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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
J Infect Dis. Author manuscript; available in PMC 2010 May 15.
Published in final edited form as:
PMCID: PMC2748061

Human Adenovirus 14a: A New Epidemic Threat

The recent much-publicized report World at Risk [1] predicts that we are soon to experience a biological or nuclear weapons attack. This issue of the Journal contains 2 reports of outbreaks of a new human adenovirus (Ad) type 14 strain [2, 3], which serves to remind us that we are at least equally likely, if not more likely, to soon experience large-scale morbidity through epidemics of emergent pathogens. As was illustrated by the severe acute respiratory syndrome-associated coronavirus, when a ubiquitous nuisance pathogen suddenly becomes more virulent, its reign of destruction needs little help from rogue nations or terrorist cells. Humankind is quite efficient in spreading such pathogens around.

It is likely that the new Ad14 strain entered Oregon in 2005 and apparently spread throughout the state by 2007 [2, 4]. In their retrospective review of clinical and laboratory data (from 1 November 2006 through 31 July 2007), Lewis et al. [2] demonstrate that the new Ad14 variant quickly became a highly prevalent strain, explaining 60% ofAd infections. Of 40 Ad14-positive patients, 76% required hospitalization, 61% merited supplemental oxygen, 47% received critical care, and 18% died. In a comprehensive contemporary epidemiological investigation in Texas, Tate et al. [3] show a similar rapid transmission of Ad14 with high morbidity. They estimate that, of 1147 otherwise healthy young military trainees with febrile respiratory illness in 2007, 551 (48%) were infected with Ad14. Of these Ad14-infected patients, 23 were hospitalized, 4 required admission to an intensive care unit, and 1 died. These transmission and morbidity statistics are consistent with those from the first report of this emergent Ad14 strain [4], when clusters totaling 140 patients with acute Ad14 respiratory disease were noted in Oregon, Texas, and Washington, with 38% being hospitalized, 17% requiring admission to an intensive care unit, and 9 dying.

The 52 human Ad serotypes [5] cause a broad spectrum of clinical illnesses: pharyngoconjunctival fever, keratoconjunctivitis, pneumonia, hemorrhagic cystitis, gastroenteritis, acute respiratory disease, severe disseminated disease, cardiomyopathy, and encephalitis [6]. Populations commonly at risk for adenoviral illness include new military recruits, young children, and especially those who are immunocompromised.

Ad14 (strain deWit) was first isolated from Dutch military recruits with acute respiratory disease in the 1950s and was associated with a few reported outbreaks of acute respiratory disease in Europe and Asia through the 1960s [713]. In the United States, Ad14 was only recently identified in military and civilian populations, has most often been associated with sporadic cases rather than outbreaks, and has not been previously associated with severe clinical illness [14, 15]. However, in 2005, Ad14 suddenly became more prevalent in the United States. In our 2004–2007 US National Surveillance for Emerging Adenovirus Infections program (3026 typed isolates in total) [14], we first detected Ad14 strains during March 2005 in Missouri and Arizona, and by April 2007 we had identified 19 Ad14 isolates from 12 different US laboratories. Similarly, Metzgar et al. [15] reported the abrupt emergence of Ad14 in US military populations in March and April 2006. Through further study, we now recognize that these recent Ad14 isolates are virtually identical to the emergent Ad14 genotype first reported to have caused serious illness among patients in New York, Oregon, Texas, and Washington during 2006–2007 [4]. As was described by both Tate et al. [3] and Lewis et al. [2], the Ad14 isolates detected in these outbreaks were identical by sequence data but were different from the Ad14 deWit strain [7]. On the basis of differences in restriction enzyme digest patterns, Louie et. al. designated the new strain Ad14a [16]. This new strain was observed to be associated with severe clinical illness more often than the prototype Ad14 deWit strain [4]. Novel Ad strains have been associated with more severe clinical disease and have seemed to have a competitive advantage in their spread [1721]. Although the genetic differences between the Ad14 deWit and Ad14a strains may account for the observed higher rate of transmission of Ad14a and its associated more severe morbidity, one can strongly argue that these observations are confounded by a lack of individual and herd immunity to Ad14 [2, 3, 16]. However, another observer might counter that Ad epidemics are not often seen when other rare Ad strains are detected. Virulence studies using cellular and animal models may be necessary to sort this out.

