PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of diacareAmerican Diabetes AssociationSubscribeSearchDiabetes Care Journal
 
Diabetes Care. 2009 October; 32(10): 1904–1906.
Published online 2009 July 10. doi:  10.2337/dc09-0663
PMCID: PMC2752934

Maternal BMI Before Pregnancy, Maternal Weight Gain During Pregnancy, and Risk of Persistent Positivity for Multiple Diabetes-Associated Autoantibodies in Children With the High-Risk HLA Genotype

The MIDIA study

Abstract

OBJECTIVE

To assess whether maternal BMI before pregnancy and weight gain during pregnancy predicted the risk of islet autoimmunity in genetically susceptible children.

RESEARCH DESIGN AND METHODS

Of 46,939 newborns screened for the high-risk HLA genotype DR4-DQ8/DR3-DQ2, 1,003 were positive and 885 were followed with serial blood samples tested for autoantibodies to insulin, GAD, and insulinoma-associated protein 2 (IA2). The end point was defined as repeated positivity for two or three autoantibodies or the onset of type 1 diabetes (islet autoimmunity).

RESULTS

Thirty-six children developed islet autoimmunity, of whom 10 developed type 1 diabetes. Both maternal BMI ≥30 kg/m2 before pregnancy and maternal weight gain ≥15 kg predicted the increased risk of islet autoimmunity (hazard ratio [HR] 2.5, P = 0.023, and HR 2.5, P = 0.015, respectively), independent of maternal diabetes.

CONCLUSIONS

Maternal weight may predict risk of islet autoimmunity in offspring with a high genetic susceptibility for type 1 diabetes.

Type 1 diabetes is caused by specific autoimmunity against pancreatic β-cells. The incidence of type 1 diabetes is increasing worldwide, and Norway currently has one of the world's highest incidence rates (1,2). The etiology is multifactorial, determined by a combination of genetic and nongenetic factors. In Norway, 2.1% of newborns carry the HLA genotype DR4-DQ8/DR3-DQ2, which confers a relative risk for type 1 diabetes in excess of 20 and an estimated absolute risk of 7% by age 15 years (3,4). Nongenetic factors have been difficult to identify. Islet autoimmunity may start as early as in the 1st year of life before clinical type 1 diabetes with variable duration or even in utero (5). Studies have suggested that growth and obesity in childhood are associated with risk of type 1 diabetes and islet autoimmunity (6,7), but we are not aware of previous studies investigating the role of maternal BMI or weight gain in pregnancy.

RESEARCH DESIGN AND METHODS

From July 2001 to December 2007, the MIDIA (Norwegian acronym for “Environmental Triggers of Type 1 Diabetes”) study recruited newborns from the general population of Norway with an HLA genotype DR4-DQ8/DR3-DQ2 (DRB1*0401-DQA1*03-DQB1*0302/DRB1*03-DQA1*05-DQB1*02) conferring high risk of type 1 diabetes. Of the 46,939 newborns genotyped, 1,003 (2.14%) carried the high-risk genotype. From that group, 885 children were followed longitudinally with questionnaires and gave blood samples for autoantibody testing at age 3, 6, 9, and 12 months and then annually (8). Children who tested positive for autoantibodies at the age of 12 months or older were scheduled for retesting more frequently than every 12 months. If positive for a single autoantibody, the children were scheduled for retesting every 6th month; if positive for two or three autoantibodies, they were scheduled for retesting every 3rd month. Autoantibodies to insulin, GAD, and insulinoma-associated protein 2 (IA2) were measured using radiobinding assays at the Hormone Laboratory at Aker University Hospital (8), which has participated in the Antibody Standardization Program (DASP) since 2003 (9). In 2007, the disease specificity was 91% for anti-GAD, 95% for anti-IA2, and 96% for insulin autoantibody (IAA), while the disease sensitivity was 50% for anti-GAD, 64% for anti-IA2, and 22% for IAA. Positivity for two or more islet autoantibodies is a strong predictor for type 1 diabetes in young children (10). The end point in this analysis was defined as repeated positivity for two or more of the above-mentioned islet autoantibodies (on at least two consecutive occasions) or the onset of type 1 diabetes (islet autoimmunity). All cases of islet autoimmunity were negative for autoantibodies at age 3 months, and islet autoantibodies potentially originating from the mother were excluded from the end point definition (8). Mother's weight, height, and other demographic data (Table 1) were collected using mailed structured questionnaires when each child was 3 months old, with follow-up information at age 6, 9, and 12 months. Cox proportional hazards regression models were used to estimate hazard ratios (HRs) and 95% CIs for islet autoimmunity using Stata (version 10; College Station, TX). Follow-up time was counted from birth to the date of the last blood sample (noncases) or to the midpoint between the date of the last negative blood sample for islet autoantibodies and the date of the first positive sample for islet autoantibodies (cases with islet autoimmunity).

