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Alzheimer’s disease and other tauopathies have recently been clustered with a group of nervous system disorders termed protein misfolding diseases. The common element established between these disorders is their requirement for processing by the chaperone complex. It is now clear that the individual components of the chaperone system, such as Hsp70 and Hsp90, exist in an intricate signaling network that exerts pleiotropic effects on a host of substrates. Therefore, we have endeavored to identify new compounds that can specifically regulate individual components of the chaperone family. Here, we hypothesized that chemical manipulation of Hsp70 ATPase activity, a target that has not previously been pursued, could illuminate an entirely novel pathway towards chaperone-based therapies. Using a newly developed high-throughput screening system, we identified inhibitors and activators of Hsp70 enzymatic activity. Inhibitors led to rapid proteasome-dependent tau degradation in a cell-based model. Conversely, Hsp70 activators preserved tau levels in the same system. Hsp70 inhibition did not result in general protein degradation, nor did it induce a heat shock response. We also found that inhibiting Hsp70 ATPase activity after increasing its expression levels facilitated tau degradation at lower doses, suggesting that we can combine genetic and pharmacologic manipulation of Hsp70 to control the fate of bound substrates. Disease relevance of this strategy was further established when tau levels were rapidly and substantially reduced in brain tissue from tau transgenic mice. These findings reveal an entirely novel path towards therapeutic intervention of tauopathies by inhibition of the previously untargeted ATPase activity of Hsp70.
In Alzheimer’s disease (AD), the accumulation of amyloid plaques composed of Aβ peptide is largely accepted as the pathogenic initiator, leading to intracellular accumulation of the microtubule associated protein tau into tangles (Frautschy et al., 1991; Oddo et al., 2003). However, cognitive dysfunction and neuron loss, both in AD and transgenic mice that accumulate amyloid-type pathology, are critically linked to tau (Braak and Braak, 1991; Mukaetova-Ladinska et al., 2000; Roberson et al.). Moreover, tau pathology is found in approximately 15 other neurodegenerative diseases, some of which are caused by mutations in the tau gene itself (Hardy and Orr, 2006). Thus developing strategies to remove abnormal tau in symptomatic patients may be therapeutically beneficial; however, it is not yet clear which targets are best suited to accomplish this task.
Molecular chaperones, such as heat shock proteins Hsp70 and Hsp90, have been implicated in tau processing (Dou et al., 2003; Petrucelli et al., 2004; Shimura et al., 2004a; Shimura et al., 2004b; Dickey et al., 2006b; Dickey et al., 2006c; Luo et al., 2007; Dickey et al., 2008; Carrettiero et al., 2009). Both Hsp70 and Hsp90 utilize ATP to regulate protein refolding (Slepenkov and Witt, 2002). The details of this mechanism and the effects of nucleotide exchange on Hsp70 structure and function have been established using mutagenesis, combined with structural and biophysical studies (Mayer et al., 2000; Brehmer et al., 2001). Briefly, ATP binding to the nucleotide binding domain (NBD) of Hsp70 allosterically promotes a conformational change that initiates low-affinity contact of a substrate/client with the substrate binding domain (SBD). ATP hydrolysis to ADP then causes an adjacent “lid” to close, facilitating high-affinity (~10-fold increase) substrate binding. When ADP is exchanged for ATP by an accessory nucleotide exchange factor (NEF), the lid opens, freeing the client. Despite recent progress, the mechanisms responsible for substrate fate decision making (i.e. degradation/release versus refolding/retention) are not yet clear and it is not known how Hsp70’s ATPase activity is able to couple substrate affinity to folding outcomes.
In contrast to the extensive work on chemical inhibitors of Hsp90 ATPase function (Neckers, 2002; Dickey et al., 2007; Luo et al., 2007), chemical modulators of Hsp70 have proven more elusive, in part because of its comparatively low intrinsic ATPase (less than 0.2 μmole ATP/μmole/min; Chang et al. 2008). Consequently, less is known about Hsp70 and its roles. However, genetic work in models of protein misfolding disorders has routinely documented strong correlations between Hsp70, not Hsp90, in limiting the progression of disease (Dou et al., 2003; Petrucelli et al., 2004). Using a sensitize, new screening assay that overcomes the challenges of weak ATPase activity (Chang et al., 2008), we identified several, first-generation inhibitors and activators of Hsp70 that have allowed us to explore the relationship between Hsp70 and tau. From these studies, some unexpected results have emerged that may change the way chaperone-based therapeutic approaches are being designed across fields.
