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Appl Environ Microbiol. 2009 October; 75(19): 6110–6123.
Published online 2009 August 7. doi:  10.1128/AEM.00914-09
PMCID: PMC2753066

Transcriptomic Response of Escherichia coli O157:H7 to Oxidative Stress[down-pointing small open triangle]

Abstract

Chlorinated water is commonly used in industrial operations to wash and sanitize fresh-cut, minimally processed produce. Here we compared 42 human outbreak strains that represented nine distinct Escherichia coli O157:H7 genetic lineages (or clades) for their relative resistance to chlorine treatment. A quantitative measurement of resistance was made by comparing the extension of the lag phase during growth of each strain under exposure to sublethal concentrations of sodium hypochlorite in Luria-Bertani or brain heart infusion broth. Strains in clade 8 showed significantly (P < 0.05) higher resistance to chlorine than strains from other clades of E. coli O157:H7. To further explore how E. coli O157:H7 responds to oxidative stress at transcriptional levels, we analyzed the global gene expression profiles of two strains, TW14359 (clade 8; associated with the 2006 spinach outbreak) and Sakai (clade 1; associated with the 1996 radish sprout outbreak), under sodium hypochlorite or hydrogen peroxide treatment. We found over 380 genes were differentially expressed (more than twofold; P < 0.05) after exposure to low levels of chlorine or hydrogen peroxide. Significantly upregulated genes included several regulatory genes responsive to oxidative stress, genes encoding putative oxidoreductases, and genes associated with cysteine biosynthesis, iron-sulfur cluster assembly, and antibiotic resistance. Identification of E. coli O157:H7 strains with enhanced resistance to chlorine decontamination and analysis of their transcriptomic response to oxidative stress may improve our basic understanding of the survival strategy of this human enteric pathogen on fresh produce during minimal processing.

The epidemiology of food-borne disease has changed rapidly over the past two decades. Recent surveys have shown that fresh produce caused over 28,000 cases of food-borne illness and has become the second-most-common food vehicle linked to outbreaks in the United States (23, 61, 77). Bacterial pathogens that have been the agents of gastrointestinal (GI) illness in fresh produce-associated epidemics include Salmonella enterica, pathogenic Escherichia coli, Shigella spp., Campylobacter spp., Listeria monocytogenes, Staphylococcus aureus, and Bacillus cereus (23, 61, 77). Among these, pathogenic E. coli caused 48% of the outbreaks associated with leafy vegetables. In particular, Shiga toxin-producing E. coli O157:H7, the leading causative agent of bloody diarrhea and hemolytic-uremic syndrome, was responsible for the highest proportion (21%) of all produce-linked outbreaks in the United States (19, 23, 61, 77). Recent fresh produce-linked E. coli O157:H7 outbreaks also showed a trend of increased severity of infections among susceptible populations over widely spread geographic locations (49). For instance, in the 2006 outbreak associated with packaged baby spinach, which involved about 200 cases of infection in over 26 states, more than 30 cases of hemolytic-uremic syndrome and three deaths were reported (12).

Wash water disinfection is commonly used to reduce microbial populations on fresh-cut produce and increase shelf life (8). Chlorinated water washes generally result in 1 to 2 log reductions in microbial load on the produce (8). Chlorine (i.e., sodium hypochlorite solutions) and other chlorine-based disinfectants may act as oxidants to disrupt cellular functions, including electron transport systems, DNA replication, metabolic enzymes, and membrane structure. However, a large organic load in wash water, which may be caused by the presence of soil and other organic matter, can decrease the oxidation reduction potential (ORP) and hence reduce the disinfecting efficacy of the chlorine wash water. As a consequence, some E. coli O157:H7 cells may survive in the product and subsequently cause human disease (10, 16, 42, 44, 46, 69, 73). It is also possible that bacterial cells can adapt to sublethal oxidative stress and initiate cellular defense mechanisms that may afford protection against subsequent stress conditions during infection in the human GI tract (65, 66, 86).

E. coli O157:H7 is a rapidly evolving pathogen that is highly adaptive to different ecological niches and environmental stresses (43), and large differences may exist among strains in their ability to survive stressful conditions. Such diverse phenotypes are likely to be the result of the organism's highly plastic and adaptive genome (35, 74, 83). E. coli O157:H7 strains associated with human disease outbreaks have been grouped into nine distinct phylogenetic clades (i.e., clades 1 through 9) (49), which display variable resistance to acid exposure in a simulated GI system (7). A microarray study of E. coli O157:H7 strain ATCC 43894 revealed that the transcription of the genes involved in oxidative stress was significantly upregulated after attachment of the pathogen to intestinal epithelial HT-29 cells (36).

In this study, we aimed to evaluate variations in chlorine resistance among E. coli O157:H7 strains implicated in food-borne illness outbreaks. We also explored the global gene transcriptional response of two chlorine-resistant E. coli O157:H7 strains (i.e., TW14359 and Sakai) under oxidative stress by DNA chip analysis. TW14359 was isolated from a human infected in the 2006 spinach outbreak in the United States (49). Sakai was isolated from a patient infected in the 1996 radish sprout outbreak in Japan, and its genome has been fully sequenced and annotated (25). This is the first study to report the strain- or clade-specific variations in chlorine resistance among E. coli O157:H7 strains and the first to characterize genome expression profiles of E. coli O157:H7 strains associated with fresh produce outbreaks under oxidative stress.

MATERIALS AND METHODS

Bacterial strains.

Forty-two E. coli O157:H7 strains were selected from a collection of over 500 human outbreak-associated strains to represent the nine previously identified clades of this pathogen (Table (Table1).1). All were compared in growth studies to assess relative resistance to chlorine. In addition, two strains were used for genome expression analysis, the fully sequenced reference strain Sakai (clade 1) (25) and TW14359 (clade 8), a strain collected from the 2006 multistate spinach outbreak (49).

TABLE 1.
Forty-two E. coli O157:H7 strains used in this study

Growth studies and chlorine resistance determination.

For growth studies, a single colony of each of the 42 strains was inoculated into 5 ml brain heart infusion (BHI) broth (Difco, Detroit, MI), and the broth cultures were incubated at 37°C with aeration for 24 h. The bacteria were then harvested by centrifugation at 4,000 × g for 10 min at room temperature and washed with 10 ml Butterfield's phosphate buffer. The cell density was adjusted in Butterfield's phosphate buffer to an optical density at 600 nm (OD600) of 0.8 using a Spectronic 21 spectrophotometer, and a 1:10,000 dilution was made into broth medium with or without 13% sodium hypochlorite (Acros Organics, Morris Plains, NJ) at selected concentrations. Growth of all strains was tested in Luria-Bertani (LB) broth (Difco); in addition, growth of strains Sakai and TW14359 was assessed in BHI broth. The oxidation-reduction potential of BHI or LB broth supplemented with 5 μl/ml of chlorine was determined using a Jenco pH/ORP/temperature controller (Jenco Electronics, San Diego, CA) according to the manufacturer's instructions.

The Bioscreen C automatic microbiology growth curve analysis system (Growth Curves, Piscataway, NJ) was used to monitor the growth of the diluted cultures. An aliquot of 200 μl of each culture was loaded in triplicate into a Bioscreen C honeycomb plate, which was preloaded with 200 μl of the same medium. The instrument was programmed to measure cell culture turbidity (OD600) every 5 min for 18 h at 37°C with continuous shaking. For each experiment, sterile LB broth was used as a negative control.

Turbidity data were exported to Microsoft Excel to generate bacterial growth curves. The lag phase of the growth was defined as the time (in hours) between the initial inoculation and the time point when cell turbidity reached an OD600 of 0.2. The extended lag phase was calculated as the difference (in hours) in the lag phase of a strain grown in the presence of chlorine minus that of the same strain grown without chlorine. All growth experiments were repeated at least five times on different days. Statistical analysis was conducted using a one-way analysis of variance and Tukey's studentized range statistical test.

Oxidative stress, RNA isolation, and DNA chip hybridization.

