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Although calpain (EC 3.4.22) protease activation was suggested to contribute to excitotoxic delayed calcium deregulation (DCD) via proteolysis of Na+/Ca2+ exchanger 3 (NCX3), cytoplasmic calpain activation in relation to DCD has never been visualized in real time. We employed a calpain fluorescence resonance energy transfer (FRET) substrate to simultaneously image calpain activation and calcium deregulation in live cortical neurons. A calpain inhibitor-sensitive decline in FRET was observed at 39 ± 5 min after DCD occurred in neurons exposed to continuous glutamate (100 μM). Inhibition of calpain by calpeptin did not delay the onset of DCD, recovery from DCD-like reversible calcium elevations (RCE), or cell death despite inhibiting α-spectrin processing by >90%. Na+/Ca2+ exchangers reversed during glutamate exposure, the NCX antagonist KB-R7943 prolonged the time to DCD, and significant NCX3 cleavage following 90 min of glutamate exposure was not observed. Our findings suggest that robust calpain activation associated with acute glutamate toxicity occurs only after a sustained loss in calcium homeostasis. Processing of NCX3 or other calpain substrates is unlikely to be the primary cause of acute excitotoxicity in cortical neurons. However, a role for calpain as a contributing factor or in response to milder glutamate insults is not excluded.
Modes of cell death in acute and chronic neurodegenerative disorders are widely varied and brain-region selective pathology is frequently observed (Bredesen et al. 2006;Lin and Beal 2006). Despite this variability there is a pervasive view that antagonizing Ca2+-dependent calpain proteases is universally protective. Two ubiquitous isoforms, μ-calpain (EC 126.96.36.199) and m-calpain (EC 188.8.131.52), are implicated in neurodegeneration (Bevers and Neumar 2008). In vitro, these isoforms are activated by micromolar and millimolar concentrations of calcium, respectively. Although intracellular calcium in healthy neurons is generally ~100 nM and does not reach millimolar levels until complete loss of calcium homeostasis occurs (Randall and Thayer 1992;Nicholls 2004), calpain activation prior to calcium deregulation has been explained by close proximity to NMDA receptors (Hewitt et al. 1998;Adamec et al. 1998;Vanderklish et al. 2000), sodium-calcium exchangers (Araujo et al. 2007), or other routes of local calcium elevation (Friedrich 2004).
Calpain activation near glutamate receptors was reported within five minutes of NMDA receptor activation (Vanderklish et al. 2000;Lankiewicz et al. 2000). NMDA, α-amino-3-hydroxy-5-methylisoxazole-4-propionate (AMPA), and mGluR1 receptor subunits are initial targets of calpain processing (Guttmann et al. 2002;Simpkins et al. 2003;Wu et al. 2005;Xu et al. 2007;Yuen et al. 2007a;Yuen et al. 2007b). By ameliorating the extent of intracellular Ca2+ and Na+ elevation during short bursts of glutamate receptor overactivation, these initial proteolysis events may protect the neuron from “accidental excitotoxicity.” In contrast, sustained glutamate receptor activation leads to the calpain-dependent processing of numerous substrates, many with deleterious consequences for cell survival. Substrates include the cytoskeletal proteins α-spectrin and microtubule-associated protein 2 (MAP2) (Siman and Noszek 1988;Springer et al. 1997), plasma membrane calcium ATPases (PMCAs) (Pottorf et al. 2006), calcineurin phosphotase (Wu et al. 2004), the cyclin-dependent kinase 5 activator p35 (Lee et al. 2000), and apoptosis-inducing factor (AIF) (Polster et al. 2005;Cao et al. 2007).
The point-of-no-return for a neuron succumbing to excitotoxic injury is generally considered the time at which intracellular calcium homeostasis is irreversibly lost, i.e. delayed calcium deregulation (DCD) (Nicholls 2004). Events that contribute to the onset of DCD are predicted to influence cell survival with an opportunity for therapeutic intervention. However, events that occur after DCD are predicted to influence the timing of cell death without altering its inevitability. Calpain processing of sodium-calcium exchanger (NCX) isoform 3 and loss of calcium extrusion capacity was directly implicated in the delayed calcium rise observed in glutamate-treated cerebellar granule neurons (Bano et al. 2005). However, the relative expression of cleavage-resistant NCX1 to NCX3 is higher in forebrain neurons as compared to cerebellar granule neurons (Kiedrowski et al. 2004) although granule neurons are more resistant to DCD (Brorson et al. 1995;Castilho et al. 1998;Stout et al. 1998;Vergun et al. 1999;Bano et al. 2005;Bolshakov et al. 2008). This raises the possibility that alternate mechanisms precipitate DCD in forebrain neurons before calpain-mediated loss of functional NCX3 limits calcium homeostasis.
