PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of nihpaAbout Author manuscriptsSubmit a manuscriptNIH Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Nat Genet. Author manuscript; available in PMC Jan 1, 2010.
Published in final edited form as:
Published online May 31, 2009. doi:  10.1038/ng.392
PMCID: PMC2757943
NIHMSID: NIHMS115931
Lin28 Enhances Tumorigenesis and is Associated With Advanced Human Malignancies
Srinivas R. Viswanathan,1 John T. Powers,1 William Einhorn,1 Yujin Hoshida,2,5 Tony Ng,15 Sara Toffanin,8,9 Maureen O'Sullivan,16 Jun Lu,2,3,5 Letha A. Philips,13 Victoria L. Lockhart,12 Samar P. Shah,1 Pradeep S. Tanwar,7 Craig H. Mermel,5 Rameen Beroukhim,5 Mohammad Azam,1 Jose Teixeira,7 Matthew Meyerson, Timothy P. Hughes,14 Josep M Llovet,8,10,11 Jerald Radich,12 Charles G. Mullighan,13 Todd R. Golub,2,5 Poul H. Sorensen,15 and George Q. Daley1,2,3,4*
1Stem Cell Program, Children's Hospital Boston, Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Harvard Stem Cell Institute, Boston, MA 02115, USA.
2Division of Pediatric Hematology/Oncology, Children's Hospital Boston and Dana Farber Cancer Institute, Boston, MA 02115, USA.
3Howard Hughes Medical Institute, Boston, MA 02115, USA.
4Division of Hematology, Brigham and Women's Hospital, Boston, MA 02115, USA.
5Cancer Program, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA
7Massachusetts General Hospital, Vincent Center for Reproductive Biology, Thier 913, 55 Fruit Street, Boston, Massachusetts
8Mount Sinai Liver Cancer Program, Divisions of Liver Diseases, Mount Sinai School of Medicine, New York, NY 10029, USA
9Gastrointestinal Surgery and Liver Transplantation Unit, National Cancer Institute, IRCSS Foundation, Milan 20133, Italy
10BCLC Group [HCC Translational Research Lab, Liver Unit, and Department of Pathology], IDIBAPS, CIBERehd, Hospital Clínic, Barcelona 08036, Catalonia, Spain
11Institució Catalana de Recerca i Estudis Avançats, Barcelona, Catalonia, Spain
12Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle WA, USA
13St Jude Children's Research Hospital, Memphis, TN
14Division of Haematology, The Institute for Medical and Veterinary Science, Adelaide, South Australia 5000, Australia
15Department of Pathology, University of British Columbia, and the Department of Molecular Oncology, BC Cancer Research Centre, Vancouver, BC, Canada V5Z 1L3.
16Department of Pathology, Our Lady's Children's Hospital Crumlin, and Department of Pathology, Trinity College Dublin, Dublin, Ireland
*To Whom Correspondence should be Addressed: George Daley, Phone:(617) 919-2013, Fax:(617) 730-0222, E-mail: george.daley/at/childrens.harvard.edu
Author Contributions
S.R.V. and G.Q.D. conceived experiments and wrote the manuscript. S.R.V., J.T.P., W.E., Y.H., T.N., S.T., M.O., J.L. L.A.P., V.L.L, S.P.S., P.S.T., C.H.M., R.B., M.A., J.T., M.M., T.P.H., J.M.L., J.R, C.G.M., T.R.G. and P.H.S. executed experiments, contributed reagents, analyzed array data, and edited the manuscript.
Multiple members of the let-7 family of miRNAs are often repressed in human cancers1,2, thereby promoting oncogenesis by de-repressing the targets K-Ras, c-Myc, and HMGA2 3,4. However, the mechanism by which let-7 miRNAs are coordinately repressed is unclear. The RNA-binding proteins Lin28 and Lin28B block let-7 precursors from being processed to mature miRNAs58, suggesting that over-expression of Lin28/Lin28B might promote malignancy via repression of let-7. Here we show that LIN28 and LIN28B are over-expressed in primary human tumors and human cancer cell lines (overall frequency ~15%), and that over-expression is linked to repression of let-7 family miRNAs and de-repression of let-7 targets. Lin28/Lin28B facilitate cellular transformation in vitro, and over-expression is associated with advanced disease across multiple tumor types. Our work provides a mechanism for the coordinate repression of let-7 miRNAs observed in a subset of human cancers, and associates activation of LIN28/LIN28B with poor clinical prognosis.
