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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
J Am Chem Soc. Author manuscript; available in PMC 2010 August 19.
Published in final edited form as:
PMCID: PMC2745615
NIHMSID: NIHMS135254

A New Gadolinium-based MRI Zinc Sensor

Abstract

The properties of a novel Gd3+-based MRI zinc sensor are reported. Unlike previously reported Gd3+-based MRI contrast agents, this agent (GdL) differs in that the agent alone binds only weakly with human serum albumin (HSA) while the 1:2 GdL:Zn2+ ternary complex binds strongly to HSA resulting in a substantial, three-fold increase in water proton relaxivity. The GdL complex is shown to have a relatively strong binding affinity for Zn2+ (KD = 33.6 nM), similar to the affinity of the Zn2+ ion with HSA alone. The agent detects as little as 30 μM Zn2+ in the presence of HSA by MRI in vitro, value slightly more than the total Zn2+ concentration in blood (~20 μM). This combination of binding affinity constants and the high relaxivity of the agent when bound to HSA suggests that this new agent may be useful for detection of free Zn2+ ions in vivo without disrupting other important biological processes involving Zn2+.

Introduction

Zinc is the second most abundant trace element (after iron) in mammalian tissues (the average adult human has ca. 3 grams of zinc).1 Zinc exists exclusively as a divalent ion (Zn2+) in biology with most Zn2+ bound to proteins that play a pivotal role in controlling gene transcription and metalloenzyme function.2 Today, there is broad awareness of Zn2+ signaling with a dozen or more individual zinc-secreting cell types known.3 These include cells in the submandibular salivary gland, the pancreatic β-cells and pancreatic exocrine cells, the prostate epithelial cells, Paneth cells in the intestine, mast cells, granulocytes, pituitary cells and CNS neurons.4 Zn2+ is present in particularly high concentrations in the mammalian prostate, pancreas and brain. In addition to modulating neuronal transmission in brain, Zn2+ is reported to contribute to neuronal injury in certain acute conditions, to suppress or induce apoptosis, and to induce the formation of β-amyloid (βA),5 which is reported to be related to the etiology of Alzheimer's disease.6,7 A considerable amount of data now shows that Zn2+ and other metal ions (iron and copper) contribute to formation of βA deposits in the brain.8 Concentrations as high as 0.2 to 1 mM of Zn2+ have been found in amyloid plaques. It is also known that pancreatic β-cells release free chelatable Zn2+ simultaneously with release of insulin such that the local concentration of Zn2+ surrounding activated β-cells may be as high as 0.48 mM.9 Thus, monitoring release of Zn2+ from β-cells in vivo is an important goal in understanding the etiology and treatment of diabetes.10

Many disease conditions associated with either high or low Zn2+ concentrations are still widely debated and although it is recognized that Zn2+ has many important cellular roles, its physiological significance is not completely understood. Several chemical Zn2+ sensors have been reported, most based on fluorescence detection.11 While these have proven useful for monitoring Zn2+ fluctuations in cells, their in vivo application is limited by light penetration in tissue, light scattering, and toxic photobleached byproducts. To the best of our knowledge, detection of free Zn2+ in vivo has not been demonstrated to date. Among alternatives to optical methods, magnetic resonance imaging (MRI) is promising since this technique allows non-invasive high resolution imaging of opaque bodies and monitoring of dynamic processes. There is considerable interest in the development of MRI contrast agents (CAs) that respond to biological events.12 Most MR contrast agents are based on gadolinium (Gd3+) complexes that increase the relaxation rate of water (r1) through exchange of a single Gd3+-bound water molecule with bulk solvent. The design of responsive CAs is typically based on changes in one or more factors that control proton relaxation times - water access to the metal, rotational correlation times, metal bound water exchange rates - in response to a specific stimulus (eg. enzyme,13 protein,14 pH,15 metal,16 etc.). A few Zn2+-specific MRI CAs have been reported previously. The first was reported by Nagano and coworkers17 who prepared a Gd3+ complex based on the diethylenetriaminepentaacetic acid (DTPA) ligand bifunctionalized with N,N-bis(2-pyridyl-methyl) ethylene diamine (BPEN), a moiety that has shown to have a binding specificity for Zn2+ over other metals. The relaxivity of this complex decreased from 6.1 mM−1 s−1 to 4.0 mM−1 s−1 (25°C, pH 8, 7T) in the presence of 1 equivalent of Zn2+, presumably by restricting access of water to the Gd3+ coordination site. However, the relaxivity of this complex returned to its initial value when a second Zn2+ was bound so these features make this system unattractive for testing in vivo. The same authors also reported a modified version of the DTPA derivative wherein one of the pyridylmethyl groups was replaced by an acetate moiety. This resulted in a complex that responded to Zn2+ by changing its relaxivity from 4.8 to 3.5 mM−1 s−1 (25°C, pH 7.2, 7T) upon addition of one equivalent of Zn2+ with no further changes upon addition of a second equivalent of Zn2+.18 Although this was an improvement in one sense, both systems result in an “on-off” response or image darkening upon Zn2+ binding – clearly not an optimal situation. More recently, Meade and coworkers 19,20 reported an “off-on” Zn2+-responsive agent derived from GdDO3A. In this agent, the metal water coordination site is occupied by a carboxylate group of a diaminoacetate moiety attached through the fourth amino group of the macrocycle. When the diaminoacetate binds Zn2+, a water molecule then coordinates to the Gd3+ which translates in an increase in relaxivity from 2.3 to 5.1 mM−1 s−1 (37°C, pH 7.4, 60MHz), a much more favorable 120% change. When dissolved in blood serum, however, the change in relaxivity of this complex changed much less in response to Zn2+, from 5.8 to 7.7 mM−1 s−1. Nevertheless, an in vitro study showed that 100 μM Zn2+ could be detected by MRI by this agent in buffer and a Zn2+ binding constant of 240 μM was determined by using a fluorescence competition method. Given that one would like to detect Zn2+ at even lower levels in vivo, continued development of agents that respond to Zn2+ in vivo under physiological conditions is highly desirable.

