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J Invest Dermatol. Author manuscript; available in PMC Sep 17, 2009.
Published in final edited form as:
PMCID: PMC2745834
NIHMSID: NIHMS112671
Evaluation of the Clonal Origin of Multiple Primary Melanomas Using Molecular Profiling
Irene Orlow,1 Diana Tommasi,1 Bradley Bloom,1 Irina Ostrovnaya,1 Javier Cotignola,1 Urvi Mujumdar,1 Klaus J. Busam,2 Achim A. Jungbluth,3 Richard A. Scolyer,4 John F. Thompson,4 Bruce K. Armstrong,5 Marianne Berwick,6 Nancy E. Thomas,7 and Colin B. Begg1
1Department of Epidemiology and Biostatistics, Memorial Sloan-Kettering Cancer Center, New York
2Department of Pathology, Memorial Sloan-Kettering Cancer Center, New York
3Ludwig Institute for Cancer Research, New York Branch, Memorial Sloan-Kettering Cancer Center, New York
4Sydney Melanoma Unit, Royal Prince Alfred Hospital, Sydney, Australia
5University of Sydney and Cancer Council New South Wales, Sydney, Australia
6University of New Mexico, Albuquerque, NM
7University of North Carolina, Chapel Hill, North Carolina
Corresponding Author: Colin B. Begg, Department of Epidemiology and Biostatistics, Memorial Sloan-Kettering Cancer Center, 307 East 63rd St, New York, NY 10065. Phone: (646) 735-8108, Fax: (646) 735-0009, beggc/at/mskcc.org
Numerous investigations have been conducted using molecular profiling to evaluate the possible clonal origin of second malignancies in various cancer types. However, to date no study assessing clonality of multiple primaries has been conducted in melanoma. In this investigation using patients treated at a specialist melanoma treatment center, we compared the somatic mutational profiles of pairs of melanomas designated as independent on the basis of thorough assessment of their clinical and pathologic characteristics. We used a set of highly polymorphic genetic markers selected on the basis of their chromosomal positions and the frequencies of reported allelic losses at these genetic loci. Our statistical testing strategy showed no significant evidence of clonal origin of the two primaries in 17 of the 19 patients examined. The results suggest that most second melanomas designated as independent primary tumors on the basis of their clinicopathologic features are indeed independent occurrences of the disease, supporting the validity of the criteria used by experienced pathologists in distinguishing new primaries from metastases.
Keywords: Melanoma, Clonality, Loss of Heterozygosity, Diagnosis
In recent years many investigative studies using new molecular technologies have sought to distinguish independent primary cancers from metastases in a more definitive manner than is possible by routine assessment of clinical and pathologic features. These have been conducted in various organ systems using molecular profiling of cells from pairs of tumors from individual patients, and a large literature of these studies has developed, most prominently in the area of head and neck cancer (Ha and Califano, 2003) and bladder cancer (Hafner et al, 2002), two sites where second malignancies are common. Typically, this has involved examination of the tumors for somatic mutations in genes that are frequently altered in cancers of the type under investigation, by examining microsatellite instability or loss of heterozygosity (LOH) at mutational hot spots where LOH occurs frequently. The similarities of the genetic events in both tumors are then examined to determine whether they appear to be closely matched. If so, the tumors are considered to be “clonal”, that is deriving from a single cell that experienced the pivotal mutations prior to seeding both tumors.