Methylthionine (AC), tetramethylthionine (MB), thionin (TN) and myricetin were purchased from Sigma and suspended in dimethylsulfoxide (DMSO). The dihydropyrimidines 115-7c and SW02 were synthesized as described (Wisen et al., 2008). Epoxomicin and 17-AAG were acquired from A.G. Scientific. All clones were in the pcDNA3.1 vector. SiRNAs (Qiagen) were transfected at 20 nM. All antibodies were diluted in 5% NFDM in TBST at 1:1000 with the exception of pS396/S404 tau, which was used at 1:100. Where pTau is indicated, pS396/S404 was the antibody used. PHF1 (pS396/S404 tau) was provided by Dr. Peter Davies. 12E8 (pS262/S356 tau) was provided by Dr. Peter Seubert. The following antibodies were purchased from the company indicated in parentheses; α-synuclein (Cell Signaling), TDP43 (Protein Tech), Hsp70 and HSF1 (Assay Designs), HA (Roche), Actin (Sigma Aldrich), Hsp40 (BD Transduction Labs), Hsp27 and total tau (Santacruz Biotech). All cell lines were maintained according to ATCC guidelines. Stably transfected HeLa cells over-expressing wildtype 4R0N human tau were generated by clonal selection with G418 (Invitrogen).
High throughput screening of ATPase activity was performed as previously described (Chang et al., 2008). Briefly, hHsp70 (1 μM) and a generic J-domain co-chaperone (E. coli DnaJ; 1 μM) were prepared in assay buffer (0.02% Triton X-100, 100 mM Tris-HCl, 20 mM KCl, 6 mM MgCl2, pH 7.4). This solution was aliquoted into 384-well plates with 1 mM ATP and either a query compound (10 μM) or DMSO solvent control. After 3 hr at 37 °C, malachite green reagent was used to detect liberated phosphate and the signal measured on a SpectraMax M5 (Chang et al. 2008). The pilot collection of compounds was comprised of the commercial MS2000 FDA-approved set (Spectrum) and an internally generated, focused collection of dihydropyrimidines (Wisen et al., 2008). The statistical parameters of the screen were similar to those previously reported for the prokaryotic Hsp70, DnaK (Z factor ~ 0.7; S/N > 10; CV ~ 8%). Active compounds were defined as those with at least 30% activity: activators accelerated steady-state ATP hydrolysis at least 30%, while inhibitors reduced this value. This criterion was selected because it represents 3 standard deviations of the DMSO control. Hits were subsequently subject to 8-point dose dependence verification in triplicate and the results analyzed with GraphPad PRISM.
The 15N-1H HSQC-TROSY spectra(Salzmann et al., 1998) of 100 μM 15N labeled human Hsc70(1–381) was collected as previously described (Zuiderweg, 2002). Briefly, a 10x MB stock solution was prepared in the same buffer as the NMR sample. Addition of 1 equivalent of MB caused several resonances corresponding to free Hsc70 to disappear. A second equivalent did not cause additional changes; thus, MB is believed to bind in a 1:1 stoichiometry to Hsc70 with slow release kinetics.
Cells were transfected as previously described (Dickey et al., 2006b; Dickey et al., 2007; Dickey et al., 2008). Cell lysates and immunoprecipitates were analyzed by Western blots (Dickey et al., 2007). Briefly, cells were harvested in either Tris/NaCl buffer containing protease and phosphatase inhibitor cocktails (for co-IP) with sonication or in MPER buffer (Pierce) containing protease and phosphatase inhibitor cocktails without sonication. For sarkosyl- and SDS-insoluble protein analysis, 2 mgs of total protein were pre-cleared at 14,000 ×g for 15 minutes (crude lysates) and soluble (S1) tau consisted of the supernatant from a 15 minute 60,000 ×g spin. Pellets from the S1 spin were resuspended in a TBS/sucrose buffer with PMSF (Dickey et al., 2006c) and these pellets were incubated with either 1% sarkosyl or 2% SDS for 1 hour at 37 °C. Soluble and insoluble materials were analyzed by Western blot as previously described (37).
