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Logo of bmcgenoBioMed Centralsearchsubmit a manuscriptregisterthis articleBMC Genomics
 
BMC Genomics. 2009; 10: 460.
Published online Oct 2, 2009. doi:  10.1186/1471-2164-10-460
PMCID: PMC2761425
'Bois noir' phytoplasma induces significant reprogramming of the leaf transcriptome in the field grown grapevine
Matjaž Hren,1 Petra Nikolić,1 Ana Rotter,1 Andrej Blejec,1 Nancy Terrier,2 Maja Ravnikar,1 Marina Dermastia,corresponding author1 and Kristina Gruden1
1National Institute of Biology, Department of Biotechnology and Systems Biology, Večna pot 111, 1000 Ljubljana, Slovenia
2UMR SPO, Campus Agro-M/INRA, 2 Place Viala, 34060 Montpellier, Cedex 01, France
corresponding authorCorresponding author.
Matjaž Hren: matjaz.hren/at/nib.si; Petra Nikolić: petra.nikolic/at/nib.si; Ana Rotter: ana.rotter/at/nib.si; Andrej Blejec: andrej.blejec/at/nib.si; Nancy Terrier: terrier/at/supagro.infra.fr; Maja Ravnikar: maja.ravnikar/at/nib.si; Marina Dermastia: marina.dermastia/at/nib.si; Kristina Gruden: kristina.gruden/at/nib.si
Received March 18, 2009; Accepted October 2, 2009.
Abstract
Background
Phytoplasmas are bacteria without cell walls from the class Mollicutes. They are obligate intracellular plant pathogens which cause diseases in hundreds of economically important plants including the grapevine (Vitis vinifera). Knowledge of their biology and the mechanisms of their interactions with hosts is largely unknown because they are uncultivable and experimentally inaccessible in their hosts. We detail here the global transcriptional profiling in grapevine responses to phytoplasmas. The gene expression patterns were followed in leaf midribs of grapevine cv. 'Chardonnay' naturally infected with a phytoplasma from the stolbur group 16SrXII-A, which is associated with the grapevine yellows disease 'Bois noir'.
Results
We established an on field experimental system in a productive vineyard that allowed application of molecular tools in a plant natural environment. Global transcription profiles of infected samples were compared with the healthy ones using microarray datasets and metabolic pathway analysis software (MapMan). The two-year-long experiment revealed that plant genes involved in primary and secondary metabolic pathways were changed in response to infection and that these changes might support phytoplasma nutrition. A hypothesis that phytoplasmas interact with the plant carbohydrate metabolism was proven and some possibilities how the products of this pathway might be utilized by phytoplasmas are discussed. In addition, several photosynthetic genes were largely down-regulated in infected plants, whereas defense genes from the metabolic pathway leading to formation of flavonoids and some PR proteins were significantly induced. Few other genes involved in defense-signaling were differentially expressed in healthy and infected plants. A set of 17 selected genes from several differentially expressed pathways was additionally analyzed with quantitative real-time PCR and confirmed to be suitable for a reliable classification of infected plants and for the characterization of susceptibility features in the field conditions.
Conclusion
This study revealed some fundamental aspects of grapevine interactions with the stolbur 'Bois noir' phytoplasma in particular and some plant interactions with phytoplasmas in general. In addition, the results of the study will likely have an impact on grape improvement by yielding marker genes that can be used in new diagnostic assays for phytoplasmas or by identifying candidate genes that contribute to the improved properties of grape.
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