PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of nihpaAbout Author manuscriptsSubmit a manuscriptNIH Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Behav Brain Res. Author manuscript; available in PMC Oct 8, 2009.
Published in final edited form as:
PMCID: PMC2759113
NIHMSID: NIHMS28674
Selective Breeding for Infant Rat Separation-Induced Ultrasonic Vocalizations: Developmental Precursors of Passive and Active Coping Styles
Susan A. Brunelli and Myron A. Hofer
Developmental Neuroscience, New York State Psychiatric Institute and Department of Psychiatry, Columbia College of Physicians and Surgeons, Columbia University
Corresponding Author: Susan A. Brunelli, Ph.D., Developmental Neuroscience, Unit 40, New York State Psychiatric Institute, 1051 Riverside Drive, New York, NY 10032, USA, Tel: 212-543-5711; Fax: 212-543-5467, Email: sab9/at/columbia.edu
Human depression and anxiety disorders show inherited biases across generations, as do antisocial disorders characterized by aggression. Each condition is preceded in children by behavioral inhibition or aggressive behavior, respectively, and both are characterized by separation anxiety disorders. In affected families, adults and children exhibit different forms of altered autonomic nervous system regulation and hypothalamic-pituitary-adrenal activity in response to stress. Because it is difficult to determine mechanisms accounting for these associations, animal studies are useful for studying the fundamental relationships between biological and behavioral traits. Pharmacologic and behavioral studies suggest that infant rat ultrasonic vocalizations (USV) are a measure of an early anxiety-like state related to separation anxiety. However, it was not known whether or not early ultrasound emissions in infant rats are markers for genetic risk for anxiety states later in life. To address these questions, we selectively bred two lines of rats based on high and low rates of USV to isolation at postnatal (P) 10 days of age. To our knowledge, ours is the only laboratory that has ever selectively bred on the basis of an infantile trait related to anxiety. The High and Low USV lines show two distinct sets of patterns of behavior, physiology and neurochemistry from infancy through adulthood. As adults High line rats demonstrate “anxious”/“depressed” phenotypes in behavior and autonomic nervous system (ANS) regulation to standard laboratory tests. In Lows, on the other hand, behavior and autonomic regulation are consistent with an “aggressive” phenotype. The High and Low USV lines are the first genetic animal models implicating long-term associations of contrasting “coping styles” with early attachment responses. They thus present a potentially powerful model for examining gene-environment interactions in the development of life-long affective regulation.
Behavioral inhibition in childhood is a pattern of responding in which the child shows anxiety, distress or caution in response to novelty [16; 17; 78; 79]. Behavioral inhibition has been suggested to be a temperamental trait, defined as biologically-based stability of affect and behavior over the course of childhood through adolescence and into adulthood [133]. Thus, toddlers exhibiting behavioral inhibition continue to show behavioral inhibition as children, and are far more likely to manifest anxiety disorders as adolescents, perhaps as a consequence of or as a continuation of this temperamental trait [16, 79, 133]. Adults that had been categorized as inhibited in childhood exhibit greater functional MRI response in the amygdala to novel versus familiar faces, consistent with greater fear to novelty and anxiety, and suggesting that behavioral inhibition represents a lifelong trait with neurophysiological correlates [134].
In parallel with temperamental predisposition, insecure attachment behavior is also associated with inhibition and anxiety. Infants who at 4 months were categorized as insecurely attached, Anxious/Ambivalent are more likely to be inhibited and anxious in childhood [13, 83, 84, 138, 152]. Infant and child attachment classifications are associated with specific parenting styles, signifying that even the earliest manifestations of individual differences in behavior must also be considered in the context of parent-child interactions [96, 148]. Regardless of origin, pathologically increased separation anxiety in children has been associated with earlier onset of adult anxiety/depressive disorders [13, 128, 134, 152, 154, 155].
A significant percentage of children of mothers diagnosed with anxiety disorders exhibit insecure, Anxious/Ambivalent attachment, behavioral inhibition, or anxiety disorders [41, 95]. Likewise children of parents with panic or major depressive disorder exhibit higher rates of behavioral inhibition [16, 17] and are at greater risk for childhood anxiety and depressive disorders [166 - 169]. Separation anxiety is also seen more frequently in children from families with cross-generational depressive and anxiety disorders [152, 167]. While indicating that early separation anxiety is a marker for early affective dysregulation associated with temperament, these studies also suggest that there exists a relationship between inherited characteristics of the child, and parenting style in families either predisposed to or actively suffering from anxiety and depressive disorders (“gene-environment correlations”) [35, 69, 130, 148]. Moreover, human gene-environment correlations invariably include a wider environment consisting of psychosocial family characteristics, and socio-demographic variables [37, 130].
Behaviorally inhibited children show significant differences in heart rates in a variety of situations and across ages [97,151]. Fetuses of highly anxious women show significant heart rate increases during a maternal stressor, whereas the fetuses of non-anxious women exhibit minor heart rate decreases [102-104]. Infant risk for depression and anxiety are associated with higher heart rates and lower heart rate variability in response to stress [177, 178]. The interaction between attachment and temperament is also seen in cardiac physiology: infants assessed in the first year of life for behavioral inhibition were at 14 and 24 months highly likely to be classified as Anxious/Ambivalent, and exhibited lower heart rate variability that could be associated with high sympathetic tone or to low vagal tone. [32, 151]. However, hyperarousal, attributable to greater sympathetic activation appears to be a characteristic of anxious individuals, and particularly in children and adolescents [16, 67, 78, 79, 178, 179]. Consistent with this hyper-aroused phenotype, the corticotrophin releasing hormone gene was recently linked with childhood behavioral inhibition [132, 146, 147], suggesting up-regulation of the hypothalamic-pituitary-adrenal axis (HPA), responsiveness to stress. Thus, an inherited predisposition for early anxious temperament and Anxious/Ambivalent attachment appear to interact to produce a constellation of characteristics involving behavioral and physiological components predictive of later childhood and adult anxiety and depression disorders [57, 167].
At the other end of the spectrum of childhood disorders, aggression is a trait-like phenomenon that runs in families as well, with ample evidence for similar interactions between environmental and genetic influences [31, 35, 53, 69, 75, 130]. In keeping with the notion of temperamental continuity, childhood and juvenile aggressive psychopathologies are also associated with high levels of violence in adulthood [53]. Moreover, a number of studies have shown associations between childhood aggression and attention-deficit-hyperactivity symptoms, and suggest that the two characteristics combined are more likely to produce lifelong aggressive disorders than either one alone [18, 34, 36]
As with inhibited temperament, insecure, Avoidant attachment behavior is associated with childhood psychopathology. Children who at early ages (12 -24 months) are categorized as showing Avoidant or Avoidant/Disorganized attachment are also more likely to manifest an uninhibited temperament, more aggressive and disruptive behaviors in later childhood, and higher-than-normal activity [13, 31]. These children appear to be more vulnerable as well to psychosocial environments that increase the risk for child conduct disorder and adult antisocial behavior [34, 69].
Finally, young children showing uninhibited temperament and Avoidant attachment exhibit higher aggression scores and higher respiratory sinus arryhythmia, indicative of reduced parasympathetic control of heart rate [4, 31]. Similar relationships have been shown between autonomic activity and a history of aggression in older children [4, 31, 135]. In studies of 7-11-year-old boys and in aggressive adolescents, heart rate variability was found to be inversely related to hostility and aggression, consistent with reduced parasympathetic (vagal) antagonism to increases in heart rate [121, 122]. It has long been known that the same relationship exists between behavior and autonomic control of heart rate and of blood pressure in hostile/aggressive adults (so-called “Type A” personalities): this mode of reduced vagal control of heart rate and blood pressure is a significant predictor of cardiovascular disease [141, 142]. In summary, developmental studies have established that early aggression and reduced parasympathetic autonomic control of heart rate are markers for adult antisocial behavior and cardiovascular disease.
Although a great deal of correlational evidence points to inherited links between pathological affective regulation and physiological functioning, the conclusions drawn can only be inferential because human studies cannot examine underlying neurobiological mechanisms accounting for these associations. Animal studies, on the other hand, afford an opportunity to study the origins of these fundamental relationships. Even more interesting from a developmental perspective is the possibility of uncovering genetic processes that underlie predispositions for constellations of affective regulation, cognition, behavior, and biological processes (so-called “endophenotypes” [66]) that we call “temperament” across the lifespan. The selective breeding study to be described is one attempt to examine these relationships.
As with other infant mammals, infant rodents (pups) cry initially when separated from mothers (dams) and littermates; these cries peak at 45 kHz and are therefore called ultrasonic vocalizations or USV. Rat and mouse pups, like other mammalian species, also experience maternal separation as a stressful event, producing increased autonomic nervous system activity and cardiovascular changes, activation of the hypothalamic-pituitary-adrenal (HPA) axis, and a ramping up of noradrenergic and opioid system activity [21, 72, 73, 76]. Thus, in response to the threat posed by separation, USV appears to be a behavioral component of an overall coordinated defensive system in rodent pups to [76, 77].
Over several decades, infant rat pup USV responses to maternal separation have been well characterized pharmacologically, and studies are generally in agreement that these ultrasonic cries represent an anxiety-like state [25, 77]. The question has always been whether USV can be used as a model system for infant separation responses, as described by Bowlby [22] and others. Notwithstanding caveats addressed to whether or not rats experience so complex a construct as attachment, the behavioral and physiological profiles evidenced by infant rodents when separated from dams and littermates show a great deal of face and construct validity with other mammalian/human separation responses.