Human tumors display a global reduction in miRNA expression1,2. Inhibition of the miRNA processing pathway via knockdown of miRNA processing enzymes promotes cellular transformation and tumorigenesis; this effect can be attenuated by ectopic expression of let-7g, suggesting that inhibition of let-7 processing is likely to be central to promoting the transformed state3. Low levels of let-7 correlate with increased transformation capacity in vitro3, and have been observed in various malignancies, including lung cancer,9,10 hepatocellular carcinoma11, melanoma12, and ovarian cancer13. Over-expression of let-7 represses cellular proliferation pathways, inhibits cell growth, and impairs tumor development in both xenograft models and in an autochthonous model of non-small cell lung cancer3,14,15,16. Copy number loss3,1719 and epigenetic silencing20 of individual let-7 family members have been reported in human cancers, and such events have been suggested to contribute to the disease state. However, in tissues where let-7 family members are co-expressed, inactivation of individual let-7 miRNAs would be expected to only modestly decrease let-7 dosage, given the functional redundancy among family members. Therefore, we hypothesized that activation of LIN28/LIN28B could serve as a physiological mechanism by which levels of all let-7 family miRNAs are coordinately repressed in the context of human malignancy.
We initially sought to determine whether over-expression of Lin28 could promote cellular transformation in experimental settings. We observed that over-expression of Lin28 in NIH/3T3 cells potently depletes levels of multiple mature let-7 family member miRNAs (Fig. 1a), leading to a concomitant increase in protein levels of c-Myc, a let-7 target (Fig. 1b). NIH/3T3 cells overexpressing Lin28 formed colonies in soft agar (Fig 1c–d) at a frequency comparable to BCR-ABL (Fig. S1) and slightly lower than that reported for c-Myc (1–2%), both of which are weakly transforming in this assay21. Expression of Lin28 together with BCR-ABL resulted in an increase in both colony size and number as compared with either Lin28 or BCR-ABL alone (Fig. S1). NIH/3T3 cells expressing either Lin28 or LIN28B formed tumors in nude mice (Fig. S1b and Table S1). Although the tumors were small and of delayed latency, they displayed evidence of local invasion (Fig. S1b), similar to what has been observed upon global inhibition of miRNA processing3, and consistent with a recent report that Lin28 enhances metastasis22. Co-expression of Lin28 and 7S21L (a let-7 loop mutant capable of being processed to mature let-7 in the presence of Lin2823) abrogated the ability to form colonies in semisolid medium (Fig 1c–d); thus, Lin28 transforms NIH/3T3 cells via the de-repression of let-7 target genes. We also observed that Lin28 over-expression enhanced transformation of a mouse lung adenocarcinoma cell line in which impairment of miRNA processing had been previously reported to be transforming3 (Fig. S2).
Figure 1
Figure 1
Lin28 Transforms NIH/3T3 cells
We next tested Lin28 for its ability to transform cytokine-dependent BaF/3 cells to factor independence. Over-expression of Lin28 in BaF/3 cells potently depleted mature let-7 family members and led to the upregulation of the let-7 targets K-Ras and c-Myc (Fig. 1e–f). Co-expression of Lin28 and a weakly transforming variant of c-SRC (SRC/Y530F) conferred factor independence upon Ba/F3 cells (Fig. 1g–h) and strongly promoted growth in semisolid medium (Fig. 1i). Mice injected with Ba/F3 cells co-expressing Lin28 and Src/Y530F succumbed to leukemic disease more rapidly than mice injected with Ba/F3 cells expressing Src/Y530F alone (Fig. 1j). Lin28 cooperated similarly with a weakly transforming variant of c-Abl (Abl/G2A) to transform Ba/F3 cells (Fig. 1k–m). Notably, BCR-ABL, v-abl, and v-src have all been suggested to transform lymphoid cells via a similar pathway24. Together, these experiments demonstrate that over-expression of Lin28 facilitates cellular transformation in multiple in vitro assays and accelerates disease progression in a murine model.
To determine whether LIN28 and LIN28B are aberrantly expressed in the setting of human cancer, we examined LIN28 expression by microarray analysis of a large panel of human cancer cell lines; we observed expression in 3.2% of cell lines (17/527; Fig. S3a), including lines derived from germ cell tumors, Wilms’ tumor (WT), and hepatocellular carcinomas (HCC). Analysis of microarray data available for the National Cancer Institute panel of 60 cell lines (NCI-60) revealed LIN28 expression in 5% (3/60) and LIN28B expression in 10% (6/60) of the lines (Fig. S3b and Table S2). We also performed quantitative PCR analysis on a select panel of cancer cell lines and found several that express LIN28B, including multiple cell lines derived from patients in the blast crisis phase of chronic myeloid leukemia (CML) (Table S2). Examination of LIN28 expression in a range of primary tumor types by immunohistochemistry on a multi-tumor tissue microarray revealed that LIN28 was highly overexpressed in diverse tumor types including colon cancer, breast cancer, lung cancer, and cervical cancer (Fig. S4 and Table S3). Together, these data show that LIN28/LIN28B are aberrantly expressed in ~15% of surveyed cancer samples.