HEK cells transfected with wildtype Hsp70 were lysed in assay buffer (0.017% Triton X-100, 100mM Tris-HCL, 20mM KCl and 6 mM MgCl2, pH 7.4), with 15 strokes of a Dounce Homogenizer B. Cell lysates was supplemented with 0.5 μg purified recombinant His-tagged human 4R Tau and treated with and without 0.5 mM ATP-γS in 500μl total volume for 30 minutes at 37°C with gentle rocking. 20 μl of samples were removed for whole lysate measures. 20μl of Ni-NT Aagarose (Qiagen) were added for pull down co-precipitation of His-tagged tau/Hsp70 complexes. Samples were analyzed by western blot.
Brain slices were prepared from 4 month old rTg4510 mice and wild type (NonTg) littermates as previously described (Mirnikjoo et al., 2001) and treated with MB and AC. Briefly, mice were decapitated and brains were removed and dropped in ice-cold cutting solution. Brains were cut mid-saggittaly and were sectioned horizontally in ice-cold, oxygenated cutting solution at 400 um. Sections were immediately transferred to 50:50 (cutting solution: artificial cerebrospinal fluid, ACSF) at 27°C for 10 minutes and then equilibrated in ACSF at 37C for 30 minutes. Sections were then transferred to warm, oxygenated ACSF containing drugs and incubated for 3 hours at 37C. Tissues were mechanically homogenized and analyzed by Western. Stereotaxic injections of AC or vehicle into eight rTg4510 mice were performed as previously described (Wilcock et al., 2006). Briefly, bilateral hippocampal injections of 2 μL of either a 10 mM solution of AC in 0.9% saline or 0.9% saline only were performed using a stereotaxic rig and a 10 μL Hamilton syringe. Mice were killed 24 hours after injection and brains were harvested and analyzed by Western blot.
Replicate densitometric values were obtained from repeated experiments, Western blots and densitometric variability. For replicate blots/experiments, standard deviations were calculated. For analyses across multiple models and tau species, as well as compilation of Hsp70 inhibitors, standard error of the mean was calculated where the n was ≥ 3. Statistical significance was determined with a heteroscedastic two-tailed Student t-test.
Using a newly described high throughput screening (HTS) method, we evaluated a library of 2,800 known bioactive compounds and identified five active compounds (0.2% hit rate) belonging to at least three chemical scaffolds (Chang et al., 2008); Fig 1A). These compounds either accelerated or reduced the ATPase activity of human Hsp70. The benzothiazine, methylene blue (MB), its demethylated analogue, azure C (AC (McKamey and Spitznagle, 1975)), and the flavonol, myricetin (MY), inhibited human Hsp70 by more than 80% with IC50 values of 83, 11 and 12 μM, respectively (Fig 1B-D). We were also able to identify activators of ATPase activity belonging to the dihydropyrimidine family (115-7c and SW02) that increased Hsp70 function by ~ 45% with EC50 values of ~ 120 to 150 μM. A direct interaction between MB and Hsc70, binding was confirmed by NMR spectroscopy (Pellecchia et al., 2000). We estimated a binding affinity of ~ 1 μM from several well-defined resonances in the HSQC-TROSY spectrum of Hsc70’s nucleotide-binding domain (Fig 1E). Together, these experiments provided a suite of chemical probes, which we hypothesized, could be used to tune chaperone function.
We then explored the role of Hsp70 in HeLa cells over-expressing human tau using these compounds. MB and AC significantly reduced both total tau and phosphorylated tau (pTau), while treatment with 115-7c or SW02 led to accumulation of these species (Fig 2A). Quantification of the blots confirmed that the inhibitors significantly decreased total tau levels at 50 μM (Fig 2B), while stimulating Hsp70 led to an increase of approximately 250–300% in total tau at 50μM (Fig 2B). Thus, molecules identified as inhibitors of ATPase activity led to dose-dependent tau degradation (Fig 2C) and activators caused its levels to increase. When inhibitors were combined and analyzed in aggregate, significant reductions in tau levels were observed in cells treated with 12.5μM or more of inhibitor (Fig 2D). We then analyzed the effects of MB and AC across a number (6 each) of independent experiments to determine the consistency of these data and found highly significant tau reductions at 50μM concentrations of each compound (Fig 2E). Moreover, AC and MB were not significantly different from each other.