Our interest in USV as a marker for early affective regulation of attachment prompted us to embark on a selective breeding project that would test the hypothesis that rate of infant USV in response to separation was heritable [24]. Behavior-genetic studies in mice had established that the propensity for high or low rates of USV is a heritable trait based on significant dominance and additive components, as well as interactions between genes [70, 129]. Since no previous studies had addressed whether the behavior was itself a marker for more widespread and permanent predispositions, a second goal of this project was to determine whether rate of infant USV in response to separation was a component of a heritable temperamental trait expressed throughout life (24).
We therefore selectively bred for two lines of rats (High and Low USV lines) for extreme rates of USV in response to maternal separation in infancy. Figure 1 shows rates of USV in P10 pups for 2 minutes over 20 generations. As shown, rates of USV in High and Low USV line pups diverged dramatically from the randomly-bred control line (Random USV line) and each other, indicative of major gene effects [25, 29]. With respect to the second goal, the High and Low USV lines have shown widespread and distinctly different changes in systems mediating affective regulation from infancy to adulthood consistent with both early temperament and attachment styles [24].
Figure 1
Figure 1
Mean (±SEM) number of USV in P10 pups for 2 minutes (y-axis) over 20 generations (x-axis). Low and High USV lines diverged significantly in rates of USV from the Randomly-bred control line in the S1 and S3 generations respectively. Data based (more ...)
An unexpected effect of selection was that Low line birth weights have been significantly lower than both High and Random line weights since the 14th (S4) generation of breeding (Figure 2A). Line differences in mean litter weights at birth have remained stable and were still highly significantly different through Generation 28 (Figure 2B). However, by weaning Low line weights are not different from the High and Random lines for either sex, into adulthood [24]. The genetic and/or prenatal mechanisms underlying this long-term reduction in Low line birth weight are as yet unknown. Low line fetuses may be genetically programmed for smaller size, or the Low line maternal uterine environment may be somehow unfavorable for growth and development of Low line fetuses. That this may result in postnatal functional changes is suggested by data in which Low line juveniles show deficits in DA-mediated behaviors (see below) that parallel deficits in animals subjected to prenatal malnutrition resulting in low birth weights [6, 80, 112, 171-173].
Figure 2
Figure 2
A. Mean (±SEM) birth weights (grams) High, Low and Random lines, across the 14th through 21st generations: Line F(2,1196) = 44.634, p = 0.000; Generation: F(6, 1196) = 6.277, p = 0.000; Generation *Line: (12,1196) = 2.146, p = 0.012). Data based (more ...)
One conclusion arising from these data is that some of the effects of selection for low or high infant USV may be epigenetically mediated [e.g., 120], perhaps via perinatal maternal factors [58]. In the 13th generation (S13), we examined the possibility of postnatal maternal effects in the generational transmission of isolation-induced USV and behavior at P10 days. Pups in the S13 generation were cross-fostered within 48 hrs of birth between dams of the two lines (Low-pups to High-dams and High-pups to Low-dams) [30]. To control for fostering effects, other groups of pups were fostered to dams within their own lines (in-fostered). Additional (population) control data were obtained from the entire 13th generation of the selectively bred lines. P10 USV rates of cross-fostered pups in each line were almost exactly the same as rates of in-fostered pups of the same line, indicating no postnatal maternal effects on the High and Low infant USV phenotypes. However, High USV line pups cross-fostered to Low USV line dams weighed significantly less than High line in-fostered pups at P10, consistent with the idea that pre- and/or postnatal maternal-pup factors were influencing postnatal weight in the Low line. Thus, although the results provide no evidence for a postnatal maternal contribution to P10 USV phenotype, as suggested by the weight data, this limited study does not rule out either pre- or postnatal or perinatal effects on other measures of function, either in the short- or long-term.
A number of neurotransmitters have been implicated in the modulation of infant rat isolation-induced USV and in adult rat anxiety-related behaviors. Among these, influences of norepinephrine (NE), dopamine (DA) and serotonin (5-HT) systems have been most systematically observed in rat pups [25, 76]. Because High and Low line rat pups show such extraordinary extremes of USV to isolation at P10, changes in at least one of these systems seemed likely. Therefore, we examined line differences in levels and indices of activity in the three monoamine systems [26].
We found widespread changes in DA and 5-HT metabolism throughout cortico- and meso-limbic structures in both lines (e.g., Figures 34). These differences were not state-dependent, in that levels of un-manipulated pups were not different from pups exposed to 2 minutes of isolation, suggesting permanent regulatory changes in DA and 5-HT systems, in parallel with or perhaps underlying high and low infant USV rates. No changes were found in NE in any brain structures.
Figure 3
Figure 3
A. Mean (±SEM) DA and DOPAC levels in accessory cingulate cortex (ACC), measured as picograms per milligram of tissue (n=12 pups All ps < .01 per line). Low line DOPAC significantly greater than High and Random lines). From Brunelli & (more ...)
Figure 4
Figure 4
A. Mean (±SEM) levels of 5-HT (picograms per milligram of tissue) and 5-HT turnover measured as levels of 5-HIAA (picograms per milligram of tissue) in the bed nucleus of the stria terminalis (BNST) All ps < .01. High and Low line levels (more ...)
As shown in Figure 3A, in the anterior cingulate cortex, compared to Random line pups, in Low line levels of the DA metabolite DOPAC were significantly higher, indicative of significantly greater DA metabolism; this difference is also depicted as a higher DOPAC/DA ratio (Figure 3B). The cingulate cortex has been shown to play a central role in the production of distress vocalizations [94]; to integrate afferent and efferent sensory information [e.g. 176], and to regulate the intensity of affective expression to pain in humans and other mammals [88]. In the periaqueductal gray (PAG) levels of homovanillic acid (HVA), another DA metabolite, were significantly higher in Low and High than in Random line pups, again suggesting higher rates of DA metabolism in this structure as well (Figure 3C). The PAG is involved in the integration of affective and sensory information flowing from frontal and medial structures, and its columnar structures are dedicated to the expression of integrated, modality-specific functions, including cardiovascular, respiratory, motor and vocal responses [91, 165, 180].
Figure 4A shows levels of serotonin (5-HT) and its metabolite, 5-HIAA in the bed nucleus of the stria terminalis (BNST) in the three lines. In this structure both High and Low lines showed higher levels of both 5-HT and 5-HIAA, suggesting higher levels of production and neurotransmission of 5-HT. The BNST is involved in modulating anxiety to both learned and unlearned stimuli, and in cortocotropin-releasing hormone signaling in stress-induced cardiac activation [44, 109]. In the striatum (globus palladus and putamen) levels of 5-HIAA were higher in High line pups (Figure 4B), signifying greater metabolic activity in this structure involved in the coordination of species-typical motor activity [118].
The significance of these global changes in monoamine function early in life is unclear without additional information from other methods, although they clearly point toward alterations in DA- and 5-HT systems in structures that are not occurring in Random line pups. Nor is it clear that levels of activity of these monoamines have the same significance in the early postnatal period as in adulthood, since at this time both systems are undergoing rapid development in cortico- and mesolimbic structures [43, 47, 62, 63, 120, 156, 181, and see below]. Unlike adulthood, in which 5-HT generally functions as an inhibitory modulator, at this stage of life 5-HT increases neuronal activity in its role as a neurodevelopmental factor in cell growth and differentiation [11, 12, 93, 119, 182]. Similarly, the number and firing rates of spontaneously active DA neurons change during maturation of frontal and pre-limbic systems from 2 to 4 weeks of age [39, 101, 91]. Moreover, a variety of perinatal experiences produce discontinuities in the development and activity of these systems so as to permanently alter monoamine functioning in target areas [7, 38, 38]. Thus, it seems likely that the extensive changes in DA and 5-HT systems in High, and especially in Low line pups are predictive of functional alterations later in life.
Play is one of the earliest social behaviors that preweanling rats engage in that are not oriented toward the dam. In rats, play begins at about P18, shortly after eye opening and with independent feeding, and peaks during the periadolescent period between 30 and 40 days of age [98, 113, 157, 162]. As in other mammals, play in rats is thought to have paralleled the evolution of the prefrontal cortex [46, 51,117]. Consistent with this view, rat play is comprised of complex sets of behavioral interactions that have been implicated in shaping the development of social and cognitive skills in adulthood characterized by cognitive flexibility, such as turn-taking or the ability to switch cognitive strategies [48 - 50, 117].
A variety of perinatal conditions affect play behaviors in juvenile rats. Adult rats that have suffered perinatal malnutrition, or have been reared without access to play partners show similar patterns of social reactivity and inability to communicate socially [171-173]; and malnourished animals are more aggressive as adults than normally nourished controls [92, 158, 172]. Social play in adult and juvenile rats also varies by rat strain, indicating underlying genetic variation in levels of playfulness [139, 140, 158].
Since selection for an early, social, affective-related behavior such as USV may also have influenced the development of social behavior, in a recent study [28] we investigated whether selective breeding for high and low USV rates affected common indices of juvenile play behavior, and ultrasonic vocalizations associated with play (50 kHz calls) [81]. High, Low and Random line juveniles were isolated overnight and allowed to play briefly on each of three subsequent days, in same-line, same-sex sibling pairs. Interactions were observed over 10 minutes and frequencies of play behaviors, including nape contacts, pins and 50 kHz USVs were counted by pair [5, 6, 28]. Definitions of these behaviors are given in detail in [28].
Only play behaviors were reduced in the selected lines, ruling out global deficits in behavior, locomotor abilities or differences in arousal. High line juveniles had higher latencies to pin and lower levels of pinning (Figure 5A) and 50 kHz USVs (Figure 5B), but only in the first of the three play sessions (Play Session 1). Since 50 kHz USV is a reliable marker of positive affect in rats [81, 82], we interpreted the depression of pinning and USVs in High line on the first day to signify a negative affective state associated with initial conditions of exposure to a play partner. In contrast, nape contacts in High play pairs were uniformly low across all three play sessions (Figure 5C), suggesting ongoing reactivity to social isolation between play bouts, or that this specific behavior deficit may be an enduring characteristic of the High line, or both. Thus, it seemed reasonable to conclude that play deficits in High line juveniles were a function of behavioral inhibition or anxiety, consistent with their infant phenotype [25]. The results are also consistent with studies of 5-HT or GABA influences on regulation of affective states affecting social behavior [65, 118, 154].