We surmised that activation of LIN28/LIN28B could represent a physiologic means by which let-7 levels are coordinately repressed in a subset of human cancers, and that over-expression of these factors might modify disease severity. Given that we observed over-expression in cell lines from patients with CML, we examined levels of LIN28 and LIN28B in peripheral blood from patients in either chronic phase (CP) or blast crisis (BC) of CML. LIN28 expression was detected more frequently in patients with BC-CML (42.8%, 6/14) or accelerated phase CML (40%, 4/10) than in patients with CP-CML (9.1%, 1/11) (Fig. 2a). Furthermore, analysis of 13 paired CP-BC samples showed activation of LIN28B upon progression to BC in three patients (23%, 3/13) (Table S4). Therefore LIN28/LIN28B expression is associated with disease progression in a subset of CML patients. Interestingly, we observed that expression of HMGA2, a let-7 target gene, shows a similar relationship with disease progression in CML, as has also been reported elsewhere25 (Fig. S5). This supports the notion that LIN28/LIN28B activation drives progression in CML via let-7 pathways. To determine whether cell lines derived from patients with BC-CML might be dependent on LIN28/LIN28B for growth, we inhibited LIN28B expression in K562 cells by RNA interference. Infection of K562 cells with an shRNA against LIN28B restored levels of mature let-7 miRNAs (Fig. 2b–c), decreased levels of c-MYC (Fig. 2d), impaired proliferative capacity and the ability to form colonies in soft agar (Fig. 2e–f), and resulted in morphological changes suggestive of differentiation (Fig. 2g)26. Similar results were obtained in a second CML-BC cell line and in a lung adenocarcinoma cell line (Fig. S6Fig. S7), both of which express LIN28B.
Figure 2
Figure 2
LIN28B Knockdown Impairs Growth and Triggers Differentiation of K562 Cells
We next sought to determine whether LIN28/LIN28B might be similarly associated with advanced disease in HCC. We performed genome-wide mRNA and microRNA profiling on 89 HCC samples. LIN28B was highly expressed in a subset of HCCs, and these tumors displayed coordinate repression of all let-7 family miRNAs (Fig. 3a). Gene set enrichment analysis revealed that expression of let-7 target genes and myc target genes were significantly enriched in LIN28B-expressing tumors (Fig. 3b), suggesting that LIN28B represses let-7 and functionally activates let-7 targets in the setting of HCC. Tumors with high LIN28B expression accumulated in a molecular subclass defined by a meta-analysis of published transcriptome datasets (YH and TRG, data not shown), which is characterized by high serum alpha-fetoprotein level, higher tumor grade, and c-MYC activation (p=0.006, Fisher’s exact test). Consistently, LIN28B expression was observed in 66% (4/6) of tumors from patients with serum alpha-fetoprotein (AFP) > 10,000 ng/ml as compared to 16.3% (15/92) (p=0.00981, Fisher’s Exact Test). LIN28B-expressing tumors also fell predominantly within a subclass characterized by a proliferative nature as defined by unsupervised clustering27 (p=0.004), and most LIN28B-expressing tumors were of advanced stage (Fig. 3d). Moreover, LIN28B expression was associated with a significantly increased incidence of early recurrence (within 2 years of resection, p=0.02, log-rank test), but was not associated with late recurrence (Fig. 3c). Early recurrence is an indicator of malignant disease while late recurrence is thought to occur as a de novo event from the carcinogenetic microenvironment of the cirrhotic liver28. Knockdown of LIN28B in HepG2 cells (an HCC cell line expressing LIN28B) led to a marked decrease in the number of colonies formed in semisolid medium (Fig. 3e). Together, these results demonstrate that LIN28B is associated with advanced disease and poor clinical outcome in HCC.
Figure 3
Figure 3
LIN28B is associated with poor outcome in hepatocellular carcinoma
To determine whether LIN28/LIN28B are associated with advanced malignancy in other settings, we interrogated published microarray data from multiple tumor types and found high levels of LIN28 and/or LIN28B expression in a discrete subset of human tumors including WT (Fig. S8), ovarian carcinoma (Fig. S9), and germ cell tumors (West et al., submitted). Strikingly, LIN28/LIN28B expression was specifically associated with advanced disease in all of these tumor types. In WT, LIN28/LIN28B expression was only observed in Stage 3 or Stage 4 tumors (p= 0.00361, Fisher’s exact test), but not in lower stage tumors (Fig. S8). Among ovarian carcinomas, LIN28/LIN28B expression was observed predominantly in tumors of histological Grade 2 or 3, rather than Grade 1 (Fig. S9).
