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Philos Trans R Soc Lond B Biol Sci. 2009 November 27; 364(1534): 3341–3350.
PMCID: PMC2781849

Effects of heat stress on mammalian reproduction

Abstract

Heat stress can have large effects on most aspects of reproductive function in mammals. These include disruptions in spermatogenesis and oocyte development, oocyte maturation, early embryonic development, foetal and placental growth and lactation. These deleterious effects of heat stress are the result of either the hyperthermia associated with heat stress or the physiological adjustments made by the heat-stressed animal to regulate body temperature. Many effects of elevated temperature on gametes and the early embryo involve increased production of reactive oxygen species. Genetic adaptation to heat stress is possible both with respect to regulation of body temperature and cellular resistance to elevated temperature.

Keywords: heat stress, reproduction, spermatogenesis, oocyte, embryo, gestation

1. Introduction: maintenance of homeothermy and consequences of regulation and dysregulation of body temperature for reproduction

As endotherms, mammals typically function at high core body temperatures that range from approximately 35°C to 39°C (Prosser & Heath 1991). These high temperatures, which generally exceed the temperature of the surrounding environment, are achieved through combustion of fuel stuffs to achieve a high metabolic rate (i.e. heat production). Body temperature is closely regulated by matching heat production with heat loss to the environment via conduction, convection, radiation and evaporation. The set-point temperature for regulation of body temperature is not fixed but can vary diurnally or, in some animals, as part of the hibernation response or in response to changes in environmental temperature or other cues (Heldmaier et al. 2004).

As a group, endotherms can better tolerate low body temperatures than high body temperatures. Indeed, many hibernating species maintain core body temperature at 6–10°C or less (Heldmaier et al. 2004). There is less resistance to body temperatures above the set-point temperature: death is likely when temperatures exceed these values by a few degrees because of disruptions in membrane fluidity, protein structure and, for animals in which sweating or panting exist, electrolyte and fluid loss. In humans, for example, set-point temperature is 37°C and potentially lethal effects of hyperthermia are common at body temperatures above 40–41°C (Jardine 2007). Not surprisingly then, regulation of core body temperature is a priority over several other physiological functions. Regulation of body temperature in endotherms can be considered as a homeokinetic control process whereby achievement of equilibrium in body temperature involves dynamic processes that lead to perturbations in other physiological processes.

Heat stress in this paper is defined as an environment that acts to drive body temperature above set-point temperature. Heat stress can lead to disruptions in reproductive processes through two general mechanisms. First, the homeokinetic changes to regulate body temperature can compromise reproductive function. One example is redistribution of blood flow from the body core to the periphery to increase sensible heat loss. Another homeokinetic control mechanism for body temperature is reduced feed intake during heat stress. Reducing feed intake reduces metabolic heat production but also can lead to changes in energy balance and nutrient availability that can have large effects on cyclicity, establishment of pregnancy and foetal development. A second mechanism for disruption of reproduction during heat stress is the failure of homeokinetic systems to regulate reproduction. As will be seen in this paper, the rise in body temperature about its regulated set point can compromise function of germ cells, the early developing embryo, and perhaps other cells involved in reproduction. In this paper, the term heat shock will be used to describe the effects of elevated temperature on cellular function.

One objective of this paper is to describe the consequences of heat stress for specific components of the reproductive process in the male and female mammal. Most of the work on this topic has involved farm animals because of the economic consequences of heat stress in livestock systems and the paper will necessarily focus largely on these species. A second objective is to highlight the genetic plasticity of mammals with respect to adjusting to changes in the thermal environment. In large part, endotherms occupy a large percentage of the Earth's surface because of evolutionary advantages in maintaining a constant high body temperature in the presence of a variable environmental temperature and the effectiveness of thermoregulatory mechanisms in maintaining that body temperature. Thus, there is capacity for adaptation during both physiological and evolutionary time-scales.

2. The male

(a) Spermatogenesis

Most mammals have testes suspended in a scrotum outside the body cavity so that intratesticular temperature is slightly lower than core body temperature. There is an intricate thermoregulatory system in the testis involving countercurrent exchange of heat from warm blood entering the testis and cool blood draining from the testis through an arterio-venous plexus called the pampiniform plexus. The degree of cooling is further controlled by two muscles: the tunica dartos in the scrotum that regulates scrotal surface area and the cremaster muscle that controls the position of the scrotum relative to the body.

