Search tips
Search criteria 


Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
J Mol Biol. Author manuscript; available in PMC 2009 October 5.
Published in final edited form as:
PMCID: PMC2757166

Remodeling of the Human Papillomavirus Type 11 Replication Origin into Discrete Nucleoprotein Particles and Looped Structures by the E2 Protein


The human papillomavirus (HPV) origin (ori) of DNA replication shares a common theme with many DNA control elements in having multiple binding sites for one or more proteins spaced over several hundred base pairs. The HPV type-11 ori spans 103 bp and contains three palindromic binding sites (E2BS-2, E2BS-3, and E2BS-4) for the dimeric E2 origin binding protein. These sites are separated by 64 bp and 3 bp. E2BS-1 is located 288 bp upstream of E2BS-2 and is not required for efficient transient or cell-free replication. In this study, electron microscopy was used to visualize complexes of HPV-11 ori DNA bound by purified E2 protein. DNA containing only E2BS-2 showed a single E2 dimer bound. DNA containing E2BS-3 and E2BS-4 showed two side-by-side E2 dimers, while DNA containing E2BS-2, E2BS-3, and E2BS-4 exhibited a large disk/ring-shaped protein particle bound indicating that the DNA had been remodeled into a discrete complex, likely containing an E2 hexamer. With all four binding sites present, up to 27% of the DNA molecules were arranged into loops by E2, the majority of which spanned E2BS-1 and one of the other three sites. Studies of the dependence of looping on salt, ATP, and DTT using full length E2 and an E2 protein containing only the carboxyl-terminal DNA binding and protein dimerization domain suggest that looping is dependent on the N terminal domain as well as factors which may affect the manner in which E2 scans DNA for binding sites. The role of these structures in the modeling and regulation of the HPV-11 ori is discussed.

Keywords: HPV, E2, EM, DNA binding, DNA looping


A common structural theme of eukaryotic replication and transcriptional promoters is the presence of arrays of binding sites for DNA binding proteins. These sites may be close together or separated by up to hundreds of base pairs, suggesting that the DNA provides a scaffold upon which complex three dimensional nucleoprotein structures are sculpted. There are many ways in which this can occur, including the formation of DNA loops and condensed nucleoprotein particles. The binding of human papillomavirus (HPV) type 11 E2 protein to the homologous ori offers an excellent model for understanding how such DNA-protein remodeling may occur and, in turn, regulate DNA function.

HPVs are a family of small DNA viruses which infect human squamous epithelia at mucosal or cutaneous sites, causing benign hyperproliferative, warty lesions. HPV has a double-stranded (ds) circular DNA genome of approximately 7.9 kb and replicates as multi-copy nuclear plasmids. Viral DNA replication requires the viral ori and the cellular DNA replication machinery. The virus contributes two replication factors, the ori-binding protein E2 and the replicative DNA helicase E1.14 The papillomaviral protein E1 is required throughout initiation and elongation5, as it is an ATP-dependent helicase, equivalent to the cellular replicative DNA helicase (the minichromosome maintenance complex). In contrast, the ori-binding E2 protein is only essential for the assembly of the pre-initiation complex and is absent during the elongation phase of papillomavirus DNA replication.5 In addition, E2 is also responsible for equitable viral genome segregation into daughter cells.611

The HPV-11 ori spans 103 bp and lies at the 3′ end of the upstream regulatory region (URR), a 700 bp long non-coding region which also controls transcription from the adjacent E6 promoter. The ori is comprised of three E2 binding sites (E2BSs) flanking an array of E1 BSs where E1 assembles into a di-hexameric helicase. The three E2BSs are separated by 64 and 3 bp from one another. E2BS-2 lies immediately upstream of the E1BS whereas the E2BS-3 and E2BS-4 are located just downstream. E2BS-4 is situated 3 bp upstream of the TATA sequence of the E6 promoter. By binding to the overlapping ori/promoter elements, E2 also functions as a transcription factor and modulates the activity of this promoter. The E1 protein is recruited to the ori by the E2 protein.12,13 Studies of HPV-11 E1 and E2 binding to the HPV ori using electron microscopy (EM) have shown that the cellular heat shock/chaperone proteins hsp40 and hsp70 facilitate the formation of the E1 di-hexamer at the ori, with the release of E2.14,15 In addition, there is another E2 binding site, E2BS-1, which lies 288 bp upstream of E2BS-2 and is not required for efficient transient replication in transfected cells or for cell-free replication.3,4,16 This sequence arrangement in the HPV-11 ori is highly conserved in many HPV genotypes.

The E2 proteins vary in size among the papillomavirus types including the bovine papillomavirus (BPV), ranging from 42–48 kDa, but their functions are highly conserved. The amino terminal half of the protein is the trans-acting domain which interacts with the E1 helicase, recruiting it to the ori, and with transcription factors.12,13,17 A central hinge region is poorly conserved in length and in sequence. However, for HPV-11 E2 and perhaps additional mucosotropic HPV genotypes, this region contains a nuclear localization sequence and also associates with the nuclear matrix.18 The carboxyl-terminal domain promotes E2 protein dimerization, and the dimer then binds in the major groove of the DNA at a 12-base pair palindromic sequence (5′-ACCGN4CGGT-3′).19 All papilloma virus E2 proteins recognize this 12 bp sequence although the 4 base pair spacer may vary among viruses.

