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Appl Environ Microbiol. 2009 October; 75(20): 6622–6625.
Published online 2009 August 21. doi:  10.1128/AEM.00639-09
PMCID: PMC2765155

Cloning of Salmonella enterica Serovar Enteritidis Fimbrial Protein SefA as a Surface Protein in Escherichia coli Confers the Ability To Attach to Eukaryotic Cell Lines[down-pointing small open triangle]

Abstract

The gene for the Salmonella enterica serovar Enteritidis fimbrial protein SefA was cloned into an Escherichia coli surface expression vector and confirmed by Western blot assay. E. coli clones expressing SefA attached to avian ovary granulosa cells and HEp-2 cells, providing evidence for the involvement of SefA in the ability of Salmonella to attach to eukaryotic cells.

During the 1980s to 1990s, the worldwide increase in human Salmonella enterica serovar Enteritidis infections was associated with the consumption of contaminated eggs and egg products (13, 26, 28). In the United States, grade A shell eggs were identified as a major source contributing to Salmonella infections (19, 26, 29), and the percentage of S. Enteritidis among all Salmonella isolated from outbreaks increased from 5% to 26% from 1976 to 1996 (4). Although outbreak-associated cases due to S. Enteritidis decreased from 974 during 1998 to 2000 to 692 cases in 2004 to 2006, the 28 outbreaks in 2006 still remained above the Healthy People 2010 target of 22 (6). Despite efforts directed at reducing egg-related outbreaks, S. Enteritidis infections are still among those with the highest incidence of the seven most-reported serotypes of Salmonella (5).

The large proportion of S. Enteritidis serotypes involved in food-borne outbreaks is partly attributed to the adherence elicited by surface fimbriae. Fimbriae are nonflagellar filamentous surface appendages which consist of helically arranged repeating subunit proteins called fimbrins (24). Four serologically distinct fimbriae of S. Enteritidis have been characterized according to their size (kDa) on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels: SEF21, SEF18, SEF17, and SEF14 (9-11, 21). Fimbriae can mediate the aggregation of bacteria and their attachment to inert surfaces (2) and to the surfaces of eukaryotic cells, especially to carbohydrate receptors (8, 11, 36).

SEF14 fimbriae are detected in all S. Enteritidis strains and are not widely distributed among the Enterobacteriaceae (10). These fimbriae consist of a repeating major subunit protein of 14.3 kDa (SefA) encoded by the gene sefA (9, 33). The results of studies by Peralta et al. (25) and Thiagarajan et al. (31) indicate that SEF14 fimbriae may have a role in pathogenesis by mediating attachment to eukaryotic cells. We focused on SEF14 fimbriae because of their limited distribution and their role as a main immunological target in the serological response to infection by S. Enteritidis in chickens (12). The objectives of this study were to clone and investigate the functional properties of the SEF14 fimbrin, SefA, as part of a fusion protein in Escherichia coli and to determine whether it could mediate adherence to tissue culture cells in vitro.

Bacterial strains, tissue culture cells, and plasmids used in this study are described in Table Table1.1. Bacterial cultures were grown in LB medium (Fisher Scientific, Raleigh, NC) supplemented with 0.2% glucose and shaken at 220 revolutions min−1 or in colonization factor broth (30) at 37°C. Chloramphenicol (Cm; 10 μg ml−1) and ampicillin (Amp; 50 μg ml−1) were added as needed (Sigma-Aldrich, St. Louis, MO). Avian ovary granulosa cells were grown in 5% CO2 atmosphere at 37°C in M199 medium supplemented with 26 mM NaHCO3, 0.1% bovine serum albumin, 100 U ml−1 penicillin G, and 100 μg ml−1 streptomycin sulfate (Gibco-BRL, Gaithersburg, MD). HEp-2 cells were obtained from ATCC (Rockville, MD) and grown in 5% CO2 atmosphere at 37°C in minimum essential medium (Sigma-Aldrich), pH 7.2, supplemented with 26 mM NaHCO3, 7% fetal bovine serum, 100 U ml−1 penicillin G, and 100 μg ml−1 streptomycin sulfate (Gibco-BRL and Sigma-Aldrich).