It has been speculated that evolution of the scrotum occurred because of the need for low temperatures either for spermatogenesis, sperm storage or to minimize mutations in gamete DNA (Werdelin & Nilsonne 1999; Bedford 2004). However, low body temperature is not an absolute requirement for spermatogenesis. Birds, which have body temperatures higher than mammals (Prosser & Heath 1991), have internal testes. So do several mammalian species (figure 1). Seals, for example, have testes that have descended to the ventral body wall and which may experience some local cooling via anastomoses of veins leaving the dorsal flipper with veins supplying venous plexuses in the inguinal region (Rommel et al. 1995). Other mammals such as cetaceans (Rommel et al. 1992) and elephants (Gaeth et al. 1999) have testes that do not descend towards the inguinal region and which have temperatures that are the same as core temperature (elephant; Werdelin & Nilsonne 1999) or are presumably the same (cetacean).

Figure 1.

Variation in the location of the testes in mammals. Most mammals have testes that are located outside the abdominal cavity and that exist at a temperature a few degrees cooler than core body temperature. One example is the (c) horse. However, in some ...

Regardless of the evolutionary reason for the location of the testis and epididymis outside the body, a rise in testicular temperature in mammals with external testes leads to reduced sperm output, decreased sperm motility and an increased proportion of morphologically abnormal spermatozoa in the ejaculate. Such effects can be observed when a local heat source is applied to the testis, the scrotum is insulated, the testes are internalized (i.e. cryptorchidism induced) or body temperature is raised because of fever or thermal environment (Setchell 1998). The cells that are most susceptible to damage are the spermatocyte and spermatid (Setchell 1998), although B spermatogonia can be damaged also (figure 2). Oxidative stress is a major cause for thermal damage of spermatogenic cells and leads to apoptosis and DNA strand breaks (Pérez-Crespo et al. 2008; Paul et al. 2008, 2009). Effects of cryptorchidism on spermatogenesis were enhanced in superoxide dismustase-1 knockout mice (Ishii et al. 2005).

Figure 2.

DNA damage in epididymal sperm collected at various times after heating of the scrotum of male mice. DNA damage was determined using the TUNEL assay. The x-axis indicates the days after heating when the sample was collected and the approximate stage at ...

There are indications that developmental competence of the resulting embryo can be reduced if fertilization is by a spermatozoon exposed to heat shock. Paul et al. (2008) reported that in vitro fertilization with sperm recovered from male mice in which the scrotum was heated to 42°C resulted in embryos with reduced ability to complete development. In addition, females mated to males exposed to scrotal heating had conceptuses with smaller foetal and placental weights compared with controls (Jannes et al. 1998; Paul et al. 2008).

Semen characteristics are not immediately affected by changes in testicular temperature because damaged spermatogenic cells do not enter ejaculates for some time after heat stress. In the bull, for example, where spermatogenesis takes about 61 days, alterations in semen occur about two weeks after heat stress and do not return to normal until up to eight weeks following the end of heat stress (figure 3).

Figure 3.

Time course of effects of heat stress on motility of ejaculated spermatozoa in bulls. Control bulls (circles) were maintained in a chamber at 23 ± 1°C for 16 weeks. Heat-stressed bulls (triangles) were maintained for 8 weeks in a chamber ...

Data are equivocal as to whether ejaculated spermatozoa can be damaged by heat shock when deposited in the reproductive tract of a hyperthermic female. Culture of bull spermatozoa at 40°C did not alter fertilizing capability or the competence of the resultant embryos to develop to the blastocyst stage (Hendricks et al. 2009). In addition, ejaculated bull and stallion spermatozoa do not undergo apoptosis when cultured at temperatures characteristic of physiological hyperthermia (Hendricks & Hansen 2009). Nonetheless, there may be epigenetic changes in embryonic development associated with damage to the sperm in the reproductive tract. Insemination of rabbit does with sperm exposed to elevated temperature in vitro (Burfening & Ulberg 1968) or in the female reproductive tract (Howarth et al. 1965) resulted in reduced preimplantation survival and, in one study (Burfening & Ulberg 1968), post-implantation survival. There is also evidence that X and Y spermatozoa are affected differentially by elevated temperature. The sex ratio of embryos was skewed towards female when female mice were bred to males experiencing scrotal heat treatment on the day of mating (Pérez-Crespo et al. 2008). In contrast, incubation of sperm at 40°C for 4 h when compared with 38.5°C tended to reduce the proportion of embryos that were female following in vitro fertilization (Hendricks et al. 2009).