The BPV ori/promoter contains a cluster of 12 E2 binding sites, and Knight et al.20 showed that BPV E2 binds DNA as a dimer and also forms larger protein particles when two E2 binding sites are spaced close together. Using EM, loops were occasionally noted when the binding sites were artificially spaced by a larger distance. This study also implicated the N terminal domain of BPV E2 in the loop formation. In an X-ray crystallographic study21, E2N dimers were observed with HPV-16 E2, but not with HPV-11 E222 nor with HPV-18 E2 truncated of residues 1–65.21 These studies suggested that an EM examination of the binding of HPV E2 to the natural HPV ori might reveal multiple levels of protein-mediated folding and remodeling of the DNA central to the control of HPV replication and transcription.

In this work we have carried out such a study using purified functional HPV-E2 protein and HPV-11 ori-spanning fragments containing from one to all four of the E2 binding sites. HPV-11 was selected for this study, as the structures and functions of the E1 and E2 proteins of this virus have been examined intensively, including collaborative EM work between the two laboratories involved in this present work. The 4 binding sites provide ample opportunity for remodeling events in the ori but without the complexity of the much larger number of E2 sites found in the BPV genome. We found that the cluster of three closely spaced sites generated a trimer of dimers arranged into a disk/ring-shaped hexamer. Moreover, when the DNA fragment contained all four binding sites, frequent DNA loops were observed between the distal E2BS-1 and one of the cluster of three adjacent sites some 288 bp downstream. E2C, which contains only the protein dimerization and DNA binding domain, abolished looping, indicating the amino terminal portion of the protein is necessary for DNA looping. On the basis of these structures, we discuss the implications on the assembly and regulation of functional replication complexes.


E2 and E2C proteins bind to E2BS in EMSA

The functionality of the proteins used in this study was tested by electrophoretic mobility shift assays (EMSA) using probes containing E2BS-3 and E2BS-4. As shown in Fig. 1, a slow migrating band was observed when either full length E2 or E2C containing only the carboxyl terminal 83 amino acids (a.a.) were present. As expected, E2C formed a much faster migrating complex relative to the complex formed with the full-length E2 protein. The specificity of these protein-DNA complexes was demonstrated by the inability of E2 C298S, which is mutated in the DNA binding motif, to retard the probe. This mutant form of E2 did not support transient or cell-free replication, whereas the purified wild type full-length E2 protein did (data not shown). The native E2 and epitope tagged E2 functioned similarly in cell free replication assays when the same levels of the two E2 proteins are used (data not shown).

Figure 1
Gel shift analysis of the binding of HPV E2 proteins to the HPV ori

E2 binds E2BS-2 alone as a single protein dimer

Several linear DNA templates containing portions of the HPV-11 URR were generated and used as substrates for E2 binding (Fig. 2). The short fragments each contains E2BS-2, E2BS-3, 4, or E2BS-2, 3, 4. One long fragment contains all 4 sites. The optimal amounts of DNA and protein for EM visualization were empirically determined to be 100 ng and 150 ng, respectively, and were used in all the experiments unless otherwise stated. When less E2 protein was added, fewer DNAs showed bound protein, whereas more E2 led to more complexes but also DNA-protein aggregation.

Figure 2
Map of the DNA fragments used to examine E2 binding

To visualize the binding of E2 protein to HPV-11 ori DNA when only a single site is present, a 403 bp DNA fragment containing just E2BS-2 was cleaved from a pUC19-based plasmid, p7874-20 (Fig. 2B). E2BS-2 is located 229 bp from one end and 162 bp from the other end. Examination of fields of DNA revealed several structural forms. Scoring 962 molecules, 26 % of the DNA fragments were bound by protein, with the remainder appearing protein-free. Further analysis of the E2 bound DNA molecules demonstrated that 210 of the complexes consisted of a DNA molecule bound by a single protein particle located slightly off center, roughly corresponding to the location of E2BS-2 (Fig. 3A, B). The remainder of the complexes (40) consisted of DNAs with a large protein particle of variable size (presumably an E2 multimer) bound at the center or near one end (not shown) (Table 1A).

Figure 3
E2 protein forms dimers and paired dimers on DNAs containing one and two E2 binding sites
Table 1
Analysis of the binding of E2 to DNA containing E2BS-2 alone (A), E2BS-3 and E2BS-4 (B), or E2BS-2, 3, and 4 (C–F)

The majority of the E2 protein bound at E2BS-2 had a size consistent with E2 dimers (84 kDa dimer mass) based on comparison with other proteins prepared by these EM methods in previous studies. To measure the mass of the E2 particles, E2-DNA complexes were formed, fixed with glutaraldehyde and just prior to mounting on the EM substrates, creatine phosphokinase (CPK, 81 KDa) was added and the samples prepared for EM. This protein is roughly spherical and is present in solution as a monomer making it an ideal size marker in the range of 80 KDa. An example of apoferritin used in this way is shown later in Fig. 4. In the same fields, the projected areas of several CPK particles were measured and compared to the projected area of the E2 particle bound to the DNA. From the ratio of the projected areas, an estimate of the mass of the E2 particle could be calculated (Materials and Methods). The resulting value, 95 KDa, (n=14), is in good agreement with the mass of an E2 dimer plus a short segment of DNA. In this and the subsequent experiments in which a single binding site was present, there was no noticeable shortening of the DNA following protein binding.

