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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
J Neurol Sci. Author manuscript; available in PMC 2010 November 15.
Published in final edited form as:
PMCID: PMC2760614
NIHMSID: NIHMS112039

NEUROPROTECTIVE AND ANTI-INFLAMMATORY EFFECTS OF ESTROGEN RECEPTOR LIGAND TREATMENT IN MICE

Abstract

Demyelination and neurodegeneration is a major contributor in the progression of disability in multiple sclerosis (MS). Thus, the development of therapies that are neuroprotective has elicited considerable interest. Estrogens and estrogen receptor (ER) ligand treatments are promising treatments to prevent MS-induced neurodegeneration and a multicenter phase II clinical trial of estriol as beneficial therapy in MS is underway. Here, we discuss studies performed in our laboratory that examined effects of ER ligands in the inflammatory/demyelinating disorder experimental autoimmune encephalomyelitis (EAE), a model of MS. Administration of estriol or 17β-estradiol reduced clinical severity and this clinical disease improvement was associated with favorable changes in cytokine production. There was a significant decrease of neuronal pathology in gray matter along with myelin and axon preservation in white matter of spinal cords of mice with EAE. In subsequent experiments, we contrasted results of ERα versus ERβ ligand treatment. While ERα ligand treatment was anti-inflammatory, ERβ ligand treatment was not. ERβ ligand treatment nevertheless reduced demyelination and preserved axon numbers in white matter and prevented neuronal abnormalities in gray matter. Clinically, ERα ligand treatment abrogated disease at the onset, while ERβ ligand treatment had no effect at disease onset, but promoted recovery. Thus, unlike ERα ligand treatment, ERβ ligand treatment was protective at the level of the target organ, independent of anti-inflammatory effects in the peripheral immune system. ERβ ligand treatment should be considered as a potential neuroprotective agent for MS and other neurodegenerative diseases, particularly since breast and uterine cancer are mediated through ERα.

Keywords: estrogen receptor ligands, anti-inflammation, neuroprotection, EAE

1. Introduction

Recent introduction of immunomodulatory therapies have considerably improved the therapeutic options for patients with multiple sclerosis (MS). These agents reduce relapse rate, and prevent the accumulation of MRI lesion load in clinically definite MS resulting in a modest effect on delaying the progression time to disabling stages caused by neuronal loss(1, 2). There are no directly neuroprotective agents available for treatment of MS. During the course of MS the only time patients have encountered alleviation of MS symptoms, is during the late 3rd trimester of pregnancy(3). Pregnancy, by necessity, involves a relative state of immunosuppression as the fetus carries paternally derived antigens, and it is likely that high levels of estrogen associated with pregnancy contribute to this.

Endogenous ER ligands include estrone, 17-β-estradiol, and estriol; 17-β-estradiol is the predominant form of estrogen present in males and non-pregnant females while E3 is present at high levels during late pregnancy. There has been particular interest in the immunosuppressive role of estriol. Estriol is produced by the placenta that peaks during the third trimester, may be responsible for the decrease in MS-related symptoms during late pregnancy(3) (4). High dose E3 treatment has been shown to reduce the incidence and severity of EAE in two model systems (5, 6). Estriol levels appear to mirror most closely the reduction in relapse frequency seen during the third trimester of pregnancy, and there has already been a pilot study of estriol as a therapeutic agent in non-pregnant patients with MS that reported an 80% reduction in MRI disease activity over 6 months(7). A follow-on phase II/III clinical trial is currently under way of estriol and Copaxone in female MS patients.

2. Estrogen treatment improves EAE

The disease-modulating effects of estrogens have been demonstrated in CNS injury, ischemia, neurodegeneration and aging(810). Over the last 10 years, numerous studies have shown that estrogen treatment (both estriol and estradiol) administered prior to disease onset ameliorates experimental autoimmune encephalomyelitis (EAE) in mice(6, 1115). Estriol treatment when administered after disease onset is also effective in reducing EAE clinical signs(5). Finally, both estradiol and estriol are efficacious in female and male mice with EAE (16).