Molecular studies of clonality have been prominent in understanding the development of smoking-related aerodigestive cancers. The concept of field cancerization postulates distinct tumors developing independently due to a common, regional exposure to the carcinogen (Slaughter et al, 1953). Molecular studies, however, have demonstrated that frequently these subsequent primaries are in fact clonally related (Worsham et al, 1995; Bedi et al, 1996; Scholes et al, 1998). A contrasting picture emerges from studies of contralateral cancer of the breast and lung. Authors of these studies have generally reached the conclusion that the tumors are typically independent for contralateral breast cancers (Kollias et al, 2000; Janschek et al, 2001; Stenmark-Askmalm et al, 2001; Imyanitov et al, 2002; Tse et al, 2003; Chunder et al, 2004; Regitnig et al, 2004; Schlechter et al, 2004), although corresponding studies of new ipsilateral breast cancers indicate that these are predominately of clonal origin (Goldstein et al, 2005a; 2005b). Studies in lung cancer have been conducted using microsatellite markers to distinguish microsatellite instability (MSI) (Leong et al, 1998; Huang et al, 2001; Shin et al, 2001; Dacic et al, 2005; Geurts et al, 2005) and several have tested mutations in TP53 and/or K-ras (Sozzi et al, 1995; Lau et al, 1997; Hiroshima et al, 1998; Holst et al, 1998; Matsuzoe et al, 1999; Shimizu et al, 2000; Shin et al, 2001; van Rens et al, 2002; Murase et al, 2003). These studies have evaluated clonality in a range of clinical settings, including the comparison of synchronous or metachronous multiple primaries, comparisons of primaries with metastatic tumors, and comparison of head and neck primaries with solitary lung nodules that may or may not be metastases. The results of these studies are mixed, but similarly to breast cancer the evidence appears to suggest that contralateral lung tumors are predominantly of independent origin.
Our study was stimulated by the importance of this issue for interpreting findings from epidemiologic studies of melanoma. Melanoma is a relevant model for the study of clonality because the reported frequency of second primary melanoma is high: melanoma patients experience a rate of occurrence of melanoma about 7-8 times greater than the age-matched general population (Begg, 2001). Furthermore, it is not uncommon for individual patients to develop several primary melanomas. These patients provide a rich potential resource for cancer epidemiologic research (Neugut et al, 1999). Patients with second primaries are increasingly used in epidemiologic case-control studies (see for example Millikan et al, 2005; Berwick et al, 2006; Kanetsky et al, 2006; Orlow et al, 2007; Concannon et al, 2008). Risk factors occur with greater frequency in these patients than in patients with a single malignancy or in population controls. As a consequence epidemiologic studies using second primaries can possess greatly enhanced statistical power compared to conventional studies, especially for the study of rare, highly penetrant genetic risk factors (Begg and Berwick, 1997). These types of studies rely on the assumption that individuals recruited on the basis of the diagnosis of a second primary tumor have truly experienced a cancer diagnosis twice (Begg et al, 2006). However, it is plausible that a significant subset of these second and higher-order primaries are actually clonal recurrences of the initial primary tumor, mis-diagnosed as independent second primaries.
There are several criteria for classifying a new melanoma as an independent primary. The strongest evidence in favor of a primary tumor is the presence of an associated precursor lesion (melanocytic nevus or in situ melanoma). Additional criteria to differentiate metastatic and primary lesions include location, grouping, invasion of lymphatic capillaries, and presence of a brisk inflammatory cell infiltrate, although some of these characteristics may be shared by both primaries and metastatic melanomas (Heenan and Clay, 1991; Bengoechea-Beeby et al, 1993). For pathologists familiar with the spectrum of pathologic features of melanocytic tumors it is usually not difficult to establish a pathologic diagnosis of primary cutaneous melanoma, particularly if the diagnosis is made in the context of an appropriate clinical history. However, it can be difficult or even impossible to determine whether a melanoma is a primary tumor or a metastasis on the basis of histologic characteristics alone (Guerriere-Kovach et al, 2004). This is particularly the case for melanomas involving the dermis devoid of an in situ component in the overlying epidermis or other associated precursor lesion. Such tumors may be diagnosed incorrectly as metastatic melanoma on pathologic assessment. Conversely, some metastatic melanomas can show prominent epidermotropism, mimicking a primary tumor (Abernethy et al, 1994; White and Hitchcock, 1998; Swetter et al, 2004). In some instances the clinical features may be the only clues to the recognition that the tumor is, in fact, a metastasis.
In the light of these issues it is surprising that the clonal relationship between first and second primary melanomas has not been previously investigated using molecular techniques. Clonality has been examined for “in-transit” melanoma metastases by investigating loss of heterozygosity (LOH) at 8 candidate loci in the primary tumors and the lymphatic metastases, demonstrating close concordance of the genetic fingerprints of lesions derived from the same patient (Nakayama et al, 2001). A more recent study compared X-chromosome inactivation and LOH in five loci between primary melanomas and their corresponding metastases, and the results revealed that the majority of melanoma metastases share a common clonal origin with the matched primary tumor (Katona et al, 2007). Furthermore, a group of investigators led by Bastian has conducted a series of studies examining copy number changes in melanomas and benign nevi using array CGH techniques (Bastian et al, 1998; 1999; 2000; 2003; Curtin et al, 2005). They showed that the benign nevi exhibited very few copy number abnormalities relative to the malignant tumors, confirming the potential value of molecular profiling as a diagnostic tool in differentiating benign from malignant melanocytic tumors. However, to our knowledge, no studies have been conducted that seek to challenge the validity of the diagnosis of new primary melanomas as independent occurrences of cancer.