Figure 4
E2 protein forms a hexameric particle on DNA containing three E2 binding sites

E2 binds E2BS-3 and E2BS-4 as two side-by-side dimers

The plasmid p7902-99 containing E2BS-3 and E2BS-4 was cleaved with Pvu II to generate a 482 bp fragment with the two sites centrally located (Fig. 2C). Of 355 DNAs scored, 56% were protein-bound and were divided into three classes. In the first class (46% of the E2-DNA complexes scored) the DNAs showed a single E2 particle bound near the center (not shown). In the second class (42% of the E2-DNA complexes scored) there were two side-by-side particles located near the center of the DNA (Fig. 3C, D) with no indication of any organization of the two dimers into a compact E2 tetramer following DNA binding. The size of the individual particles was indistinguishable from the dimeric particles present when the DNA contained only E2BS-2. In the third class (12% of the E2-DNA complexes scored), a large E2 multimer of variable size was centrally bound along the DNA (not shown) (Table 1B).

A disk-shaped complex of E2 forms on DNA containing E2BS-2, 3, and 4

The plasmid p7730-99 was cleaved with Pvu II to generate a 623 bp DNA fragment containing E2BS-2, E2BS-3 and E2BS-4 (Fig. 2D). E2BS-2 and E2BS-3 are separated by 64 bp while E2BS-3 and E2BS-4 are separated by 3 bp (see above). The three sites span the central 103 bp in this fragment, with 266 bp between E2BS-2 and the nearest DNA end and 254 bp from E2BS-4 to the nearest DNA end. Incubation of this DNA fragment with E2 generated a series of DNA-protein complexes. Two complexes recapitulated what was observed with DNA containing only E2BS-3 and E2BS-4: a single centrally bound E2 dimer particle, or two side-by-side E2 dimers near the center of the DNA. In addition, a new DNA-protein structure was observed which was the most abundant of the E2-DNA complexes. These molecules consisted of a DNA fragment containing a large round particle close to the center of the DNA (Fig. 4A, D–I) flanked by DNA arms, each of roughly 250 bp in length. This large complex frequently showed a hole or depression in the center, giving it a disk or ring shape. The remaining (minor) E2-DNA species consisted of larger multimers of E2 on the DNA or aggregates of the E2-DNA complexes (not shown). Titration of E2 from 40 to 320 ng relative to a constant amount of DNA (100 ng) revealed a relatively constant number of single E2 dimers on the DNA (17–24%), a low amount of side-by-side dimers (3.7 to 8%), and a frequency of disk-shaped particles that increased from 11% to 25 % (Table 1C–F). It was of interest to examine the binding of E2 to a circular DNA containing the HPV-11 ori to determine the specificity on a much larger DNA. Incubation of the 3.3 kb circular p7874-99 plasmid DNA (100 ng), which also contains E2BS-2, -3, and 4, with E2 (150 ng) led to primarily a single disk-shaped particle bound to the DNA (Fig. 4B,C). This illustrated the high specificity of E2 binding. The majority of the E2-bound DNA was in the supercoiled form. But for clear visualization of the E2 disk, complexes formed on open circular DNA are shown. This latter observation suggests that this structural remodeling of the three binding sites by E2 may be facilitated by negative supercoiling.

The disk-shaped particles contacted the DNA along one side (Fig. 4A, D–I), in contrast to E2 binding at E2BS-3 and E2BS-4 where the DNA took a straight path through the center of the side-by-side dimers (Fig. 3C,D). These results suggest that the addition of the third E2 dimer induced a conformational transition from a linear chain of 3 dimers to a ring or disk-shaped particle. The 64 bp segment between E2BS-2 and E2BS-3 would be looped out of the E2 complex but it was too small to be easily observed by these EM methods.

Comparison with the size of apoferritin (443 KDa) provided an estimate of the mass of the E2 disk on DNA. A single disk-shaped E2 particle on DNA with four apoferritin molecules in close proximity is shown in Fig. 4I. The free apoferritin particles in the background cannot be free E2 disks since they only form on long DNA, and further the apoferritin particles appear identical to apoferritin mounted on parallel grids alone. Finally the size of the apoferritin particles is in good agreement with the known diameter of 184A. The projected area of each E2 particle was measured and divided by the average projected area of the several apoferritin molecules lying nearby. From 50 such measurements, the mean projected area of the E2 complexes was 1.16 times that of apoferritin. Calculation of the masses (Materials and Methods) revealed that a spherical protein particle with this projected area would have a mass of 560 KDa. However the E2-DNA particles contain 103 bp of DNA equivalent to 70 KDa which must be subtracted. Thus, if the E2-DNA particles are spherical they would contain 5 E2 dimers; but if they are disk shaped as suggested by the images, they would have a substantially lower mass. We propose that this complex is most likely a particle containing three E2 dimers which has rearranged into a disk shaped particle with a central hole. An illustration of the proposed structure is shown in Figure 8. Interestingly, the BPV-1 E2 has also been thought to form a hexameric complex.20 In that study, a DNA bow formed by E2 proteins bound to three E2 BSs that were artificially separated from one another by 500 bp each.

