HNE increases the amount of endogenous nuclear Nrf2 in a time-dependent manner
A, Translocation of Nrf2 into the nucleus of HBE1 cells. B, Effects of HNE on Nrf2 in HepG2 cells. C, Changes in nuclear Nrf2 of HBE1 cells. D, Changes in nuclear Nrf2 of HepG2 cells. Both cells were treated with 10 μM HNE for the indicated time points. The nuclear and cytosolic extracts containing 30 μg of proteins were prepared and subjected to Western blot analysis. Anti- actin or lamin antibodies were used as markers for the cytosolic and nuclear extracts, respectively.