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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Dev Dyn. Author manuscript; available in PMC 2009 September 29.
Published in final edited form as:
PMCID: PMC2754064
NIHMSID: NIHMS131428

Embryonic Chick Corneal Epithelium: A Model System for Exploring Cell-matrix Interactions

Abstract

In her initial research, Elizabeth D. Hay studied amphibian limb regeneration, but later switched her focus, and for the remainder of her career addressed the role of the extracellular matrix (ECM) in regulating embryonic morphogenesis. Much of that work used the embryonic chick corneal epithelial model. This review highlights many of the discoveries that she made using this model. Hay was the first to show that embryonic corneal epithelial cells produce fibrillar collagen. Her lab was among the first to demonstrate that corneal epithelial cells respond to a collagenous substrate by increasing ECM production, and that purified ECM molecules, added to cultures of epithelial sheets, induce a reorganization of the actin cytoskeleton. These data led to the first theories of cell-matrix interactions, illustrated in a ‘hands across the membrane’ sketch drawn by Hay. Recent work with the epithelial sheet model system has elucidated many of the signal transduction pathways required for actin reorganization in response to the ECM. In all, this body of work has amply supported Hay's belief that the embryonic corneal epithelium is a powerful model system for exploring the role of the ECM in regulating the cytoskeleton, in directing cell migration, and in profoundly influencing cell growth and differentiation during development.

Keywords: embryonic corneal epithelium, cell-matrix interactions, cytoskeleton, actin, extracellular matrix, signal transduction

Fibrillar Collagen Production by Epithelial Cells

Elizabeth D. Hay, called Betty, by all who knew her, was a pioneer in cell and developmental biology. She began her career investigating limb regeneration in salamanders, and when electron microscopy was being integrated with autoradiography, she was among the first to fruitfully apply them to study regeneration. In several early papers, Hay reported that both the salamander epithelial and fibroblast cells of regenerating limbs appeared to be producing, organizing, and assembling collagen matrices (Hay and Revel, 1963). At that time, the importance of the extracellular matrix (ECM) was just starting to be appreciated, and the word “collagen” referred to the only known collagen: fibrillar type I collagen. Hay's and Revel's conclusions about the synthesis of salamander epithelial type I collagen were based on morphological, “pulse chase” experiments. Systemic injection of tritiated proline demonstrated that, shortly after injection, 3H was localized within intracellular organelles, mainly the ER and the Golgi apparatus. However, at later time points, the label was found in the ECM underlying the epithelium, then referred to as the basement lamella (Hay and Revel, 1963). Publication of this work elicited substantial controversy, because it went against the current dogma of that time, which was that collagen (type I) was produced only by fibroblasts (Kallman and Grobstein, 1965; Bernfield, 1970).

Hay took the criticism as a personal challenge to prove that epithelial cells could synthesize ECM molecules, including type I collagen. To accomplish this she needed an epithelium that could be dissected free of other tissue types, and would survive sufficient lengths of time in culture to demonstrate collagen synthesis and assembly. The salamander limb epithelium was not a good candidate for in vitro studies because the tissue was not sterile and its biosynthetic levels were insufficient to address the core questions. Hay read with interest the paper published by the Coulombres, which contained exquisite electron micrographs of collagen in the developing chick cornea. The micrographs showed that the fibrils were secreted and assembled in orthogonal arrays, that is, the fibrils were arranged in mat-like layers, where within a layer the fibrils were unidirectional. Any given layer would have fibrils running at nearly a 90-degree angle with respect to the fibrils in the layer above it or below it (Coulombre and Coulmbre, 1958).

Embryonic Chick Corneal Epithelial Model System

Hay turned to the embryonic chick corneal model system because the organization of the matrix was similar to the pattern observed in the basement lamella of the salamander limb. The collagen fibrils first laid down by the corneal epithelium were assembled into an acellular “primary” stroma in the absence of fibroblasts. This occurred 2 days before the stroma was invaded by neural crest-derived cells, which synthesized the “secondary,” mature stroma. These features made the embryonic chick cornea a perfect model for her subsequent experiments.