Figure 8
Model of the binding of E2 and E1 to the HPV ori

DNA forms a loop when E2 and all 4 binding sites are present

The full HPV upstream regulatory region (long control region) contains 4 E2 binding sites, with E2BS-1 located 288 bp upstream from E2BS-2 (Fig. 2). The role of this E2 BS in viral DNA replication has not been examined. We asked whether E2 binding at E2BS-1 would form a complex with E2 bound at the other 3 sites to generate a new higher order complex. A1244 bp fragment was generated which contains all 4 sites (Fig. 2A) (Materials and Methods). The distances to the nearest ends are 162 bp and 640 bp. The DNA fragment was incubated with E2 protein (150 ng of E2 and 100 ng of DNA) under the same buffer conditions used above and prepared for EM. Inspection of fields of molecules revealed a variety of E2-DNA species. Several recapitulated the forms observed for DNAs containing 1 to 3 E2 binding sites, including 1 or 2 E2 dimers bound at positions consistent with the known locations of the E2 binding sites and the disk-shaped particles bound along the DNA. In addition, a new E2-dependent species was commonly observed in which the DNA was arranged into a loop with E2 protein at the node of the loop (Fig. 5A–F). Scoring 301 DNA molecules, 84% of the DNA was bound by E2 protein. Furthermore, 27% of theDNA molecules scored were arranged into a loop by the protein. The rest of the complexes were in one of the forms described in previous sections.

Figure 5
E2 protein forms loops on DNA containing all four natural E2 binding sites

Using the 1244 bp DNA (Fig. 2A) with 162 and 640 bp arms, the fraction of the total DNA length comprising the loop and the fractional length of each arm were measured in 89 looped molecules. This analysis revealed that, in 65% of the molecules, the size of the loop was consistent with looping between E2BS-1 and the cluster of E2BS-2, E2BS-3 and E2BS-4 sites, constraining the intervening DNA into a loop of 250 to 400 bp in length as shown in a histogram (Fig. 6). However, in ~1/3rd of the molecules the loop length was greater than 400 bp (Fig. 5F), with some as long as 800 bp. One possible explanation is described in the Discussion.

Figure 6
Statistical analysis of loop formation by E2 on 4BS

Inspection of the E2 particles in the looped species revealed a range of sizes and structures. Frequently there were 2 distinct equal-sized lobes (Fig. 5A, B, F, H, I) suggestive of 2 E2 dimers. In some cases (Fig. 5G) it appeared that 3 E2 dimers were arranged side-by-side in a linear manner with the DNA folding back to bind along the outside of the chain of dimers. In rare cases (Fig. 5J) there were 2 domains with one significantly larger, possibly a hexameric disk while the other was smaller likely a single E2 dimer. Overall this suggested that loop formation most commonly occurred by the binding of E2 dimers at E2BS-1 and one of the 3 downstream binding sites followed by interaction between the 2 dimers to generate a loop.

Salt, DTT, and ATP regulate DNA loop formation

To determine whether DNA looping is regulated by co-factors, E2 was incubated with the 1244 bp DNA template containing all 4 E2 binding sites (Fig. 2A). In the earlier studies using DNAs with 1 to 3 E2 binding sites, we noted that inclusion of 2 mM ATP in the absence of Mg++ ions provided more consistent results and somewhat higher levels of binding than when it was left out and thus ATP was included in the work above. ATP is essential for the loading of E1 in an E2-dependent fashion, although any direct effect of ATP on E2 is not known.23 Incubation of the full-length E2 with the DNA containing 4 E2 sites as described above (Fig. 5) in the presence of 2 mM ATP resulted in 27% of the total molecules scored being arranged into E2-mediated loops (Table 2). When ATP was omitted from the incubation, the level of looping dropped to 11%. If however 1 mM DTT was included (in the absence of ATP), then the level of looping was restored (Table 2). Looping was almost completely abolished (3% loops) by raising the salt in the incubation to 50 mM KCl (Table 2). In the latter case, while looping was abolished, 60% of the DNA showed E2 particles bound along the linear DNA. Further inspection of these results (Table 2) revealed that, in the presence of ATP or DTT, there were fewer DNA molecules with no protein bound (16% and 7% respectively) with a corresponding higher level of looping. In contrast, in the absence of ATP or in the presence of salt, there were more protein-free DNAs (48% and 38% respectively) and fewer loops. These observations point to complex ionic and co-factor requirements for loop formation. A model, which may help explain these observations, is described in the Discussion.

Table 2
Analysis of the binding of E2 on DNA containing all 4 four BS sites

The E2 amino terminal trans-acting domain is required for DNA looping

The 83 a.a. E2 carboxyl-terminal fragment is ~9 KDa which is too small to be detected by the EM methods used here bound to DNA. Based on previous work in this laboratory with DNA binding proteins of increasing size, a tetramer of the E2C fragment would be the smallest oligomeric form that we would expect to be visualizable bound to DNA by these shadow-casting methods. When E2C was incubated with the 1244 bp DNA containing all 4 E2 binding sites in a buffer containing 50 mM KCl and no ATP, tiny protein particles were observed bound to 81% of the DNA molecules (arrows Fig. 7A–C) and their positions corresponded roughly to the E2 BSs. When the incubations contained 2 mM ATP and no salt, these tiny particles were also present on the DNA (84%) (Fig. 7E) but larger particles were also observed (Fig. 7D,F). These large particles must represent some higher multimers of the E2C fragment. Under either condition, only a few DNAs were arranged into loops, ranging from 1 to 6% of the total DNA. In the cases of looping the protein complex at the node of the loop was large, such as the particles in Fig. 7D,F. These observations suggest that DNA looping could be attributable to an interaction between the amino terminal trans-acting domain of pairs of E2 dimers bound to E2BS-1 and one of the downstream sites, in agreement with the report for BPV-1 E2.20 This interpretation also agrees with the amino-terminal interaction model based on the X ray crystal structures of HPV-16 E2.24

