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Glycoprotein L (gL) is one of four glycoproteins required for the entry of herpes simplex virus (HSV) into cells and for virus-induced cell fusion. This glycoprotein oligomerizes with gH to form a membrane-bound heterodimer but can be secreted when expressed without gH. Twelve unique gL linker-insertion mutants were generated to identify regions critical for gH binding and gH/gL processing and regions essential for cell fusion and viral entry. All gL mutants were detected on the cell surface in the absence of gH, suggesting incomplete cleavage of the signal peptide or the presence of a cell surface receptor for secreted gL. Coexpression with gH enhanced the levels of cell surface gL detected by antibodies for all gL mutants except those that were defective in their interactions with gH. Two insertions into a conserved region of gL abrogated the binding of gL to gH and prevented gH expression on the cell surface. Three other insertions reduced the cell surface expression of gH and/or altered the properties of gH/gL heterodimers. Altered or absent interaction of gL with gH was correlated with reduced or absent cell fusion activity and impaired complementation of virion infectivity. These results identify a conserved domain of gL that is critical for its binding to gH and two noncontiguous regions of gL, one of which contains the conserved domain, that are critical for the gH/gL complex to perform its role in membrane fusion.
Glycoprotein L (gL) is one of the four glycoproteins required for the entry of herpes simplex virus (HSV) into cells and for virus-induced cell fusion (26, 33). The others are gB, gD, and gH (30). The functional unit containing gL is a heterodimer formed with gH (gH/gL) (15). Because mature gL has no membrane-spanning domain, other than a cleavable signal peptide, it is secreted unless it is coexpressed with gH, a type 1 glycoprotein that anchors gL to the cell membrane (2). Also, gH is not properly processed or transported out of the endoplasmic reticulum unless it is coexpressed with gL (15).
Most, if not all, herpesviruses express orthologs of gB, gH, and gL, which are believed to form the core membrane-fusing machinery necessary for viral entry and cell fusion. For some herpesviruses, such as Epstein-Barr virus and human cytomegalovirus, the gH/gL oligomer may contain additional viral subunits that can influence binding of the complex to cell receptors and determine cell tropism (14, 34, 35). For HSV, however, only gD and gB have been shown to have receptor-binding activities that are required for entry (27, 31). Although HSV gH has an RGD motif and the gH/gL heterodimer can bind to certain integrins, this binding seems not to be necessary for viral entry (3, 22).
The initial interaction of HSV with cells can be the reversible attachment of virus to cell surface heparan sulfate, mediated by viral glycoprotein gB and/or gC (29). Then, gD can bind to one of its receptors, including herpesvirus entry mediator (HVEM), a member of the tumor necrosis factor receptor family; nectin-1 or nectin-2, cell adhesion molecules belonging to the immunoglobulin superfamily; or specific sites in heparan sulfate generated by 3-O-sulfotransferases (31). In addition to binding to heparan sulfate, gB can also bind to other cell surface receptors, including paired immunoglobulin-like receptor alpha (PILRα) (27). Binding of both gD and gB to one of their respective receptors appears to be required for triggering the membrane-fusing activity of gB and/or gH/gL, which leads to viral entry.
A recent X-ray structure of HSV type 1 (HSV-1) gB suggests that it is a class III viral fusogen similar in domain organization, but not primary sequence, to the G protein of vesicular stomatitis virus (13). It has been proposed that HSV-1 gH has features characteristic of class I viral fusogens, such as putative heptad repeats and fusion peptides (6, 9-11). Also, peptides matching the sequence of gH can interact with lipids and/or induce the fusion of lipid vesicles (4, 5, 8). Hemifusion between cells and between virus and cell can be induced by gH/gL and gD in the absence of gB (32). Many questions remain about the respective roles of gH/gL and gB in inducing membrane fusion.