While a variety of anti-inflammatory mechanisms of estrogen treatment in EAE have been described, these are not mutually exclusive of more direct neuroprotective mechanisms, since estrogens are lipophilic, readily traversing the blood brain barrier(17). Some of the estrogen effects could be genomically mediated, due to interaction of ligand with the estrogen receptor isoforms α or β (ERα, ERβ). To determine whether effect of 17β estradiol and estriol mediated protection in EAE was due to stimulation of ERα alone, we treated mice with a highly selective ERα ligand, 1,3,5-tris(4-hydroxyphenyl)-4-propyl-1H-pyrazole (PPT)(18). ER specific ligands provide a pharmacological approach to study each ER implication. PPT displays 400-fold more binding affinity for ERα than ERβ and is inactive on ERβ transcriptional activity(19). Whereas 2,3-bis(4-hydroxyphenyl)-propionitrile (DPN) displays 100-fold more affinity for ERβ than ERα and has a 170-fold greater relative potency in transcriptional assays for ERβ than for ERα (20). In a subsequent publication, we then showed differential effects of ERα and ERβ ligand treatment in EAE(21).

2.1 Differential effects of ERα and ERβ ligands on clinical severity of EAE

EAE was induced in wild type, ERα or ERβ deficient mice, each treated with 17-β estradiol, the highly selective ERα (PPT) or ERβ (DPN) ligand. 17β-estradiol treatment ameliorated clinical disease in wild type (Figure 1A) and in ERβ knock(18). ERα ligand treatment ameliorated clinical disease in both wild type and ERβ knock out mice(Figure 1A, C), but not in ERα knock out mice(18, 21). In contrast, ERβ ligand treatment had no significant effect early in disease (up to day 20 after disease induction), but then demonstrated a significant protective effect later in disease (after day 20) (Figure 1B(21)). Our data showing a protective effect using the ERβ ligand DPN in active EAE in C57BL/6 mice were surprising given that another ERβ ligand (WAY-202041) was shown to have no effect in adoptive EAE in SJL mice(22). Since WAY-202041 was shown to have a 200 fold selectivity for ERβ as compared to ERα, while DPN has a 70 fold selectivity(20), it was possible that DPN was not sufficiently selective for ERβ in vivo. The in vivo selectivity of DPN during EAE was confirmed by administration of DPN to ERβ KO mice (Figure 1C(21)). DPN-treatment was no longer protective to ERβ KO mice with active EAE. These data demonstrated the in vivo selectivity of PPT for ERα and DPN selectivity for ERβ during EAE at the dose used in our studies.

Figure 1
Differential effects with ER ligands on chronic EAE

2.2 Differential effects of ERα and ERβ ligand on Autoantigen-Specific Cytokine production

Analysis of autoantigen-specific proinflammatory cytokine production during both early and later stages of EAE indicated that ERα ligand treatment significantly reduced TNFα, IFNγ, and IL6, while increasing IL5, during both early and later stages of EAE. In contrast, ERβ ligand treatment was not statistically different from vehicle treatment at either the early or later time points (18, 21). These results indicated that while ERα ligand treatment induced favorable changes in cytokine production during the autoantigen specific immune response, ERβ ligand treatment did not.

2.3 Differential effects of ERα and ERβ ligand on CNS inflammation

The onset and progression of EAE is associated with infiltration of immune cells into the CNS. Spinal cord sections from various groups were assessed for inflammation by histology (Hemotoxylin and Eosin, H&E) and immunohistochemistry (CD3+ T cells and Mac3+ macrophages)(21). On H&E staining, vehicle treated EAE mice had extensive white matter inflammation at both the early and later (Figure 2A(21)) time points as compared to normal controls. As compared to vehicle treated EAE, this inflammation was significantly reduced by treatment with the ERα ligand(18, 21). In contrast, extensive white matter inflammation was present in the ERβ ligand treated group at both the early and later time points(21). Treatment with the ERα ligand but not the ER β ligand reduced CD3+ T lymphocytes, and Mac 3+ macrophage lineage cells at both the early(21) and later (Figure 2B,C(21)) time points. Together, these data indicated that ERα but not ERβ ligand treatment reduced inflammation in the CNS of mice with EAE.

Figure 2
Even without anti-inflammatory effect ERβ ligand treatment is neuroprotective. (A–C) Treatment with an ERα ligand, not an ERβ ligand, reduced inflammation in spinal cords of mice with EAE. Representative H&E (A ...

