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AAPS PharmSciTech. 2006 March; 7(1): E215–E220.
Published online 2006 March 24. doi:  10.1208/pt070130
PMCID: PMC2750737

Development and in vitro evaluation of chitosan-Eudragit RS 30D composite wound dressings


The purpose of this research was to design and evaluate chitosan-based films intended for wound dressing application. Required properties for successful wound dressing, such as liquid uptake, vapor and oxygen penetration, bioadhesiveness, and film elasticity, were examined. Water uptake and vapor penetration of the films were determined gravimetrically, while oxygen penetration was determined by Winkler’s method. The bioadhesive properties were determined with an in-house pulley system instrument using a pig gut model. Film elasticity was determined with a stretch test using an Instron apparatus. The results showed that pure chitosan films exhibited relatively high liquid uptake and the adsorption tended to decrease with the addition of Eudragit RS 30D. Moisture vapor and oxygen were found to be able to penetrate through all film formulations in comparable amounts. The bioadhesiveness test tended to show lower bioadhesive properties with the addition of Eudragit RS 30D. The formulation containing only chitosan exhibited low elongation of the film at 2 N, but the film elasticity increased with the addition of Eudragit RS 30D. In conclusion, the addition of Eudragit RS 30D could improve a film’s mechanical properties but lower its bioadhesiveness.

Keywords: wound dressing, chitosan hydrogels, Eudragit RS 30D

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Selected References

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1. Svedman P. Irrigation treatment of legs ulcers. Lancet. 1983;322:532–534. doi: 10.1016/S0140-6736(83)90567-6. [PubMed] [Cross Ref]
2. Lim JK, Saliba L, Smith MJ, MacTavish J, Raine C, Curtin P. Normal saline wound dressing—is it really normal? Br J Plast Surg. 2000;53:42–45. doi: 10.1054/bjps.1999.3246. [PubMed] [Cross Ref]
3. Biagini G, Bertani A, Mazzarelli R, et al. Wound management with N-carboxybutyl chitosan. Biomaterials. 1991;12:281–285. doi: 10.1016/0142-9612(91)90035-9. [PubMed] [Cross Ref]
4. Matsuda K, Suzuki S, Isshiki N, Ikada Y. Re-freeze dried bilayer artificial skin. Biomaterials. 1993;14:1030–1035. doi: 10.1016/0142-9612(93)90197-A. [PubMed] [Cross Ref]
5. Su CH, Sun CS, Juan SW, Hu CH, Ke WT, Sheu MT. Fungal mycelia as the source of chitin and polysaccharides and their applications as skin substitutes. Biomaterials. 1997;18:1169–1174. doi: 10.1016/S0142-9612(97)00048-3. [PubMed] [Cross Ref]
6. Mi F., Shyu S, Wu Y, Lee S, Shyong J, Huang R. Fabrication and characterization of a sponge-like asymmetric chitosan membrane as a wound dressing. Biomaterials. 2001;22:165–173. doi: 10.1016/S0142-9612(00)00167-8. [PubMed] [Cross Ref]
7. Tomihata K, Ikada Y. In vitro and in vivo degradation of films of chitin and its deacetylated derivatives. Biomaterials. 1997;18:567–573. doi: 10.1016/S0142-9612(96)00167-6. [PubMed] [Cross Ref]
8. Abhay SP, inventor. Hemostatic wound dressing. US patent 5 836 970. November 17, 1998.
9. Ueno H, Murakami M, Okumura M, Kadosawa T, Uede T, Fujinaga T. Chitosan accelerates the production of osteopontin from polymorphonuclear leukocytes. Biomaterials. 2001;22:1667–1673. doi: 10.1016/S0142-9612(00)00328-8. [PubMed] [Cross Ref]
10. Suzuki Y, Okamoto Y, Morimoto M, et al. Influence of physicochemical properties of chitin and chitosan on compliment activation. Carbohydr Polym. 2000;42:307–310. doi: 10.1016/S0144-8617(99)00161-7. [Cross Ref]
11. Harris MR, Ghebre-Sellassie I. Aqueous polymeric coating for modified release oral dosage forms. In: McGinity JW, editor. Aqueous Polymeric Coatings for Pharmaceutical Dosage Forms. 2nd ed. New York, NY: Marcel Dekker; 1997. pp. 81–100.
12. Lehman K. Practical Course in Film Coating of Pharmaceutical Dosage Forms with Eudragit. Darmstadt, Germany. Röhm Pharma Polymers. 2001;8–10:144–147.
13. Bodmeier R, Paeratakul O. Mechanical properties of dry and wet cellulosic and acrylic polymer films prepared from aqueous colloidal polymer dispersions. Pharm Res. 1994;11:882–888. doi: 10.1023/A:1018942127524. [PubMed] [Cross Ref]
14. Bodmeier R, Paeratakul O. Process and formation variables affecting the drug release from chlorpheniramine maleate-loaded beads coated with commercial and self-prepared aqueous ethyl cellulose pseudolatexes. Int J Pharm. 1991;70:59–68. doi: 10.1016/0378-5173(91)90164-J. [Cross Ref]
15. Wesseling M, Kuppler F, Bodmeier R. Tackiness of acrylic and cellulosic polymer films used in the coating of solid dosage forms. Eur J Pharm Biopharm. 1999;47:73–78. doi: 10.1016/S0939-6411(98)00091-5. [PubMed] [Cross Ref]
16. Glazer BT, Marsh AG, Stierhoff K, Luther GW. The dynamic response of optical oxygen sensors and voltammetric electrodes to temporal changes in dissolved oxygen concentrations. Anal Chim Acta. 2004;518:93–100. doi: 10.1016/j.aca.2004.05.040. [Cross Ref]
17. Nakatsuka S, Andrady AL. Permeability of vitamin B-12 chitosan membranes: effect of croslinking and blending with poly(vinyl alcohol) on permeability. J Appl Polym Sci. 1992;44:17–28. doi: 10.1002/app.1992.070440103. [Cross Ref]
18. Thacharodi D, Rao KP. Propanolol hydrochloride release behavior of crosslinked chitosan membranes. J Chem Technol Biotechnol. 1993;58:177–181. doi: 10.1002/jctb.280580211. [PubMed] [Cross Ref]
19. Devallencourt C, Marais S, Saiter JM, Labbe M, Metayer M. Study of transport of small molecules through ethyele-co-vinyl acetate copolymers films. Part A: water molecules. Polym Test. 2002;21:253–262. doi: 10.1016/S0142-9418(01)00078-2. [Cross Ref]
20. Okawa Y, Tokoro A, Kobyashi M, Suzuki K, Suzuki S, Suzuki M. Protective effect of chitin, chitosan and their oligosaccharides against bacterial infection in mice. Int J Immunol. 1998;10:122–122. doi: 10.1016/0192-0561(88)90464-X. [Cross Ref]

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