The four conserved cysteines in gL were found to be essential for gL-gH association and function (1). Mutational analyses of gL by C-terminal deletions showed that the first 147 amino acids of gL are sufficient for association with gH but that the first 161 amino acids are necessary for cotransport of gH and gL to the cell surface (17, 23) and for gL activity in cell fusion and viral entry (17). Lastly, certain anti-gL monoclonal antibodies (MAbs) can inhibit cell fusion but not viral entry, despite demonstrable binding of the MAbs to virus, suggesting that gL may play a different role in each process (21). These MAbs were mapped to the C-terminal region of gL (21, 23). The diagram at the bottom of Fig. Fig.11 shows the locations of the gL features mentioned above and of the signal peptide.
The interactions between gL and gH required for proper intracellular transport, processing, and cell surface expression make it difficult to investigate the functional role of one of these glycoproteins in cell fusion and viral entry independently of the other. We generated a panel of gL linker-insertion mutants to identify regions critical for gH binding and transport and regions essential for cell fusion and viral entry. One aim was to determine whether these roles of gL could be dissociated or were linked. Characterization of 12 unique gL linker-insertion mutants showed that (i) a conserved domain of gL is critical for the physical interaction of gL with gH and for the normal processing of gH, (ii) two noncontiguous regions of gL, one of which contains the highly conserved domain, are critical for the normal conformation and function of gH/gL heterodimers, and (iii) wild-type (WT) and mutant gLs can be detected on the cell surface in the absence of gH, suggesting the possibility of an independent role for uncomplexed gL. These results support and extend previous studies suggesting that gL has a larger role in membrane fusion than serving as a chaperone for gH and that specific mutations in gL can influence the function of the gH/gL heterodimer.
The cell lines used included Chinese hamster ovary (CHO-K1; ATCC) cells, CHO cells stably expressing human HVEM (20) or nectin-1 (7), Vero cells, and Vero-gL cells carrying the HSV-1 gL gene and used for the propagation and titration of the gL-negative mutant HSV-1(KOS)gL86 (20). The CHO cell line and derivatives were grown in Ham's F-12 medium supplemented with 10% fetal bovine serum, and the Vero cell line and derivatives were grown in Dulbecco minimal essential medium containing 10% fetal bovine serum.
The GPS-LS linker scanning system (New England Biolabs) was used as recommended by the manufacturer and was applied to the HSV-1 gL insert excised from pPEP101 (24) to generate random linker-insertion mutations of gL. After religation of the library of inserts into pCAGGS and transformation of bacteria, 12 unique gL linker-insertion mutants were isolated and sequenced using PrimerN (30-mer) and PrimerS (30-mer), provided by the manufacturer. After removal of the transposon, each mutant plasmid was resequenced to verify the position of each insertion.
To quantify the cell surface expression of gH and gL, several previously described anti-gH (28) and anti-gL (21) antibodies were used. One of the anti-gH MAbs (52S) binds to gH in the presence or absence of gL or other viral proteins (12, 15, 17), and the other (53S) binds to the gH/gL heterodimer but not to gH or gL expressed alone (17). Both MAbs were used at 1:10,000 dilution. The anti-gL rabbit serum was used at 1:5,000 dilution. The anti-gL MAbs were used at 1:5,000 (VIII-62-15), 1:100,000 (VIII-82-24), 1:20,000 (VIII-87-1), 1:2,000 (VIII-200-1), and 1:100,000 (VIII-820-8) dilutions. CHO cells seeded in 96-well plates were transfected with 20 ng of plasmid expressing WT gH or empty vector, a plasmid expressing WT gL or a gL mutant, and 0.13 μl of Lipofectamine 2000 diluted in Opti-MEM (Gibco). The cells were washed once with phosphate-buffered saline (PBS) 24 h after transfection, and a cell-based enzyme-linked immunosorbent assay (CELISA) was performed as described previously (19). Briefly, after incubation of the transfected cells with the antibodies, the cells were washed, fixed, and incubated with biotinylated goat anti-rabbit immunoglobulin G or biotinylated rabbit anti-mouse immunoglobulin G (Sigma), followed by streptavidin-horseradish peroxidase (GE Healthcare) and horseradish peroxidase substrate (BioFX).
