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AAPS PharmSciTech. 2006 March; 7(1): E104–E110.
Published online 2006 February 10. doi:  10.1208/pt070115
PMCID: PMC2750722

The effect of microwave thermal denaturation on release properties of bovine serum albumin and gluten matrices


The purpose of this study was to compare the effects of denaturation by microwave irradiation on release properties of 2 physically different proteins. Matrices were prepared from water-soluble bovine serum albumin loaded with metoclopramide and sorbed with adequate amount of moisture were thermally denatured in a microwave oven. The release profile of the rather insoluble denatured albumin matrices followed the classical Fickian diffusion profile. The release rate was dependent on the degree of denaturation, which was highly dependent on the level of moisture originally absorbed by the albuminoidal matrices and the period of exposure to microwave energy. Consersely, attempts to reduce the rate of drug release through microwave irradiation of metoclopramide-loaded matrices prepared from water-insoluble gluten were futile. The denaturation process was shown to be limited to the relatively water-soluble protein core fraction, while aggregation between neighboring gluten proteins in the matrix was not achieved even in the presence of considerable amounts of sorbed water.

Keywords: microwave, denaturation, gluten, bovine serum albumin, controlled-release

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
1. Micard V, Belamri R, Morel M, Guilbert S. Properties of chemically and physically treated wheat gluten films. J Agric Food Chem. 2000;48:2948–2953. doi: 10.1021/jf0001785. [PubMed] [Cross Ref]
2. Joshi HN, Kral MA, Topp EM. Microwave drying of aqueous tablet film coatings: a study on free films. Int J Pharm. 1989;51:19–25. doi: 10.1016/0378-5173(89)90070-7. [Cross Ref]
3. Mandal TK. Evaluation of microwave drying for pharmaceutical granulations. Drug Dev Ind Pharm. 1995;21:1683–1688. doi: 10.3109/03639049509069257. [Cross Ref]
4. McLoughlin CM, McMinn WAM, Magee TRA. Microwave drying of pharmaceutical powders. Food Bioprod Process. 2000;78:90–96. doi: 10.1205/096030800532798. [Cross Ref]
5. Vromans H. Microwave drying of pharmaceutical excipients: comparison with conventional conductive drying. Eur J Pharm Biopharm. 1994;40:333–336.
6. Larhed M, Hallberg A. Microwave-assisted high speed chemistry: a new technique in drug discovery. Drug Discov Today. 2001;6:406–416. doi: 10.1016/S1359-6446(01)01735-4. [PubMed] [Cross Ref]
7. Lidström P, Tierney J, Wathey B, Westman J. Microwave assisted organic synthesis: a review. Tetrahedron. 2001;57:9225–9283. doi: 10.1016/S0040-4020(01)00906-1. [Cross Ref]
8. Beary ES. Comparison of microwave drying and conventional drying techniques for reference materials. Anal Chem. 1988;60:742–746. doi: 10.1021/ac00159a003. [Cross Ref]
9. Teng CD, Groves MJ. Microwave thermal denaturation of protein matrices as controlled release devives. J Control Release. 1990;13:43–49. doi: 10.1016/0168-3659(90)90073-3. [Cross Ref]
10. Vandelli MA, Romagnoli M, Monti A, et al. Microwave-treated gelatin microspheres as drug delivery system. J Control Release. 2004;96:67–84. doi: 10.1016/j.jconrel.2004.01.009. [PubMed] [Cross Ref]
11. Wong TW, Chan LW, Kho SB, Heng PWS. Design of controlled-release solid dosage forms of alginate and chitosan using microwave. J Control Release. 2002;84:99–114. doi: 10.1016/S0168-3659(02)00237-7. [PubMed] [Cross Ref]
12. Yoshida S, Yasuda K, Kuriyama M, Kanayama H, inventors. Manufacture of (Co)polymers of Lactic Acid and or Glycolic acid. Japan Patent 63 289 020. November 1988.
13. Lu J, Zhu X, Ji S, Zhu J, Chen Z. Microwave radiation copolymerization in solid state of maleic anhydride and allythiourea. J Appl Polym Sci. 1998;68:1563–1566. doi: 10.1002/(SICI)1097-4628(19980606)68:10<1563::AID-APP3>3.0.CO;2-F. [Cross Ref]
14. Teng CD, Alkan MH, Groves MJ. Effect of adsorbed water on compaction properties and the dissolution of quinacrine hydrochloride from compacted matrices of soy protein. Drug Dev Ind Pharm. 1986;12:2325–2336. doi: 10.3109/03639048609042639. [Cross Ref]
15. Yeargers EK, Langley JB, Sheppard AP, Huddleston GK. Effects of microwave radiation on enzymes. Ann N Y Acad Sci. 1975;247:301–304. doi: 10.1111/j.1749-6632.1975.tb36005.x. [PubMed] [Cross Ref]
16. Peppas NA. Analysis of Fickian and non-Fickian drug release from polymers. Pharm Acta Helv. 1985;60:110–111. [PubMed]
17. Gennadios A, Weller CL. Edible films and coatings from wheat and corn proteins. Food Technol. 1990;44:63–69.
18. Teikoku Chemical Industry Co, Ltd., inventor. Transdermal Tapes Containing Nifidipine and Glutens. Japan Patent 59196814. November 1984.

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