The RNA-binding Protein KSRP Promotes the Biogenesis of a Subset of miRNAs

doi:10.1038/nature08025

Nature. Author manuscript; available in PMC 2009 December 18.

Published in final edited form as:

Nature. 2009 June 18; 459(7249): 1010–1014.

Published online 2009 May 20. doi: 10.1038/nature08025

PMCID: PMC2768332

NIHMSID: NIHMS105928

The RNA-binding Protein KSRP Promotes the Biogenesis of a Subset of miRNAs

Michele Trabucchi,¹ Paola Briata,²^* MariaFlor Garcia-Mayoral,³ Astrid D. Haase,⁴ Witold Filipowicz,⁴ Andres Ramos,^3,⁵ Roberto Gherzi,^* and Michael G. Rosenfeld^*

¹Howard Hughes Medical Institute, Department and School of Medicine, University of California, San Diego, 9500 Gilman Drive, Room 345, La Jolla, California 92093-0648, USA

²Istituto Nazionale per la Ricerca sul Cancro (IST), Largo R. Benzi, 10; 16132 Genova, Italy

³Division of Molecular Structure, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, U.K.

⁴Friedrich Miescher Institute for Biomedical Research, P.O. Box 2543, 4002 Basel, Switzerland

^*equal contributors

⁵corresponding authors ; Email: mrosenfeld/at/ucsd.edu, Email: rgherzi/at/ucsd.edu, Email: aramos/at/nimr.mrc.ac.uk.

Author Information Reprints and permissions information is available at www.nature.com/reprints. Correspondence and requests for materials should be addressed to M.G.R. (Email: mrosenfeld/at/ucsd.edu), R.G. (Email: rgherzi/at/ucsd.edu), or to AR. (Email: aramos/at/nimr.mrc.ac.uk).

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Abstract

Consistent with the role of microRNAs (miRNAs) in down-regulating gene expression by reducing translation and/or stability of target mRNAs¹, the levels of specific miRNAs are important for correct embryonic development and have been linked to several forms of cancer²^-⁴. However, the regulatory mechanisms by which primary miRNAs (pri-miRNAs) are processed first to precursor miRNAs (pre-miRNAs) and then to mature miRNAs by the multiprotein Drosha and Dicer complexes⁵^-⁸, respectively, remain largely unknown. The KH-type splicing regulatory protein (KSRP) interacts with single strand AU-rich elements (ARE)-containing mRNAs and is a key mediator of mRNA decay⁹^,¹⁰. Here, we show that KSRP also serves as a component of both Drosha and Dicer complexes and regulates the biogenesis of a subset of miRNAs. KSRP binds with high affinity to the terminal loop (TL) of the target miRNA precursors and promotes their maturation. This mechanism is required for specific changes in target mRNA expression that affects specific biological programs, including proliferation, apoptosis and differentiation. These findings reveal an unexpected mechanism that links KSRP to the machinery regulating maturation of a cohort of miRNAs, that, in addition to its role in promoting mRNA decay, independently serves to integrate specific regulatory programs of protein expression.

We analysed by mass spectroscopy the immunopurified Dicer-containing complex¹¹ and identified, amongst others, KSRP (Supplementary Fig. 1a), a highly conserved nucleo-cytoplasmic RNA-binding protein regulating distinct steps of mRNA life (¹²^,¹³ and Supplementary Fig. 1b-d). KH domains 3 and 4 of KSRP (KH3-4) are required to promote ARE-containing labile mRNAs decay⁹^,¹⁰. Coimmunoprecipitation revealed that KSRP is an integral component of the Dicer complex in HeLa cells (Fig. 1a). Upon Dicer knock-down—induced (Supplementary Fig. 2a) pre-miRNAs upregulation, an anti-KSRP antibody immunoprecipitated pre-let-7a¹⁴ (Fig. 1b). Recombinant KSRP directly interacted with pre-let-7a-1, and KH3-4 accounted for the RNA high affinity binding (Supplementary Fig. 3a). Interestingly, KSRP interacted with the TL of pre-let-7a-1 (TL-let-7a-1) while did not associate with either single- or double-stranded mature let-7a (Fig. 1c) with KH3-4 accounting for KSRP binding to TL-let-7a-1 (Supplementary Fig. 3b).