A 1-ml aliquot each of Sakai and TW14359 overnight cultures was inoculated into 9 ml BHI broth and allowed to grow at 37°C for 4 h to reach early exponential phase. For oxidative stress, cells were washed with buffered peptone water (0.1%) at room temperature and incubated in 10 ml BHI broth supplemented with 30 μl sodium hypochlorite stock solution (13%) or 1.1 μl hydrogen peroxide solution (30%) at 37°C for 30 min. A 5-ml volume of each culture was then mixed with 10 ml RNAProtect (Qiagen, Valencia, CA), vortexed, incubated at room temperature for 5 min, and centrifuged at 3,220 × g for 15 min to pellet the cells, which were then resuspended in 200 μl lysis buffer (30 mM Tris·Cl, 1 mM EDTA, pH 8.0, 15 mg/ml lysozyme, 10 μl proteinase K). Total bacterial RNA was extracted using the RNeasy Midi kit (Qiagen) according to the manufacturer's instructions. The RNA quantity and integrity were analyzed using an Agilent 2100 bioanalyzer (Agilent Technologies, Santa Clara, CA). The E. coli genome 2.0 array was used in this study. The cDNA synthesis, labeling, and hybridization were performed at the University of Chicago Functional Genomics Facility (Chicago, IL), following the Affymetrix GeneChip expression analysis protocols (Santa Clara, CA). Briefly, the arrays were hybridized for 16 h at 45°C and 60 rpm in an Affymetrix hybridization oven 640, washed, stained with streptavidin-phycoerythrin in an Affymetrix fluidics station 450, and scanned using the Affymetrix GeneChip scanner 3000 7G. All experiments were repeated at least two times on different days.

DNA chip data analysis.

The acquisition and initial quantification of array images were performed using the GCOS program (Affymetrix). We used the statistical expression algorithm in GCOS to compute three types of detection call (i.e., present, absent, or marginal) after hybridization with this array. Affymetrix raw data were normalized per chip and per gene and filtered based on raw signal intensity and detection call. A Pearson correlation coefficient (r value) was calculated to assess the reproducibility of duplicate chip data sets (P. Wessa, Free Statistics software version 1.1.23-r3, Office for Research, Development and Education [http://www.wessa.net]). Subsequent data comparison was performed using GeneSpring GS.7.3.1 (Agilent Technologies). Briefly, normalized signal intensities of each gene on replicate chips were averaged, converted to log 2 values, and compared between experiments (see Fig. Fig.2).2). The Benjamini-Hochberg false discovery rate test was performed along with the t test to filter differentially expressed genes (P < 0.05) with an expression fold change of ≥2 between a treatment and a control. Hierarchical clustering analysis was performed using the MultiExperiment Viewer module of the TM4 microarray software suite (67). The mean signal intensities of each gene on replicate chips were also compared between treatments in fold changes (see Table Table55).

FIG. 2.
Heat maps comparing the expression profiles of 284 stress-related genes in Sakai (SK) and TW14359 (TW) under chlorine and hydrogen peroxide treatments. Genes that are functionally associated with bacterial stress response were chosen for hierarchical ...
TABLE 5.
Differentially regulated genes in E. coli strains Sakai and TW14359 under chlorine or hydrogen peroxide treatment

qRT-PCR.

Ten genes that showed significant (P < 0.05) upregulation or downregulation in the microarray experiments were selected for analysis by quantitative real-time reverse transcription-PCR (qRT-PCR), along with two genes known to be associated with bacterial oxidative stress response (oxyR and soxR) as references. Forward and reverse PCR primers (Table (Table2)2) were designed using Primer3 software to produce an amplicon size of approximately 150 to 200 bp for each gene (63). RNA samples were extracted as described above and treated with DNase I (Invitrogen) to eliminate genomic DNA contamination. qRT-PCR was performed in a LightCycler 480 (Roche Applied Science) with cDNA reversely transcribed from 1 μg of purified total RNA using the Transcriptor first-strand cDNA synthesis kit (Roche Applied Science, Indianapolis, IN) and 60 μM of random hexamers supplied with the kit. qRT-PCR was run for 45 cycles with a mix of 5 μl cDNA, 10 μl 2× SYBR green PCR master mix (Roche Applied Science), and 1 μl each of forward and reverse primers (10 μM stock). For each reaction, both a negative control (PCR-grade water) and an RNA sample without reverse transcriptase were included to detect potential genomic DNA contamination. The relative expression fold change was calculated using a method described by Pfaffl (57). Briefly, the 16S rRNA gene was used as an internal reference for data normalization. Crossing point (Cp) cycles versus cDNA input concentrations were plotted to calculate the slope and the corresponding qRT-PCR efficiencies. The expression (E) fold change for each gene was then calculated using the following equation: ratio = EtargetΔCp target/ErefΔCp ref, where “ref” refers to 16S rRNA, “target” refers to genes to be tested, and ΔCp equals control Cp minus treatment Cp. The average log2 values and standard deviation from six RNA samples were reported for each experiment for statistical analysis.

TABLE 2.
Primer pairs used for qRT-PCR in this study

Data accession number.

The DNA chip data from this study were deposited in the NCBI Gene Expression Omnibus under accession number GSE15533.

RESULTS

Variations in chlorine resistance among 42 E. coli O157:H7 strains.

We used the extended lag phase (in hours) of E. coli O157:H7 cells under the sodium hypochlorite (chlorine) treatment in LB broth to reflect strain resistance to chlorine. A shorter lag phase indicated relatively higher chlorine resistance of a strain. When grown in LB broth without chlorine or treated with less than 3.0 μl/ml chlorine, all 42 E. coli O157:H7 strains showed a similar lag phase of approximately 3 h. In contrast, the lag phase of E. coli O157:H7 strains extended to 6 to 11 h when treated with 5.0 μl/ml chlorine in LB broth. All strains showed reduced cell turbidity at stationary phase at higher chlorine concentrations. Figure Figure11 is a box plot that shows, from the bottom to the top of each box, the minimum, first quartile, median, third quartile, and maximum values of extended lag phases of all 42 strains grouped by phylogenetic clade. For the purpose of statistical confidence and an estimation of data variations, each box includes data points from at least five independent experiments for each strain in that clade. For Tukey's statistical analysis, we combined clade 4, which had only one representative strain, with the strains of clade 3 in the analysis. Results of Tukey's test divided the 42 strains into four groups (A to D) based on the statistical differences of the average extended lag phase among the nine clades (Table (Table3).3). It is noticeable that strains from clade 6 were the least resistant to the chlorine treatment, whereas strains from clade 5 and clade 8 were the most resistant under the tested condition. In particular, the average extended lag phase of the strains in clade 8 (7.70 h) was significantly shorter than that of most other clades (Table (Table3).3). This result indicated that the strains in this clade exhibited higher chlorine resistance than strains from other clades of E. coli O157:H7. Interestingly, the two strains associated with fresh produce outbreaks, Sakai and TW14359 (25, 49), both displayed enhanced chlorine resistance in their respective clades. We tested their relative resistance under three additional concentrations of chlorine in BHI broth (i.e., 1.5, 3.0, and 4.0 μl/ml chlorine in BHI broth). When the chlorine concentration reached 4.0 μl/ml or higher, Sakai displayed a longer lag phase than TW14539, indicating that TW14539 is slightly more resistant to chlorine than Sakai (see Fig. S1 in the supplemental material).

FIG. 1.
Box plot of extended lag phase of 42 E. coli O157:H7 strains under chlorine treatment. The chlorine concentration was made at 5.0 μl sodium hypochlorite stock solution per 1 ml LB broth. Boxes represent the 25th to the 75th percentile of extended ...
TABLE 3.
Chlorine resistance as measured by extended lag phase among strains of the nine different E. coli O157:H7 clades

Global gene expression profiles of Sakai and TW14359 under oxidative stress.

