|Home | About | Journals | Submit | Contact Us | Français|
Phosphorus-containing pseudopeptides, racemic at the C-terminal α-carbon, are potent mechanism-based inhibitors of folylpolyglutamate synthetase (FPGS). They are mimics of the tetrahedral intermediate postulated to form during FPGS-catalyzed biosynthesis of poly(γ-L-glutamates). In the present paper, the FPGS inhibitory activity of each diastereomer coupled to three heterocycles is reported. The high Rf pseudopeptide containing the 5,10-dideazatetrahydropteroyl (DDAH4Pte) heterocycle is most potent (Kis = 1.7 nM). While the heterocyclic portion affects absolute FPGS inhibitory potency, the high Rf species is more potent in each pair containing the same heterocycle. This species presumably has the same stereochemistry as the natural folate polyglutamate, i.e., (L-Glu-γ-L-Glu). Unexpectedly, the low Rf (presumed L-Glu-γ-D-Glu) species are only slightly less potent (<30-fold) less potent than their diastereomers. Further study of this phenomenon comparing L-Glu-γ-L-Glu and L-Glu-γ-D-Glu dipeptide-containing FPGS substrates shows that <1% contamination of commercial D-Glu precursors by L-Glu may give misleading information if L-Glu-γ-L-Glu substrates have low Km values.
The intracellular folate pool consists of a family of derivatives of folic acid that differ in reduction state, presence and nature of one-carbon substituents, and occurrence of poly(γ-glutamyl) chains ligated to the glutamate (Glu)1 intrinsic to the folic acid structure . Folates, in their fully reduced 5,6,7,8-tetrahydro state, participate in a variety of anabolic and catabolic one-carbon transfer reactions, including those in thymidylate synthesis, de novo purine synthesis, and synthesis of serine, glycine, and methionine . Poly(γ-glutamylation) of folates with up to seven additional Glu residues in mammalian cells serves two major functions . Polyglutamylation serves to retain folates inside cells since only monoglutamates are substrates for folate efflux systems and the high negative charge associated with polyglutamylation at physiological pH precludes diffusion through the membrane. In addition, polyglutamates serve as the preferred substrates (higher Vmax/Km) for virtually all folate-dependent enzymes.
Polyglutamylation of folates is an essential process because mutational deletion of folylpolyglutamate synthetase (FPGS), the sole enzyme responsible for their synthesis, is lethal unless all the end-products of folate metabolism are supplied (i.e., thymidine, purines, serine, glycine, methionine, etc.) [4, 5]. This essential requirement for polyglutamylation has led numerous investigators to propose FPGS as a target in cancer chemotherapy [6, 7]. Our laboratories have investigated several different classes of potential inhibitors in an effort to identify potent and specific FPGS inhibitors [7–9]. Recently, we described phosphorus-containing pseudopeptides in which the tetrahedral PV species (as a phosphonate (Figure 1, ,2a)2a)  or a phosphinate (Figure 1, ,2b)2b) [11, 12]) serves as a mimic of the transient tetrahedral intermediate (Figure 1, 1) derived from the γ-glutamyl phosphate reaction intermediate . Within this phosphorus-containing class, phosphinate-based inhibitors are clearly most potent . The phosphinates have another advantage in that there is precedent for further processing of the inhibitor via an ATP-dependent, enzyme-catalyzed reaction to form the phosphorylated phosphinate, 3 [14–18], with significant enhancement of inhibitory activity.
The initial proof-of-concept analogs were synthesized as mixtures of diastereomers , because of the lack of synthetic methodology for the stereoselective synthesis of complex phosphinate pseudopeptides. In addition, earlier analogs were all prepared with a single folate like heterocycle (4-amino-4-deoxy-10-methylpteroate; AMPte) common to methotrexate (MTX) to allow ready comparison both with earlier inhibitor classes and between those containing different oxidation states of phosphorus. It is known, however, that changing the heterocycle within one class of FPGS inhibitor can lead to greater potency and even increased specificity . Therefore, we have prepared individual diastereomers of the phosphinate-containing dipeptide mimics and each diastereomer has been coupled to three different heterocycles, including 4-amino-4-deoxy-10-methylpteroate (4) , pteroate (5), and 5,10-dideazatetrahydropteroate (6) (Figure 2). Inhibitory potency of each diastereomer and a structure-activity relationship for the heterocycles has been determined and the results are reported herein.
