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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
J Mol Biol. Author manuscript; available in PMC 2010 September 11.
Published in final edited form as:
PMCID: PMC2756645
NIHMSID: NIHMS131151

Ezrin induces long-range interdomain allostery in the scaffolding protein NHERF1

Abstract

Scaffolding proteins are molecular switches that control diverse signaling events. The scaffolding protein NHERF1 assembles macromolecular signaling complexes and regulates the macromolecular assembly, localization and intracellular trafficking of a number of membrane ion transport proteins, receptors, and adhesion/antiadhesion proteins. NHERF1 begins with two modular protein-protein interaction domains—PDZ1 and PDZ2—and ends with a C-terminal domain. This C-terminal domain binds to ezrin, which in turn interacts with cytosekeletal actin. Remarkably, ezrin binding to NHERF1 increases the binding capabilities of both PDZ domains. Here we use deuterium labeling and contrast variation neutron scattering experiments to determine the conformational changes in NHERF1 when it forms a complex with ezrin. Upon binding to ezrin, NHERF1 undergoes significant conformational changes in the region linking PDZ2 and its C-terminal ezrin-binding domain, as well as in the region linking PDZ1 and PDZ2, involving very long-range interactions over 120 Å. The results provide a structural explanation, at mesoscopic scales, of the allosteric control of NHERF1 by ezrin as it assembles protein complexes. Because of the essential roles of NHERF1 and ezrin in intracellular trafficking in epithelial cells, we hypothesize that this long-range allosteric regulation of NHERF1 by ezrin enables the membrane-cytoskeleton to assemble protein complexes that control cross-talk and regulate the strength and duration of signaling.

Introduction

The transduction of biological signals is tightly controlled by the dynamic assembly and disassembly of protein complexes. Scaffolding proteins play critical roles in regulating of diverse signaling events 1; 2. The scaffolding protein Na+/H+ exchanger regulatory factor 1 (NHERF1), also known as ezrin binding protein 50 (EBP50), recruits signaling protein partners and directs proteins to specific cellular locations in mammalian epithelial cells. NHERF1 belongs to the Na+/H+ exchanger regulatory factor family of proteins 3; 4. Members of this protein family contain two or more copies of modular PDZ (PSD-95/Discs-large/ZO-1) domains that are capable of binding to specific amino acid motifs residing in the cytoplasmic portion of a number of transmembrane proteins 5; 6. NHERF1 has two PDZ domains, PDZ1 and PDZ2, and a C-terminal domain, see Figure 1. The NHERF1 PDZ domains bind to a growing list of membrane proteins including ion transport proteins, tyrosine kinase receptors, and the G-protein coupled receptors, for review see 7; 8.

Figure 1
Thermodynamic cycle analysis reveals FERM induced long-range inter-domain allostery in the scaffolding protein NHERF1. FERM binding to NHERF1 increases the binding affinities of both PDZ1 and PDZ2 for C-CFTR. (A) The “coupling” energy ...

NHERF1 regulates the intracellular trafficking and signaling of membrane proteins to which it binds. For example, NHERF1 and ezrin anchor the cystic fibrosis transmembrane conductance regulator (CFTR) to the actin cytoskeleton, and promote the retention of CFTR in the apical membrane of epithelial cells and the interaction of CFTR with other apical membrane proteins 9; 10. Notably, recent studies show that NHERF1 increases the cell surface expression of a disease-causing mutant of CFTR with a deletion at amino acid Phe508 (ΔF508) 11; 12; 13. The ΔF508 mutant, responsible for 80% of the cases of the genetic disease cystic fibrosis, is trapped in the endoplasmic reticulum after biosynthesis and fails to reach the cell membrane to perform normal functions as a chloride ion channel. NHERF1 also assembles a signaling macromolecular complex comprised of CFTR and the G-protein coupled beta 2 adrenergic receptor. This complex is believed to stimulate the CFTR ion channel by the β2AR receptor 14; 15. Further, NHERF proteins can also function as molecular switches that not only regulate the intracellular trafficking but also alter that the specific pathways the parathyroid hormone receptor 1 (PTH1R) signals through 16; 17.

An important function of NHERF1 is that its carboxyl-terminal domain binds to ezrin 18. Ezrin is a member of the ezrin/radxin/moesin (ERM) family membrane-cytoskeleton linker proteins that plays vital roles in cytoskeletal-related events such as cell polarization, intracellular trafficking, cell adhesion, cell survival, cell motility, as well as tumor metastasis 19; 20; 21. Ezrin and other ERM proteins are regulated by head-to-tail like intramolecular interactions between the N-terminal 4.1-ezrin/radixin/moesin (FERM) domain and the C-terminal actin binding domains 22; 23. In the inactive state, the FERM domain is masked by the actin binding domain. Ezrin becomes activated when these intramolecular interactions are disrupted upon activation by phospholipid phosphatidylinositol-4,5-bisphosphate (PIP2) binding and phosphorylation at T567 by PKC, rho kinase, AKT2, and/or at Tyr353 in response to epidermal growth factor treatment 24; 25; 26. The activated ezrin binds to target membrane proteins either directly or indirectly through NHERF1 or 2 by the FERM domain while the ezrin C-terminus interacts with the actin filament, forming a communication linkage between the cell membrane and the cytoskeletal network.

