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The effect of selected antioxidants (vitamin C, cysteine, fumaric acid) on the stability of a 0.3-mM solution of HTK has been determined. The stability of the HTK solution was determined using the changes in histidine content at elevated temperatures. The rate of the amino acid decomposition in this solution was 42.3% lower than in a solution without an antioxidant.
Vitamin C is the most effective antioxidant. An HTK solution stored at +5°C is stable for 450 days with vitamin C added to it, for 265 days with cysteine, for 242 days with fumaric acid, and for 260 days with no antioxidant.