Various mechanisms may explain LIN28/LIN28B expression in human cancers, including genomic amplification, aberrant hypomethylation, and expression in a rare cell of origin that acquires additional malignant lesions. Amplification of regions containing the LIN28 (1p36) 29 and LIN28B (6q21) 30,31 loci in human cancers have been reported. We surveyed SNP array data from 3330 primary tumors and cell lines to check for focal copy number changes at the LIN28 and LIN28B loci. Although we observed no appreciable copy number changes at the LIN28 locus, a small subset of cancers showed focal copy number increases of LIN28B (1.7% overall) (Table S5 and Figure S10). However, most amplification events were rare and typically broad, involving both LIN28B and numerous neighboring genes. Analysis of SNP array data in 31 CML-BC samples (22 myeloid, 9 lymphoid) revealed no focal alterations at either the LIN28 or LIN28B loci (data not shown). Thus, while genomic amplification may explain LIN28B expression in a small number of tumors, it is unlikely to be a dominant mechanism of activation. We also assessed methylation status at a CpG island located within the LIN28B locus, and observed relative hypomethylation in LIN28B-expressing cell lines (Fig. S11), suggesting that aberrant hypomethylation may poise the LIN28B locus for transcriptional activation in some tumors. Interestingly, this region of aberrant hypomethylation overlaps with a recently defined c-Myc binding site within the LIN28B locus32. This, together with our observation that LIN28B-expressing tumors cluster in a high-Myc expression subclass, suggests that Myc may transcriptionally activate LIN28B in some contexts.
Notably, there have been several reported cases of WT with a translocation involving 6q213337, the band on which LIN28B is located. To determine whether these translocations may be associated with altered expression of LIN28B, we analyzed a case of sporadic WT with a previously described chromosomal translocation t(6;15)(q21;q21)33,38. Previous mapping by FISH analysis had narrowed the 6q21 breakpoint in this case to a region intergenic to two 6q21 genes, HACE1 and LIN28B, and had demonstrated that loss of HACE1 expression in a cohort of WT cases39 was associated with altered methylation of several CpG islands in the region, including CpG-29 directly adjacent to the LIN28B gene (Fig S12a). To further map the 6q21 breakpoint in this case, we performed Southern blot assay using probes from the intergenic region. A probe covering CpG-29 and flanking sequences lying ~3–4 kb upstream of the LIN28B transcriptional start site demonstrated rearrangement using PstI-digested genomic DNA from the index WT case compared to matching normal kidney and other controls (Figure S12a–c). Moreover, quantitative RT-PCR showed dramatically increased expression of LIN28B in the index case compared to patient-matched normal kidney (Figure S12d). A second WT cause carrying a t(6;15) (Fig. S12e) also displayed dramatically increased expression of LIN28B in tumor sample compared with normal kidney (Fig. S12f). Therefore, LIN28B may be activated by translocation, although the frequency of such events remains to be determined.
Lin28 and Lin28b are expressed in various stem cell populations8,40,41, although there is scarce expression in most terminally differentiated somatic tissues42. Although our data suggest that altered gene expression of LIN28 or LIN28B can promote tumorigenesis and is associated with advanced malignancy, we cannot rule out the possibility that in some settings, LIN28 or LIN28B may be endogenously expressed in a rare cell of origin that develops into an aggressive tumor. We have observed relatively strong LIN28 expression within cells of the normal ovarian surface epithelium, thought to be the cells of origin of epithelial ovarian cancer (Fig. S9)43. Some poor-prognosis HCCs have also been suggested to arise from a primitive cell of origin that shares gene expression patterns with fetal hepatoblasts44.
Recently, LIN28 was used together with OCT4, NANOG, and SOX2 to reprogram human somatic fibroblasts to pluripotency45. Over-expression of other stem cell factors, such as BMI146 and OCT447,48, has been reported to promote oncogenesis by driving self-renewal and proliferation. Moreover, poorly differentiated, aggressive human tumors have recently been shown to have an embryonic stem cell-like gene expression signature49; other embryonic factors have also been reported to have roles in tumor progression50,51. Our data add LIN28/LIN28B to a growing list of embryonic genes involved in oncogenesis, and suggest a mechanism whereby repression of let-7 by LIN28/LIN28B may help to confer self-renewal capacity on cancer stem cells52. Antagonizing LIN28 and/or LIN28B function in tumors that overexpress these genes may provide a means to reactivate the expression of let-7 family tumor suppressors, and may thus be therapeutically beneficial.
Cell Culture
Cell lines were originally obtained from ATCC and cultured under standard conditions. BaF3/AblG2A and Baf3/SrcY530F lines have been described elsewhere54. LKR cells were derived from a mouse adenocarcinoma55 and were a kind gift of Carla Kim.
Antibodies
The following antibodies were used for immunoblotting: c-myc (Santa Cruz, sc-764), KRas (Santa Cruz, sc-30), Abl-K12 (Santa Cruz, sc-131), c-Src (Cell Signaling, #2110). Anti-Lin28 (Proteintech group) was used for immunohistochemistry.
