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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
J Mol Biol. Author manuscript; available in PMC 2010 August 28.
Published in final edited form as:
PMCID: PMC2779859
NIHMSID: NIHMS127119

Relation of intracellular signal levels and promoter activities in the gal regulon of Escherichia coli

Abstract

Transcription of many genes is regulated by combinations of multiple signals. In Escherichia coli combinatorial control is typical in the case of operons related to utilization of different sugars in the absence of glucose. To understand regulation of the transport and metabolic pathways in the galactose system, we measured activities of the six gal regulon promoters simultaneously, using an in vitro transcription system containing purified components. Input functions were computed based on the experimental measurements. We observed four different shapes of input functions. From the results we can conclude that the structure of the regulatory network is insufficient for the determination of signal integration. It is the actual structure of the promoter and regulatory region, the mechanism of transcription regulation, and the interplay between transcription factors that shape the input function to be suitable for adaptation.

Keywords: transcription, signal integration, regulatory network, gal regulon, computational biology

Introduction

Bacteria sense a wide array of signals (minerals, nutrients, stress signals, etc.). A large class of cellular response systems regulates the flux and concentration of small molecules by controlling transport and metabolism pathways via two feedback loops connected by a common transcription factor (TF) that senses the intracellular concentration of the small molecule1. The simplest systems consist of two operons, a regulator gene and a regulated operon containing at least two cistrons, one encoding a transporter and the other encoding an enzyme which modifies/degrades the small molecule2. In E. coli cells expression of several sugar operons are repressed when glucose is abundant in the growth medium. Full expression of these operons occurs when the glucose level is low and the corresponding sugar level is high3; 4; 5; 6. Therefore, regulation of these operons occurs by “combinatorial control”, which is common in both prokaryotes and eukaryotes7; 8. In case of the above sugar operons the combinatorial control is implemented molecularly by a global (CRP) and a sugar specific transcription factor. Activation by CRP requires the inducer cAMP, which is produced intracellularly as a signal of glucose shortage9. In the simplest systems a single operon contains genes encoding a transporter and the enzyme for sugar conversion, therefore transport and metabolism pathways are regulated simultaneously2. However, some sugar utilization systems reached higher levels of complexity, e.g. having multiple transporters, regulators, or several enzymes of a metabolic pathway5; 6. One of the best studied examples of the latter is the galactose utilization system. The gal system contains genes involved in the transport (galP, mglBAC) and amphibolic utilization (galETKM) of the sugar D-galactose. Regulation of the gal regulon is governed by two transcription factors, GalR and GalS (Figure 1). GalR and GalS are iso-regulators because of the high similarity in their amino acid sequence (55% identity/ 88% similarity), inducer binding specificity, and DNA binding site recognition6; 10. To understand combinatorial control of the transport and metabolic pathways in the galactose system it is essential to determine how the two signals, cAMP and D-galactose concentrations are integrated at each promoter. The correlation between the levels of input signals and the promoter activity is called the input function.

Figure 1
Effect of transcription factors and small molecules on the activity of the gal regulon promoters. PgalS, PgalP, PmglB, and P1galE are repressed by GalR and regulated by an incoherent feed-forward loop12 involving cAMP-CRP mediated activation and GalS ...

In this study we measured activities of the six gal regulon promoters simultaneously, using an in vitro transcription system containing purified components. Input functions were computed based on the experimental measurements. Four different shapes of input functions were observed, which are in good agreement with our previous Boolean approximation10. We computed probabilities of operator occupancy by GalR and GalS at different levels of input signals at all six promoters. GalR binding dominates at most of the reported physiological intracellular concentrations of input signals.

Results

Effect of TF and input signal concentrations on gal regulon promoter activities

We studied the effect of varying concentrations of GalR, GalS, D-galactose, and cAMP-CRP on the gal regulon promoters simultaneously using the pRPGSM plasmid 10 in in vitro transcription reactions (Figure 2). The pRPGSM plasmid contains the respective cis-regulatory regions and promoters of the galETKM, galP, galS, galR and mglBAC operons. Reactions contained 80 nM HU protein required for GalR mediated DNA loop formation. RNA products of the in vitro transcription reactions were separated on 7% polyacrylamide-urea DNA sequencing gels. Relative promoter activities were determined based on the level of 32P radioactivity in the separated RNA bands as described in the Materials and Methods section.