It is becoming increasingly clear that NHERF1 and ezrin operate in a cooperative fashion to assemble protein complexes at the cell membrane. A study by Weinman et al. shows that expressing NHERF1 without the carboxy-terminal ERM-binding (EB) domain leads to the internalization of Na+/H+ exchanger 3 and abolishes ion transport activities of NHE3 27. Truncation of the EB domain results in loss of functional expression of the sodium-potassium-ATPase transporter at the cell membrane 28. Similarly, expressing NHERF1 in cells impedes antagonist-induced endocytosis of PTH1R, but deleting the EB domain of NHERF1 results in otherwise inactive ligands to internalize PTH1R 29. A recent study by Mahon finds that ezrin binding to NHERF1 is necessary to promote the co-localization and functional expression of a heterogeneous complex of the parathyroid hormone receptor and the sodium-phosphate cotransporter 2a at the apical membrane 30. These studies show that ezrin and the ezrin-binding domain in NHERF1 are essential to regulate the intracellular trafficking and function of ion transport proteins and receptors at the cell membrane.

We have previously shown that ezrin positively regulates NHERF1, enabling it to assemble protein complexes cooperatively 31. In particular, when the FERM domain of ezrin binds to the carboxy-terminal EB domain of NHERF1 with high affinity (with Kd=19nM), the binding affinity of PDZ2 for the 70 amino acid residue C-terminal domain of CFTR (C-CFTR), which contains a type I carboxy-terminal PDZ binding motif –DTRL, increases by 26 fold 31, see Figure 1. As a result of ezrin binding, the stoichiometry of the full-length NHERF1 binding to C-CFTR is increased from 1:1 to 1:2. Although the binding affinity of PDZ1 for C-CFTR is already high with Kd=298 nM, this interaction is enhanced still further when ezrin is bound to NHERF1. A thermodynamic cycle analysis indicates that ezrin positively modulates the long-range intramolecular domain-domain interactions in NHERF1, and controls NHERF1 to assemble membrane signaling complexes allosterically, see Figure 1 and legend.

The structures of the isolated PDZ1 and PDZ2 domains of NHERF1, and the interactions of PDZ1 with the carboxy-terminal peptides of membrane receptors and channels have been determined by X-ray crystallography 32; 33; 34. X-ray crystallography studies have determined the binding sites in NHERF1 and FERM 18; 35. The crystal structure of radixin FERM domain in complex with the EB peptide of NHERF1 reveals that the extreme C-terminal 11 residues (a.a. 348-358) of NHERF1 adopt an amphipathic α-helix with a MDWxxxxx (L/I)Fxx(L/F) motif when bound to the pocket in the FERM subdomain C through hydrophobic interactions. However, the mechanism of long-range allosteric regulation of NHERF1 by ezrin to assemble protein complexes is not known.

To determine the mechanisms of ezrin regulating NHERF1 to assemble protein complexes cooperatively, we have used small angle neutron scattering (SANS) to determine domain-domain conformational changes in NHERF1 upon binding to the FERM domain of ezrin that mimics the activated ezrin. Using deuterium labeling and contrast variation small angle neutron scattering, one can determine the conformational changes of a protein component in a protein complex, as well as the composite structure of the complex 36; 37; 38; 39; 40. The results show that ezrin binding triggers long-range inter-domain conformational changes in NHERF1, which provide a structural explanation for increased binding affinities of both PDZ domains for their respective target proteins.

Results

NHERF1 adopts an elongated conformation, with three distinct domains having close contacts between the PDZ2 and the C-terminal domains

Previously, we have performed biochemical, light scattering, and solution small angel X-ray scattering (SAXS) experiments to determine domain-domain interactions in NHERF1 41. These results are summarized here for the purpose of comparing with the present SANS studies that determine the conformational changes in NHERF1 upon binding to the FERM domain of ezrin. Static light scattering (SLS) experiments, which determine the absolute molecular mass, show that the full-length NHERF1 is a monomer in solution (Table I and Figure S1). The monomeric full-length NHERF1 is elongated, as shown by the length distribution function P(r) obtained from SAXS experiments (Figure 2B). The radius of gyration of NHERF1 is Rg=40.9±0.6 Å and the maximum dimension is Dmax=140 Å. The hydrodynamic radius of the full-length NHERF1 is Rh=41.0±0.4 Å, which is slightly larger than Rg. The 3-D shape of NHERF1 reconstructed ab initio from SAXS has three distinct lobes, with two lobes in close contact with each other but the third lobe is well separated from these two lobes (Figure 5A).

