PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Expert Opin Emerg Drugs. Author manuscript; available in PMC 2010 June 1.
Published in final edited form as:
PMCID: PMC2756337
NIHMSID: NIHMS125373

Atrial-selective sodium channel block for the treatment of atrial fibrillation

Alexander Burashnikov, PhD1 and Charles Antzelevitcht, PhD, FACC, FAHA, FHRScorresponding author2

Abstract

The pharmacological approach to therapy of atrial fibrillation (AF) is often associated with adverse effects resulting in the development of ventricular arrhythmias. As a consequence, much of the focus in recent years has been on development of atrial-selective agents. Atrial-selective sodium channel blockers have recently been shown to exist and be useful in the management of AF. This review summarizes the available data relative to current therapies, focusing on our understanding of the actions of atrial selective sodium channel blockers in suppressing and preventing the induction of AF and electrophysiological properties that confer atrial-selectivity to these antifibrillatory drugs.

Keywords: arrhythmias, electrophysiology, pharmacology, sodium channel blocker

1. Background

Atrial fibrillation (AF) is the most prevalent sustained clinical arrhythmia associated with increased morbidity and mortality. The prevalence of AF is 0.4 – 1% in the general population and greater than 8% in individuals > 80 years of age. Approximately 2.3 million individuals in the North America and 4.5 million in Europe are affected by AF [1,2]. These numbers are projected to increase to 15 million in North America alone by 2050 [2] due largely to aging of the population. Although AF can occur in people with undetectable heart diseases (the so-called ‘lone or idiopathic AF’), this arrhythmia is normally associated with a number of clinical, often overlapping, conditions such as hypertension, heart failure, coronary artery disease and valvular heart disease, with an incidence of 80, 30, 25 and 17% in AF patients, respectively [2]. In terms of duration, AF is commonly defined as paroxysmal, persistent (lasting < 7 and ≥ 7 days, respectively), and permanent AF (which cannot be terminated or is not attempted to be terminated) [3]. AF is a progressive disease. If not treated appropriately, paroxysmal AF tends to recur progressively more often, last longer and transition to a persistent or permanent form. It is now well established that the longer the duration of AF, the more difficult it is to suppress the arrhythmia. The development of electrical and structural remodeling secondary to AF contributes to the tendency of AF to progress from paroxysmal to persistent and permanent. This progression has led to coining of the phrase “AF-begets AF” by Allessie’s group in 1995 [4]. Another contributing factor is structural remodeling of the atria associated with aging, hypertension and heart failure.

Despite significant progress in non-pharmacological treatment of AF (largely due to the use of catheter ablation techniques), antiarrhythmic drugs (AADs) remain the first-line therapy for rhythm control of AF [1,3]. However, the efficacy and/or safety of AADs available for the treatment of AF are not optimal. Recent drug development has focused on the atrial-selective anti-AF medications, with the goal of avoiding the ventricular proarrhythmic effects of at present available drugs. We recently introduced the concept of atrial-selective sodium channel blockers as a novel strategy for the management of AF [5]. This review summarizes available data relative to current therapies and our understanding of the actions of atrial-selective sodium channel blockers in suppressing and preventing the induction of AF, as well as the biophysical properties that confer atrial-selectivity to AADs.

2. Unmet medical need and existing treatment

Effective and safe treatment of AF remains a major unmet medical need in our society. There are two principal options for the management of AF, that is, rate and rhythm control. Rate control, in which the atria is left fibrillating, is focused on reducing the number of beats crossing the atrioventricular node and thus preventing the detrimental effects on the ventricles, such as the development of cardiomyopathy and heart failure. The rhythm control strategy aims to maintain sinus rhythm, with its restoration when required (using AADs, catheter ablation or surgical techniques). Rate control and most rhythm control patients require anticoagulation therapy to reduce the risk of stroke [2,6].

Clinically available rhythm control anti-AF agents target the rapidly activating delayed rectifier potassium current (IKr, e.g., dofetilide and sotalol), the ‘early’ or ‘fast’ sodium current (INa, e.g., flecainide and propafenone) or several ion channels (potassium, sodium and calcium; e.g., amiodarone and dronedarone). The ability of AADs (both INa and IKr blockers) to acutely restore sinus rhythm is generally better with paroxysmal than persistent AF (ranging from 30 to 90% and 6 to 30% of AF patients, respectively) [2,6]. IKr blockade is generally more effective than INa blockade in the termination of persistent AF [1]. The efficacy of long-term maintenance of sinus rhythm (at 1 year) ranges between 40 and 50% with IKr and INa blockers and is up to 65% with amiodarone, perhaps the best available antiarrhythmic agent for the maintenance of sinus rhythm [6]. Unfortunately, the use of all currently available AADs is associated with a risk of induction of ventricular arrhythmias and/or organ toxicity. IKr blockers may induce acquired long QT syndrome and polymorphic ventricular tachycardia, known as Torsade de Pointes (TdP) [1]. Before 1989, agents that blocked INa were considered to be rather safe and were widely used for the treatment of both atrial and ventricular arrhythmias. In 1989, the Cardiac Arrhythmia Suppression Trail (CAST) revealed a shocking reality that potent INa blockers (Class IC) significantly increase total mortality in patients with previous myocardial infarction, presumably owing to severe ventricular arrhythmias [7]. As a consequence, the use of INa blockers for ventricular arrhythmias was significantly reduced and the development of new antiarrhythmic INa blockers was practically stopped. In contrast to ventricular arrhythmias, potent INa blockers such as flecainide and propafenone have been widely used before and after the CAST era for rhythm control of paroxysmal AF, with an important CAST-derived limitation of not using these agents in patients with structural heart diseases such as congestive heart failure, myocardial infarction or hypotrophy: these account for > 70% of all AF patients [2,6]. Although amiodarone is safe for use in patients with structurally-compromised ventricles, long-term administration of this agent can induce multi-organ toxicity and rare cases of TdP [8,9].

A number of recent randomized, prospective and multi-center studies have provided compelling evidence that catheter ablation strategy, primarily targeting pulmonary vein regions of the left atrium, is more effective than AADs in the long-term maintenance of sinus rhythm in symptomatic and relatively young patients with paroxysmal AF in which at least one AAD had initially failed [1012]. The long-term efficacy of catheter ablation for the treatment of persistent/permanent AF is generally lower than that of paroxysmal AF [3]. It is yet to be determined if rhythm control strategy based on catheter ablation method is superior to that based on AAD approach in terms of total mortality [2,13]. Notwithstanding the increasing efficacy of catheter ablation and the limitations of use of AADs, at the present time AADs remain the first-line therapy for rhythm control management of all types of AF patients, including symptomatic and relatively young patients with paroxysmal AF [13,6].

The results of a number of multi-center, randomized and prospective clinical trails (such as AFFIRM and AF-CHF) suggest that rhythm control strategy with AADs is not superior to rate control in terms of survival [14,15], A common interpretation of these data is that the adverse effects of using AADs, secondary to extra-cardiac toxicity and ventricular proarrhythmia, exceed the benefit derived from a limited capability of these AADs to maintain sinus rhythm [2,6,16]. The maintenance of sinus rhythm with AADs was not achieved in all rhythm control patients and not all rate control patients were in AF all the time. When these considerations were taken into account, several post hoc analyses of the AFFIRM data showed that patients who were maintained in sinus rhythm had a better survival rate than those who were in AF [1719]. It is generally believed that rhythm control with AADs would be preferable for most AF patients if safer and more effective anti-AF drugs were available [2,6,20].

Several novel pharmacological strategies aimed at improving the effectiveness and safety of AADs for AF rhythm control management have been proposed [2022]. “Upstream therapy” is focused largely on non-ion channel targets designed to reduce and/or prevent atrial structural remodeling, hypertrophy, dilatation, hypertension, inflammation, oxidative injury and so on (i.e., factors believed to be involved in the generation of AF) [20,23]. “Upstream therapy” with angiotensin-converting enzyme inhibitors, angiotensin receptors blockers, statins, aldosterone antagonists and (ω-3 poly-unsaturated fatty acids has been shown to be of benefit for AF in some but not all studies [24,25].

Recent years have witnessed the development of novel ion-channel blockers that are atrial specific/selective in their actions as well as efforts to improve old anti-AF agents (e.g., dronedarone to replace amiodarone). Atrial-specific targets are those that are present exclusively or almost exclusively in atria and include the ultrarapid delayed rectified potassium current (IKur) the acetylcholine (Ach)-regulated inward rectifying potassium current (IK-ACh), the constitutively active (CA) IK-ACh (i.e., which does not require ACh or muscarinic receptors for activation) and connexin 40 (with a condition that connexin 40 is present in ventricular conduction system) [21,26,27]. Inhibition of both the conventional IKACh and CA-IKACh has been shown to exert antiarrhythmic actions in some types of experimental AF [28,29]. Gap junction activator rotigaptide produces antiarrhythmic actions in canine chronic mitral regurgitation AF model [30] and in the canine acute ischemia AF model [31] but did not affect AF development in AF models associated with heart failure or atrial tachypacing [30,31]. The clinical applicability of inhibitors of conventional and CA-IKACh as well as enhancers of gap junctional conductance remains to be determined [27].

