Focus, Huh7 and Hep3B cells exhibit different amounts of multi-node effects in a JAK inhibitor background. A A matrix of the mean of duplicate measurements of the percent of double positive cells in response to varying concentrations of I-kappa-kinase inhibitor (IKKi) and PI3kinase inhibitor (PI3Ki) in a JAK (3µM) inhibitor background. The compounds are the same as in figure 4. Concentrations are as follows: (IKKi, H=20µM, M=6.66µM, L=2.22µM), (PI3Ki, H=5µM, M=1.66µM, L=0.55µM). B The synergy plots display the fold deviation from predictions based on the assumption of additivity. Briefly, additivity predictions for any double-drugged entry (i,j) were calculated by adding the ith row of the first column (i.e. the singular IKK effect at that concentration) to the first row, jth column (i.e. the singular PI3k effect at that concentration). Then the measured value at the double-drugged entry (i,j) was divided by the additivity prediction. This created a metric that describes synergy versus additivity.