(b) Hormone secretion

There are few experiments on the effects of elevated environmental temperature on the secretion of hormones controlling reproductive function. Data from both bulls and boars indicate that heat stress causes an initial decline in circulating concentrations of testosterone lasting two weeks but that concentrations become restored even in the face of continued heat stress (Rhynes & Ewing 1973; Wettemann & Desjardins 1979). Hyperthermia can alter luteinizing hormone (LH) secretion in females, even in the absence of ovarian steroids (Schillo et al. 1978), and it is likely that severe heat stress can compromise LH secretion in males as well. However, the major site for disruption of reproductive function appears to be the spermatogenic cell lineage in the testis.

3. The female

(a) The oocyte

As for male gametes, heat stress can disrupt development and function of the oocyte. The best evidence for this statement comes from the lactating dairy cow. In this animal, which is particularly sensitive to heat stress because of the metabolic demands of lactation, oocyte competence for fertilization and subsequent development is reduced during times of the year associated with heat stress (Zeron et al. 2001; Al-Katanani et al. 2002; Sartori et al. 2002). There is abundant evidence that heat stress can compromise the oocyte and the follicle in which it is encased. High air temperatures 10 days before oestrus were associated with low fertility (Al-Katanani et al. 1999). Steroid production by cultured granulosa and thecal cells was low when cells were obtained from cows exposed to heat stress 20–26 days previously (Roth et al. 2001a), i.e. when follicles were 0.5–1 mm in diameter. Moreover, the resumption of fertility seen in lactating dairy cows in Israel in the autumn could be hastened by removing follicles formed in the summer (Roth et al. 2001b).

The mechanism by which heat stress during oogenesis compromises oocyte function is likely to involve alterations in follicular function. Heat stress can alter follicular growth (Roth et al. 2000), steroid secretion (Wolfenson et al. 1997; Roth et al. 2001a; Ozawa et al. 2005) and gene expression (Argov et al. 2005). In goats, heat stress reduced plasma concentrations of oestradiol and lowered follicular oestradiol concentration, aromatase activity and LH receptor level, and delayed ovulation (figure 4; Ozawa et al. 2005). In rats, heat stress reduced the levels of gonadotropin receptors and aromatase activity of granulosa cells and the follicular fluid concentrations of oestradiol (Shimizu et al. 2005).

Figure 4.

Effects of heat stress on characteristics of follicles during the peri-ovulatory period in goats. Goats were heat stressed (black bar) or not (open bar) for 48 h, treated with prostaglandin F-2α and after 48 additional hours, follicles that had ...

Effects of heat stress on follicular function could involve changes at the level of the follicle or the secretion of the pituitary hormones that control development of the follicle. Cultured follicular cells experience reduced steroid production at elevated temperature, at least in cattle (Wolfenson et al. 1997; Bridges et al. 2005). Also, follicular responsiveness to LH, as measured by oestradiol release after injection of gonadotropin releasing hormone injection, was reduced by heat stress in goats (Kanai et al. 1995). Heat stress can reduce LH secretion (Schillo et al. 1978; Wise et al. 1988). One of the consequences of heat stress in lactating dairy cows is increased numbers of small and medium follicles; recruitment of these follicles into the growing pool seems to be due to a decrease in circulating concentrations of inhibin and increased FSH secretion (Roth et al. 2000).