In this study we tested the hypotheses that: 1) DCD can occur without calpain activation in primary cortical neurons exposed to an excitotoxic concentration of glutamate, and 2) calpain activation converts DCD from a reversible to an irreversible event. This was accomplished by conducting simultaneous live cell imaging of calpain activation and intracellular calcium deregulation, investigating the function and processing of NCX, and testing the efficacy of calpain inhibitors against DCD, DCD-like reversible calcium elevations (RCE), and cell death. Our results define major cytoplasmic calpain activation in forebrain neurons as a distinct event occurring downstream of DCD that is not required for acute glutamate toxicity.
Tetramethylrhodamine methyl ester (TMRM+), Fura-4F-AM, Fura-6F-AM, Fluo-4FFAM, SBFI-AM, lipofectamine 2000, Neurobasal medium, B27 supplement, and GlutaMAX were from Invitrogen (Carlsbad, CA). Ionomycin, calpeptin, and PD150606 were purchased from EMD Biosciences (San Diego, CA). Primary rabbit polyclonal antibody to NCX3 was a kind gift of Dr. Ken Philipson (UCLA, Los Angelos, CA). Mouse monoclonal antibody to β-actin (clone AC-74) was from Sigma (St. Louis, MO). Mouse monoclonal antibody to α-spectrin (MAB1622) was from Chemicon (Temecula, CA). The pYSCS plasmid was generously provided by Dr. Peter Vanderklish (The Scripps Research Institute, San Diego, CA). All other reagents were purchased from Sigma unless otherwise indicated.
Primary cortical neurons were prepared from 1-2 pairs of E18 rat cortices (BrainBits™, LLC, Springfield, IL) by papain dissociation followed by gentle trituration and used at 11-14 days in vitro (DIV). Briefly, cortices were washed in 2 ml of Hibernate™ medium (BrainBits™) and then digested with 2 mg/ml papain (Worthington) in Hibernate™ for 30 min at 37° C. Tissue was dispersed manually by 5-10 strokes with a 1 ml pipette and cells were plated onto poly-D-lysine-coated Lab-Tek 8-well chambered coverglasses (Nunc) at a density of 1 × 105cells/well. Cells were initially plated in Neurobasal medium containing B27 supplement (2%), L-glutamine (0.5 mM), fetal bovine serum (1%), penicillin (100 IU/ml) and streptomycin (100 μg/ml). For the majority of experiments, neurons were maintained at 37°C in an oxygen-regulated incubator with a humidified atmosphere of 92% N2/5% CO2/3% O2. Every 3-4 days after the initial plating half of the medium was replaced with fresh medium lacking serum and containing GlutaMAX (0.5 mM) in place of L-glutamine. When stated, results with neurons cultured at physiologically relevant 3% O2 (Grote et al. 1996) were confirmed using neurons cultured in a conventional 95% air/5% CO2 environment. All experiments were conducted in room air.
For simultaneous assay of DCD and calpain activation, cortical cultures were transfected with the pYSCS FRET sensor (Vanderklish et al. 2000) on day 9 using Lipofectamine 2000 (Invitrogen) in Neurobasal medium at a 3:2 ratio of Lipofectamine (αl) to DNA (αg); 0.2αg of DNA was transfected per well in 8-well Lab-Tek chambers. Experiments were carried out at 3 days post-transfection (at 12 DIV). The pYSCS transfected cultures were incubated with Fura-6F-AM (3 αM; Molecular Probes / Invitrogen) for 25 min at 37°C in imaging medium containing (in mM): 120 NaCl, 3.5 KCl, 1.3 CaCl2, 0.4 KH2PO4, 20 N-Tris-(hydroxymethyl)-methyl-2-amino-ethanesulphonic acid (TES), 5 NaHCO3, 1.2 Na2SO4, 15 D-glucose, the pH was set to 7.4 by NaOH (considering a pKa of 7.5 for TES, by 8.9 mM NaOH, yielding [Na+]e=136mM). For dye loading or in experiments with superfusion the imaging medium was supplemented with MgCl2 (1 mM).