Results and discussion

Gd3+-based MRI contrast agents derived from DOTA-like macrocyclic ligands have advantages over their linear counterparts (DTPA) because of their higher thermodynamic stability and kinetic inertness. Previous work has shown that the GdDTPA-bisBPEN diamide agent (zero net charge) developed by Nagano forms a 1:2 complex with Zn2+ while the EuDOTA-bisBPEN-di-butyl tetraamide PARACEST sensor (+3 charge) only forms a 1:1 complex with Zn2+.21 These differences must reflect the differences in total charge on each Ln3+ complex. Given these trends, a decision was made to investigate the Zn2+ binding properties of GdDOTA-bisBPEN diamide (net positive charge), 3.

3 was prepared as outlined in Scheme 1. 1 was first obtained by using a previous published procedure following protection-derivatization-deprotection schemes.22 1 was then coupled with 2 equivalents of BPEN (2) via activation of the free carboxylic acids with HBTU. After product purification, the tert-butyl groups were removed yielding 3 in a 74% overall yield. The Gd3+ and Eu3+ complexes of 3 were prepared and further purified by HPLC.

Scheme 1
i) HBTU, DMF, ii) TFA, DCM.

The relaxivity of GdDOTA-diBPEN increased with addition of Zn2+. In the absence of Zn2+, the relaxivity of the complex was 5.0 ± 0.1 mM−1s−1 (37°C, pH 7.6 0.1M Tris buffer, 23 MHz) and this gradually increased to 6.0 ± 0.1 mM−1s−1 with addition of Zn2+ until two equivalents had been added (Figure 1). This shows that a 1:2 (Gd:Zn) complex is formed. These r1 values are similar in magnitude to other GdDOTA-bis-amide complexes previously reported.23 The relaxivity of GdDOTA-diBPEN remained constant when dissolved in 0.1 M phosphate buffer over a period of 24 hours, indicating that the agent is stable in a biological medium.24 The relaxivity of GdDOTA-diBPEN did not change upon addition of Ca2+ or Mg2+ but did increase to 6.3 ± 0.1 mM−1s−1 (37°C, pH 7.6 tris buffer, 23 MHz) with addition of two equivalents of Cu2+. Like Zn2+, the relaxivity did not increase beyond this value with further addition of Cu2+. Given that GdDOTA-bis-amide complexes are known to display slower water exchange rates than required for optimal relaxivity, the small increases in relaxivity promoted by binding of either Zn2+ or Cu2+ likely reflect an increase in water and/or proton exchange rates, perhaps catalyzed by Zn2+-bound hydroxy groups as previously found for the PARACEST-based Zn2+ sensor, EuDOTA-bisBPEN-di-butyl tetraamide21 or by creating a more organized second sphere of water molecules (those bound to Zn2+ or Cu2+) in close proximity to the single Gd3+-bound water molecule of the complex. The mechanistic differences that determine the relaxivity change in response to Zn2+ for GdDOTA-diBPEN (altering water or proton exchange) as compared to the DTPA derivative reported by Nagano and coworkers17 (Gd3+ water coordination blockage when two BPEN units coordinate one Zn2+ above the Gd3+) might be explained by the difference in charges between these two Gd3+ complexes. In the former, the complex has a net positive charge while the later Gd3+ complex is neutral so that a 1:1 Zn2+ complex formation involving both BPEN units placing Zn2+ above the Gd3+ is less electrostatically restricted compared to GdDOTA-diBPEN. MALDI-TOF spectra of the GdDOTA-diBPEN-Zn2+ adduct showed two major peaks, m/z 1208 and 1108 assigned to GdDOTA-Zn2(OH)4diBPEN and GdDOTA-Zn(OH)2diBPEN, respectively (See supporting information Fig.S1). The high resolution 1H NMR spectrum of EuDOTA-diBPEN shows that both possible coordination isomers are present in solution, the square antiprismatic (SAP) and twisted square antiprismatic (TSAP) in an approximate 3:2 ratio (See supporting information Figure S2) and that this ratio does not change with addition of Zn2+.