The oocyte remains susceptible to heat stress through the preovulatory period. As shown in cows (Putney et al. 1989) and mice (Baumgartner & Chrisman 1988; Aroyo et al. 2007; Roth et al. 2008), experimental heat stress coincident with ovulation and oocyte maturation may or may not have an effect on the capacity of oocytes to be fertilized but the resultant embryos are more likely to develop slowly or abnormally. Damage to the oocyte during the preovulatory period could reflect hormonal perturbations. In addition, the process of oocyte maturation is disrupted at elevated temperature (Ju & Tseng 2004; Payton et al. 2004; Roth & Hansen 2005; Wang et al. 2009). Damage to the oocyte during the preovulatory period by heat shock seems to involve the generation of reactive oxygen species, as both the effects of heat stress in vivo (Roth et al. 2008) and heat shock in vitro (Lawrence et al. 2004) were reduced by administration of antioxidants. Apoptosis plays a critical role in effects of thermal stress on the maturing oocyte in cattle. A fraction (approx. 15–30%) of oocytes exposed to elevated temperature undergoes apoptosis as determined by TUNEL labelling of the pronucleus (Roth & Hansen 2004a,b, 2005; Soto & Smith 2009). Inhibition of heat-shock-induced apoptosis with a caspase inhibitor (Roth & Hansen 2004a), sphingosine 1-phosphate (Roth & Hansen 2004b, 2005) or a BH4 peptide (Soto & Smith 2009) reduced the effect of elevated culture temperature on oocyte competence for fertilization and subsequent development (figure 5).

Figure 5.

Inhibition of apoptosis with the caspase inhibitor z-DEVD-fmk reduces the effects of heat shock on oocyte maturation. Bovine oocytes were matured at either 38.5°C or 41°C in the presence or absence of z-DEVD-fmk. Subsequently, oocytes ...

(b) Embryonic development

The preimplantation embryo is susceptible to maternal heat stress but the susceptibility declines as development proceeds. In cattle, for example, Ealy et al. (1993) found that exposure of lactating cows to heat stress at day 1 after oestrus, when embryos were one to two cells, reduced the proportion of embryos that developed to the blastocyst stage at day 8 after oestrus. However, heat stress at days 3 (8–16 cells), 5 (morula) and 7 (blastocysts) had no effect on the proportion of embryos that were blastocysts at day 8. A similar pattern of developmental acquisition of thermal resistance occurs in sheep (Dutt 1964) and pigs (Tompkins et al. 1967). The adverse effects of heat shock on cultured embryos also is reduced as they become more advanced in development, at least in the cow (Edwards & Hansen 1997; Krininger et al. 2002; Sakatani et al. 2004) (figure 6). In contrast, there is no large difference in sensitivity to elevated culture temperatures between mouse embryos at the two-cell, four-cell and morula stages of development (Aréchiga & Hansen 1998).

Figure 6.

Bovine preimplantation embryos become more resistant to heat shock as development advances. Shown are the results of three experiments (Experiment 1, Edwards & Hansen 1997; Experiment 2, Krininger et al. 2002; Experiment 3, Sakatani et al. 2004 ...

Some actions of elevated temperature on the preimplantation embryo probably involve increased production of reactive oxygen species. The best evidence for this idea comes from the mouse. Maternal heat stress resulted in increased reactive oxygen species activity in oviducts and embryos (Ozawa et al. 2002; Matsuzuka et al. 2005a,b) and reduced glutatione content in recovered embryos (Ozawa et al. 2002; Matsuzuka et al. 2005b). Moreover, treatment of female mice with either melatonin (Matsuzuka et al. 2005b) or vitamin E (Sakamoto et al. 2008) reduced the effects of heat stress on embryonic development. Female embryos are better able to survive effects of elevated temperature than male mice and this gender difference has been demonstrated to be caused by reduced reactive oxygen species production in females (Pérez-Crespo et al. 2005). Increased reactive oxygen species production in response to elevated culture temperature has also been reported for cattle (Sakatani et al. 2004, 2008) and treatment with the antioxidant 2-mercaptoethanol has been reported to alleviate the negative effects of heat shock on development in one study (Sakatani et al. 2008), although not in another (de Castro e Paula & Hansen 2008).