2.4 Preservation of neurons, axons, and myelin

Extensive demyelination and axon loss occurs at the sites of inflammatory cell infiltrates in EAE mice during early and late in disease (Figure 2(18)). Quantification of number of axons and myelin density within the dorsal column and lateral funniculus of EAE spinal cords showed significant decreases (Figure 2C(18, 21)). There was a significant loss of myelin and axons in vehicle-treated late EAE mice compared to normal control mice. In contrast to vehicle treated mice with EAE, treatment with ERα and ERβ ligand significantly attenuated the loss of axons in mice with EAE (Figure 2D). We have confirmed that estrogen treatment spared GM neuronal pathology in the spinal cord of mice with EAE (18). Similarly in the presence of ERα and ERβ ligand treatment, EAE mice showed significant improvement in neuronal numbers over vehicle treated mice during early(21) and late in disease (Figure 2E(21)).

2.5 Recovery of motor performance due to ERβ ligand treatment

To assess the clinical significance of the neuroprotective effect of ERβ ligand treatment on EAE we used motor rotarod performance test (Figure 3(21)). Vehicle treated EAE mice were unable to remain on the rotarod, beginning at day 12 after disease induction. Similar to vehicle-treated mice, ERβ ligand treated mice were also unable to remain on the rotarod apparatus, beginning at day 12, but in contrast to vehicle treated mice, ERβ treated mice later during EAE had significant recovery of their ability to remain on the rotarod(Figure 3(21)). Further, this improvement in rotarod performance late during EAE with ERβ ligand treatment was no longer observed in the ERβ KO. These data demonstrated that the DPN treatment induced recovery in motor performance later in disease was mediated through ERβ.

Figure 3
ERβ ligand treatment results in recovery of motor function in EAE.

Together these data demonstrated that ERβ ligand treatment in the presence of inflammation was neuroprotective and induced functional clinical recovery in motor performance at later time points of disease during EAE.

3. Importance of specific ER ligand treatments

Estrogen treatment has been effective in numerous neurodegenerative disease models including Parkinson’s disease, spinal cord injury, cerebellar ataxia, Down’s Syndrome, epilepsy, and some models of stroke and Alzheimer’s disease(2326), and translational work using estrogen treatment for human neurodegenerative diseases has begun. Estrogens in the form of hormone replacement therapy have been associated with side effects and therefore are not recommended for use in healthy menopausal women(27). However, a more recent analysis of the WHI data revealed that estrogen had beneficial effects when therapy was started soon after menopause, but not when hormone therapy was initiated years after menopause(28). While the risk:benefit ratio in debilitating neurodegenerative diseases is clearly different than the risk:benefit ratio in healthy individuals, optimizing efficacy and minimizing toxicity, remains the goal. Hence, determining which estrogen receptor mediates the neuroprotective effect of estrogen treatment is of central importance. The only previously described neuroprotective agents for EAE are glutamate receptor blockers(2931) and Na+ channel blockers(32, 33). Glutamate and Na+ channel blocker treatments result in a modest reduction in neurologic impairment and the effect is lost after cessation of treatment(30). In the case of Na+ channel blockers, symptoms get worse and lead to death(34). Because Na+ channel and glutamate activity are needed for normal neuronal plasticity and memory(35), treatments with these blockers may be associated with significant toxicity(3436).

Our data demonstrate that while treatment with an ERβ ligand is not anti-inflammatory, it is neuroprotective. Similar neuroprotective effects of ERβ ligand has been observed in acoustic trauma, memory and depression(3739). Differential modulation of intracellular calcium, up regulation of growth factors and activation of interacting second messenger pathways by ERβ activation are most likely involved in initiating cell survival and/or prevention of cell apoptosis during disease(4043) The neuroprotective actions of ERβ ligand could be directly or indirectly on estrogen receptor containing neurons, astrocytes and oligodendrocytes. The selective neuroprotective effects of ERβ ligands are of clinical relevance since both breast and uterine endometrial cancer are mediated through ERα, not ERβ.

In a phase I pilot trial, as compared with pretreatment baseline, relapsing remitting patients treated with oral estriol (8 mg/day) demonstrated significant decreases in delayed type hypersensitivity responses to tetanus, interferon-gamma levels in peripheral blood mononuclear cells, and gadolinium enhancing lesion numbers and volumes on monthly cerebral magnetic resonance images. When estriol treatment was stopped, enhancing lesions increased to pretreatment levels. When estriol treatment was reinstituted, enhancing lesions again were significantly decreased(7, 44). It is interesting to note that estriol has a more than 5-fold preference for the activation of human ERβ over ERα, and it is a quantitatively predominant estrogen metabolite produced during pregnancy. The very high levels of estriol present during pregnancy may produce a differential activation of the ERβ signaling system in the pregnant woman and fetus for fulfilling various unique physiological functions(45). For neurodegenerative diseases with only a minimum inflammatory component, treatment with an ERβ ligand that possesses only neuroprotective properties may be sufficient. For diseases, such as MS, with a significant inflammatory component, a standard anti-inflammatory treatment could be used in combination with ERβ ligand treatment.

