PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of jbacterPermissionsJournals.ASM.orgJournalJB ArticleJournal InfoAuthorsReviewers
 
J Bacteriol. 1967 October; 94(4): 1060–1065.
PMCID: PMC276776

Homoaconitic Acid Accumulation by a Lysine-Requiring Yeast Mutant1

Abstract

Homoaconitic acid, the second intermediate of the proposed pathway for lysine biosynthesis in yeast, is accumulated in the growth medium of a lysine-requiring mutant. This acid has been identified on paper and column chromatography by comparing it with authentic cis-homoaconitic acid. The infrared spectrum of the isolated material was identical with that of synthetic cis-homoaconitic acid. In addition, the chemical structure of the enzymatic product has been verified by degradation to glyoxylic and α-ketoglutaric acids after treatment with KMnO4 and HIO4 and by catalytic reduction to the saturated acid 1,2,4-butanetricarboxylic acid. The isolated homoaconitic acid was also identified as a substrate for a purified enzyme preparation of homoaconitase.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (811K), or click on a page image below to browse page by page.

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)