Among the atrial-specific approaches, the inhibition of IKur is the most investigated and widely considered to be the most promising for the treatment of AF [21,32]. However, at concentrations that effectively control AF, IKur blockers also potently block other currents (e.g., INa by vernakalant and AZD7009 and Ito/IKACh/CA-IKACh by AVE0118) [3336]. Although there are a number of other IKur blockers that have been shown to suppress experimental AF [32], it is yet to be proven that pure IKur block can effectively suppress AF (see section 4.1). Selective IKur inhibition can neither prevent nor terminate ACh-mediated AF in canine atria [37]. It has been reported that IKur density is progressively reduced with acceleration of activation rates [38] and that IKur density can be decreased in cells isolated from chronic AF atria [36,39]. It has been shown also that selective IKur reduction produces only minor (action potential duration) APD90 prolongation in human remodeled atria or canine ACh-treated atria (both showing a triangular action potential morphology and prone to develop AF) [37,40]. These data indicate that the relative contribution of IKur to atrial repolarization in remodeled atria may be relatively low, particularly at rapid activation rates. Although IKur block may contribute to the antiarrhythmic efficacy of the IKur blockers, IKur block alone may be insufficient to effectively suppress AF and inhibition of further currents may be required (e.g., INa, IKr, Ito, IKACh, CA-IKACh) [22,27,36,37,41]. In addition, IKur may actually predispose to the development of AF in non-remodeled atria [37,42]. In human and canine non-remodeled atria (displaying a plateau-shaped action potential morphology), selective IKur block abbreviates APD90 [37,40,43], a well-recognized pro-AF factor [44], and permits the development of AF in ‘healthy’ canine atria [37]. Consistent with these data, loss-of-function mutations in KCNA5, the gene that encodes the KV1.5 channels responsible for IKur, was found to be associated with AF [42]. Note, however, that only one AF patient with the KCNA5 mutation has been identified thus far.

Recent experimental studies have identified atrial-selective or predominant INa blockers that can effectively suppress AF with little or no effect in the ventricles [5,4547]. Based on these proof-of-concept experiments, we have proposed a novel investigational atrial selective sodium channel block strategy for AF treatment. We refer to atrial-selective or predominant targets as those that are present in both chambers of the heart, but whose inhibition produces greater effects in atria versus ventricles. In these cases, block of INa leads to atrial-selective depression of excitability and conduction as well as the development of post-repolarization refractoriness (PRR) in multicellular cardiac preparations or in vivo.

3. Current research goals

Data relative to atrial-selective sodium channel block as a strategy for the management of AF appeared only in 2007 [5], and so a number of critical questions remain to be answered. Among these are: What properties of sodium channel blockers (kinetics and modes of binding and unbinding, lipid solubility, molecular size and chemical structure) determine their atrial selectivity? How does electrical and structural remodeling in both atria and ventricles modify atrial selectivity and anti-AF potential of INa blockers? What factors/conditions may promote atrial selectivity and anti-AF efficacy of sodium channel blockers? What specific types of AF pathologies can be treated most effectively and safely with atrial selective INa blockers? Are acute and chronic effects of atrial selective INa blockers on the heart similar? Are atrial selective INa blockers safe in the short and long term, particularly in structurally compromised hearts (both in terms of proarrhythmia and extra-cardiac toxicity)? To what extent can data on atrial selectivity of INa blockers obtained from dog be applied to humans? What is the genetic basis for the atrioventricular difference in biophysical properties of sodium channel and how do these differences in biophysical properties contribute to atrioventricular distinctions in pharmacologic response? Can these genetic differences be applied to the development of atrial-specific treatments of AF? Current research is focused on finding answers to these and related questions. The remainder of the review attempts to summarize the available information regarding these issues.

4. Scientific rationale

4.1 Atrial-selective depression of sodium channel-mediated parameters

The emergence of the atrial selective sodium channel block as a strategy for AF therapy derives from experimental findings that ranolazine predominantly depresses atrial versus ventricular sodium channel-dependent parameters and suppresses AF at concentrations producing little to no effect in the ventricles (Figures 1 and and2)2) [5]. Similar atrial selective properties were revealed later with chronic amiodarone [45]. Ranolazine and chronic amiodarone reduce maximum rate of rise of the action potential upstroke (Vmax), prolong conduction time (CT), increase diastolic threshold of excitation (DTE) and induce PRR specifically or predominantly in the canine isolated atrial versus ventricular coronary-perfused preparations (Figures 1 and and3)3) [5,45]. Induction of PRR is a unique feature of INa blockers, occurring when the effective refractory period (ERP) is prolonged without prolongation of action potential duration (APD70–90) or to a greater degree than APD70–90. Lidocaine exerts mild atrial-predominant effects on these INa-mediated parameters, whereas ranolazine and chronic amiodarone are strongly atrial-selective in their suppression of INa-dependent parameters [5,45]. In contrast, propafenone depresses VMax and CT, decreases DTE and induces PRR in a chamber-independent manner at a pacing cycle length of 500 ms, but become a bit more atrial-selective at a basic cycle length of 300 ms (see Section 4.2) [48].

Figure 1
Ranolazine specifically induces prolongation of the ERP and development of post-repolarization refractoriness in atria (the difference between ERP and APD75 in atria and between ERP and APD90 in ventricles; ERP corresponds to APD75 in atria and APD90 ...
Figure 2
Ranolazine suppresses AF and/or prevents its induction in two experimental models involving isolated arterially-perfused right atria at concentrations producing little to no effects in ventricles
Figure 3
Ranolazine produces a much greater rate-dependent inhibition of the maximal action potential upstroke velocity (Vmax) in atria than in ventricles

Interestingly, although lidocaine is a specific INa blocker, ranolazine and amiodarone are not. Ranolazine, first recognized as an antianginal and then as an antiarrhythmic agent, in therapeutically relevant concentrations (1 – 10.0 μM) blocks early INa, late INa, IKr and late ICa [5,49]. Amiodarone has likewise been shown to inhibit several cardiac ionic currents (IKr, IKS, INa, late INa, Ito ICa–L, ICa–T IK1 IK(ACh) and IK(ATP) as well as to block α- and (β-adrenoceptors [8,50].

AZD7009 decreases excitability (i.e., DTE) and conduction velocity preferentially in atria of dogs in vivo [51], indicating that its atrial selectivity is due in part to its inhibition of INa giving rise to an atrial selective prolongation of ERP [34,51].

A prominent IKur blocker, AVE0118, also inhibits INa (decreases VMax) in an atrial selective manner and its atrial selectivity in ERP in non-remodeled atria is owing to sodium channel inhibition [52]. This atrial selectivity of AVE0118 and its INa blocking potency is weaker compared to ranolazine. Vernakalant, another IKul blocker, also potently blocks INa [33]. ISQ-1 and TAEA, two more IKur blockers, slow conduction velocity in atria but not in ventricles [53], suggesting an ability to block INa in an atrial selective manner. Interestingly, in non-remodeled atria, IKur blockers abbreviate or produce no change in APD70–90 [37,40,43,54], but apparently always prolong ERP in both non-remodeled and remodeled atria [32], which can be explained with the induction of sodium channel-dependent PRR (as has been demonstrated for AVE0118 in canine non-remodeled atria [52]). Of note, the effects of most IKur blockers on INa- or INa-mediated parameters have either not been studied or studied under conditions that may not unmask their atrial selectivity (i.e., in ventricular myocytes at slow pacing rates) [55,56].

Atrioventricular differences of the response to INa blockers are poorly investigated and controversial (for review see [47]). Failure to reveal the atrial selectivity of INa blockers in some studies may be due to the use of superfused atrial and ventricular preparations [34,5761]. In general, whereas ventricular superfused preparations display electrophysiological characteristics and pharmacological responses similar to those of coronary-perfused preparations [5,49], atrial superfused tissue preparations do not [5,43]. Superfused atrial preparations typically display action potentials with a triangular morphology, whereas coronary-perfused atria have action potentials with a prominent plateau [43]. Ventricular action potentials display a plateau in both superfused and coronary-perfused preparations. Atrial APD is much briefer than ventricular APD in superfused preparations, but comparable in coronary-perfused preparations [5,57,58]. Because the efficacy of INa blockers is critically dependent on the duration of the action potential and diastolic interval (with the latter being a function of the former) [62,63], data from superfused atrial preparations should be interpreted with great caution. Semi-quantitative assessment of atrial selectivity of INa blockers is shown in Figure 4.