Understanding the mechanisms leading to cumulative neurological disability in patients with MS and further developing effective therapeutic strategies aimed at reducing disease progression is a major goal in MS research. Estrogens and estrogen receptor (ER) ligands are promising treatments to prevent neurodegeneration in the CNS(7, 46).

Acknowledgments

This work was supported by NIH and NMSS grants to RRV

Footnotes

Disclosure: None

Authors reported no conflict of interests

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References

1. Vercellino M, Plano F, Votta B, Mutani R, Giordana MT, Cavalla P. Grey matter pathology in multiple sclerosis. J Neuropathol Exp Neurol. 2005;64:1101–1107. [PubMed]
2. Peterson JW, Bo L, Mork S, Chang A, Trapp BD. Transected neurites, apoptotic neurons, and reduced inflammation in cortical multiple sclerosis lesions. Ann Neurol. 2001;50:389–400. [PubMed]
3. Confavreux C, Hutchinson M, Hours MM, Cortinovis-Tourniaire P, Moreau T. Rate of pregnancy-related relapse in multiple sclerosis. Pregnancy in Multiple Sclerosis Group. N Engl J Med. 1998;339:285–291. [PubMed]
4. Voskuhl RR. Gender issues and multiple sclerosis. Curr Neurol Neurosci Rep. 2002;2:277–286. [PubMed]
5. Kim S, Liva SM, Dalal MA, Verity MA, Voskuhl RR. Estriol ameliorates autoimmune demyelinating disease: implications for multiple sclerosis. Neurology. 1999;52:1230–1238. [PubMed]
6. Bebo BF, Jr, Fyfe-Johnson A, Adlard K, Beam AG, Vandenbark AA, Offner H. Low-dose estrogen therapy ameliorates experimental autoimmune encephalomyelitis in two different inbred mouse strains. J Immunol. 2001;166:2080–2089. [PubMed]
7. Sicotte NL, Liva SM, Klutch R, Pfeiffer P, Bouvier S, Odesa S, Wu TC, Voskuhl RR. Treatment of multiple sclerosis with the pregnancy hormone estriol. Ann Neurol. 2002;52:421–428. [PubMed]
8. Cyr M, Calon F, Morissette M, Grandbois M, Di Paolo T, Callier S. Drugs with estrogen-like potency and brain activity: potential therapeutic application for the CNS. Curr Pharm Des. 2000;6:1287–1312. [PubMed]
9. Kompoliti K. Estrogen and Parkinson’s disease. Front Biosci. 2003;8:s391–400. [PubMed]
10. Morissette M, Le Saux M, D’Astous M, Jourdain S, Al Sweidi S, Morin N, Estrada-Camarena E, Mendez P, Garcia-Segura LM, Di Paolo T. Contribution of estrogen receptors alpha and beta to the effects of estradiol in the brain. J Steroid Biochem Mol Biol. 2008;108:327–338. [PubMed]
11. Ito A, Bebo BF, Jr, Matejuk A, Zamora A, Silverman M, Fyfe-Johnson A, Offner H. Estrogen treatment down-regulates TNF-alpha production and reduces the severity of experimental autoimmune encephalomyelitis in cytokine knockout mice. J Immunol. 2001;167:542–552. [PubMed]
12. Matejuk A, Adlard K, Zamora A, Silverman M, Vandenbark AA, Offner H. 17 beta-estradiol inhibits cytokine, chemokine, and chemokine receptor mRNA expression in the central nervous system of female mice with experimental autoimmune encephalomyelitis. J Neurosci Res. 2001;65:529–542. [PubMed]
13. Polanczyk M, Zamora A, Subramanian S, Matejuk A, Hess DL, Blankenhorn EP, Teuscher C, Vandenbark AA, Offner H. The protective effect of 17beta-estradiol on experimental autoimmune encephalomyelitis is mediated through estrogen receptor-alpha. Am J Pathol. 2003;163:1599–1605. [PubMed]
14. Subramanian S, Matejuk A, Zamora A, Vandenbark AA, Offner H. Oral feeding with ethinyl estradiol suppresses and treats experimental autoimmune encephalomyelitis in SJL mice and inhibits the recruitment of inflammatory cells into the central nervous system. J Immunol. 2003;170:1548–1555. [PubMed]