Figure 5
Figure 5
A. Mean (±SEM)number of pins in 10 minutes of play during three successive play sessions in High, Low and Random juvenile sibling pairs. Low line significantly less than Random line. Male and female pairs are shown separately for each line. Frequencies (more ...)
In contrast, Low line juveniles were deficient in all play behaviors: both in terms of latencies to engage in play, and in frequencies of nape contact, pinning and rates of 50 kHz USV. Hypotheses for Low line play deficits can be posited: for example that Low line juveniles lack motivation to initiate and engage in play and therefore are affectively compromised. Alternatively, Low line juveniles may be motivated, but unable to “read” social signals intrinsic to play, with the result that juvenile play behavior is significantly reduced, without affecting pro-social behaviors like social investigation [54].
Similar patterns are evident in juveniles subjected to social isolation, and in juveniles lacking prefrontal cortex or striatum [50, 114, 118]. Global deficits in social play such as these are thought to result from reductions in dopamine (DA) levels and functioning [10, 54, 157]. Stimulation of DA activity increases play, probably through the combined action of pre- and postsynaptic dopamine D2 receptors [162]. Various forms of perinatal insults are known to affect DA functioning [15, 64,105-107, 110, 153, 154]. These conditions produce regionally specific effects on DA levels and DA activity (measured as DA metabolite turnover) in prefrontal cortex, hippocampus, hypothalamus and striatum [e.g., 68, 80, 85]. Thus, one likely mechanism for deficits in Low line play is via dysfunction in structures in systems mediating play. At another level, deficits in play may be associated with putative deficits in DA system function, with or without links to selected genes affecting USV.
Measurement of monoamine metabolism in juvenile brains after play are currently underway to address the question of developmental continuity in DA and 5-HT activity and function from infancy, and to correlate monoamine levels with differences in play between the lines. Future studies using more sensitive, fine-grained behavioral analyses will also aid in resolving questions about the nature of behavioral deficits affecting patterns of play in both lines, in conjunction with pharmacological interventions specifically targeting the DA systems.
If separation-induced USV rates are an indicator of an infant anxiety-like state, does it follow that extremes of infant USV rates predict anxiety-like behavior in adulthood? Human studies examining samples of children expressing childhood inhibition and adult anxiety/depression disorders suggest that this may be the case. On the other hand, expressions of infantile states are not necessarily predictive of lifelong behavioral traits. Infantile isolation-induced USV may, for example, be an instance of a class of purely infantile behaviors or “ontogenetic adaptations” specific to the ecological niche occupied by infants in the postnatal period [170].
To test this question of continuity, High, Low and Random line adults of both sexes were examined in a variety of standardized laboratory tests measuring anxiety and depression in rodents [184]. In a variation of the open field, to test fear of open spaces, High and Low line males and females were placed in a small cylinder in the open field, and the latencies at which they emerged from the cylinder were recorded. As shown in Figure 6, latencies for High line males were significantly higher than for Low line males. Similar results were seen in females (data not shown). High line adults of both sexes also traversed fewer inner squares than Lows, another indication of higher anxiety-like state.
Figure 6
Figure 6
Mean (±SEM) latency to emerge from a cylinder into an open field by males High and Low USV lines. From Zimmerberg, et al., 2005.
Figures 7A and B show two replications across generations of line differences in High versus Low adult males in the lengths of time spent floating, one of several behaviors measured in the Porsolt Swim. The Porsolt Swim is considered a reasonable model for depression-like behavior in rodents, since behavioral measures such as floating respond primarily to antidepressant, but not anxiolytic action [65]. In both generations High line animals of both sexes (female data not shown) spent more time floating than Lows, indicating a particularly reliable difference between the lines [137, 184]. Interestingly, differences between the lines in the Porsolt Swim are more robust than some anxiety measures like the plus maze, suggesting that the High infant USV phenotype may be more predictive of adult depression-like states than anxiety [24]. In this respect, the expression of High line infant and adult phenotypes resemble human family-genetic studies, in that child anxiety phenotypes tend to resolve into depressive disorders in adulthood [168].
Figure 7
Figure 7
A. (left)Mean (±SEM) duration immobile (floating) in the Porsolt Swim by males in the High, Low and Random lines in the 15th generation (S15); and B. (right) in High and Low line males in the 19th (S19) generation of selection. From Shair et al., (more ...)
In the same study, we measured levels of the neurosteroid, allopregnanolone (3-hydroxy-5-pregnan-20-one; 3α,5α-THP), a reduced metabolite of progesterone. Allopregnanolone, a positive modulator of the GABAA receptor, reduces anxiety behavior in adult rats in a variety of paradigms, including rat pup isolation calls [20, 23, 33, 59-61, 127, 164, 183]. Moreover, allopregnanolone modulates and is itself modulated by the 5-HT system and has been shown to alter depressive states via 5-HT mechanisms [68, 161]. Because neurosteroids appear to be intimately involved in stress responses [8, 9], we thought it possible that the adult behavioral differences observed between the Low and High USV lines might be due to selected differences in allopregnanolone-modulated receptor systems.
Allopregnanolone levels were measured in hippocampus and amygdala tissue in the same animals tested for behavior, and were significantly higher in Low line male (Figure 8A) and female (Figure 8B) rats than High line rats, consistent with its acute anti-depressant and anti-anxiety effects in rodents [19, 20, 23]. In both selected lines allopregnanolone levels fluctuated with the estrus cycle in females, so that higher allopregnanolone levels and reduced anxiety occurred during estrus when estrogen levels are highest; these were reversed during diestrus when estrogen levels are low (Figure 8B). It is notable that comparable changes in allopregnanolone levels have been implicated in Pre-Menstrual Syndrome disorder in women, and mechanisms underlying the relationships between fluctuations of mood with changes in ovarian hormone levels have been shown to be mediated by effects in the brain of allopregnanolone withdrawal on GABAA subunit conformation in rodent models [145, 155]. Higher allopregnanolone levels in Low line adults in both sexes suggests the ability to mount greater synthesis of allopregnanolone in brain areas mediating stress in the Low line. Alternatively, Lows may have higher baseline endogenous allopregnanolone or of its precursor, progesterone, and thus greater reserves to draw from during stress. Either process would promote enhanced functioning during stress.
Figure 8
Figure 8
Mean (±SEM) concentrations in ng/g tissue of allopregnanolone (3-,5-THP) in amygdala/hippocampal tissue of males (A), and proestrus females and diestrus females (B) from selectively bred High and Low USV lines (significant main effects of line (more ...)
Selection for infant USV has also produced distinct modes of cardiac responsiveness to stress. In a study of 18-day-old juveniles we found that compared to the Random line both the High and Low USV lines exhibit enhanced cardiac reactivity to stress [27]. Figure 9 shows heart rate changes at Baseline (Home cage), Isolation (Novel cage) and Recovery (Home cage), in 2-min epochs, in P18 juveniles that were taken from the home cage and placed individually into a novel environment, then returned to the home cage. Though neither line showed heart rate differences at baseline from Random, High line juvenile heart rates were significantly higher than Random line heart rates during isolation in the novel environment, consistent with their anxiety phenotype in infancy and in adulthood. In contrast to their apparent lack of anxiety in behavioral tests, however, juvenile Low line heart rates were significantly higher than both Randoms and Highs, indicative of even greater reactivity to novelty. Note that neither High nor Low line heart rates returned to baseline during recovery in the home cage, indicating continuing reactivity to the stressor in both lines, compared to the drop in Random line heart rates.
Figure 9
Figure 9
Mean (±SEM) heart rates (HRs, in beats per minute [bpm]) of Postnatal Day (P) 18 control pups in the High-USV, Low-USV, and Random-USV lines, from Baseline in the home cage (Home Cage, left) through Isolation in a novel cage (Novel Cage, middle), (more ...)
In determining relative influences of sympathetic or parasympathetic systems on heart rates in animals, each branch can be blocked directly, pharmacologically, revealing the influence of the other in its absence. Since the sympathetic nervous system increases heart rate via adrenergic influences on the heart, in this study adrenergic action was blocked with atenolol, a cardio-specific beta-adrenergic antagonist used to block the influence of sympathetic activity (and sympathoadrenal effects). In contrast, the parasympathetic nervous system reduces heart rates via cholinergic vagus nerve activation, therefore atropine, a quarternized muscarinic antagonist with minimal central effects, was used to block parasympathetic activity. Pharmacological blockade revealed that in High line juveniles higher heart rates during isolation stress were the result of greater sympathetic acceleratory influence on heart rates. These findings are consistent with populations of children prone to anxiety [30]. The even higher heart rates of Low line juveniles during isolation stress were clearly due to greater parasympathetic withdrawal, largely eliminating the only braking influence on rising heart rates; however, some sympathetic influences increasing Low line heart rates beyond controls could not be ruled out in this study.
Adult males demonstrated continuity with juvenile heart rate responses during 30 minutes of restraint stress [137]. As shown in Figure 10A, in the first 10 minutes heart rates in High and Low adult males were significantly higher than Random line males; but as animals habituated to restraint, High and Random heart rates declined similarly over time. In contrast, Low line males maintained their high heart rates for the entire 30 minutes. High-frequency variability (r-MSSD [108]) was lower in the Low line males throughout the 30-minute period (Figure 10B), indicative of lower parasympathetic (vagal) restraint on heart rate. Post hoc tests demonstrated that high-frequency variability in Lows was low than both Highs and Random males, consistent with a lack parasympathetic restraint on heart rate in Low line rats. At the same time, blood pressure variability was greater in Low line adult males, a condition that predicts later elevated blood pressure [142].