We next tested the response of two other disease-associated proteins to gauge the selectivity of these inhibitors for tau. We investigated α-synuclein, which is also an unstructured protein, but accumulates in Parkinson’s disease, and we investigated TAR-DNA binding protein (TARDBP or TDP-43) that accumulates in a number of neurodegenerative diseases and has been identified as a cause for amyolateral sclerosis (ALS; (Josephs et al., 2008; Rutherford et al., 2008; Winton et al., 2008). We over-expressed both of these proteins in the same HeLa cell line that was used to study tau and treated with Hsp70 modifiers. Neither protein was sensitive to Hsp70 inhibitors (MB, AC or myricetin), Hsp70 activators (SW02 or 115-7c), or the Hsp90 inhibitor, 17-AAG (Fig 2F & S1). Although the underlying mechanisms are not yet clear, these findings suggested that tau is particularly sensitive to inhibition of Hsp70’s ATPase activity.
Based on previous biophysical studies of Hsp70 structure and function, we expected that the observed changes in tau stability might be linked to changes in Hsp70’s nucleotide state and subsequent changes in tau binding affinity. Consistent with this idea, addition of a non-hydrolyzable nucleotide analog, ATP-γS, reduced affinity of Hsp70 for tau in a co-precipitation assay (Fig 3A). Moreover, co-immunoprecipitation from cells treated with Veh or AC +/− the proteasomal inhibitor, epoxomicin, showed that fewer Hsp70/tau complexes were present following AC treatment; however ubiquitination of tau was increased (Figs 3B and S2). We also found that epoxomicin abrogated AC-mediated tau reductions (Fig 3C). This is consistent with previous genetic data showing that ATP-bound Hsp70 has a lower affinity for clients. These results also demonstrate that once clients are released from the low-affinity Hsp70/ATP complex, they are delivered to the proteasome for ubiquitination. Although these results all suggested that tau is principally removed by the rapid function of the UPS or other chaperone-mediated degradation pathways, another possibility was migration of tau into an insoluble or aggregated structure (Berger et al., 2007). We therefore performed both traditional sarkosyl and SDS extractions of cell lysates treated with these compounds and found no insoluble tau species (Fig 3D). Thus, while other routes of cellular clearance may contribute to tau reductions following Hsp70 inhibition, conversion into an insoluble material is not facilitated.
Genetic evidence has previously suggested that increasing Hsp70 levels leads to increased binding to tau, which was necessary for CHIP-mediated ubiquitination and degradation (Petrucelli et al., 2004). It has also been shown that Hsp70 over-expression restores tau’s association with the microtubules and preserves tau levels (Dou et al., 2003). Thus, to explore whether changes in tau levels are a result of changes in ATPase activity or changes in absolute Hsp70 levels, we measured chaperone induction in response to inhibitors and activators. Using Western blots against the stress-inducible Hsp70, we found that pharmacological manipulation did not increase expression levels (Fig 4A). In fact, unlike cells treated with the Hsp90 inhibitor, 17-AAG, Hsp70 levels were actually slightly decreased following MB and MY treatment after 24 hours, while expression of other heat shock proteins were unchanged relative to control (Fig 4B). Moreover, knockdown of HSF1 by siRNA did not abrogate the activity of MB or AC (Fig 4C). While a cursory overview of these data would suggest that Hsp70 inhibitors were reducing Hsp70 levels, statistical analysis across multiple experiments revealed a trend but significance was not achieved (p = 0.108). Together, these results suggest that targeting the ATPase activity of Hsp70 controls tau accumulation independent of a global stress response.