Figure 4
A semi-quantitative assessment of atrial selectivity of lNa blockers based on studies conducted in atrial and ventricular Cor-perfused and superfused (tissues) preparations, isolated myocytes and in vivo.

4.2 Atrial selective prolongation of APD90 by lKr block promotes atrial selective lNa block

Ranolazine, propafenone and chronic amiodarone (all possessing IKr blocking activity), but not lidocaine, produce a greater prolongation of APD90 in canine atria versus ventricles [5,45,47]. Selective IKr blockers such as E-4031 also preferentially prolong atrial APD90 [45]. These data are consistent with a number of experimental and clinical studies demonstrating a preferential prolongation of ERP in atria versus ventricles by IKr blockers (such as sotalol, d-sotalol, dl-sotalol, dofetilide, WAY-123,398, ibutilide, MK499 and almokalant) at pacing rates equivalent to the resting sinus rhythm [6470]. However, under bradycardic conditions or with long pauses, these agents produce a greater APD prolongation and early afterdepolarizations leading to the development of TdP arrhythmias in the ventricles rather than the atria [71,72]. Rate-dependent atrioventricular differences in response to IKr inhibition are not well appreciated and underlying mechanisms of these differences remain undefined.

The atrial-predominant APD prolongation leads to a greater abbreviation of diastolic interval or its elimination at rapid rates in atria versus ventricles. (Figure 3B) The progressive elimination of the diastolic interval in atria at rapid rates leads to greater accumulation of drug-induced sodium channel block in atria than in ventricles because recovery from sodium channel block generally occurs during the diastolic interval [73]. This mechanism is thought to contribute to greater atrial selectivity of ranolazine and amiodarone versus lidocaine [5]. Thus, IKr inhibition may exert anti-AF activity through a direct effect to prolong APD/ERP as well as by an indirect influence to promote INa blockade in an atrial-predominant manner. The contribution of IKr block to the atrial-selective actions of ranolazine and amiodarone is clearly owing to its potentiation of their action to depress VMax and CT or increase ERP, PRR and DTE through inhibition of sodium channel current. Indeed, ranolazine-induced PRR is much longer than the ranolazine-induced APD75 prolongation (Figure 1).

4.3 Cellular and ionic mechanisms of atrial selectivity of lNa blockers

INa blockers generally bind more effectively to open and/or inactivated sodium channels (i.e., during the action potential) than to resting sodium channels (i.e., during the diastolic interval). Unblocking is largely associated with the resting state of the sodium channel [63,73]. Acceleration of activation rates promotes the development of sodium channel blockade by increasing the proportion of time that the sodium channels are in the open/inactivated state and reducing the time that the channels are in the resting state. There are no ‘pure’ open or inactivated state INa blockers. As a general rule, predominantly inactivated-state blockers are INa blockers having rapid unbinding kinetics (τ ≤ 1 s; i.e., Class IB agents) and predominantly open-state blockers are INa blockers having medium or slow unbinding kinetics (τ > 1 but < 12 s and ≥ 12 s; Class IA and IC agents, respectively) [62,73]. Depolarization of the resting membrane potential (RMP) normally promotes and abbreviation of APD reduces the efficacy of INa block.

Available data suggest that open versus inactivated state block of the sodium channel does not determine the potential for atrial selectivity. Although both propafenone and ranolazine are predominantly open state blockers [63,74] (Nesterenko et al., unpublished), amiodarone and lidocaine are predominantly inactivated state blockers [63]. Rate of dissociation of drug from the sodium channel, on the other hand, is thought to contribute to atrial selectivity. Ranolazine and amiodarone, both atrial-selective sodium channel blockers, possess relatively rapid dissociation kinetics (unbinding τ = 0.2 – 1.6 s) [5,75] whereas propafenone, which shows little to no atrial selectivity, displays slow dissociation kinetics (unbinding τ ≥ 8 s) [63]. Validation of this hypothesis awaits assessment of the atrial selectivity of other ‘slow’ INa.

The ‘atrial-selective’ properties of INa blockers are owing to atrioventricular differences in the biophysical properties of the sodium channel and differences in the morphology of atrial and ventricular action potentials (Figure 5) [5,45,47]. The RMP is intrinsically more depolarized in atrial versus ventricular myocytes, presumably owing to a weaker IK1 in atria [76]. The steady-state inactivation curve of the atrial versus ventricular INa is shifted to the left, with the half inactivation voltage (V0.5) in atrial myocytes being 9–14 mV more negative than that of ventricular myocytes [5,77,78]. As a consequence of the more depolarized RMP and more negative V0.5, a larger fraction of sodium channels are in the inactivated state in atrial versus ventricular cells. The fraction of resting sodium channels is, therefore, smaller in atrial versus ventricular cells at the RMP. Because much of die recovery from sodium channel block commonly occurs during the resting state of the channel [62,73], atrial cells should show a greater accumulation of use-dependent sodium channel block. Atrial-selective APD prolongation (owing to IKr block) may also importantly promote atrial selective depression of sodium channel- dependent parameters (discussed in Section 4.2).

Figure 5
Activation and steady-state inactivation in atrial versus ventricular myocytes

The factors contributing to atrial-selective depression of sodium channel-dependent parameters at rapid activation rates include: i) A more negative half-inactivation voltage in atria, which reduces the availability of sodium channels and the fraction of resting sodium channels; ii) A more depolarized RMP in atria, which further reduces the availability of sodium channel and potentiates the effect of sodium channel blockers; iii) Greater drug-induced slowing of Phase III in atria (owing to IKr block), which results in failure of the action potential to achieve maximum resting potential at rapid rates, thus, leading to a depolarized take-off potential, further reducing the availability of sodium channels (Figure 3); iv) The slower Phase III also leads to elimination of the diastolic interval in atria but not ventricles, thus, reducing the rate of dissociation of sodium blockers from the channel (Figure 3); and v) Recovery from inactivation of the sodium channel is slower in atrial cells [78].

It is noteworthy that INa density is much greater in atrial versus ventricular cells (Figure 5) [5,78]. The higher density of INa in atrial cells [5,78] may offset the lower availability of sodium channels in atrial versus ventricular cells. The time constants for sodium channel activation and inactivation are also twice as rapid in atrial as in ventricular myocytes [78], indicating that the total open time of the sodium channels during each action potential should be shorter in atrial cells.

4.4 Atrial-selective multiple ion channel block for the management of AF with the sodium channel as the prime target

Although the promise of selective ion channel block for the management of AF has been attractive in theory, practical clinical experience and experimental evidence suggest that ‘dirty’ drugs affecting several ion currents, such as amiodarone, are generally more effective. Clinical data indicate that selective INa blockers, such as lidocaine or mexiletine (Class IB agents), which have rapid binding/unbinding kinetics, are not very effective in suppressing AF [1]. All clinically effective anti-AF Class I agents inhibit several currents (such as IKr, IKs, Ito) and have relatively slow binding/unbinding kinetics from the sodium channel (e.g., flecainide or propafenone, Class IC; and quinidine, Class IA). It is not clear whether ‘pure’ INa block, independent of dissociation kinetics, is capable of suppressing clinical AF at therapeutic concentrations of INa blockers. Experimental and theoretical data have shown that ‘pure’ INa inhibition with rapid kinetics (with high concentrations of lidocaine and TTX) can effectively suppress ‘acute’ ACh-mediated AF in non-remodeled isolated preparations [79,80]. Although pilsicainide has been proclaimed to be a pure sodium channel blocker with slow kinetics and shown to be effective in AF suppression [81], this agent also inhibits HERG and occasionally produces long QT syndrome [82].

Ranolazine, propafenone and chronic amiodarone are effective in the suppression of ACh-mediated AF in canine isolated coronary-perfused right atria [5,45,48]. A major difference between ranolazine and propafenone is that at clinically relevant concentrations, which effectively suppress AF (10.0 and 1.5 μM, respectively), ventricular electrophysiological parameters are strongly affected by propafenone but not ranolazine. Ranolazine has been shown also to potently suppress isoproterenol-mediated AF associated with ischemia and reperfusion in canine isolated right atria [5]. Chronic amiodarone (40 mg/kg day for 6 weeks) prevents ACh-mediated AF, although causing moderate electrophysiological changes in canine isolated coronary-perfused left ventricular preparations [45]. The antiarrhythmic efficacy of lidocaine (at 21 μM, also a clinically relevant concentration) in this ACh-mediated AF model is relatively poor and its electrophysiologic effects in the ventricles are much greater than those of ranolazine [5]. Ranolazine or chronic amiodarone produce a greater prolongation of PRR than of APD, suggesting that their anti-AF effect is due largely to INa inhibition rather than IKr block. The ability of IKr block alone to suppress ACh-mediated AF is relatively poor.