Fortunately, most Ad14a infections do not cause severe illness. Tate et al. [3] found that, of the trainees with serological evidence of acute Ad14a infection, 51% reported afebrile or mild disease, and 9% reported no respiratory symptoms at all. Even so, Ad14a represents an emerging threat in that it seems to cause more severe disease in some persons, has been implicated in community-based infections [2], and, in at least 1 facility, has demonstrated a propensity to infect hospital staff [22]. Data from reports on Ad14a suggest that risk factors for severe Ad14a disease may include male sex, older age, smoking, and underlying medical conditions, but more comprehensive study is needed [2, 3]. Available reports further suggest that Ad14a illness is mitigated by preexisting immunity [3, 15]. Tate et al. showed that previous natural infection with Ad7 may protect against severe Ad14a illness requiring hospitalization. This is particularly good news for military trainees, given the loss of Ad4 and Ad7 vaccines in 1999 and their expected return to availability soon [23, 24].

It is difficult to determine the geographical distribution of Ad14a infections, given that Ad surveillance is generally passive. Additionally, relatively few laboratories look for Ad, and even fewer can distinguish Ad14 from other Ad types. Through specific reports of Ad14a and our national surveillance program (for which sampling ended in 2007)[14], we know that Ad14a has been detected in Alaska, Arizona, California, Connecticut, Hawaii, Indiana, Missouri, New York, Oregon, Tennessee, Texas, Washington, and Wisconsin. With its propensity for rapid transmission, it seems likely that Ad14a is now circulating throughout the United States and may have been introduced from another country.

How does our knowledge of Ad14a affect future clinical care and public health? Given its association with more-severe disease, when Ad14a is detected in a medical facility, infection-control professionals may choose to employ patient isolation and special precautions to reduce the risk of nosocomial transmission. For patients with severe infections, clinicians, like those in Oregon [2], may be more aggressive in using antiviral therapy. Confronted with Ad14a outbreaks in crowded long-term-care facilities, public health officials may decide to employ nonpharmaceutical interventions [25]. Finally, should Ad14a prove to be a frequent cause of epidemics, those persons at greatest risk might benefit from the possible cross-protection conferred by Ad7 vaccine (although previous indications have included only military personnel).

Lewis et al. [2] describe polymerase chain reaction (PCR)-based methods to detect Ad14. Many other serological [5, 26], PCR-based [27], and DNA sequence-based typing methods [14, 2830] have been used to distinguish the 52 Ad strains. If Ad14 is detected in the United States, it most likely is the new Ad14a strain. However, restriction enzyme digest analysis or targeted gene sequencing is necessary to confidently distinguish the Ad14a strain from the Ad14 deWit strain [16].


Financial support: National Institute of Allergy and Infectious Diseases (grant 5R01AI053034-03 to support G.C.G.’s adenovirus research.


Potential conflicts of interest: G.C.G. recently served on Duramed Pharmaceuticals’ adenovirus vaccine data-monitoring committee. M.L.C. reports no potential conflicts.