15. Liu HB, Loo KK, Palaszynski K, Ashouri J, Lubahn DB, Voskuhl RR. Estrogen receptor alpha mediates estrogen’s immune protection in autoimmune disease. J Immunol. 2003;171:6936–6940. [PubMed]
16. Palaszynski KM, Loo KK, Ashouri JF, Liu HB, Voskuhl RR. Androgens are protective in experimental autoimmune encephalomyelitis: implications for multiple sclerosis. J Neuroimmunol. 2004;146:144–152. [PubMed]
17. Wise PM, Dubal DB, Wilson ME, Rau SW, Bottner M. Minireview: neuroprotective effects of estrogen-new insights into mechanisms of action. Endocrinology. 2001;142:969–973. [PubMed]
18. Morales LB, Loo KK, Liu HB, Peterson C, Tiwari-Woodruff S, Voskuhl RR. Treatment with an estrogen receptor alpha ligand is neuroprotective in experimental autoimmune encephalomyelitis. J Neurosci. 2006;26:6823–6833. [PubMed]
19. Harrington WR, Sheng S, Barnett DH, Petz LN, Katzenellenbogen JA, Katzenellenbogen BS. Activities of estrogen receptor alpha- and beta-selective ligands at diverse estrogen responsive gene sites mediating transactivation or transrepression. Mol Cell Endocrinol. 2003;206:13–22. [PubMed]
20. Meyers MJ, Sun J, Carlson KE, Marriner GA, Katzenellenbogen BS, Katzenellenbogen JA. Estrogen receptor-beta potency-selective ligands: structure-activity relationship studies of diarylpropionitriles and their acetylene and polar analogues. J Med Chem. 2001;44:4230–4251. [PubMed]
21. Tiwari-Woodruff S, Morales LB, Lee R, Voskuhl RR. Differential neuroprotective and antiinflammatory effects of estrogen receptor (ER)alpha and ERbeta ligand treatment. Proc Natl Acad Sci U S A. 2007;104:14813–14818. [PubMed]
22. Elloso MM, Phiel K, Henderson RA, Harris HA, Adelman SJ. Suppression of experimental autoimmune encephalomyelitis using estrogen receptor-selective ligands. J Endocrinol. 2005;185:243–252. [PubMed]
23. Heikkinen T, Kalesnykas G, Rissanen A, Tapiola T, Iivonen S, Wang J, Chaudhuri J, Tanila H, Miettinen R, Puolivali J. Estrogen treatment improves spatial learning in APP + PS1 mice but does not affect beta amyloid accumulation and plaque formation. Exp Neurol. 2004;187:105–117. [PubMed]
24. Leranth C, Roth RH, Elsworth JD, Naftolin F, Horvath TL, Redmond DE., Jr Estrogen is essential for maintaining nigrostriatal dopamine neurons in primates: implications for Parkinson’s disease and memory. J Neurosci. 2000;20:8604–8609. [PubMed]
25. Sierra A, Azcoitia I, Garcia-Segura L. Endogenous estrogen formation is neuroprotective in model of cerebellar ataxia. Endocrine. 2003;21:43–51. [PubMed]
26. Sribnick EA, Wingrave JM, Matzelle DD, Wilford GG, Ray SK, Banik NL. Estrogen attenuated markers of inflammation and decreased lesion volume in acute spinal cord injury in rats. J Neurosci Res. 2005;82:283–293. [PubMed]
27. Espeland MA, Rapp SR, Shumaker SA, Brunner R, Manson JE, Sherwin BB, Hsia J, Margolis KL, Hogan PE, Wallace R, et al. Conjugated equine estrogens and global cognitive function in postmenopausal women: Women’s Health Initiative Memory Study. Jama. 2004;291:2959–2968. [PubMed]
28. Resnick SM, Maki PM, Rapp SR, Espeland MA, Brunner R, Coker LH, Granek IA, Hogan P, Ockene JK, Shumaker SA. Effects of combination estrogen plus progestin hormone treatment on cognition and affect. J Clin Endocrinol Metab. 2006;91:1802–1810. [PubMed]
29. Smith T, Groom A, Zhu B, Turski L. Autoimmune encephalomyelitis ameliorated by AMPA antagonists. Nat Med. 2000;6:62–66. [PubMed]