The effectiveness of ranolazine to suppress AF in experimental models is consistent with the results of the MERLIN-TIMI 36 clinical study in which ranolazine treatment was associated with reduced incidence of supraventricular arrhythmias and a 30% reduction in new onset AF in patients with non-ST segment elevation acute coronary syndrome [83]. In a recent single-center study, ranolazine was effective in maintaining sinus rhythm in a cohort of AF patients (most of them with structural heart diseases) in whom more established AADs had failed [84]. AZD7009 has been shown to terminate AF and atrial flutter in 100% of cases in the canine sterile pericarditis model [51]. In a randomized, double-blind, placebo-controlled and multi-center study, the success rate of AZD7009 to terminate AF or atrial flutter in humans was impressive indeed (up to 70% in patients with mean AF duration of 43 days (2 – 90 days) and up to 52% in patients with AF lasting > 1 week) [85]. In another clinical study, conversion rate of AF of ≤ 30 day duration with AZD7009 was comparable to DC cardioversion (82 versus 83%, respectively) [86]. For comparison, the success rate of cardioversion of paroxysmal AF (< 7 day duration) with vernakalant is 51% [87]. Chronic amiodarone is arguably the best AAD for the maintenance of sinus rhythm [9]. Although the anti-AF mechanisms of chronic amiodarone is quite complex [87]. in canine ACh-model it is likely to be largely owing to the sodium channel blockade [45]. We are not aware of any clinical study in which the issue of atrial selective INa blockade has been specifically investigated.

Clinical data indicate that agents with relatively ‘slow’ (τ of ≥ 6 s) [63,73] sodium channel dissociation kinetics are capable of inducing ventricular fibrillation (VF) and ventricular tachycardia in structurally-compromised hearts (e.g., encainide, flecainide and propafenone) [7]. Atrial-selective INa blockers such as ranolazine and amiodarone have relatively rapid kinetics (unbinding τ = 0.2 – 1.6 s) [5,50], which may account for their safety in patients with structurally compromised hearts [8,83,88]. Despite their ability to inhibit IKr these agents either do not (ranolazine) or very rarely (amiodarone) induce TdP. The two drugs differ with respect to extra-cardiac toxicity. Chronic amiodarone is notorious for induction of extra-cardiac toxicity [8], whereas acute and chronic use of ranolazine has been shown to be safe [88].

The low proarrhythmic potential and antiarrhyhtmic efficacy of ranolazine and amiodarone in die ventricle is probably related to their ability to significantly block late INa [89,90]. A balanced inhibition of outward I& and inward late INa prevents the development of an exaggerated dispersion of repolarization as well as the induction of early afterdepolarizations, thus, averting both the substrate and trigger for development of TdP arrhythmias [89,91]. Late INa inhibition plays a key role in suppression of ventricular arrhythmias in a variety of pathological conditions such a long QT syndrome, acute ischemia and heart failure [89,91,92].

A combination of both atrial-selective INa block and atrial-specific IKur block is expected to exert a more potent effect than either approach alone for the management of AF. In remodeled atria, prolongation of APD90 secondary to IKur block can promote accumulation of INa block preferentially in atria by reducing diastolic interval in atria but not in ventricles, thus, contributing to the anti-AF effects of these agents. Interestingly, although the ability of IKr block alone to prolong APD and suppress AF is significantly reduced in remodeled goat atria, further inhibition of IKur/Ito restores their effectiveness, pointing to a synergism of this drug combination [93].

It is yet to be determined whether ranolazine preserves its atrial-selectivity for block of INa with chronic administration. Amiodarone seems to produce atrial-selective depression of INa-mediated parameters with both acute and chronic applications [45], Our preliminary data indicate that acute effects of amiodarone on sodium channel-mediated parameters studied in canine coronary-perfused atrial and ventricular preparations are atrial predominant as well (Burashnikov et al., unpublished).

The available data suggest that agents with combined inhibition of early INa (as the prime target, with relatively rapid kinetics), late INa, IKr and IKur could be atrial-selective and effective in suppressing AF without exerting a proarrhythmic effect in ventricular myocardium.

4.5 Atrial selectivity of lNa blockade in remodeled atria

AF is normally associated with electrical and structural remodeling, which can significantly modify pharmacologic response of atria to sodium and potassium channel blockers [40,94,95]. In this respect, it is important to recognize that the atrial selectivity of INa blockers has been demonstrated in ‘healthy atria and ventricles [5,45,47]. Fibrillating atria or atria susceptible to AF normally display short APDs and a depolarized RMP, which reduce and promote the effectiveness of INa block, respectively [63]. The ability of ranolazine, chronic amiodarone, lidocaine and propafenone to suppress sodium channel-depended parameters is reduced in ACh-treated canine atrial preparations, possessing an abbreviated APD [5,45,48], Alterations in INa density have been reported in remodeled canine [96], but not human [97], atria. Conduction velocity is not changed in remodeled atria of the goat [4]. V05 of INa inactivation is shifted by +10 mV in cells isolated from AF versus sinus rhythm patients [97], which may reduce atrial sensitivity to INa blockers. This may account for the lower antiarrhythmic efficacy of INa block in persistent AF [1]. This limitation may apply to atrial selective INa blockers as well. Note, however, that the potency of Class IC agents was not altered by atrial tachypacing-induced remodeling in goats [98].

4.6 Influence of atrial-selective agents on ventricular arrhythmias

Ventricular arrhythmias commonly occur under pathophysiological conditions associated with ischemia, infarction, long QT syndrome and hypertrophy. These conditions can importantly modulate the response to sodium and potassium channel blockers. This may explain why atrial-selective agents (as determined by their actions in ‘healthy hearts) can exert significant electrophysiological and antiarrhythmic actions in ‘abnormal’ ventricles. For example, the atrial selective IKur/Im blocker AVE0118 effectively suppresses ventricular arrhythmias occurring in the setting of ischemia in dogs [99]. Ranolazine reduces the incidence of ventricular arrhydimias associated with acute coronary and long QT syndrome, an effect attributed to the action of ranolazine to block late INa [83,89,100]. Although generally used to treat atrial arrhythmias, amiodarone can effectively suppress ventricular arrhythmias [9].

4.7 Molecular biology and genetics of cardiac sodium channels

The genetic basis for the atrioventricular difference in biophysical properties of the sodium channel is not fully understood. Whereas the α-subunit of the cardiac sodium channel (SCN5A) is the same in atrial and ventricular cells, chamber-specific differences in the stoichiometry of auxiliary subunits may exist and contribute to the distinct characteristics of atrial and ventricular sodium channels. Four β sub-units of the sodium channel have been identified in the heart (SCN1B, SCN2B, SCN3B and SCN4B) [101]. SCN3B is present in the ventricles but not in the atria of sheep and rat hearts [102,103]. SCN1B was found both in atria and ventricles of guinea pigs, rat and humans [102104]. SCN1B is more strongly expressed in atria versus ventricles in human [104]. The expression level of SCN1B is much greater than that of SCN3B in the human heart [104]. Interestingly, co-expression of SCN3B and SCN5A in Xenopus oocytes shifts the h-curve to the right, compared to SCN5A alone or SCN5A + SCN1B [102]. This may contribute to the atrioventricular difference in the steady-state inactivation curves (Figure 5). In contrast, co-expression of SCN5A and SCN3B in TSA201 cells shifts the h-curve to the left, compared to SCN5A alone or SCN5A + SCN1B co-expression [105]. A left-ward shift of h-curve was also observed when SCN5A is co-expressed with SCN3B in Chinese hamster ovary cells [103]. SCN1B null mice have an increased Navl.5 protein expression and acutely isolated ventricular myocytes from these mice have an increased early INa and late INa [106]. It is recognized that β-subunits of various channels can interact with each other, affecting phenotypical manifestations of the channels. INa may be influenced by KChIP2 (a β subunit of KV4.3 channels, producing Ito) and SCN1B, in turn, may affect Ito parameters [107].

There are mutations or rare variants in SCN5A that have been associated with familial AF [108,109]. These mutations or polymorphisms in SCN5A, however, are likely to account for only a minority of AF cases [108-110]. It is not known whether atrial-selective INa blockers will be of benefit or harm in AF patients with SCN5A mutations. However, because INa blockers apparently have never been shown to induce AF de novo (in contrast to VF) it is not clear whether INa block will promote AF. It is, however, noteworthy that SCN5A mutations have been associated with AF in patients with Brugada [111].

5. Competitive environment

The concept of the atrial selective block of INa for the management of AF is relatively new [5]. Few atrial selective INa blockers have been identified until now. AADs thus far shown to depress sodium channel-dependent parameters in a atrial-selective manner in experimental models and to effectively suppress AF (in experimental models and in the clinic) are ranolazine, chronic amiodarone and AZD7009 (Table 1) [5,45,51,8386,112]. Vernakalant, by virtue of its effect to block INa with rapid kinetics and to inhibit I^ [33] may also prove to be an atrial-selective INa blocker. Vernakalant, a cardiome product, has received an approval letter for acute termination of AF and is undergoing Phase III clinical trials for maintenance therapy. We are not aware of any programs specifically designed for the development of atrial-selective INa blockers.