By the mid 1960s, Jean Paul Revel and Hay had completed a comprehensive study of the fine structure of the developing chick cornea. Their results were published in a monograph that is referenced to this day (Hay and Revel, 1969). The early development of the cornea was described, carefully documenting the attributes of the epithelial-derived primary stroma before fibroblast invasion. The fibrils made by the epithelium had a periodicity of approximately 600Å, with an interband striation pattern identical to fibrils assembled in the secondary corneal stroma, synthesized by the fibroblasts. At the time, this research provided the most compelling evidence that epithelial cells produced the collagenous primary stroma. The discovery of a second collagen, type II (Miller and Matukas, 1969), was still 2 years away. If Hay's embryonic epithelial fibrillar collagen papers had been published after the discovery of type IV collagen, she would still have been likely to face skepticism, because type IV would have been considered “the” epithelial collagen. Because her observations were novel, they were bound to create discord. No matter. Hay's persistence in her research, along with subsequent work by others, has supported her discovery that embryonic chick corneal epithelia synthesize fibrillar collagens. It is now known that this epithelium can secrete a minimum of three fibrillar collagens: type I, type II (Hendrix et al., 1982), and type V (Gordon et al., 1994).

Influence of Matrix on the Embryonic Chick Corneal Epithelium

The next series of experiments using cultured corneal epithelia launched a body of work that has spanned 40 years and remains an active field to this day. James W. Dodson joined Hay's laboratory as a postdoctoral fellow in the late 1960s. He had grown chick skin in organ culture on a variety of “cell-free” substrata (Dodson, 1967). These substrata were made by dissecting an organ known to have an abundant matrix and killing its cells with low temperature, leaving the matrix intact. Dodson was quick to apply this approach to the corneal epithelium. By culturing sheets of corneal epithelia on “cell-free” lens capsules (Fig. 1A), he demonstrated that the epithelium produced a proline rich ECM, that was absent when the same cells were cultured on plastic. This was additional evidence for collagen synthesis by epithelia (Dodson and Hay, 1971). It also suggested to Hay that the components of the ECM might regulate embryonic growth and differentiation. Her group exploited a combination of morphological and autoradiographic approaches to examine the response of corneal epithelia to various substrata (Hay and Dodson, 1973; Dodson and Hay, 1974). Stephen Meier, a graduate of Michael Solursh's laboratory, joined the Hay lab in 1972. His background in chondrogenesis included cell–matrix interactions, and he brought biochemical analytical skills to the project. His work provided the initial quantitation of collagen synthesis (Meier and Hay, 1974b) and glycosaminoglycan (GAGs) synthesis (Meier and Hay, 1973) induced in corneal epithelia in response to specific substrata. From this work, it became evident that an appropriate substratum was required for the embryonic epithelium to synthesize and assemble a primary stroma in vitro.

Fig. 1
The culture system developed by Dodson, Meier, and Hay placing epithelium on killed lens capsule. A: Epithelium cultured directly on lens capsule. B: Epithelium separated from lens capsule by a Nucleopore filter barrier. Reprinted from Meier and Hay, ...

Meier next designed a set of experiments to demonstrate that physical contact with the substratum enhanced the epithelial response. He observed that the basal surface of corneal epithelial sheets, enzymatically released from the 5-day chick embryo, contained protrusions termed “blebs.” Meier sandwiched Nucleopore filters between the epithelial sheet and killed lens capsule (Fig. 1B,C). When a single filter was used in the culture system (Meier and Hay, 1975), the basal blebs traversed the filter's pores and made contact with the lens capsule substratum (Fig. 1C). However, when more than one filter was interposed, the response to the lens capsule diminished in proportion to the number of filters (Fig. 1D). In addition, there was a direct relationship between the size of the pore and the response to the substrate (Meier and Hay, 1975). This was strong evidence that cell contact with the substratum was required for stimulating the epithelial cells to synthesize collagen. Not only did these experiments reinforce the concept that the developing corneal epithelium needed attachment to ECM to induce synthesis of the primary stroma, but in their 1975 papers Meier and Hay proposed that epithelial cell processes might contain specific surface sites for collagen-ECM interaction (Meier and Hay, 1975), foreshadowing the discovery of integrins.