Figure 1

KSRP, a component of Dicer complex, interacts with the TL of pre-let-7a-1

We titrated the protein with increasing amounts of TL-let-7a-1 and TL-let-7a-1 with increasing amount of protein while monitoring the binding by NMR and CD, respectively. KH3-4 binds to TL-let-7a-1 with a 1:1 stoichiometry and ~50 nM K_d (Supplementary Fig. 3c) while single KH3 and KH4 domains bind to TL-let-7a-1 with ~500 nM and ~ 40 μM K_d, respectively (Supplementary Fig. 3d, e). In contrast to TNFα ARE interaction¹⁰, KH3 recognises a specific site in the TL-let-7a-1 and contributes most of the binding affinity in the KH3-4-RNA interaction, while KH4 plays an auxiliary role. Comparison of the chemical shift changes undergone by KH3 amide resonances upon RNA binding in the isolated KH3 and within the two-domain KH2-3 and KH3-4 constructs showed that the bound position of the resonance affected by TL-let-7a-1 binding is the same whether or not KH2 or KH4 are present (Fig. 1d and Supplementary Fig. 3f). NMR spectra showed that the position of nearly all of the resonances of the RNA-bound KH3 is the same for the TL-let-7a-1 and the entire pre-let-7a-1 (Supplementary Fig. 3g).

Our recent work indicated that KH3 recognises short G-rich stretches with high specificity and affinity¹⁵. The TL-let-7a-1 presents two GGG triplets, thus supporting the idea that KH3 docks KSRP on a specific site and that KSRP-prelet-7a-1 recognition takes place using a very different mode and a considerably higher affinity compared with ARE mRNA targets¹⁰^,¹⁶. Mutational analysis of TL-let-7a-1 revealed that the 5' GGG triplet accounts for high affinity binding to KSRP (Fig. 1e).

KSRP knock-down in both HeLa and NIH-3T3 cells (Supplementary Fig. 2b, c) abrogated the endogenous let-7a-mediated post-transcriptional silencing of a reporter construct containing six let-7a binding sites (let-7a6XBS) (Fig. 2a). Importantly, KSRP knock-down inhibited the effect of both transfected pri- and pre-let-7a-1 on let-7a6XBS reporter but left transfected mature let-7a function unaffected (Fig. 2b and Supplementary Fig. 4a, b). Control AUF1 knock-down (Supplementary Fig. 2d) had no effect (Fig. 2a, b).

Figure 2

KSRP regulates pre-let-7a-1 processing and controls the expression of select miRNAs

Recombinant KSRP increased the processing activity of Dicer (Fig. 2c) while KSRP immunodepletion from 293T extracts (Supplementary Fig. 2e) removed the pre-let-7a-1 processing activity (Supplementary Fig. 4c) leaving unaffected mature let-7a (Supplementary Fig. 4d). Finally, immunopurified KSRP-containing complexes specifically processed pre-let-7a-1 into mature let-7a (Supplementary Fig. 4e-g).

To investigate whether all pre-miRNAs are regulated by KSRP, we performed miRNA microarray analysis. Transient KSRP knock-down in HeLa cells significantly reduced (>1.5 fold) the expression of 14 miRNAs (Supplementary Fig. 5a) and reduced by 1.2-1.5 fold the expression of additional 20 miRNAs (Supplementary Table I). Northern blot analysis in both HeLa and NIH-3T3 cells confirmed that let-7a, miR-26b, miR-20, miR-106a, miR-21, and miR-16 were reduced by 40-70% upon KSRP knock-down while miR-23b and miR-24 were unaffected (Fig. 2d; Supplementary Fig. 5b-e, and data not shown). UV-crosslinking and in vitro processing experiments showed selectivity of KSRP binding and KSRP-induced processing for those miRNAs whose expression was regulated by KSRP (Supplementary Fig. 6a-c, and data not shown). Interestingly, the TL-miR-21 does not contain any GGG triplets but displays two potential nonoptimal binding sites for KSRP KH3 and KH4 (Supplementary Fig. 6d; ¹⁵). Mutation of the two G residues in the GUUG 5' element abrogated KSRP interaction while mutation of the 3' GG doublet only reduced the binding affinity (Supplementary Fig. 6d).

