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The purpose of the study was to determine the prevalence of appendices with an equivocal appearance at computed tomography (CT) in a control population. We retrospectively identified a control population of 150 patients who underwent CT of the abdomen and pelvis for evaluation of hematuria (without abdominal pain, fever, or colonic disease). One reader measured the diameter of the appendix and noted if the appendix was either isodense in appearance or airless and fluid filled. Sixty-seven of 150 cases (44.6%) demonstrated appendiceal diameter greater than 6 mm. The appendix was collapsed or isodense in 34/150 cases (22.7%). Only ten of 150 or 6.6% of cases were isodense in combination with diameter greater than 6 mm, and none had diameter greater than 10 mm. Only one of 150 cases (0.67%) demonstrated airless fluid within the lumen, and the appendix measured less than 6 mm. While the diameter of the normal appendix is frequently greater than 6 mm, none measured greater than 10 mm in combination with ambiguous morphology. Furthermore, in the normal appendix, airless fluid filling the lumen is a rare appearance with a prevalence of less than 1%. While appendicitis could undoubtedly occur in an isodense appendix between 6 and 10 mm in diameter, such an appearance can occur in up to 6.6% of the normal population
Computed tomography (CT) has become the primary imaging modality for evaluation of adult patients with suspected appendicitis. While there are multiple signs of appendicitis at CT, appendiceal enlargement is considered the most accurate with reported sensitivity and specificity of 92–93% and 92–100%, respectively [1–3]. In most cases, patients with acute appendicitis also demonstrate secondary signs of inflammation at CT that aid in diagnosis. However, appendiceal enlargement is sometimes an isolated finding and has been reported to indicate acute appendicitis in up to 52% of symptomatic patients .
Therefore, knowledge of the parameters of normal appendiceal diameter is critical in suggesting or excluding a diagnosis of appendicitis at CT.
Early studies suggested that an appendix measuring greater than 6 mm was abnormal. This numeric threshold originated in the ultrasound literature [5–8] and was subsequently utilized in CT interpretation. However, the 6-mm threshold assumes a technique of graded compression of the appendix at ultrasound and is therefore difficult to extrapolate to CT interpretation. Prior studies have shown that in up to 42% of patients, the normal appendix measures greater than 6 mm in outer wall-to-outer wall diameter at CT [9, 10]. However, these studies have typically included appendiceal contents, such as air, in measurements of appendiceal diameter. In these instances, the fact that the appendix measures greater than 6 mm is of little clinical value, as a predominantly air-filled appendix lacking secondary signs of appendicitis would always be interpreted as normal at CT. Only normal appendices that are fluid filled or uniformly dense (where the luminal contents cannot be differentiated from the adjacent wall) might realistically be mistaken for appendicitis based on size criteria alone. Therefore, we undertook this retrospective study to determine the prevalence of morphologically equivocal, either fluid-filled or isodense appendices, measuring greater than 6 mm at CT in a control population.
This single-institution study was approved by our Institutional Review Board and was compliant with requirements of the Health Insurance Portability and Accountability Act. The requirement for informed consent was waived. To determine the prevalence of fluid-filled or isodense appendices measuring greater than 6 mm at CT, one author used a computer search to retrospectively identify all multidetector-row CT scans performed for evaluation of hematuria between January 2002 and January 2006. Only the first scan for each patient was included in the review. Electronic medical records were searched for relevant clinical history. Patients were excluded if they reported abdominal pain or fever at the time of imaging, had evidence of colonic wall thickening at CT, or if the appendix was not visualized. The scans of 150 consecutive patients were reviewed. The group included 96 women and 54 men with mean age of 54.7 (range 21 to 94).