Matrix Induced Actin Reorganization

During the same time period, Richard Hynes, while exploring the differences between normal and tumor cells, identified and isolated a large molecular weight protein that bound to normal cells (Hynes, 1973). Hynes termed the protein LETS (large, external transformation-sensitive) protein. It was later proven to be a ubiquitous extracellular protein, and renamed fibronectin (Wartiovaara et al., 1974; Mautner et al., 1977). In the latter part of the 1970s, several laboratories demonstrated that extracellular bundles of fibronectin, attached to the basal surface of epithelial cells, were aligned with the submembranous actin filaments in the cytoplasm (Hynes et al., 1978; Singer, 1979; Heggeness et al., 1978). Additional work showed that fibronectin was able to induce the rearrangement of actin filaments in fibroblasts and myoblasts (Ali et al., 1978). The term “fibronexus” was coined to describe this conjoined extra- and intracellular structural complex (Singer, 1979).

Stephen Sugrue joined Hay's lab in the late 1970s to determine whether purified ECM proteins, such as specific collagen types (I, II, and IV), laminin and fibronectin, would promote the synthesis of differentiation markers by corneal epithelium. Sugrue's data demonstrated that epithelia grown on Millipore filters, with no soluble matrix component added to the culture, continued to bleb for the duration of the experiments (12–14 hr; Fig. 2A). No fibrillar collagen was synthesized. However, if soluble collagen (COLL), laminin (LAM), or fibronectin (FN) were added to the medium, blebs were withdrawn within 2–6 hr and the basal cell surfaces flattened (Table 1; Fig. 2B). Sugrue and Hay demonstrated that soluble ECM molecules added to the medium bound to the basal epithelial surface, and these induced and regulated collagen synthesis. In addition, it was shown that the binding sites for the soluble matrix components were proteinaceous (Sugrue and Hay, 1986). In contrast, epithelia grown on filters in the presence of added albumin, IgG, or glycosaminoglycans continued to form basal blebs, and did not attain the flattened basal phenotype (Table 1; Fig. 2A). Also, epithelia cultured on solid matrices, such as glass, continued to bleb in the absence of ECM components (Sugrue and Hay, 1981). It was clear that cell–ECM interactions were central to actin reorganization and expression of a differentiated phenotype. Ultimately, myosin S-1 decoration definitively established that the basal submembranous filament bundles were F-actin (Fig. 2C). Of interest, new protein synthesis was not required for the corneal epithelial cells to respond to collagen or laminin, but was needed for the cell's response to fibronectin (Sugrue and Hay, 1982). These data suggested that actin reorganization in response to the ECM was responsible for bleb retraction (Fig. 2B). The intracellular basal sheet occupied by actin was termed the “actin cortical mat” (ACM). Hay, who was very artistic, drew her interpretation of the interactions between the basal epithelia and the extracellular matrix in an illustration referred to by lab members as “hands across the membrane” (Fig. 2D). This drawing was used by the group for conceptual discussions, but was never published. It was given to Sugrue when he left Harvard because it represented a summary of his work. As a tribute to Hay, he framed the original drawing and presented it back to her at a dinner following the Matrix and Morphogenesis conference, held in Boston to honor her 75th birthday and her contributions to the field.