Figure 7
Visualization of E2C bound to DNA containing all binding sites


In this study we employed transmission electron microscopy to examine the remodeling of the HPV-11 replication origin by the E2 protein. This involves local and long range interactions between E2 and DNA over a distance of ~500 bp, and we observed the generation of both small and large DNA-protein particles and looped species. While the HPV ori has been known to bind both E2 and the replicative helicase E1, no previous studies have been carried out to determine how E2 interacts globally with the URR segment containing all 4 binding sites. We can summarize observations of several thousand individual molecules as follows: E2 binds to each of the 4 sites as single protein dimers at low protein concentration. When just the 2 closely spaced sites are present, a pair of E2 dimers is arranged side-by-side on the DNA. With the addition of the third member of the cluster of three sites, a rapid conversion of the E2-DNA complex into a disk-shaped particle occurs, suggestive of a ring-like trimer of E2 dimers (or hexamer) with DNA wound around the ring. Finally upon inclusion of the distal E2BS-1, DNA loops 250 to 400 bp in size formed between E2BS-1 and one of 3 closely spaced sites. Looping frequently involves 2 dimers, one at E2BS-1 and another at one of the downstream sites. These studies revealed two DNA remodeling events upon binding by E2: the generation of a ring trimer of E2 dimers and the formation of long range DNA loops. These structures likely function to control the binding of the E1 helicase and access of other proteins to the ori.

Hexameric E2 ring formation on the origin

The formation of the disk-shaped E2 oligomers must be rapid, as we never observed 3 side-by-side dimers under the standard binding conditions. Mass analysis and modeling suggested that the disk or ring is composed of 3 E2 dimers and ~100 bp of DNA. The observation that the DNA enters and exits from a single point on the side of the particle suggests that the linear chain of 3 E2 dimers coils into a ring with the DNA along the outside of the ring. A trimer of dimers has been observed in the crystal structure of the carboxyl terminal DNA binding domain of the BPV-1 E2 protein.19,25 The 3 E2C dimers are cross-linked by 3 disulfide bonds between neighboring E2 dimers. We have no information as to whether the HPV-11 E2 ring complex described in this study also involves disulfide bonds between neighboring dimers. However we note that addition of DTT to the binding buffer did not abolish E2 disk formation, suggesting disulfide bond formation is not essential. The validity of this argument was further confirmed by the fact that the DNA binding activity of E2 increased in the presence of DTT. The crystal structure of BPV-1 E2 revealed that one of the cysteine residues involved in trimer formation of E2C is also essential for the DNA binding activity. Thus, disulfide formation could inhibit the DNA binding of the enzyme as well. Within this hexameric ring is a 64 bp DNA segment containing the binding site for the E1 replicative helicase, and this segment would not be expected to be bound by E2. Rather it may either be looped into the center of the E2 ring or perhaps looped out on the surface presenting the 64 bp segment to E1 (Fig. 8). The strain of bending the DNA helix into a tight 64 bp loop would be expected to facilitate E1 loading and denaturing the ori sequence. Negative supercoiling of the DNA template should and did promote the wrapping of the 3 E2 dimers into the compact hexameric particle, as a toroidal loop is topologically equivalent to a supercoil in covalently closed DNA molecules. This compact E2-ori particle may have similarities in the DnaA complex that forms at the E. coli ori26 or the lambda O-some formed by the binding of the O protein at the lambda replication ori.27

Examination of the binding of E2 when there were two adjacent sites (Table 1) revealed an apparent lack of cooperativity in binding. DNAs were seen with one or two dimers bound rather than only two dimers or none (cooperative binding). This is in agreement with the work of Tan and colleagues28 who noted a lack of cooperativity for HPV16 E2 and in contrast to the work of Monini and colleagues29 who observed cooperative binding for BPV1 E2. The observation that inclusion of 2 mM ATP led to a 2-fold enhancement in E2 binding seemed curious as magnesium was not added and no ATPase activity has been detected or reported for E2 protein. It remains possible however that E2 weakly binds ATP and that upon entry of E1 (Fig. 8) E2 acts as an ATP donor presenting a required substrate to E1 for its ensuing unwinding of the DNA.

DNA looping by E2

Protein-mediated DNA looping has been visualized previously, beginning with the demonstration of loops generated by lambda repressor.30 The activation of the ori of the E. coli R6K plasmid has been shown to involve long range DNA looping mediated by the ori binding protein RepA.31,32, DNA looping also occurs within the latency replication ori (oriP) of the human Epstein-Barr virus (EBV).33,34 OriP contains two clusters of binding sites for the ori binding protein EBNA-1 separated by ~1 kb, and looping between the two clusters mediated by EBNA-1 may serve to generate a large DNA-protein complex that controls switching the virus from latent to lytic states. DNA looping has been observed with BPV-1 E2 protein bound to BPV-1 ori fragments.20 In that study, a tiny loop was occasionally observed between two sites spaced very close together, and larger loops generated by BPV-1 E2 were also noted but these involved an artificial template that did not reflect the spacing of sites in the BPV ori.

DNAs containing E2-mediated loops were the most frequently observed species when the ori DNA contains all 4 E2BS’s. Because looping was reduced 5–7 fold with E2C which lacks the amino-terminal trans-acting domain, looping may be attributed to interactions between the amino termini between pairs of dimers, one bound at site 1, the other at one of the three downstream sites. Loop formation in which a ring/disk shaped hexamer of E2 encompassing E2BS-2,-3, and 4 interacted with an E2 dimer bound at E2BS-1 was very uncommon, suggesting the long distance interaction involving one of E2 dimers at the downstream site may have prevented its incorporation into a hexameric ring with two E2 dimers bound at neighboring sites.