TABLE 1.
Bacterial strains, tissue culture cells, and plasmids

Primers were designed to amplify the known sefA sequence (35) corresponding to the mature protein and included restriction sites to facilitate directional cloning of the amplified target to replace the β-lactamase gene in pTX101 (27). E. coli JM109 host cells were then transformed by electroporation (Gene Pulser, Bio-Rad Laboratories, Hercules, CA). In prior studies, derivatives of pTX101 have been used successfully to express several proteins on the outer surface of E. coli cells (16, 17). The Lpp portion of pTX101 serves to localize the fusion protein to the outer membrane, while the OmpA portion traverses the outer membrane and directs the product of the cloned gene (i.e., β-lactamase or SefA) to the surface.

Membrane fractions were isolated as previously described (7) with the following modifications. Cell suspensions were sonicated with an XL series sonicator (Heat Systems, Farmingdale, NY) at 100 W and then added to ice-cold Tris-Cl, and the remaining bacteria were removed by centrifugation. The total membrane was sedimented by centrifugation (45,000 × g at 4°C) for 1 h and then suspended in Tris-Cl, and the outer membrane was isolated by adding 0.5% (wt/vol) N-lauryl sarcosine (Sigma) and shaking at 200 rpm for 30 min at 22°C to dissolve the inner membrane.

Crude SefA was obtained from S. Enteritidis CDC9 as described previously by Feutrier et al. (14). Purified SefA was obtained by the method of Chart (7) with the following modifications. Briefly, crude SefA was run on an SDS-PAGE gel and a portion corresponding to SefA was extracted from gel slices with Z-spin microcentrifuge columns (Pall/Gelman Sciences, Ann Arbor, MI). The purified SefA was vacuum dried and suspended in phosphate-buffered saline buffer.

SDS-PAGE was performed according to the method of Laemmli (18), and proteins were visualized with Coomassie blue. An identical gel was prepared for Western blots, and proteins were transferred onto BioTrace polyvinylidene difluoride (Pall/Gelman Sciences). Western blotting was done according to the membrane manufacturer's instructions (1). SefA monoclonal mouse antibodies 69/25 supplied by C. J. Thorns (Central Veterinary Laboratories, Weybridge, Surrey; 32) were used as the primary antibody; ImmunoPure goat anti-mouse immunoglobulin G(H+L) biotin-conjugated antibodies (Pierce, Rockford, IL) were used as the secondary antibody; and AVIDX-AP, assay buffer (0.1 M diethanolamine, 1.0 mM MgCl2, pH 10.0), I-Block, and CSPD (Applied Biosystems, Bedford, MA) were used in the chemiluminescent detection of the secondary antibodies using X-ray film. SDS-PAGE of total membrane fractions of E. coli JM109(pDUG3A) demonstrated a protein of the expected size for the Lpp-OmpA-SefA fusion protein (~31 kDa); however, similarly sized proteins were also observed in the control strains [JM109 and JM109(pTX101)] (Fig. (Fig.1A).1A). Western blot analysis confirmed the presence of SefA in the 31-kDa band in the recombinant strain [JM109(pDUG3A)] but not in membrane protein fractions from either the host strain alone (JM109) or the host strain harboring the pTX101 vector (Fig. (Fig.1B).1B). When outer membrane fractions were selectively isolated from the inner membrane, the presence of the 31-kDa SefA fusion protein was more readily detected over the background of proteins of similar size (Fig. (Fig.1C).1C). The data provide further evidence for the localization of the SefA fusion protein in the outer membrane of E. coli JM109(pDUG3A).

FIG. 1.
SDS-PAGE and Western blot of SefA samples using SefA monoclonal antibodies. (A) SDS-PAGE of total membrane fractions collected from cells grown at 37°C. (B) Western blot of SDS-PAGE gel shown in panel A using monoclonal mouse antibodies 69/25 ...