Figure 10
Figure 10
A. Mean (±SEM) adult male heart rates over 30-min of restraint at 10-min intervals. Significant Line effect (p=.013), which post hocs demonstrate are due to Low > Random line heart rates. Significant Line × Epoch effect (p = .05) (more ...)
Although only suggestive, early life alterations in DA and 5-HT transmission patterns in the PAG and the BNST may have had considerable effects on fine-tuning juvenile and adult cardioregulatory processes. Both of these central structures are involved in autonomic and cardiovascular responsiveness, and particularly the PAG is the final afferently-regulated medial structure before more automatic processing occurs in brain stem [180].
However striking, this finding is not consistent with an earlier study of adult High, Random and Low line males heart rates tested under the same conditions of restraint. In that study, both High and Low males maintained higher heart rates for the entire 30 min, suggesting that both selected lines were hyper-reactive to the confines of restraint [24]. The reasons for the discrepancy between the two studies may be due to a variety of factors, among them handling experience, temperature, to differences in sleep state, or as a consequence of struggling more during restraint. To control for extraneous variables on heart rate, currently we are studying heart rate differences in adult males under anesthesia, again using atenolol and atropine to blockade sympathetic and parasympathetic nervous systems influences on cardiac and blood pressure function.
Although higher sympathetic activation of heart rate is common in children and adults suffering from anxiety disorders (e.g., [67, 179]) greater parasympathetic withdrawal, sometimes combined with higher sympathetic regulation of heart rate is characteristic of patients suffering from depressive disorders [1, 2 159, 160, 178]. Thus it is possible that High line adults will exhibit either the same sympathetic over-activity that characterizes High line juveniles, or lower parasympathetic activity, or both, in line with their anxious/depressed lifetime profile. Conversely, Low line cardiovascular functioning is expected to be characterized primarily by parasympathetic under-activity, consistent with their low anxiety profile, and paralleling human populations exhibiting lifelong aggression/hostility [141, 142].
As noted above, another potential component of a Low line model suggested by autonomic and behavioral data would be elevated aggressive behavior. To test this hypothesis we paired Low line adult males with novel partners of the same line and compared them to comparably-treated males in the Random line, in a novel but neutral, non-threatening environment (Social Interaction test [55]). Since rats are not generally aggressive in this context, males of both lines were previously socially isolated in home cages for 2 weeks, a procedure that increases behavioral reactivity and aggression in about 20 – 30% of rats, depending upon strain (e.g., [175]). As shown in Table 1, about a third of Random line rats responded to this treatment with increases in aggression, that is, within the range observed in other outbred rat strains. In contrast, more than 70% of Low line pairs treated in this way engaged in fights and exhibited freezing behavior associated with fighting. These results suggest that higher than normal aggression in male-male encounters after social isolation is indeed highly characteristic of the Low line.
Table 1
Table 1
Percent (number) of Low-Low and Random-Random adult male pairs fighting or freezing (unpublished data).
In rats, social isolation decreases allopregnanolone levels by 60-70% in cerebral cortex, hippocampus and blood plasma [136]. Decreased frontal cortex allopregnanolone is associated with reductions in 5-alpha-reductase-I, the enzyme involved in the rate-limiting step in the biosynthesis of allopregnanolone from progesterone [99]. This isolation-induced down-regulation can be reversed by applications of low concentrations of allopregnanolone [8, 9]. However, when exposed to an acute stressor like footshock, isolated animals show significant increases in brain and plasma concentrations of allopregnanolone over baseline and over those of socially-housed controls [8,9]. Pretreatment before social interaction with diazepam, a GABAA modulator at the benzodiazepine receptor site, reduces aggressive behavior in isolated rats in a dose-response fashion [40, 175]. Thus, in rodents social isolation produces baseline reduction in allopregnanolone and hence GABAA function, with an apparent rebound in allopregnanolone levels in the face of stress that may mediate increases in aggressive behavior. Other studies have shown that lower levels of GABA are found in mice and rats that are genetically predisposed to aggression or exhibit more aggression under experimental manipulation, suggesting that some individuals are more at risk [100].
Given that brain allopregnanolone levels are higher in Low line animals in response to a stressor [184], one possible scenario underlying higher aggression in socially isolated Low line males is that brain allopregnanolone levels are reduced by social isolation below that of Random line control males. In response to subsequent interaction with strange conspecifics Low line brain allopregnanolone overshoots comparable rises seen in similarly isolated Random males, essentially disinhibiting aggression via GABAA receptor mechanisms [9, 19].
It appears then, that selective breeding for high and low rates of vocalization in response to maternal separation has produced two distinct temperamental styles that include alterations in behavioral, physiological, and neurochemical characteristics. The integrity of these personal styles are continuous across the life span, and express themselves as adult phenotypes comparable to those seen in human populations. In humans and in the selected lines, the origins of behavioral predispositions appear to lie early in development, and consist of unique separation anxiety and attachment responses. Concomitantly, system-wide alterations of physiology are associated with and are part of these overall phenotypes.
In this respect, the High and Low lines share traits distinguishing other animal models selected for “high’ or “low” values of systems associated with anxiety/depression or of aggression/impulsivity (e.g., Wistar-Kyoto, Spontaneously Hypertensive Rats; High Anxiety Behavior, Low Anxiety Behavior rats; [111, 126, 131]). Such clusters of behavioral and physiological traits (endophenotypes [66]) are observed in other species including humans, that mark “passive” and “active” coping styles in response to environmental demands [14, 45, 48, 74, 86, 143, 144; 150]. Recently, this notion has been placed in the context of Darwinian evolutionary theory [87], in which species-wide selection pressures (ultimate and proximate factors) favor preservation of genes modulating endophenotypes associated with each coping style [3, 149]. In a given population, the function of these processes is to maintain organismic stability (allostasis) in adaptation to divergent or extreme environmental demands. Each coping style may be of benefit in a specific type of environment, but when expressed as extreme variants (e.g., [163]), may ultimately lead to overwhelming, but different classes of psychological and physiological costs (allostatic load), to chronic or overwhelming stress [87]. In that case, dysregulation of affective states, decreased neurogenesis and increased cell death, metabolic disorders, cardiovascular syndromes and altered inflammatory and immune responses occur that lead to illnesses that are specific to each style [87].
It is not clear how closely assemblages of endophenotypes displayed by the Low and High lines will conform to the dimensions of behavior and physiology attributed to “active” and “passive” coping styles. It would not be surprising if High and Low lines showed variation in the expression of endophenotypes associated with anxiety or aggression temperaments compared to other such populations. Indeed, it would be surprising if there were not variations in endophenotypes in populations produced by a variety of natural conditions and manipulations, including mouse genetic knock-out technology (e.g., [56, 65, 71]). The High and Low USV lines are the first to demonstrate that these coping styles arise from and are continuous with early life temperamental differences. Moreover, they are the first genetic animal model implicating long-term associations of each style with early attachment responses. As such, they present a potentially powerful model for examining neurobiological gene-environment interactions in the development of life-long affective regulation.
Acknowledgments
Supported by NIMH Grant: R01 MH40430, R03 MH54207, National Alliance for Research in Schizophrenia and Affective Disorders (NARSAD).