Chlorinated water and hydrogen peroxide solutions are often used in the food industry for decontaminating foods and food contact surfaces. To explore the cellular response of E. coli O157:H7 to these oxidative sanitizers, we analyzed the transcriptomic profiles of both Sakai and TW14359 treated with sublethal concentrations of chlorine or H2O2. For the chlorine treatment, we used 3.0 μl/ml sodium hypochlorite in BHI broth because this concentration led to an extended lag phase in both Sakai and TW14359 (see Fig. S1 in the supplemental material). For the convenience of data analysis and interlaboratory comparison, we used the commercially available GeneChip E. coli genome 2.0 array (Affymetrix). This DNA chip includes 10,208 oligonucleotide probe sets that target 20,336 genes in four E. coli genomes, including E. coli K-12 (MG1655), uropathogenic E. coli CFT073, and two E. coli O157:H7 strains, Sakai and EDL933 (25, 56). A total of 8,006 probe sets (78.4% of all probes on this array) were called present or marginal in our chip hybridization and therefore were used for subsequent data analysis. About 21.6% of the probe sets on this array were not detected in at least one transcriptome and were excluded from analysis. Two duplicate chips were used for each of the following six experimental conditions: Sakai control (without treatment), Sakai treated with chlorine, Sakai treated with H2O2, TW14359 control (without treatment), TW14359 treated with chlorine, and TW14359 treated with H2O2. The reproducibility of duplicate chip data sets was evaluated using Pearson correlation analysis (Table (Table4).4). Pearson correlation values (r) ranged from 0.866 to 0.987, indicating high reproducibility between duplicate chips under all experimental conditions. It is noticeable that lower statistical correlations were found between the duplicate chips under the H2O2 treatment for both Sakai and TW14359.

TABLE 4.
Pearson correlation coefficients between DNA chip data sets

A total of 240 genes (4.57% of all genes) in the Sakai genome and 140 genes in the TW14359 genome were either upregulated or downregulated by at least twofold (P ≤ 0.05) under the chlorine treatment. In this study, we were particularly interested in examining differentially expressed genes with their functional annotations directly or indirectly related to bacterial oxidative stress. We divided all differentially expressed genes into six functional groups (A to F) and compared gene expression patterns under chlorine and H2O2 treatments in each group by a hierarchical clustering algorithm (Fig. 2A through F). It is an interesting observation that the overall transcriptional patterns of these oxidative stress-related genes in both Sakai and TW14359 strains appeared to be similar under the same treatments. However, the transcriptional patterns differed drastically between the chlorine and H2O2 treatments. About 68% of the genes known to be responsive to environmental stress, including those affected by oxidative damage, were upregulated by chlorine (Fig. (Fig.2A).2A). Approximately 69% of the annotated genes involved in Fe-S cluster assembly and repair, the thiol-redox system, and Fe-S cluster-containing proteins were also induced (Fig. (Fig.2B).2B). In addition, a majority of the genes involved in cysteine biosynthesis and transport were upregulated at different levels (Fig. (Fig.2C),2C), whereas genes encoding cold shock proteins, outer membrane proteins, and transporters were not affected by treatment with chlorine or H2O2 (Fig. 2D and E). No significant fold change was observed for known virulence genes in E. coli O157:H7, including the LEE operon and genes encoding the type III secretion system and Shiga toxins (Fig. (Fig.2F).2F). However, a few genes involved in bacterial biofilm formation and regulation were found to be upregulated. As we expected, chlorine or H2O2 caused a general reduction in the expression of genes encoding ribosomal proteins and amino acid transporters and genes involved in nucleotide synthesis, fatty acids, and lipoprotein metabolism.

Upregulated genes under oxidative stress.

One hundred thirty-three genes that were found to be differentially regulated in E. coli O157:H7 under either chlorine or H2O2 treatment are listed in Table Table5.5. We found that a number of stress-related regulatory genes and operons were induced under oxidative stress. For instance, the frmRAB operon (encoding an E. coli formaldehyde detoxification system) and a multiple antibiotic resistance operon (marRAB) were significantly induced by the chlorine treatment. Genes encoding two periplasmic repressors that interact with CpxA were upregulated by more than threefold in the presence of chlorine. uspA, which encodes a universal stress protein, was upregulated by 1.7-fold. One of the uspA paralogues, ydaA (or uspE), was also slightly induced. uspE can be induced by a variety of stresses which cause growth arrest of bacterial cells (22). Other regulatory genes, such as spy, yedW, and dps, were also upregulated by either chlorine or H2O2 treatment. The coordinate expression of the Pho regulon controls the cellular response to phosphate limitation in E. coli (62), and some of these genes, including phoU, phoBR, pstSCAB, and the alkaline phosphatase gene phoA, were upregulated by 1.5-fold to twofold in the presence of chlorine. Upregulation was evident only in phoP and not phoQ. The E. coli central stress regulator gene rpoS was slightly induced upon the treatments, as was the rprA gene, which encodes a protein that positively regulates RpoS translation.

The expression of two global regulatory genes in bacterial oxidative defense, soxR and oxyR, did not change during either of the oxidative treatments, whereas the OxyR regulons, including superoxide dismutase (SOD) genes sodABC, catalase genes katE and katG, and alkyl hydroperoxide reductases genes ahpF and ahpC, were moderately upregulated. Among the genes directly related to oxidative stress, we found that the genes in the ykgCIB operon were significantly upregulated by chlorine treatment. Gene ykgC was annotated as a putative oxidoreductase with FAD/NAD(P) binding and a dimerization domain (9). To date, there are no published data to confirm its biological function. We speculated that it might be involved in repairing the oxidative damage caused by chlorine. Genes yeeE and yeeD of the yeeDEF operon were induced by four- to fivefold in this study. Zheng et al. reported that yeeD was induced by hydrogen peroxide in both wild-type E. coli and its oxyR mutant, indicating that the oxidative response of this gene to H2O2 is OxyR independent (85). We also observed upregulation of the nrdHIEF operon by approximately twofold in the presence of chlorine and by threefold to fourfold under the H2O2 treatment. A few other genes encoding oxidoreductases were also identified to be upregulated.

Oxidative agents target the Fe-S clusters which serve as enzymatic cofactors in a number of cellular proteins. The genes involved in Fe-S cluster assembly and repair, such as the iron-sulfur cluster (iscSRUA-hscBA-fdx) and sufABCDSE operons, were upregulated as we expected (Table (Table5).5). Genes in the E. coli thioredoxin and glutathione pathway, which play an important role in detoxification during oxidative stress, including grxA, gshA, and gshB, were also marginally induced by the treatment with chlorine and H2O2. It is clear that the genes involved in cysteine synthesis and transport were significantly upregulated upon the oxidant treatments, possibly because this amino acid residue plays a critical role in the Fe-S cluster structure and thiol-redox systems. The cbl gene, which encodes a DNA-binding transcriptional activator of cysteine biosynthesis, was induced significantly by chlorine treatment. The H2O2 treatment also induced cbl expression in both strains. The expression of a periplasmic cysteine binding protein gene, fliY, was induced under both chlorine and H2O2 treatments. Interestingly, fliA, which encodes an RNA polymerase (σ28 factor), and fliZ (a regulator of fliA) in the same fliAZY operon showed poor hybridization on the chip and also no significant difference in transcription in the presence or absence of chlorine or H2O2. The pepB gene was also upregulated, which encodes a cysteinylglycinase responsible for dehydration of serine and threonine (51). The expression of more than 10 genes encoding heat shock proteins was increased in the presence of chlorine, in addition to genes ycfR and ybiM, which function in the regulation of biofilm formation. We also found induction of some osmotically inducible genes, genes encoding protein transporters, and some hypothetical genes.

qRT-PCR validation of DNA microarray results.

qRT-PCR analysis for 12 genes (6 upregulated, 4 downregulated, and 2 known oxidative stress response genes) was used to validate the DNA chip data in this study. As shown in Fig. Fig.3,3, real-time PCR data correlated well with the chip expression data (R2 = 0.87). For several genes, the fold change detected by real-time PCR appeared to be higher than that detected by DNA chip. This is not uncommon, considering that the DNA chip is generally less sensitive than qRT-PCR for quantification of gene expression (37). For instance, ycfR, a hypothetical protein related to stress response and biofilm formation, was shown to be >10-fold upregulated by the DNA chip and >100-fold upreguled by qRT-PCR. Surprisingly, the soxRS operon and oxyR, which are known to be responsive to oxidative stress in E. coli, did not show significant upregulation after a 30-min exposure to chlorine or H2O2 in either DNA chip or qRT-PCR experiments. We speculated that soxRS and oxyR probably respond to oxidative stress in much shorter time frames. For this reason, we tested the expression levels of soxR and oxyR after 10 min of chlorine treatment. Results showed that both soxR and oxyR were upregulated by twofold and 1.5-fold in Sakai and TW14359, respectively.