Because the Hsp70 inhibitors did not increase chaperone levels, we wondered whether the levels of this protein might be limiting for compound-mediated reductions in tau. To test this idea, cells were transiently transfected with myc-tagged Hsp70 and tau reductions by AC and MB were quantified (Fig 5A). We found that increasing the levels of Hsp70 dramatically enhance the effectiveness of both AC and MB (tau levels were reduced 50% by only 12.5 μM inhibitor). Interestingly, myc-Hsp70 over-expression alone only minimally affected tau levels (Fig 5A). When analyzed in aggregate, Hsp70 inhibitors at concentrations ≥ 12.5μM were significantly more effective at clearing tau when Hsp70 was over-expressed (Fig 5B). These findings suggest that the pool of Hsp70 is limiting for tau degradation in this specific model. Moreover, these studies suggest cells that express high levels of Hsp70, such as stressed cells, might be more sensitive to pharmacologic targeting of Hsp70. To explicitly investigate this possibility, we treated cells with celastrol, a compound that has previously been reported to robustly induce expression levels of Hsp70 (Westerheide et al., 2004). HeLa cells treated for 24 hours with celasterol upregulated their Hsp70 approximately 2- to 3-fold and these cells became sensitive to normally sub-effective doses of MB (Fig 5C; 1 μM reduced tau levels 25%). Thus, by transiently increasing Hsp70 levels, we appeared to have produced more Hsp70/tau complexes that become sensitive to changes in nucleotide turnover. The emphasis here was intentionally placed on “transient” up-regulation of Hsp70 levels, since a concern with current chaperone-based therapies is that they induce heat shock protein expression (Zou et al., 1998). Certainly while transient up-regulation of these proteins may be beneficial, long-term changes in gene expression elicited by compounds would likely have deleterious consequences during a chronic therapeutic course.
Because compounds that directly regulate ATPase function and multi-protein complexes often exert their effect quickly (Jarv et al., 1980; Ward and O’Brian, 1992; Davidson and Varhol, 1995), we anticipated that tau reductions might be quite rapid. Indeed, we found that the rate of tau reduction in a stably transfected cell line was extremely fast, showing reductions within 5 minutes of treatment, consistent with acute enzymatic inhibition (Fig 6A–B). This decline continued over the hour (Fig 6A–B). Rapid reductions were also observed for endogenous tau levels in both human (BE(2)-M17 and SHSY5Y) and murine (Neuro2a) neuroblastoma cell lines that do not over-express tau (Fig 6C). Analysis of drug efficacy across cell lines demonstrated significant decreases in tau levels after 5 minutes following Hsp70 inhibitor treatment, and highly significant reductions after 60 minutes (Fig 6D). Moreover, the flavonol, myricetin (MY), also rapidly reduced tau levels, with reductions evident as early as 1 hour (Fig 6E). Interestingly, tau levels began to rebound after 6 hours, indicative of the highly unstable nature of flavonoid compounds (Boulton et al., 1999). These data gave us further confidence that the compounds might be directly inhibiting Hsp70 activity and facilitating chaperone-mediated tau turnover.
Since the major body of work describing tau dysfunction in AD focuses on its hyper-phosphorylation, we investigated whether over-expression of two kinases that are known to promote tau hyperphosphorylation at distinct residues could affect Hsp70 inhibitor efficacy toward tau. HeLa cells stably over-expressing human wildtype tau were transfected with either glycogen synthase kinase 3β (GSK3β) or microtubule affinity regulating kinase 2 (MARK2/Par1) for 24 hours. These cells were then treated with MB, AC or SW02 for 1 hour and harvested. Tau phosphorylated at S396/S404 was significantly reduced with the inhibitors, regardless of which kinase was over-expressed (Fig 7A). Interestingly, in cells over-expressing MARK2/Par1, tau phosphorylated at S262/S356 was not affected by the inhibitors, consistent with previous data suggesting that MARK2/Par1 can essentially make tau chaperone resistant (Dickey et al., 2006b; Dickey et al., 2007) (Fig 7A). Total tau levels were also reduced in all cases. SW02 did not increase tau levels following the short 1 hour incubation. Next, using three tau mutants that are known to cause fronto-temporal dementia with parkinsonism linked to chromosome 17 (FTDP17) or progressive supranuclear palsy (PSP), we investigated whether Hsp70 inhibition could facilitate their clearance similar to wildtype tau, also drawing a comparison with Hsp90 inhibition. The tau mutants P301L, V337M and R406W were all significantly reduced following inhibitor treatment, similar to wildtype levels (Fig 7B). Treating cells with the Hsp90 inhibitor, 17-AAG, caused similar reductions in wt and P301L tau after 24 hours; however V337M and R406W tau were less efficiently cleared (Fig 7B). When all 4 tau variants were analyzed in aggregate, only Hsp70 inhibition significantly reduced tau levels (Fig 7B). These findings confirm a difference in the mode-of-action of Hsp90 and Hsp70 inhibitors and they suggest that some tau mutants might be handled by distinct components of the chaperone system.