Table 1
Agents that suppress INa-dependent parameters in an atrial-selective manner and are effective against atrial fibrillation.

6. Potential development issues

The chemical structure contributing to atrial-selective depression of INa-dependent parameters is not known. Accordingly, there is no clear directive for development of safe and effective drugs with this unique feature. Because long-term use of ranolazine has been found to be free of significant intra- or extra-cardiac adverse effects [88], the chemical structure of ranolazine may be a ‘safe model’ to use for the development of atrial-selective INa blockers.

7. Conclusion

A growing body of experimental and clinical evidence suggests that atrial-selective sodium channel blockers may offer a safe and effective strategy for the management of AF. These agents, which include ranolazine, amiodarone and AZD7009, are effective in suppressing AF and/or preventing its re-induction, without the risk of with a low risk of induction of ventricular tachycardia/VF or TdP [8386,112]. The degree to which the clinical effectiveness of these multi-ion channel blockers depends on their potency to depress sodium channel-dependent parameters remains to be explained. Available data suggest that the two principal factors contributing to atrial selectivity are: i) rapid dissociation of the drug from the sodium channels; and ii) atrial selective APD prolongation secondary to inhibition of IKr and IKur The clinical observations coupled with recent experimental data suggest that further studies specifically designed to evaluate the potential role of atrial-selective sodium channel blockers as antiarrhythmics are warranted.

8. Expert opinion

The cellular and molecular mechanisms responsible for the initiation and maintenance of AF are multi-factorial and pathology- and stage-specific. Dynamic factors such as autonomic influences are well-known to promote AF [113]. The development of AADs has been empiric, in part due to: i) our relatively poor understanding of cellular and molecular mechanisms of AF under different pathophysiological conditions; and ii) incomplete knowledge of the optimal chemical structure needed to appropriately interact with anti-AF targets without inducing ventricular arrhythmias or extra-cardiac adverse effects.

Two principal factors have recently been suggested as contributing to atrial selectivity of sodium channel blockers: i) relatively rapid dissociation of the drug from the sodium channels; and ii) atrial predominant APD prolongation secondary to inhibition of IKr and IKur. The most effective anti-AF agent amiodarone, initially introduced as an anti-anginal agent in 1962 [8], was recently shown to possess both of these actions and to be an atrial-selective sodium channel blocker [45]. Ranolazine, an antianginal agent with a ion channel profile similar to that of amiodarone, also possesses these two key factors and has been shown to be highly selective in its ability to suppress INa-mediated parameters [5]. Vernakalant and AZD7009, recently developed as IKur blockers, also potently inhibit INa and IKr and their anti-AF efficacy may be largely owing to INa block rather than IKur block [22,37]. Anti-AF efficacy of another purported selective IKur blocker AVE0118 may be largely due with its inhibition of CA-IK-ACh, as well as inhibition of INa, rather than IKur. [36,52]. Although a great deal of effort has been devoted to development of specific IKur. blockers [32], it remains to be determined if inhibition of IKur alone is sufficient to effectively suppress AF (see sections 2.0 and 4.1 for discussion) [27,41,47].

Interestingly, many of the prominent new investigational anti-AF agents block INa (such as dronedarone, vernakalant, AZD7009, ranolazine, AVE0118). All three agents investigated for depressing sodium channel-dependent parameters (i.e., ranolazine, AZD7009 and AVE0118) do so in an atrial-predominant manner [5,51,52]. Atrioventricular differences of the effects of vernakalant and dronedarone on sodium channel-dependent parameters have not been studied. However, vernakalant, like ranolazine and amiodarone, blocks INa with rapid kinetics and inhibits IKr [33]. Dronedarone is an analogue of amiodarone possessing ion channel block profile similar to its predecessor. Although the anti-AF effectiveness of amiodarone has been ascribed to a large number of factors, including APD/ERP prolongation, reduction of dispersion of repolarization, induction of PRR, prolongation of excitable gap, suppression of triggered activity, inhibition of atrial electrical and structural remodeling and blocking of (β-adreno-receptors [114], recent studies suggest that the drug’s actions to depress sodium channel-dependent parameters may predominate [45].

The prolongation of APD/ERP or depression of INa-mediated parameters by AADs may not necessarily correlate with the ability of these agents to suppress clinical persistent AF, due in part to the fact that long-standing AF is associated with important structural remodeling. The ability INa blockers to suppress persistent AF is significantly reduced [1], despite the fact that the ability of the drugs to depress Independent parameters is not altered, at least in tachypacing-induced remodeled goat atria [94,98]. IKr block (dofetilide) is most effective in terminating clinically persistent AF [1], despite the fact that its ability to prolong APD/ERP is significantly reduced in remodeled atria [94].

There are major differences in the proarrhythmic liability of early INa or IKr blockers in the atria versus ventricles of the heart. INa blockers are capable of readily inducing severe proarrhythmia in the ventricles (e.g., VF), but apparently never (AF) or rarely (e.g., atrial flutter [115]) in atria. The proarrhythmic action of INa block in the ventricles is believed to be owing to conduction slowing. Why then can INa blockers slow conduction in atria without inducing AF? IKr blockers also readily induce TdP arrhythmias in ventricles, but not in atria [72,116]. The mechanisms underlying protection of atria from AAD-induced proarrhythmias are poorly understood and await further investigation. This information may be of benefit in the design of safer and more effective drugs for the management of AF.

The stigma of the CAST [7] resulted in pharmaceutical companies shying away from the development of INa blockers as antiarrhythmics for nearly 20 years. Inhibition of INa has proven to be effective in a variety of AF pathologies [1,117]; however, its proarrhythmic actions in structurally compromised ventricles have limited the utility of this approach. The advent of atrial-selective INa blockers may offer an important new strategy for the safe and effective management of AF under a wide variety of pathophysiological states associated with AF.

Acknowledgments

Declaration of interest

Charles Antzelevitch has received research grants and consultantship from CV Therapeutics and AstraZeneca. He has also received research grants from Epix, Solvay, Neurosearch, Lundbeck, Cardiome and Devgen. The paper was supported by grant HL47678 from NHLBI (CA) and the Masons of New York State and Florida.

Bibliography

Papers of special note have been highlighted as either of interest (•) or of considerable interest (••) to readers.