Figure 2
Comparing the conformation of NHERF1 with dNHERF1, PDZ2CT with dPD2CT, NHERF1·FERM with dNHERF·FERM, and PDZ2CT with dPDZ2CT by SAXS. (A) I(Q) of NHERF1 (open square) and dNHERF1 (filled square). I(Q) of PDZ2CT (open circle) and dPDZ2CT ...
Figure 5
Comparing the 3-D shape of NHERF1 in solution with that of dNHERF1 in complex with FERM (upper panel); comparing the 3-D shape of PDZ2CT in solution with that of dPDZ2CT in complex with FERM (lower panel). The crystal structures of PDZ1 (PDB code: 1G9O) ...
Table I
Size and molecular mass of NHERF1, PDZ2CT, NHERF1·FERM, and PDZ2CT·FERM with their deuterium labeled counterparts

When the FERM domain of ezrin is bound to the C-terminal EB domain of NHERF1, the PDZ2 domain experiences a twenty-fold increase in binding affinity for target proteins31. To determine if there are intramolecular domain-domain interactions between PDZ2 and the C-terminal (CT) domain, we have generated a truncated construct of PDZ2 plus the intact C-terminal domain (PDZ2CT) (Figure 1B and and1C).1C). PDZ2CT is monomeric in solution, as determined by SLS (Table I). P(r) of PDZ2CT shows a shoulder at 45.8 Å, suggesting that there are two distinct lobes in PDZ2CT (Figure 2C and Figure 4D). The center-of-mass distance between the two domains can be estimated by the position of the shoulder to be 45.8 Å42. The ab initio reconstructed 3-D shape of PDZ2CT has two lobes (Figure 5C). The center-of-mass distance between the two lobes can also be measured in the 3-D map to be 45.3 Å. In the 3-D shape of PDZ2CT, PDZ2 and CT are in contact with each other. By comparing the 3-D shape of PDZ2CT and that of the full-length NHERF1, we could assign the middle lobe as PDZ2, the lobe at the right side as the CT domain, and the lobe at the left side PDZ1.

Figure 4
FERM binding induces significant conformational changes in dNHERF1 and dPDZ2CT. (A) Comparing the SAXS data of NHERF1 in solution (open square) with the SANS data of dNHERF1 in the complex in 42% D2O (filled square). The insets are the Guinier plots. ...

Our protein kinase C (PKC) phosphorylation experiments also indicate that there domain-domain interactions in PDZ2CT 41. Using site-directed mutagenesis and PKC phosphorylation experiments, we have found that PKC phosphorylates two amino acid residues Ser-339 and Ser-340 in the linker region between PDZ2 and the EB domain. Another Serine 162 in PDZ2, located above the peptide binding pocket, can be phosphorylated by PKC in the truncated PDZ2 domain, but this residue is specifically protected from being phosphorylated by the intact CT domain in PDZ2CT. These scattering and biochemical results show that the PDZ2 and CT domains are in physical contact with each other, but PDZ1 is well separated from PDZ2 and CT.

Contrast match SANS reveals long-range conformational changes in NHERF1 upon binding to the FERM domain of ezrin

The structure of a protein component in a multi-component complex can be determined using selective deuterium labeling and contrast variation SANS, because the neutron scattering lengths of hydrogen and deuterium are quite different. The neutron scattering intensity from a complex composed of the deuterium labeled and the unlabeled components is expressed as 36:

I(Q)=ΔρD2ID(Q)+ΔρH2IH(Q)+ΔρDΔρHIDH(Q)
(1)

where ΔρD = ρDρo is the neutron scattering length density contrast between the deuterated component and that of the buffer, and ΔρH = ρHρo is the neutron scdattering length density contrast between the hydrogenated component and that of the buffer. ID(Q) and IH(Q) are the scattering from the deuterium labeled and the hydrogenated component, respectively, and IDH(Q) is the term reflecting the interference scattering between the hydrogenated and the deuterated components. The neutron scattering length density of the buffer ρo changes with the volume fractions of D2O, and ΔρD or ΔρH changes accordingly. When the neutron scattering length density of the buffer matches that of the unlabeled protein component or that of the deuterium labeled component, Eq. (1) shows that structural information about the deuterium labeled or the unlabeled component can be obtained. Contrast variation neutron scattering experiments have also been applied to determine the structure of protein-nucleic acid and protein-lipid complexes 43; 44 because the neutron scattering length density of a protein is different from that of a nucleic acid or lipid membranes.