FIG. 3.
Correlation of gene expression fold change generated by the DNA chip and qRT-PCR for 12 selected genes. The fold change value was converted to the log2 values for comparison.

DISCUSSION

Different effects of hydrogen peroxide and sodium hypochlorite on E. coli O157:H7 growth.

The exposure of E. coli O157:H7 cells to H2O2 or chlorine for 30 min resulted in different transcriptomic profiles, as shown in Fig. Fig.22 and Table Table5.5. In response to the treatments with chlorine or H2O2, Sakai and TW14359 demonstrated similar transcriptional patterns, which is consistent with the observation that the two strains exhibited similar growth patterns under these oxidative conditions. However, for both Sakai and TW14359, a higher number of genes underwent significant changes in expression in response to chlorine than to hydrogen peroxide, which may be due to the lower molarities of H2O2 (1 mM) than chlorine (12 mM) used in the treatments. It is also possible that the different cellular effects of these two oxidative agents were caused by the differences in their reaction rates with amino acid residues in the proteins. For instance, the reaction rate of H2O2 with free thiol groups in the Cys residues is about 2.9 M−1 s−1 at pH 7.4 to 7.6, whereas the active chlorine OCl reacts with thiol groups at an extremely high rate (3.0 × 107 M−1 s−1) at pH 7.4 (30). Besides Cys residues, chlorine also exhibits high reaction rates with other amino acid side chains (55), which enables it to easily oxidize protein residues that are surface exposed or transiently accessible, leading to more profound oxidative damage.

It is worth mentioning that the freshness of sodium hypochlorite solution (chlorine) had a direct impact on the growth and extended lag phase of E. coli O157:H7 strains in this study. Different broth media (i.e., LB and BHI) also showed different capacities to buffer or neutralize free chlorine (ClO) under the incubation conditions. Based on our results, the ORP of chlorine decreased 49% and 29% in BHI and LB media, respectively, compared to those in water solution. However, we found that E. coli O157:H7 strains grown in BHI broth supplemented with fresh chlorine solution (see Fig. S1 in the supplemental material) actually displayed longer extended lag phases than those grown in LB broth supplemented with properly stored, 2-year-old chlorine stock solution (Fig. (Fig.1).1). This was probably a result of disproportionation of free chlorine (ClO) and therefore reduced bactericidal activity in the solution during extended storage (15, 64, 76).

Genes related to oxidative stress.

The global regulatory system soxRS is known to mediate the survival of E. coli under superoxide radicals. SoxR is a [2Fe-2S]-containing transcriptional regulator which activates transcription of the soxS gene in response to oxidative stress. SoxS in turn activates other genes of this regulon (79). In our study, an induction of soxR was evident after 10 min of exposure to chlorine, but elevated expression of this gene was not observed after 30 min of chlorine treatment. It appears that this regulatory system senses and responds to oxidation very rapidly in order to maintain a steady cellular growth. Another reasonable explanation could be that the effective oxidation was gradually neutralized by BHI broth after 10 min of incubation. H2O2 has a higher oxidative potential than chlorine, and the superoxide radicals generated by H2O2 may directly trigger the activation of soxS via SoxR, which functions as a transient sensor molecule for environmental stress.

The E. coli marRAB operon responds to a wide variety of compounds, including antibiotics, oxidative agents, and organic solvents (2). Induction of marRAB results in enhanced resistance to oxidative agents. Genes regulated by marRAB overlap significantly with those in the soxRS regulon, which may result from the structural similarity between the MarA and SoxS proteins and their DNA-binding sites. These two sets of regulons are modulated differently: the soxS gene is under redox regulation and positively controls SoxR, whereas marA is under negative control by MarR (1). During the chlorine treatment, the marRAB operon was significantly induced, while no change was observed in the soxRS operon. In contrast, hydrogen peroxide upregulated the soxS gene in Sakai and TW14359 by two- to threefold, whereas the marRAB operon was only slightly induced. These findings suggest that the expression of the marRAB and soxRS regulons is moderated through different regulatory circuits by chlorine and H2O2.

It has been reported that the exposure of E. coli cells to low levels of H2O2 can induce resistance to a subsequent lethal dose of this oxidative reagent and upregulate a number of genes governed by the regulator OxyR (14). E. coli produces catalases (KatE and KatG) to convert hydrogen peroxide to oxygen and water (72). The major function of catalases is to remove toxic byproducts of metabolism and reduce oxidative stress. OxyR is a positive regulator of catalases (14). The transcription of katE was induced slightly by the chlorine treatment, while the H2O2 treatment upregulated katG. To overcome the toxic effects of reactive oxygen species, E. coli has evolved three main classes of SOD: SodA, SodB, and SodC, which catalyze the disproportionation of superoxide to hydrogen peroxide and water (31). However, the transcriptional levels of SOD and catalase genes in exponential-phase cultures treated with chlorine and H2O2 were only marginally higher than those grown in BHI broth. Whether SOD or catalase would change its expression in the stationary phase in response to the oxidative agents has not been tested. A previous study showed that the expression of SOD or catalase increased significantly in Vibrio spp. at high temperatures during stationary phase, but no change in expression was observed in response to H2O2 (52).

The frmRAB operon was dramatically induced by chlorine treatment. This operon encodes a complete pathway for the degradation of formaldehyde in E. coli. It was found that the frmR gene was induced by formaldehyde 215-fold over the levels of uninduced cells (28). Bacteria endogenously produce formaldehyde, which is an extremely reactive chemical that cross-links with proteins and nucleic acids. E. coli has evolved several repair mechanisms to prevent the deleterious effects of formaldehyde accumulation. Among these is a glutathione-dependent repair system, which can be found in most prokaryotes and all eukaryotes. FrmA (formaldehyde dehydrolase) and FrmB (S-formylglutathione hydrolase) are the key enzymes in the E. coli formaldehyde detoxification system, where formaldehyde spontaneously reacts with intracellular glutathione in its degradation pathway (21). Glutathione, a predominant cellular thiol compound, acts as a sacrificial protectant against chlorine oxidation (13). We speculate that the lower cellular glutathione concentration during chlorine treatment triggered the formaldehyde degradation pathway and consequently increased the expression of the frmRAB operon in E. coli O157:H7.

Among more than 30 two-component systems known in E. coli, the cpxRA regulon is a stress response system that responds to cell envelope damage by activating proteases and altering protein conformation (4, 58). Changes in pH, osmolarity, and copper concentration are among the activating factors of the Cpx pathway. In the current study, neither chlorine nor H2O2 caused any change in the transcription of the cpxRA operon. However, two periplasmic cpxRA repressors that are able to interact with CpxA were upregulated dramatically by chlorine or H2O2. It has been shown that the expression of cpxRA was significantly increased at the end of the exponential phase via the general stress response factor RpoS when the cells were under nutrient-limiting stress (17). Further investigations using cpxRA mutants of Sakai and TW1439 strains may provide more detailed information about whether this two-component system plays a role in the oxidative stress response. The expression of the envelope stress response gene spy is controlled by both two-component systems Cpx and Bae (60). The upregulation of the spy gene in our study might be due to membrane disruption caused by chlorine.