1. Agelink MW, Majewski T, Wurthmann C, Postert T, Linka T, Rotterdam S, Klieser E. Autonomic neurocardiac function in patients with major depression and effects of antidepressant treatment with nefazodone. J Affect Disord. 2001 Feb;62(3):187–98. [PubMed]
2. Agelink MW, Boz C, Ullrich H, Andrich J. Relationship between major depression and heart rate variability. Clinical consequences and implications for antidepressant treatment. Psychiatry Res. 2002 Dec 15;113(12):139–49. [PubMed]
3. Ahmadiyeh N, Churchill GA, Shimomura K, Solberg LC, Takashi JS, Redi EE. X-linked and lineage-dependent inheritance of coping responses to stress. Mammal Gen. 2003;14(11):748–757. [PubMed]
4. Allen MT, Matthews KA, Kenyon KL. The relationships of resting baroreflex sensitivity, heart rate variability and measures of impulse control in children and adolescents. Intern J Psychophysiol. 2000;37:185–194. [PubMed]
5. Almeida SS, De Araujo M. Postnatal protein malnutrition affects play behavior and other social interactions in juvenile rats. Physiol Behav. 2001;74:45–51. [PubMed]
6. Almeida SS, Tonkiss J, Galler JR. Prenatal malnutritiion affects the social interactions of juvenile rats. Physiol Behav. 1996;60(1):197–201. [PubMed]
7. Ansorge MS, Zhou M, Lira A, Hen R, Gingrich JA. Early-life blockade of the 5-HT transporter alters emotional behavior in adult mice. Science. 2004;306(5697):792. [PubMed]
8. Barbaccia ML, Roscetti G, Trabucchi M, Mostallino MC, Concas A, Purdy RH, Biggio G. Time-dependent changes in rat brain neuroactive steroid concentrations and GABAA receptor function after acute stress. Neuroendocrinology. 1996 Feb;63(2):166–72. [PubMed]
9. Barbaccia ML, Roscetti G, Trabucchi M, Purdy RH, Mostallino MC, Concas A, Biggio G. The effects of inhibitors of GABAergic transmission and stress on brain and plasma allopregnanolone concentrations. Br J Pharmacol. 1997 Apr;120(8):1582–8. [PubMed]
10. Beatty WW. Hormonal organization of sex differences in play fighting and spatial behavior. In: De Vries GJ, editor. Progr Br Res. Vol. 61. 1983. pp. 315–330. [PubMed]
11. Beique JC, Campbell B, Perring P, Hamblin MW, Walker P, Mladenovic L, Andrade R. Serotonergic regulation of membrane potential in developing rat prefrontal cortex: coordinated expression of 5-hydroxytryptamine 5-HT1A, 5-HT2A, and 5-HT7 receptors. J Neurosci. 2004;24(20):4807–17. [PubMed]
12. Beique JC, Chapin-Penick EM, Mladenovic L, Andrade R. Serotonergic facilitation of synaptic activity in the developing rat prefrontal cortex. J Physiol. 2004;556(Pt 3):739–54. [PubMed]
13. Belsky J, Fearon RM. Infant-mother attachment security, contextual risk, and early development: A moderational analysis. Dev Psychopath. 2002;14(2):239–310. [PubMed]
14. Benus RF, Bohus B, Koolhaas JM, van Oortmerssen GA. Heritable variation for aggression as a reflection of individual coping strategies. Experientia. 1991;47(10):1008–1019. [PubMed]
15. Berger MA, Barros VG, Sarchi MI, Tarazi FI, Antonelli MC. Long-term effects of prenatal stress on dopamine and glutamate receptors in adult rat brain. Neurochem Res. 2002;27(11):1525–33. [PubMed]
16. Biederman J, Rosenbaum JF, Hirshfeld MA, Faraone SV, Bolduc EA, Gersten M. Psychiatric correlates of behavioral inhibition in young children of parents with and without psychiatric disorders. Arch Gen Psychiat. 1990;47:21–26. [PubMed]
17. Biederman J, Rosenbaum JF, Bolduc-Murphy EA, Faraone SV, Chaloff J, Hirshfeld DR, Kagan J. A 3-year follow-up of children with and without behavioral inhibition. J Am Acad Child Adolesc Psychiat. 1993;32(4):814–21. [PubMed]
18. Biederman J, Faraone SV, Milberger S, Jetton JG, Chen L, Mick E, Greene RW, Russell RL. Is childhood oppositional defiant disorder a precursor to adolescent conduct disorder? Findings from a 4-year follow-up study of children with ADHD. J Am Acad Child Adolesc Psychiatr. 1996;35(9):1193–1204. [PubMed]
19. Biggio G. The effects of inhibitors of GABA-ergic transmission and stress on brain and plasma allopregnanolone concentrations. Br J Pharmacol. 1997 Apr;120(8):1582–8. [PubMed]
20. Bitran D, Hilvers RJ, Kellogg CK. Anxiolytic effects of 3 alphahydroxy-5 alpha[beta]-pregnan-20-one: endogenous metabolites of progesterone that are active at the GABAA receptor. Br Res. 1991;561:157–61. [PubMed]
21. Blass EM, Kehoe P. Behavioral characteristics of emerging opioid systems in newborn rats. In: Krasnegor A, Blass EM, Hofer MA, Smotherman WP, editors. Perinatal Development: A Psychobiological Perspective. New York: Academic Press; 1987. pp. 61–82.
22. Bowlby J. Attachment Attachment and Loss. Vol. 1 New York: Basic Books; 1969.
23. Brot MD, Akwa Y, Purdy RH, Koob GF, Britton KT. The anxiolytic-like effects of the neurosteroid allopregnanolone: interactions with GABA(A) receptors. Euro J Pharmacol. 1997;325:1–7. [PubMed]
24. Brunelli SA. Selective breeding for an infant phenotype: Rat pup ultrasonic vocalization (USV) Beh Gen. 2005;35(1):53–65. [PubMed]
25. Brunelli SA, Hofer MA. Selective breeding for an infantile phenotype (isolation calling): A window on developmental processes. In: Blass EM, editor. Handbook of Behavioral Neurobiology: Developmental Psychobiology. Vol. 13. New York: Kluwer Academic/Plenum; 2001. pp. 433–482.
26. Brunelli SA, Kehoe P. Differences in catecholamine utilization at postnatal day 10 in rats selected for ultrasonic vocalization (USV) responses to maternal separation. Abstr Dev Psychobiol. 2005;47(4):421.
27. Brunelli SA, Myers MM, Asekoff SL, Hofer MA. Effects of selective breeding for infant rat ultrasonic vocalization on cardiac responses to isolation. Behav Neurosci. 2002 Aug;116(4):612–23. [PubMed]
28. Brunelli SA, Nie R, Whipple C, Winiger V, Hofer MA, Zimmerberg B. The effects of selective breeding for infant ultrasonic vocalizations on play behavior in juvenile rats. Physiol Behav. 2006;87(3):527–36. [PubMed]
29. Brunelli SA, Vinocur DD, Soo-Hoo D, Hofer MA. Five generations of selective breeding for ultrasonic vocalization (USV) responses in N:NIH strain rats. Dev Psychobiol. 1997;31:255–265. [PubMed]
30. Brunelli SA, Hofer MA, Weller A. Selective breeding for infant vocal response: A role for postnatal maternal effects? Dev Psychobiol. 2001;38:221–228. [PubMed]
31. Burgess KB, Marshall PJ, Rubin KH, Fox NA. Infant attachment and temperament as predictors of subsequent externalizing problems and cardiac physiology. J Child Psychol Psychiatr. 2003;44(6):819–832. [PubMed]
32. Calkins SD, Fox NA. The relations among infant temperament, security of attachment, and behavioral inhibition at twenty-four months. Child Dev. 1992;63(6):1456–1472. [PubMed]
33. Carboni E, Wieland S, Lan NC, Gee KW. Anxiolytic properties of endogenously occurring pregnanediols in two rodent models of anxiety. Psychopharm. 1996;126:173–8. [PubMed]
34. Caspi A, Harrington H, Milne B, Amell JW, Theodore RF, Moffitt TE. Children's behavioral styles at age 3 are linked to their adult personality traits at age 26. J Personal. 2003;71(4):495–513. [PubMed]
35. Caspi A, McLay J, Moffitt TE, Mill J, Marin J, Craig IW, Taylor A, Poulton R. Role of genotype in the cycle of violence in maltreated children. Science. 2002;297(5582):851–854. [PubMed]
36. Caspi A, Moffitt TE, Newman DL, Silva PA. Behavioral observations at age 3 years predict adult psychiatric disorders. Longitudinal evidence from a birth cohort. Arch Gen Psychiatr. 1996;53(11):1033–1039. [PubMed]
37. Caspi A, Sugden K, Moffitt TE, Taylor A, Craig IW, Harrington H, McClay J, Mill J, Martin J, Braithwaite A, Poulton R. Influence of life stress on depression: Moderation by a polymorphism in the 5-HTT gene. Science. 2003;301(5631):386–389. [PubMed]
38. Chen JC, Turiak G, Galler J, Volicer L. Postnatal changes of brain monoamine levels in prenatally malnourished and control rats. Int J Dev Neurosc. 1997;15(2):257–63. [PubMed]
39. Cho J, Kholodilov NG, Burke RE. Patterns of developmental mRNA expression of neurturin and GFRalpha2 in the rat striatum and substantia nigra do not suggest a role in the regulation of natural cell death in dopamine neurons. Br Res: Devl Br Res. 2004;48(1):143–9. [PubMed]
40. Concas A, Biggo C. The effects of inhibitors of GABA-ergic transmission and stress on brain and plasma allopregnanolone concentrations. Br J Pharmacol. 1997;120:1582–8. [PubMed]
41. Cooper PJ, Fearn V, Willetts L, Seabrook H, Parkinson M. Affective disorders in the parents of a clinical sample of children with anxiety disorders. J Affect Disord. 2006 Jul;93(13):205–12. [PubMed]
42. Dallaire DH, Weinraub M. Predicting children's separation anxiety at age 6: the contributions of infant-mother attachment security, maternal sensitivity, and maternal separation anxiety. Att Hum Dev. 2005;7(4):393–408. [PubMed]
43. D'Amato RJ, Blue ME, Largent BL, Lynch DR, Ledbetter DJ, Molliver ME, Snyder SH. Ontogeny of the serotonergic projection to rat neocortex: transient expression of a dense innervation to primary sensory areas. Proc Natl Acad Sci U S A. 1987;84(12):4322–6. [PubMed]
44. Davis M. Neural systems involved in fear and anxiety measured with fear-potentiated startle. Am Psychol. 2006;61(8):741–56. [PubMed]
45. DeBoer SF, van der Vegt BJ, Koolhaas JM. Individual variation in aggression of feral rodent strains: A standard for the genetics of aggression and violence? Beh Gen. 2003;33(5):485–501. [PubMed]
46. De Bruin JP. Social behaviour and the prefrontal cortex. Prog Brain Res. 1990;85:485–96. [PubMed]
47. Dinopoulos A, Dori I, Parnavelas JG. The serotonin innervation of basal forebrain shows a transient phase during development. Dev Brain Res. 1997;99:38–52. [PubMed]
48. Ebner K, Wotjak CT, Landgraf R, Engelmann M. Neuroendocrine and behavioral response to social confrontation: residents versus intruders, active versus passive coping styles. Horm Behav. 2005;47(1):14–21. [PubMed]
49. Einon DF, Morgan MJ. Brief periods of socialization and later behavior in the rat. Dev Psychobiol. 1978;11:213–225. [PubMed]