We have produced deuterium labeled NHERF1 and PDZ2CT for the contrast variation SANS experiments. The non-exchangeable deuterium contents for the deuterium labeled NHERF1 and PDZ2CT are 67% and 73%, respectively, as determined by mass spectroscopy. These partially deuterated proteins are designated as dNHERF1 and dPDZ2CT. In 42% D2O solution, the neutron scattering length density of the unlabeled FERM ρH matches that of the buffer solution, ΔρH ≈ 0. In 100% D2O solution, the neutron scattering length density of the partially labeled dNHERF1 or dPDZ2CT ρD matches that of the buffer within experimental error, Δρd ≈ 0 (see Eq. 1).

To determine if deuterium labeling has caused any changes in the oligmer state and conformation of the proteins and complexes, we have performed light scattering and SAXS experiments to compare the molecular mass and the overall conformation of the deuterated proteins and complexes with their respective unlabeled counterparts in H2O buffer. The labeled dNHERF1 and dPDZ2CT are monomeric similar to the unlabeled proteins (Table I and Figure S1). Comparing the hydrodynamic radii, the radii of gyration and P(r) indicates that deuteration does not cause detectable changes in the overall conformations in dNHERF1 or dPDZ2CT (Table I and Figure 2A and 2C).

The dNHERF1·FERM and dPDZ2CT·FERM complexes are formed by mixing the labeled dNHERF1 with unlabeled FERM, and dPDZ2CT with FERM at 1:1 molar ratio, respectively. The formed complexes are further purified by size-exclusion chromatography. The SLS determined molecular weight indicates that the stoichiometry of the dNHERF1·FERM or the dPDZ2CT complex is 1:1 (Table I). This result is consistent with our previous surface plasmon resonance binding, static light scattering, and analytical ultracentrifugation results on the unlabeled NHERF1·FERM and PDZ2CT·FERM complexes 31.

Figure 2B, 2D and 2E compares the SAXS data I(Q) and P(r) of the deuterated complexes with the unlabeled complexes. Both the light scattering and SAXS results show that deuterium labeling has no apparent effects on the global conformation and the oligomer state of the complexes at the resolution of the scattering experiments, although there is a report from high-resolution crystallography studies that per-deuteration can cause subtle structural changes at the catalytic sites of haloalkane dehalogenase and alters the catalytic functions of this enzyme 45.

At various D2O concentrations, the oligomer state and stoichiometry of the dNHERF·FERM and dPDZ2CT·FERM complexes can also be determined by contrast variation SANS. Figure 3 is a plot of the normalized square-root of the forward scattering intensity I(0), which is the neutron scattering intensity extrapolated to Q=0 A-1, against the scattering length density of the buffer ρo. Because the reduced SANS intensity are on absolute scales, Figure 3 yields an estimate of the molecular volume or the molecular mass of the measured complex, according to Eq. 2 derived from a formula described by Jacrot and Zaccai 46:

Figure 3
Using contrast variation SANS to determine the stoichiometry of the complexes. [I(0)/N]0.5 vs. ρo plots of the dNHERF1·FERM (filled square) and dPDZ2CT·FERM (filled circle) complexes. The forward neutron scattering intensity I(0) ...
[I(0)N]0.5=[(ρDρo)VD+(ρHρo)VH]=υ¯NA[ρDMD+ρHMHρo(MD+MH)]
(2)

where N is the number of the complexes in a volume of 1 cm3, VH=MHυ¯HNA and VD=MDυ¯DNA are the molecular volume of the hydrogenated component and that of the deuterium labeled component, respectively. MH and MD are the molecular masses of the hydrogenated and deuterium labeled components, respectively, and [upsilon]H[upsilon]D[upsilon] is the partial specific volume. According to Eq (2), the slope of a linear fit to the normalized I(0)0.5 vs. ρo plot gives the molecular volume or the molecular mass, and thus the stoichiometry of the complexes.

Linear fits to the [I(0)/N]0.5 vs. ρo plots in Figure 3 give the molecular volume of 75207±2655 Å3 for the dPDZ2CT·FERM complex, and 93891±1098 Å3 for the dNHERF1·FERM complex. Assuming the partial specific volume of the complexes is 0.75 cm3/g, the slopes of Figure 3 give the molecular mass of dPDZ2CT·FERM to be 60.4±8.0 kDa, and that of dNHERF1·FERM to be 75.4±9.6 kDa, which are close to the calculated molecular masses of a 1:1 dPDZ2CT·FERM and dNHERF1·FERM complexes, respectively. Contrast variation SANS thus confirms the 1:1 stoichiometry of the dNHERF·FERM and dPDZ2·FERM complexes in D2O buffer solution. Figure 3 also suggests that changing D2O concentrations does not affect the association state of the complex.