Figure 1
Relaxivity of GdDOTA-diBPEN at 23 MHz and 37°C in the presence of MCl2, where M (=) Zn2+, Cu2+, Ca2+, or Mg2+. All solutions were prepared in 100 mM tris buffer at pH 7.6.

The apparent binding dissociation constant (KD) of GdDOTA-Zn2diBPEN was determined by a competitive assay using the commercially available fluorophore ZnAF-2F (See supporting information and Figure S3). This dye was chosen since it shares similar Zn2+ binding moieties as GdDOTA-diBPEN and binds the metal with high affinity (KD= 5.5 nM).25 Using the published dissociation constant for the dye, a binding constant of 33.6 ± 0.4 nM was estimated for formation of GdDOTA-Zn2diBPEN (per BPEN binding unit). As a reference, a similar ligand, bis(6-pyridinylmethyl)amine has been reported to form 1:1 complexes with Zn2+ with a KD of 26.9 nM.26

Given that GdDOTA-diBPEN has two aromatic rings per BPEN unit, a further study was performed to investigate possible interactions between the agent and human serum albumin (HSA), the most abundant protein in blood and cerebral spinal fluid. Since metal ion mediated binding of small molecules to albumin has been reported previously,27 we also examined the influence of Zn2+ on binding interactions between the agent and albumin. A titration experiment performed by measuring the relaxation rate of a solution of GdDOTA-diBPEN upon addition of fatty acid-free HSA showed that the agent alone binds only weakly with HSA possibly due to non-specific electrostatic interactions. Surprisingly, when 2 equivalents of Zn2+ were added to the agent prior to addition of HSA, a quite different result was obtained (Figure 3). In this case, binding of the agent to HSA was surprisingly strong and the relaxivity of GdDOTA-diBPEN increased from 6.6 ± 0.1 mM−1s−1 to 17.4 ± 0.5 mM−1s−1 when fully bound to the protein (Figure 2), a 165% increase. A similar increase in relaxivity was observed for the GdDOTA-Cu2diBPEN upon addition of HSA but no change was observed for the complex containing either Ca2+ or Mg2+. The increase in relaxivity observed for GdDOTA-Zn2diBPEN when bound to HSA likely reflects a slower rotational correlation time (τR) for the complex when bound to the protein.

Figure 2
Relaxivity of GdDOTA-diBPEN at 23 MHz and 37° C in the presence of MCl2, where M (=) Zn2+, Cu2+, Ca2+, or Mg2+. All solutions were prepared in 100 mM tris buffer at pH 7.6 plus 600 μM human serum albumin (HSA).
Figure 3
Titration of GdDOTA-diBPEN 1 mM (triangles), and GdDOTA-diBPEN 1 mM plus 2 mM of Zn2+ (squares) with HSA. All measurements were made at 23 MHz and 37° C in 100 mM tris buffer at pH 7.6. The solid curves represent the best fit to equation 3 in ...

To determine the KD of GdDOTA-Zn2diBPEN with HSA, the proton relaxation enhancement, ε* (equation 2 in supporting information) versus HSA concentration was then fitted to a single site-specific binding model28 (equation 3 in supplementary information). The best fit to the model gave a KD of 42 ± 9 μM for GdDOTA-Zn2diBPEN binding to HSA.