CHO cells seeded in 24-well plates were transfected with 300 ng of a plasmid expressing WT HSV-1 gH (pPEP100), 300 ng of empty vector (pCAGGS) or a plasmid expressing WT gL (pPEP101) or a gL mutant, and 1.8 μl of Lipofectamine 2000 (Invitrogen) diluted in Opti-MEM (Gibco). The cells were washed with PBS 24 h after transfection and lysed with 300 μl of lysis buffer (50 mM Tris, pH 8.0, 150 mM NaCl, 1% NP-40) containing a protease inhibitor mixture (Roche Diagnostics). Fifty microliters of lysate was saved for analysis by Western blotting. The remaining 250-μl samples were mixed with 5 μl of the anti-gH MAb 52S (28) and then with protein A/G beads (Thermo Scientific) for immunoprecipitation. The lysate and recovered immunoprecipitates were fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) on 4 to 20% gels under reducing conditions, and Western blot analysis was done using rabbit serum R137 at 1:10,000 dilution. This antiserum recognizes both gH and gL (23).
CHO (effector) cells in 96-well plates were transfected with 20 ng each of plasmids expressing the T7 RNA polymerase, gD, or gH; 30 ng of plasmid expressing gB; and 20 ng of empty vector or plasmid expressing either WT (pPEP101) or mutant gL and 0.4 μl of Lipofectamine 2000. CHO-nectin-1 or CHO-HVEM (target) cells in six-well plates were transfected with 400 ng of a plasmid carrying the firefly luciferase gene under the control of the T7 promoter, 1.8 μg of empty vector (pCAGGS), and 7 μl of Lipofectamine 2000. Two hours posttransfection, the effector cells were washed once with Opti-MEM while each target cell population was washed, detached with EDTA (0.2 g/liter in PBS), and suspended in Opti-MEM. The target cell population was overlaid (3 × 104 cells/well) on the effector population. After 10 h, the cells were washed once with PBS and lysed with 50 μl/well of 1× passive lysis buffer (Promega). The expression of luciferase was quantified by adding 50 μl/well of luciferase substrate (Promega) and measuring the light output with a Wallac 1420 plate reader (Perkin-Elmer).
The complementation assay was performed essentially as described elsewhere (16). Vero cells in six-well plates were transfected with 1.0 μg of a plasmid expressing vector alone, WT gL, or one of the mutant forms of gL. At 24 h after transfection, the Vero cells were infected with HSV-1(KOS)gL86, which has the gL gene deleted but was complemented by propagation in a gL-expressing cell line. Inoculations were carried out at a multiplicity of infection of 8 in Dulbecco minimal essential medium supplemented with 5% heat-inactivated serum for 2 h at 37°C. The medium was then removed, and extracellular virus was inactivated with citrate buffer (pH 3.0) for 1 min, followed by three washes with medium. The cells were then overlaid with 3 ml of fresh medium and incubated for 24 h at 37°C. Complemented virus was harvested 24 h after infection. The culture medium was collected to sample for the titration of released infectious virus and also for the purification of released virions. The cells were lysed for the titration of cell-associated infectious virus. The culture medium containing extracellular virus was subjected to low-speed centrifugation to pellet cells and debris. After removal of a sample for titration, the supernatant was centrifuged at 48,000 × g for 1 h at 4°C over a 10% sucrose cushion. The pellet was dissolved in sample buffer and separated by SDS-PAGE on a 4 to 20% gel under reducing conditions. Western blot analysis was performed using rabbit serum R137 at 1:10,000 dilution and VP5 MAb (EastCoast Bio) at 1:5,000 dilution. The cell lysate and supernatant were titrated on the gL-complementing Vero cell line, Vero-gL, to determine the number of PFU harvested for each gL mutant.