Cloning and Plasmid Construction
Mammalian Lin28 and LIN28B cDNA were subcloned into pBabe.Puro and pMSCV.Neo retroviral vectors. pMSCV.Neo.let-7g construct was previously described3. LIN28B shRNA in lentiviral plasmid was purchased from Sigma-Aldrich (TRCN0000122599). Control shRNA was commercially purchased (SHC002V, Sigma-Aldrich)
Immunohistochemistry
Sections of tissues were deparaffinized with xylene and rehydrated with graded series of ethanol (absolute, 95%, 80% and 50%, respectively, and distilled water), followed by two washes of 5 min each in PBS-T. Antigen retrieval was performed for 20 min in sodium citrate buffer (10mM pH 6) at 90–100°C followed by wash with PBST 1× 5 min. Tissue sections were then incubated for 10 min in 3% (v/v) hydrogen peroxide in methanol to block endogenous peroxidase activity. Sections were then washed for 5 min in PBS-T and blocked at room temperature for 1 h by using 2% normal goat serum, 2% bovine serum albumin (BSA) and 0.1% triton-X in PBS. Tissue sections were then incubated in humidified chamber for overnight incubation at 4°C with primary antibody (1/200 in TBST). Sections were subsequently washed with PBS-T (3× 5min) and incubated at room temperature for 1 h with secondary antibody (goat anti rabbit). After a wash with PBS-T (3× 5min), sections were incubated with ready to use streptavidin peroxidase (Lab Vision, Fremont, CA) for 10 min at room temperature and then color was developed by using a DAB kit (Vector laboratories, Burlingame, CA). Sections were counterstained with hematoxylin.
Viral Production
For ecotropic viral production, retroviral plasmid DNA and pCL-Eco were transfected into 293T cells in a 1:1 mass ratio and virus harvested after 48h. For VSV-G pseudotyped lentivirus, viral plasmid, lentiviral gag/pol, and VSV-G were transfected in a mass ratio 1:0.9:0.1, and virus was harvested after 72 hrs. 1 mL of unconcentrated viral supernatant was used to infect 50,000 cells. Infected cells were selected on antibiotic prior to subsequent analysis.
Bisulfite Sequencing
Bisulfite sequencing was performed as described56 with the commercially available EZ DNA Methylation Kit (Zymo Research). Primers were designed using MethPrimer to target a region 1 kb downstream of the LIN28B transcriptional start site.
Transformation and Growth Assays
Soft agar assays were performed essentially as described previously with 50,000 cells seeded per well57. Colonies were stained with 0.005% crystal violet and counted after 3–4 weeks of growth.
Quantitative RT-PCR
quantitative RT-PCR was used for detection of mature miRNA species as described previously8. mRNA expression analysis was performed by quantitative PCR using SYBR green dye with fold-changes calculated by the ΔΔCt method or using LIN28B Taqman probe on patient samples.
Microarray Data Analysis
The following are Gene expression Omnibus accession numbers for publically available microarray datasets analyzed for LIN28 and LIN28B expression in this paper: GSE11024, GSE6222, GSE9843, GSE9891, GSE4170. Log2 transformed expression data were normalized to the average expression signal for normal tissue (where available), or row-normalized for the GSE9843 and GSE9891 datasets. Cutoff for over-expression was taken as a normalized expression value of 2 or greater unless stated otherwise. For microarray profiling of cancer cell lines, the samples run were from the Sanger Cell line collection as described at http://www.sanger.ac.uk/genetics/CGP/CellLines/ using an HT-HG_U133A chip, miRNAs were profiled using our previously described platform58. The HCC samples were stratified into LIN28B-high and -low groups based on a cut-off of 200U. LIN28B amplification status was determined in a panel of 1,103 cancer DNA samples – consisting of breast cancer (n = 242), chronic myelogenous leukemia (n = 6), lung non-small cell cancer (n=734), and hepatocellular carcinoma (n=121) – hybridized to the Affymetrix 250K StyI SNP Array (Beroukhim et al, Manuscript in preparation). Copy number estimates for LIN28B were normalized using a panel of population-matched controls and segmented using the Gain and Loss of DNA (GLAD) algorithm59.
Human tissue samples
The expression levels of let-7 species and LIN28B were assessed in 89 HCV-related hepatocellular carcinoma (HCC) samples including 77 clinically early HCC (25 pathologically early and 52 pathologically advanced cases) and 12 very advanced cases. All the samples were obtained from patients undergoing liver resection in the three hospitals of the HCC Genomic Consortium: Mount Sinai School of Medicine, NY (US), Hospital Clinic, Barcelona (Spain) and Istituto Nazionale dei Tumori, Milan (Italy). We obtained approval from the Institutional Review Board and patients were properly consented.
