PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of aapspharmspringer.comThis journalToc AlertsSubmit OnlineOpen Choice
 
AAPS PharmSciTech. 2003 December; 4(4): 449–454.
Published online 2003 September 2. doi:  10.1208/pt040457
PMCID: PMC2750650

Investigations on neomycin production with immobilized cells ofStreptomyces marinensis Nuv-5 in calcium alginate matrix

Abstract

The purpose of this investigation was to study the effect ofStreptomyces marinensis NUV-5 cells immobilized in calcium alginate for the production of neomycin. The effect of various parameters, such as the effect of alginate concentration (1%, 2%, 3%, 4%, and 5% wt/vol), the effect of cation (caCl2, BaCl2, and SrCl2), the concentration of cation (0.01M, 0.125M, 0.25M, 0.375M, and 0.5M), the curing times (1, 6, 11, 16, and 21 hours), and the diameter of the bead (1.48, 2.16, 3.24, 4.46, and 5.44 mm), on neomycin production and bead stability were studied. The effect of maltose (4%, 3%, 2%, and 1% wt/vol) and sodium glutamate (0.6%, 0.3%, 0.15%, and 0.075%) wt/vol) concentration on neomycin production was also studied. Better neomycin production was achieved with optimized parameters, such as alginate at 2% wt/vol, 0.25M CaCl2, 1-hour curing time, and 3.24 mm bead diameter. Effective neomycin production was achieved with 3% wt/vol maltose and 0.6% wt/vol sodium glutamate concentration. The repeated batch fermentations were conducted (every 96 hours) using the optimized alginate beads, employing the production medium with 3% wt/vol maltose and 0.6% wt/vol sodium glutamate along with minerals salts solution. The increase in antibiotic production was observed up to the 5th cycle, and later gradual decrease in antibiotic production was observed. Comparison of the total antibiotic production with free cells and immobilized cells was also done. An enhanced antibiotic productivity of 32% was achieved with immobilized cells over the conventional free-cell fermentation, while 108% more productivity was achieved over the washed free-cell fermentation. From these results it is concluded that the immobilized cells ofS marinensis NUV-5 in calcium alginate are more efficient for the production of neomycin with repeated batch fermentation.

Keywords: neomycin production, Streptomyces marinensis NUV-5, immobilized cells

Full Text

The Full Text of this article is available as a PDF (260K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
1. Waksman SA, Lechvalier HA. Neomycin, a new antibiotic active against strep tomycin resistant bacteria, including tuberculosis organisms. Science. 1949;19:305–305. doi: 10.1126/science.109.2830.305. [PubMed] [Cross Ref]
2. Sambamurthy K, Ellaiah P. A new Streptomycete producing neomycin (B&C) complex—S. marinensis (part I) Hindustan Antibiot Bullet. 1974;17:24–27. [PubMed]
3. Lechevalier HA. The 25 years of neomycin. CRC Crit Rev Microbiol. 1975;3(4):359–397. doi: 10.3109/10408417509108756. [PubMed] [Cross Ref]
4. Dulmage HT. The production of neomycin byStreptomyces fradiae in synthetic media. Appl Microbiol. 1953;1:103–106. [PMC free article] [PubMed]
5. Sebek OK. Synthesis of neomycin C14 byStreptomyces fradiae. Arch Biochem Biophys. 1995;57:71–79. doi: 10.1016/0003-9861(55)90178-8. [PubMed] [Cross Ref]
6. Majumdar MK, Majumdar SK. Utilization of carbon and nitrogen containing compounds for neomycin production byStreptomyces fradiae. Appl Microbiol. 1967;15:744–749. [PMC free article] [PubMed]
7. Mangallam S, Vashi AA, Sukapure RS, Gopalakrishnan KS. Some factors affecting neomycin production byS. fradiae. Hindustan Antibiot Bull. 1974;17:1–6. [PubMed]
8. Elliah P, Srinivasulu B, Adinarayana K. Optimization studies on neomycin production by a mutant strain ofStreptomyces marinensis in solid state fermentation.Process Biochem. In press.
9. Adinarayana K, Ellaiah P, Srinivasulu B, Bhavani Devi R, Adinarayana G. Response surface methodological approach to optimize the nutritional parameters for neomycin production byStreptomyces marinensis under solid state fermentation. Process Biochem. 2003;38:1565–1572. doi: 10.1016/S0032-9592(03)00057-8. [Cross Ref]
10. Srinivasulu B. Studies on Bioprocess Development With Special Reference to Strain Improvement, Solid State Fermentation and Immobilization ofStreptomyces marinensis Cells for the Production of Neomycin. Visakhapatnam, India: Andhra University; 2002.
11. Park YS, Ohata N, Okabe M. Neomycin production by partial immobilization ofStreptomyces fradiae on cellulose beads in airlift reactor. J Ferment Bioengg. 1994;78:265–268. doi: 10.1016/0922-338X(94)90303-4. [Cross Ref]
12. Kierstan M, Bucke C. The immobilization of microbial cells, subcellular organelles, and enzymes in calcium alginate gels. Biotechnol Bioeng. 1977;19:387–397. doi: 10.1002/bit.260190309. [PubMed] [Cross Ref]
13. Park JK, Chang HN. Microencapsulation of microbial cells. Biotechnol Adv. 2000;18:303–319. doi: 10.1016/S0734-9750(00)00040-9. [PubMed] [Cross Ref]
14. Farid MA, El Diwany AI, el-Enshasy HA. Production of oxytetracycline and rifamycins B and SV with cells immobilized on glasswool. Acta Biotechnol. 1994;14:67–74. doi: 10.1002/abio.370140112. [Cross Ref]
15. Adinarayana K, Ellaiah P, Srinivasulu B, Lakshmi Narayana J, Bapi Raju KVVSN. Optimization of medium and cultural conditions for neomycin production using response surface methodology.Ind J Biotechnol. In press.
16. Grove DC, Randall WA. Assay Methods of Antibiotics: A Laboratory Manual. New York, NY: Medical Encyclopedia, Inc; 1955.
17. Ministry for Health and Welfare, Government of India . Indian Pharmacopoeia. New Delhi, India: The Controller of Publications; 1996. pp. A–100.
18. Mahmoud W, Rehm HJ. Chlortetracycline production with immobilizedStreptomyces aureofaciens. I. Batch culture. Appl Microbiol Biotechnol. 1987;26:333–337. doi: 10.1007/BF00256664. [Cross Ref]
19. Ellaiah P, Murali Chand G, Srinivasulu B, Pardasaradhi SV. Production of cephalosporin C by immobilized cells ofCephalosporium acremonium. Indian J Exp Biol. 2000;38:1134–1137. [PubMed]

Articles from AAPS PharmSciTech are provided here courtesy of American Association of Pharmaceutical Scientists