The random-mutagenesis scheme utilized here introduced a 5-amino-acid insertion or a stop codon. Since truncation mutants of gL had previously been characterized, we focused on the insertion mutants only. Twelve unique insertion mutants were identified. The positions of the insertions are indicated by the one-letter name and position number (counting from the Met that starts translation) of the gL amino acid immediately preceding the inserted 5 amino acids (Table (Table1).1). To determine the effects of the insertions on cell surface expression of gL and gH, plasmids encoding the gL mutants were transfected into CHO cells, along with a plasmid expressing WT gH. The live cells were incubated with an anti-gL rabbit antiserum, R88, for detection of gL, and replicate cultures were incubated with the MAb 52S for detection of gH by a CELISA.
Figure Figure11 shows the level of cell surface gL detected for each mutant, as a percentage of WT gL, in relation to the amino acid position of the insertion. In addition, the level of cell surface gH detected, as a percentage of the gH levels on cells cotransfected with WT gL, is also shown. A linear representation of gL is shown below the x axis and coded to identify specific features of gL mentioned in the introduction. All gL mutants were detected on the cell surface. The two mutants with insertions after F071 and E105 exhibited the lowest levels of gL expression. Moreover, the gH coexpressed with these mutants was undetectable on cell surfaces, suggesting that they failed to associate with gH or, alternatively, associated in such a way that the 52S epitope was destroyed. Two other mutants (with insertions after R055 and T157) exhibited a reduced gH/gL detection ratio (Fig. (Fig.1),1), suggesting some aberration in the association of gL with gH. A fifth mutant (with an insertion after A185) exhibited a reduced level of gL expression, but not of gH expression. The other seven mutants were indistinguishable from WT gL in their levels of expression on the cell surface and in codetection of gH.
A previous study demonstrated that, in the absence of gH, gL was secreted by transfected cells into the medium and could not be detected on cell surfaces by immunofluorescence (2), although others (15) reported low levels of cell surface gL under these conditions. CELISAs were performed using polyclonal antibodies to detect gL on CHO cells expressing WT gL or each gL mutant in the presence or absence of gH coexpression. Figure Figure22 shows the results, expressed as percentages of the level of WT gL detected when it was cotransfected with gH. The amount of WT gL detected in the absence of gH was approximately 68% of levels in the presence of gH. For most of the gL mutants, the presence of gH increased the levels of gL detected on the cell surface, as for WT gL, consistent with the formation of stable gH/gL heterodimers that were transported to the cell surface. However, the mutants with insertions after F071 and E105 showed no increase in gL detectable on the cell surface when they were coexpressed with gH, consistent with the results in Fig. Fig.1,1, indicating that gH was not present on the cell surface. Interestingly, the mutant with an insertion after A185 exhibited significantly reduced levels on the cell surface in the absence of gH, but cell surface expression was to a large extent restored in the presence of gH, indicating that the presence of gH could compensate for its impaired association with the cell surface or for its impaired recognition by the antibodies used. Thus, our results indicate a significant level of gL detectable on CHO cell surfaces in the absence of gH (although cell surface expression was enhanced by gH coexpression for all but two mutants).
To assess the effects of the insertions on the conformation of the gH/gL complexes, CELISAs were performed on CHO cells coexpressing WT gL or each of the gL mutants and WT gH, using a MAb that recognizes the gH/gL heterodimer but not gH or gL alone (53S). The results are presented in Fig. Fig.3,3, along with parallel results obtained using MAb 52S, which recognizes gH alone. Expression of most of the gL mutants with gH resulted in approximately WT levels of gH and gH/gL on cell surfaces, as detected by 52S and 53S, respectively. Two mutants (with insertions after F071and E105) failed to promote the cell surface expression of gH, as indicated by the absence of binding of either MAb, consistent with the results shown in Fig. Fig.11 and and2.2. Another mutant (with an insertion after T157) permitted the cell surface expression of gH but with an overall decrease in detection by both the 52S and 53S MAbs, to the same relative degree. Of interest, coexpression of two mutants (with insertions after P048 and R055) with gH resulted in reduced binding by 53S compared to 52S, indicating that the gH/gL complexes formed with these gL mutants were altered in conformation compared to WT complexes.