Quantitative PCR detection of LIN28 and LIN28B on CML samples
Total RNA was isolated from cell lines and patient samples (a mixture of both peripheral blood and bone marrow) using Trizol reagent (Invitrogen; Carlsbad, CA). cDNA was generated from 1mg of total RNA using Cloned AMV Reverse Transcriptase (Invitrogen). The cDNA synthesis was performed under the following thermocycler conditions: 25°C for 10 minutes, 42°C for 60 minutes, 99°C for 5 minutes and 4°C hold. Gene specific primers and probes were designed using Primer Express Version 3 (ABI; Foster City, CA). All of the QPCR primers and florescent probes were manufactured by Integrated DNA Technologies (Coralville, IA). Real-time quantitative PCR (QPCR) was performed on 100ng of synthesized cDNA in a 50ml reaction using the ABI 7900HT Real-Time PCR System under the following conditions: stage 1, 95°C for 10 minutes: and stage 2, 95°C for 15 seconds, 60°C for 1 minute for a total of 40 cycles.
Fluorescence in-situ hybridization (FISH)
FISH was performed using standard protocols on metaphase nuclei as previously described.1 The RP-770C15 and RP-809N15 BAC probes were obtained from the RPCI-11 human BAC library and directly labelled with SpectrumGreen and SpectrumRed, respectively, via a nick translation kit (Vysis), hybridized in a humidified chamber overnight at 37°C, and counterstained with DAPI. Detection was performed using a Zeiss Axioplan epifluorescence microscope equipped with a COHU-CCD camera and Quips software.
Supplementary Material
Acknowledgements
We thank Carla Kim for providing LKR cells and the MGH Gyn Tissue Repository for ovarian tissue samples. Thanks to Dieter Fink and Natalya Melnyk for technical assistance. This work was partially supported by grants from the U.S. National Institute of Diabetes and Digestive and Kidney Diseases (J.M.L; 1R01DK076986-01), the Samuel Waxman Cancer Research Foundation (J.M.L.), the Spanish National Health Institute (J.M.L; SAF-2007-61898). This work was supported by funds from the National Cancer Institute of Canada (NCIC) (P.S.). This work was supported by grants from the NIH, the NIH Director's Pioneer Award of the NIH Roadmap for Medical Research, Clinical Scientist Awards in Translational Research from the Burroughs Wellcome Fund and the Leukemia and Lymphoma Society (GQD), and the Howard Hughes Medical Institute (GQD).
1. Lu J, et al. MicroRNA expression profiles classify human cancers. Nature. 2005;435:834–838. [PubMed]
2. Thomson JM, et al. Extensive post-transcriptional regulation of microRNAs and its implications for cancer. Genes Dev. 2006;20:2202–2207. [PubMed]
3. Kumar MS, Lu J, Mercer KL, Golub TR, Jacks T. Impaired microRNA processing enhances cellular transformation and tumorigenesis. Nat.Genet. 2007;39:673–677. [PubMed]
4. Kumar MS, et al. Suppression of non-small cell lung tumor development by the let-7 microRNA family. Proc.Natl.Acad.Sci.U.S.A. 2008;105:3903–3908. [PubMed]
5. Heo I, et al. Lin28 mediates the terminal uridylation of let-7 precursor MicroRNA. Mol.Cell. 2008;32:276–284. [PubMed]
6. Newman MA, Thomson JM, Hammond SM. Lin-28 interaction with the Let-7 precursor loop mediates regulated microRNA processing. RNA. 2008;14:1539–1549. [PubMed]
7. Rybak A, et al. A feedback loop comprising lin-28 and let-7 controls pre-let-7 maturation during neural stem-cell commitment. Nat.Cell Biol. 2008;10:987–993. [PubMed]
8. Viswanathan SR, Daley GQ, Gregory RI. Selective blockade of microRNA processing by Lin28. Science. 2008;320:97–100. [PMC free article] [PubMed]
9. Inamura K, et al. let-7 microRNA expression is reduced in bronchioloalveolar carcinoma, a non-invasive carcinoma, and is not correlated with prognosis. Lung Cancer. 2007;58:392–396. [PubMed]
10. Takamizawa J, et al. Reduced Expression of the let-7 MicroRNAs in Human Lung Cancers in Association with Shortened Postoperative Survival. Cancer Res. 2004;64:3753–3756. [PubMed]
11. Gramantieri L, et al. Cyclin G1 Is a Target of miR-122a, a MicroRNA Frequently Down-regulated in Human Hepatocellular Carcinoma. Cancer Res. 2007;67:6092–6099. [PubMed]