Figure 2
In vitro transcription of the gal regulon promoters in the presence or absence of varying amounts of GalR, GalS, CRP, D-galactose and cAMP. cAMP was used at 100 μM concentration when not indicated. HU was present at 80 nM in all reactions to assist ...

Strength of GalR and GalS binding

We constructed a model of the in-vitro system (see Materials and Methods). By fitting this model to our data, we obtain estimates of all binding strengths. The full results are shown in Table 1, but to summarize:

  1. GalR binds to operators in decreasing order of strength: OgalP>galE OE (loop) > OgalR > galS OE > galE OE (no loop) > OmglB
  2. GalS binds to operators in decreasing order of strength: galS OE > OmglB > OgalR > galE OE > OgalP
  3. The Hill coefficients are all around 2 (the range is 1.7 to 2.5).
  4. D-galactose binds slightly stronger to GalR than to GalS, and the Hill coefficient is in both cases a little under 1.
  5. cAMP-CRP binds to the activator binding sites (AS) in the regulatory region of the gal regulon promoters in decreasing order of strength: galP>mglB> galE >galS, and the Hill coefficients lie in the range of 1 to 2.
Table 1
Parameter values in the theoretical model of the gal system. The table shows the 36 parameters that were determined by least-square fitting to in-vitro data. Uncertainties in these values were determined by repeating the fits 50 times, each time with ...

Intracellular promoter input functions

In a cell, the levels of GalR and GalS are not constant. We can use the binding strengths determined above to estimate how promoter activities depend on the intracellular levels of D-galactose and cAMP by describing the dynamics of the GalR and GalS levels using the following differential equations:

dRtotdt=νAgalRR(Rtot,Stot,g,c)-Rtot
(1)
dStotdt=νAgalSS(Rtot,Stot,g,c)-Stot
(2)

where AgalR and AgalS are the promoter activities, defined in Materials and Methods. νR and νS are maximal production rates of GalR and GalS respectively. From the in vivo observation that in the absence of GalR, P1galE is repressed to 43% of its maximal activity, we previously determined that νS=484 nM/cell generation 11. Similarly, in the absence of GalS, P1galE is repressed to 10% of its maximal level, which leads to νR =125 nM/cell generation. We measure time in units of one cell generation (we assume that GalR and GalS are both stable, so the only way their concentrations can decrease is by dilution due to cell growth.)

Figure 3 shows the steady-state activity of various promoters as a function of D-galactose (g) and cAMP (c) concentrations. We observe four different shapes of input functions. Four of the input functions have simple shapes resembling Boolean AND (PgalS, PgalP, and P1galE) or DGAL nimplies CAMP (P2galE) logic. The two other input functions have more complex shapes that can be best approximated by the combination of CAMP and AND (PmglB) logic or DGAL and TRUE (PgalR). The input functions are generally monotonic; however, the PmglB transcription shows non-monotonic dependence on cAMP concentration at low galactose levels.

Figure 3
Promoter activities as a function of intracellular galactose and cAMP levels (z-axis values are relative to PgalR, for which the maximum activity level is chosen to be 1). For each value of cAMP and D-gal, we integrated the differential equations (1) ...

This non-monotonic response is the result of two regulatory features, (i) partial repression of PmglB by GalR10 and (ii) the indirect repression of PmglB by cAMP-CRP through GalS12. The color coding in Figure 3 indicates which of the two regulators dominates (i.e., when the operator is bound by a repressor, what fraction of the time is it GalR) – brown means GalR, blue means GalS. In the case of mgl the dominant repressor changes from GalR, at low cAMP, to GalS, at high cAMP. As cAMP is increased from zero, GalS starts competing with GalR for the OmglB operator, but as GalS is a weaker repressor the net repression decreases. As cAMP is increased further, free GalS levels rise much higher (see Figure 4) once again increasing repression of the mglBAC operon.

Figure 4
Concentration of (a) free GalR (i.e., not bound to either operators or galactose) and (b) free GalS, derived from the theoretical model, in steady-state conditions, as a function of intracellular galactose and cAMP levels.

The color coding for dominance in Figure 3 shows that GalS is mainly responsible for self-repression and repression of PmglB at high cAMP. For the rest, blue-regions are present only when activity levels are close to maximum (i.e., neither repressor spends much time bound to the respective operators). In other words, GalS plays little role in the steady-state repression of the gal regulon. GalR appears to be the main steady-state regulator10.