Fortunately, some of the Hsp70 inhibitors (MB and AC) we identified have well-characterized pharmacological characteristics (e.g. stability, lifetime), which we reasoned could facilitate their use in disease models. To take advantage of this property and further explore the potential of Hsp70 as a therapeutic target, we investigated the impact of MB and AC on tau levels in organotypic brain slices from mice engineered to express mutant human tau (rTg4510) and age-matched wild type littermates. In both tissue types, tau levels were significantly reduced following three hours of treatment with either MB or AC (50μM; Fig 8A). Dramatic reductions in one pTau isoform linked to Alzheimer’s disease, pS396/S404, were also evident in the rTg4510 model. Thus, non-dividing neurons also realized rapid reductions in tau following Hsp70 inhibition. We then directly injected AC (2 μL of 10 mM) or saline into the hippocampi of eight 4 month-old rTg4510 mice and harvested these animals 24 hours after surgery. Remarkably, these studies revealed that AC significantly and uniformly reduced soluble tau and pS396/S404 tau levels in the hippocampus (Fig 8B). Together, these studies show that inhibitors of Hsp70 potently stimulate clearance of both normal and abnormal tau in brain tissue.
Based on a growing body of evidence demonstrating a role for Hsp70 in modulating the stability of tau in AD, we endeavored to identify small molecules that could directly modulate Hsp70 ATPase activity as a potential therapeutic strategy for AD. We originally hypothesized that activation of Hsp70 ATPase activity would lead to tau clearance; however, our results show quite the opposite. Inhibiting Hsp70 activity dramatically reduced tau levels, while activating it actually preserved tau levels in the cell. This paradoxical finding raised an interesting possibility; that as Hsp70 levels increase during a stress event, more Hsp70 is available to bind to unfolded substrates that result from the stress event. In tauopathies, where the accumulation of tau is gradual over time, a rapid increase in Hsp70 levels would likely lead to a reduction in tau levels simply based on stoichiometric principles; more Hsp70 is available to bind tau, decide tau’s fate, and facilitate its retention or clearance. Even if a small fraction of tau is cleared by Hsp70 normally, increasing Hsp70 expression will lead to a perceived reduction in tau simply due to the increased number of Hsp70/tau complexes. Here, we show that with these new ATPase inhibitors, we can dramatically increase the fraction of Hsp70-bound tau that is cleared in circumstances where Hsp70 levels are artificially increased (Fig 9). This explains how increasing Hsp70 levels or inhibiting its ATPase activity directly can lead to the same result; reductions in tau.
This principle highlights the most intriguing clinical aspect of this work; that transient up-regulation of Hsp70 expression followed by inhibition of its ATPase activity could prove more efficacious for the treatment of AD and other tauopathies. As Hsp70 levels increase, it binds more tau. If we then inhibit its ATPase activity, we block its attempts to re-fold tau and force all of the bound tau to be released to the UPS, or other chaperone-mediated degradation pathways, for degradation. Thus, bursts of Hsp70 up-regulation, perhaps by celastrol administration (Westerheide et al., 2004), followed by inhibition of its ATPase activity could prove a much more effective strategy in protein folding diseases compared with chronically enhancing either alone, or even together. This strategy would make a chemotherapeutic approach that targets the chaperones in AD and other neurodegenerative diseases more feasible, given the potential for side effects of drugs targeting central signaling complexes such as the Hsp70 and Hsp90 scaffolds.
In this same vein, a current concern with Hsp90 inhibitors is that they induce expression of other heat shock proteins. This is due to the role of Hsp90 as a repressor of HSF1 (heat shock factor 1), the major transcription factor for heat shock proteins. Once Hsp90 is inhibited, HSF1 is freed and can begin transcribing de novo heat shock proteins (Zou et al., 1998; Dickey et al., 2005). Certainly this can be viewed as either a two-pronged attack or a double-edged sword. HSF1 activation has been shown to have positive consequences in some systems of neurodegenerative disease modeling; however, chronic up-regulation of heat shock proteins is likely deleterious at some level, particularly in such long-coursed diseases as AD and Huntington’s disease. Because of this we speculated whether inhibiting Hsp70 at physiological levels also had a feedback consequence that regulated heat shock protein expression, perhaps providing an alternative to Hsp90 inhibition. We found that levels of heat shock proteins were not elevated following inhibition; in fact they were marginally decreased, suggesting that inhibiting Hsp70 activity promotes its clearance as well. Thus, intermittent therapy with Hsp90 inhibitors followed by Hsp70 inhibitors could prove to be a highly effective drug regimen for treating AD and other chronic diseases of protein misfolding.