The HPV-11 protein E1 binds to the ori as a hexameric or dihexameric ring.14,15 It is tempting to speculate that the hexameric E2 protein bound on HPV-11 ori at E2BS-2, -3 and -4 plays an important role in recruitment of the HPV-11 E1 protein. The 64 bp segment between E2BS-2 and E2BS-3 may undergo a great change in torsion when the linear chain of E2 dimers bound at E2BS-2,-3-, and -4 coils around to form the disk-shaped particle with the E1 binding site pinched out, as we illustrate in Fig. 8. This would facilitate the assembly of the E1 dihexamer, whereupon E2 would be released from the ori.17 Further high resolution EM studies will be focused on examination of the recruitment of E1 to the ori in the presence of E2. In this model, the DNA looping observed when E2 was also bound to E2BS-1 DNA might provide a mechanism to regulate DNA replication by competing for the E2 hexamer disk formation on the ori, thereby inhibiting the recruitment of E1 to the ori and initiation of replication. The large DNA loop does not induce steric strain as the small DNA loop does and hence may not be able to facilitate E1 binding and DNA melting. It is conceivable that E2 protein may increase along with viral DNA copy number in the differentiated keratinocytes. Upon achieving certain high protein level, E2 would cause viral DNA looping, thereby inhibiting further viral DNA amplification. This would then permit the expression of late gene encoding capsid proteins for virion assembly.

A model for E2 binding to DNA

The observation of a minor but significant fraction (1/3rd) of the looped molecules in which the size of the loop was greater than the distance between E2BS-1 and the cluster of 3 adjacent binding sites may reflect the mode by which E2 scans DNA for its binding sites. E2 dimers may bind loosely to DNA and then scan along the DNA until a high affinity binding site is located. In this model, a transitory loop might form between an E2 dimer bound at, for example E2BS-4 and another E2 dimer bound in a scanning mode beyond E2BS-1 near the far terminus of the DNA fragment generating a very large loop. In this model, loop formation serves to stabilize the E2 dimer bound in the scanning mode at a non-consensus binding site until it is finally captured by the specific binding sites in a diffusion driven reaction. This model might also explain the larger protein complexes observed on some looped molecules if loading of additional E2 within the loop followed by scanning resulted in the trapping of more E2 dimers at the node of the loop.

Materials and Methods

Plasmids and protein

HPV-11 E2 expression construct and purification has been described previously.35 The E2 protein from pRSET-11E2 was tagged at the amino terminus with an 8-amino acid epitope derived from a cytomegalovirus-encoded protein, pp65 (a phosphor-protein encoded by the UL83 gene of cytomegalovirus). The replication activities by the epitope tagged E2 and the native E2 proteins were similar (Data not shown). Construction of the HPV-11 E2 C298S mutant and the E2C protein used the same vector. Both clones were constructed by PCR mutagenesis and cloned into the pRSET expression vector. E2C spans the carboxyl terminus of E2 (residues 285–367). Purified proteins were tested in electrophoretic mobility shift assays (EMSA) as described.36 The probes were end-labeled double-stranded synthetic oligonucleotides spanning nucleotides 7902-7933/1-92 containing E2 BS-3 and BS-4. A DNA substrate containing all four E2 BS’s was generated by PCR using the following set of primers: a 1244 bp long substrate with the 5′-TACGCCAGCTGGCGAAAGG-3′ and 5′-GCTTTACACTTTATGCTTCCGG-3′. The primers for this substrate were designed to regions of the pUC19 plasmid flanking the HPV-11 URR fragment in the p7072-99 plasmid. Plasmid p7072-99 contains the HPV-11 URR fragment (nucleotides [nt] 7072 to 7933/1 to 99 between the Hind III and Bam HI sites of pUC 19.37 The reactions were performed with a Qiagen Thermocycler using standard Taq Polymerase reaction conditions according to the manufacturer’s protocol (Invitrogen). The PCR product was separated on 0.8 % agarose gels run in 1x Tris-Borate buffer (TBE) for 1.5 hours at 125 Volts. The correct DNA band was excised from the gel and purified using a gel purification kit (Qiagen). The final concentration of the DNA was adjusted to 50 ng/μl. Plasmidp7730-99, p7874-20, and p7902-99 contain HPV-11 nucleotides 7730 to 7933/1 to 99, 7874 to 7933/1 to 20, and 7902 to 7933/1 to 99, respectively.5 These sequences contain E2 BS-2, -3, -4; E2 BS-2; and E2 BS-3, -4 respectively and are illustrated in Fig. 2.

DNA binding reactions

Binding assays were conducted with 100 ng of linear DNA and, unless otherwise noted, 150 ng of E2 protein in 20 μl of buffer containing 20 mM HEPES, pH 7.5, 2 mM ATP. The mixture of DNA and E2 protein was incubated for 20 min at 37°C, and then treated with glutaraldehyde (0.6 % (v/v) final concentration) for 5 min at room temperature. DNA-E2 complexes were separated through 2 ml columns of 6% agarose beads (Agarose Bead Technologies Inc.) equilibrated with 10 mM Tris-HCl, pH8.0, 0.1 mM EDTA.