Determining whether a melanoma is a primary or a metastasis is of critical clinical importance. In contrast to a new primary, metastatic disease is rarely curable. Furthermore, primary melanomas and melanoma metastases are managed clinically in quite different ways. Also, as noted above, the distinction is important for the validity of epidemiologic studies of multiple primary cancers.
We compared the mutational profiles of pairs of presumptively independent primary melanomas for each of a series of 19 patients who had been treated at the Sydney Melanoma Unit, Royal Prince Alfred Hospital in Sydney, Australia. These comparisons were on the basis of 26 highly polymorphic markers (Table 1). Loss of heterozygosity (LOH) is represented in the table by black triangular symbols, with the direction of the symbol distinguishing losses on the short versus long allele. Thus, concordant black triangles indicate losses of the same allele at the same locus, and represent potentially clonal mutations, though clearly such concordances could occur independently on the two tumors by chance. Likewise, independent mutations could occur in either tumor even if the tumors shared a clonal origin. To assess the evidence favoring clonality, we used a statistical test that determines whether the number of concordant mutations exceeds the number expected on the basis of chance.
Table 1
Table 1
Mutational patterns observed in patients with multiple primary melanomas
For most of the cases the patterns of LOH appear to be random. The results of the statistical tests displayed at the bottom of the table indicate that only 2 of the 19 cases have statistically significant evidence of clonal relatedness, p=0.01 for case #34, and p=0.04 for case #30. Since we used a statistical test with a significance level of 5% we expect one “significant” finding when we perform about 20 independent tests. Case #34 has relatively few mutations, 3 on the first tumor (T1) and 2 in the second tumor (T2), with the two common mutations occurring on the same allele. Case # 30 showed genetic alterations in both tumors for 7 of the markers, with 6 of these 7 occurring on the same allele. Interestingly, case #34 involved two synchronously occurring melanomas, both on the trunk, and both superficial spreading melanomas (clinical details of all cases are provided in Table 3). In contrast, case #30 involved tumors that occurred 2.4 years apart in distinct anatomic locations, and with different cell types. These data suggest that most of these tumor pairs are independent, confirming the pathologic diagnoses, though we cannot rule out the possibility that one or two are clonal.
Table 3
Table 3
Clinical and histologic characteristics of multiple melanomas
To verify that our testing procedure has the potential to detect tumor pairs whose origin is clonal we also examined 13 metastatic tumors from 5 patients (one patient had 4 metastases, another had 3), obtained from archival material from Memorial Sloan-Kettering Cancer Center in New York. As shown in Table 2, ten of the 12 comparisons of these definitively clonal pairings demonstrated statistically significant evidence of clonal relatedness (sensitivity = 83%), with 8 of the pairings producing strongly significant (P<0.01) findings.
Table 2
Table 2
Mutational patterns observed in controls with metastases
Although a number of studies of the possible clonal origin of double malignancies have been conducted to date, none to our knowledge have involved double primary melanomas. This absence may be due to the fact that most dermato-pathologists do not perceive the misdiagnosis of a metastasis as a second primary as a likely occurrence or as a diagnostic problem. However, the high incidence of reported multiple primaries in this disease could be due in part to the misdiagnosis of metastases as independent primaries. Our study was constructed to provide preliminary evidence on this issue. The results would appear to support the conclusion that most second primary melanomas diagnosed on the basis of their clinical and pathologic characteristics are indeed independent occurrences of the disease.