To image initial glutamate-triggered ionic changes, untransfected cortical cultures were incubated with Fura-4F-AM (1 μM; Molecular Probes / Invitrogen) for 20 min or with SBFI-AM (6 μM; in the presence of 0.02% Pluronic F127) for 40 min at 37°C in Mg2+-supplemented imaging medium and subsequently rinsed. To monitor plasma membrane potential (ΔΨP), the plasma membrane potential indicator (PMPI; Molecular Devices #R8042 FLIPR Membrane Potential Assay Kit) was used at 1:200 dilution of the Loading Buffer (specified by the manufacturer).
Imaging was performed on an Olympus IX-81 inverted microscope equipped with a UAPO 20x air 0.75NA lens, a Lambda LS Xe-arc light source (175W), Lambda 10-2 excitation and emission filter wheels (Sutter Instruments, Novato, CA), a ProScan linear-encoded xy-stage (Prior, Rockland, MA) and a Coolsnap HQ cooled digital CCD camera (Photometrics, Tucson, AZ). The filter sets given as excitation-dichroic mirror-emission (in nm) were: for YFP or PMPI 500/24 (band pass) - 520LP (long pass) - 542/27 (Semrock, Rochester, NY), for CFP 438/24 - 458LP - 483/32 (Semrock), for FRET 438/24 - 458LP - 542/27 (Semrock) and for Fura dyes and SBFI 340 or 380 (Chroma, Rockingham, VT) - 458LP - 420LP. The whole microscope was maintained at 37°C in air using an environmental enclosure.
Image acquisition was controlled by the Multi-Dimensional Acquisition application in Metamorph 6.3 (Molecular Devices). Images were recorded by using an ND1.0 (neutral density) attenuator, 100-200 ms exposure times and 3×3 binning in 2 minute intervals in 12 view fields at the following 5 wavelengths (defined above): YFP, CFP, FRET, Fura 340 and 380 nm. In each acquisition cycle the sample was auto-focused on green fluorescent beads placed in an empty well of the 8-well chamber. Fluorescence intensities were determined over the soma of pYSCS-expressing neurons after background subtraction. The background was defined by the peak of the intensity histogram of each frame. All of the following calculations were done by the region of interest (ROI) average intensities, except for images and videos shown, where calculations were performed pixel-by-pixel.
The FRET probe pYSCS responds to the proteolysis of its α-spectrin calpain cleavage site with an irreversible loss of FRET (Vanderklish et al. 2000). Although a portion of the encoded protein localized to post-synaptic densities, the majority of the fluorescence was in the cytoplasm. We found that the fluorescence of the eYFP component of pYSCS exhibited a substantial decrease in intensity when neurons were stimulated with glutamate or underwent DCD. This observation is consistent with quenching of the eYFP FRET acceptor by a decrease in pH or an increase of [Cl-] in glutamate-stimulated neurons (Wu et al. 1999;Zhang et al. 2002;Slemmer et al. 2004). In addition to the cross talk between CFP, YFP and FRET channels, fluorescence of Fura-6F significantly contributed to the CFP and FRET images and the CFP and the YFP to the Fura-6F emission in brightly expressing cells. Therefore the measured fluorescence intensities (F) were first spectrally (linearly) unmixed (resulting fluorescence intensities specific for each fluorophore F', see Supplementary Methods). [Ca2+]c is given as the ratio of unmixed Fura-6F intensities (). Because of saturating concentrations of intracellular calcium after DCD RFura6F was not calibrated to show micromolarity. To correct for the loss of eYFP fluorescence yield, a three wavelength-corrected FRET ratio calculation was used in place of a simple emission ratio that was used earlier in fixed cells, where ionic effects did not contribute (Vanderklish et al. 2000). This was expressed as FRET = . Image analysis was performed in custom software developed in Delphi 2009 (Embarcadero Technologies, San Francisco, CA).