The transcription of another two-component system, yedWV, was upregulated by chlorine. Compared with the well-documented E. coli cpxRA regulon, very limited information is available for this regulatory system (29). Our results suggested that this system may function in cellular detoxification in the presence of oxidative agents. Downstream from the yedWV operon in the E. coli genome is the yedXYZ operon, which is oriented in the opposite direction. Chlorine exposure increased the expression of this operon, although the yedX gene from both Sakai and TW14359 strains exhibited poor hybridization with the microarray probes. YedY is a novel bacterial oxidoreductase in E. coli, anchored to the cell membrane by the transmembrane protein YedZ (48).

Upregulation of other stress-related genes, such as uspA, dps, and gst, was also evident in this study. UspA is a well-characterized universal stress protein (22). Dps, a starvation-inducible nonspecific DNA-binding protein, plays a major role in both gene expression and protection of DNA from oxidative damage (3). Moreover, Dps restricts iron uptake and sequesters intracellular iron during H2O2 stress to protect the cell from the Fenton reaction (54). Increased transcription of dps in response to oxidative stress may allow more Dps to preserve the supercoiled protective state of the chromosomal DNA. Glutathione S-transferase (GST) is involved in protection against oxidative stress in bacteria. An E. coli gst mutant was more sensitive to hydrogen peroxide than its wild-type parent strain (32). In our study, the transcription of gst was upregulated moderately in the presence of chlorine and H2O2. Expression of genes encoding YfcF and YfcG, which have GST-like activity in E. coli, did not change under these conditions. In bacteria, a number of enzymatic reactions in which O2 acts either as a catalyst or as a cofactor are driven by the redox properties of the thiol group of cysteines (78). E. coli contains thioredoxin and glutathione pathways to control the electron flow in the O2-dependent thiol reaction network. Chlorine treatment marginally upregulated the transcription of gshA, gshB, and grxA, which encode rate-limiting enzymes γ-glutamylcysteine synthetase GshA, glutathione synthetase GshB, and one of the three glutaredoxins, GrxA, in the glutathione pathway, respectively. There was no change observed in the expression of other components in this pathway or in the thioredoxin pathway.

When the bacterial cells were added to the chlorine-containing media, they were shifted to an osmotically stressful environment, even though the pH remained stable due to the buffering capacity of the growth media. The expression of chaA, which responds to changes in osmolarity and pH (75), was induced fourfold in both strains. Other osmotically inducible genes were also upregulated to allow cell integrity to be maintained in response to the change in osmotic pressure. E. coli contains two classes of ribonucleotide reductases (RNR), Ia and Ib. These enzymes catalyze the conversion of nucleotides to deoxynucleotides to provide DNA building blocks during DNA synthesis and repair (53). Class Ib enzymes are encoded in the nrdHIEF operon, which can be induced under oxidative stress and iron limitation (20). We observed upregulation of the nrdHIEF operon in the presence of chlorine and H2O2 treatment, while the genes encoding ribonucleotide reductase class Ia enzymes were induced only slightly.

Fe-S system and cysteine biosynthesis.

Under oxidative stress, the bacterial cells are exposed to high levels of oxygen species. The oxygen species convert the exposed Fe-S clusters, which are the most abundant and diversely employed enzymatic cofactors, to unstable forms that quickly decompose. As a result, Fe-S clusters are the most vulnerable targets for oxidative damage. The assembly of iron-sulfur clusters in E. coli involves the following two independent systems: ISC, encoded by iscRSUA-hscBA-fdx, and SUF, encoded by sufABCDSE (68). Both the isc and suf operons could be similarly induced by hydrogen peroxide (85). In the present study, both chlorine and H2O2 caused the induction of the genes in the isc operon by two- to threefold. Genes encoding other proteins in the Isc system, such as HscB, HscA, and Fdx, were also upregulated by approximately twofold. On the other hand, the suf operon sufABCDSE was slightly induced.

Cysteine is by far the most common amino acid residue acting as a thiolate ligand in the Fe-S clusters. Cys residues, existing in an alternative reduced thiol (SH) or oxidized disulfide bond (S-S) form, not only are major components in thiol-redox systems but also serve as conserved residues in numerous proteins. As expected, both chlorine and hydrogen peroxide treatments significantly upregulated the genes in cys biosynthesis, including cysCND, cysJIH, cysPUWAM, and cysZK.

Heat shock proteins.

The majority of heat shock proteins (Hsp) are molecular chaperones that bind to nascent, misfolded, or damaged polypeptides and assist them in reaching a native conformation (24). They play important roles in the bacterial stress response. The transcription of more than a dozen heat shock proteins was upregulated upon chlorine treatment in this study. Increased transcription of these genes may represent the cellular response to protein denaturation by the active oxygen species. The accumulation of these heat shock proteins may aid in repairing pressure damage. DnaK/DnaJ/GrpE constitute the primary chaperone machinery in E. coli (70). A recent study suggested that bleach can activate the bacterial heat shock protein hsp33 (also called hslO), and hence bacteria can protect essential cellular proteins against aggregation (81). It was also found that Hsp33 can be controlled by oxidative stress at the posttranslational level (82). We observed only moderate upregulation of hsp33 transcription in the current expression study. The treatment of hydrogen peroxide did not cause a significant change in heat shock gene transcription. This observation is consistent with the fact that H2O2 did not cause protein aggregation, although it possesses a higher redox potential than chlorine (81).

Other upregulated genes.

In E. coli K-12, the ycfR gene, which is induced under a variety of stress conditions, downregulates biofilm formation by altering cell surface hydrophobicity (6, 84, 85). The transcription of ycfR was upregulated by >10-fold under chlorine treatment and was slightly induced by H2O2 in both Sakai and TW14359 strains. Another gene, ybiM, which encodes a biofilm formation inhibitor, was also significantly upregulated. The results suggest that chlorine-treatment of E. coli O157:H7 cells may inhibit biofilm production. Two open reading frames (ORFs) of unknown function, ycfQ and ycfS, which are located upstream and downstream from ycfR in opposite directions, respectively, were also upregulated moderately.

Treatment with chlorine or hydrogen peroxide also induced a number of genes which, by annotation, are not directly related to oxidative stress. For example, the chlorine treatment greatly induced the transcription of the cynTSX operon, which encodes cyanase and cyanate permease. Expression of cynS and cynT can change the balance between bicarbonate and CO2 in E. coli (38), yet the relationship between the induction of the cynTSX operon and oxidative stress remains to be elucidated. In this study, we observed the upregulation of proP, which is involved in proline transport. It remains to be determined whether proP was induced because of osmotic or oxidative stress, considering that this gene can be upregulated by both of these stresses (5, 34, 40, 41, 45, 71). LysR is a positive regulator of lysA, which encodes the enzyme responsible for lysine synthesis. Sequence comparison revealed that E. coli LysR showed extensive homology to OxyR (82). In this study, the chlorine treatment that activated OxyR also might have activated LysR, and therefore lysA was significantly upregulated.

Through transcriptomic analysis of two chlorine-resistant E. coli O157:H7 strains associated with fresh produce outbreaks, we identified a list of genes that possibly mediate the oxidative stress resistance of this pathogen. The cellular response of E. coli O157:H7 to oxidation may involve complex interactions of proteins and a number of intrinsically linked metabolic pathways. Future studies of the functions of these newly identified genes and pathways may help us to better understand the physiology of E. coli O157:H7 and other related bacterial species under oxidative stress.

Supplementary Material

[Supplementary material]

Acknowledgments

We acknowledge the late Thomas Whittam at Michigan State University for providing the bacterial strains, John Greene at SRA International for helpful discussion on microarray data analysis, and James T. Riordan at Michigan State University for helpful discussions of the real-time PCR experiment. We also thank Diane Stewart and Xiaoqian Rui at the National Center for Food Safety and Technology for their technical assistance in this study.

This study was supported by a U.S. Food and Drug Administration cooperative agreement research grant to the National Center for Food Safety and Technology of the Illinois Institute of Technology.

Footnotes

[down-pointing small open triangle]Published ahead of print on 7 August 2009.

Supplemental material for this article may be found at http://aem.asm.org/.