Attachment assays on avian ovary granulosa and HEp-2 cells were done according to the method of Thiagarajan et al. (30), with some modifications. Briefly, granulosa cells were seeded and grown as a monolayer on sterile coverslips and then washed three times with M199 medium (Gibco), and 1 × 107 CFU of bacterial cells (grown with and without 1% d-mannose) was added to the coverslips. The coverslips were then incubated in a 5% CO2 atmosphere at 37°C for 3 h, after which the cells were washed five times with M199 medium, fixed with methanol, stained with 10% Giemsa stain (Sigma), and examined by light microscopy. Attachment assays with HEp-2 cells were done as described above except that minimum essential medium (Sigma) was used as the wash solution.

Peralta et al. (25) showed that in vitro attachment of S. Enteritidis to murine intestinal epithelial cells was reduced by SefA antibodies, suggesting a role for SefA in attachment. Thiagarajan et al. (30) demonstrated attachment of S. Enteritidis to avian ovary granulosa cells that form one of the layers surrounding the yolk in a preovulatory follicle. They suggest that this attachment may be a mechanism precipitating S. Enteritidis infection of hens' ovaries that may subsequently lead to transovarian transmission to shell eggs. In our study, we compared the attachment of S. Enteritidis CDC9, E. coli JM109(pDUG3A) (sefA clone), and JM109(pTX101) (vector) to both avian granulosa (Fig. (Fig.2)2) and HEp-2 cells (Fig. (Fig.3).3). S. Enteritidis CDC9 demonstrated a mannose-resistant pattern of attachment to both granulosa and HEp-2 cells (Fig. (Fig.2A2A and and3A)3A) while JM109(pTX101) did not attach to these cell lines (Fig. (Fig.2B2B and and3B).3B). However, JM109(pDUG3A), expressing the SefA fusion protein, demonstrated mannose-sensitive attachment to both granulosa and HEp-2 cells, indicating attachment to eukaryotic cell lines attributed to the presence of SefA (Fig. (Fig.2C2C and and3C3C).

FIG. 2.
Attachment of indicated bacterial cells to avian ovary granulosa cells: S. Enteritidis CDC9 (A), E. coli JM109(pTX101) (B), and E. coli JM109(pDUG3A) (C). Cells were stained with 10% Giemsa stain and photographed with a light microscope at ×400 ...
FIG. 3.
Attachment of indicated bacterial cells to HEp-2 cells: S. Enteritidis CDC9 (A), E. coli JM109(pTX101) (B), and E. coli JM109(pDUG3A) (C). Cells were stained with 10% Giemsa stain and photographed with a light microscope at ×400 magnification. ...

Thorns (34) reported that both a wild-type S. Enteritidis strain and a sefA mutant were able to attach to HEp-2 cells, indicating that multiple determinants may mediate attachment. This is also indicated by differences in mannose sensitivities of various S. Enteritidis strains (30). The results of the Western blot and cell culture attachment assays suggest that the mannose-sensitive binding of JM109(pDUG3A) is due to the presence of SefA in the outer membrane, since JM109(pTX101) did not possess SefA or show attachment. The data also indicate that the presentation of SefA in a fimbrial structure may not be necessary for binding since nonfimbrial adhesions have been reported to mediate attachment to eukaryotic cells (22). Unfolded SefA protein in the Lpp-OmpA-SefA fusion protein may possibly allow hydrophilic or hydrophobic interactions with granulosa or HEp-2 cells, providing for mannose-sensitive adherence.

The data presented herein demonstrate that surface-expressed SefA protein may provide to nonadherent bacteria the property of adherence to eukaryotic cells and constitute further evidence for the involvement of SEF14 fimbriae in binding by S. Enteritidis. Heterologously expressed recombinant fimbrial antigens have been used successfully to induce immune responses in mice and pigs (3, 20). The results of previous studies have indicated that purified SefA protein is highly immunogenic and that SefA antibodies increase the survival rate of mice after challenge with S. Enteritidis from 32% in control mice to 78% in vaccinated mice (23, 25). The data warrant further studies to demonstrate whether the attachment observed in vitro could be demonstrated in vivo for possible applications in the competitive exclusion or induction of an immune response using this vector.

Footnotes

[down-pointing small open triangle]Published ahead of print on 21 August 2009.