The kinetics of corrected FRET ratio and Fura-6F ratio were heterogeneous among neurons. To provide a relative comparison of changes in these signals, temporal cross-correlation of differentiated signals was calculated. Because cross-correlalograms show the relative relationship of compared signals in time, the cell-by-cell heterogeneity cancels and the mean of the correlalograms can be calculated. Calculations were performed using standard functions of Mathematica 5.2 (Wolfram Research, Champaign, IL), see Supplementary Methods. Cross-correlalograms provide the following information when `trace 1' (FRET) is compared to `trace 2' (Fura-6F): changes in trace 1 and 2 occurring at the same time in the same direction (increase or decrease) are indicated by a positive peak at zero time. Changes occurring at the same time in the opposite direction (for instance decrease in 1 and increase in 2) are indicated by a negative peak. When the change in 1 occurs after the change in 2, the peak of the cross-correlation diagram shifts to a positive time lag.
Wide-field image acquisition was performed as stated above, but by Metafluor 6.3 (Molecular Devices). For imaging Fura-4F, an ND 1.5 attenuator, 4×4 binning, and 400 and 200 ms exposure times were used for the 340 and 380 nm excitations, respectively, at a four seconds image acquisition interval. For imaging SBFI plus PMPI, an ND 0.5 attenuator, 4×4 binning, and 300, 100 and 30 ms exposure times were used for SBFI 340, 380 nm and PMPI 500 nm excitations, respectively. There was no measurable cross-talk between SBFI and PMPI emissions.
Fura-4F ratios (R=F340/F380) were calibrated to micromolarity using the [Ca2+]=Kd×((R-Rmin)/(Rmax-R))×Ffree380/Fsat380 equation (Grynkiewicz et al. 1985). Cells were exposed to zero and saturating (10 mM free) [Ca2+], yielding the minimal fluorescence ratio (Rmin) plus the intensity of the Ca2+-unbound Fura-4F at the 380 nm excitation (Ffree380) and the maximal ratio (Rmax) plus the intensity of Ca2+-saturated Fura-4F at the 380 nm excitation (Fsat380), respectively. To calculate the Ffree380/Fsat380 ratio, cell swelling and dye leakage were compensated by normalization of Ffree380 and Fsat380 with the isosbestic fluorescence intensity calculated for the matching time points (Gerencser and Nicholls 2008). To obtain the above parameters, the imaging medium was replaced with `intracellular medium' containing (in mM): 123 KCl, 12.4 NaCl, 20 KOH, 10 EGTA, 20 PIPES with, and then, without 20 mM CaCl2 (pH 7.2) and supplemented with 20 μM 4Br-A23187 (AG Scientific, San Diego), 10 μM nigericin, 10 μM monensin, and 1 μM antimycin A to establish complete equilibration of [Ca2+] and pH (Gerencser and Adam-Vizi 2005). The Kd of Fura-4F was taken as 770 nM according to the manufacturer. SBFI was calibrated similarly to Fura-4F, but parameters were obtained by a three-point calibration at rest (where [Na+]c was 11.4±0.3 mM; n=3; determined in separate experiments), after complete equilibration with the imaging medium ([Na+]e =136 mM) in the presence of gramicidin (10 μg/ml), and after wash with nominally Na+-free (choline chloride substituted) medium. This calibration provided Rmin, Rmax and Kd×Ffree380/Fsat380 from ratios measured at the above conditions (see Supplementary Methods). The PMPI fluorescence was converted to millivolts by a dynamic, Nernstian, compartment model-based calibration technique (see Supplementary Methods). The calibration was based on the measurement of PMPI fluorescence at K+-equilibrium potential (-80.4 mV) in the presence of valinomycin (1 μM; a K+-selective ionophore) and oligomycin (2 μg/ml) (at [K+]e=3.9 mM and [K+]c=79.1±1.4 mM; n=3), and at zero ΔΨP after addition of gramicidin (10 μg/ml; a K+-Na+-ionophore). The resting ΔΨP was -59.9±1.2 mV (n=3 exps.). For the glutamate-treatment experiments the resting and zero potentials were used as calibration points, and the gramicidin treatment was common calibration step with the [Na+]=136 mM point of the SBFI calibration. This procedure was performed at the end of each experiment and calculations were done cell-by-cell in Mathematica 5.2. The means ± SE of the calibrated values are shown.