At the 42% D2O match point, structural information about the dNHERF1 or the dPDZ2CT component in the complex can be obtained from SANS data (Eq. 1). In 42% D2O buffer solution, P(r) of dPDZ2CT in complex with the FERM domain of ezrin is very different from that of PDZ2CT or dPDZ2CT in solution (Figures 4C and 4D). The conformation of PDZ2CT or dPDZ2CT in solution is more compact than that of dPDZ2CT in the complex. P(r) of dPDZ2CT in the complex resembles that of an elongated rod-like object 42. The maximum dimension Dmax of dPDZ2CT has changed from 85 Å in solution to 125Å in the complex, (Figure 4D). The Rg of dPDZ2CT in the complex is 37.3 ± 1.0 Å, much larger than Rg=28.0±0.6 Å of PDZ2CT or dPDZ2CT in solution.

Comparing the 3-D image of dPDZ2CT in the complex and that of PDZ2CT in solution reveals changes in domain-domain distance between PDZ2 and the EB binding domain (Figure 5C and 5D). In PDZ2CT, the center-of-mass distance between PDZ2 and CT is 45.3 Å (Fig 5C). When bound to the FERM, the center-of-mass distance between PDZ2 and the EB domain is expanded to 80 Å as measured from the 3-D map (Figure 5D). FERM binding thus induces large conformational changes in dPDZ2CT. In particular, the region linking PDZ2 and EB domain becomes extended when EB is bound to FERM.

Conformational changes in the full-length dNHERF1 are significant when in complex with FERM. P(r) of dNHERF1 in the complex is apparently very different from that of NHERF1 or dNHERF1 in solution (Figure 4B). The Rg of dNHERF1 changes from 41.0±1.0 Å in solution to 45.8± 0.8 Å in the complex, but the change in Dmax is less dramatic from 140 Å to 145 Å. Comparing the 3-D shape of dNHERF1 in solution and in the complex shows that the region linking PDZ2 and CT becomes more extended in dNHERF1 (Figure 5A and 5B). The center-of-mass distance between PDZ2 and the EB domain changes from 45.8 Å in solution to 63 Å in the complex, suggesting that domain-domain contacts between PDZ2 and CT are disrupted. In addition, an angle of about 120° is formed between PDZ2 and CT at the location of PDZ2 in the 3-D map of dNHERF1, which is consistent with the shape changes of dNHERF1 P(r) in the FERM bound complex.

A comparison of the 3-D image of dNHERF1 in the complex with that of dNHERF1 in solution indicates that the region linking PDZ1 and PDZ2 also becomes notably more extended upon binding to FERM (Figure 5A and 5B). The center-of-mass distance between PDZ1 and PDZ2 expanded from 57.1 Å in NHERF1 to 67.0 Å in dNHERF1 in the complex. FERM binding at the C-terminal EB domain of NHERF1 thus triggers large conformational changes in domain-domain interactions between PDZ2 and EB, as well as between PDZ1 and PDZ2 at a long 120 Å distance away from the FERM binding site.

The overall conformation of FERM in the complex is similar to the crystal structure

In 100% D2O solution, the neutron scattering length density contrast between dNHERF1 or dPDZ2CT and the buffer becomes zero within experimental error, structural information about the FERM can be obtained. The scattering intensity I(Q) and the overall shapes of P(r) of FERM in both dNHERF1·FERM and dPDZ2CT·FERM complexes agree well with that calculated from the crystal structure of ezrin FERM domain (Protein Data Bank: 1NI2.pdb) 47 (Figure 6). P(r) of FERM measured by SANS also overlies well with that calculated from the crystal structure of the homologous radixin FERM domain that is in complex with a 13 amino acid C-terminal tail peptide of NHERF1 reported 35. The neutron scattering results suggest that the overall structure of FERM domain is not changed upon forming complex with NHERF1 or with PDZ2CT.

Figure 6
Comparing the conformation of FERM in the complexes with the crystal structure. (A) SANS data of the FERM domain of ezrin in complex with dPDZ2CT (filled circle) and with dNHERF1 (filled square) at the 100% D2O contrast match point. The line is the fit ...

The 3-D envelope of FERM was reconstructed from the SANS data at the contrast match point of dNHERF1 in 100% D2O buffer (Figure 6C). Figure 6C also shows the rigid-body docking of the crystal structure of FERM to the 3-D envelope reconstructed from SANS. The SANS experiments thus reveal that, at the 100% D2O contrast match point, binding to NHERF1 does not cause large scale conformational changes or domain rearrangement in FERM. In the crystal structure of radixin FERM bound to carboxy-terminal peptide NHERF1, the carboxy-terminal peptide of NHERF1 induces conformational changes in the C subdomain of radixin FERM35. These subtle movements of secondary structures in the subdomain C are not apparent at the resolution of the SANS experiments.

Contrast variation SANS reveals the composite structures of the dNHERF1·FERM and dPDZ2CT·FERM complexes

To determine the composite structure of the dNHERF1·FERM and the dPDZ2CT·FERM complexes, we have employed the two phase ab initio method to reconstruct the 3-D images of the dNHERF1·FERM and the dPDZ2CT·FERM complexes using the SANS data at various contrasts. The 3-D images of the dNHERF1·FERM and dPDZ2CT·FERM complexes were restored using MONSA by fitting the multiple neutron scattering curves simultaneously at various contrasts 38; 48 (Figure 7). In the complex composed of deuterium labeled and unlabeled components, the deuterated component represents one phase, and the unlabeled component is designated as the 2nd phase.