50. Einon DF, Morgan MJ. A critical period for social isolation in the rat. Dev Psychobiol. 1977;10:123–132. [PubMed]
51. Fagen RM. Animal Play Behaviour. Oxford University Press; New York: 1981.
52. Faraone SV, Biederman J, Keenan K, Tsuang MT. Separation of DSM-III attention deficit disorder and conduct disorder: evidence from a family-genetic study of American child psychiatric patients. Psychol Med. 1991;21(1):109–121. [PubMed]
53. Farrington DP. Early predictors of adolescent aggression and adult violence. Viol Vict. 1989;4(2):79–100. [PubMed]
54. Field EF, Pellis SM. Differential effects of amphetamine on attack and defense components of play fighting in rats. Physiol Beh. 1994;56(2):325–330. [PubMed]
55. File SE, Seth P. A review of 25 years of the social interaction test. Eur J Pharmacol. 2003;463(13):35–53. [PubMed]
56. Foroud T, Ritchotte A, Spence J, Liu L, Lumeng L, Li TK, Carr LG. Confirmation of alcohol preference quantitative trait loci in the replicate high alcohol drinking and low alcohol drinking rat lines. Psychiatr Genet. 2003;13:155–161. [PubMed]
57. Fox NA, Nichols KE, Henderson HA, Rubin K, Schmidt L, Hamer D, Ernst M, Pine DS. Evidence for a gene-environment interaction in predicting behavioral inhibition in middle childhood. Psychol Sci. 2005;16(12):921–6. [PubMed]
58. Francis DD, Szegda K, Campbell G, Martin WD, Insel TR. Epigenetic sources of behavioral differences in mice. Nat Neurosci. 2003;6(5):445–6. [PubMed]
59. Frye CA, Petralia SM, Rhodes ME. Estrous cycle and sex differences in performance on anxiety tasks coincide with increases in hippocampal progesterone and 3-alpha,5alpha-THP. Pharmacol Biochem Behav. 2000;67(3):587–96. [PubMed]
60. Frye CA, Walf AA. Changes in progesterone metabolites in the hippocampus can modulate open field and forced swim test behavior of proestrous rats. Horm Behav. 2002;41:306–15. [PubMed]
61. Frye CA, Walf AA. Estrogen and/or progesterone administered systemically or to the amygdala can have anxiety-reducing, fear-reducing, and pain-reducing effects in ovariectomized rats. Behav Neurosci. 2004;118:306–13. [PubMed]
62. Galineau L, Kodas E, Guilloteau D, Vilar MP, Chalon S. Ontogeny of the dopamine and serotonin transporters in the rat brain: an autoradiographic study. Neurosci Lett. 2004 Jun 17;363(3):266–71. [PubMed]
63. Gaspar P, Cases O, Maroteaux L. The developmental role of serotonin: news from mouse molecular genetics. Nature Rev Neurosci. 2003;4:1002–1012. [PubMed]
64. Gerardin DC, Pereira OC, Kempinas WG, Florio JC, Moreira EG, Bernardi MM. Sexual behavior, neuroendocrine, and neurochemical aspects in male rats exposed prenatally to stress. Physiol Behav. 2005;84(1):97–104. [PubMed]
65. Gingrich JA, Hen R. Dissecting the role of the serotonin system in neuropsychiatric disorders using knockout mice. Psychopharm (Ber) 2001;155(1):1–10. [PubMed]
66. Gould TD, Gottesman II. Psychiatric endophenotypes and the development of valid animal models. Genes, Brain Behav. 2006;5(2):113–119. [PubMed]
67. Greaves-Lord K, Ferdinand RF, Sondeijker FE, Dietrich A, Oldehinkel AJ, Rosmalen JG, Ormel J, Verhulst FC. Testing the tripartite model in young adolescents: Is hyperarousal specific for anxiety and not depression? J Affect Disord. 2007 artcle in press: epub prior to print. [PubMed]
68. Griffith LD, Mellon SH. Selective serotonin reuptake inhibitors directly alter activity of neurosteroidogenic enzymes. Proc Natl Acad Sci USA. 1999 Nov 9;96:13512–17. [PubMed]
69. Haberstick BC, Schmitz S, Young SE, Hewitt JK. Contributions of genes and environments to stability and change in externalizing and internalizing problems during elementary and middle school. Beh Gen. 2005;35(4):381–386. [PubMed]
70. Hahn ME, Hewitt JK, Schanz N, Weinreb L, Henry A. Genetic and developmental influences on infant mouse ultrasonic calling. I. A diallel analysis of the calls of 3-day olds. Beh Gen. 1997;27(2):133–43. [PubMed]
71. Hanilovic D, Cicin-Sain L, Bordukalo-Niksic T, Jernej B. Rats with constitutionally upregulated/downregulated platelet 5HT transporter: differences in anxiety-related behavior. Behav Brain Res. 2005;165(2):271–277. [PubMed]
72. Harvey AT, Hennessy MB. Corticotropin-releasing factor modulation of the ultrasonic vocalization rate of isolated rat pups. Br Res: Dev Br Res. 1995;87(2):125–34. [PubMed]
73. Hennessy MM, Weinberg J. Adrenocortical activity during conditions of brief social separation in preweaning rats. Beh Neur Biol. 1990;54(1):42–55. [PubMed]
74. Henry JP, Stephens PM, Santisteban GA. A model of psychosocial hypertension showing reversibility and progression of cardiovascular complications. Circ Res. 1975;36(1):156–64. [PubMed]
75. Hill J. Biological, psychological and social processes in the conduct disorders. J Ch Psychol Psychiat. 2002;43(1):133–164. [PubMed]
76. Hofer MA. Multiple regulators of ultrasonic vocalization in the infant rat. Psychoneuroendocr. 1996;21(2):203–17. [PubMed]
77. Insel TR, Winslow JT. Anim Models Pharmacol: Adv Pharmacol Sci. Basel: Birkhaauser Verlag; 1991. Rat pup ultrasonic vocalizations: An ethologically relevant behavior responsive to anxiolytics; pp. 15–36.
78. Kagan J, Reznick JS, Gibbons J. Inhibited and uninhibited types of children. Child Dev. 1989;60:838–845. [PubMed]
79. Kagan J, Snidman N, Zentner M, Peterson E. Infant temperament and anxious symptoms in school age children. Dev Psychopathol. 1999;11(2):209–224. [PubMed]
80. Kehoe P, Mallinson K, Bronzino J, McCormick CM. Effects of prenatal protein malnutrition and neonatal stress on CNS responsiveness. Br Res: Dev Br Res. 2001;132(1):23–31. [PubMed]
81. Knutson BJ, Burgdorf J, Panksepp J. Anticipation of play elicits high-frequency ultrasonic vocalizations in young rats. J Comp Psychol. 1998;112(1):65–73. [PubMed]
82. Knutson BJ, Burgdorf J, Panksepp J. High-frequency ultrasonic vocalizations index conditioned pharmacological reward in rats. Physiol Behav. 1999;66(4):639–643. [PubMed]
83. Kochanska G. Mother-child relationship, child fearfulness, and emerging attachment: a short-term longitudinal study. Dev Psychol. 1998;34(3):480–490. [PubMed]
84. Kochanska G. Emotional development in children with different attachment histories: the first three years. Child Dev. 2001;72(2):474–490. [PubMed]
85. Kofman O. The role of prenatal stress in the etiology of developmental behavioral disorders. Neurosci & Biobehav Rev. 2002;26:457–470. [PubMed]
86. Koolhaas JM, Korte SM, De Boer SF, Van der Vegt BJ, Van Reenen CG, Hopster H, De Jong IC, Ruis MA, Blokhuis HJ. Coping styles in animals: Current status in behavior and stress physiology. Neurosci Biobehavl Rev. 1999;23(7):925–935. [PubMed]
87. Korte SM, Koolhaas JM, Wingfield JC, McEwen BS. The Darwinian concept of stress: benefits of allostasis and costs of allostatic load and the treade-offs in health and disease. Neurosci Biobehav Rev. 2005;29:3–38. [PubMed]
88. LaGraize SC, Borzan J, Peng B, Fuchs PN. Selective regulation of pain affect following activation of the opioid anterior cingulate cortex system. Exper Neurol. 2006;197(1):22–30. [PubMed]
89. Landgraf R, Wigger A, Holsboer F, Neumann ID. Hyper-reactive hypothalamo-pituitary-adrenocortical axis in rats bred for high anxiety-related behaviour. J Neuroendocr. 1999;11(6):405–7. [PubMed]
90. Lemaire V, Koehl M, Le Moal M, Abrous DN. Prenatal stress produces learning deficits associated with an inhibition of neurogenesis in the hippocampus. Proc Nat Acad Sci. 2000;97(20):11032–7. [PubMed]
91. Leman S, Dielenberg RA, Carrive P. Effect of dorsal periaqueductal gray lesion on cardiovascular and behavioural responses to contextual conditioned fear in rats. Beh Br Res. 2003;143(2):169–76. [PubMed]
92. Levitsky DA, Barnes RH. Nutritional and environmental interactions in behavioral development of the rat: Long-term effects. Science. 1972;176:68–71. [PubMed]
93. Li QH, Nakadate K, Tanaka-Nakadate S, Nakatsuka D, Cui Y, Watanabe Y. Unique expression patterns of 5-HT2A and 5-HT2C receptors in the rat brain during postnatal development: Western blot and immunohistochemical analyses. J Comp Neurol. 2004;469(1):128–40. [PubMed]
94. MacLean PD, Newman JD. Role of midline frontolimbic cortex in production of the isolation call of squirrel monkeys. Br Res. 1988;450(12):111–23. [PubMed]
95. Manassis K, Bradley S, Goldberg S, Hood J, Swinson RP. Behavioural inhibition, attachment and anxiety in children of mothers with anxiety disorders. Can J Psychiatry. 1995 Mar;40(2):87–92. [PubMed]
96. Mangelsdorf SC, Frosch CA. Temperament and attachment: One construct or two? Adv Child Dev Behav. 1999;27:181–220. [PubMed]
97. Marshall PJ, Stevenson-Hinde J. Behavioral inhibition, heart period, and respiratory sinus arrhythmia in young children. Dev Psychobiol. 1998 Nov;33(3):283–92. [PubMed]
98. Meaney MJ, Stewart J. A descriptive study of social development in the rat (Rattus norvegicus) An Beh. 1981;29:34–45.