To calculate ΔG, the measured mean [Ca2+] , [Na+]c and ΔΨP in addition to [Na+]e=136 mM and [Ca2+]e=1.3 mM were used:
In Eq. 1 R=8.3 J mol-1 K-1 (gas constant); T=310 K (temperature); F=96485 J mol-1 V-1 (Faraday constant), n is the exchange ratio and the ΔΨP is given in negative volts. The exchange ratio of Na+:Ca2+ is known to be near to 3:1, but higher, non-integer values were also often reported (Blaustein and Lederer 1999;Bers and Ginsburg 2007). In resting neurons the NCX is considered to be in equilibrium, thus its ΔG is zero. In our case this was satisfied with n=3.1, therefore this value was used to calculate ΔG. The error of ΔG was calculated by propagating the SE of the measured parameters though the calculations (Gerencser et al. 2008).
Imaging was performed using an LSM 5 Pascal laser scanning confocal system (Carl Zeiss AG, Oberkochen, Germany) with a 20x NEOFLUAR NA0.5 air objective lens in room air in a temperature-controlled enclosure at 37°C. For simultaneous imaging of DCD and membrane potential changes, neurons in 8-well Lab-Tek chambers were loaded with Fluo-4FF-AM (0.5 μM) and TMRM+ (5 nM) in the presence of tetraphenylboron (1 μM) in imaging medium for 60-90 minutes at 37°C. Fluo-4FF and TMRM+ were simultaneously excited at 488 and 543 nm and emissions were monitored using a 505-530 nm and a 560LP filter, respectively. There was no measurable cross-talk between the emissions of Fluo-4FF and TMRM+.
Fluorescent time-courses from four independent wells of the Lab-Tek dish were acquired in parallel using the Multi Time Lapse Module, recording a time point for each well at every 4 min. Neurons displaying TMRM+ fluorescence that was lost upon glutamate stimulation (signifying ΔΨP depolarization) were selected for analysis.
For cell viability experiments, membrane potential polarization, apoptosis, and cell death were determined in parallel using TMRM+, the early plasma membrane permeabilization marker Yo-Pro-1, and the necrosis marker propidium iodide (PI), respectively. The dyes were simultaneously excited at 488 and 543 nm, and emissions were detected at 505-530 nm for Yo-Pro-1, at 560-615 nm for TMRM+ and at 608-683 nm for PI. The PI staining was punctate and distinctly nuclear while TMRM+ fluorescence was cytoplasmic, enabling us to use these dyes in concert despite partial spectral overlap. Neurons pre-treated with 10 μM calpeptin or vehicle for 30 min in imaging buffer were subsequently exposed to glutamate. After 30 min of glutamate or vehicle treatment, imaging buffer was removed and replaced with previously saved culture medium diluted with fresh medium at 1:1. This medium was supplemented with HEPES (20 mM) to buffer pH and MK-801 (10 μM) and 2,3-dihydroxy-6-nitro-7-sulfamoylbenzo[f]quinoxaline-2,3-dione (NBQX; 10 μM) to block NMDA and AMPA receptors, respectively. The number of healthy neurons defined as TMRM+ positive, PI negative, the number of apoptotic neurons defined as Yo-Pro-1 positive, PI negative, and the number of necrotic neurons defined by PI positive nuclear staining were counted at 0, 4, 8, 12, and 20 hours following glutamate treatment.
Following the acquisition of fluorescent time-courses, imaging buffer was aspirated from the wells and neurons were lysed by scraping in 50 μl of radioimmunoprecipation assay buffer consisting of 150 mM NaCl, 50 mM Tris, 1 mM EDTA, 1 mM EGTA, 1% Triton X-100, 0.5% sodium deoxycholate, 0.1% sodium dodecylsulfate, and 1× Halt Protease Inhibitor Cocktail (Pierce Biotechnology, Rockford, IL) or Protease Inhibitor Cocktail Set III (EMD Biosciences), pH 7.4. Solubilization of membrane proteins was enhanced by three freeze-thaw cycles followed by incubation in SDS-PAGE sample buffer containing 5% β-mercaptoethanol for 30 min at 37° C. Seven to 20 μg of neuronal protein was separated on 4-20% Tris-HEPES gradient gels (ISC BioExpress, Kaysville, UT) by SDS-PAGE and transferred to PVDF membranes (Bio-Rad, Hercules, CA). Immunodetection was performed by standard procedures using SuperSignal West Pico or West Fempto enhanced chemiluminescence substrates (Pierce Biotechnology) and the following dilutions of primary antibodies: 1:500 anti-α-spectrin (0.2 μg/ml), 1:5000 anti-β-actin, 1:1000 anti-NCX3.
