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Logo of bmcgenoBioMed Centralsearchsubmit a manuscriptregisterthis articleBMC Genomics
 
BMC Genomics. 2009; 10: 456.
Published online Sep 29, 2009. doi:  10.1186/1471-2164-10-456
PMCID: PMC2763885
Transcriptomic and proteomic analyses of seasonal photoperiodism in the pea aphid
G Le Trionnaire,1 F Francis,2 S Jaubert-Possamai,1 J Bonhomme,1 E De Pauw,3 J-P Gauthier,1 E Haubruge,2 F Legeai,1 N Prunier-Leterme,1 J-C Simon,1 S Tanguy,1 and D Tagucorresponding author1
1INRA, UMR 1099 BiO3P, F-35000 Rennes, France
2Gembloux Agricultural University, Department of Functional and Evolutionary Entomology, Passage des Déportés 2, B-5030 Gembloux, Belgium
3University of Liège, Mass Spectrometry Laboratory, Sart Tilman, Liège, Belgium
corresponding authorCorresponding author.
G Le Trionnaire: glt6/at/le.ac.uk; F Francis: francis.f/at/fsagx.ac.be; S Jaubert-Possamai: stephanie.jaubert/at/rennes.inra.fr; J Bonhomme: joel.bonhomme/at/rennes.inra.fr; E De Pauw: E.DePauw/at/ulg.ac.be; J-P Gauthier: jean-pierre.gauthier/at/rennes.inra.fr; E Haubruge: haubruge.e/at/fsagx.ac.be; F Legeai: fabrice.legeai/at/rennes.inra.fr; N Prunier-Leterme: nathalie.leterme/at/rennes.inra.fr; J-C Simon: jean-christophe.simon/at/rennes.inra.fr; S Tanguy: sylvie.tanguy/at/rennes.inra.fr; D Tagu: denis.tagu/at/rennes.inra.fr
Received November 4, 2008; Accepted September 29, 2009.
Abstract
Background
Aphid adaptation to harsh winter conditions is illustrated by an alternation of their reproductive mode. Aphids detect photoperiod shortening by sensing the length of the night and switch from viviparous parthenogenesis in spring and summer, to oviparous sexual reproduction in autumn. The photoperiodic signal is transduced from the head to the reproductive tract to change the fate of the future oocytes from mitotic diploid embryogenesis to haploid formation of gametes. This process takes place in three consecutive generations due to viviparous parthenogenesis. To understand the molecular basis of the switch in the reproductive mode, transcriptomic and proteomic approaches were used to detect significantly regulated transcripts and polypeptides in the heads of the pea aphid Acyrthosiphon pisum.
Results
The transcriptomic profiles of the heads of the first generation were slightly affected by photoperiod shortening. This suggests that trans-generation signalling between the grand-mothers and the viviparous embryos they contain is not essential. By analogy, many of the genes and some of the proteins regulated in the heads of the second generation are implicated in visual functions, photoreception and cuticle structure. The modification of the cuticle could be accompanied by a down-regulation of the N-β-alanyldopamine pathway and desclerotization. In Drosophila, modification of the insulin pathway could cause a decrease of juvenile hormones in short-day reared aphids.
Conclusion
This work led to the construction of hypotheses for photoperiodic regulation of the switch of the reproductive mode in aphids.
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