It is of interest to examine more closely the two cases that showed patterns suggestive of clonal relatedness. Case #30 exhibited concordant LOH at 6 separate genetic loci, yet the tumors have different cell types, occurred 2.4 years apart, and were located in distant anatomic sites. A re-inspection of the pathological characteristics indicated that both tumors had significant epidermal components extending beyond bulky dermal components. Case #34 had only two concordant mutations, but the overall patterns were very similar, that is most of the loci exhibited no mutations on either tumor. The two tumors were synchronous, with the same cell type in the same general anatomic site, the chest, although the tumors were in the left and right portions of the chest, and well apart. Re-examination of the pathology in recut sections showed that the two tumors were mostly epidermal, and thus appeared pathologically to be independent primaries. Since we expect one false positive finding for every 20 statistical tests performed at the 5% significance level, the observation of only 2 significant results in this set of 19 is broadly consistent with the conclusion that few, if any, of these melanoma pairs, and very few in general, are of clonal origin.
Our study has technical, epidemiological and statistical limitations. We obtained specimens from both primaries for 19 cases, but these cases were selected based on the availability of sufficient tissue samples. This opportunistic selection of cases, and the small sample size, limits our ability to estimate accurately the proportion of cases that may be mis-diagnosed. Furthermore the cases were obtained from a specialized melanoma treatment center where the pathologic reviews were accomplished by dermato-pathologists specializing in melanoma, and where full clinical histories were also available. All such information is rarely available to either clinicians or pathologists at the time of initial diagnosis in routine clinical practice and hence misdiagnosis of a metastasis as a primary may be somewhat more common in everyday clinical practice, particularly outside of specialist centers. Nonetheless, most patients with a second skin melanoma designated as a second primary have clinical courses consistent with a new primary and more favorable than would be expected for stage IV melanomas. For a subset of markers we encountered amplification failures. This could be a result of primers being unable to anneal to their specific sequences due to homozygous deletions or duplications, but more likely the high rate of failures encountered for D2S131, D2S2291, D6S275, D6S457, D10S185 and D13S153 was due to the suboptimal quality of the DNA.
Melanoma is a disease that is frequently characterized by small tumors. We had hoped as part of this study to conduct array comparative genomic hybridization on all pairs of samples as an alternative genomic approach to profiling the tumors. However, sufficient DNA of high molecular weight suitable for array CGH for both tumors in the pair was available only for 4 cases (data not shown). In general, for a technology of this nature to be applicable in a clinical diagnostic setting, we would need a minimum of 0.6μg of high molecular weight DNA from each tumor and counterpart normal sample if extracted from fresh frozen tissue, or 1.5μg of DNA extracted from formalin-fixed, paraffin-embedded tissue. Such quantities will typically not be available from both tumors. With a polymerase chain reaction (PCR)-based method such as the one presented here, one would require no more than 0.5μg of DNA if testing a relatively high number of microsatellite repeats, and approximately 0.2μg when working with fresh-frozen tissue.
We employed a statistical test that was designed specifically for the purpose of detecting clonal relatedness (Begg et al, 2007). This test is based on the simplifying assumptions that the mutations at different loci are independent, that the probabilities of mutations are similar for each locus, and that each allele is equally likely to experience a mutation. Each of these assumptions is clearly approximate. Validation studies show that the test is robust to modest departures from the latter two assumptions (Begg et al, 2007). In fact, in our presumptively independent cases 66% of the losses that occurred in both members of the tumor pair occurred on the same allele. This modest preponderance of concordances could be the result of clonality in some of the pairs (such as cases 30 and 34), but it may also be explained by the possibility that allelic changes do not occur with equal probability for the alleles at specific genetic loci, especially if located within or nearby a gene involved in the development of the tumor. If this is true then we expect to see a modest correlation in mutational profiles even for independent tumors. The statistical power of the test is, of course, dependent on the number of independent genetic markers evaluated. In practice one could increase power by examining more loci for allelic gains and losses, and by testing for presence of common point mutations such as the V600E variant on the BRAF gene.
In summary, our study provides evidence that most melanomas that are classified as independent second primaries on the basis of comprehensive clinicopathologic analysis in a specialist melanoma treatment center are indeed independent occurrences of melanoma. In clinical use, this technology could, on present evidence, be a supplement to but not a replacement for detailed clinical and pathologic evaluation of the lesions.