To investigate the function of TL-KSRP interaction, we utilized chimaeric pre-miRNAs with swapped loop sequences. KSRP knock-down did not affect the expression of a reporter containing let-7a binding sites when a chimaeric pre-let-7a-1 comprising the TL of miR-23b was expressed in HeLa cells (Fig. 2e). Conversely, expression of a reporter containing miR-23b binding sites was impaired by KSRP knock-down in HeLa cells expressing a chimaeric pre-miR-23b containing the TL of let-7a-1 (Fig. 2f, Supplementary Fig. 6e).

Since pre-miRNA accumulation induced by KSRP knock-down was low in comparison to that induced by Dicer knock-down (Supplementary Fig. 7a) and KSRP knock-down increased the levels of pri-let-7a-1 and pri-miR-21 (Supplementary Fig. 7b), we hypothesized an involvement of KSRP in pri-miRNA processing. KSRP coimmunoprecipitated with Flag-tagged Drosha and DGCR8 (Fig. 3a) and anti-KSRP antibody immunoprecipitated pri-let-7a-1 and pri-miR-21 but not pri-miR23b, pri-miR-24, and pri-miR-17 (Fig. 3b and Supplementary Fig. 7c). Endogenous, transfected and recombinant KSRP specifically interacted with pri-let-7a-1 (Supplementary Fig. 8a-c). Immunopurified KSRP-containing complexes processed pri-let-7a-1 similarly to Drosha-containing immunopurified complexes (Supplementary Fig. 9a). Either stable or transient KSRP knock-down in 293T, HeLa, and NIH-3T3 cells reduced the pri-let-7a-1 processing (Supplementary Fig. 9b-d) leaving unaffected the processing of pri-miR-23b (Supplementary Fig. 9b right panel and data not shown). Addition of recombinant KSRP to 293T shKSRP extracts restored pri-let-7a-1 processing while KSRP overexpression in 293T cells strongly increased pri-let-7a-1 processing (Fig. 3c and Supplementary Fig. 9e).

Figure 3

KSRP is a component of the Microprocessor complex, interacts with pri-let-7a-1 favouring its processing, and is required for both Drosha and Dicer complexes interaction with let-7a precursors

We explored the possibility that KSRP favours the association of the enzymatic complexes with select miRNA precursors. Indeed, KSRP knock-down abrogated the interaction of Drosha with pri-let-7a-1, and pri-miR-21 (Fig. 3d) and strongly reduced the binding of Dicer to pre-Let-7a-1 and pre-miR-21 (Fig. 3e). In contrast, KSRP knock-down did not affect the interaction of the same pri-miRNAs and pre-miRNAs with either DGCR8 or TRBP (Supplementary Fig. 10a and data not shown)⁵^,¹¹.

KSRP knock-down increased mRNA levels of two let-7 targets, NRAS and MYC (¹⁷^,¹⁸, Supplementary Fig. 10b), and specificity was established because cotransfection of mature let-7a abolished this effect (Supplementary Fig. 10c, d). Furthermore, KSRP knock-down in U2OS osteosarcoma cells reduced the expression of mature let-7a, significantly upregulated cell proliferation¹⁹ (Fig. 4a, and data not shown), and reduced the anti-proliferative effect of transfected pri-let-7a-1 but not of mature let-7a (Fig. 4a, Supplementary Fig. 11a). Similarly, KSRP knock-down prevented the pri-miR-16-1—induced apoptosis²⁰ while did not affect the activity of transfected mature miR-16 (Supplementary Fig. 11b, c). Recently, an essential role of select miRNAs (miR-1, miR-133a, miR-206) in C2C12 myoblasts differentiation has been reported²¹^,²². KSRP knock-down in C2C12 reduced the maturation of “myogenic” miRNAs (Supplementary Fig. 12a-c). KSRP interaction with pri-miR-206, pri-miR-1-1, and pri-miR-1-2 was increased by pro-differentiative stimuli (DM, Supplementary Fig. 12d). Finally, KSRP knock-down inhibited the miR-206-induced down-regulation of direct target mRNAs, including Connexin 43 and DNA pol α²², impairing C2C12 differentiation (Supplementary Fig. 12e, f).