Fluorescent displacement experiments were performed using warfarin and dansylglycine as probes to determine whether GdDOTA-Zn2diBPEN binds at site 1 or 2 of subdomain IIA or IIIA of HSA.29 As shown in Figure 4, there is a decrease in dansylglycine fluorescence when increasing amounts of GdDOTA-Zn2diBPEN are added which suggests that the complex binds HSA in site 2 of subdomain IIIA. The KD for GdDOTA-Zn2diBPEN to HSA was determined from these displacement results by assuming one site binding (equations 4 and 5 in supplementary information).30 The fitting of the data gave a KD of 48 ± 6 μM, a value similar to that described above in the proton enhancement titration experiment. Similar experiments were performed using GdDOTA-diBPEN alone (no divalent metal ion) but no displacement of either fluorescent probe was observed.

Figure 4
Displacement fluorescence plots for warfarin (squares, 5μM) and dansylglycine (circles, 5μM) from equimolar HSA by GdDOTA-Zn2diBPEN at 25°C in 100 mM tris buffer at pH 7.6. The solid curves represent the best fit to the data.

Although non-metal ion mediated, previous reports of positively charged GdDOTA complexes that bind HSA in site 2 can be found in the literature but no definitive binding model has been provided.31 To provide a model of how GdDOTA-Zn2diBPEN might bind to HSA, we first performed a structural analysis of site 2 using literature data. Site 2 comprises a largely pre-formed hydrophobic cavity (~16 Å deep and ~8 Å wide with a radius curvature ~8.5 Å) with distinct polar features. It contains a cationic group located close to one side of the entrance of the binding pocket (R410) but also contains S489, E382 and N386 located near the surface on the opposite side. Y411 is centered inside the cavity (as a reference site 1 contains a cluster of four cationic residues at the entrance of the cavity K195, K199, R218, R222). Unlike binding site 1 where the binding entrance is enclosed by subdomains IIB and IIIA, the entrance to site 2 is not encumbered and is exposed to solvent. It is also known that binding to this site is favored with compounds that are planar or near planar and that contain aromatic rings.32 The fact that GdDOTA-diBPEN interacts only weakly with HSA, while Zn2+ enhances this binding interaction considerably indicates that Zn2+ complexation “locks” the two aromatic pyridine rings, making the ZnBPEN unit better configured to fit into the binding site. Presumably the agent might first interact with S489, E382 and N386 at one side of the entrance given its positive net charge and once inside the cavity, the hydroxyl group of Y411 may interact with the Zn2+ and thereby provide further binding stability (see supporting information Figure S4 for a molecular model depicting the binding of GdDOTA-Zn2diBPEN to HSA). With this model, it is reasonable to consider that the presence of R410 on the opposite side of the cavity restricts rotational freedom of the bound agent through the methylene units connecting BPEN to the GdDOTA moiety. This could explain the dramatic increase in relaxivity that is observed for the agent bound to HSA, similar to that previously reported for positively charged GdDOTA type complexes.31

In vitro MR images of GdDOTA-diBPEN at 9.4-T in the presence of various Zn2+ concentrations demonstrates that Zn2+ can be detected at concentrations as low as 30 μM (Figure 5). Unlike Zn2+, there were no discernible differences in image intensity for samples of GdDOTA-diBPEN with added Ca2+ or Mg2+ (not shown).

Figure 5
T1-weighted phantom MR images. Images of a 100 μM solution of GdDOTA-diBPEN in Tris buffer with 600 μM HSA at various concentrations of ZnCl2. Spot A, 200 μM Zn; B, 100 μM Zn; C, 30 μM Zn; D, 0 μM Zn; E, ...

To determine the effectiveness of GdDOTA-diBPEN as a relaxation agent under physiological conditions, relaxivity measurements were also performed in human blood serum. In this case, the relaxivity increase upon addition of Zn2+ was smaller, from 6.1 ± 0.1 mM−1s−1 to 8.6 ± 0.1 mM−1s−1 (37°C, pH 7.6 Tris buffer, 23 MHz) than observed previously for the agent in the presence of fatty acid free HSA. Nevertheless, this 40% increase in relaxivity should still be enough to be detectable by MRI in vivo upon release of free Zn2+.