Fig. 2
Diagrams and electron micrographs illustrating the effects of different experimental conditions on the organization of the basal cell surface. A: The basal surface blebbed when the basal lamina was removed by EDTA or enzyme treatment, and the blebbing ...
Table 1
Summary of Stimulators and Inhibitors of Corneal Epithelial Actin Reorganizationa

Cell-Matrix Interactions

Because ECM binding to the cell surface clearly involves specific molecular interactions, a search for ways to block these interactions was begun in several labs. Once techniques were refined to purify ECM molecules, monoclonal and polyclonal antibodies were generated against whole molecules and their proteolytic fragments. The goal was to identify blocking antibodies that would inhibit cell adhesion to specific substrates. In the early 1980s, review papers highlighted the experimental data demonstrating evidence for integral membrane linkers (Fig. 3). Hay was an author on several of these reviews (Hay, 1977, 1981b, 1984; Hynes et al., 1982; Singer, 1979). Yet the identity of the transmembrane linker proteins proved elusive. Finally, in the mid 1980s the first integrin was identified and cloned (Tamkun et al., 1986); for review see (Hynes, 2004). As with many protein families, once the first integrin was cloned, the flood gates opened and many others were quickly identified. Hynes organized a Gordon Conference in early 1987, inviting everyone he knew who was working on cell adhesion proteins. By the end of that week, the integrin family was pieced together and a common terminology accepted (Hynes, 1987). In the late 1980s and early 1990s many groups were using the newly characterized integrin antibodies to identify tissue-specific subunits. The Trinkaus-Randall and Gipson labs were the first to describe integrin subunits in the corneal epithelial cells (Stepp et al., 1990a,b; Trinkaus-Randall et al., 1990; Grushkin-Lerner and Trinkaus-Randall, 1991; Stepp, 2006).

Fig. 3
Diagrams of several models depicting the possible relation of extracellular matrix (ECM) molecules to the cell surface. All models envision plasma membrane receptors for one or more molecules. CO, collagen; FN fibronectin; PG, proteoglycans; HA, hyaluronic ...

In 1982, Kathy Svoboda joined the Hay lab as a post doctoral fellow, being interested in the role of cytoskeletal reorganization during morphogenesis. Her goal was to determine whether actin reorganization in response to the ECM is a necessary prerequisite for increased collagen production within cultured corneal epithelia. Her data indicated that increased collagen synthesis was, indeed, actin dependent, and if the actin mat was disrupted, the rough endoplasmic reticulum became displaced from the basal cytoplasm (Svoboda and Hay, 1987).

Impact of Hay's Corneal Epithelial Research on Epithelial Cell Biology

For the most part in the late 1980s, Hay's work with the corneal epithelial sheet model was superseded by research on epithelial mesenchymal transitions. However, her impact on corneal epithelial cell biology continues to this day. For example, after leaving the Hay laboratory, Svoboda has continued to use the corneal epithelial sheets in her own laboratory, further characterizing binding of the cell sheets to soluble matrix, identifying the signal transduction pathways, and actin associated proteins involved in the reorganization of the actin cortical mat (Svoboda, 1990, 1992; Khoory et al., 1993; Hirsch et al., 1994; Svoboda et al., 1999a). To visualize the changes that Hay's group had been showing biochemically, confocal microscopy was refined as a tool to evaluate actin reorganization (Fig. 4). En face images with fluorescently labeled phalloidin showed bright fluorescent spots when the cells were optically sectioned transverse to the epithelial blebs (Fig. 4C), and large actin bundles formed in parallel on the base of the cells as the ACM was reorganized (Fig. 4D). This morphological method unraveled the three-dimensional organization of actin and its associated proteins, intracellular organelles and mRNAs for collagen I and actin. (Svoboda, 1991, 1992; Khoory et al., 1993; Yeh and Svoboda, 1993, 1994; Svoboda et al., 1999a). The time-course of binding was measured using fluorescein isothiocyante (FITC) -labeled fibronectin and collagen I (Fig. 5A,B). The corneal epithelial sheets needed 15 min in the presence of soluble matrix before the ACM began reforming, and the mat was complete by 2 hr (Fig. 5C). Binding and competition experiments also showed that binding was saturable (Fig. 5D). These experiments complemented both Meier and Sugrue's experiments on the corneal epithelial response to collagen (Meier and Hay, 1974a; Sugrue and Hay, 1981, 1982).