1. Graham B, Talent J, Allison G, et al. World at Risk: The Report of the Commission on the Prevention of WMD Proliferation and Terrorism. New York: Vintage Books; 2008.
2. Lewis P, Schmidt MA, Lu X, et al. A Community-based outbreak of severe Human Adenovirus 14 respiratory illness. J Infect Dis. 2009 [PubMed]
3. Tate JE, Bunning ML, Lott L, et al. Outbreak of severe respiratory disease associated with emergent Adenovirus Type 14 at a United States Air Force Training Facility, 2007. J Infect Dis. 2009 [PubMed]
4. Acute respiratory disease associated with adenovirus serotype 14--four states, 2006–2007. MMWR Morb Mortal Wkly Rep. 2007;56:1181–4. [PubMed]
5. Jones MS, 2nd, Harrach B, Ganac RD, et al. New adenovirus species found in a patient presenting with gastroenteritis. J Virol. 2007;81:5978–84. [PMC free article] [PubMed]
6. Gray G, Chorazy M. Adenovirus. In: Yu V, Weber R, Raoult D, editors. Antimicrobial Therapy and Vaccines Volume I: Microbes (online) 3. 2008.
7. Van Der Veen J, Kok G. Isolation and typing of adenoviruses recovered from military recruits with acute respiratory disease in The Netherlands. Am J Hyg. 1957;65:119–29. [PubMed]
8. Bruj J, Farnik J, Sedmidubsky V. Epidemic of acute respiratory disease due to adenovirus type 14. Cesk Epidemiol Mikrobiol Imunol. 1966;15:165–71. [PubMed]
9. Cooper RJ, Hallett R, Tullo AB, Klapper PE. The epidemiology of adenovirus infections in Greater Manchester, UK 1982–96. Epidemiol Infect. 2000;125:333–45. [PubMed]
10. Kendall EJ, Riddle RW, Tuck HA, Rodan KS, Andrews BE, McDonald JC. Pharyngo-conjunctival fever; school outbreaks in England during the summer of 1955 associated with adenovirus types 3, 7, and 14. Br Med J. 1957;2:131–6. [PMC free article] [PubMed]
11. Mevzos LM, Il’ina TS, Makhmudov OS, Zolotarskaia EE, Dreizin RS. An outbreak of acute respiratory infections among adults caused by adenovirus serotype 14. Vopr Virusol. 1966;11:426–31. [PubMed]
12. Tai FH, Grayston JT, Johnston PB, Woolridge RL. Adenovirus infections in Chinese Army recruits on Taiwan. J Infect Dis. 1960;107:160–4. [PubMed]
13. Yamadera S, Yamashita K, Akatsuka M, Kato N, Inouye S. Trend of adenovirus type 7 infection, an emerging disease in Japan. A report of the National Epidemiological Surveillance of Infectious Agents in Japan. Jpn J Med Sci Biol. 1998;51:43–51. [PubMed]
14. Gray GC, McCarthy T, Lebeck MG, et al. Genotype prevalence and risk factors for severe clinical adenovirus infection, United States 2004–2006. Clin Infect Dis. 2007;45:1120–31. [PMC free article] [PubMed]
15. Metzgar D, Osuna M, Kajon AE, Hawksworth AW, Irvine M, Russell KL. Abrupt emergence of diverse species B adenoviruses at US military recruit training centers. J Infect Dis. 2007;196:1465–73. [PubMed]
16. Louie JK, Kajon AE, Holodniy M, et al. Severe pneumonia due to adenovirus serotype 14: a new respiratory threat? Clin Infect Dis. 2008;46:421–5. [PubMed]
17. Crawford-Miksza LK, Schnurr DP. Adenovirus serotype evolution is driven by illegitimate recombination in the hypervariable regions of the hexon protein. Virology. 1996;224:357–67. [PubMed]
18. Crawford-Miksza LK, Nang RN, Schnurr DP. Strain variation in adenovirus serotypes 4 and 7a causing acute respiratory disease. J Clin Microbiol. 1999;37:1107–12. [PMC free article] [PubMed]
19. Erdman DD, Xu W, Gerber SI, et al. Molecular epidemiology of adenovirus type 7 in the United States, 1966–2000. Emerg Infect Dis. 2002;8:269–77. [PMC free article] [PubMed]
20. Gray GC, Setterquist SF, Jirsa SJ, DesJardin LE, Erdman DD. Emergent strain of human adenovirus endemic in Iowa. Emerg Infect Dis. 2005;11:127–8. [PMC free article] [PubMed]
21. Gray GC. Adenovirus transmission--worthy of our attention. J Infect Dis. 2006;194:871–3. [PMC free article] [PubMed]
22. Yun HC, Prakash V. Transmission of adenovirus serotype 14 in the health care setting. Clin Infect Dis. 2008;46:1935–6. [PubMed]
23. Gray GC, Goswami PR, Malasig MD, et al. Adult adenovirus infections: loss of orphaned vaccines precipitates military respiratory disease epidemics. For the Adenovirus Surveillance Group. Clin Infect Dis. 2000;31:663–70. [PubMed]
24. Lyons A, Longfield J, Kuschner R, et al. A double-blind, placebo-controlled study of the safety and immunogenicity of live, oral type 4 and type 7 adenovirus vaccines in adults. Vaccine. 2008;26:2890–8. [PubMed]
25. Jefferson T, Foxlee R, Del Mar C, et al. Physical interventions to interrupt or reduce the spread of respiratory viruses: systematic review. Bmj. 2008;336:77–80. [PMC free article] [PubMed]
26. Malasig MD, Goswami PR, Crawford-Miksza LK, Schnurr DP, Gray GC. Simplified microneutralization test for serotyping adenovirus isolates. J Clin Microbiol. 2001;39:2984–6. [PMC free article] [PubMed]
27. Metzgar D, Osuna M, Yingst S, et al. PCR analysis of egyptian respiratory adenovirus isolates, including identification of species, serotypes, and coinfections. J Clin Microbiol. 2005;43:5743–52. [PMC free article] [PubMed]
28. Lu X, Erdman DD. Molecular typing of human adenoviruses by PCR and sequencing of a partial region of the hexon gene. Arch Virol. 2006;151:1587–602. [PubMed]
29. Sarantis H, Johnson G, Brown M, Petric M, Tellier R. Comprehensive detection and serotyping of human adenoviruses by PCR and sequencing. J Clin Microbiol. 2004;42:3963–9. [PMC free article] [PubMed]
30. Xu W, McDonough MC, Erdman DD. Species-specific identification of human adenoviruses by a multiplex PCR assay. J Clin Microbiol. 2000;38:4114–20. [PMC free article] [PubMed]