Based upon the binding equilibrium constants between GdDOTA-diBPEN, Zn2+ and HSA (multiple equilibria) and the relaxivities of GdDOTA-diBPEN, GdDOTA-Zn2diBPEN and ternary GdDOTA-Zn2diBPEN-HSA, one should be able to make a reasonable prediction of the detection limits of the Zn2+ sensor system in vivo. The minimum requirement is that the agent should be able to sense free, chelatable Zn2+ released in response to a particular metabolic event or metabolic abnormality. The CA should have a relatively strong binding affinity for Zn2+ but not so strong that it interferes with other endogenous metabolic processes. Given that HSA (a main component in blood) has a binding affinity for Zn2+ of 29.5 nM33 and if one assumes that the local extracellular concentration of free chelatable Zn2+ near excitable cells rises to at least 475 μM (as predicted for pancreatic β-cells),9 then one can project the ideal tissue concentration of the Zn2+ sensor required to detect such an increase. If one first assumes that a standard clinical dose of CA will be injected (0.1 mmol/kg corresponds to an average extracellular tissue concentration of ~500 μM) and that [HSA] in blood is ~600 μM, then a multiple equilibrium model (see supporting information) using the binding constants reported here predicts a steady-state concentration of ternary GdDOTA-Zn2diBPEN-HSA complex of ~131 μM with 27% of the total agent bound to Zn2+. Given the relaxivity values of the various GdL species in human blood serum, this would correspond to a relaxation rate change (ΔR1) of 0.31 s−1 (~10%) between a high Zn2+ situation (475 μM) and normal physiology (~20 μM Zn2+ in blood).34 This change in relaxation rate would be easily detected in an image. Ideally, it would be preferable to use a much lower concentration of sensor at a level where the agent is essentially silent (no “background signal”) under normal physiological Zn2+ levels but then increases upon Zn2+ release from tissues. The lowest concentration of agent required to detect 475 μM of Zn2+ can be determined by extrapolating the previous calculation to a ΔR1 of 0.07 s−1, which is the predicted detection minimum of a targeted GdL sensor reported recently.35 The calculated value corresponds to 50 μM GdDOTA-diBPEN (Figure 6A) which coincides with the actual “silent” sensor condition of 50 μM GdDOTA-diBPEN in human blood serum. So, given the relaxivity and binding characteristics of the Zn2+ sensor reported here, one would anticipate being able to inject only minimal amounts of sensor (50 μM) and still detect increases in Zn2+ concentrations approaching those expected for excitable cells. A second question that can be addressed using this same model is how important is the binding affinity between the sensor and Zn2+ for it to be useful in vivo? Figure 6B illustrates the estimated fractional amount of sensor-Zn2+ complex formed with 500 μM sensor and the indicated amount of total Zn2+ for four different binding constants. If one again uses a ΔR1 of 0.07 s−1 as the lower limit of detection (this corresponds to ~6% of the total agent bound to Zn2+), then one predicts that a sensor with a KDGdLZn = 1 μM would only detect changes in Zn2+ concentration approaching 500 μM while a sensor with a KDGdLZn = 33.6 nM (the agent reported herein) should detect changes in Zn2+ as low as ~100 μM. This highlights the importance of the Zn2+ binding constant in the in vivo study.

Figure 6
A. Calculated plots of ΔR1 versus GdDOTAdiBPEN concentration for a 475 μM Zn2+ rise from physiological conditions at various dissociation constants of the agent to Zn2+. [HSA] = 600 μM and relaxivity values were used as determined ...

Conclusions

A novel GdDOTA-based Zn2+ sensor is reported that displays only a modest relaxivity increase upon binding to two equivalents of Zn2+ but shows a substantially larger increase in relaxivity (165%) when the ternary GdL-Zn2+ complex is bound to HSA. The Zn2+ sensor does not respond to either Ca2+ or Mg2+ but does show a similar relaxation enhancement in the presence of Cu2+. This competition however would likely not interfere in vivo because Cu2+ is typically found at much lower concentrations in tissues compared to Zn2+. In vitro imaging studies indicate that the present agent can detect Zn2+ at concentrations as low as ~30 μM in presence of HSA. Further analysis shows that the binding dissociation constant of any CA to Zn2+ with relaxivities in serum close to the agent presented herein should be at least 1 μM if the goal is to apply such systems in vivo.

Supplementary Material

1_si_001

Acknowledgements

The completion of this work was possible due to the support of CONACYT, Mexico sabbatical fellowship 75381 and by NIH grant DK-058398.

Footnotes

Supporting Information Available.

General experimental details; full experimental details including synthetic and characterization data for compound 3, and its Gd3+ and Eu3+ complexes; full experimental details for fluorescence displacement experiments, relaxivity measurements, data fitting, modeling programs and mathematical derivations are provided. A proposed model figure of the binding of the CA to HSA is also included. This information is available free of charge via the Internet at http://pubs.acs.org.

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