Case Selection
Archival specimens of sufficient quality for analysis were obtained from two independent primary melanomas for each of a series of 19 patients who had been treated at the Sydney Melanoma Unit, Royal Prince Alfred Hospital in Sydney, Australia. These cases were selected on the basis of the availability of specimens from both tumors with dimensions (based on diameter and thickness) that were likely to provide sufficient DNA for analysis. Clinical and pathologic details are reported in Table 3. In 13 patients the tumor pairs occurred in the same general anatomic region and in 16 pairs the tumors were of the same histologic type. In 10 of these patients, the lesions mapped both to the same anatomic region and had the same histologic subtype. For comparison, we also utilized 12 “known” metastatic lesions in 5 patients with melanoma available as archived material at the Memorial Sloan-Kettering Cancer Center in New York. One patient had 4 synchronous tumors to the leg (control #2, Table 2), while another patient had three related tumors (control #1). The study was approved by the Institutional Review Boards at the Royal Prince Alfred Hospital and Memorial Sloan-Kettering Cancer Center. The study was conducted according to Declaration of Helsinki Principles.
Marker Selection
We chose 26 highly polymorphic genetic markers; these were selected on the basis of their chromosomal positions and their reported or expected allelic loss (Table 4) (Thompson et al, 1995; Nakayama et al, 2001; Shirasaki et al, 2001; Massi et al, 2002; Pollock et al, 2003; Uribe et al, 2005). Eleven of these markers map to 8 different chromosomes and have previously shown a high incidence of LOH or MSI (>30%) either in primary or metastatic melanomas (35,46-48): D1S214 (1p36.3), D2S2182 (2p16), D2S2291 (2p16), D6S275 (6q15-q16), D6S457 (6q21-q23.2), D9S304 (9p21), D9S157 (9p23-p22), D10S212 (10q26.12-13), D11S2000 (11q22-q23), D13S153 (13q14), D17S786 (17p13), and D17S1322 (17q21). The heterozygosity of these markers ranged from 20% to 62% in published studies (Bengoechea-Beeby et al, 1993; Thompson et al, 1995; Shirasaki et al, 2001; Pollock et al, 2003;) and from 61% to 92% according to the Centre d’Etude du Polymorphisme Humain (CEPH) database (version v2.1 last accessed on April 8th 2008). The following 6 markers with heterozygosities between 69% and 87% (CEPH) have not been previously tested in melanomas but were selected because they map to chromosomal arms found by Curtin et al (2005) to be altered : D6S1043 (6q16), D7S1824 (7q34), D8S1104 (8p11), D10S676 (10q22), D11S1998 (11q23), and a pentanucleotide repeat within the TP53 gene (17p13). An additional set of 5 markers on 3 different chromosomes showed 19% to 23% LOH in melanoma cases as reported by Uribe and colleagues (Uribe et al, 2005): D10S185 (10q23.3), D2S139 (2p12), D2S131 (2p22-25), D2S206 (2q33-37). Finally, 4 more markers were selected that were at known or suspected oncogene or tumor suppressor gene sites. These markers are: D4S1543 on 4q13 (c-Kit maps to 4q11-12), D1S2882 and D1S2766 which map to the smallest overlapping deletion (SRO1) suspected to harbor a new melanoma tumor suppressor gene (Walker et al, 2004), and D3S1293 on 3p22 which maps near TGFBR2 (Nakayama et el, 2001), with heterozygosities between 67% and 74% (CEPH).
Table 4
Table 4
Microsatellite markers for the study of melanoma clonality
Sample Preparation and DNA extraction
DNA was extracted from the tumor area contained in 20 to 30 × 5μm-thick formalin-fixed paraffin embedded tissue sections placed on uncharged glass slides. A hematoxylin-eosin stained slide was used to confirm the presence of tumor and to differentiate between tumor and normal adjacent cells. These areas were then isolated and scraped into separate Eppendorf tubes with sterile scalpels. Tissues were deparaffinized with xylene and DNA extracted with the QIAamp Micro Kit (Qiagen Inc., Valencia, CA) following the manufacturer’s recommendations. The DNA quantity and quality were determined by measuring the A260, A280, and A230 with a NanoDrop ND1000 spectrophotometer (Nanodrop, Wilmington, DE).