All 150 patients in the retrospective review group were scanned with multidetector-row CT (four, 16, or 64 detectors; HighSpeed or LightSpeed, General Electric, Milwaukee, WI, USA) using 1.25 or 5 mm slice thickness. Scans performed at 1.25 mm collimation were reconstructed to 5 mm slice thickness. The peak tube voltage was 120 kVp and the milliampere was automatically adjusted to attain a noise index of 12. For 112 patients, 150 mL intravenous iohexol (Omnipaque 350 Nycomed Amersham, Princeton, NJ, USA) was injected at a rate of 3 to 5 mL/s, and the scan delay was 80 s. Although these patients also had precontrast images performed, when postcontrast scans were available, they were preferentially used for retrospective review. Thirty-three of these patients also received 800 mL oral diatrizoate meglumine (Hypaque, Nycomed Amersham, Princeton, NJ, USA) prior to imaging. Routine administration of positive enteric contrast was only performed at our institution prior to 2006. Thirty-eight patients received neither oral nor intravenous contrast.
One radiologist with 3 years subspecialty experience in abdominal imaging independently reviewed all CT images on a picture archiving and communication system workstation (IMPAX Version 4.5, Agfa, Mortsel, Belgium). In each case, the maximal outer wall-to-outer wall diameter of the appendix was measured to the nearest 0.5 mm, and the average of three measurements was recorded. Measurements were obtained utilizing 200% magnification of axial images for all cases. The single wall thickness of the appendix was also measured and recorded in the same fashion. The predominant type of luminal contents (air, enteric contrast, or fluid) was recorded. An appendix was considered ambiguous in morphology if it had a uniformly collapsed, isodense appearance or contained airless, fluid within its lumen. Because it is difficult to differentiate a collapsed appendix from a distended appendix with contents of the same attenuation as the wall [10, 11], only the outer wall-to-outer wall diameter was recorded when the luminal contents were not visually distinguishable from the wall.
Descriptive analysis was performed using the Stata software package version 8.0 (Stata Corporation, College Station, TX, USA) for Windows (Microsoft, Redmond, WA, USA). The Student t test was used to compare the mean values of outer wall-to-outer wall appendiceal diameter between groups with different luminal contents and those with a collapsed or isodense appearance. In patients in whom the appendiceal wall was discernable, the Student t test was used to compare the mean single wall thickness between groups with different luminal contents. Fisher’s exact test was used to compare the proportions of patients with and without isodense appendices in the groups who received intravenous contrast or enteric contrast versus those who did not. A p value <0.05 was considered to be statistically significant.
The mean outer wall-to-outer wall diameter of the appendix for all 150 retrospectively evaluated CT scans was 6.3 mm (Table 1). Sixty-seven of 150 cases (44.6%) had an outer wall-to-outer wall diameter greater than 6 mm.
Of the 150 CT scans, luminal contents were distinguishable from the appendiceal wall in 116 of 150 cases or 77%. The mean outer wall-to-outer wall diameter and mean single wall thickness for this group are reported in Table 1. The outer wall-to-outer wall diameter varied by type of luminal contents with the greatest mean diameter seen in those containing air, compared to those containing enteric contrast (p=0.01) or fluid (Table 1; Figs. 1 and and2).2). Of note, only 12 of 33 (36%) patients who were given positive oral contrast showed contrast filling the appendiceal lumen at CT. Only one case of 150 (0.67%) had an ambiguous appearance due to airless fluid within the appendiceal lumen, and it measured less than 6 mm in outer wall-to-outer wall diameter (Fig. 3). There was no statistical difference in single wall thickness of the appendix between groups with different luminal contents (p=0.2 to 1.0).
Luminal contents were not discernable from the adjacent wall in 34 of 150 cases (22.7%) resulting in an ambiguous morphology due to a collapsed or isodense appearance of the appendix (Fig. 4). Mean outer wall-to-outer wall diameter for this group is reported in Table 1 and is less than the mean diameter in cases with visible luminal contents (p<0.01).
Only ten of 150 or 6.6% of appendices were isodense and had an outer wall-to-outer wall diameter greater than 6 mm. None had diameter greater than 10 mm. There was no statistically significant difference between the proportion of isodense appendices seen on CT scans performed with (23 of 112 or 21%) versus without intravenous contrast (11 of 38 scans or 29%, p=0.4). Furthermore, there was no statistically significant difference between the proportion of isodense appendices seen on CT scans performed with (12 of 33 or 36%) versus without enteric contrast (22 of 117 or 19%, p>0.05). In fact, the appendix was slightly more likely to appear isodense when enteric contrast was administered.