Functional significance of feed-forward loops in the galactose system

Data presented in Figure 3 allows for the prediction of the functionality of feed-forward loops in the galactose network. In the feed-forward loop network motif a transcription factor regulates a gene both directly and indirectly through regulating another TF. There are two types of feed-forward loops in the galactose network of E. coli, incoherent type I1 and incoherent type I2. In the type I1 feed-forward loops cAMP-CRP activates the target gene directly and represses it indirectly through the activation of PgalS. The significance of type I1 feed-forward loops in the galactose system have been discussed earlier10; 12. In the type I2 feed-forward loops one of the repressors (GalR or GalS) represses a target gene directly and also inhibits repression of the target gene indirectly by repressing the transcription of the other repressor gene. However, these type I2 feed-forward loops can function only at medium to high cAMP concentrations that allow for sufficient expression of GalS. Type I2 feed-forward loops where GalR regulates target genes directly and indirectly through repression of PgalS are mostly inactive because GalS autoregulation dominates at higher cAMP concentrations. There is a narrow range of cAMP concentration where PgalS regulation is dominated by GalR and therefore GalS mediated repression of PmglB is inhibited. This cAMP concentration range overlaps with the non-monotonous region of the input function for PmglB. Type I2 feed-forward loops where GalS represses target genes directly and regulate them indirectly through repression of PgalR are functional at high cAMP levels, where repression of PgalR is strongly affected by GalS. These type I2 feed-forward loops result in slightly increased activity of the PmglB, PgalP, and P1galE promoters at higher cAMP levels.

Discussion

Regulation of gene expression is a main component in the response to the changing environment13. Transcription regulatory networks continuously sense a set of environmental signals and perform computations to adjust the gene expression profile according to the changes of these signals, in order to enhance the cells performance. Transcription of many genes is influenced by multiple signals14, therefore it is essential to understand the relation of promoter activities and combinations of signal levels. The simplest case of combinatorial control is when the promoter activity depends on only two signals. This is typical in case of operons related to utilization of different sugars in the absence of glucose. In this work we analyzed transcriptional regulation of all six promoters of the galactose regulon in E. coli. This study presents the first characterization of signal integration at all promoters in a regulon.

The negative autoregulatory motif at PgalR, which is affected by a single signal (D-galactose concentration), can itself determine the shape of the input function. However, this is not the case when two or more signals are integrated at a promoter. The network structures around PgalP, and PmglB, which control the two galactose transporters, are identical (Figure 1)10, however, the input functions have different characteristics. Network motifs in these cases are insufficient for the determination of signal integration. It is the actual structure of the promoter and regulatory region, the mechanism of transcription regulation, and the interplay between transcription factors that shapes the input function to be suitable for adaptation. Our results support the conclusion of theoretical studies that network motif structure does not determine function15; 16.

The response range of the input function to cAMP and D-galactose concentrations shows good agreement with the potential physiological range of these input signals. The galactose transport system is able to accumulate up to 60 mM D-galactose (nearly 5% of total cell dry weight) when D-galactose is not metabolized intracellularly17. However, wild type cells growing in the presence of D-galactose as a single carbon source contain only about 0.38 mM D-galactose as a net effect of the feedback loops involving transport and metabolism18. As a comparison, input functions show saturation (reach 90% of maximal activity) at maximum 16mM concentration of D-galactose. cAMP is synthesized intracellulary by adenylate cyclase as a response to glucose starvation9. A sharp rise in intracellular cAMP level occurs when the nutrient concentration in minimal medium drops to approximately 0.3 mM glucose19. The level of intracellular cAMP, which is highly unstable19, was reported to reach a maximum of 40−240 μM, depending on growth conditions19; 20; 21. Input functions of the galactose regulon promoters show saturation (reach 90% of maximal activity) at maximum 28μM concentration of cAMP.

We analyzed the effect of the two galactose specific regulators, GalR and GalS, on the repression of the gal regulon promoters at steady state concentrations of D-galactose and cAMP. The strong autoregulation of GalS limits cross-regulation between GalS and GalR by favoring GalS binding to the galS OE operator and by preventing accumulation of GalS in sufficient amount to repress the PgalR promoter. Besides autoregulation, GalS also influence transcription of PmglB at low D-galactose and high cAMP concentrations, however, to much less extent.

