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AAPS PharmSciTech. 2002 March; 3(1): 32–39.
Published online 2002 March 20. doi:  10.1208/pt030104
PMCID: PMC2750251

Statistical Modeling of protein spray drying at the lab scale


The objective of this study was to examine the effects of formulation and process variables on particle size and other characteristics of a spray-dried model protein, bovine serum albumin (BSA), using a partial factorial design for experiments. Formulation variables tested include concentration and zinc:protein complexation ratio. Process variables explored were inlet temperature, liquid feed rate, drying air flow rate, and atomizing nitrogen pressure on a lab-scale spray dryer. Statistical data analysis was used to determine F ratios for each of the inputs, which provided a means of ranking the importance of variables relative to one another for each powder characteristic of interest. It was found that protein concentration and atomizing nitrogen pressure had the greatest effects on the particle size of the protein powder. For determining product yield, results showed that protein concentration was the critical variable. Finally, the outlet temperature was mostly influenced by inlet temperature and liquid feed rate. Mathematical models based on these input-output relationships were constructed; these models provide insight into some of the controllable variables of the spray-drying process.

Key Words: Bovine serum albumin (BSA), Particle size, Spray drying, outlet temperature, Yield

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
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