The incidence and time to DCD in the absence and presence of calpain inhibitor was analyzed for statistical differences using paired Student's t-test comparing the same-day controls. Numbers of neurons exhibiting RCE or recovery were compared with Fisher's exact test in GraphPad Prism 4 (GraphPad Software, Inc., San Diego, CA). To examine whether calpeptin modified the effect of glutamate on TMRM+ or propidium iodide fluorescence, logistic regression analysis was used (see Supplemental Methods). Averages are represented in the text as mean ± SE.
Cytoplasmic calcium changes and calpain activity were monitored in single cells simultaneously with wide field microscopy using the low affinity Ca2+ dye Fura-6F (Fig. 1a) and the FRET-based, genetically encoded calpain sensor pYSCS (Fig. 1b), respectively. Cleavage of the pYSCS-encoded protein within the α-spectrin sequence targeted by calpain is expected to trigger an irreversible loss of the FRET between the CFP FRET donor and the YFP FRET acceptor (Vanderklish et al. 2000). Cortical cultures were pre-treated with calpeptin (10 μM) or vehicle (DMSO 1:2000) for 30 min and then exposed to glutamate (100 μM; with 10 μM glycine in the absence of Mg2+). Calpeptin (or vehicle) and glutamate were present for the duration of the experiment.
Glutamate evoked a small initial increase of intracellular calcium, as monitored by the Fura-6F ratio over the soma (Fig. 1c-d, black trace). This small calcium increase was followed by delayed calcium deregulation at 5-30 minutes (red pseudocolor in Fig. 1aii and iii). The initial response of the calpain sensor to glutamate was a 14.1 ± 0.8% drop in the amount of FRET in the soma (compared to the baseline; n=58, Fig. 1c, red trace) that was not inhibited by calpeptin (15.2 ± 0.9% n=59). Moreover, in the presence of calpeptin this initial decrease in FRET spontaneously reversed, excluding the possibility that this drop was due to irreversible cleavage of the sensor by calpain (Fig. 1d, red trace; bii). Shortly before DCD the FRET exhibited another calpeptin-insensitive and reversible decrease (Fig. 1c,d, open arrows; see also analysis below). The only calpeptin-sensitive, irreversible, `major drop of FRET' (to 37 ± 3% below baseline; n=23) likely to reflect calpain activation occurred long after DCD (Fig. 1c red arrow; Biii, cells b and c). Forty-four of the 58 vehicle pre-treated cells underwent DCD (at 20 ± 3 min after addition of glutamate), and 23 of these also exhibited `calpain activation' typical to that denoted by the red arrow in Fig. 1c. No such change in FRET occurred in cells that did not undergo DCD. We observed only 2 cells with similar FRET characteristics in 49 calpeptin pre-treated neurons undergoing DCD.
Pairs of boxes in Fig. 1e depict the time to DCD and the time to `calpain activation' (relative to glutamate addition) for individual neurons. Pairs connected by lines with a positive (upward) slope indicate neurons exhibiting `calpain activation' subsequent to DCD. Of note, `calpain activation' was objectively defined as the steepest decrease in FRET beyond a threshold using a computer algorithm. In a few cells (negatively sloped lines in Fig. 1e) the small second drop (Fig. 1c,d, open arrows) was categorized as `calpain activation' by the criterion of steepest decrease. Cells that exhibited DCD but no `calpain activation' during the measured time-frame are shown by triangles. The mean lag time between DCD and calpain activation was 39 ± 5 min (n=23).
The relationship between DCD (increase of Fura-6F ratio) and calpain activation (decrease of pYSCS FRET) was also quantified by calculation of temporal cross-correlation of differentiated signals (Fig. 1f,g). The only marked change in the Fura-6F ratio in the 5-120 min time window after glutamate addition was the increase corresponding to DCD. In contrast the FRET signal typically exhibited multiple drops. In the cross-correlalograms Fig. 1f,g, negative peaks mark changes in the two signals in opposite directions (see Methods). Both control and calpeptin treated cross-correlalograms (Fig. 1f,g) indicate a drop of the FRET signal shortly before DCD (open arrows) that corresponds to the reversible, calpeptin-insensitive decrease in FRET. Importantly, the mean cross-correlalogram in vehicle control-treated neurons (Fig. 1f, solid arrow) has a second negative peak at +39 min that is not present in calpeptin pre-treated cells (Fig. 1g). This calpeptin-sensitive negative peak indicates calpain activation average 39 minutes after DCD, confirming the `binary' calculations of Fig. 1e. The incidence and onset of DCD in pYSCS-transfected neurons was not significantly different in calpeptin-treated cultures; 49 of 59 neurons pre-treated with calpeptin underwent DCD (at 15 ± 3 min, n=49) compared to 44 of 58 (at 20 ± 3 min, n=44) pre-treated with the vehicle control.