In addition to the phosphinates designed to mimic the tetrahedral intermediate formed during FPGS-catalyzed ligation of the first glutamate, phosphinate mimics of intermediates formed during subsequent ligations were also of interest. Thus, the synthesis of 7 (Figure 2), a pteroyl derivative that incorporates elements to mimic the intermediate formed during ligation of the second glutamate is described (Supplementary Material). The inhibitory properties of this analog are also reported.
Common chemicals were reagent grade or higher. MTX was a generous gift of Immunex (Amgen; Seattle, WA). Aminopterin (AMT) was from Sigma Chemical Co. (St. Louis, Mo). The phosphorus-containing diastereomeric pseudo-dipeptide and -tripeptide (phosphinate) precursors were prepared as described by Bartley and Coward . The required pteroyl azide , 4-amino-4-deoxy-10-methylpteroyl (MTX) azide , and 5,10-dideazatetrahydropteroyl (DDATHF) azide  were prepared as described. Coupling of the phosphinate precursor to the appropriate azide and subsequent work up was carried out similarly to that described for the MTX azide . Details of the synthetic procedure and characterization of intermediates and products (5–7) are presented in Supplementary Material associated with this article available in the online version, at doi: ############. Suitably blocked –L-Glu-γ-L-Glu and –L-Glu-γ-D-Glu dipeptides were synthesized as described previously for oligo-γ-glutamates consisting of L-Glu  and coupled to the various azides (above) to afford 8–10 as described previously  and in the Supplementary Material. Analog solutions for all experiments were standardized using extinction coefficients from the literature. It was assumed that the phosphinates had extinction coefficients identical to the heterocycle containing Glu. Since the spectra of the phosphinates were identical or nearly identical to those of the Glu-containing species, this assumption is warranted.
Recombinant human cytosolic FPGS was expressed in Sf9 cells and purified as described previously . FPGS substrate activity of analogs was determined as described . Time linearity was verified at low and high substrate concentrations in all assays. Because of the low Km for DDATHF, the concentration of [3H]Glu in assays was decreased to 2 mM (from 4 mM) and the DE-52 column wash solution was adjusted to 130 mM NaCl (from 110 mM NaCl); this concentration of NaCl was verified to allow quantitative retention of DDATHF and thus its polyglutamates would also be retained. Inhibitory potency (IC50) of analogs against rhcFPGS was determined as described . Inhibition constants Kii and Kis  for rhcFPGS were determined  using MTX as the variable substrate (30–1000 μM) and two levels of inhibitor yielding ≈25% and ≈50% inhibition, respectively; these were compared to an identical control lacking inhibitor. In some experiments, DDATHF was used as the variable substrate (1–25 μM) to verify that the class of variable substrate did not affect the potency and inhibitory mechanism. Pure carboxypeptidase G2 (CPG2) from Pseudomonas sp. strain RS-216 was a gift of the late Dr. Roger Sherwood (Microbial Technology Laboratory, PHLS Centre for Applied Microbiology and Research, Porton Down, England) . Cloned soluble recombinant glutamate carboxypeptidase II (GCP II), obtained from Dr. Takashi Tsukamoto (Guilford Pharmaceuticals) was assayed as described .
Semi-preparative HPLC purification of the phosphinate pseudopeptides, 4–7 and the L-Glu-γ-D-Glu derivatives, 8–10, employed an HPLC system (Rainin, Woburn MA) that consisted of two Rabbit Model HPX pumps fitted with 50 mL/min pump heads, a dynamic mixer, and a single wavelength UV detector. All separations utilized a Varian Dynamax 21.4 × 250 mm, Microsorb 60–8, C18 column with a flow rate of 21 mL/min. Purification conditions were as follows: Eluent A – 20 mM ammonium acetate, pH = 6.5, in ddH2O; Eluent B – MeOH; Gradient – 0 min, 5% B; 10 min, 5% B; 20 min, 50% B; 25 min 50%B. Details on the purification of each compound can be found in the Supplementary Material (doi: ############).