REFERENCES

1. Alekshun, M. N., and S. B. Levy. 1999. Alteration of the repressor activity of MarR, the negative regulator of the Escherichia coli marRAB locus, by multiple chemicals in vitro. J. Bacteriol. 181:4669-4672. [PMC free article] [PubMed]
2. Alekshun, M. N., and S. B. Levy. 1997. Regulation of chromosomally mediated multiple antibiotic resistance: the mar regulon. Antimicrob. Agents Chemother. 41:2067-2075. [PMC free article] [PubMed]
3. Almirón, M., A. J. Link, D. Furlong, and R. Kolter. 1992. A novel DNA-binding protein with regulatory and protective roles in starved Escherichia coli. Genes Dev. 6:2646-2654. [PubMed]
4. Batchelor, E., D. Walthers, L. J. Kenney, and M. Goulian. 2005. The Escherichia coli CpxA-CpxR envelope stress response system regulates expression of the porins ompF and ompC. J. Bacteriol. 187:5723-5731. [PMC free article] [PubMed]
5. Bayer, A. S., S. N. Coulter, C. K. Stover, and W. R. Schwan. 1999. Impact of the high-affinity proline permease gene (putP) on the virulence of Staphylococcus aureus in experimental endocarditis. Infect. Immun. 67:740-744. [PMC free article] [PubMed]
6. Beloin, C., J. Valle, P. Latour-Lambert, P. Faure, M. Kzreminski, D. Balestrino, J. A. Haagensen, S. Molin, G. Prensier, B. Arbeille, and J. M. Ghigo. 2004. Global impact of mature biofilm lifestyle on Escherichia coli K-12 gene expression. Mol. Microbiol. 51:659-674. [PubMed]
7. Bergholz, T. M., and T. S. Whittam. 2007. Variation in acid resistance among enterohaemorrhagic Escherichia coli in a simulated gastric environment. J. Appl. Microbiol. 102:352-362. [PubMed]
8. Beuchat, L. R. 1998. Surface decontamination of fruits and vegetables eaten raw: a review. Food Safety Unit, World Health Organization, Geneva, Switzerland.
9. Blattner, F. R., G. Plunkett III, C. A. Bloch, N. T. Perna, V. Burland, M. Riley, J. Collado-Vides, J. D. Glasner, C. K. Rode, G. F. Mayhew, J. Gregor, N. W. Davis, H. A. Kirkpatrick, M. A. Goeden, D. J. Rose, B. Mau, and Y. Shao. 1997. The complete genome sequence of Escherichia coli K-12. Science 277:1453-1474. [PubMed]
10. Brandl, M. T. 2006. Fitness of human enteric pathogens on plants and implications for food safety. Annu. Rev. Phytopathol. 44:367-392. [PubMed]
11. Brouillette, S., and P. Chartrand. 1987. Intermolecular recombination assay for mammalian cells that produces recombinants carrying both homologous and nonhomologous junctions. Mol. Cell. Biol. 7:2248-2255. [PMC free article] [PubMed]
12. Centers for Disease Control and Prevention. 2006. Ongoing multistate outbreak of Escherichia coli serotype O157:H7 infections associated with consumption of fresh spinach—United States, September 2006. MMWR Morb. Mortal. Wkly. Rep. 55:1045-1046. [PubMed]
13. Chesney, J. A., J. W. Eaton, and J. R. Mahoney, Jr. 1996. Bacterial glutathione: a sacrificial defense against chlorine compounds. J. Bacteriol. 178:2131-2135. [PMC free article] [PubMed]
14. Christman, M. F., R. W. Morgan, F. S. Jacobson, and B. N. Ames. 1985. Positive control of a regulon for defenses against oxidative stress and some heat-shock proteins in Salmonella typhimurium. Cell 41:753-762. [PubMed]
15. Clarkson, R. M., A. J. Moule, and H. M. Podlich. 2001. The shelf-life of sodium hypochlorite irrigating solutions. Aust. Dent. J. 46:269-276. [PubMed]
16. Delaquis, S., S. Stewart, S. Cazaux, and P. Toivonen. 2002. Survival and growth of Listeria monocytogenes and Escherichia coli O157:H7 in ready-to-eat iceberg lettuce washed in warm chlorinated water. J. Food Prot. 65:459-464. [PubMed]
17. De Wulf, P., O. Kwon, and E. C. Lin. 1999. The CpxRA signal transduction system of Escherichia coli: growth-related autoactivation and control of unanticipated target operons. J. Bacteriol. 181:6772-6778. [PMC free article] [PubMed]
18. Donnenberg, M. S., A. Donohue-Rolfe, and G. T. Keusch. 1989. Epithelial cell invasion: an overlooked property of enteropathogenic Escherichia coli (EPEC) associated with the EPEC adherence factor. J. Infect. Dis. 160:452-459. [PubMed]
19. Feng, P., K. A. Lampel, H. Karch, and T. S. Whittam. 1998. Genotypic and phenotypic changes in the emergence of Escherichia coli O157:H7. J. Infect. Dis. 177:1750-1753. [PubMed]
20. Gon, S., and J. Beckwith. 2006. Ribonucleotide reductases: influence of environment on synthesis and activity. Antioxid. Redox Signal. 8:773-780. [PubMed]
21. Gonzalez, C. F., M. Proudfoot, G. Brown, Y. Korniyenko, H. Mori, A. V. Savchenko, and A. F. Yakunin. 2006. Molecular basis of formaldehyde detoxification. Characterization of two S-formylglutathione hydrolases from Escherichia coli, FrmB and YeiG. J. Biol. Chem. 281:14514-14522. [PubMed]
22. Gustavsson, N., A. Diez, and T. Nystrom. 2002. The universal stress protein paralogues of Escherichia coli are co-ordinately regulated and co-operate in the defence against DNA damage. Mol. Microbiol. 43:107-117. [PubMed]
23. Harris, L. J., J. N. Farber, L. R. Beuchat, M. E. Parish, and T. V. Suslow. 2003. Outbreaks associated with fresh produce: incidence, growth and survival of pathogens in fresh and fresh-cut produce. Comp. Rev. Food Sci. Food Safety 2:78-141.
24. Hartl, F. U., and M. Hayer-Hartl. 2002. Molecular chaperones in the cytosol: from nascent chain to folded protein. Science 295:1852-1858. [PubMed]
25. Hayashi, T., K. Makino, M. Ohnishi, K. Kurokawa, K. Ishii, K. Yokoyama, C. G. Han, E. Ohtsubo, K. Nakayama, T. Murata, M. Tanaka, T. Tobe, T. Iida, H. Takami, T. Honda, C. Sasakawa, N. Ogasawara, T. Yasunaga, S. Kuhara, T. Shiba, M. Hattori, and H. Shinagawa. 2001. Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12. DNA Res. 8:11-22. [PubMed]
26. Hayes, P. S., K. Blom, P. Feng, J. Lewis, N. A. Strockbine, and B. Swaminathan. 1995. Isolation and characterization of a β-d-glucuronidase-producing strain of Escherichia coli serotype O157:H7 in the United States. J. Clin. Microbiol. 33:3347-3348. [PMC free article] [PubMed]
27. Herbelin, C. J., S. C. Chirillo, K. A. Melnick, and T. S. Whittam. 2000. Gene conservation and loss in the mutS-rpoS genomic region of pathogenic Escherichia coli. J. Bacteriol. 182:5381-5390. [PMC free article] [PubMed]
28. Herring, C. D., and F. R. Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186:6714-6720. [PMC free article] [PubMed]
29. Hirakawa, H., K. Nishino, T. Hirata, and A. Yamaguchi. 2003. Comprehensive studies of drug resistance mediated by overexpression of response regulators of two-component signal transduction systems in Escherichia coli. J. Bacteriol. 185:1851-1856. [PMC free article] [PubMed]