There are several reasons why embryos gain resistance to elevated temperature as development proceeds. Generation of reactive oxygen species in response to heat shock declines as bovine embryos advance in development (Sakatani et al. 2004) while intracellular concentrations of the cytoplasmic antioxidant glutathione increase (Lim et al. 1996). In addition, there is developmental regulation in the capacity of the embryo to undergo the induced thermotolerance response, whereby exposure to a mild elevation in temperature makes cells more resistant to a subsequent severe elevation in temperature. This phenomenon does not develop until day 4 in cattle (Paula-Lopes & Hansen 2002a) and the eight-cell stage in mice (Aréchiga et al. 1995). Acquisition of the capacity for induced thermotolerance involves synthesis of heat shock protein 70 (HSP70). This protein, which stabilizes intracellular proteins and organelles and inhibits apoptosis (Brodsky & Chiosis 2006), can be induced by elevated temperature as early as the two-cell stage in cattle (Edwards & Hansen 1996) and mice (Christians et al. 1997), i.e. before induced thermotolerance is acquired. Therefore, other molecular systems must be involved. Glutathione is required for induced thermotolerance in mice (Aréchiga et al. 1995) and changes in redox status may be an important determinant of development of induced thermotolerance.

Inhibition of apoptosis in bovine embryos with a caspase inhibitor increased the magnitude of the reduction in development caused by elevated temperature (Paula-Lopes & Hansen 2002b). Thus, apoptosis, if limited to the most damaged cells of the embryo, may allow the embryo to continue to develop after an environmental insult. In cattle, induction of apoptosis by elevated temperature does not occur until the 8–16 cell stage at day 4 after insemination (Paula-Lopes & Hansen 2002a).

Some effects of elevated temperature on embryonic survival in utero could be the result of changes in maternal physiology rather than a direct effect on the embryo. In particular, there are reports that heat stress can reduce circulating concentrations of progesterone (see Wolfenson et al. 2000 for review).

(c) Foetal development

Heat stress during gestation causes reduced foetal growth. The mechanisms involved in this phenomenon have been best characterized in sheep. Exposure of pregnant ewes to heat stress causes reduced foetal and placental weights and concentrations of placental hormones in the blood; effects on growth are greater when occurring during mid-gestation than when occurring during later gestation (see Wallace et al. 2005 for review). Some effects of heat stress on placental function represent redistribution of blood to the periphery and reduced perfusion of the placental vascular bed (Alexander et al. 1987). However, reduced perfusion to the placenta is not the only cause of reduced foetal weights because placental blood flow per gram of foetus was similar between heat-stressed and control ewes in the study of Wallace et al. (2005). Perhaps more important is an increase in vascular resistance in the placenta (Galan et al. 2005) caused by alterations in angiogenesis as reflected by aberrant patterns of expression of genes such as vascular endothelial growth factor and its receptors and placental growth factor (Regnault et al. 2002). Heat stress probably has more effects during mid-gestation than late gestation because angiogenesis is more extensive in the former period. Glucose transport capacity across the placenta is also reduced by maternal heat stress (Thureen et al. 1992) and this effect involves reduced expression of GLUT8 genes in cotyledonary placenta (Limesand et al. 2004).

Similar effects of maternal heat stress on placental function and foetal development occur in the cow (Collier et al. 1982). In this species, and presumably others, reduced secretion of placental hormones as a result of heat stress can cause reduced milk yield (Collier et al. 1982; Wolfenson et al. 1988). Therefore, inadequate nutrition for the neonate could conceivably be one consequence of maternal heat stress during gestation.

Maternal hyperthermia can also increase the incidence of teratologies (Graham et al. 1998). Even in the absence of such gross developmental defects, it is possible that foetal stress caused by hyperthermia results in changes in physiological function in adulthood. The idea that events in foetal life affect physiology during adult life (Barker 2007) is now well accepted, although there is little information about the specific case of maternal heat stress. One example is for guinea pigs, where heat stress in utero reduced learning activity in adulthood (Jonson et al. 1976).

4. Genetic plasticity controlling the magnitude of heat stress effects

The gene pools of mammals contain allelic variants of specific genes that control body temperature regulation and cellular responsiveness to hyperthermia. Thus, genetic selection, both natural and artificial, can modulate the impact of heat stress on reproductive function.

The importance of genetics for controlling reproductive response to heat stress is illustrated in figure 7 which shows genetic differences among lines of boars in seasonal variation in sperm output. While all three genetic lines of boars experienced a decrease in sperm concentration in the ejaculate during the summer, the magnitude of the decrease was less for line A than for the other lines and the duration of the decreased sperm output during summer was longer for line B than for lines A or C (Flowers 2008).

Figure 7.