Further semi-preparative HPLC purification of Pte-L-Glu-γ-D-Glu was performed on a 4.6 × 250 mm analytical C18 column (5 μ, Rainin Microsorb, Varian, Walnut Creek, CA) using 0.1 M Na-acetate, pH 5.5 containing 4% acetonitrile (ACN) isocratically at 1 ml/min for 30 min and injecting no more than 2.4 μmol/400 μL injection using a 500 μL loop. Individual 1 min (1 ml) fractions were collected and each fraction was subjected to analytical HPLC as described above (15 min run time) to determine the level of putative Pte-L-Glu-γ-L-Glu contamination. Highly purified fractions of Pte-L-Glu-γ-D-Glu from multiple runs were pooled, exchanged into ammonium bicarbonate buffer by DEAE-cellulose (DE-23; Whatman, Inc., Florham Park, NJ) chromatography, lyophilized to remove salts, and re-analyzed. The original synthetic Pte-L-Glu-γ-D-Glu contained ≈0.7% of putative Pte-L-Glu-γ-L-Glu, while the final purified material contained <0.01%. Remaining fractions enriched for the putative Pte-L-Glu-γ-L-Glu contaminant were pooled separately, processed as above, and also analyzed by HPLC; the final material contained ≈2.1% of the contaminant. Analysis of GCP II hydrolysis products used a modified system employing 0.1 M Na-acetate, pH 5.5 containing 3% ACN isocratically for 15 min and then a 15 min linear gradient to 13% ACN to separate the two diastereomers while still allowing detection of the PteGlu hydrolysis product; typical Rt were L,L (16.9 min), L,D (19.4 min), and PteGlu (27.7 min).
Four separate diastereomeric pairs (low Rf and high Rf) of the phosphinic acid-containing pseudopeptide were prepared, each pair of which contained a different heterocycle and/or chain length (Table 1). The pairs based on MTX (AMPte, 4) were of immediate interest because this heterocycle has been used as a standard in earlier FPGS inhibitor design efforts. We previously showed that the IC50 for the mixture containing all four diastereomers was 8 ± 1 nM ; in the current studies, the value obtained was 13.6 ± 1.3 nM, indicating good agreement. Comparing the two diastereomers containing this heterocycle, the high Rf species was about 13-fold more potent than the low Rf species (IC50, Table 1). Current knowledge of FPGS stereospecificity thus suggests that the high Rf species is analogous to the natural L-Glu-γ-L-Glu configuration. Further characterization revealed that the high Rf species was a competitive inhibitor of rhcFPGS (KisKii) with a low nM Kis. A replot of Km/Vmax vs. [inhibitor] was linear and thus inhibition was linear competitive . We did not observe time-dependence in inhibitory potency of any phosphinate isomer when we compared residual activity at t0.5 and t, similar to our previous studies with mixtures of diastereomers . However, because of the lengthy (90–120 min) reaction time course required to observe FPGS activity, we cannot preclude time-dependent events that occur in the first few minutes of the reaction. It is well known that FPGS exhibits significant ATPase activity [25–27]. For this reason, it was not possible to demonstrate ATP-dependent, FPGS-catalyzed formation of a phosphorylated phosphinate, 3 (Figure 1) .