99. Mellon SH. Synthesis, enzyme localization, and regulation of neurosteroids. In: Smith SS, editor. Neurosteroid Effects in the Central Nervous System: The Role of the GABAA Receptor. Boca Raton: CRC Press; 2004. pp. 1–46.
100. Miczek KA, Faccidomo S, Almeida RM, Bannai M, Fish EW, Debold JF. Escalated aggressive behavior: new pharmacotherapeutic approaches and opportunities. Ann New York Acad Science. 2004;1036:336–55. [PubMed]
101. Moll GH, Mehnert C, Wicker M, Bock N, Rothenberger A, Ruther E, Huether G. Age-associated changes in the densities of presynaptic monoamine transporters in different regions of the rat brain from early juvenile life to late adulthood. Br Res Devl Br Res. 2000;119(2):251–7. [PubMed]
102. Monk C, Fifer WP, Myers MM, Sloan RP, Trien L, Hurtado A. Maternal stress responses and anxiety during pregnancy: Effects of maternal heart rate. Dev Psychobiol. 2000;36(1):67–77. [PubMed]
103. Monk C, Myers MM, Sloan RP, Fifer WP. Effects of women's stress-elicited physiological activity and chronic anxiety on fetal heart rate. J Dev Behav Ped. 2003;24(1):32–38. [PubMed]
104. Monk C, Sloan RP, Myers MM, Ellman L, Werner E, Jeon J, Tager F, Fifer WP. Fetal heart rate reactivity differs by women's psychiatric status: An early marker for developmental risk? Am Acad Child Adolesc Psych. 2004;43(3):283–290. [PubMed]
105. Muneoka KT, Takigawa M. A neuroactive steroid, pregnenolone, alters the striatal dopaminergic tone before and after puberty. Neuroendocr. 2002;75(5):288–95. [PubMed]
106. Muneoka K, Nakatsu T, Fuji J, Ogawa T, Takigawa M. Prenatal administration of nicotine results in dopaminergic alterations in the neocortex. Neurotox Terat. 1999;1(5):603–9. [PubMed]
107. Muneoka K, Ogawa T, Kamei K. Prenatal nicotine exposure affects the development of the central serotonergic system as well as the dopaminergic system in rat offspring: involvement of route of drug administrations. Br Res: Dev Br Res. 1997;102(1):117–26. [PubMed]
108. Murphy CA, Sloan RP, Myers MM. Pharmacological responses and spectral analyses of spontaneous fluctuations in heart rate and blood pressure in SHR rats. J Autonom Nerv Syst. 1991;36(3):237–50. [PubMed]
109. Nijsen MJ, Croiset G, Diamant M, De Wied D, Wiegant VM. CRH signaling in bed nucleus of the stria terminalis is involved in stress-induced cardiac vagal activation in conscious rats. Neuropsychopharm. 2001 Jan;24(1):1–10. [PubMed]
110. Oades RD, Sadile AG, Sagvolden T, Viggiano D, Zuddas A, Devoto P, Aase H, Johansen EB, Ruocco LA, Russell VA. The control of responsiveness in ADHD by catecholamines: evidence for dopaminergic, noradrenergic and interactive roles. Dev Sci. 2005;8(2):122–31. [PubMed]
111. Overstreet DH. Behavioral characteristics of rat lines selected for differential hypothermic responses to cholinergic or serotonergic agonists. Beh Gen. 2002;32(5):335–348. [PubMed]
112. Palmer AA, Printz DJ, Butler PD, Dulawa SC, Printz MP. Prenatal protein deprivation in rats induces changes in prepulse inhibition and NMDA receptor binding. Br Res. 2004;996(2):193–201. [PubMed]
113. Panksepp J. The ontogeny of play in rats. Devl Psychobiol. 1981;14(4):327–332. [PubMed]
114. Panksepp J, Normansell L, Cox JF, Siviy SM. Effects of neonatal decortication on the social play of juvenile rats. Physiol Behav. 1994;56:429–443. [PubMed]
115. Pellis SM, Pellis VC. Locomotor-rotational movements in the ontogeny and play of the laboratory rat Rattus norvegicus. Dev Psychobiol. 1983;16(4):269–86. [PubMed]
116. Pellis SM. Agonistic versus amicable targets of attack and defense: Consequences for the origin, function, and descriptive classification of play-fighting. Agg Behav. 1988;14:85–104.
117. Pellis SM, Pellis VC, Whishaw IQ. The role of the cortex in play fighting by rats: developmental and evolutionary implications. Br Beh Evol. 1992;39(5):270–84. [PubMed]
118. Pellis SM, Castaneda E, McKenna MM, Tran-Nguyen LTL, Wishaw IQ. The role of the striatum in organizing sequences of play fighting in neonatally dopamine-depleted rats. Neurosci Lett. 1993;158:13–14. [PubMed]
119. Pellitteri R, Zicca A, Mancardi GL, Savio T, Cadoni A. Schwann cell-derived factors support serotoninergic neuron survival and promote neurite outgrowth. Eur J Histochem. 2001;45(4):367–76. [PubMed]
120. Perrone-Capano C, Di Porzio U. Genetic and epigenetic control of midbrain dopaminergic neuron development. Int J Dev Biol. 2000;44:679–687. [PubMed]
121. Pine DS, Wasserman G, Coplan J, Fried J, Sloan R, Myers MM, Greenhill L, Shaffer D, Parsons B. Serotonergic and cardiac correlates of aggression in children. Ann NY Acad Sci. 1996;794:391–3. [PubMed]
122. Pine DS, Wasserman GA, Miller L, Coplan JD, Bagiella E, Kovelenku P, Myers MM, Sloan RP. Heart period variability and psychopathology in urban boys at risk for delinquency. Psychophys. 1998;35(5):521–529. [PubMed]
126. Ramos A, Mormede P. Stress and emotionality: a multidimensional and genetic approach. Neurosci Biobehav Rev. 1998;22(1):33–57. [PubMed]
127. Reddy D, Kulkarni SK. Differential anxiolytic effects of neurosteroids in the mirrored chamber behavior test in mice. Br Res. 1997;752:61–71. [PubMed]
128. Rosenbaum JF, Biederman J, Bolduc-Murphy EA, Faraone SV, Chaloff J, Hirshfeld DR, Kagan J. Behavioral inhibition in childhood: a risk factor for anxiety disorders. Harv Rev Psych. 1993;1(1):2–16. [PubMed]
129. Roubertoux PL, Martin B, Le Roy I, Beau J, Marchaland C, Perez-Diaz F, Cohen-Salmon C, Carlier M. Vocalizations in newborn mice: genetic analysis. Beh Gen. 1995;26(4):427–437. [PubMed]
130. Rutter M, Moffitt TE, Caspi A. Gene-environment interplay and psychopathology: multiple varieties but real effects. J Child Psychol Psych. 2005;47(34):226–261. [PubMed]
131. Salome N, Viltart O, Lesae J, Landgraf R, Vieau D, Laborie C. Altered hypothalamo-pituitary-adrenal sympatho-adrenomedullary activities in rats bred for high anxiety: central and peripheral correlates. Psychoneur. 2006;31:724–735. [PubMed]
132. Schmidt LA, Fox NA, Rubin KH, Hu S, Hamer DH. Molecular genetics of shyness in preschoolers. Pers Indiv Diff. 2002;33:227–238.
133. Schwartz CE, Snidman N, Kagan J. Adolescent social anxiety as an outcome of inhibited temperament in childhood. J Am Acad Child Adol Psych. 1999;38(8):1008–1015. [PubMed]
134. Schwartz CE, Wright CZ, Shin LM, Kagan J, Rauch SL. Inhibited and uninhibited children “grown up”: adult amygdalar response to novelty. Science. 2003;300(5627):1952–1953. [PubMed]
135. Schneider KM, Nicolotti L, Delamater A. Aggression and cardiovascular response in children. J Ped Psychol. 2002;27(7):565–573. [PubMed]
136. Serra M, Pisu MG, Littera M, Papi G, Sanna E, Tuveri F, Usala L, Purdy RH, Biggio G. Social isolation-induced decreases in both the abundance of neuroactive steroids and GABA-A receptor function in rat brain. J Neurochem. 2000;75:732–740. [PubMed]
137. Shair HN, Brunelli SA, Velazquez Z, Hofer MA. Adult behavioral tests of rats selectively bred for infantile ultrasonic vocalization. Abst Dev Psychobiol. 2002;356
138. Shamir-Essakow G, Ungerer JA, Rapee RM. Attachment, behavioral inhibition, and anxiety in preschool children. J Abnorm Child Psychol. 2005;33(2):131–143. [PubMed]
139. Siviy SM, Baliko CN, Bowers KS. Rough-and-tumble play behavior in Fischer-344 and Buffalo rats: effects of isolation. Physiol Behav. 1997;61:597–602. [PubMed]
140. Siviy SM, Love NJ, DeCicco BM, Giordano SB, Seifert TL. The relative playfulness of juvenile Lewis and Fischer-344 rats. Physiol Behav. 2003;80(23):385–394. [PubMed]
141. Sloan RP, Shapiro PA, Bigger JT, Bagiella E, Steinman RC, Gorman JM. Cardiac autonomic control and hostility in healthy subjects. Am J Cardiol. 1994;74:298–300. [PubMed]
142. Sloan RP, Shapiro PA, Bagiella E, Myers MM, Gorman JM. Cardiac autonomic control buffers blood pressure variability responses to challenge: A psychophysiological model of coronary artery disease. Psychosom Med. 1999;51:58–68. [PubMed]
143. Sluyter F, Korte SM, Bohus B, van Oortmerssen GA. Behavioral stress responses of genetically selected aggressive and nonagressive wild house mice in the shock-probe/defensive burying test. Pharm Biochem Beh. 1995;54(1):113–116. [PubMed]
144. Sluyter F, Arseneault L, Moffitt TE, Veenema AH, de Boer S, Koolhaas JM. Toward an animal model for antisocial behavior: parallels between mice and humans. Beh Gen. 2003;33(5):563–574. [PubMed]
145. Smith SS. Withdrawal effects of a neuroactive steroid as a model of PMS: Synaptic physiology to behavior. In: Smith SS, editor. Neurosteroid Effects in the Central Nervous System: The Role of the GABAA Receptor. Boca Raton, FLA: CRC Press; 2004. pp. 144–172.