CT is the most common imaging modality used to evaluate adult patients with suspected appendicitis, with both high sensitivity and specificity for the diagnosis [12–22]. In most cases, CT interpretation is straightforward with cases definitively falling into either positive or negative categories. When appendicitis is present, we typically find both appendiceal enlargement and secondary signs of inflammation. In normal cases, by contrast, the appendix may demonstrate a range of sizes, but no secondary signs of inflammation are usually identified. Unfortunately, as appendiceal enlargement is sometimes the only sign of appendicitis, up to 12% of cases may be equivocal at CT .
As such, it is important for the radiologist to have a clear understanding of the size parameters of the normal appendix at CT. Similar to previous authors [9, 10], we found that 45% of normal appendices have outer wall-to-outer wall diameter greater than 6 mm at CT. However, our study also indicates that in the majority of cases (77%), the appendix had a recognizably normal morphology, either predominant luminal air or enteric contrast. Only 6.6% of normal appendices measured greater than 6 mm and also had a CT appearance that was ambiguous enough to be mistaken for appendicitis at CT, in other words, an isodense, collapsed appearance, or an airless fluid-filled lumen. None had diameter greater than 10 mm in combination with an ambiguous or equivocal morphology.
The pathophysiology of acute appendicitis usually involves appendiceal obstruction with continued mucinous fluid secretion and bacterial proliferation within the lumen of the appendix . As such, a fluid- or mucus-filled appendix is recognized as a sign of acute appendicitis at CT . To our knowledge, the incidence of this appearance in a normal population, however, has not been previously reported. In this study, only 0.67% or one of 150 of control patients demonstrated airless fluid in the appendiceal lumen. A recent study by Moteki and Horikoshi  found that depth of the intraluminal appendiceal fluid greater than 2.6 mm had high sensitivity and specificity (>80%) for diagnosis of acute appendicitis. In our single normal appendix with a fluid-filled lumen, the maximal depth was only 2 mm. In clinical practice, airless fluid is at least occasionally identified in the appendiceal lumen in patients with no clinical evidence for appendicitis, particularly in the setting of a fluid-filled cecum. However, given the rarity of this appearance in the normal appendix and its known association with acute appendicitis, the finding of a fluid-filled appendix with diameter greater than 6 mm should prompt a clinical work up for appendicitis (including blood work analysis and surgical evaluation), even in the absence of secondary signs of inflammation at CT. If the diagnosis remains unclear following clinical correlation, patient observation and short-interval follow-up CT may be appropriate.
Our study has several limitations. The primary limitation is the lack of a standard of reference for proof of a normal appendix. However, pathological correlation is unavailable in this population, as none of the patients included in our study had any clinical evidence of acute appendicitis or colonic disease. Furthermore, the outer wall-to-outer wall diameter of the appendix in our study is concordant with values previously reported in the CT literature for normal appendices [9–11]. Because the focus of our paper is on CT appearance of normal appendices, we also did not include a group of patients with clinically proven appendicitis to evaluate how many might have ambiguous CT findings of isodense or fluid-filled appendices. Another limitation is the retrospective nature of our study. Due to retrospective data collection, all patients were not scanned with intravenous contrast. It is possible that luminal contents may have been identified in more patients with an isodense-appearing appendix if intravenous contrast was administered to all patients. However, there was no statistical difference in the proportion of isodense appendices versus those with visible luminal contents between cases performed with versus without intravenous contrast.
In summary, our study found that while the outer wall-to-outer wall diameter of the normal appendix is frequently greater than 6 mm, none had diameter greater than 10 mm in combination with equivocal morphology. Furthermore, in the normal appendix, airless fluid within the lumen is rarely seen with a prevalence of less than 1%. Therefore, a fluid-filled appendix should alert radiologists and clinicians for the possibility of appendicitis, even in the absence of secondary signs of inflammation at CT. While appendicitis could undoubtedly occur in an isodense appendix measuring between 6 and 10 mm in diameter, such an equivocal appearance can occur in up to 6.6% of the normal population.
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