Differences between genetic lines of boars in seasonal variation in sperm output per ejaculate. Square, genetic line A; triangle, genetic line B; circle, genetic line C. The figure is adapted from Flowers (2008) with permission from Theriogenology ...

Genetic influences on regulation of body temperature have been well studied in cattle. In this species, estimates of the heritability of rectal temperature range from 0.25 to 0.65°C (Finch 1986). There are distinct breed differences in thermoregulatory ability (Hammond et al. 1996; Hansen 2004; Pereira et al. 2008). One specific gene affecting body temperature regulation during heat stress, the slick gene affecting hair length, has been identified (Olson et al. 2003; Dikmen et al. 2008) and there are undoubtedly others. The superior thermoregulatory ability of zebu cattle has been ascribed to lower metabolic rate, reduced resistance to heat flow from the body core to the periphery and properties of the hair coat (Hansen 2004).

Genetic variability also exists for tissue resistance to elevated temperature. The adverse effect of heat shock on development of preimplantation bovine embryos was less for breeds of cattle that evolved in hot climates (Brahman, Romosinuano, Nelore) than for breeds that evolved in cooler climates (Angus, Holstein) (Paula-Lopes et al. 2003; Hernández-Cerón et al. 2004; Barros et al. 2006). In addition, the fertility of Holstein cows inseminated during heat stress was greater if semen was from bulls of the Gyr breed, a Bos indicus, than if semen was from Holstein bulls (Pegorer et al. 2007).

In mice, there are strain differences in the testicular response to cryptorchidism (Kon & Endoh 2001; Kazusa et al. 2004) and scrotal heating (Li et al. 2009). For example, surgical cryptorchidism of males from A/J BALB/c, C3H/He, and C57BL/6 strains resulted in loss of most germ cells and a reduction in testicular size of between 56 and 62 per cent compared with the control testis maintained in the scrotum. For mice of the MRL/Mpj strain, large numbers of germ cells remained in the cryptorchid testis and the reduction in size compared to the control testis was only 31 per cent (figure 8) (Kon & Endoh 2001). Similarly, scrotal heating of 43°C induced germ cell apoptosis and loss in both C57BL/6 and AKR/N males but the magnitude of the effects was greater for C57BL/6 (Li et al. 2009). The resistance of germ cells of MRL/Mpj mice to elevated testicular temperature may be caused by a mutation in exonuclease-1 (Namiki et al. 2003).

Figure 8.

Strain differences in mice for testicular changes following surgical cryptorchidism. Shown are cross sections of testicular tissue from (a) C57/BL6 and (b) MRL/Mpj mice. Note that the seminiferous tubules were larger in MRL/mpj mice and that germ cells ...

5. Synopsis: consequences of actions of climate change on reproduction for species survival and distribution

Clearly, heat stress can have large effects on most aspects of reproductive function—male and female gamete formation and function, embryonic development and foetal growth and development. The potential impact of heat stress on a mammalian population can be seen by examining seasonal trends in reproductive function of livestock species. In a study in Spain, for example, where heat stress is frequent in the summer, the proportion of inseminated dairy cows that become pregnant during the warm months of the year was 22.1 versus 43.1 per cent of cows inseminated in the cool season (López-Gatius 2003). One must be cautious in extrapolating data from domesticated animals used for food production to wild populations of animals because selection for growth or milk yield increases metabolic rate and exacerbates the problem of body temperature regulation during heat stress. Indeed, the magnitude of the summer decline in fertility is much less for non-lactating heifers or cows producing low amounts of milk than it is for cows with high milk yield (Badinga et al. 1985; Al-Katanani et al. 1999). Thus, it is likely that the direct impact of global warming (i.e. consequences for body temperature regulation) on mammalian reproduction will be more severe for domestic animals than for wild mammalian species. In addition, the existence of allelic variation in genes controlling body temperature regulation and cellular resistance to heat shock means that genetic adaptation to increasing global temperature will be possible for many species.

Acknowledgements

The author's research on this topic was supported by National Research Initiative Competitive Grants Program grant no. 2007-35203-18070 from the US Department of Agriculture Cooperative State Research, Education and Extension Service and by Research grant no. US-3986-07 from BARD, the Binational Agricultural Research and Development Fund.

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