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Logo of bmcgenoBioMed Centralsearchsubmit a manuscriptregisterthis articleBMC Genomics
 
BMC Genomics. 2009; 10: 478.
Published online Oct 16, 2009. doi:  10.1186/1471-2164-10-478
PMCID: PMC2770530
Maternal high fat diet during pregnancy and lactation alters hepatic expression of insulin like growth factor-2 and key microRNAs in the adult offspring
Junlong Zhang,corresponding author1 Fang Zhang,1 Xavier Didelot,2 Kimberley D Bruce,3 Felino R Cagampang,3 Manu Vatish,1,4 Mark Hanson,3 Hendrik Lehnert,1,5 Antonio Ceriello,6 and Christopher D Byrne3
1Clinical Science Research Institute, Warwick Medical School, Clinical Sciences Building, University Hospital - Walsgrave Campus, Clifford Bridge Road, Coventry, CV2 2DX, UK
2Department of Statistics, University of Warwick, Coventry, CV4 7AL, UK
3Institute of Developmental Sciences, Developmental Origins of Health and Disease Division, University of Southampton Medical School, Southampton General Hospital, Southampton, SO16 0YD, UK
4Albert Einstein College of Medicine, 1301 Morris Park Avenue, Bronx, New York, NY 10461, USA
51st Medical Department, University of Lübeck Medical School Ratzeburger Allee 160, 23538 Luebeck, Germany
6Chair of Endocrinology, University of Udine, Italy
corresponding authorCorresponding author.
Junlong Zhang: jlz203/at/yahoo.co.uk; Fang Zhang: zf501_ren/at/hotmail.com; Xavier Didelot: X.Didelot/at/warwick.ac.uk; Kimberley D Bruce: K.D.Bruce/at/soton.ac.uk; Felino R Cagampang: f.cagampang/at/soton.ac.uk; Manu Vatish: M.Vatish/at/warwick.ac.uk; Mark Hanson: m.hanson/at/soton.ac.uk; Hendrik Lehnert: H.Lehnert/at/warwick.ac.uk; Antonio Ceriello: antonio.ceriello/at/uniud.it; Christopher D Byrne: C.D.Byrne/at/soton.ac.uk
Received February 13, 2009; Accepted October 16, 2009.
Abstract
Background
miRNAs play important roles in the regulation of gene functions. Maternal dietary modifications during pregnancy and gestation have long-term effects on the offspring, but it is not known whether a maternal high fat (HF) diet during pregnancy and lactation alters expression of key miRNAs in the offspring.
Results
We studied the effects of maternal HF diet on the adult offspring by feeding mice with either a HF or a chow diet prior to conception, during pregnancy and lactation, and all offspring were weaned onto the same chow diet until adulthood. Maternal HF fed offspring had markedly increased hepatic mRNA levels of peroxisome proliferator activated receptor-alpha (ppar-alpha) and carnitine palmitoyl transferase-1a (cpt-1a) as well as insulin like growth factor-2 (Igf2). A HF diet induced up-regulation of ppar-alpha and cpt-1a expression in the wild type but not in Igf2 knock out mice. Furthermore, hepatic expression of let-7c was also reduced in maternal HF fed offspring. Among 579 miRNAs measured with microarray, ~23 miRNA levels were reduced by ~1.5-4.9-fold. Reduced expression of miR-709 (a highly expressed miRNA), miR-122, miR-192, miR-194, miR-26a, let-7a, let7b and let-7c, miR-494 and miR-483* (reduced by ~4.9 fold) was validated by qPCR. We found that methyl-CpG binding protein 2 was the common predicted target for miR-709, miR-let7s, miR-122, miR-194 and miR-26a using our own purpose-built computer program.
Conclusion
Maternal HF feeding during pregnancy and lactation induced co-ordinated and long-lasting changes in expression of Igf2, fat metabolic genes and several important miRNAs in the offspring.
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