PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of jbacterPermissionsJournals.ASM.orgJournalJB ArticleJournal InfoAuthorsReviewers
 
J Bacteriol. 1963 January; 85(1): 230–236.
PMCID: PMC278112

STUDIES ON IMMUNITY IN ANTHRAX X.

Gel-Adsorbed Protective Antigen for Immunization of Man

Abstract

Puziss, Milton (Fort Detrick, Frederick, Md.) and George G. Wright. Studies on immunity in anthrax. X. Gel-adsorbed protective antigen for immunization of man. J. Bacteriol. 85:230–236. 1963.—Protective antigen in filtrates of anaerobic cultures of Bacillus anthracis was not readily precipitable by alum or other aluminum salts, but was adsorbed onto a preformed aluminum hydroxide gel under specific conditions. The adsorbed product was highly effective in immunizing rabbits, guinea pigs, and mice against challenge with virulent B. anthracis spores. Gel-adsorbed anaerobic antigens preserved with 1:10,000 thimerosal were unstable on storage at 4 C; replacement of this preservative with 1:40,000 benzethonium chloride produced a more stable product. Addition of 0.0009% formalin further increased the stability during accelerated aging at 37 C. The final product had high protective activity in animals and was well tolerated in man.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.0M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • BEALL FA, TAYLOR MJ, THORNE CB. Rapid lethal effect in rats of a third component found upon fractionating the toxin of Bacillus anthracis. J Bacteriol. 1962 Jun;83:1274–1280. [PMC free article] [PubMed]
  • Brachman PS, Gold H, Plotkin SA, Fekety FR, Werrin M, Ingraham NR. Field Evaluation of a Human Anthrax Vaccine. Am J Public Health Nations Health. 1962 Apr;52(4):632–645. [PubMed]
  • DAVISSON EO, POWELL HM, MACFARLANE JO, HODGSON R, STONE RL, CULBERTSON CG. The preservation of poliomyelitis vaccine with stabilized merthiolate. J Lab Clin Med. 1956 Jan;47(1):8–19. [PubMed]
  • PUZISS M, WRIGHT GG. Studies on immunity in anthrax. VII. Carbohydrate metabolism of Bacillus anthracis in relation to elaboration of protective antigen. J Bacteriol. 1959 Jul;78(1):137–145. [PMC free article] [PubMed]
  • SARGEANT K, STANLEY JL, SMITH H. The serological relationship between purified preparations of factors I and II of the anthrax toxin produced in vivo and in vitro. J Gen Microbiol. 1960 Feb;22:219–228. [PubMed]
  • STANLEY JL, SMITH H. Purification of factor I and recognition of a third factor of the anthrax toxin. J Gen Microbiol. 1961 Sep;26:49–63. [PubMed]
  • STRANGE RE, THORNE CB. Further purification studies on the protective antigen of Bacillus anthracis produced in vitro. J Bacteriol. 1958 Aug;76(2):192–202. [PMC free article] [PubMed]
  • THORNE CB, BELTON FC. An agar-diffusion method for titrating Bacillus anthracis immunizing antigen and its application to a study of antigen production. J Gen Microbiol. 1957 Oct;17(2):505–516. [PubMed]
  • THORNE CB, MOLNAR DM, STRANGE RE. Production of toxin in vitro by Bacillus anthracis and its spearation into two components. J Bacteriol. 1960 Mar;79:450–455. [PMC free article] [PubMed]
  • WRIGHT GG, DUFF JT, FIOCK MA, DEVLIN HB, SODERSTROM RL. Studies on immunity to toxins of Clostridium botulinum. V. Detoxification of purified type A and type B toxins, and the antigenicity of univalent and bivalent aluminium phosphate adsorbed toxoids. J Immunol. 1960 Apr;84:384–389. [PubMed]
  • WRIGHT GG, GREEN TW, KANODE RG., Jr Studies on immunity in anthrax. V. Immunizing activity of alum-precipitated protective antigen. J Immunol. 1954 Dec;73(6):387–391. [PubMed]
  • WRIGHT GG, PUZISS M, NEELY WB. Studies on immunity in anthrax. IX. Effect of variations in cultural conditions on elaboration of protective antigen by strains of Bacillus anthracis. J Bacteriol. 1962 Mar;83:515–522. [PMC free article] [PubMed]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)