1. Fuster V, Ryden LE, Cannom DS, et al. ACC/AHA/ESC 2006 guidelines for the management of patients with atrial fibrillation–executive summary: a report of the American college of cardiology/American heart association task force on practice guidelines and the European society of cardiology committee for practice guidelines (Writing Committee to Revise the 2001 Guidelines for the Management of Patients With Atrial Fibrillation) J Am Coll Cardiol. 2006;48:854–906. [PubMed]
2. Reiffel JA. Rate versus rhythm control pharmacotherapy for atrial fibrillation: where are we in 2008? J Atrial Fibrillation. 2008;1:31–47.
3. Calkins H, Brugada J, Packer DL, et al. HRS/EHRA/ECAS expert Consensus Statement on catheter and surgical ablation of atrial fibrillation: recommendations for personnel, policy, procedures and follow-up. A report of the Heart Rhythm Society (HRS) Task Force on catheter and surgical ablation of atrial fibrillation. Heart Rhythm. 2007;4:816–61. [PubMed]
4••. Wijffels MC, Kirchhof CJ, Dorland R, et al. Aerial fibrillation begets atrial fibrillation. A study in awake chronically instrumented goats. Circulation. 1995;92:1954–68. Landmark study demonstrating that rapid activation of the atria during AF leads to electrical remodeling, which make subsequent episodes of AF easier to induce and more enduring. [PubMed]
5••. Burashnikov A, Di Diego JM, Zygmunt AC, et al. Atrium-selective sodium channel block as a strategy for suppression of atrial fibrillation: differences in sodium channel inactivation between atria and ventricles and the role of ranolazine. Circulation. 2007;116:1449–57. This paper first introduced the concept of atrial-selective sodium channel block as a treatment for AF. Major atrioventricular differences in biophysical properties of sodium channels were uncovered. [PMC free article] [PubMed]
6. Naccarelli GV, Gonzalez MD. Atrial fibrillation and the expanding role of catheter ablation: do antiarrhythmic drugs have a future? J Cardiovasc Pharmacol. 2008;52:203–9. [PubMed]
7•. CAST Investigators. Preliminary report: effect of encainide and flecainide on mortality in a randomized trial of arrhythmia suppression after myocardial infarction. N Engl J Med. 1989;321:406–12. This pivotal study revealed that potent INa blockers (Class 1C) increase mortality in patients with structural heart diseases. There results had a dramatic impact on development of antiarrhythmic agents over the past 20 years. [PubMed]
8. Singh BN. Amiodarone as paradigm for developing new drugs for atrial fibrillation. J Cardiovasc Pharmacol. 2008;52:300–5. [PubMed]
9. Goldschlager N, Epstein AE, Naccarelli GV, et al. A practical guide for clinicians who treat patients with amiodarone: 2007. Heart Rhythm. 2007;4:1250–9. [PubMed]
10. Wazni OM, Marrouche NF, Martin DO, et al. Radiofrequency ablation vs antiarrhythmic drugs as first-line treatment of symptomatic atrial fibrillation: a randomized trial. JAMA. 2005;293:2634–40. [PubMed]
11. Pappone C, Radinovic A, Manguso F, et al. Atrial fibrillation progression and management: a 5-year prospective follow-up study. Heart Rhythm. 2008;5:1501–7. [PubMed]
12. Jais P, Cauchemez B, Macle L, et al. Catheter ablation versus antiarrhythmic drugs for atrial fibrillation: the A4 study. Circulation. 2008;118:2498–505. [PubMed]
13. Callans DJ. Apples and oranges: comparing antiarrhythmic drugs and catheter ablation for treatment of atrial fibrillation. Circulation. 2008;118:2488–90. [PubMed]
14. Wyse DG, Waldo AL, DiMarco JP, et al. A comparison of rate control and rhythm control in patients with atrial fibrillation. N Engl J Med. 2002;347:1825–33. [PubMed]
15. Roy D, Talajic M, Nattel S, et al. Rhythm control versus rate control for atrial fibrillation and heart failure. N Engl J Med. 2008;358:2667–77. [PubMed]
16. Cain ME, Curtis AB. Rhythm control in atrial fibrillation–one setback after another. N Engl J Med. 2008;358:2725–7. [PubMed]
17. Corley SD, Epstein AE, DiMarco JP, et al. Relationships between sinus rhythm, treatment, and survival in the Atrial Fibrillation Follow-Up Investigation of Rhythm Management (AFFIRM) Study. Circulation. 2004;109:1509–13. [PubMed]
18. Steinberg JS, Sadaniantz A, Kron J, et al. Analysis of cause-specific mortality in the Atrial Fibrillation Follow-up Investigation of Rhythm Management (AFFIRM) study. Circulation. 2004;109:1973–80. [PubMed]
19. Curtis AB, Gersh BJ, Corley SD, et al. Clinical factors that influence response to treatment strategies in atrial fibrillation: the Atrial Fibrillation Follow-up Investigation of Rhythm Management (AFFIRM) study. Am Heart J. 2005;149:645–9. [PubMed]
20. Savelieva I, Camm J. Anti-arrhythmic drug therapy for atrial fibrillation: current anti-arrhythmic drugs, investigational agents, and innovative approaches. Europace. 2008;10:647–65. [PubMed]
21. Nattel S, Carlsson L. Innovative approaches to anti-arrhythmic drug therapy. Nat Rev Drug Discov. 2006;5:1034–49. [PubMed]
22. Burashnikov A, Antzelevitch C. How do atrial-selective drugs differ from anriarrhythmic drugs currently used in the treatment of atrial fibrillation? J Atrial Fibrillation. 2008;1:98–107. [PMC free article] [PubMed]
23. Nattel S, Burstein B, Dobrev D. Atrial remodeling and atrial fibrillation: mechanisms and implications. Circ Arrhythm Electrophysiol. 2008;1:62–73. [PubMed]
24. Goette A, Bukowska A, Lendeckel U. Non-ion channel blockers as anti-arrhythmic drugs (reversal of structural remodeling) Curr Opin Pharmacol. 2007;7:219–24. [PubMed]
25. Savelieva I, Camm J. Statins and polyunsaturated fatty acids for treatment of atrial fibrillation. Nat Clin Pract Cardiovasc Med. 2008;5:30–41. [PubMed]
26. Dobrev D, Friedrich A, Voigt N, et al. The G protein-gated potassium current IK, ACh is constitutively active in patients with chronic atrial fibrillation. Circulation. 2005;112:3697–706. [PubMed]
27. Ehrlich JR, Nattel S. Atrial-selective pharmacological therapy for atrial fibrillation: hype or hope? Curr Opin Cardiol. 2009;24:50–5. [PubMed]
28. Cha TJ, Ehrlich JR, Chartier D, et al. Kir3-based inward rectifier potassium current: potential role in atrial tachycardia remodeling effects on atrial repolarization and arrhythmias. Circulation. 2006;113:1730–7. [PubMed]
29. Hashimoto N, Yamashita T, Tsuruzoe N. Tertiapin, a selective IK, ACh blocker, terminates atrial fibrillation with selective atrial effective refractory period prolongation. Pharmacol Res. 2006;54:136–41. [PubMed]
30. Guerra JM, Everett TH, Lee KW, et al. Effects of the gap junction modifier rotigaptide (ZP123) on atrial conduction and vulnerability to atrial fibrillation. Circulation. 2006;114:110–8. [PubMed]
31. Shiroshita-Takeshita A, Sakabe M, Haugan K, et al. Model-dependent effects of the gap junction conduction-enhancing antiarrhythmic peptide rotigaptide (ZP123) on experimental atrial fibrillation in dogs. Circulation. 2007;115:310–8. [PubMed]
32. Ford JW, Milnes JT. New drugs targeting the cardiac ultra-rapid delayed-rectifier current (I Kur): rationale, pharmacology and evidence for potential therapeutic value. J Cardiovasc Pharmacol. 2008;52:105–20. [PubMed]
33. Fedida D. Vernakalant (RSD1235): a novel, atrial-selective antifibrillatory agent. Expert Opin Investig Drugs. 2007;16:519–32. [PubMed]
34. Carlsson L, Chartier D, Nattel S. Characterization of the in vivo and in vitro electrophysiological effects of the novel antiarrhythmic agent AZD7009 in atrial and ventricular tissue of the dog. J Cardiovasc Pharmacol. 2006;47:123–32. [PubMed]
35. Blaauw Y, Gogelein H, Tieleman RG, et al. “Early” class III drugs for the treatment of atrial fibrillation: efficacy and atrial selectivity of AVE0118 in remodeled atria of the goat. Circulation. 2004;110:1717–24. [PubMed]
36. Christ T, Wettwer E, Voigt N, et al. Pathology-specific effects of the IKur/Ito/IK, ACh blocker AVE0118 on ion channels in human chronic atrial fibrillation. Br J Pharmacol. 2008;154:1619–30. [PMC free article] [PubMed]
37. Burashnikov A, Antzelevitch C. Can inhibition of IKur promote atrial fibrillation? Heart Rhythm. 2008;5:1304–9. [PMC free article] [PubMed]
38. Feng J, Xu D, Wang Z, et al. Ultrarapid delayed rectifier current inactivation in human atrial myocytes: properties and consequences. Am J Physiol. 1998;275:H1717–25. [PubMed]