Figure 4

KSRP affects let-7-regulated cell proliferation and is involved in Lin28-regulated maturation of let-7g in P19 cells

Recently, four papers⁸^,²³^-²⁵ demonstrated that the maturation of let-7 is blocked by Lin28 in undifferentiated embryonic stem cells and P19 cells. We observed that KSRP interacts with pri-Let-7g in P19 cells upon retinoic acid-induced differentiation (Fig. 4b). Lin28 knock-down in undifferentiated P19 cells induced let-7g expression while concomitant KSRP knock-down abolished this effect (Fig. 4c) suggesting that, upon Lin28 knock-down, KSRP promotes let-7g precursors maturation. Similarly, upon P19 differentiation, Lin28 expression is abrogated⁸^,²³^-²⁵ thus allowing KSRP to promote processing of let-7g precursors. This is also compatible with the recently reported mechanism of action of Lin28²⁶. We suggest that TL is a pivotal structure where miRNA processing “activators” (e.g. KSRP) as well as “repressors” (e.g. Lin28) function in a coordinated way to convey proliferating, apoptotic or differentiating cues into changes of miRNA expression (Fig. 4d).

In conclusion, KSRP is a key regulator of the processing of a sizeable subset of miRNA precursors based on its high affinity binding to their TL. The TLs of the majority of KSRP-regulated microRNAs (let-7-a, -b, -c, -d, -f, -i, and miR-196a) contain short G-rich stretches of at least 3 Gs that represent the optimal binding site for KH3¹⁵. However, the TLs of the other KSRP target miRNAs contain instead two sequential or isolated Gs and our data on KSRPTL-miR-21 interaction show that a significant, albeit different, contribution to the binding is provided by both Gs motifs (Supplementary Fig. 6d). These data underscore the adaptability of the protein to a broad range of single-strand RNA sequences¹⁵^,¹⁶.

Upon binding, KSRP could optimise the positioning/recruitment of both the miRNA precursors processing complexes through protein-protein interactions (Supplementary Fig. 13a-c). The RNase sensitivity of KSRP—exportin-5 (Exp5), interaction suggests that KSRP is associated with the terminal loop of target miRNA precursors during nucleo-cytoplasmic transit (Supplementary Fig. 13a). Sequential immunoprecipitation experiments indicate that at least two pools of miRNA precursors exist, one associated with processing complexes including KSRP and the other associated with processing complexes that do not include KSRP (Supplementary Fig. 14). For example, Caceres and coworkers²⁷ reported that hnRNPA1 binds to the TL of miRNAs whose processing is not affected by KSRP.

Altogether, our findings uncover an additional level of complexity for miRNA-dependent regulation of gene expression that contributes to the modulation of different biological programs.

METHODS SUMMARY

Ribonucleoprotein complexes immunoprecipitation (RIP)

RIP was performed as previously described by Chen et al²⁸ with minor modifications. Briefly, cells lysates were immunoprecipitated with either Protein A- or ProteinA/ProteinG-Sepharose-coupled antibodies at 4° C overnight. Pellets were sequentially washed with the following buffers: Buffer I (0.1% SDS, 1% Triton X-100, 2 mM EDTA, 20 mM Tris-HCl, pH 8.1, 150 mM NaCl); Buffer II (0.1% SDS, 1% Triton X-100, 2 mM EDTA, 20 mM Tris-HCl, pH 8.1, 500 mM NaCl); and Buffer III (0.25 M LiCl, 1% NP-40, 1% deoxycholate, 1 mM EDTA, 10 mM Tris-HCl, pH 8.1). Total RNA was prepared using Trizol®, retrotranscribed using random primers and amplified by PCR. The primer sequences are detailed in Supplementary Table II.

METHODS

Identification of Dicer-interacting proteins by liquid chromatography tandem mass spectrometry (LC-MSMS) analysis

Anti-Dicer mAbs 33, 73, and 83 and control mAbs¹¹ were crosslinked to Protein G Sepharose 4 Fast Flow (Amersham Bioscience) and used to purify Dicer complexes from HEK293T (293T) cytoplasmic extracts (S10¹¹). Coimmunoprecipitates were washed five times with lysis buffer (20 mM Tris-HCl [pH 7.5], 300 mM NaCl, 0.5% NP-40, 2.5 mM MgCl2). Proteins were separated by 10% SDS PAGE. Protein containing gel fragments, were digested with trypsin according to Schrimpf et al²⁹ and analysed by LC-MSMS (LCQ Deca XP, Thermo 7 Finnigan). Proteins were identified using Turbo Sequest and MASCOT, searching SwissProt database restricted to human proteins. A protein was considered as identified, if at least two peptides were in the first rank, concerning the correlation of experimental with theoretical data, with an ion score greater than 25. Peptides with ion scores between 20 and 40 were peer reviewed for their quality of alignment. No KSRP-specific peptides were identified in immunoprecipitations with isotype-control mAbs.