Electron microscopy

Thin carbon foils supported by 400 mesh copper grids were treated with a glow discharge for 45 seconds as described previously.38 Aliquots from the agarose bead column fractions were adsorbed for 3 min to the grids in a buffer containing 2 mM spermidine. The grids were washed sequentially with water and graded water/ethanol solutions to 100 % ethanol, followed by air-drying and rotary shadow-casting with tungsten at 1 × 10−6 torr. Philips CM12 and Tecnai 12 instruments were used at 40 KV, and images were recorded on film or digitally with a GATAN 794 slow scan camera (CM12) and GATAN Ultrascan 4000SP (Tecnai 12). The images were analyzed with Gatan software, and contrast was adjusted with Adobe Photoshop software.

The masses of E2-DNA particles were determined as described.39 Briefly, internal size markers consisting of proteins of known molecular weight were added to the DNA-E2 complexes prior to preparation for EM. Fields containing the E2-DNA complexes with internal size marker proteins lying nearby were photographed using the GATAN 794 camera. Using the NIH Image software, the relative projected areas of the standard proteins and the E2 complex bound to the DNA were determined. The ratio of the projected areas was converted to relative masses using the formula [(Mass1/Mass2)=(Area1/Area2)39


This work was supported in part by USPHS grants to JDG (GM31819, ES 13773) and to LTC (CA83679).


Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.