Input functions obtained in this study show significant differences from the ones observed by controlling extracellular cAMP and D-galactose levels22; 23. There are several factors that can cause these differences. The relation of extracellular and intracellular concentrations of small molecules is often non-linear partly because of regulated transport and intracellular conversion1. This is particularly true for cAMP24, which is normally synthesized intracellularly, and for small molecules whose transport depends on the absence or presence of other small molecules in the environment. For example, transport of D-galactose is inhibited in the presence of glucose (inducer exclusion)25. Also, transcription of chromosomal transport and metabolic genes is influenced by the number and quality of TF binding sites supplied on plasmids26 in the above in vivo measurements. For instance, supplying a strong GalR binding site (e.g. OgalP) in multicopy would inhibit GalR mediated repression of all the gal regulon promoters, while using a weaker binding site (e.g. OmglB) in a similar setup would be less effective in derepression of promoters with a strong GalR binding site. Therefore contradictions in results observed by the in vivo and in vitro approaches can reveal important aspects of signal transport/ processing.

Materials and Methods

Protein purification

Hexahistidine tagged GalR and GalS was purified as described earlier 27. Briefly, harvested cells were resuspended in 1/40 volume of Lysis I buffer (50 mM sodium phosphate, pH 8.0, 0.5 mg/ml lysozyme) and stored on ice for 30 minutes. Equal volume of Lysis II buffer (50 mM sodium phosphate, pH 8.0, 2 M NaCl, 8 mM imidazole, 20% glycerol) was added and incubated for 30 minutes on ice. The cell debris was removed by centrifugation at 10,000 g for 1 hour. 3% Ni-NTA slurry (Qiagen) was added to the solution, followed by 1 hour incubation at 4°C. A Poly-Prep Chromatography Column (BIO-RAD) was used to collect the protein bound to Ni-NTA agarose from the mixture. Twenty column-volume of washing buffer (50 mM sodium phosphate, pH 8.0, 600 mM NaCl, 60 mM imidazole, and 10% glycerol) was allowed to flow through the column. Hexahistidine tagged proteins were eluted by four column volumes of elution buffer (50 mM sodium phosphate, pH 8.0, 600 mM NaCl, 10% glycerol) containing 250 mM imidazole and fractions containing repressor protein with less than 5% contamination were stored at −80°C in 100 μl aliquots. HU protein was purified according to the method described by Aki et al 28. CRP was purified as described by Ryu et al 29.

In vitro transcription

Transcription reactions were performed as described previously 30. The reaction mixture (50 μl) contained 20 mM Tris acetate, pH 7.8, 10 mM magnesium acetate, 200 mM potassium glutamate, 100 μM cAMP, and 2 nM supercoiled pRPGSM plasmid10 DNA template. GalR, GalS, and CRP concentrations are as indicated in Figure 2. HU was used at 80 nM concentration. 20 nM RNA polymerase (USB) was added before incubating the reactions at 37°C for 5 minutes. Transcription was started by the addition of 1.0 mM ATP, 0.1 mM GTP, 0.1 mM CTP, 0.01 mM UTP, and 5 μCi of [α-32P]UTP (3000 Ci/mmol). Reactions were terminated after 10 minutes by addition of an equal volume of transcription loading buffer (0.025% bromophenol blue, 0.025% xylene cyanol, 0.01 M EDTA and 90% deionized formamide). After heating at 90°C for 3 minutes, the samples were loaded onto 7% polyacrylamide-urea DNA sequencing gels. RNA bands were quantified using the ImageQuant™ PhosphorImager (Molecular Dynamics CA). Band intensities were corrected by the background and normalized to the RNA1 band intensities of the corresponding lanes.