To determine whether the mutant gLs expressed on CHO cell surfaces were grossly altered in conformation, CHO cells were transfected to express both gH and either WT gL or one of the gL mutants, and CELISAs were repeated with a panel of anti-gL MAbs (Table (Table1).1). These MAbs were from the VIII series of anti-gL MAbs that were found to map to a C-terminal region (amino acids 168 to 208) of gL (21, 23). One of these studies (23) concluded that major epitopes for these MAbs are located between amino acids 168 and 178 but did not exclude the possibility that additional sequences, downstream of 178, contributed to these epitopes (see Fig. Fig.1,1, bottom, for the locations of the epitopes). For most mutants, the ratio of MAb binding to polyclonal antibody (R88) binding was essentially equivalent to that observed for WT gL, indicating retention of at least some aspects of WT conformation (Table (Table1).1). Mutant A185, located within the C-terminal 168-to-208 domain, showed a complete loss of binding by all of the anti-gL MAbs, indicating disruption of their epitopes. This disruption probably explains the reduced binding of the rabbit anti-gL serum R88, as shown in Fig. Fig.1.1. The epitopes recognized by the MAbs may also be recognized by a significant fraction of the polyclonal antibodies in serum R88.
Three mutants (with insertions after R055, F071, and E105) showed somewhat reduced binding of several of the anti-gL MAbs relative to R88 binding. Insertions in these mutants appeared to affect the conformation of the MAb epitopes, which are clearly located downstream of the insertions. Since these insertions also impaired the association of gL with gH (Fig. (Fig.11 to to3)3) or altered an epitope dependent on gH/gL heterodimerization (Fig. (Fig.3),3), it seems likely that the conformations of the epitopes recognized by some of the anti-gL MAbs is in part dependent on proper gH/gL heterodimerization.
Coimmunoprecipitation and Western blot analyses were performed. CHO cells expressing WT gH and either WT gL or one of the gL mutants or no gL were lysed, and the cell lysates were mixed with the anti-gH MAb 52S, which recognizes gH whether or not it is complexed with gL. Figure Figure44 shows the results of the Western blot analysis for both the coimmunoprecipitates and cell lysates, using polyclonal antibodies capable of recognizing both gH and gL. For most of the mutants, polypeptides corresponding in size to gL and recognized by the gL-specific antibodies were coprecipitated with gH by the anti-gH MAb, with results similar to those observed for WT gL. Four mutants (with insertions after R055, F071, E105, and T157) exhibited reduced levels of coprecipitated gL, particularly in the case of the mutants with insertions after F071 and E105. For these four mutants, there were also reduced levels of both gH and gL in the lysates, as well as in the immunoprecipitates. In cases where the mutant gL did not associate with gH (F071 and E105), gH would likely be targeted for degradation and most of the gL could be secreted. Two of the insertions that permitted formation of gH/gL heterodimers (R055 and T157) also appeared to cause reduced stability of both gH and gL, consistent with reduced levels of gH/gL on cell surfaces (Fig. (Fig.3).3). Interestingly, the mutant with its insertion after P048 was not distinguishable from WT gL according to the coimmunoprecipitation results and Western blot analysis shown in Fig. Fig.4,4, despite effects of this insertion on gH/gL conformation as assessed by 53S binding (Fig. (Fig.33).
To assess cell fusion activity by a quantitative luciferase-based assay, WT gL or the 12 gL mutants were coexpressed with HSV-1 gB, gD, and gH in CHO cells (effectors), which were mixed with nectin-1-expressing or HVEM-expressing CHO cells (targets). CELISAs were performed in parallel to quantify cell surface expression of gL in the effector cell populations (the results were not significantly different from those shown in Fig. Fig.11 for CHO cells cotransfected with gH and gL only). Figure Figure55 presents cell surface expression for all the gL mutants in comparison with cell fusion results obtained with both the HVEM-expressing and nectin-1-expressing target cell populations. Cell fusion activity was virtually absent for three mutants (with insertions after R055, F071, and E105), was drastically impaired for two others (with insertions after P048 and T157), and was slightly impaired for two additional mutants (with insertions after L107 and F112) regardless of the fusion receptor expressed by the target cells. The mutant with an insertion after A185 did not differ significantly from WT gL in cell fusion activity, indicating that disruption of the MAb epitopes (Table (Table1)1) was without effect on this activity. The remaining four mutants were also nearly indistinguishable from WT gL in cell fusion activity.