30. Kanwar JR, Kanwar RK, Krissansen GW. Simultaneous neuroprotection and blockade of inflammation reverses autoimmune encephalomyelitis. Brain. 2004;127:1313–1331. [PubMed]
31. Basso AS, Frenkel D, Quintana FJ, Costa-Pinto FA, Petrovic-Stojkovic S, Puckett L, Monsonego A, Bar-Shir A, Engel Y, Gozin M, et al. Reversal of axonal loss and disability in a mouse model of progressive multiple sclerosis. J Clin Invest. 2008;118:1532–1543. [PMC free article] [PubMed]
32. Waxman SG. Sodium channel blockade by antibodies: a new mechanism of neurological disease? Ann Neurol. 1995;37:421–423. [PubMed]
33. Lo AC, Saab CY, Black JA, Waxman SG. Phenytoin protects spinal cord axons and preserves axonal conduction and neurological function in a model of neuroinflammation in vivo. J Neurophysiol. 2003;90:3566–3571. [PubMed]
34. Waxman SG. Mechanisms of disease: sodium channels and neuroprotection in multiple sclerosis-current status. Nat Clin Pract Neurol. 2008;4:159–169. [PubMed]
35. Kaindl AM, Ikonomidou C. Glutamate antagonists are neurotoxins for the developing brain. Neurotox Res. 2007;11:203–218. [PubMed]
36. Gardoni F, Di Luca M. New targets for pharmacological intervention in the glutamatergic synapse. Eur J Pharmacol. 2006;545:2–10. [PubMed]
37. Meltser I, Tahera Y, Simpson E, Hultcrantz M, Charitidi K, Gustafsson JA, Canlon B. Estrogen receptor beta protects against acoustic trauma in mice. J Clin Invest. 2008;118:1563–1570. [PMC free article] [PubMed]
38. Andreescu CE, Milojkovic BA, Haasdijk ED, Kramer P, De Jong FH, Krust A, De Zeeuw CI, De Jeu MT. Estradiol improves cerebellar memory formation by activating estrogen receptor beta. J Neurosci. 2007;27:10832–10839. [PubMed]
39. Walf AA, Frye CA. Administration of estrogen receptor beta-specific selective estrogen receptor modulators to the hippocampus decrease anxiety and depressive behavior of ovariectomized rats. Pharmacol Biochem Behav. 2007;86:407–414. [PubMed]
40. Brinton RD. The healthy cell bias of estrogen action: mitochondrial bioenergetics and neurological implications. Trends Neurosci. 2008;31:529–537. [PubMed]
41. Zhao L, Chen S, Ming Wang J, Brinton RD. 17beta-estradiol induces Ca2+ influx, dendritic and nuclear Ca2+ rise and subsequent cyclic AMP response element-binding protein activation in hippocampal neurons: a potential initiation mechanism for estrogen neurotrophism. Neuroscience. 2005;132:299–311. [PubMed]
42. Gerstner B, Lee J, Desilva TM, Jensen FE, Volpe JJ, Rosenberg PA. 17beta-estradiol protects against hypoxic/ischemic white matter damage in the neonatal rat brain. J Neurosci Res 2009 [PMC free article] [PubMed]
43. Marin-Husstege M, Muggironi M, Raban D, Skoff RP, Casaccia-Bonnefil P. Oligodendrocyte progenitor proliferation and maturation is differentially regulated by male and female sex steroid hormones. Dev Neurosci. 2004;26:245–254. [PubMed]
44. Soldan SS, Alvarez Retuerto AI, Sicotte NL, Voskuhl RR. Immune modulation in multiple sclerosis patients treated with the pregnancy hormone estriol. J Immunol. 2003;171:6267–6274. [PubMed]
45. Zhu BT, Han GZ, Shim JY, Wen Y, Jiang XR. Quantitative structure-activity relationship of various endogenous estrogen metabolites for human estrogen receptor alpha and beta subtypes: Insights into the structural determinants favoring a differential subtype binding. Endocrinology. 2006;147:4132–4150. [PubMed]
46. Voskuhl RR, Palaszynski K. Sex hormones in experimental autoimmune encephalomyelitis: implications for multiple sclerosis. Neuroscientist. 2001;7:258–270. [PubMed]