Figure 7Figure 7
Contrast variation SANS reveals the composite structure of the dNHERF1·FERM complex. (A) SANS data of dNHERF1·FERM (left panel) and dPDZ2CT·FERM (right panel) at various contrasts. The lines are fits to the scattering curves when ...

Figure 7C is the composite structure of the dNHERF1·FERM complex, which shows the 3-D shape and the spatial orientation of the deuterium labeled dNHERF1 and the unlabeled FERM components. Although the reconstructed 3-D models of the dNHERF1·FERM complex and its two components are somewhat less detailed than the individual components reconstructed at contrast match points, the size and shape of dNHERF1 in the composite structure are very similar to that reconstructed at the 42% D2O match point, and the compact conformation of FERM in the complex is compact similar to that reconstructed at the 100% D2O match point.

The composite structure of the dNHERF1·FERM complex reveals the relative orientation and position of the two components, with the FERM domain located at one end of dNHERF1. The PDZ domains and the FERM domain are assigned in the composite structure. The composite 3-D shape of the dPDZ2CT·FERM complex (Figure 7C right panel) has a compact phase that resembles the shape of FERM domain, and an elongated phase that represents the opened dPDZ2CT (Figure 7C). Upon binding to FERM, dPDZ2CT becomes extended. The FERM domain is bound at one end of PDZ2CT, presumably at the EB domain.

Discussion

Using solution small angle X-ray scattering and contrast variation small angle neutron scattering, we show that binding of the FERM domain of ezrin to the C-terminus of scaffolding protein NHERF1 induces large conformational changes in NHERF1. Large conformational changes occur in the region linking PDZ2 and the ezrin-binding domain, suggesting that intramolecular domain-domain interactions between PDZ2 and the EBD are disrupted upon ezrin binding. Significant conformational changes are also apparent in the region that links PDZ1 and PDZ2. The present neutron scattering results thus provide a structural explanation of our binding results and thermodynamic analyses, which demonstrate positive allosteric regulation of NHERF1 by ezrin as it assembles membrane protein complexes. The neutron scattering results reveal that this allosteric control originates from domain-domain interactions.

Our binding and thermodynamic analyses show PDZ1, PDZ2 and EBD are energetically coupled, see Figure 1. FERM binding to the C-terminus of NHERF1 increases the binding capability of both PDZ2 and PDZ1. The SAXS and SANS data show that NHERF1 adopts an elongated conformation, with PDZ1, PDZ2 and the C-terminal domain arranged in a row, see Figure 2. The center-of-mass distance between PDZ1 and EBD are 120 Å apart. Thus, together with the thermodynamic analyses, the results demonstrate that all three distant binding sites, PDZ1, PDZ2, and EBD communicate with each other at a very long distance.

What is the physiological consequence of allosteric regulation of NHERF1 by ezrin as it assembles membrane proteins? Studies of the intracellular dynamics of ezrin and ion transport proteins provided the first clues. In cells, the internalization of membrane of transporters and/or receptors, and the recycling of these membrane proteins back to the cell surface are dynamic processes 25; 49; 50; 51; 52. PDZ proteins and possibly the activation of ezrin play a significant role in the retaining transport proteins or receptors at the membrane. Ion channels and/or receptors proteins complexed with NHERF1 and activated ezrin are populated in the more immbile apical membrane subdomains because of their interactions with actin cytoskeleton. The inactive ezrin is localized in the more mobile endosomes that are exchanging with the immobile apical subpopulaltion as well as with late endosomes. Allosteric control of NHERF1 by activated ezrin to assemble protein complexes may be an important mechanism for engaging cross-talk among ion transport proteins and/or receptors while they reside in the cell membrane, thus modulating the strength and magnitude of signaling. Allosteric regulation of NHERF may also be an effective means to terminate signaling once ezrin is deactivated and the membrane protein is internalized, thus control the duration of signaling. The actin cytoskeleton thus dictates a control of membrane receptors and/or ion transport proteins via the allosteric regulation of NHERF1 by ezrin. Future studies of how the conformational dynamics of ezrin and NHERF1 and the actin cytoskeleton influence the signaling of ion transport proteins or receptors in cellular context will provide more evidence of the physiological significance of allosteric regulation of NHERF1 by ezrin and the cytoskeleton.