30. Imlay, J. A. 2003. Pathways of oxidative damage. Annu. Rev. Microbiol. 57:395-418. [PubMed]
31. Imlay, K. R., and J. A. Imlay. 1996. Cloning and analysis of sodC, encoding the copper-zinc superoxide dismutase of Escherichia coli. J. Bacteriol. 178:2564-2571. [PMC free article] [PubMed]
32. Kanai, T., K. Takahashi, and H. Inoue. 2006. Three distinct-type glutathione S-transferases from Escherichia coli important for defense against oxidative stress. J. Biochem. 140:703-711. [PubMed]
33. Karmali, M. A., M. Mascarenhas, M. Petric, L. Dutil, K. Rahn, K. Ludwig, G. S. Arbus, P. Michel, P. M. Sherman, J. Wilson, R. Johnson, and J. B. Kaper. 2003. Age-specific frequencies of antibodies to Escherichia coli verocytotoxins (Shiga toxins) 1 and 2 among urban and rural populations in southern Ontario. J. Infect. Dis. 188:1724-1729. [PubMed]
34. Kempf, B., and E. Bremer. 1998. Uptake and synthesis of compatible solutes as microbial stress responses to high-osmolality environments. Arch. Microbiol. 170:319-330. [PubMed]
35. Kim, J., J. Nietfeldt, and A. K. Benson. 1999. Octamer-based genome scanning distinguishes a unique subpopulation of Escherichia coli O157:H7 strains in cattle. Proc. Natl. Acad. Sci. USA 96:13288-13293. [PubMed]
36. Kim, Y., S. Oh, S. Park, and S. H. Kim. 2009. Interactive transcriptome analysis of enterohemorrhagic Escherichia coli (EHEC) O157:H7 and intestinal epithelial HT-29 cells after bacterial attachment. Int. J. Food Microbiol. 131:224-232. [PubMed]
37. Klein, E. J., J. R. Stapp, C. R. Clausen, D. R. Boster, J. G. Wells, X. Qin, D. L. Swerdlow, and P. I. Tarr. 2002. Shiga toxin-producing Escherichia coli in children with diarrhea: a prospective point-of-care study. J. Pediatr. 141:172-177. [PubMed]
38. Kozliak, E. I., M. B. Guilloton, M. Gerami-Nejad, J. A. Fuchs, and P. M. Anderson. 1994. Expression of proteins encoded by the Escherichia coli cyn operon: carbon dioxide-enhanced degradation of carbonic anhydrase. J. Bacteriol. 176:5711-5717. [PMC free article] [PubMed]
39. Kresse, A. U., K. Schulze, C. Deibel, F. Ebel, M. Rohde, T. Chakraborty, and C. A. Guzman. 1998. Pas, a novel protein required for protein secretion and attaching and effacing activities of enterohemorrhagic Escherichia coli. J. Bacteriol. 180:4370-4379. [PMC free article] [PubMed]
40. Krishnan, N., M. B. Dickman, and D. F. Becker. 2008. Proline modulates the intracellular redox environment and protects mammalian cells against oxidative stress. Free Radic. Biol. Med. 44:671-681. [PMC free article] [PubMed]
41. Krishnan, N., A. R. Doster, G. E. Duhamel, and D. F. Becker. 2008. Characterization of a Helicobacter hepaticus putA mutant strain in host colonization and oxidative stress. Infect. Immun. 76:3037-3044. [PMC free article] [PubMed]
42. Lang, M. M., L. J. Harris, and L. R. Beuchat. 2004. Survival and recovery of Escherichia coli O157:H7, Salmonella, and Listeria monocytogenes on lettuce and parsley as affected by method of inoculation, time between inoculation and analysis, and treatment with chlorinated water. J. Food Prot. 67:1092-1103. [PubMed]
43. Lawrence, J. G., and H. Ochman. 1998. Molecular archaeology of the Escherichia coli genome. Proc. Natl. Acad. Sci. USA 95:9413-9417. [PubMed]
44. Leenanon, B., and M. A. Drake. 2001. Acid stress, starvation, and cold stress affect poststress behavior of Escherichia coli O157:H7 and nonpathogenic Escherichia coli. J. Food Prot. 64:970-974. [PubMed]
45. Le Rudulier, D., A. R. Strom, A. M. Dandekar, L. T. Smith, and R. C. Valentine. 1984. Molecular biology of osmoregulation. Science 224:1064-1068. [PubMed]
46. Li, Y., R. E. Brackett, J. Chen, and L. R. Beuchat. 2001. Survival and growth of Escherichia coli O157:H7 inoculated onto cut lettuce before or after heating in chlorinated water, followed by storage at 5 or 15 degrees C. J. Food Prot. 64:305-309. [PubMed]
47. Lindsey, M. L., J. Yoshioka, C. MacGillivray, S. Muangman, J. Gannon, A. Verghese, M. Aikawa, P. Libby, S. M. Krane, and R. T. Lee. 2003. Effect of a cleavage-resistant collagen mutation on left ventricular remodeling. Circ. Res. 93:238-245. [PubMed]
48. Loschi, L., S. J. Brokx, T. L. Hills, G. Zhang, M. G. Bertero, A. L. Lovering, J. H. Weiner, and N. C. Strynadka. 2004. Structural and biochemical identification of a novel bacterial oxidoreductase. J. Biol. Chem. 279:50391-50400. [PubMed]
49. Manning, S. D., A. S. Motiwala, A. C. Springman, W. Qi, D. W. Lacher, L. M. Ouellette, J. M. Mladonicky, P. Somsel, J. T. Rudrik, S. E. Dietrich, W. Zhang, B. Swaminathan, D. Alland, and T. S. Whittam. 2008. Variation in virulence among clades of Escherichia coli O157:H7 associated with disease outbreaks. Proc. Natl. Acad. Sci. USA 105:4868-4873. [PubMed]
50. Marques, L. R., M. A. Moore, J. G. Wells, I. K. Wachsmuth, and A. D. O'Brien. 1986. Production of Shiga-like toxin by Escherichia coli. J. Infect. Dis. 154:338-341. [PubMed]
51. Meyer, C., G. Bierbaum, C. Heidrich, M. Reis, J. Suling, M. I. Iglesias-Wind, C. Kempter, E. Molitor, and H. G. Sahl. 1995. Nucleotide sequence of the lantibiotic Pep5 biosynthetic gene cluster and functional analysis of PepP and PepC. Evidence for a role of PepC in thioether formation. Eur. J. Biochem. 232:478-489. [PubMed]
52. Munn, C. B., H. K. Marchant, and A. J. Moody. 2008. Defences against oxidative stress in vibrios associated with corals. FEMS Microbiol. Lett. 281:58-63. [PubMed]
53. Nordlund, P., and P. Reichard. 2006. Ribonucleotide reductases. Annu. Rev. Biochem. 75:681-706. [PubMed]
54. Park, S., X. You, and J. A. Imlay. 2005. Substantial DNA damage from submicromolar intracellular hydrogen peroxide detected in Hpx- mutants of Escherichia coli. Proc. Natl. Acad. Sci. USA 102:9317-9322. [PubMed]
55. Pattison, D. I., and M. J. Davies. 2001. Absolute rate constants for the reaction of hypochlorous acid with protein side chains and peptide bonds. Chem. Res. Toxicol. 14:1453-1464. [PubMed]
56. Perna, N. T., G. Plunkett III, V. Burland, B. Mau, J. D. Glasner, D. J. Rose, G. F. Mayhew, P. S. Evans, J. Gregor, H. A. Kirkpatrick, G. Posfai, J. Hackett, S. Klink, A. Boutin, Y. Shao, L. Miller, E. J. Grotbeck, N. W. Davis, A. Lim, E. T. Dimalanta, K. D. Potamousis, J. Apodaca, T. S. Anantharaman, J. Lin, G. Yen, D. C. Schwartz, R. A. Welch, and F. R. Blattner. 2001. Genome sequence of enterohaemorrhagic Escherichia coli O157:H7. Nature 409:529-533. [PubMed]
57. Pfaffl, M. W. 2001. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 29:e45. [PMC free article] [PubMed]