NMR and CD spectroscopy

All CD spectra were recorded on Jasco J-715 spectropolarimeter (Jasco) equipped with a PTC-348 Peltier temperature-control system. RNA binding was monitored by adding increasing amounts of protein to 2 μM TL-let-7a-1 RNA in 10 mM Tris-HCl, pH 7.4, 100 mM NaCl, 0.5 mM TCEP. A temperature of 5 °C was chosen to optimise the signal change upon protein binding. The integral of the signal between 255 and 265 nm was fitted against the protein concentration using in house programs previously described³⁰ and the values of the Kds were extracted. All NMR spectra were recorded on Bruker Avance spectrometers operating at 600 and 700 MHz 1H frequencies fitted with a supercooled probe. The spectra were processed with the NMRPipe package³¹ and analysed with Sparky³². Solutions of 25 μM ¹⁵N- labeled samples of KH3, KH4 and KH3-4 in 10 mM Tris-HCl buffer, 50 mM NaCl, 1 mM TCEP, pH 7.4, were titrated with TL-let-7a-1 RNA oligonucleotides. ¹⁵N-¹H HSQC spectra were recorded at each point of the titration at 27 °C. Amide chemical shift changes as a function of protein/RNA ratio were fitted to obtain the K_d values for the complexes using in-house software as described by Martin et al³⁰. Weighted average values of ¹⁵N and ¹H chemical shift variations have been calculated as follows: Δδ_av = [(Δδ¹H)² + (Δδ¹⁵N)²/10]^1/2 and used to map TL-let-7a-1 binding on a MolMol-generated molecular surface³³.

Preparation of RNA substrates and in vitro processing assays

For pri-miRNAs processing assays, total cell extracts were prepared in 50mM Tris-HCl [pH 8.0], 150 mM NaCl, 0.5% Triton X-100, 1X Complete®, 10% glycerol) from either HeLa or 293T cells and incubated (typically 40 μg per 25 μl reaction at 37° C for the indicated times) with in-vitro synthesised and uniformly labeled pri-miRNA (5 fmol) in processing buffer containing 100 mM KCH₃COOH, 2 mM Mg (CH₃COOH)₂, 10 mM Tris-Cl (pH 7.6), 2 mM DTT, 10 mM creatine phosphate, 1 μg of creatine phosphokinase, 1 mM ATP, 0.4 mM GTP, 0.1 mM spermine, 2 units of RNasin®. Pre-miRNA processing assays were performed as previously described by Haase et al¹¹.

miRNA profiling

HeLa cells were transiently transfected with either siRNA against KSRP or siRNA against Luciferase. Total RNA was prepared using Trizol® (Invitrogen) and enriched with RNA smaller than 40 nt using PureLink® miRNA Isolation Kit. RNA was labeled with either Cy-3 or Cy-5 using the NCode miRNA Labeling System®. A dye-swap design was employed. Labeled miRNAs were hybridized, in triplicate, to the Invitrogen NCode MultiSpecies miRNA Microarray V1®. Data were collected using the GenePix®Pro 5.0 Agilent Software (Axon Instruments). Normalization and data analysis were performed using a bootstrapping method³⁴.

Recombinant proteins and antibodies

Recombinant Dicer was purified as described by Zhang et al³⁵. MBPTRBP2 was expressed in bacteria and purified as described by Dorin et al³⁶. Production of recombinant KSRP and its deletion mutants as well as of p37AUF1 have been described previously⁹. Affinity-purified rabbit polyclonal anti-KSRP antibody, and rabbit polyclonal anti-Dicer (349) were previously described⁹^,³⁷. Mouse monoclonal anti-GST was purchased from Chemicon. Mouse monoclonal anti-Flag (M2) and anti-alpha and beta-Tubulin were from Sigma. Rabbit polyclonal anti-Drosha (07-717) was purchased from Upstate, anti-DGCR8 (N-19) goat polyclonal IgG was purchased from Santa Cruz, while anti-Lin28 goat polyclonal IgG was purchased from R&D Systems.