REFERENCES

1. Anonymous. 1995. Blotting, hybridization and detection: an S & S laboratory manual, 6th ed. Whatman/Schleicher & Schuell Biosciences, Inc., Sanford, ME.
2. Austin, J. W., G. Sanders, W. W. Kay, and S. K. Collinson. 1998. Thin aggregative fimbriae enhance Salmonella enteritidis biofilm formation. FEMS Microbiol. Lett. 162:295-301. [PubMed]
3. Bertram, E. M., S. R. Attridge, and I. Kotlarski. 1994. Immunogenicity of the Escherichia coli fimbrial antigen K99 when expressed by Salmonella enteritidis 11RX. Vaccine 12:1372-1378. [PubMed]
4. Centers for Disease Control and Prevention. 1996. Outbreaks of Salmonella serotype enteritidis infection associated with consumption of raw shell eggs—United States, 1994-1995. MMWR Morb. Mortal. Wkly. Rep. 45:737-742. [PubMed]
5. Centers for Disease Control and Prevention. 2007. Preliminary FoodNet data on the incidence of infection with pathogens transmitted commonly through food—10 states, 2007. MMWR Morb. Mortal. Wkly. Rep. 54:352-356. [PubMed]
6. Centers for Disease Control and Prevention. 2009. Surveillance for foodborne disease outbreaks—United States, 2006. MMWR Morb. Mortal. Wkly. Rep. 58:609-615. [PubMed]
7. Chart, H. 1994. Methods in practical laboratory bacteriology. CRC Press, Boca Raton, FL.
8. Clegg, S., and G. F. Gerlach. 1987. Enterobacterial fimbriae. J. Bacteriol. 169:934-938. [PMC free article] [PubMed]
9. Clouthier, S. C., K. H. Muller, J. L. Doran, S. K. Collinson, and W. W. Kay. 1993. Characterization of three fimbrial genes, sefABC, of Salmonella enteritidis. J. Bacteriol. 175:2523-2533. [PMC free article] [PubMed]
10. Clouthier, S. C., S. K. Collinson, and W. W. Kay. 1994. Unique fimbriae-like structures encoded by sefD of the SEF14 fimbrial gene cluster of Salmonella enteritidis. Mol. Microbiol. 12:893-903. [PubMed]
11. Collinson, S. K., S. C. Clouthier, J. L. Doran, P. A. Banser, and W. W. Kay. 1996. Salmonella enteritidis agfBAC operon encoding thin, aggregative fimbriae. J. Bacteriol. 178:662-667. [PMC free article] [PubMed]
12. Cooper, G. L., and C. J. Thorns. 1996. Evaluation of SEF14 fimbrial dot blot and flagellar western blot tests as indicators of Salmonella enteritidis infection in chickens. Vet. Rec. 138:149-153. [PubMed]
13. Cox, J. M. 1995. Salmonella enteritidis: the egg and I. Aust. Vet. J. 72:108-115. [PubMed]
14. Feutrier, J., W. W. Kay, and T. J. Trust. 1986. Purification and characterization of fimbriae from Salmonella enteritidis. J. Bacteriol. 168:221-227. [PMC free article] [PubMed]
15. Francisco, J. A., C. F. Earhart, and G. Georgiou. 1992. Transport and anchoring of β-lactamase to the external surface of Escherichia coli. Proc. Natl. Acad. Sci. USA 89:2713-2717. [PubMed]
16. Francisco, J. A., C. Stathopoulos, R. A. J. Warren, D. G. Kilburn, and G. Georgiou. 1993. Specific adhesion and hydrolysis of cellulose by intact Escherichia coli expressing surface anchored cellulase or cellulose binding domains. Bio/Technology 11:491-495. [PubMed]
17. Francisco, J. A., R. Campbell, B. L. Iverson, and G. Georgiou. 1993b. Production and fluorescence-activated cell sorting of Escherichia coli expressing a functional antibody fragment on the external surface. Proc. Natl. Acad. Sci. USA 90:10444-10448. [PubMed]
18. Laemmli, U. K. 1970. Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 227:680-685. [PubMed]