An interesting coincidence of these data was our previous work identifying MB from a cell-based screening assay as a potent reducer of endogenous tau levels (Dickey et al., 2006a) and the subsequent discovery that MB was also capable of inhibiting Hsp70 ATPase function. Prior to the latter discovery, we described MB as an aggregation inhibitor, based on previous findings (Wischik et al., 1996). In fact, its efficacy as an aggregation inhibitor was what propelled AC, the de-methylated analogue of MB (McKamey and Spitznagle, 1975), to clinical trials as the first tau-based therapy to reach patients. In light of the novel context that MB and AC inhibit Hsp70 activity, their ability to reduce endogenous tau levels has been revealed. Thus the convergence of data from two screens serendipitously led to the independent validation for each.
These compounds may also help us to better understand the role of chaperones in regulating normal tau versus pathological tau. Previous work shows that Hsp70 actually functions to enhance the association of tau with microtubules (Dou et al., 2003). Here, Hsp70 activators appear to preserve tau levels, while Hsp70 inhibitors clear tau. This would coincide nicely with a model where Hsp70 tries to protect tau from degradation, but may actually become too efficient at it, preserving even toxic tau species.
One final consideration when exploring the clinical potential of this strategy is that Hsp70 is involved in the processing of multiple substrates, especially those that are misfolded (Barral et al., 2004; Bukau et al., 2006; Balch et al., 2008). We therefore wanted to investigate the specificity of this approach. While we only tested two additional substrates that also accumulate in neurodegenerative diseases, α-synuclein in Parkinson’s disease, and TAR DNA binding protein (TARDBP or TDP-43) in amyolateral sclerosis (ALS), neither of these proteins were affected by drug treatment, suggesting a certain degree of selectivity of Hsp70 for tau. Certainly other disease associated proteins that have a well established connection to Hsp70 may be affected by these compounds, such as poly-glutamine expansion diseases and prion diseases; however, the finding of some selectivity could be invaluable to the clinical viability of this strategy for AD.
In conclusion, Hsp70 is a central component of the protein folding and triage machinery, yet the mechanisms it employs to enact cellular decisions and control the fate of substrates are not yet clear. We have identified a battery of new chemical modulators of Hsp70 and used these compounds to probe how the activity of the endogenous chaperone controls tau processing. Based on these studies, we propose a model in which acute inhibition of Hsp70 might be beneficial by triggering rapid, UPS-mediated clearance of tau (Fig 9). Moreover, we propose that the ATP-hydrolysis-driven, pro-folding activities of the chaperone, as favored by the activator molecules, may be counter-productive under some conditions. Together, these results clarify the roles of endogenous Hsp70 in tau processing and suggest a previously unanticipated avenue for therapeutic intervention.
Supplemental Figure 1. Quantitation of synuclein and TDP43 levels from representative blots in Fig 2F reveals no significant changes in protein levels after Hsp70 modulation.
Supplemental Figure 2. Hsp70 inhibitors increase tau ubiquitination, while activators do not. Ubiquitination of tau is evident in immunoprecipitates from HEK tau transfectants following treatment with AC (10 μM), SW02 (10 μM) or vehicle (Veh) for 1hr. General IgG immunoprecipitation controlled for non-specific tau binding.
C.A.D. acknowledges support from the Alzheimer’s Association (NIRG-06-27099), the NIA (AG031291) and the Abe and Irene Pollin Fund for CBD from CurePSP. J.E.G. acknowledges support from NIH/NINDS (NS059690). The authors thank Dr. Peter Seubert (Elan Pharmaceuticals) for contributing the 12E8 antibody, Dr. Bingwei Lu for the Par1 expression plasmid, Dr. Benjamin Wolozin for the GSK3β expression plasmid, Dr. Peter Davies (Albert Einstein COM) for the PHF1 antibody and Dr. M. Sherman for providing the Hsp70 expression plasmid.