Substitution of other heterocycles (pteroate (Pte) or 5,10-dideazatetrahydropteroate (DDAH4Pte)) altered the absolute potency, but did not change the relationship between potency of low and high Rf species (IC50, Table 1). In each case, the high Rf species was significantly more potent than the low Rf species. The most potent inhibitor was the high Rf species containing the DDAH4Pte heterocycle. The high Rf diastereomer was kinetically characterized as an inhibitor with both MTX and DDAH4PteGlu (DDATHF) as the variable substrate. Similar inhibitor constants were obtained as expected if a single kinetic site is involved (Table 1) and the inhibition was linear competitive regardless of the substrate used. The high potency of the DDAH4Pte-containing pseudopeptide mimic of the tetrahedral intermediate formed during the biosynthesis of the Glu-γ-Glu product was anticipated based on the low Km value (0.65 μM; ) of the corresponding Glu-γ-Glu dipeptide as an FPGS substrate. However, the relative potencies of the other high Rf dipeptide species did not parallel the Km values of their corresponding Glu-γ-Glu dipeptides as FPGS substrates (PteGlu2, Km = 16 μM  and AMPteGlu2, Km = 63 μM ). The following values of Km/Kis were determined for each L-Glu-γ-L-Glu dipeptide substrate and its corresponding pseudopeptide inhibitor: AMPte, 27.4 × 103; Pte, 3.4 × 103, and DDAH4Pte, 0.7 × 103. This is in contrast to an earlier series of substantially weaker FPGS inhibitors in which the intrinsic Glu was replaced by ornithine , where their relative potencies mirrored the efficiencies of the Glu-containing parents as FPGS substrates. In the current research, values of Ki reflect events, probably including protein dynamics, associated with binding of an analog of an unstable reaction intermediate. In contrast, values of Km reflect ground-state events during the binding and turnover of substrates. Presumably, the ornithine derivatives also bind as ground-state analogs. In related research on glutathionyl spermidine synthetase/amidase (EC 22.214.171.124/126.96.36.199), a bifunctional protein that encodes both an ATP-dependent ligase and a cysteine amidase, binding of a similar phosphinate-containing “tetrahedral mimic” to the synthetase domain has a marked effect on both the catalytic activity  and structure  of the amidase domain. This suggests that the primary source of binding energy for these analogs comes from the phosphinate-containing pseudodipeptide, and that these mimics of unstable intermediates formed during catalysis are able to recruit distal domains to the ligand-protein interactions.
The lower potency of the Pte-(γ-Glu)3 mimic 7 with the phosphinate in the terminal position relative to the Pte-(γ-Glu)2 mimic was unanticipated in the initial design since PteGlu2 (Km = 16 μM ) has a Km value similar to that of PteGlu3 (Km = 20 μM ) which suggests the possibility that their affinities (Kd) could also be similar. However, subsequent to the synthesis of 7, detailed kinetic studies with DDATHF provided evidence for the processive ligation of multiple glutamates at a low but saturating (~10 Km) folate substrate concentration . Thus, the first γ-ligation is fast followed by slower steady-state elongation to higher chain length products. It would be expected that the longer chain oligoglutamates would not have access to the FPGS active site during processive ligation of multiple glutamates. This restrictive access of higher chain length folate substrates or analogs to the FPGS active site is consistent with the markedly decreased inhibitory activity of the Glu-γ-Glu-γ-Glu analog, 7. However, only one member of this class was prepared, and further work will be required to determine if this is a general feature of pseudotripeptide mimics, is specific for the Pte heterocycle, or is a function of the position of the phosphinate tetrahedral mimic.
These studies demonstrate that very potent mechanism-based inhibitors of purified human FPGS employing a variety of heterocycles can be designed and synthesized. Because phosphinate-containing pseudodipeptide inhibitors such as 4–8 apparently do not enter human cells , the next task is to transform these inhibitors into cell-permeable drugs. In this regard, at least two approaches can be envisioned. The first is to synthesize prodrugs that will allow these hydrophilic moieties to enter cells for subsequent activation. An initial attempt in this regard utilized readily hydrolyzable pivaloyloxymethyl (POM) esters that have been used previously to deliver several hydrophilic drugs . Unfortunately, previously unrecognized relatively rapid (t0.5 = 30 min) hydrolysis of the esters in medium or solvent was documented. However, the product of this rapid intramolecular reaction is not the parent drug . Further effort will be required to generate a suitable prodrug. The second approach to developing cell-permeable FPGS inhibitors involves determining the minimum structure of the phosphinate-containing pseudodipeptide that is required for potent inhibition. This approach may lead to new inhibitors that possess an intrinsically higher ability to enter cells either by diffusion or by mediated processes. Investigations of both approaches are continuing.
