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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
Mol Cell. Author manuscript; available in PMC 2010 April 23.
Published in final edited form as:
Mol Cell. 2009 October 23; 36(2): 302–314.
doi: 10.1016/j.molcel.2009.10.002

Figure 4

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UbcH5 is required for IKK activation by TNFα, but not IL-1β

(A) Tetracycline (Tet) was used to induce the expression of shRNA against both UbcH5b and UbcH5c. After stimulation with GST-TNFα or IL-1β for the indicated time, the activation of IKK was analyzed by immunoblotting with an IκBα antibody or by IKK activity assay. (B) U2OS cells expressing UbcH5 shRNA and RNAi-resistant Flag-UbcH5c (left) or Flag-UbcH5c (C85A; right) were treated with tetracycline for 7 days. The cells were stimulated with GST-TNFα for the indicated time and then IKK activation was measured. IKK activity was quantified using PhosphoImager and the fold activation as compared to unstimulated cells is shown.

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