58. Pogliano, J., A. S. Lynch, D. Belin, E. C. Lin, and J. Beckwith. 1997. Regulation of Escherichia coli cell envelope proteins involved in protein folding and degradation by the Cpx two-component system. Genes Dev. 11:1169-1182. [PubMed]
59. Pupo, G. M., D. K. Karaolis, R. Lan, and P. R. Reeves. 1997. Evolutionary relationships among pathogenic and nonpathogenic Escherichia coli strains inferred from multilocus enzyme electrophoresis and mdh sequence studies. Infect. Immun. 65:2685-2692. [PMC free article] [PubMed]
60. Raffa, R. G., and T. L. Raivio. 2002. A third envelope stress signal transduction pathway in Escherichia coli. Mol. Microbiol. 45:1599-1611. [PubMed]
61. Rangel, J. M., P. H. Sparling, C. Crowe, P. M. Griffin, and D. L. Swerdlow. 2005. Epidemiology of Escherichia coli O157:H7 outbreaks, United States, 1982-2002. Emerg. Infect. Dis. 11:603-609. [PMC free article] [PubMed]
62. Rice, C. D., J. E. Pollard, Z. T. Lewis, and W. R. McCleary. 2009. Employment of a promoter-swapping technique shows that PhoU modulates the activity of the PstSCAB2 ABC transporter in Escherichia coli. Appl. Environ. Microbiol. 75:573-582. [PMC free article] [PubMed]
63. Rozen, S., and H. J. Skaletsky. 2000. Primer3 on the WWW for general users and for biologist programmers, p. 365-386. In S. Krawetz and S. Misener (ed.), Methods in molecular biology. Humana Press, Totowa, NJ. [PubMed]
64. Rutala, W. A., E. C. Cole, C. A. Thomann, and D. J. Weber. 1998. Stability and bactericidal activity of chlorine solutions. Infect. Control Hosp. Epidemiol. 19:323-327. [PubMed]
65. Ryu, J. H., and L. R. Beuchat. 2005. Biofilm formation by Escherichia coli O157:H7 on stainless steel: effect of exopolysaccharide and curli production on its resistance to chlorine. Appl. Environ. Microbiol. 71:247-254. [PMC free article] [PubMed]
66. Saby, S., P. Leroy, and J. C. Block. 1999. Escherichia coli resistance to chlorine and glutathione synthesis in response to oxygenation and starvation. Appl. Environ. Microbiol. 65:5600-5603. [PMC free article] [PubMed]
67. Saeed, A. I., V. Sharov, J. White, J. Li, W. Liang, N. Bhagabati, J. Braisted, M. Klapa, T. Currier, M. Thiagarajan, A. Sturn, M. Snuffin, A. Rezantsev, D. Popov, A. Ryltsov, E. Kostukovich, I. Borisovsky, Z. Liu, A. Vinsavich, V. Trush, and J. Quackenbush. 2003. TM4: a free, open-source system for microarray data management and analysis. BioTechniques 34:374-378. [PubMed]
68. Salmon, K., S. P. Hung, K. Mekjian, P. Baldi, G. W. Hatfield, and R. P. Gunsalus. 2003. Global gene expression profiling in Escherichia coli K12. The effects of oxygen availability and FNR. J. Biol. Chem. 278:29837-29855. [PubMed]
69. Sapers, G. M., J. R. Gorney, and A. E. Yousef (ed.). 2006. Microbiology of fruits and vegetables. CRC Press, Boca Raton, FL.
70. Schröder, H., T. Langer, F. U. Hartl, and B. Bukau. 1993. DnaK, DnaJ and GrpE form a cellular chaperone machinery capable of repairing heat-induced protein damage. EMBO J. 12:4137-4144. [PubMed]
71. Schwan, W. R., K. J. Wetzel, T. S. Gomez, M. A. Stiles, B. D. Beitlich, and S. Grunwald. 2004. Low-proline environments impair growth, proline transport and in vivo survival of Staphylococcus aureus strain-specific putP mutants. Microbiology 150:1055-1061. [PubMed]
72. Seaver, L. C., and J. A. Imlay. 2001. Alkyl hydroperoxide reductase is the primary scavenger of endogenous hydrogen peroxide in Escherichia coli. J. Bacteriol. 183:7173-7181. [PMC free article] [PubMed]
73. Seo, K. H., and J. F. Frank. 1999. Attachment of Escherichia coli O157:H7 to lettuce leaf surface and bacterial viability in response to chlorine treatment as demonstrated by using confocal scanning laser microscopy. J. Food Prot. 62:3-9. [PubMed]
74. Shaikh, N., and P. I. Tarr. 2003. Escherichia coli O157:H7 Shiga toxin-encoding bacteriophages: integrations, excisions, truncations, and evolutionary implications. J. Bacteriol. 185:3596-3605. [PMC free article] [PubMed]
75. Shijuku, T., T. Yamashino, H. Ohashi, H. Saito, T. Kakegawa, M. Ohta, and H. Kobayashi. 2002. Expression of chaA, a sodium ion extrusion system of Escherichia coli, is regulated by osmolarity and pH. Biochim. Biophys. Acta 1556:142-148. [PubMed]
76. Sirtes, G., T. Waltimo, M. Schaetzle, and M. Zehnder. 2005. The effects of temperature on sodium hypochlorite short-term stability, pulp dissolution capacity, and antimicrobial efficacy. J. Endod. 31:669-671. [PubMed]
77. Sivapalasingam, S., C. R. Friedman, L. Cohen, and R. V. Tauxe. 2004. Fresh produce: a growing cause of outbreaks of foodborne illness in the United States, 1973 through 1997. J. Food Prot. 67:2342-2353. [PubMed]
78. Toledano, M. B., C. Kumar, N. Le Moan, D. Spector, and F. Tacnet. 2007. The system biology of thiol redox system in Escherichia coli and yeast: differential functions in oxidative stress, iron metabolism and DNA synthesis. FEBS Lett. 581:3598-3607. [PubMed]
79. Touati, D. 2000. Sensing and protecting against superoxide stress in Escherichia coli—how many ways are there to trigger soxRS response? Redox Rep. 5:287-293. [PubMed]
80. Wick, L. M., W. Qi, D. W. Lacher, and T. S. Whittam. 2005. Evolution of genomic content in the stepwise emergence of Escherichia coli O157:H7. J. Bacteriol. 187:1783-1791. [PMC free article] [PubMed]
81. Winter, J., M. Ilbert, P. C. Graf, D. Ozcelik, and U. Jakob. 2008. Bleach activates a redox-regulated chaperone by oxidative protein unfolding. Cell 135:691-701. [PMC free article] [PubMed]
82. Winter, J., K. Linke, A. Jatzek, and U. Jakob. 2005. Severe oxidative stress causes inactivation of DnaK and activation of the redox-regulated chaperone Hsp33. Mol. Cell 17:381-392. [PubMed]
83. Zhang, W., W. Qi, T. J. Albert, A. S. Motiwala, D. Alland, E. K. Hyytia-Trees, E. M. Ribot, P. I. Fields, T. S. Whittam, and B. Swaminathan. 2006. Probing genomic diversity and evolution of Escherichia coli O157 by single nucleotide polymorphisms. Genome Res. 16:757-767. [PubMed]
84. Zhang, X. S., R. Garcia-Contreras, and T. K. Wood. 2007. YcfR (BhsA) influences Escherichia coli biofilm formation through stress response and surface hydrophobicity. J. Bacteriol. 189:3051-3062. [PMC free article] [PubMed]
85. Zheng, M., X. Wang, L. J. Templeton, D. R. Smulski, R. A. LaRossa, and G. Storz. 2001. DNA microarray-mediated transcriptional profiling of the Escherichia coli response to hydrogen peroxide. J. Bacteriol. 183:4562-4570. [PMC free article] [PubMed]
86. Zook, C. D., F. F. Busta, and L. J. Brady. 2001. Sublethal sanitizer stress and adaptive response of Escherichia coli O157:H7. J. Food Prot. 64:767-769. [PubMed]

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