What is the mechanism of FERM induced long-range conformational changes and long-range energetic coupling in NHERF1? A type I PDZ-binding motif -SNL at the carboxy-terminus of NHERF1, which overlaps with the EB sequence, has been proposed to interact with PDZ2 through intramolecular domain-domain interactions and hold PDZ2 in an auto-inhibition state 41; 53. Our NMR studies indeed find that there are dynamic intramolecular interactions between the NHERF carboxy-terminus and the carboxy-binding loop and the peptide binding pocket in PDZ2, although the intramolecular interactions are quite weak (Bhattacharya et al, submitted). The NMR studies also show that the CT domain, including the EB domain and the region linking PDZ2 and the EB domains, is largely disordered. Because FERM interacts with the EB domain strongly, FERM binding can easily overcome the relatively weak intramolecular interactions between EBD and PDZ2. The crystal structure shows that the NHERF1 C-terminal (348-358) adopts α-halical conformation when bound to FERM. Combining the NMR, crystallography and the present SANS studies suggests that FERM binding disrupts the intramolecular interactions between PDZ2 and EB, and uncovers PDZ2 to have increased binding affinity for target proteins.

Moreover, there is also evidence that PDZ1 interacts with the linker region between PDZ1 and PDZ232. The binding affinity of PDZ1 for its target proteins is increased when including a flank region at the carboxy-terminal end beyond the canonically identified PDZ structural boundary (ref. 54 and Li et al in preparation). The conformation of the PDZ1-PDZ2 linker region thus affects the binding affinity of PDZ1. Ezrin binding may trigger conformational changes in PDZ2, and PDZ2 in turn propagates the allosteric signals 55; 56 to cause conformational changes in the PDZ1-PDZ2 linker region. As a result, binding affinity of PDZ1 for its target proteins is increased at a site far away from the ezrin binding site. Our SAXS and SANS studies of the conformational changes in NHERF1 complexes are on mesoscopic scales. High resolution structural studies of the conformational changes in the PDZ1 plus the linker region will provide insight into the mechanism of such allosteric domain-domain interactions at atomic resolution.

There is increasing evidence that fast protein dynamics dictate energy propagation and the allosteric behavior in a protein 55; 56; 57; 58; 59; 60; 61. Binding inspires changes in deterministic motion on fast ps to ns time scales, which then facilitates the stochastic motion needed to make large conformational changes longer micro to millisecond time scales. In the case of NHERF1, protein motion may play significant roles in influencing the long-range allosteric behavior not only within a PDZ domain 55; 56; 62 but also at the domain level. It would be interesting to determine the time-scale and amplitude of protein motion within a domain as well as long-range coupled protein domain motion 63; 64; 65 in NHERF1, and estimate how protein domain motion affects the allosteric binding properties of NHERF1.

Materials and Methods

Protein expression and purification

The pET151/D-TOPO vector (Invitrogen, Inc) was used to express the FERM domain of human ezrin (FERM, amino acid residues 1-298), the full-length human NHERF1 (residues 11-358), and PDZ2 plus the C-terminal domain (PDZ2CT, residues 150-358). The protein expressed by the pET151/D-TOPO vector contains an N-terminal V5 epitope plus a hexa-histidine fusion tag. All plasmids were subjected to DNA sequencing to verify the DNA sequence.

The protein expression plasmids were transformed into Rosetta 2(DE3) cells (Novagen). The cells were grown until the optical density at 600 nm reaches 0.8-0.9, and were induced with 0.5 mM Isopropyl β-D-1-thiogalactopyranoside (IPTG) for 2 hours. The harvested cells were resuspended and lysed in buffer containing 20 mM sodium phosphate buffer, 150 mM NaCl, 0.1 mM phenylmethylsulfonyl fluoride (PMSF), and 10 mM imidazole, pH=7.5. The protein extracts were first further purified by Ni2+ HiTrap chelating column (Amersham Biosciences). The proteins were then purified and analyzed by size-exclusion chromatography, using a Superdex 200 10/30 column (Amersham Biosciences). The N-terminal fusion tag was cleaved using Tobacco Etch Virus protease (Invitrogen) after purification. After fusion tag cleavage, the calculate molecular masses for 38656 g/mol for NHERF1, 23505 g/mol for PDZ2CT, and 35772 g/mol for FERM.

When expressing deuterated full-length NHERF1 and PDZ2CT, Rosetta 2 (DE3) cells were grown at 37°C in sterile M9 medium containing 99.9% D2O (Cambridge Isotope Laboratories) until O.D.600nm reaches 0.8. The cells were induced with 0.25 mM IPTG. Protein purification and fusion tag removal for the deuterated proteins were the same as described above.

Mass spectroscopy experiments for determining deuterium content in labeled proteins

The deuterium content of dNHERF1 and dPDZ2CT was measured by MALDI-TOF usinga Reflex IV mass spectrometer (Bruker Daltonics, Billerica, MA) between 5000 and 60,000 m/z in linear mode. The protein peaks were internally calibrated using myoglobin as standard. The non-exchangeable deuterium content determined by mass spectroscopy is 67% for dNHERF1, and 73% for dPDZ2CT. The molecular mass of dPDZ2CT is 24691 g/mol, and that of dNHERF is 40017 g/mol.