Model of the in-vitro system

In each in-vitro experiment, there is a fixed amount of GalR (Rtot), GalS (Stot), cAMP (c) and galactose (g), some of which values could be zero. There are five operators at which GalR and GalS compete to bind: OmglB, OgalR, OgalP, galS OE, and galE OE. In addition, GalR can bind to galE OE and OI simultaneously, forming a DNA loop, which GalS cannot do. We use R and S to denote, respectively, the amount of free GalR and GalS, i.e. the amount not bound to the operators or galactose. Similarly, we denote the amount bound to galactose by (Rg) and (Sg). Our model of the promoter activities (A) in the in vitro system in steady-state then consists of the following equations:

AiAi,max=11+(RKr,i)hr,i+(SKs,i)hs,i×(cKc,i)hc,i1+(cKc,i)hc,i

where i=mglB, galS, galP. These equations use standard Michaelis-Menten terms to model activation of promoters by cAMP-CRP, as well as repression by GalR and GalS, whose concentrations appear in additive form in the denominator because GalR and GalS compete for binding to the same operator sites in the mglB, galS and galP cis- regulatory regions.6

AgalRAgalR,max=11+(RKr,galR)hr,galR+((Rg)Kr,galR)hr,galR+(SKs,galR)hs,galR

PgalR activity (Agalr) is independent of cAMP-CRP. It is repressed by GalR and GalS which compete for the same binding site (OglaR)10. Therefore AgalR is modeled similarly to Ai above. The only difference is that we also include the possibility for the GalR-galactose complex to bind to the operator site and repress PgalR. This is included because experimental data shows that even for very large concentrations of galactose, PglaR activity does not reach 100%.

AP1AP1,max=1+0.025(RKr,OE)hr,OE+0.025((Rg)Kr,OE)hr,OE1+(RKr,OE)hr,OE+((Rg)Kr,OE)hr,OE+(RKr,loop)2hr,loop+(SKs,OE)hs,OE×(cKc,OE)hc,OE1+(cKc,OE)hc,OE

This equation for the activity of P1galE models the following facts: P1galE is activated by cAMP-CRP, repressed by GalR or GalS binding to galE OE, and strongly repressed by GalR forming a DNA loop by binding to both galE OE and galE OI30; 31; 32. Similar to PgalR, P1galE activity does not reach 100% at large galactose concentrations32, therefore we include the possibility of the GalR-galactose complex binding to galE OE and repressing the promoter32; 33. However, it is known that the GalR-galactose complex is unable to form the DNA loop therefore we exclude that possibility32.

AP2AP2,basal=1+2(RKr,OE)hr,OE+2((Rg)Kr,OE)hr,OE+(RKr,OE)hr,OE(cKc,P2)hc,P2+((Rg)Kr,OE)hr,OE(cKc,P2)hc,P2+1.2(SKs,OE)hs,OE(1+(RKr,OE)hr,OE+((Rg)Kr,OE)hr,OE+(RKr,loop)2hr,loop+(SKs,OE)hs,OE)×(1+(cKc,P2)hc,P2)

P2galE activity has a more complex regulation. Overall, the above equation models the fact that GalR binding to galE OE activates the promoter two fold, whereas both GalR mediated DNA loop formation and cAMP-CRP binding repress it31; 34.

The levels of GalR and GalS bound to galactose are given by:

(Rg)=R(gKr,g)hr,g
(Sg)=S(gKs,g)hs,g

Finally, the total amounts of GalR and GalS can be split into four pools: free protein, bound to galactose but not to an operator, bound to operator sites, and bound to galactose and to an operator site:

Rtot=R+(Rg)+Rbound+(Rg)bound
Stot=S+(Sg)+Sbound+(Sg)bound

From these equations, given values of Rtot, Stot, g and c, we can determine all promoter activities, and the amounts of GalR and GalS bound to each operator and to galactose. The equations have 49 parameters. 13 of these have been derived directly from measured data, and the rest we determine by least squares fitting to the in-vitro data (see Table 1).

Acknowledgements

We thank our colleagues in the laboratory for various inputs in the project, in particular Thomas Soares and Mofang Liu for purification of HU and CRP, and Takácsné Botond Judit for technical assistance. This research was supported by the Intramural Research Program of the NIH, National Cancer Institute, Center for Cancer Research, by a Marie Curie International Reintegration Grant within the 6th European Community Framework Program, by OTKA PD75496, and by the Danish National Research Foundation. SS is grateful for the János Bolyai fellowship of the Hungarian Academy of Sciences.

Abbreviations

GalR
Gal repressor
GalS
Gal isorepressor
cAMP
cyclic AMP
CRP
cyclic AMP receptor protein
HU
heat unstable protein
TF
transcription factor

Footnotes

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