39. Van Wagoner DR, Pond AL, McCarthy PM, et al. Outward K + current densities and Kv1.5 expression are reduced in chronic human atrial fibrillation. Circ Res. 1997;80:772–81. [PubMed]
40. Wettwer E, Hala O, Christ T, et al. Role of IKur in controlling action potential shape and contractility in the human atrium: influence of chronic atrial fibrillation. Circulation. 2004;110:2299–306. [PubMed]
41. Burashnikov A, Antzelevitch C. New pharmacological strategies for the treatment of atrial fibrillation. Ann Noninvasive Electrocardiol. 2009 In press. [PMC free article] [PubMed]
42. Olson TM, Alekseev AE, Liu XK, et al. Kv1.5 channelopathy due to KCNA5 loss-of-function mutation causes human atrial fibrillation. Hum Mol Genet. 2006;15:2185–91. [PubMed]
43. Burashnikov A, Mannava S, Antzelevitch C. Transmembrane action potential heterogeneity in the canine isolated arterially-perfused atrium: effect of IKr and Ito/IKur block. Am J Physiol. 2004;286:H2393–400. [PubMed]
44. Nattel S. New ideas about atrial fibrillation 50 years on. Nature. 2002;415:219–26. [PubMed]
45••. Burashnikov A, Di Diego JM, Sicouri S, et al. Atrial-selective effects of chronic amiodarone in the management of atrial fibrillation. Heart Rhythm. 2008;5:1735–42. This study revealed that chronic amiodarone exerts its anti-AF actions through an atrial-selective depression of sodium channel-dependent parameters. [PMC free article] [PubMed]
46. Burashnikov A, Di Diego JM, Zygmunt AC, et al. A trial-selective sodium channel block as a strategy for suppression of atrial fibrillation. Ann NY Acad Sci. 2008;1123:105–12. [PMC free article] [PubMed]
47•. Burashnikov A, Antzelevitch C. Atrial-selective sodium channel blockers: do they exist? J Cardiovasc Pharmacol. 2008;52:121–8. A comprehensive review on atrial selective sodium channel blockers. [PMC free article] [PubMed]
48. Burashnikov A, Belardinelli L, Antzelevitch C. Ranolazine and propafenone both suppress atrial fibrillation but ranolazine unlike propafenone does it without prominent effects on ventricular myocardium. Heart Rhythm. 2007;4:S163.
49. Antzelevitch C, Belardinelli L, Zygmunt AC, et al. Electrophysiologic effects of ranolazine: a novel anti-anginal agent with antiarrhythmic properties. Circulation. 2004;110:904–10. [PMC free article] [PubMed]
50. Kodama I, Kamiya K, Toyama J. Amiodarone: ionic and cellular mechanisms of action of the most promising class III agent. Am J Cardiol. 1999;84:20R–8R. [PubMed]
51•. Goldstein RN, Khrestian C, Carlsson L, et al. Azd7009: a new antiarrhythmic drug with predominant effects on the atria effectively terminates and prevents reinduction of atrial fibrillation and flutter in the sterile pericarditis model. J Cardiovasc Electrophysiol. 2004;15:1444–50. This study demonstrated that AZD7009, an effective anti-AF agent, produces atrial selective depression of sodium channel-dependent parameters (diastolic threshold of excitation and conduction velocity). [PubMed]
52. Burashnikov A, Barajas-Martinez H, Hu D, et al. The atrial-selective potassium channel blocker AVE0118 prolongs effective refractory period in canine atria by inhibiting sodium channels [abstract] Heart Rhythm. 2009;6:S98.
53. Regan CP, Kiss L, Stump GL, et al. Atrial antifibrillatory effects of structurally distinct IKur blockers 3-[(dimethylamino) methyl]-6-methoxy-2-methyl-4-phenylisoquinolin-l(2H)-one and 2-phenyl-l,l-dipyridin-3-yl-2-pyrrolidin-1-yl-ethanol in dogs with underlying heart failure. J Pharmacol Exp Ther. 2008;324:322–30. [PubMed]
54. Courtemanche M, Ramirez RJ, Nattel S. Ionic targets for drug therapy and atrial fibrillation-induced electrical remodeling: insights from a mathematical model. Cardiovasc Res. 1999;42:477–89. [PubMed]
55. Wirth KJ, Brendel J, Steinmeyer K, et al. In vitro and in vivo effects of the atrial selective antiarrhythmic compound AVE1231. J Cardiovasc Pharmacol. 2007;49:197–206. [PubMed]
56. Li GR, Wang HB, Qin GW, et al. Acacetin, a natural flavone, selectively inhibits human atrial repolarization potassium currents and prevents atrial fibrillation in dogs. Circulation. 2008;117:2449–57. [PubMed]
57. Singh BN, Vaughan-Williams EM. The effect of amiodarone, a new anti-anginal drug, on cardiac muscle. Br J Pharmacol. 1970;39:657–67. [PMC free article] [PubMed]
58. Kodama I, Toyama J, Takanaka C, et al. Block of activated and inactivated sodium channels by class I antiarrhythmic drugs studied by using the maximum upstroke velocity (Vmax) of action potential in guinea-pig cardiac muscles. J Mol Cell Cardiol. 1987;19:367–77. [PubMed]
59. Langenfeld H, Weirich J, Kohler C, et al. Comparative analysis of the action of class I antiarrhythmic drugs (lidocaine, quinidine, and prajmaline) in rabbit atrial and ventricular myocardium. J Cardiovasc Pharmacol. 1990;15:338–45. [PubMed]
60. Nemeth M, Virag L, Hala O, et al. The cellular electrophysiological effects of tedisamil in human atrial and ventricular fibers. Cardiovasc Res. 1996;31:246–8. [PubMed]
61. Persson F, Andersson B, Duker G, et al. Functional effects of the late sodium current inhibition by AZD7009 and lidocaine in rabbit isolated atrial and ventricular tissue and Purkinje fibre. Eur J Pharmacol. 2007;558:133–43. [PubMed]
62. Hondeghem LM, Katzung BG. Mechanism of action of antiarrhythmic drugs. In: Sperelakis N, editor. Physiology and Pathophysiology of the Heart. 3. Kluwer Academic Publishers; 1995. pp. 589–603.
63. Whalley DW, Wendt DJ, Grant AO. Basic concepts in cellular cardiac electrophysiology: part II: block of ion channels by antiarrhythmic drugs. PACE. 1995;18:1686–704. [PubMed]
64. Spinelli W, Parsons RW, Colatsky TJ. Effects ofWAY-123,398, a new Class-III antiarrhythmic agent, on cardiac refractoriness and ventricular fibrillation threshold in anesthetized dogs – a comparison with UK-68798, e-4031, and DL- Sotalol. J Cardiovasc Pharmacol. 1992;20:913–22. [PubMed]
65. Wiesfeld AC, De Langen CD, Crijns HJ, et al. Rate-dependent effects of the class III antiarrhythmic drug almokalant on refractoriness in the pig. J Cardiovasc Pharmacol. 1996;27:594–600. [PubMed]
66. Baskin EP, Lynch JJ., Jr Differential atrial versus ventricular activities of class III potassium channel blockers. J Pharmacol Exp Ther. 1998;285:135–42. [PubMed]
67. Stump GL, Wallace AA, Regan CP, et al. In vivo antiarrhythmic and cardiac electrophysiologic effects of a novel diphenylphosphine oxide IKur blocker (2-isopropyl-5-methylcyclohexyl) diphenylphosphine oxide. J Pharmacol Exp Ther. 2005;315:1362–7. [PubMed]
68. Wang J, Feng J, Nattel S. Class III antiarrhythmic drug action in experimental atrial fibrillation. Differences in reverse use dependence and effectiveness between d-sotalol and the new antiarrhythmic drug ambasilide. Circulation. 1994;90:2032–40. [PubMed]
69. Echt DS, Berte LE, Clusin WT, et al. Prolongation of the human monophasic action potential by sotalol. Am J Cardiol. 1982;50:1082–6. [PubMed]
70. Buchanan LV, LeMay RJ, Walters RR, et al. Antiarrhythmic and electrophysiologic effects of intravenous ibutilide and sotalol in the canine sterile pericarditis model. J Cardiovasc Electrophysiol. 1996;7:113–9. [PubMed]
71. Antzelevitch C, Shimizu W, Yan GX, et al. The M cell: its contribution to the ECG and to normal and abnormal electrical function of the heart. J Cardiovasc Electrophysiol. 1999;10:1124–52. [PubMed]
72. Burashnikov A, Antzelevitch C. Late-phase 3 EAD. A unique mechanism contributing to initiation of atrial fibrillation. PACE. 2006;29:290–5. [PMC free article] [PubMed]
73. Carmeliet E, Mubagwa K. Antiarrhythmic drugs and cardiac ion channels: mechanisms of action. Prog Biophys Mol Biol. 1998;70:l–72. [PubMed]
74. Wang GK, Calderon J, Wang SY. State- and use-dependent block of muscle Nav1.4 and neuronal Nav1.7 noltage-gated Na + channel isoforms by ranolazine. Mol Pharmacol. 2008;73:940–8. [PMC free article] [PubMed]
75. Kodama I, Kamiya K, Toyama J. Cellular electropharmacology of amiodarone. Cardiovasc Res. 1997;35:13–29. [PubMed]
76. Golod DA, Kumar R, Joyner RW. Determinants of action potential initiation in isolated rabbit atrial and ventricular myocytes. Am J Physiol. 1998;274:H1902–13. [PubMed]
77. Hiroe K, Hisatome I, Tanaka Y, et al. Tonic block of the Na + current in single atrial and ventricular guinea-pig myocytes, by a new antiarrhythmic drug, Ro 22–9194. Fundam Clin Pharmacol. 1997;11:402–7. [PubMed]