Static and dynamic light scattering experiments

Dynamic light scattering experiments were performed using a DynaPro Molecular Sizing Instrument (Wyatt Technology Corporation) with a laser of wavelength 824.7 nm at a fixed 90° scattering angle. Before light scattering experiments, the sample was centrifuged at 10,000 rpm for 5-10 minutes. Protein concentrations were varied from 0.5-2 mg/ml during light scattering measurements. Light scattering experiments were performed at 10°C.

Small angle X-ray and neutron scattering experiments

The SAXS instrument and data reduction methodology have been described previously 31; 41; 66; 67 In the present study, a typical 0.01 <Q< 0.3 Å-1 range is employed, where Q=4πsin(θ/2)λ is the magnitude of the scattering vector, θ is the scattering angle, and λ=1.54 Å-1 is the wavelength of the Cu-Kα X-ray.

The protein concentrations used for SAXS experiments are about 1-2 mg/ml, at which, NHERF1, PDZ2CT, and the dNHERF1·ezFERM and dPDZ2CT·ezFERM complexes are monomeric. At these concentrations, the inter-molecular interference effects are negligible because the radii of gyration Rg and the protein concentration normalized forward scattering intensities I(0) are independent of protein concentrations. SAXS experiments were performed at 10°C.

Small angle neutron scattering experiments were performed at the NG7 30m SANS instrument at the NIST Center for Neutron Research, National Institute of Standards and Technology 68. The neutron wavelength was 6 Å with the neutron wavelength spread (Δλ/λ) being 0.11 (full width at half-maximum). The source aperture diameter was 5.08 cm, and the source-to-sample length was 542 cm. The data were collected at two sample-to-detector distances, 265 and 125 cm, and both sample-to-detector distances have a 25 cm detector offset. These settings gave the effective Q range between 0.0122 and 0.4596 Å-1.

For contrast variation SANS experiments, the dPDZ2·ezFERM and dNHERF1·ezFERM complexes were exchanged, respectively, into 0%, 10, 20%, 42%, 80%, 90% and 100% volume fractions of D2O buffers containing 20 mM Tris-HCl (pH=7.5) and 150 mM NaCl. The concentration of the dPDZ2CT·ezFERM complex was 3.72 mg/ml, and the concentration of dNHERF1·ezFERM was maintained at 2.02 mg/ml in H2O solution and in all different volume fractions of D2O solutions. For protein samples dissolved in 100%, 90% and 80% D2O solution, 2 mm path-length quartz cells were used. For samples dissolved in 0%, 10%, 20% and 42% D2O solutions, 1 mm path-length quartz cells were used. SANS measurements were performed at 10°C.

SANS data were reduced using the procedures developed at NIST 69. The two-dimensional scattering data were corrected for nonuniform detector response, and for scattering from the quartz cells and from ambient neutrons in the room. The scattering data were normalized by the incident beam flux on the sample and by sample thickness to obtain absolute scale intensity (scattering cross section per unit volume). The corrected data were then circularly averaged to yield the one-dimensional I(Q). The incoherent background scattering was determined by averaging the intensity of the plateau region of Q between 0.35 to 0.45 Å-1, and subtracted from the scattering data.

The reduced scattering data are plotted as scattering intensity I(Q) vs. Q profiles. Inverse Fourier transformation of I(Q) gives the length distribution function P(r) that is the probability of finding two scattering points at a given distance r from each other in the measured macromolecule. P(r) was generated by the program GNOM 70. The radius of gyration Rg, which is related to the size and shape of a protein, can be obtained from P(r) or from the Guinier approximation 42.

The 3-D molecular envelopes were reconstructed from SAXS or SANS using the ab intio program DAMMIN developed by Svergun et al 48 to generate a set of PDB-formatted dummy bead coordinates. For each construct, about 10-15 models were generated by running the program in slow mode or expert mode. The models are averaged using DAMAVER 71. The normalized spatial discrepancy (NSD) values, which measure the reproducibility of the models used in averaging, are given in the figure legends. The program package Situs was used to convert the dummy bead coordinates into envelopes 72. Situs was also used for rigid-body docking of the crystal structure to the generated envelopes. The graphics are generated using UCSF Chimera 73.

Supplementary Material

Acknowledgments

This work utilized facilities supported in part by the National Science Foundation under Agreement No. DMR-0454672. We acknowledge the support of the National Institute of Standards and Technology, U.S. Department of Commerce, in providing the neutron research facilities used in this work. We thank the Biotechnology Facility at the Fox Chase Cancer Center for mass spectrometry support. We thank Paul Butler and Susan Krueger for helpful discussion. This work is supported by the National Institutes of Health Grant R01 HL086496 and W.W. Smith Charitable Trust (to Z. B.).

Footnotes

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