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AAPS PharmSciTech. 2007 October; 8(4): 43–49.
Published online 2007 October 19. doi:  10.1208/pt0804086
PMCID: PMC2750672

Formulation and optimization of piroxicam proniosomes by 3-factor, 3-level box-behnken design

Abstract

The aim of this study was to investigate the combined influence of 3 independent variables in the preparation of piroxicam proniosomes by the slurry method. A 3-factor, 3-level Box-Behnken design was used to derive a second-order polynomial equation and construct contour plots to predict responses. The independent variables selected were molar ratio of Span 60:cholesterol (X1), surfactant loading (X2), and amount of drug (X3). Fifteen batches were prepared by the slurry method and evaluated for percentage drug entrapment (PDE) and vesicle size. The transformed values of the independent variables and the PDE (dependent variable) were subjected to multiple regression to establish a full-model second-order polynomial equation. F was calculated to confirm the omission of insignificant terms from the full-model equation to derive a reduced-model polynomial equation to predict the PDE of proniosome-derived niosomes. Contour plots were constructed to show the effects of X1, X2 and X3 on the PDE. A model was validated for accurate prediction of the PDE by performing checkpoint analysis. The computer optimization process and contour plots predicted the levels of independent variables X1, X2, and X3 (0, −0.158 and -0.158 respectively), for maximized response of PDE with constraints on vesicle size. The Box-Behnken design demonstrated the role of the derived equation and contour plots in predicting the values of dependent variables for the preparation and optimization of piroxicam proniosomes.

Keywords: Proniosomes, niosomes, Box-Behnken design, optimization

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
1. Biju SS, Talegaonkar S, Misra PR, Khar RK. Vesicular systems: an overview. Indian J Pharm Sci. 2006;68:141–153.
2. Azmin MN, Florence AT, Handjani-Vila RM, Stuart JFB, Vanlerberghe G, Whittaker JS. The effect of non-ionic surfactant vesicle (niosome) entrapment on the absorption and distribution of methotrexate in mice. J Pharm Pharmacol. 1985;37:237–243. [PubMed]
3. Baillie AJ, Florence AT, Hume LR, Muirhead GT, Rogerson A. The preparation and properties of niosomes—non-ionic surfactant vesicles. J Pharm Pharmacol. 1985;37:863–868. [PubMed]
4. Namdeo A, Jain NK. Niosomal delivery of 5-fluorouracil. J Microencapsul. 1999;16:731–740. doi: 10.1080/026520499288672. [PubMed] [Cross Ref]
5. Hu C, Rhodes DG. Proniosomes: a novel drug carrier preparation. Int J Pharm. 1999;185:23–35. doi: 10.1016/S0378-5173(99)00122-2. [PubMed] [Cross Ref]
6. Blazek-Welsh AI, Rhodes DG. Maltodextrin-based proniosomes.AAPS PharmSci [serial online]. 2001;3:article 1.
7. Blazek-Welsh AI, Rhodes DG. SEM imaging predicts quality of niosomes from maltodextrin-based proniosomes. Pharm Res. 2001;18:656–661. doi: 10.1023/A:1011037527889. [PubMed] [Cross Ref]
8. Gohel MC, Amin AF. Formulation optimization of controlled release diclofenac sodium microspheres using factorial design. J Control Release. 1998;51:115–122. doi: 10.1016/S0168-3659(97)00102-8. [PubMed] [Cross Ref]
9. Nazzal S, Khan MA. Response surface methodology for the optimization of ubiquinone self-nanoemulsified drug delivery system.AAPS PharmSciTech [serial online]. 2002;3:article 3. [PMC free article] [PubMed]
10. Li S, Lin S, Chien YW, Daggy BP, Mirchandani HL. Statistical optimization of gastric floating system for oral controlled delivery of calcium.AAPS PharmSciTech [serial online]. 2001;2:article 1. [PMC free article] [PubMed]
11. Box GEP, Behnken DW. Some new three level designs for the study of quantitative variables. Technometrics. 1960;2:455–475. doi: 10.2307/1266454. [Cross Ref]
12. New RRC. Liposomes: A Practical Approach. New York, NY: Oxford University Press; 1990. pp. 80–80.

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