Fig. 4
Confocal microscopy of actin in corneal epithelial sheets. Schematic drawing of embryonic corneal epithelia isolation and culture. A: Epithelia were isolated as a sheet of tissue and placed on a polycarbonate filter, basal side down, then cultured at ...
Fig. 5
Confocal images of epithelia grown on filters with fluorescently labeled matrix components. A,B: Corneal epithelia were isolated without the basal lamina (−BL), incubated with media containing fluorescein isothiocyanate–fibronectin (FITC-FN) ...

Tyrosine phosphorylation of many signaling proteins was observed in corneal epithelia as they bound exogenous fibronectin or collagen, beginning within 15 min of exposure to the soluble matrix (Svoboda et al., 1999b). Focal adhesion kinase (FAK) and p190RhoGAP were tyrosine phosphorylated within 5 min. Other MAP kinase signaling pathways (Erk-1, 2) and PI-3 kinase were activated later (30–60 min). These phosphorylations were required for actin reorganization, as shown with a tyrosine and Src kinase inhibitor, Herbimycin A (Table 1; Svoboda et al., 1999b). Furthermore, blocking the MAP kinase and PI-3 kinase pathways with specific inhibitors also prevented actin reorganization and decreased ECM binding (Table 1) (Chu et al., 2000). Monolayer cultures of corneal epithelial cells grown on laminin, fibronectin and collagen I showed that ACM reorganization was accompanied by an increase in intracellular pH (Wu et al., 1995). Reorganization of the actin cortical mat in the epithelial sheets was Rho-dependent (Reenstra et al., 2002) and ROCK-dependent (Svoboda et al., 2004). All of this work has been derived from the elegantly simple conclusion of Elizabeth Hay that epithelial cells in the embryonic chick cornea can synthesize and assemble fibrillar collagen and do so in response to its unique ECM.

Some of Hay's other trainees have continued their research on the cornea. May Griffith, a former postdoctoral fellow, has developed corneal constructs, resulting in many publications, including one in Science (Griffith et al., 1999). Others, including Stephen Sugrue, expanded their interest to other epithelia. In Sugrue's case, this led to the discovery of the molecule pinin (Ouyang et al., 1997; Shi et al., 2000; Zimowska et al., 2003; Joo et al., 2005), a multifunctional molecule involved in cell–cell interactions and much more. Additionally, colleagues saw the potential of Hay's corneal model system: Ilene Gipson adapted the model to rabbit cornea (Geggel and Gipson, 1985; Gipson et al., 1985; Spurr and Gipson, 1985). This began a body of work unraveling the components of the adhesion complex between the epithelium and the stroma in the cornea.

Perspectives

This embryonic corneal epithelial organ culture model provides investigators with a more physiological system than monolayer cell cultures. Epithelial–stromal interactions, actin-associated protein changes, the influence of soluble matrix, and downstream signaling molecules, have all been studied using it. This model preserves many characteristics of in vivo epithelium, including apical-basal polarity and cell–cell junctions. Corneal epithelial cells have a rich integrin subset that also responds to ECM molecules (Table 1, Stepp, 2006). Powerful new techniques are emerging using fluorescently tagged proteins and real-time cell imaging that will enable a much better understanding of the intracellular events that take place when the epithelium is challenged by trauma, toxins or transforming agents.

Because signaling triggers alterations in cell metabolism, in gene expression and in cell behavior, continued use of the corneal epithelial model could link signal transduction cell biology with embryonic morphogenesis.

Acknowledgments

This review is dedicated to the memory of Elizabeth D. Hay. We thank Steve Sugrue for helpful insights and for providing the “hands across the membrane” illustration. We thank Developmental Biology and Journal of Cell Biology for permission to reprint figures. Dedication: K.K.S. and M.K.G. were funded by NIH/NEI.

Grant sponsor: NIH/NEI; Grant number: EY08886; Grant number: EY014389; Grant number: EY09056; Grant sponsor: UMDNJ/Rutgers Center; Grant number: AR055073.

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