12. Schultz J, Lorenz P, Gross G, Ibrahim S, Kunz M. MicroRNA let-7b targets important cell cycle molecules in malignant melanoma cells and interferes with anchorage-independent growth. Cell Res. 18:549–557. (0 AD) [PubMed]
13. Shell S, et al. Let-7 expression defines two differentiation stages of cancer. Proceedings of the National Academy of Sciences. 2007;104:11400–11405. [PubMed]
14. Esquela-Kerscher A, et al. The let-7 microRNA reduces tumor growth in mouse models of lung cancer. Cell Cycle. 2008;7:759–764. [PubMed]
15. Lee YS, Dutta A. The tumor suppressor microRNA let-7 represses the HMGA2 oncogene. Genes Dev. 2007;21:1025–1030. [PubMed]
16. Mayr C, Hemann MT, Bartel DP. Disrupting the Pairing Between let-7 and Hmga2 Enhances Oncogenic Transformation. Science. 2007;315:1576–1579. [PMC free article] [PubMed]
17. Zhang L, et al. Genomic and epigenetic alterations deregulate microRNA expression in human epithelial ovarian cancer. Proceedings of the National Academy of Sciences. 2008;105:7004–7009. [PubMed]
18. Nagayama K, et al. Homozygous deletion scanning of the lung cancer genome at a 100-kb resolution. Genes Chromosomes.Cancer. 2007;46:1000–1010. [PubMed]
19. Yamada H, et al. Detailed characterization of a homozygously deleted region corresponding to a candidate tumor suppressor locus at 21q11-21 in human lung cancer. Genes Chromosomes.Cancer. 2008;47:810–818. [PubMed]
20. Lu L, Katsaros D, Rigault de la Longrais I, Sochirca O, Yu H. Hypermethylation of let-7a-3 in Epithelial Ovarian Cancer Is Associated with Low Insulin-like Growth Factor-II Expression and Favorable Prognosis. Cancer Res. 2007;67 10117–10122. [PubMed]
21. Keath EJ, Caimi PG, Cole MD. Fibroblast lines expressing activated c-myc oncogenes are tumorigenic in nude mice and syngeneic animals. Cell. 1984;39:339–348. [PubMed]
22. Dangi-Garimella S, et al. Raf kinase inhibitory protein suppresses a metastasis signalling cascade involving LIN28 and let-7. Embo J. 2009;28:347–358. [PubMed]
23. Piskounova E, et al. Determinants of microRNA processing inhibition by the developmentally regulated RNA-binding protein Lin28. J.Biol.Chem. 2008;283 21310–21314. [PubMed]
24. Engelman A, Rosenberg N. bcr/abl and src but not myc and ras replace v-abl in lymphoid transformation. Mol.Cell Biol. 1990;10:4365–4369. [PMC free article] [PubMed]
25. Meyer B, Krisponeit D, Junghanss C, Escobar HM, Bullerdiek J. Quantitative expression analysis in peripheral blood of patients with chronic myeloid leukaemia: Correlation between HMGA2 expression and white blood cell count. Leukemia and Lymphoma. 2007;48:2008–2013. [PubMed]
26. Bruecher-Encke B, Griffin JD, Neel BG, Lorenz U. Role of the tyrosine phosphatase SHP-1 in K562 cell differentiation. Leukemia. 2001;15:1424–1432. [PubMed]
27. Chiang DY, et al. Focal gains of VEGFA and molecular classification of hepatocellular carcinoma. Cancer Res. 2008;68:6779–6788. [PMC free article] [PubMed]
28. Hoshida Y, et al. Gene expression in fixed tissues and outcome in hepatocellular carcinoma. N.Engl.J.Med. 2008;359:1995–2004. [PMC free article] [PubMed]
29. Korshunov A, Sycheva R, Golanov A, Pronin I. Gains at the 1p36 chromosomal region are associated with symptomatic leptomeningeal dissemination of supratentorial glioblastomas. Am J Clin.Pathol. 2007;127:585–590. [PubMed]
30. Dai Z, et al. A comprehensive search for DNA amplification in lung cancer identifies inhibitors of apoptosis cIAP1 and cIAP2 as candidate oncogenes. Hum.Mol.Genet. 2003;12:791–801. [PubMed]
31. Summersgill B, et al. Molecular cytogenetic analysis of adult testicular germ cell tumours and identification of regions of consensus copy number change. Br J Cancer. 1998;77:305–313. [PMC free article] [PubMed]
32. Chang TC, et al. Lin-28B transactivation is necessary for Myc-mediated let-7 repression and proliferation. Proc Natl Acad Sci U.S.A. 2009;106:3384–3389. [PubMed]
33. Anglesio MS, et al. Differential expression of a novel ankyrin containing E3 ubiquitin-protein ligase, Hace1, in sporadic Wilms' tumor versus normal kidney. Hum.Mol.Genet. 2004;13:2061–2074. [PubMed]
