared with healthy controls (Driessen et al., 2000; Rusch et al., 2003; Schmahl et al., 2003; Tebartz et al., 2003), we (New et al., 2007) and others (Brambilla et al., 2004) have found no groups differences. Perhaps amygdala abnormalities in BPD may be more functional than structural. The issue of amygdala volume requires further investigation.

Our frontal lobe analyses were not statistically significant, indicating the three groups did not differ in anterior-orbital-dorsolateral or cingulate gyrus gray matter volume. These prefrontal cortex results are consistent with our previous findings and other observations showing prefrontal volumes largely preserved in SPD in contrast to widespread prefrontal reductions in schizophrenia (Buchsbaum et al., 2002; Suzuki et al., 2005). Contrary to our hypothesis, neither patient group showed significantly smaller ACC volume compared with healthy controls. We previously reported significantly reduced cingulate (BA24, 31, 23) gray matter volume in a sample of 79 patients meeting DSM criteria for SPD compared with healthy controls (Hazlett et al., 2008). However, it should be noted that in our prior study, the SPD group may have had comorbid BPD; this was not examined because the SPD group was compared with schizophrenia patients. We have also previously shown that, compared with healthy controls, individuals with a diagnosis of both SPD and BPD exhibit greater BA24 reduction than those with only BPD (Hazlett et al., 2005). In contrast, our current sample included SPD patients without BPD. Thus, patient sample characteristics such as SPD-BPD comorbidity appear to play an important role in anterior cingulate volume abnormalities and may explain discrepancies across studies.

In the prefrontal cortex, the BPD patients showed no significant gray matter volume difference which is consistent with previous work by our group and others (Hazlett et al., 2005; Rusch et al., 2003). Nonetheless, as mentioned above, the current study did not replicate our previous findings of normal-BPD differences in anterior cingulate gray volume (Hazlett et al., 2005) which has also been reported by Tebartz van Elst et al (2003). This inconsistency may be due to sample differences in clinical symptom characteristics and severity (e.g., 50% of current sample had past history of MDD versus 76% of Hazlett et al 2005 sample).

To the best of our knowledge, this is the first volumetric study to compare SPD patients with BPD patients and healthy controls. Although all patients were unmedicated at the time of their MRI, a unique feature of the study since many MRI studies to date examine medicated patients, a primary limitation is that some had previously received psychoactive medications. However, it is important to note that when we controlled for prior medication history, our finding of smaller STG volume in SPD compared with healthy controls and BPD patients remained significant. A second limitation is that, due to power considerations, we did not include participants with a comorbid diagnosis of both BPD and SPD. This is an important group to include in future studies. Additionally, given the common comorbidity between BPD and MDD, half of the BPD patients in our study met criteria for past MDD, although it was required to be in full remission for at least two months prior to their scan. It is imperative that future BPD studies recruit large enough sample sizes so that individuals with and without past MDD can be compared. As such, it is important to note that our finding of smaller-than-normal STG volume in SPD remained significant when we controlled for past MDD (only 18% of the SPD patients had past MDD). Also, although our diagnostic groups were matched on age and gender, it is possible that gender differences exist between groups in morphometry. Unfortunately, given our current sample size, we were unable to examine whether gender effects play a role in our STG findings. Future MRI studies with larger sample sizes will allow a much-needed examination of gender effects in SPD and BPD. Lastly, we do not have family history records for our patients. It would be worthwhile to know how many SPD and BPD patients have a first-degree relative in the schizophrenia spectrum and/or bipolar spectrum and whether there is overlap in family history. Nevertheless, our STG findings suggest that future work addressing the genetic link between reduced STG volume and schizophrenia-spectrum disorders may be fruitful.

Our results showed that SPD individuals exhibit abnormalities in STG volume that are not evidenced in BPD patients, supporting the hypothesis that they are two separate disorders. Of note, neither previous use of psychoactive medications nor past history of MDD explained smaller STG in SPD patients. Our findings are consistent with the concept that SPD is in the schizophrenia spectrum. STG volumetric abnormalities may serve as important biological markers or endophenotypes which may ultimately help diagnose and/or predict treatment response in schizophrenia-spectrum illnesses.

Acknowledgement

This work was supported in part by NIMH grants MH073911 to Dr. Hazlett, MH067918 to Dr. New, and VA MERIT grant 7609-28 to Dr. Siever. Other support came from Grant Number M01- Patient Care RR00071 from the National Center for Research Resources (NCRR).

Role of the funding source

The formulation of this manuscript has had full support of the study sponsors recognized in the acknowledgments. No interference has been made by them in the study design; collection, analysis, and interpretation of data; and our sponsors served no role in the writing the report; or in the decision to submit the paper for publication.

Footnotes

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There are no conflicts of interest for any of the authors.

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