To address whether the mutant forms of gL were functional for viral entry, the mutants were tested using a virus complementation assay. Infectious complemented virus was quantified by plaque assay both from the medium of the cells that produced it and from lysates of these cells (Fig. (Fig.6).6). Yields of complemented virus were about 1,000 times higher after complementation with WT gL than in the absence of complementation (with no gL transfected into the cells). Most of the infectious virus produced was cell associated after complementation with WT gL or any of the mutants, as is true for WT HSV-1. The three mutants that had no cell fusion activity (with insertions after R055, F071, and E105) also failed to complement the gL-null virus for cell entry. Of the two mutants that had impaired cell fusion activity, the one with its insertion after T157 had no complementation activity for viral entry, whereas the one with its insertion after P048 had about 10% of the complementation activity of WT gL.
To assess the ability of gL-complemented virus to incorporate gH into virions, we purified complemented virus from the medium of infected cells and performed Western blots to detect gH, and also the major capsid protein VP5 as a loading control (Fig. (Fig.7).7). The results showed that gH could be detected in the virions complemented with gL that retained cell fusion activity and viral entry activity. However, little if any gH could be detected in the virions produced in the absence of gL or in the presence of gL mutants that were impaired for, or devoid of, cell fusion activity (Fig. (Fig.5)5) and complementation activity (Fig. (Fig.6).6). Thus, the gL mutations that were most deleterious for cell fusion activity also impaired incorporation of gH/gL into virions. Therefore, it could not be determined whether gH/gL complexes that did form (with gL having insertions after P048, R055, or T157) would have had any activity in viral entry.
Three classes of gL insertion mutants are described in this study. The first includes mutants with insertions that had little or no effect on gL expression, association with gH, or gH/gL function. It was not surprising that insertions into the C terminus (A185 and P195) were tolerated, inasmuch as they mapped to the region that can be deleted without loss of gL function (17, 23). It was also not surprising that the insertion into the signal peptide (G005) had little effect; it did not have much effect on the predicted efficiency of signal peptidase cleavage at the WT cleavage site. On the other hand, insertions L107, F112, S135, and A140 also had little if any effect on gL expression and function despite their locations between two regions where insertions had severely negative effects on gL activities. Thus, these insertions themselves and the increase in length of the polypeptide chain between positions L107 and A140 were well tolerated, suggesting flexibility of the three-dimensional structure in this region.
The other two classes of insertion mutants include all those that abrogated or reduced gL function. In two cases (the second class), the insertions (F071 and E105) prevented any detectable interaction of gL with gH, thus accounting for the absence of cell fusion activity and the failure to complement viral infectivity. This is consistent with previous studies emphasizing that the functional unit for HSV-induced cell fusion and viral entry is the gH/gL heterodimer, in part because associations between gH and gL are necessary for the proper processing of both and for their incorporation into virions (12, 15, 17, 23, 26). It is of interest that insertions F071 and E105 map within the borders of the only region in gL that is highly conserved among alphaherpesvirus members of the gL family (Fig. (Fig.11 and and8).8). This suggests that this conserved domain is a large part of the gL interface with gH, for HSV at least and perhaps also for other alphaherpesviruses.