1. Yang L, Li R, Mohr IJ, Clark R, Botchan MR. Activation of BPV-1 replication in vitro by the transcription factor E2. Nature. 1991;353:628–32. [PubMed]
2. Ustav M, Stenlund A. Transient replication of BPV-1 requires two viral polypeptides encoded by the E1 and E2 open reading frames. Embo J. 1991;10:449–57. [PubMed]
3. Chiang CM, Ustav M, Stenlund A, Ho TF, Broker TR, Chow LT. Viral E1 and E2 proteins support replication of homologous and heterologous papillomaviral origins. Proc Natl Acad Sci U S A. 1992;89:5799–803. [PubMed]
4. Kuo SR, Liu JS, Broker TR, Chow LT. Cell-free replication of the human papillomavirus DNA with homologous viral E1 and E2 proteins and human cell extracts. J Biol Chem. 1994;269:24058–65. [PubMed]
5. Liu JS, Kuo SR, Broker TR, Chow LT. The functions of human papillomavirus type 11 E1, E2, and E2C proteins in cell-free DNA replication. J Biol Chem. 1995;270:27283–91. [PubMed]
6. Van Tine BA, Dao LD, Wu SY, Sonbuchner TM, Lin BY, Zou N, Chiang CM, Broker TR, Chow LT. Human papillomavirus (HPV) origin-binding protein associates with mitotic spindles to enable viral DNA partitioning. Proc Natl Acad Sci U S A. 2004;101:4030–5. [PubMed]
7. Dao LD, Duffy A, Van Tine BA, Wu SY, Chiang CM, Broker TR, Chow LT. Dynamic localization of the human papillomavirus type 11 origin binding protein E2 through mitosis while in association with the spindle apparatus. J Virol. 2006;80:4792–800. [PMC free article] [PubMed]
8. Lehman CW, Botchan MR. Segregation of viral plasmids depends on tethering to chromosomes and is regulated by phosphorylation. Proc Natl Acad Sci U S A. 1998;95:4338–43. [PubMed]
9. Skiadopoulos MH, McBride AA. Bovine papillomavirus type 1 genomes and the E2 transactivator protein are closely associated with mitotic chromatin. J Virol. 1998;72:2079–88. [PMC free article] [PubMed]
10. Ilves I, Kivi S, Ustav M. Long-term episomal maintenance of bovine papillomavirus type 1 plasmids is determined by attachment to host chromosomes, which Is mediated by the viral E2 protein and its binding sites. J Virol. 1999;73:4404–12. [PMC free article] [PubMed]
11. You J, Croyle JL, Nishimura A, Ozato K, Howley PM. Interaction of the bovine papillomavirus E2 protein with Brd4 tethers the viral DNA to host mitotic chromosomes. Cell. 2004;117:349–60. [PubMed]
12. Stenlund A. Initiation of DNA replication: lessons from viral initiator proteins. Nat Rev Mol Cell Biol. 2003;4:777–85. [PubMed]
13. Chow LT, Broker TR. Mechanism and regulation of papillomavirus DNA replication. In: Campo MS, editor. Papilloma Research: From Natural History to Vaccines and Beyond. Caister Academic Press; 2006. p. 53.
14. Liu JS, Kuo SR, Makhov AM, Cyr DM, Griffith JD, Broker TR, Chow LT. Human Hsp70 and Hsp40 chaperone proteins facilitate human papillomavirus-11 E1 protein binding to the origin and stimulate cell-free DNA replication. J Biol Chem. 1998;273:30704–12. [PubMed]
15. Lin BY, Makhov AM, Griffith JD, Broker TR, Chow LT. Chaperone proteins abrogate inhibition of the human papillomavirus (HPV) E1 replicative helicase by the HPV E2 protein. Mol Cell Biol. 2002;22:6592–604. [PMC free article] [PubMed]
16. Sverdrup F, Khan SA. Replication of human papillomavirus (HPV) DNAs supported by the HPV type 18 E1 and E2 proteins. J Virol. 1994;68:505–9. [PMC free article] [PubMed]
17. Abbate EA, Berger JM, Botchan MR. The X-ray structure of the papillomavirus helicase in complex with its molecular matchmaker E2. Genes Dev. 2004;18:1981–96. [PubMed]
18. Zou N, Lin BY, Duan F, Lee KY, Jin G, Guan R, Yao G, Lefkowitz EJ, Broker TR, Chow LT. The hinge of the human papillomavirus type 11 E2 protein contains major determinants for nuclear localization and nuclear matrix association. J Virol. 2000;74:3761–70. [PMC free article] [PubMed]
19. Hegde RS. The papillomavirus E2 proteins: structure, function, and biology. Annu Rev Biophys Biomol Struct. 2002;31:343–60. [PubMed]
20. Knight JD, Li R, Botchan M. The activation domain of the bovine papillomavirus E2 protein mediates association of DNA-bound dimers to form DNA loops. Proc Natl Acad Sci U S A. 1991;88:3204–8. [PubMed]
21. Harris SF, Botchan MR. Crystal structure of the human papillomavirus type 18 E2 activation domain. Science. 1999;284:1673–7. [PubMed]
22. Wang Y, Coulombe R, Cameron DR, Thauvette L, Massariol MJ, Amon LM, Fink D, Titolo S, Welchner E, Yoakim C, Archambault J, White PW. Crystal structure of the E2 transactivation domain of human papillomavirus type 11 bound to a protein interaction inhibitor. J Biol Chem. 2004;279:6976–85. [PubMed]
23. Gillette TG, Lusky M, Borowiec JA. Induction of structural changes in the bovine papillomavirus type 1 origin of replication by the viral E1 and E2 proteins. Proc Natl Acad Sci U S A. 1994;91:8846–50. [PubMed]
24. Antson AA, Burns JE, Moroz OV, Scott DJ, Sanders CM, Bronstein IB, Dodson GG, Wilson KS, Maitland NJ. Structure of the intact transactivation domain of the human papillomavirus E2 protein. Nature. 2000;403:805–9. [PubMed]
25. Hegde RS, Wang AF, Kim SS, Schapira M. Subunit rearrangement accompanies sequence-specific DNA binding by the bovine papillomavirus-1 E2 protein. J Mol Biol. 1998;276:797–808. [PubMed]
26. Crooke E, Thresher R, Hwang DS, Griffith J, Kornberg A. Replicatively active complexes of DnaA protein and the Escherichia coli chromosomal origin observed in the electron microscope. J Mol Biol. 1993;233:16–24. [PubMed]
27. Dodson M, Echols H, Wickner S, Alfano C, Mensa-Wilmot K, Gomes B, LeBowitz J, Roberts JD, McMacken R. Specialized nucleoprotein structures at the origin of replication of bacteriophage lambda: localized unwinding of duplex DNA by a six-protein reaction. Proc Natl Acad Sci U S A. 1986;83:7638–42. [PubMed]
28. Tan SH, Leong LE, Walker PA, Bernard HU. The human papillomavirus type 16 E2 transcription factor binds with low cooperativity to two flanking sites and represses the E6 promoter through displacement of Sp1 and TFIID. J Virol. 1994;68:6411–20. [PMC free article] [PubMed]
29. Monini P, Grossman SR, Pepinsky B, Androphy EJ, Laimins LA. Cooperative binding of the E2 protein of bovine papillomavirus to adjacent E2-responsive sequences. J Virol. 1991;65:2124–30. [PMC free article] [PubMed]
30. Griffith J, Hochschild A, Ptashne M. DNA loops induced by cooperative binding of lambda repressor. Nature. 1986;322:750–2. [PubMed]
31. Mukherjee S, Erickson H, Bastia D. Detection of DNA looping due to simultaneous interaction of a DNA-binding protein with two spatially separated binding sites on DNA. Proc Natl Acad Sci U S A. 1988;85:6287–91. [PubMed]
32. Chattoraj DK, Mason RJ, Wickner SH. Mini-P1 plasmid replication: the autoregulation-sequestration paradox. Cell. 1988;52:551–7. [PubMed]
33. Frappier L, O’Donnell M. Epstein-Barr nuclear antigen 1 mediates a DNA loop within the latent replication origin of Epstein-Barr virus. Proc Natl Acad Sci U S A. 1991;88:10875–9. [PubMed]
34. Deng Z, Lezina L, Chen CJ, Shtivelband S, So W, Lieberman PM. Telomeric proteins regulate episomal maintenance of Epstein-Barr virus origin of plasmid replication. Mol Cell. 2002;9:493–503. [PubMed]
35. Lin BY, Ma T, Liu JS, Kuo SR, Jin G, Broker TR, Harper JW, Chow LT. HeLa cells are phenotypically limiting in cyclin E/CDK2 for efficient human papillomavirus DNA replication. J Biol Chem. 2000;275:6167–74. [PubMed]
36. Hou SY, Wu SY, Chiang CM. Transcriptional activity among high and low risk human papillomavirus E2 proteins correlates with E2 DNA binding. J Biol Chem. 2002;277(47):45619–29. [PubMed]
37. Chin MT, Hirochika R, Hirochika H, Broker TR, Chow LT. Regulation of human papillomavirus type 11 enhancer and E6 promoter by activating and repressing proteins from the E2 open reading frame: functional and biochemical studies. J Virol. 1988;62:2994–3002. [PMC free article] [PubMed]
38. Griffith JD, Christiansen G. Electron microscope visualization of chromatin and other DNA-protein complexes. Annu Rev Biophys Bioeng. 1978;7:19–35. [PubMed]
39. Griffith JD, Makhov A, Zawel L, Reinberg D. Visualization of TBP oligomers binding and bending the HIV-1 and adeno promoters. J Mol Biol. 1995;246:576–84. [PubMed]