19. Mishu, B., P. M. Griffin, R. V. Tauxe, D. N. Cameron, R. H. Hutcheson, and W. Schaffner. 1991. Salmonella enteritidis gastroenteritis transmitted by intact chicken eggs. Ann. Intern. Med. 115:190-194. [PubMed]
20. Morona, R., J. K. Morona, A. Considine, J. A. Hackett, L. van den Bosch, L. Beyer, and S. R. Attridge. 1994. Construction of K88- and K99-expressing clones of Salmonella typhimurium G30: immunogenicity following oral administration to pigs. Vaccine 12:513-517. [PubMed]
21. Muller, K. H., S. K. Collinson, T. J. Trust, and W. W. Kay. 1991. Type 1 fimbriae of Salmonella enteritidis. J. Bacteriol. 173:4765-4772. [PMC free article] [PubMed]
22. Ofek, I., and R. J. Doyle. 1994. Bacterial adhesion to cells and tissues. Chapman and Hall, Inc., New York, NY.
23. Ogunniyi, A. D., P. A. Manning, and I. Kotlarski. 1994. A Salmonella enteritidis 11RX pillin induces strong T-lymphocyte responses. Infect. Immun. 62:5376-5383. [PMC free article] [PubMed]
24. Paranchych, W., and L. S. Frost. 1988. The physiology and biochemistry of pili. Adv. Microb. Physiol. 29:53-114. [PubMed]
25. Peralta, R. C., H. Yokoyama, Y. Ikemori, M. Kuroki, and Y. Kodama. 1994. Passive immunization against experimental salmonellosis in mice by orally administered hen egg-yolk antibodies specific for 14-kDa fimbriae of Salmonella enteritidis. J. Med. Microbiol. 41:29-35. [PubMed]
26. Rodrigue, D. C., R. V. Tauxe, and B. Rowe. 1990. International increase in Salmonella enteritidis: a new pandemic? Epidemiol. Infect. 105:21-27. [PMC free article] [PubMed]
27. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
28. Smith, J. L., and P. M. Fratamico. 1995. Factors involved in the emergence and persistence of foodborne diseases. J. Food Prot. 58:696-708.
29. St. Louis, M. E., D. R. Morse, M. E. Potter, T. M. DeMelfi, J. J. Guzewich, R. V. Tauxe, and P. A. Blake. 1988. The emergence of grade A eggs as a major source of Salmonella enteritidis infections. New implications for the control of salmonellosis. JAMA 259:2103-2107. [PubMed]
30. Thiagarajan, D., A. M. Saeed, and E. K. Asem. 1994. Mechanism of transovarian transmission of Salmonella enteritidis in laying hens. Poultry Sci. 73:89-98. [PubMed]
31. Thiagarajan, D., A. M. Saeed, J. Turek, and E. Asem. 1996. In vitro attachment and invasion of chicken ovarian granulosa cells by Salmonella enteritidis phage type 8. Infect. Immun. 64:5015-5021. [PMC free article] [PubMed]
32. Thorns, C. J., M. G. Sojka, and D. Chasey. 1990. Detection of a novel fimbrial structure on the surface of Salmonella enteritidis by using a monoclonal antibody. J. Clin. Microbiol. 28:2409-2414. [PMC free article] [PubMed]
33. Thorns, C. J. 1995. Salmonella fimbriae: novel antigens in the detection and control of Salmonella infections. Br. Vet. J. 151:643-658. [PubMed]
34. Thorns, C. J. 1996. Studies into the role of the SEF14 fimbrial antigen in the pathogenesis of Salmonella enteritidis. Microbial. Path. 20:235-246. [PubMed]
35. Turcotte, C., and M. J. Woodward. 1993. Cloning, DNA nucleotide sequence and distribution of the gene encoding the SEF14 fimbrial antigen of Salmonella enteritidis. J. Gen. Microbiol. 139:1477-1485. [PubMed]
36. Willemsen, P. T. J., and F. K. de Graaf. 1993. Multivalent binding of K99 fimbriae to the N-glycolyl-GM3 ganglioside receptor. Infect. Immun. 61:4518-4522. [PMC free article] [PubMed]

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