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J Bacteriol. Jan 1963; 85(1): 230–236.
PMCID: PMC278112
STUDIES ON IMMUNITY IN ANTHRAX X.
Gel-Adsorbed Protective Antigen for Immunization of Man
Milton Puziss and George G. Wright
U.S. Army Biological Laboratories, Fort Detrick, Frederick, Maryland
Abstract
Puziss, Milton (Fort Detrick, Frederick, Md.) and George G. Wright. Studies on immunity in anthrax. X. Gel-adsorbed protective antigen for immunization of man. J. Bacteriol. 85:230–236. 1963.—Protective antigen in filtrates of anaerobic cultures of Bacillus anthracis was not readily precipitable by alum or other aluminum salts, but was adsorbed onto a preformed aluminum hydroxide gel under specific conditions. The adsorbed product was highly effective in immunizing rabbits, guinea pigs, and mice against challenge with virulent B. anthracis spores. Gel-adsorbed anaerobic antigens preserved with 1:10,000 thimerosal were unstable on storage at 4 C; replacement of this preservative with 1:40,000 benzethonium chloride produced a more stable product. Addition of 0.0009% formalin further increased the stability during accelerated aging at 37 C. The final product had high protective activity in animals and was well tolerated in man.
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