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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
Am J Med Genet A. Author manuscript; available in PMC 2009 November 9.
Published in final edited form as:
PMCID: PMC2774842

Locus Homogeneity Between Syndactyly Type 1A and Craniosynostosis Philadelphia Type?

To the Editor

In general, classic acrocephalosyndactyly syndromes are characterized by coronal craniosynostosis, particular hand and foot malformations, and facial dysmorphology including hypertelorism, down-slanting palpebral fissures, and midface hypoplasia. The most recently described of these syndromes is craniosynostosis, Philadelphia type, whose distinct clinical manifestations include sagittal craniosynostosis, rather than coronal craniosynostosis, and complete soft-tissue syndactyly of the fingers and toes (Fig. 1) with a relatively normal facial appearance [Robin et al., 1996]. Pedigree analysis of a five generation kindred indicates that the syndrome is inherited in an autosomal dominant fashion, with variable expression of the hand findings, and incomplete penetrance of the sagittal craniosynostosis [Robin et al., 1996]. Previous studies have excluded FGFR1 (implicated in Pfeiffer syndrome), FGFR2 (implicated in Pfeiffer, Apert, Crouzon, and Beare-Stevenson syndromes), FGFR3 (implicated in Muenke syndrome), and FGF8 where a rare 18 bp duplication of exon 1c was identified in two affected members of the family but did not segregate with the trait [Yoshiura et al., 1997; Muenke and Wilkie, 2001]. Without any evidence of previous mutations, we performed linkage analysis on a single family, the only described family with this disorder to date. Since craniosynostosis, Philadelphia type, is a clinically distinct craniosynostosis, with apparently a separate genetic etiology, characterizing the molecular basis for this disorder will point to a novel mechanism important in craniofacial and limb development. Specifically this would be the first gene that is involved primarily in sagittal craniosynostosis.

Fig. 1
Hands and feet of an individual with craniosynostosis Philadelphia type.

We performed linkage analysis using microsatellite markers genotyped at deCODE genetics (1.5 cM average spacing, 2000 markers). For all markers, Mendelian errors were examined using PEDCHECK and when Mendelian errors were detected, the markers were removed from the analysis [O’Connell and Weeks, 1998]. Autosomal parametric and non-parametric linkage analysis was performed using GENEHUNTER-PLUS [Kruglyak et al., 1996; Kong and Cox, 1997]. An autosomal dominant model was tested with the allele frequency of the disease causing mutation set at 0.001. For regions of linkage haplotyping was performed using MERLIN’s-best function [Abecasis et al., 2002]. Haplotypes in regions of linkage were evaluated using HAPLOPAINTER in order to define minimal critical genomic intervals by examining recombination events [Thiele and Nurnberg, 2005].

Parametric linkage analysis identified four suggestive regions of linkage for craniosynostosis Philadelphia type on 2q35-36.3, 12q14.1-21.2, 20q11.2-13.13, and 22q12.1-13.1 with a LOD = 2.40 and multipoint NPL = 8.50. A review of the literature identified an overlapping locus for a phenotypically similar disorder, syndactyly type 1A or complete soft tissue syndactyly of digits two through five [Bosse et al., 2000; Ghadami et al., 2001]. Given the presence of complete soft tissue syndactyly as the major finding in craniosynostosis Philadelphia type, the phenotypic similarity and overlapping regions of linkage for the two disorders suggest they have the same etiology. Syndactyly type 1A has been demonstrated to be inherited in an autosomal dominant fashion with linkage to 2q35 in two families [Bosse et al., 2000; Ghadami et al., 2001]. The minimal critical interval for syndactyly 1A spans from D2S2319 to D2S344 (214.5–221.3 MB) [Bosse et al., 2000]. The overlapping minimal critical interval for craniosynostosis Philadelphia type spans from D2S2361 to D2S2297 (216.2–230.2 MB, Figs. 2 and and3).3). Thus, narrowing the interval using both phenotypes, gives a minimal critical interval spans from 216.2 to 221.3 MB on chromosome 2.

Fig. 2
Linkage results on chromosome 2 indicate a region of linkage to chromosome 2q35-36.3 for craniosynostosis Philadelphia type (CPT; D2S2361 to D2S2297 or 216.2–230.2 MB). This overlaps with a previously described linkage peak for syndactyly 1A (S1A, ...
Fig. 3
Haplotype analysis in the studied pedigree demarcates a minimal critical interval for craniosynostosis Philadelphia type between D2S2361 and D2S2297.

This 5.1 MB interval contains 67 predicted or verified protein coding genes and three microRNAs. Transcripts were prioritized based on known function, expression and/or interactions in the literature. In addition we evaluated a published expression dataset consisting of murine forelimb RNA run on the Affymetrix Murnie Gene Chip (MOE-430) at embryonic day 12.5, just prior to the induction of apoptosis in the mouse inter-digital areas [Shou et al., 2005]. We sequenced coding exons of four protein coding genes (TMBIM1, RQCD1, RNF25, ATG9A) and one microRNA hairpin precursor (hsa-mir-153-1 predicted to regulate FGFR2) [Lewis et al., 2003, 2005] in two individuals with craniosynostosis Philadelphia type and two individuals with syndactyly 1A from the German and Iranian family, respectively. To date, there have been no mutations found co-segregating within families for this disorder; however, we have not excluded the involvement of mutations in untranslated regions or regulatory regions. Future studies will focus on other genes within the minimal critical interval including, IHH, IGFBP2, and IGFBP5.

Although initial linkage results identified four regions of linkage that were completely segregating with the trait, we chose to focus on chromosome 2q35 given the linkage to syndactyly type 1A [Bosse et al., 2000; Ghadami et al., 2001]. The similar limb finding in craniosynostosis, Philadelphia type is consistent with the notion that these disorders share a common etiology [Robin et al., 1996]. The assertion that these two disorders share a common genetic etiology could only be made after linkage analysis narrowed potential regions of linkage for craniosynostosis Philadelphia type.

Syndromic forms of craniosynostosis often manifest limb anomalies as associated findings [Wilkie et al., 2001]. One classic example is Apert syndrome, which is caused by mutations in FGFR2 and is characterized by coronal craniosynostosis and tissue and/or boney syndactyly of the hands and feet [Wilkie et al., 1995]. More recently, a specific mutation in FGFR3 has been shown to cause Muenke syndrome, which is characterized by coronal craniosynostosis and brachydactyly [Muenke et al., 1997]. The documented pleiotropic effect of genes involved in craniofacial and limb development [Wilkie et al., 2001], and the linkage evidence, strongly suggests that sagittal craniosynostosis and syndactyly 1A share a common genetic etiology.


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