Current evidence suggests that FPGS is highly stereospecific for L-Glu (or close L-Glu structural analogs); this evidence is direct for the position occupied by the intrinsic Glu (position 1; [25, 32, 33]), but indirect for the first γ-Glu (position 2; ). Thus, the data of Table 1 were unexpected because the low Rf species, which presumably mimic the unnatural L-Glu-γ-D-Glu configurations, remain highly potent FPGS inhibitors. The high affinity of this unnatural configuration suggested that the substrate activity of pteroyl-L-Glu-γ-D-Glu species should be directly evaluated. Accordingly, the L-Glu-γ-L-Glu and L-Glu-γ-D-Glu dipeptides conjugated to the Pte, AMPte, and DDAH4Pte heterocycles were synthesized (Supplementary Material; doi: ############) for characterization as FPGS substrates. Because of limitations in the supply of purified FPGS, only single substrate concentrations were evaluated, which were equivalent to 4 – 10 Km for the respective L-Glu-γ-L-Glu conjugates of each heterocycle [10, 21, 28]. The L-Glu-γ-L-Glu conjugates were confirmed as active FPGS substrate (Table 2). In contrast, the L-Glu-γ-D-Glu conjugates of Pte and AMPte showed no significant activity (Table 2). However, the Km values of the corresponding L-Glu-γ-L-Glu conjugates are relatively high (vide supra), and with the limited amount of compound and FPGS available, we cannot preclude low activity for the L-Glu-γ-D-Glu conjugates that might be apparent only at very high substrate concentrations. Consistent with this hypothesis, DDAH4Pte-L-Glu-γ-D-Glu showed activity equivalent to DDAH4Pte-L-Glu-γ-L-Glu when both were present at 100 μM (200 Km) (Table 2). A full concentration-dependence curve employing similarly pure human FPGS (Tsai and Coward, unpublished results) showed similar results and yielded a Km value for DDAH4Pte-L-Glu-γ-D-Glu of ≈9 μM, which was 19-fold higher than that of DDAH4Pte-L-Glu-γ-L-Glu (Km, 0.5 μM) in the same experiment; in addition, the Vmax of DDAH4Pte-L-Glu-γ-D-Glu was ≈66% that of DDAH4Pte-L-Glu-γ-L-Glu, thus leading to Vmax/Km values of 3.5 × 10−3s−1 and 1.1 × 10−4s−1 for the L-Glu-γ-L-Glu and L-Glu-γ-D-Glu isopeptides, respectively.
Because the Km of the DDAH4Pte-containing L,L species is very low relative to the L,D species, the possibility that DDAH4Pte-L-Glu-γ-D-Glu contained low levels of its L,L diastereomer was considered. Consistent with this suggestion was the finding (Table 2) that the product distribution at a high concentration of DDAH4Pte-L-Glu-γ-D-Glu was similar to that observed at the low concentration of DDAH4Pte-L-Glu-γ-L-Glu and contained multiple products, including those having long chain length. We previously noted [21, 25, 30] the inverse concentration-dependence of product length for FPGS such that low concentrations of effective substrates tend to promote synthesis of longer products, while higher concentrations tend to produce only one product containing a single additional γ-Glu. Thus a nonsubstrate containing a low concentration contaminant that is an efficient FPGS substrate would tend to produce multiple products including those containing long chain lengths. The reversed-phase HPLC system employed to determine general purity did not resolve DDAH4Pte-L-Glu-γ-D-Glu from DDAH4Pte-L-Glu-γ-L-Glu (or AMPte-L-Glu-γ-D-Glu from AMPA-L-Glu-γ-L-Glu) despite extensive efforts to develop modified methods to effect these separations. However, Pte-L-Glu-γ-L-Glu (Rt 18.8 min) and Pte-L-Glu-γ-D-Glu (Rt 22.4 min) were nearly baseline resolved. Because the same dipeptide precursors are conjugated to each heterocycle, if the presence of an L,L diastereomer contaminant can be demonstrated in any L,D diastereomer, it suggests that it is present in all. Accordingly, we focused on determining stereochemical purity of Pte-L-Glu-γ-D-Glu.
