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AAPS PharmSci. Dec 2003; 5(4): 101–111.
Published online Dec 4, 2003. doi:  10.1208/ps050433
PMCID: PMC2750995
A photo-crosslinked poly(vinyl alcohol) hydrogel growth factor release vehicle for wound healing applications
Sharon L. Bourke,corresponding author1 Mohammad Al-Khalili,2 Tonye Briggs,1 Bozena B. Michniak,2 Joachim Kohn,1 and Laura A. Poole-Warrencorresponding author1,3
1New Jersey Center for Biomaterials, Department of Chemistry and Chemical Biology, Rutgers University, 610 Taylor Road, Piscataway, NJ
2New Jersey Center for Biomaterials, Drug Delivery Laboratory, CHEN Building, 111 Lock Street, Newark, NJ
3Graduate School of Biomedical Engineering, University of New South Wales, 2052 Sydney, NSW Australia
Laura A. Poole-Warren, Phone: +61 (2) 9385 3905, Fax: +61 (2) 9663 2108, l.poolewarren/at/unsw.edu.au.
corresponding authorCorresponding author.
Received June 10, 2003; Accepted October 9, 2003.
The objective of this study was to develop and evaluate a hydrogel vehicle for sustained release of growth factors for wound healing applications. Hydrogels were fabricated using ultraviolet photo-crosslinking of acrylamide-functionalized nondegradable poly(vinyl alcohol) (PVA). Protein permeability was initially assessed using trypsin inhibitor (TI), a 21 000 MW model protein drug. TI permeability was altered by changing the solids content of the gel and by adding hydrophilic PVA fillers. As the PVA content increased from 10% to 20%, protein flux decreased, with no TI permeating through 20% PVA hydrogels. Further increase in model drug release was achieved by incorporating hydrophilic PVA fillers into the hydrogel. As filler molecular weight increased, TI flux increased. The mechanism for this is most likely an alteration in protein/gel interactions and transient variations in water content. The percent protein released was also altered by varying protein loading concentration. Release studies conducted using growth factor in vehicles with hydrophilic filler showed sustained release of platelet-derived growth factor (PDGF-β,β) for up to 3 days compared with less than 24 hours in the controls. In vitro bioactivity was demonstrated by doubling of normal human dermal fibroblas numbers when exposed to growth factor-loaded vehicle compared to control. The release vehicle developed in this study uses a rapid and simple fabrication method, and protein release can be tailored by modifying solid content, incorporating biocompatible hydrophilic fillers, and varying protein loading concentration.
Keywords: photo-crosslinkable hydrogel, poly(vinyl alcohol), platelet-derived growth factor, bioactivity, sustained release
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