Table 1
Association of maternal BMI before pregnancy, weight gain during pregnancy, and other characteristics with risk of persistent positivity for multiple islet autoantibodies in children in the MIDIA study

RESULTS

The descriptive characteristics are shown in Table 1. Both maternal BMI ≥30 kg/m2 before pregnancy (relative to <25 kg/m2) and weight gain of ≥15 kg during pregnancy (relative to <15 kg) predicted an approximate two- to threefold increase in the risk of islet autoimmunity shown in Table 1. The estimated HRs were only marginally influenced by mutual adjustment or by adjustment for other potential confounding factors listed in Table 1. For instance, in a regression model simultaneously including maternal BMI ≥30 kg/m2 before pregnancy, maternal weight gain ≥15 kg during pregnancy, presence of first-degree relatives with type 1 diabetes, child's weight gain at 3–12 months of age, duration of total breastfeeding, duration of exclusive breastfeeding, birth order, and smoking in pregnancy, the HR (95% CI) for BMI ≥30 kg/m2 was 2.27 (1.004–5.15) and for maternal weight gain ≥15 kg was 2.60 (1.25–5.41).

CONCLUSIONS

Maternal obesity before pregnancy and weight gain (≥15 kg) during pregnancy significantly predicted increased risk of persistent multiple positivity for islet autoantibodies in offspring with high genetic susceptibility for type 1 diabetes.

Many factors are associated with women's BMI and pregnancy weight gain, such as diet, physical activity, and socioeconomic status (11), but few such factors are known to influence their children's risk of developing islet autoimmunity or type 1 diabetes (12), making confounding by such factors less likely. Maternal diabetes is among the most obvious potential confounders, but controlling for this in the regression analyses had little influence on our main result. Consistent results after adjustment for the child's weight gain, age at weaning, or duration of exclusive breastfeeding, and also after restricting the analyses to cases seroconverting before 12 months of age (data not shown), suggests that a potential role of postnatal factors is less likely to explain our observations. A potential weakness in our study is that maternal height and weight were self-reported. In the event that self-reporting leads to some measurement error, the fact that height and weight were reported before the mothers had any information of islet autoantibody positivity makes it most likely that any bias would have been nondifferential with respect to the end point in our study and thus attenuated the observed relation with islet autoimmunity. Unfortunately, we did not have any measure of insulin sensitivity of the mothers, but this could perhaps be included in future studies.

In conclusion, the current study adds to the evidence that factors operating early in life may influence the risk of advanced islet autoimmunity, which in itself strongly predicts type 1 diabetes. As with any novel result, independent replication will be needed and further research is warranted to unravel the potential mechanisms.

Acknowledgments

This study was funded by the Research Council of Norway (grants 135893/330, 155300/320, 156477/730, and 166515/V50), the Norwegian Diabetes Association, the Children With Diabetes Foundation (Denver, CO), and NewGeneris (grant Food-CT-2005-016320).