Initial HPLC analysis showed that a small (0.7% by area) peak eluting at the Rt of Pte-L-Glu-γ-L-Glu was present in synthetic Pte-L-Glu-γ-D-Glu. Co-injection of Pte-L-Glu-γ-D-Glu with a trace of Pte-L-Glu-γ-L-Glu demonstrated the co-elution of the contaminant peak with Pte-L-Glu-γ-L-Glu. To corroborate this finding, we explored sensitivity of these peaks to enzymatic hydrolysis. Initially we attempted to use carboxypeptidase G2 (CPG2) to hydrolyze L,L-containing dipeptides because the closely related enzyme CPG1 was reported to catalyze hydrolysis of pteroyl-(γ-L-Glu)3 . However, under conditions where a pteroyl-L-Glu itself was quantitatively hydrolyzed in 30 min (half-time, ≈6 min), CPG2 failed to hydrolyze the corresponding pteroyl-L-Glu-γ-L-Glu (<0.8% hydrolysis in 60 min at 10× CPG2). Consequently, we employed a cloned, purified soluble glutamate carboxypeptidase II (GCP II; also known as PSMA or NAALAdase; ), which possesses γ-L-glutamyl hydrolase activity [36, 37], but which does not hydrolyze AMPte-L-Glu-γ-D-Glu . To increase sensitivity, a side-fraction obtained during HPLC purification of Pte-L-Glu-γ-D-Glu (Methods) containing ≈2% of the putative L,L contaminant was employed. HPLC analysis after GCP II hydrolysis of this material showed that the main Pte-L-Glu-γ-D-Glu peak was not hydrolyzed; the putative Pte-L-Glu-γ-L-Glu contaminant, however, was reduced >96% while producing a concomitant increase in a peak with the retention time of the expected hydrolysis product PteGlu. Similar results were obtained following PSMA treatment of HPLC-purified Pte-L-Glu-γ-D-Glu that contained <0.01% of the putative L,L material. These data are consistent with the contaminant being the L,L-containing diastereomer. Since the same dipeptide was used to synthesize DDAH4Pte-L-Glu-γ-D-Glu, the data further suggest that this diastereomer is contaminated with as much as 0.7% DDAH4Pte-L-Glu-γ-L-Glu and this level could produce the FPGS substrate activity observed (Table 2) because of the low Km of the L,L diastereomer. Our results show that caution must be used when interpreting the results of studies of the stereospecificity of FPGS, especially when one isomer has an especially low Km value. The occurrence of contaminating diastereomers of antifolate γ-dipeptides has been noted previously , but the biochemical consequences of these contaminants, especially with respect to FPGS activity, were not reported. Returning to the phosphinate-containing pseudopeptides, the two stereocenters of the final products were derived from, respectively, commercial L-glutamic acid to yield the 2S (L-) configuration at the N-terminal glutamate and a stereorandom synthesis of the C-terminal moiety. Purification of the diastereomeric mixture provided the “high Rf” and “low Rf” products that have been evaluated as reported in this paper. Based on the route of synthesis, contamination of pseudopeptides 4–6 with other stereoisomers is highly unlikely.
The authors wish to acknowledge Mr. Sanyo Tsai for assisting in the synthesis of the blocked L-Glu-γ-L-Glu and L-Glu-γ-D-Glu dipeptides and their conjugation to the various heterocycles and also for the initial kinetic analysis of the DDAH4Pte diastereomers as FPGS substrates. The authors thank Dr. Takashi Tsukamoto of Guilford Pharmaceuticals for his generous gift of purified recombinant human GCP II. This work was supported by grants CA43500 (JJM), Cancer Center Support Grant CA16056 to Roswell Park Cancer Institute, and CA28097 (JKC). D.M.B. was a trainee in the Pharmaceutical Sciences Training Program supported by grant GM007767 from National Institutes of General Medical Sciences (NIGMS). J.W.T. was a trainee of the Michigan Chemistry-Biology Interface Training Program, supported in part by grant GM008597 from NIGMS. J.W.T. was the recipient of a Fred W. Lyons Fellowship from the College of Pharmacy, University of Michigan, and a fellowship from the American Foundation for Pharmaceutical Education. The contents are solely the responsibility of the authors and do not necessarily represent the official views of NCI or NIGMS.
1Abbreviations used: ACN, acetonitrile; AMPte, 4-amino-4-deoxy-10-methylpteroate; AMT, aminopterin (4-amino-4-deoxy-pteroylglutamic acid); CPG2, carboxypeptidase G2 (EC 188.8.131.52); DDAH4Pte, (6R)-5,10-dideazatetrahydropteroate; DDAH4PteGlu or DDATHF, (6R)-5,10-dideazatetrahydropteroylglutamate; FPGS, folylpolyglutamate synthetase (EC 184.108.40.206); Glu, glutamic acid; GCP II, glutamate carboxypeptidase II (EC 220.127.116.11); MTX, methotrexate (4-amino-4-deoxy-10-methylpteroylglutamic acid); Pte, pteroate.
Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.