Polymerase Chain Reaction (PCR) and Fragment Size Analysis
Analyses of microsatellites were performed by PCR using primers flanking the repetitive sequence, coupled with fragment size analysis using a fluorescent label. During assay design, all primer pairs were checked with the Basic Local Alignment Search Tool (Blast, NCBI) to ensure specificity. Specific fragments were amplified in a reaction mix containing 10 to 15ng DNA, 0.5 μM each of the specific forward and reverse primers, 300 μM dNTP, 0.05 U/μl DNA Polymerase, and AmpliTaq Buffer II containing 1.5 mM MgCl2 (Applied Biosystems, Foster City, CA). Specific primer sequences amplified products of 103 to 247bp and are listed, together with the cycling conditions, in Table 5. After amplification, the products were loaded onto 2.5% agarose gels stained with ethidium bromide, and examined after electrophoresis. The quantity of PCR product obtained was assessed by comparing the band intensities to a mass marker (Invitrogen, Carlsbad, CA). PCR products were diluted to 1-3ng/μl and then analyzed by capillary electrophoresis on the ABI 3730xl DNA sequencer (Applied Biosystems, Foster City, CA) in the presence of a GS500LIZ size standard (Applied Biosystems) and by use of the GeneScan ver3.0 software (Applied Biosystems) to determine product length. The electropherograms were analyzed with Peak Scanner v1.0 software (Applied Biosystems) and the ABI PRISM® GeneMapper TM Software version 3.0. Samples were considered informative when two clear allelic peaks were present in the electropherograms of the normal DNA (heterozygous sample), and not informative when only one peak was present (homozygous sample). For the informative sets, the ratios of allele 1 and allele 2 - signals were compared in normal (N) and tumor tissue (T) [(Nallele1/Nallele2: (Tallele1/Tallele2). This ratio should be close to 1 when no allelic loss has occurred. We note that since the PCR-based microsatellite analysis consists of examination of the relative allelic peak heights, in several instances we cannot distinguish between loss of an allele and gain of the contralateral allele. However, most of the markers used map to chromosomal arms deleted in melanoma (Thompson et al, 1995; Bastian et al, 1998) and therefore all allelic changes were designated as losses of heterozygosity (LOH). The cutoff to establish whether LOH had occurred was chosen based upon microscopical evaluation of the H&E stained tissues and considered on a case-to-case basis. As an example, if the tumor sample contained ~20% normal cells, LOH was defined as a 40% reduction or more in the intensity of one of the two alleles in the tumor sample (Figure 1) (Orlow et al, 1994).
Table 5
Table 5
Experimental conditions used for the detection of allelic losses and gains
Figure 1
Figure 1
Analysis of allelic gains and losses using fragment size analysis (FSA). The depicted results correspond to sets of electropherograms obtained for the tetranucleotide-repeat marker D7S1824 for patients in which the tumor pairs showed concordants allelic (more ...)
Quality control
Careful labeling of study samples and 96-well plates was monitored throughout all procedures. To avoid contamination, DNA extraction and pre-PCR procedures including scraping of cells from the paraffin embedded tissue were conducted in areas free of PCR products and with dedicated instrumentation, including aerosol resistant pipette tips and disposable plastic ware. Pipettes were wiped with ethanol and exposed together with plastic ware to UV for 15 minutes before each use. Samples that failed to amplify were repeated at least twice. All results were interpreted at least twice by two laboratory members (D.V.T., I.O.)
Statistical Analysis
The patterns of mutational events in the two tumors were compared using a statistical test designed for this specific purpose (Begg et al, 2007). The test involves counting the total number of concordant mutations that occur on the same parental allele, and benchmarking this total against a reference distribution that is based on the assumption that mutations on the two tumors occurred randomly.
Acknowledgments
We thank Bushra Zaidi for her assistance with the hematoxylin-eosin staining; Juan Li for her help with the GeneMapper software during the evaluation of fragment sizes; and Stacey Yang for her assistance in locating tissue blocks and original pathology reports for multiple primary melanomas.
Supported by the National Cancer Institute, Awards CA125829, CA124504 and CA020449-29, the Melanoma Foundation of the University of Sydney, the Cancer Institute New South Wales, the Australian National Health and Medical Research Council and the Memorial Sloan-Kettering Cancer Center Cancer Education Program.
Abbreviations
LOHLoss of Heterozygosity
PCRPolymerase Chain Reaction

Footnotes
CONFLICT OF INTEREST The authors declare no conflict of interest.
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