34. Bruce CK, Howard P, Nowak NJ, Hoban PR. Molecular analysis of region t(5;6)(q21;q21) in Wilms tumor. Cancer Genetics and Cytogenetics. 2003;141:106–113. [PubMed]
35. Fernandez CV, Lestou VS, Wildish J, Lee CLY, Sorensen PHB. Detection of a novel t(6;15)(q21;q21) in a pediatric Wilms tumor. Cancer Genetics and Cytogenetics. 2001;129:165–167. [PubMed]
36. Hoban PR, et al. Physical localisation of the breakpoints of a constitutional translocation t(5;6)(q21;q21) in a child with bilateral Wilms' tumour. J Med Genet. 1997;34:343–345. [PMC free article] [PubMed]
37. Bruce CK, Howard P, Nowak NJ, Hoban PR. Molecular analysis of region t(5;6)(q21;q21) in Wilms tumor. Cancer Genetics and Cytogenetics. 2003;141:106–113. [PubMed]
38. Zhang L, et al. The E3 ligase HACE1 is a critical chromosome 6q21 tumor suppressor involved in multiple cancers. Nat Med. 2007;13:1060–1069. [PubMed]
39. Zhang L, et al. The E3 ligase HACE1 is a critical chromosome 6q21 tumor suppressor involved in multiple cancers. Nat Med. 2007;13:1060–1069. [PubMed]
40. Richards M, Tan SP, Tan JH, Chan WK, Bongso A. The Transcriptome Profile of Human Embryonic Stem Cells as Defined by SAGE. Stem Cells. 2004;22:51–64. [PubMed]
41. Eckfeldt CE, et al. Functional Analysis of Human Hematopoietic Stem Cell Gene Expression Using Zebrafish. PLoS Biology. 2005;3:e254. [PMC free article] [PubMed]
42. Yang DH, Moss EG. Temporally regulated expression of Lin-28 in diverse tissues of the developing mouse. Gene Expression Patterns. 2003;3:719–726. [PubMed]
43. Bell DA. Origins and molecular pathology of ovarian cancer. Mod Pathol. 18:S19–S32. (0 AD). [PubMed]
44. Lee JS, et al. A novel prognostic subtype of human hepatocellular carcinoma derived from hepatic progenitor cells. Nat Med. 2006;12:410–416. [PubMed]
45. Yu J, et al. Induced pluripotent stem cell lines derived from human somatic cells. Science. 2007;318:1917–1920. [PubMed]
46. Lessard J, Sauvageau G. Bmi-1 determines the proliferative capacity of normal and leukaemic stem cells. Nature. 2003;423:255–260. [PubMed]
47. Gidekel S, Pizov G, Bergman Y, Pikarsky E. Oct-3/4 is a dose-dependent oncogenic fate determinant. Cancer Cell. 2003;4:361–370. [PubMed]
48. Hochedlinger K, Yamada Y, Beard C, Jaenisch R. Ectopic expression of Oct-4 blocks progenitor-cell differentiation and causes dysplasia in epithelial tissues. Cell. 2005;121:465–477. [PubMed]
49. Ben Porath I, et al. An embryonic stem cell-like gene expression signature in poorly differentiated aggressive human tumors. Nat Genet. 2008;40:499–507. [PMC free article] [PubMed]
50. Hartwell KA, et al. The Spemann organizer gene, Goosecoid, promotes tumor metastasis. Proc Natl Acad Sci U.S.A. 2006;103 18969–18974. [PubMed]
51. Yang J, et al. Twist, a master regulator of morphogenesis, plays an essential role in tumor metastasis. Cell. 2004;117:927–939. [PubMed]
52. Yu F, et al. let-7 regulates self renewal and tumorigenicity of breast cancer cells. Cell. 2007;131:1109–1123. [PubMed]
53. Llovet JM, Burroughs A, Bruix J. Hepatocellular carcinoma. Lancet. 2003;362:1907–1917. [PubMed]
54. Azam M, Seeliger MA, Gray NS, Kuriyan J, Daley GQ. Activation of tyrosine kinases by mutation of the gatekeeper threonine. Nat.Struct.Mol.Biol. 2008 [PMC free article] [PubMed]
55. Johnson L, et al. Somatic activation of the K-ras oncogene causes early onset lung cancer in mice. Nature. 2001;410:1111–1116. [PubMed]
56. Park IH, et al. Reprogramming of human somatic cells to pluripotency with defined factors. Nature. 2008;451:141–146. [PubMed]
57. Lee JC, et al. Epidermal growth factor receptor activation in glioblastoma through novel missense mutations in the extracellular domain. PLoS.Med. 2006;3:e485. [PMC free article] [PubMed]
58. Lu J, et al. MicroRNA expression profiles classify human cancers. Nature. 2005;435:834–838. [PubMed]
59. Hupe P, Stransky N, Thiery JP, Radvanyi F, Barillot E. Analysis of array CGH data: from signal ratio to gain and loss of DNA regions. Bioinformatics. 2004;20:3413–3422. [PubMed]