146. Smoller JW, Rosenbaum JF, Biederman J, Kennedy J, Dai D, Racette SR, Laird NM, Kagan J, Snidman N, Hirshfeld-Becker D, Tsuang MT, Sklar PB, Slaugenhaupt SA. Association of a genetic marker at the cortiocotrophin-releasing hormone locus with behavioral inhibition. Biol Psych. 2003;54(12):1376–1381. [PubMed]
147. Smoller JW, Yamaki LH, Fagerness JA, Biederman J, Racette SR, Laird NM, Kagan J, Snidman N, Faraone SV, Hirshfeld-Becker D, Tsuang MT, Slaugenhaupt SA, Rosenbaum JF, Sklar PB. The corticotrophin-releasing hormone gene and behavioral inhibition in children at risk for panic disorder. Biol Psych. 2005 Jun 15;57(12):1485–92. [PubMed]
148. Stams GJ, Juffer F, van Izendoorn MH. Maternal sensitivity, infant attachment, and temperament in early childhood predict adjustment in middle childhood: the case of adopted children and their biologically unrelated parents. Dev Psychol. 2002;38(5):806–821. [PubMed]
149. Steimer T, Driscoll P. Divergent stress responses and coping styles in psychogenetically selected Roman high-(RHA) and low-(RLA) avoidance rats: behavioral, neuroendocrine and developmental aspects. Stress. 2003;6(2):87–100. [PubMed]
150. Stenzel-Poore MP, Heinrichs SC, Rivest S, Koob GF, Vale WW. Overproduction of corticotropin-releasing factor in transgenic mice: a genetic model of anxiogenic behavior. J Neurosci. 1994;14(5 Pt 1):2579–84. [PubMed]
151. Stevenson-Hinde J, Marshall PJ. Behavioral inhibition, heart period, and respiratory sinus arrhythmia: an attachment perspective. Child Dev. 1999;70(4):805–16. [PubMed]
152. Stevenson-Hinde J, Shouldice A. 4.5 to 7 years: fearful behaviour, fears and worries. J Child Psychol Psychiatry. 1995 Sep;36(6):1027–38. [PubMed]
153. Sullivan RM. Hemispheric asymmetry in stress processing in rat prefrontal cortex and the role of mesocortical dopamine. Stress. 2004;7(2):131–43. [PubMed]
154. Sullivan RM, Brake WG. What the rodent prefrontal cortex can teach us about attention-deficit/hyperactivity disorder: the critical role of early developmental events on prefrontal function. Beh Br Res. 2003;146(12):43–55. [PubMed]
155. Sundström-Poromaa I. Premenstrual dysphoric disorder. In: Smith SS, editor. Neurosteroid Effects in the Central Nervous System: The Role of the GABAA Receptor. 12. Vol. 146. Boca Raton, FLA: CRC Press; 2004. pp. 291–304.
156. Tarazi FI, Tomasini EC, Baldessarini RJ. Postnatal development of dopamine and serotonin transporters in rat caudate-putamen and nucleus accumbens septi. Neurosci Lett. 1998 Sep 18;254(1):21–4. [PubMed]
157. Thor DH, Holloway WR., Jr Play-solicitation behavior in juvenile male and female rats. An Learn Beh. 1983;11(2):173–178.
158. Tonkiss J, Smart JL. Interactive effects of genotype and early life undernutrition on the development of behavior in rats. Dev Psychobiol. 1983;16(4):287–301. [PubMed]
159. Tulen JH, Bruijn JA, de Man KJ, van der Velden E, Pepplinkhuizen L, Man in 't Veld AJ. Anxiety and autonomic regulation in major depressive disorder: An exploratory study. J Affect Disord. 1996 Sep 9;40(12):61–71. [PubMed]
160. Tulen JH, Bruijn JA, de Man KJ, Pepplinkhuizen L, van den Meiracker AH, Veld AJ. Cardiovascular variability in major depressive disorder and effects of imipramine or mirtazamine (Org 3770) J Clin Psychopharmacol. 1996 Apr;16(2):135–45. [PubMed]
161. van Broekhoven F, Verkes RJ. Neurosteroids in depression: a review. Psychopharmacology. 2003;165:97–110. [PubMed]
162. Vanderschuren LJMJ, Niesink RJM, Van Ree JM. The neurobiology of social play behavior in rats. Neurosc Biobehav Rev. 1997;21(3):309–326. [PubMed]
163. Veneema AH, Meijer OC, de Kloet ER, Koolhaas JM. Genetic selection for coping style predicts stressor susceptibility. J Neuroendocr. 2003;15(3):256–267. [PubMed]
164. Vivian JA, Barros HM, Maintiu A, Miczek KA. Ultrasonic vocalizations in rat pups: modulation at the gamma-aminobutyric acid A receptor complex and the neurosteroid recognition site. J Pharmacol Exp Therap. 1997;282:318–25. [PubMed]
165. Vivian JA, Miczek KA. Interactions between social stress and morphine in the periaqueductal gray: effects on affective vocal and reflexive pain responses in rats. Psychopharm (Berl) 1999;146(2):153–61. [PubMed]
166. Warner V, Weissman MM, Mufson L, Wickramaratne PJ. Grandparents, parents, and grandchildren at high risk for depression: A three-generation study. J Am Acad Child Adolesc Psych. 1999;38(3):289–296. [PubMed]
167. Warren SL, Gunnar MR, Kagan J, Anders TF, Simmens SJ, Rones M, Wease S, Aron E, Dahl RE, Sroufe LA. Maternal panic disorder: Infant temperament, neurophysiology, and parenting behaviors. J Am Acad Child Adolesc Psych. 2003;42(7):814–825. [PubMed]
168. Weissman MM, Wickramaratne PJ, Nomura Y, Warner V, Verdeli H, Pilowsky DJ, Grilllon C, Bruder G. Families at high and low risk for depression: A 3-generation study. Arch Gen Psych. 2005;62(1):29–36. [PubMed]
169. Weissman MM, Wickramaratne P, Nomura Y, Warner V, Pilowsky D, Verdeli H. Offspring of depressed parents: 20 years later. Am J Psychiatry. 2006 Jun;163(6):1001–8. [PubMed]
170. West MJ, King AP. Settling nature and nurture into an ontogenetic niche. Dev Psychobiol. 1987;20(5):549–62. [PubMed]
171. Whatson TS, Smart JL. Social behavior of rats following pre- and early postnatal undernutrition. Physiol Behav. 1978;20:749–753. [PubMed]
172. Whatson TS, Smart JL, Dobbing J. Dominance relationships among previously undernourished and well fed male rats. Physiol Behav. 1975;14:425–429. [PubMed]
173. Whatson TS, Smart JL, Dobbing J. Undernutrition in early life: Lasting effects on activity and social behavior of male and female rats. Dev Psychobiol. 1976;9(6):529–538. [PubMed]
174. Wickramaratne PJ, Greenwald S, Weissman MM. Psychiatric disorders in the relatives of probands with prepubertal-onset or adolescent-onset major depression. J Am Acad Child Adolesc Psych. 2000;39(11):1396–1405. [PubMed]
175. Wongwitdecha N, Marsden CA. Social isolation increases aggressive behaviour and alters the effects of diazepam in the rat social interaction test. Beh Br Res. 1996;75(12):27–32. [PubMed]
176. Yang H, Chang JY, Woodward DJ, Baccala LA, Han JS, Luo F. Coding of peripheral electrical stimulation frequency in thalamocortical pathways. Exp Neurol. 2005;196(1):138–52. [PubMed]
177. Yeragani VK. Heart rate and blood pressure variability: Implications for psychiatric research. Neuropsychobiol. 1995;32:182–191. [PubMed]
178. Yeragani VK, Rao KA, Pohl R, Jampala VC, Balon R. Heart rate and QT variability in children with anxiety disorders: a preliminary report. Depr Anx. 2001;13:72–77. [PubMed]
179. Yeragani VK, Pohl R, Balon R, Jampala VC, Jayaraman A. Twenty-four-hour QT interval variability: increased QT variability during sleep in patients with panic disorder. Neuropsychobiology. 2002;46(1):1–6. [PubMed]
180. Zhang SP, Davis PJ, Bandler R, Carrive P. Brain stem integration of vocalization: role of the midbrain periaqueductal gray. J Neurophysiol. 1994 Sep;72(3):1337–56. [PubMed]
181. Zhang ZW. Serotonin induces tonic firing in layer V pyramidal neurons of rat prefrontal cortex during postnatal development. J Neurosci. 2003;23(8):3373–84. [PubMed]
182. Zhou FC, Sari Y, Zhang JK. Expression of serotonin transporter protein in developing rat brain. Brain Res Dev Brain Res. 2000 Jan 3;119(1):33–45. [PubMed]
183. Zimmerberg B, Brunelli SA, Hofer MA. Reduction of rat pup ultrasonic vocalizations by the neuroactive steroid allopregnanolone. Pharm Biochem Beh. 47:735–8. 1004. [PubMed]
184. Zimmerberg B, Brunelli SA, Fluty AJ, Frye CA. Differences in affective behaviors and hippocampal allopregnanolone levels in adult rats of lines selectively bred for infantile vocalizations. Beh Br Res. 2005;159(2):301–11. [PubMed]