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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptNIH Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Arch Biochem Biophys. Author manuscript; available in PMC Sep 21, 2009.
Published in final edited form as:
PMCID: PMC2747633
NIHMSID: NIHMS136870
Special issue on transport ATPases
Giuseppe Inesi and Robert K. Nakamoto
Giuseppe Inesi, California Pacific Medical Center, Research Institute, 475 Brannan Street, San Francisco, CA 94107, USA Fax: +1 415 600 1725. E-mail address: ginesi/at/cpmcri.com;
Transport of solutes across plasma membranes and intracellular organelles is an absolute requirement for cell survival and homeostasis in all organisms, including bacteria, archaea, and eukaryotes. Transport of ions and metabolites against a concentration gradient requires free energy input, which is most commonly supplied by ATP. Enzymes that utilize ATP for transport of specific solutes are referred to as transport ATPases. These enzymes constitute a rather extensive family, subdivided into P, V, and ABC groups, according to their mechanism and the transported species. F-type ATPases that utilize electrochemical energy for formation of ATP are also considered transport ATPases (physiologically working in reverse). An increasing body of knowledge on the essential role of transport ATPases in functions such as signaling, homeostasis, and energy metabolism has developed. We have also learned of their relevance to diseases, including heart, mitochondria, cancer, osteoporosis, retinal degeneration, immune deficiency, cystic fibrosis, diabetes, gastric ulcer, nephrotoxicity, hearing loss, skin, copper-related disorders, lupus, and malaria. Compounds of pharmacological and therapeutic interest, and their interaction with transport ATPases, such as ouabain, thapsigargin, gastric H+ pump inhibitors, and the antimalarial artemisines, have been recognized. Furthermore, the important role of transport ATPases in the establishment of drug resistance has become evident.
Early discoveries of transport ATPases were followed by their physiological roles, cellular localization, pharmacology, definition of catalytic and transport mechanisms, molecular characterization of proteins, cloning and determination of sequences, heterologous expression, mutational analysis, and, more recently, structural resolution at the atomic level. From the conceptual standpoint, utilization of substrate (i.e., ATP) free energy was originally viewed as a requirement for chemiosmotic work, in order to overcome transmembrane concentration gradients and electrical charge imbalance. For instance, if we consider the sarcoendoplasmic reticulum (SERCA) Ca2+ ATPase, which is currently characterized in great detail, we would define the free energy required for its chemiosmotic work as
equation M1
reflecting the transmembrane Ca2+ concentration that can be achieved and the transmembrane electrical potential. This is certainly correct, but does not clarify the mechanism of transport. On the other hand, an initial mechanistic view of active transport was provided by Albers [1] and Post [2] or the Na+/K+ ATPase and by deMeis [3] for the Ca2+ ATPase, suggesting interconversion of these enzymes into two conformational states (E1 and E2) with varying affinities for bound cations. More recently, we learned that the catalytic and transport cycle of transport ATPases entails sequential steps, with defined equilibrium and kinetic constants, and a distinct conformational state of the protein in each sequential state. Large movements of structural domains are essential to the mechanism of transport coupled to substrate utilization. Returning again to the Ca2+ ATPase, it is now clear that rotation and bending of headpiece domains, in concomitance with ATP utilization, produce displacements of transmembrane segments including the Ca2+ sites. In fact, the conformational transition of the ground state (E1, with Ca2+ sites in high affinity and outward orientation) to the phosphorylated state (E2-P, with Ca2+ sites in low affinity and inward orientation) can defined as
equation M2
and
equation M3
The energy required for this transition can then be viewed approximately as
equation M4
which is contributed by the ATP γ-phosphate under standard conditions. Analogous considerations, in reverse, can be made regarding the utilization of electrochemical energy by F-type ATPases, where rotatory motion induced by H+ flux is followed by long-range displacement of protein subunits and conformational transitions, affecting the nucleotide and Pi binding site to favor formation of ATP. These considerations indicate that the free energy of ATP is in fact utilized for conformational work, which is central to the mechanism of active transport. Therefore, in addition to their enzymatic function as chemical catalysts, the ATPases protein molecules undergo long-range conformational changes that are essential mechanistic linkages to the coupling of catalysis and active transport. Demonstration of protein conformational changes maybe considered a basic conceptual advance that applies to the mechanism of all transport ATPases. Additional discoveries on specific structural features, binding modalities of transported species and ATP, covalent chemistry, stoichiometry, and charge movements are currently advancing our knowledge on the mechanism of each specific ATPase.
In this special issue of ABB, rather than providing an exhaustive list, we endeavored to collect descriptions of selected ATPases, representative of each group, and most useful for explaining general as well as specific features of the structure and function of these enzymes. We also attempted to expose the main methodological approaches used in the field, such as detailed functional characterization, high-resolution structural studies, recombinant and mutation experiments, microscopic detection of charge movements, theoretical analysis, and demonstration of relevance to diseases. We hope that this issue will be useful to scientists who are interested in transport ATPases, as well as for teaching or pursuing graduate courses in biochemistry, biology, biophysics, pharmacology, physiology, and generally medical sciences.
Footnotes
This article appeared in a journal published by Elsevier. The attached copy is furnished to the author for internal non-commercial research and education use, including for instruction at the authors institution and sharing with colleagues. Other uses, including reproduction and distribution, or selling or licensing copies, or posting to personal, institutional or third party websites are prohibited. In most cases authors are permitted to post their version of the article (e.g. in Word or Tex form) to their personal website or institutional repository. Authors requiring further information regarding Elsevier's archiving and manuscript policies are encouraged to visit: http://www.elsevier.com/copyright
Contributor Information
Giuseppe Inesi, California Pacific Medical Center, Research Institute, 475 Brannan Street, San Francisco, CA 94107, USA Fax: +1 415 600 1725. E-mail address: ginesi/at/cpmcri.com.
Robert K. Nakamoto, Department of Molecular Physiology and Biological Physics, University of Virginia, P.O. Box 800736, Charlottesville, VA 22908−0736, USA Fax: +1 434 982 1616. E-mail addresses: rjknakamoto/at/virginia.edu.
References
1. Albers RW. Annu. Rev. Biochem. 1967;36:727–756. [PubMed]
2. Post RL, Hegyvary C, Kume S. J. Biol. Chem. 1972;247:6530–6540. [PubMed]
3. De Meis L, Viann AL. Annu. Rev. Biochem. 1979;48:275–292. [PubMed]