78. Li GR, Lau CP, Shrier A. Heterogeneity of sodium current in atrial vs epicardial ventricular myocytes of adult guinea pig hearts. J Mol Cell Cardiol. 2002;34:1185–94. [PubMed]
79. Kneller J, Kalifa J, Zou R, et al. Mechanisms of atrial fibrillation termination by pure sodium channel blockade in an ionically-realistic mathematical model. Circ Res. 2005;96:e35–e47. [PubMed]
80. Comtois P, Sakabe M, Vigmond EJ, et al. Mechanisms of atrial fibrillation termination by rapidly unbinding Na + channel blockers. Insights from mathematical models and experimental correlates. Am J Physiol Heart Circ Physiol. 2008;295:H1489–H1504. [PubMed]
81. Kumagai K, Nakashima H, Tojo H, et al. Pilsicainide for atrial fibrillation. Drugs. 2006;66:2067–73. [PubMed]
82. Wu LM, Orikabe M, Hirano Y, et al. Effects of Na + channel blocker, pilsicainide, on HERG current expressed in HEK-293 cells. J Cardiovasc Pharmacol. 2003;42:410–8. [PubMed]
83•. Scirica BM, Morrow DA, Hod H, et al. Effect of ranolazine, an antianginal agent with novel electrophysiological properties, on the incidence of arrhythmias in patients with non ST-segment elevation acute coronary syndrome: results from the Metabolic Efficiency With Ranolazine for Less Ischemia in Non ST-Elevation Acute Coronary Syndrome Thrombolysis in Myocardial Infarction 36 (MERLIN-TIMI 36) randomized controlled trial. Circulation. 2007;116:1647–52. This clinical trial demonstrated that ranolazine is safe and effective in suppressing supraventricular as well as ventricular arrhythmias in patients with non-ST-segment-elevation acute coronary syndrome. [PubMed]
84. Murdock DK, Overton N, Kersten M, et al. The effect of ranolazine on maintaining sinus rhythm in patients with resistant atrial fibrillation. Indian Pacing Electrophysiol J. 2008;8:175–81. [PMC free article] [PubMed]
85. Crijns HJ, Van GI, Walfridsson H, et al. Safe and effective conversion of persistent atrial fibrillation to sinus rhythm by intravenous AZD7009. Heart Rhythm. 2006;3:1321–31. [PubMed]
86. Geller JC, Egstrup K, Kulakowski P, et al. Rapid conversion of persistent atrial fibrillation to sinus rhythm by intravenous AZD7009. J Clin Pharmacol. 2009;49:312–22. [PubMed]
87. Roy D, Pratt CM, Torp-Pedersen C, et al. Vernakalant hydrochloride for rapid conversion of atrial fibrillation. A phase 3, randomized, placebo-controlled trial. Circulation. 2008;117:1518–25. [PubMed]
88. Chaitman BR. Ranolazine for the treatment of chronic angina and potential use in other cardiovascular conditions. Circulation. 2006;113:2462–72. [PubMed]
89. Antzelevitch C, Belardinelli L, Wu L, et al. Electrophysiologic properties and antiarrhythmic actions of a novel anti-anginal agent. J Cardiovasc Pharmacol Ther. 2004;9(Suppl 1):S65–S83. [PubMed]
90. Maltsev VA, Sabbah HN, Undrovinas AI. Late sodium current is a novel target for amiodarone: studies in failing human myocardium. J Mol Cell Cardiol. 2001;33:923–32. [PubMed]
91. Antzelevitch C. Electrical heterogeneity, cardiac arrhythmias, and the sodium channel. Circ Res. 2000;87:964–5. [PubMed]
92. Shryock JC, Belardinelli L. Inhibition of late sodium current to reduce electrical and mechanical dysfunction of ischaemic myocardium. Br J Pharmacol. 2008;153:1128–32. [PMC free article] [PubMed]
93. Blaauw Y, Schotten U, van HA, et al. Cardioversion of persistent atrial fibrillation by a combination of atrial specific and non-specific class III drugs in the goat. Cardiovasc Res. 2007;75:89–98. [PubMed]
94. Duytschaever M, Blaauw Y, Allessie M. Consequences of atrial electrical remodeling for the anti-arrhythmic action of class IC and class III drugs. Cardiovasc Res. 2005;67:69–76. [PubMed]
95. Linz DK, Afkham F, Itter G, et al. Effect of atrial electrical remodeling on the efficacy of antiarrhythmic drugs: comparison of amiodarone with IKr- and Ito/IKur-blockade in vivo strial electrical remodeling and antiarrhythmic drugs. J Cardiovasc Electrophysiol. 2007;18:1313–20. [PubMed]
96. Gaspo R, Bosch RF, Bou-Abboud E, et al. Tachycardia-induced changes in Na + current in a chronic dog model of atrial fibrillation. Circ Res. 1997;81:1045–52. [PubMed]
97. Bosch RF, Zeng X, Grammer JB, et al. Ionic mechanisms of electrical remodeling in human atrial fibrillation. Cardiovasc Res. 1999;44:121–31. [PubMed]
98. Eijsbouts S, Ausma J, Blaauw Y, et al. Serial cardioversion by class IC drugs during 4 months of persistent atrial fibrillation in the goat. J Cardiovasc Electrophysiol. 2006;17:648–54. [PubMed]
99. Billman GE, Kukielka M. Novel transient outward and ultra-rapid delayed rectifier current antagonist, AVE0118, protects against ventricular fibrillation induced by myocardial ischemia. J Cardiovasc Pharmacol. 2008;51:352–8. [PubMed]
100. Antzelevitch C. Ranolazine: a new antiarrhythmic agent for patients with non-ST-segment elevation acute coronary syndromes? Nat Clin Pract Cardiovasc Med. 2008;5:248–9. [PMC free article] [PubMed]
101. Brackenbury WJ, Isom LL. Voltage-gated Na + channels: potential for b subunits as therapeutic targets. Expert Opin Ther Targets. 2008;12:1191–203. [PMC free article] [PubMed]
102. Fahmi AI, Patel M, Stevens EB, et al. The sodium channel b-subunit SCN3b modulates the kinetics of SCN5a and is expressed heterogeneously in sheep heart. J Physiol. 2001;537:693–700. [PubMed]
103. Ko SH, Lenkowski PW, Lee HC, et al. Modulation of Nav1.5 by b1- and b3-subunit co-expression in mammalian cells. Pflugers Arch. 2005;449:403–12. [PubMed]
104. Gaborit N, Le BS, Szuts V, et al. Regional and tissue specific transcript signatures of ion channel genes in the non-diseased human heart. J Physiol. 2007;582:675–93. [PubMed]
105. Hu D, Zygmunt AC, Burashnikov A, et al. Sodium channel of canine atrial and ventricular cells differ with respect to voltage dependence of inactivation [abstract] Heart Rhythm. 2007;4:S148.
106. Lopez-Santiago LF, Meadows LS, Ernst SJ, et al. Sodium channel Scn1b null mice exhibit prolonged QT and RR intervals. J Mol Cell Cardiol. 2007;43:636–47. [PMC free article] [PubMed]
107. Deschenes I, Armoundas AA, Jones SP, et al. Post-transcriptional gene silencing of KChIP2 and Navb1 in neonatal rat cardiac myocytes reveals a functional association between Na and Ito currents. J Mol Cell Cardiol. 2008;45:336–46. [PMC free article] [PubMed]
108. Ellinor PT, Nam EG, Shea MA, et al. Cardiac sodium channel mutation in atrial fibrillation. Heart Rhythm. 2008;5:99–105. [PubMed]
109. Darbar D, Kannankeril PJ, Donahue BS, et al. Cardiac sodium channel (SCN5A) variants associated with atrial fibrillation. Circulation. 2008;117:1927–35. [PMC free article] [PubMed]
110. Chen LY, Ballew JD, Herron KJ, et al. A common polymorphism in SCN5A is associated with lone atrial fibrillation. Clin Pharmacol Ther. 2007;81:35–41. [PMC free article] [PubMed]
111. Kusano KF, Taniyama M, Nakamura K, et al. Atrial fibrillation in patients with Brugada syndrome relationships of gene mutation, electrophysiology, and clinical backgrounds. J Am Coll Cardiol. 2008;51:1169–75. [PubMed]
112. Zimetbaum P. Amiodarone for atrial fibrillation. N Engl J Med. 2007;356:935–41. [PubMed]
113. Chen PS. Neural mechanisms of atrial fibrillation. Heart Rhythm. 2006;3:1373–7. [PubMed]
114. Singh BN. Amiodarone: a multifaceted antiarrhythmic drug. Curr Cardiol Rep. 2006;8:349–55. [PubMed]
115. Nabar A, Rodriguez LM, Timmermans C, et al. Class IC antiarrhythmic drug induced atrial flutter: electrocardiographic and electrophysiological findings and their importance for long term outcome after right atrial isthmus ablation. Heart. 2001;85:424–9. [PMC free article] [PubMed]
116. Vincent GM. Atrial arrhythmias in the inherited long QT syndrome: laboratory quirk or clinical arrhydimia? J Cardiovasc Electrophysiol. 2003;14:1034–5. [PubMed]
117. Alboni P, Botto GL, Baldi N, et al. Outpatient treatment of recent-onset atrial fibrillation with the “pill-in-the-pocket” approach. N Engl J Med. 2004;351:2384–91. [PubMed]
118. Furukawa T, Koumi S, Sakakibara Y, et al. An analysis of lidocaine block of sodium current in isolated human atrial and ventricular myocytes. J Mol Cell Cardiol. 1995;27:831–46. [PubMed]
119. Ahmmed GU, Hisatome I, Kurata Y, et al. Analysis of moricizine block of sodium current in isolated guinea-pig atrial myocytes. Atrioventricular difference of moricizine block. Vascul Pharmacol. 2002;38:131–41. [PubMed]