Plasmids

Human pre-let-7a-1, and pre-miR-23b were cloned into pSUPER-gfp-neo plasmid (Oligoengine). Chimeric constructs including either the terminal loop sequence of miR-23b in the backbone of pre-let-7a-1 (UGAGGUAGUAGGUUGUAUAGUUGUGACUUAAGAUUAAACUAUACAAUCU ACUGUCUUUC) or the terminal loop sequence of let-7a-1 in the backbone of pre-miR-23b(UGGGUUCCUGGCAUGCUGAUUUUUAGGGUCACACCC ACCACUGGGAGAUAAAUCACAUUGCCAGGGAUUACC) were generated and cloned into pSUPER-gfp-neo. pCY vector³⁸ containing part of the sequence (encompassing the mature miRNA) of either primary-let-7a-1 or primary-miR-23b were generated. Three miR-23b binding sites from a region between 360 and 385 nt of semaphorin 6D 3' UTR were cloned in pMIR-REPORT plasmid (Ambion). This sequence is a potential target of miR-23b according to TargetScan prediction program (http://www.targetscan.org). Flag-Dicer (kindly provided by Dr. M. Doyle, Friedrich Miescher Institute), and Flag-TRBP contain triple Flag tag at the N-terminus of proteins expressed from the pCIneo vector (Haase, unpublished). Flag-KSRP was previously described³⁹. Luciferase reporter gene plasmid containing 6 let-7a binding sites and a plasmid containing genomic sequence encoding primary-let-7a were kind gifs of Dr. J.G. Belasco. Flag-Drosha and Flag-DGCR8 were generously provided by Dr V. Narry Kim. Myc tagged-Exportin-5 was provided by Addgene (Addgene plasmid # 12552). Part of the genomic sequence encompassing mouse pre-miR-1-2 (pri-miR-1-2) was cloned into pcDNA3 vector (Invitrogen). Mouse pre-miR-1-2 was cloned into pSUPER-gfp-neo plasmid (Oligoengine). Luciferase reporter gene plasmid containing 4 miR-1 binding sites was kindly provided by Dr. D. Srivastava.

Cell transfection, coimmunoprecipitation, and immunoblotting

Either HeLa, U2OS, P19 or NIH-3T3 cells were transiently transfected with Lipofectamine 2000 (Invitrogen). After either 48 or 72 hrs, cells were collected, washed with PBS, resuspended in lysis buffer, and the protein concentration of cell extracts determined by the Dc Protein assay (Bio-Rad). When required, cell lysates were incubated at room temperature with either RNase A (10 μg/ml, Ambion) or RNase V1 (1 U/ml, Ambion) for 30 or 15 min, respectively. 300 μg of protein were immunoprecipitated with Protein A-Sepharose-bound anti-KSRP antibody for 16 hr at 4°C with rotation. Immunoprecipitates were washed four times with lysis buffer and resuspended in SDS protein loading buffer. Proteins were subjected to SDS-PAGE, electroblotted onto PVDF membranes, and probed with antibodies as indicated. Anti-connexin 43 antibody was from Sigma, anti-DNA pol α (G-16) was from Santa Cruz, and anti-Myogenin (F5D) was from Iowa Hybridoma Bank. C2C12 myoblasts were cultured and treated as previously described⁴⁰ and transfected using Lipofectamine 2000.

Northern blot analysis

RNA was extracted using Trizol®, resolved on 15% polyacrylamide-urea gels, and electroblotted onto HyBond N+® membranes. Membranes were hybridized overnight with radiolabeled antisense miRNAs in ExpressHyb® solution (Clontech). After hybridization, membranes were washed three times with 2X SSC and 0.05% SDS, twice with 0.1X SSC and 0.1% SDS, exposed overnight to imaging screens, and analysed using a Storm 860 PhosphorImager®. Signals were quantitated using Imagequant V1.2®. The same blot was hybridized (upon stripping in boiling 0.1% SDS) with three distinct probes, including control U6 RNA.

Luciferase assay

Either HeLa or NIH-3T3 cells (80% confluence in 12-well plates) were transfected with Luc reporters containing either 6 let-7a binding sites (pGL3-let-7a6XBS) or 3 miR-23b binding sites (pGL3-miR-23b3XBS), or empty pGL3 plasmid together with siRNAs using Lipofectamine 2000®. For some experiments cells were co-transfected with either pri-let-7a-1, or pre-let-7a-1 expression plasmids or mature let-7a. A co-transfected beta-galactosidase-containing plasmid was used to normalize firefly luciferase activity.