In three cases (the third class), the insertions (P048, R055, and T157) reduced or abrogated gL function in cell fusion and complementation of viral infectivity without preventing gL interaction with gH and cell surface expression of gH/gL heterodimers. For all three of these mutants, the insertions permitted near-normal levels of gL cell surface expression with reduced levels of gH cell surface expression for insertions R055 and T157. The gH/gL heterodimers formed appeared to be conformationally aberrant in the cases of the P048 and R055 insertions. Thus, the severe reduction in cell fusion activity of these mutants is not explained simply by the failure of gL to associate with gH, but rather by (i) alterations in other functional domains of gL, (ii) effects of the gL insertions on gH conformation, or (iii) effects of the gL insertions on the overall conformation of the gH/gL heterodimer. The possibility exists, therefore, that gL contributes to the essential function of the gH/gL heterodimer in cell fusion (and in incorporation into virions) rather than just serving as a chaperone for gH processing and transport. This idea has been proposed previously for HSV (16) and for Epstein-Barr virus (25) on the basis of amino acid substitutions that reduced activity to a larger extent than heterodimer formation or transport of gH/gL to cell surfaces. In the case of HSV, these amino acid substitutions were in the vicinity of insertion T157.
Unexpectedly, WT gL and all the gL mutants were detected on the cell surface in the presence or absence of gH, as well as being secreted in the absence of gH. These findings suggest that signal peptidase cleavage may not go to completion in CHO cells, resulting in the signal peptide serving as a membrane anchor for a fraction of the gL produced. For another herpesvirus, Epstein-Barr virus, the signal peptide of gL is largely not removed by cleavage so that the protein can be oriented in membranes as a type 2 glycoprotein or secreted (18). An alternative explanation for cell surface detection of HSV-1 gL in the absence of gH is the potential existence of a cell surface receptor for HSV-1 gL. It has been proposed that herpesvirus gLs may be related to chemokine receptor ligands (36). Further study is required to resolve this issue.
It was somewhat surprising to find that epitopes on gL recognized by MAbs capable of inhibiting cell fusion (21) could be disrupted without any detectable effects on cell fusion activity. An insertion at A185 completely abrogated binding by all the MAbs tested here (Table (Table1),1), indicating that their epitopes were disrupted by this insertion and must include at least a short region downstream of amino acids 168 to 178, the minimal region identified in a previous epitope-mapping study (23). The finding that A185 is detected at lower levels on the cell surface by the R88 polyclonal antiserum, relative to WT gL, without a corresponding decrease in the gH level (Fig. (Fig.1)1) also suggests that major epitopes for R88 antibodies lie within the same region. However, the loss of this epitope(s) for the MAbs and the antiserum did not affect cell fusion activity, despite an earlier study showing that these MAbs could block the fusion of cells infected by an HSV-1 syncytial mutant, but not virus entry, even though the MAbs bound to virions (20). Based on these findings, one would predict that a mutant virus carrying the A185 insertion would not have its cell fusion activity inhibited by these MAbs.
The apparently low levels of mutant A185 detected by the rabbit antiserum on cell surfaces in the absence of gH and the restoration of A185 mutant levels in the presence of gH (Fig. (Fig.2)2) suggests several possibilities. Perhaps, in the absence of gH, A185 adopts an altered conformation that reduces the binding of antibodies in the antiserum. The low levels of the A185 mutant detected on the cell surface could also be accounted for by aberrant transport or processing that disrupts entry into the secretory pathway in the absence of gH. A third intriguing possibility is that mutant A185 has greatly reduced ability to bind to the cell surface in the absence of gH. An insertion at position 185 could theoretically disrupt a region on gL responsible for binding of gL to a putative cell surface receptor.
In this study, we identified critical functional regions in HSV-1 gL, including a gH-binding domain and additional contiguous and noncontiguous regions that are important for gH/gL function in cell fusion and viral complementation. Since gL can be either secreted or associated with the cell surface in the absence of gH, the possibility that gL on its own may have another role in virus infection should be explored.
We thank N. Susmarski for excellent technical assistance and R. Longnecker for helpful discussions on this project and the manuscript. We also thank R. Eisenberg and G. Cohen (University of Pennsylvania) for the gift of antiserum R137.
This work was supported by Public Health Service grants CA021776 from the National Cancer Institute and AI036293 from the National Institute of Allergy and Infectious Diseases.
Published ahead of print on 2 September 2009.