No potential conflicts of interest relevant to this article were reported.

We thank the public health care nurses for their efforts in the recruitment to the MIDIA Study and their follow-up with high-risk children, as well as the staff at the Biobank, Norwegian Institute of Public Health for DNA extraction and genotyping. In particular, we thank all the parents for their efforts in handling their child's type 1 diabetes risk, providing their childrens' blood and fecal samples, and completing questionnaires.

Footnotes

The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

References

1. DIAMOND Project Group. Incidence and trends of childhood type 1 diabetes worldwide 1990–1999. Diabet Med 2006;23:857–866 [PubMed]
2. Aamodt G, Stene LC, Njølstad PR, Søvik O, Joner G.: The Norwegian Childhood Diabetes Study Group. Spatiotemporal trends and age-period-cohort modeling of the incidence of type 1 diabetes among children aged <15 years in Norway 1973–1982 and 1989–2003. Diabetes Care 2007;30:884–889 [PubMed]
3. Rønningen KS, Spurkland A, Iwe T, Vartdal F, Thorsby E.: Distribution of HLA-DRB1, -DQA1 and -DQB1 alleles and DQA1-DQB1 genotypes among Norwegian patients with insulin-dependent diabetes mellitus. Tissue Antigens 1991;37:105–111 [PubMed]
4. Undlien DE, Friede T, Rammensee HG, Joner G, Dahl-Jørgensen K, Søvik O, Akselsen HE, Knutsen I, Rønningen KS, Thorsby E.: HLA-encoded genetic predisposition in IDDM: DR4 subtypes may be associated with different degrees of protection. Diabetes 1997;46:143–149 [PubMed]
5. Dahlquist GG.: Viruses and other perinatal exposures as initiating events for beta-cell destruction. Ann Med 1997;29:413–417 [PubMed]
6. Couper JJ, Beresford S, Hirte C, Baghurst PA, Pollard A, Tait BD, Harrison LC, Colman PG.: Weight gain in early life predicts risk of islet autoimmunity in children with a first-degree relative with type 1 diabetes. Diabetes Care 2009;32:94–99 [PMC free article] [PubMed]
7. Viner RM, Hindmarsh PC, Taylor B, Cole TJ.: Childhood body mass index (BMI), breastfeeding and risk of type 1 diabetes: findings from a longitudinal national birth cohort. Diabet Med 2008;25:1056–1061 [PubMed]
8. Stene LC, Witsø E, Torjesen PA, Rasmussen T, Magnus P, Cinek O, Wetlesen T, Rønningen KS.: Islet autoantibody development during follow-up of high-risk children from the general Norwegian population from three months of age: design and early results from the MIDIA study. J Autoimmun 2007;29:44–51 [PubMed]
9. Bingley PJ, Bonifacio E, Mueller PW.: participating laboratories. Diabetes Antibody Standardization Program: first assay proficiency evaluation. Diabetes 2003;52:1128–1136 [PubMed]
10. Achenbach P, Warncke K, Reiter J, Naserke HE, Williams AJK, Bingley PJ, Bonifacio E, Ziegler AG.: Stratification of type 1 diabetes risk on the basis of islet autoantibody characteristics. Diabetes 2004;53:384–392 [PubMed]
11. Baker JL, Michaelsen KF, Sørensen TI, Rasmussen KM.: High prepregnant body mass index is associated with early termination of full and any breastfeeding in Danish women. Am J Clin Nutr 2007;86:404–411 [PubMed]
12. Stene LC, Tuomilehto J, Rewers M.: Global epidemiology of type 1 diabetes. In The Epidemiology of Diabetes Mellitus 2nd ed.Ekoé J-M, Rewers M, Williams R, Zimmet P, editors. Eds. Chichester, U.K., John Wiley & Sons, Ltd., 2008, p. 355–383

Articles from Diabetes Care are provided here courtesy of American Diabetes Association