EdU incorporation for proliferation assay

72 hrs after transfection, cells were incubated for 2 hrs with EdU-containing medium. Nuclear incorporation of EdU was determined using Click-iT™ EdU imaging Kit (Invitrogen).

Crystal violet proliferation assay

72 hrs after transfection U-2OS cells, were fixed and stained with crystal violet solution. After two washes with water crystal violet staining was measured by spectrophotometer at λ 590 nm.

Tunel assay

Tunel-fluorescent staining of apoptotic cells was performed on U-2OS after 72 hrs from the transfection according to the manufacturer's protocol (Roche).

UV-cross linking experiments and gel mobility shift assays

UV-crosslinking experiments and gel mobility shift assays were performed essentially as described⁹^,³⁸.

Expression and purification of the recombinant proteins used for biophysical studies.

KSRP KH3, KH4 and KH3-4 proteins were obtained as previously described¹⁰. Briefly, 15N labelled proteins were expressed in Escherichia coli BL21 (DE3), as His-GST-fusion protein and initially purified using nickel affinity chromatography according to the manufacturer instructions. The His-GST fusion tags were then cleaved with TEV protease and removed using a second nickel affinity step. Proteins were further purified and buffer exchanged by gel filtration (Superdex 75 16/60 column, Pharmacia). Protein purity (always >95%) was assessed using SDS-PAGE and Coomassie blue staining. Protein quantification was achieved by a combination of spectrophotometry using predicted extinction coefficients and ninhydrin analysis of protein hydrolysates. All RNA oligonucleotides were chemically synthesized (Dharmacon).

shRNA-mediated KSRP knock-down

In order to stably knock-down KSRP the following oligonucleatides 5'-GATCAACCGGAGAGCAAGA-3' and 5'-GGACAGTTTCACGACAACG-3' for human and mouse proteins, respectively, were cloned into pSUPER-Puro (Oligoengine). Either 293T, NIH-3T3, or C2C12 cells were transfected using Lipofectamine Plus (Invitrogen). Transfectant pools were kept under selection in medium containing either 3 μg/ml (293T) or 1.2 μg/ml puromycin (NIH-3T3).

RT-PCR and Q-PCR

RT-PCR and q-PCR were performed as described in Gherzi et al⁴¹.

siRNAs

In order to knock down the following human proteins, these siRNAs were synthesized by Qiagen:

− human KSRP 5'- GAUCAACCGGAGAGCAAGAUU -3'

− mouse KSRP 5'-GGACAGUUUCACGACAACG-3'

− mouse Lin28 5'- GGGUUGUGAUGACAGGCAA-3'

− human Dicer 5'- GAAUCAGCCUCGCAACAAAUU -3'

− Luciferase 5'- CGUACGCGGAAUACUUCGAUU -3'

Supplementary Material

Click here to view.^{(6.9M, pdf)}

Acknowledgments

We are indebted to Drs. V. Narry Kim, T.C. Hobman, Ian G. Macara, D. Srivastava and J.G. Belasco for reagents, Dr. G. Corte for sharing lab facilities and critical reading, Dr. Y.T. Liu for microarray facility, Dr. M. Ponassi, and C. Nelson for technical assistance, to Drs. I. Diaz-Moreno, M. Doyle, S. Martin, and D. Hollingworth for helpful discussions and reagents, and to Drs. A. Pasquinelli and A. De Flora for helpful comments and discussions. Part of the studies have been conducted in the laboratories and facilities of the Centro Biotecnologie Avanzate (CBA, Genova, Italy). M.G.R. is an investigator with the Howard Hughes Medical Institute. M.T. is supported by a post-doctoral fellowship from the Italian Telethon Foundation. This work has been partly supported by grants from Italian I.S.S. (# 527B/2B/6), AIRC, and CIPE 2007 to R.G., I.S.S. (# 526D/39) to P.B., the EC FP6 Program Sirocco to W.F. and by NIH grants DK018477, DK39949, HL065445 to M.G.R. P.B. is recipient of a Senior Scholar Consultancy grant from A.I.C.F. Structural work on KSRP-RNA interaction is supported by grant WT022088MA from the Wellcome Trust. NMR spectra were recorded at the MRC Biomedical NMR Centre, London. The Friedrich Miescher Institute is supported by the Novartis Research Foundation.

Footnotes

Full methods and any associated references are available in the online version of the paper at www.nature.com/nature.

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