A possible spatial heterogeneity of glutamate-triggered calpain activation was also investigated in FRET images (Fig. 1bi-iii). Detectable calpain activation (defined as the `major drop of FRET') occurred after dendrites formed injury-related varicosities (Fig. 1bii), and happened simultaneously in the soma and along the dendrites (e.g. Fig. 1biii and Supplementary Movie 1). However, the possibility of an earlier, local activation of calpain prior to DCD cannot be fully excluded by using pYSCS, because of the large calpeptin-insensitive changes as well as the small true FRET signal compared to the noise amplified by the spectral and FRET corrections.
Our imaging experiments were conducted with neurons cultured for 11-14 DIV at 3% O2, physiologically relevant to the average 2-4% O2 (15-30 mM Torr) that was measured in the rat cortex (Grote et al. 1996). Because much of the literature on glutamate excitotoxicity investigates neurons cultured at 95% air/5% CO2 (~20% O2), we repeated the Fura-6F pYSCS imaging experiments on cortical cultures grown in parallel at 20% O2. A slightly higher incidence of DCD during the measurement period was observed in neurons grown at 20% O2 (37 deregulating neurons of 39 total cells) compared to those grown at 3% O2 (44 of 58). However the properties of calpain activation were almost identical, with 18 of the 37 deregulating neurons showing calpain activation with a 46 ± 4 min delay. None of the 20% O2-cultured neurons pre-treated with calpeptin displayed the secondary loss of FRET that we associated with calpain activation.
To exclude the possibility that the calpain FRET sensor either influenced the onset of DCD or occluded an effect of calpeptin on DCD onset, we also quantified DCD in the untransfected cell population for the same experiments. As with pYSCS-transfected neurons, calpeptin did not delay the onset of DCD. In contrast, after analyzing a large number of cells we detected a slight but significant increase in the incidence of DCD following 60 minutes of glutamate exposure in calpeptin-treated neurons (Table 1). Similar results were obtained with neurons cultured at 20% O2.
To additionally confirm that calpeptin does not inhibit DCD, we imaged DCD using a different low affinity calcium indicator (Fluo-4FF; Kd~9.7 μM) in conjunction with the membrane potential sensitive dye TMRM+. Fluo-4FF undergoes a marked increase in fluorescence once calcium homeostasis is lost (Fig 2b, arrows) but is relatively insensitive to calcium increases (~1 μM) mediated by the NMDA receptor (Fig. 2b, arrowhead) (Vesce et al. 2004). Prior to glutamate addition, neurons displayed little Fluo-4FF fluorescence and a mitochondrial pattern of TMRM+ fluorescence, indicative of healthy cells with polarized plasma and mitochondrial membrane potentials (Fig 2a). Following glutamate addition, TMRM+ fluorescence was lost and neurons exhibited a stochastic and dramatic rise in Fluo-4FF fluorescence (Fig 2b and c). Under our conditions where the concentration of TMRM+ is insufficient to result in mitochondrial matrix quenching, the drop of TMRM+ signal in response to glutamate primarily reflects plasma membrane potential depolarization (Nicholls 2006) and was used to unambiguously identify neurons responding to glutamate.
Primary cortical neurons lost calcium homeostasis following 100 μM glutamate exposure with a DCD incidence and onset that was not significantly different in the absence or presence of calpeptin. 109 of 110 neurons pre-treated with calpeptin underwent DCD by 60 min (at 18 ± 4 min; n=4) compared to 106 of 107 pre-treated with the vehicle control (at 15 ± 2 min; n=4). Intracellular calcium responses in individual cells (c-d) and averaged Fluo-4FF responses (e) from a representative experiment are depicted in Fig. 2. In some experiments, the calcium ionophore ionomycin (5 μM) was added after the large Fluo-4FF increase associated with DCD. Neurons displaying high Fluo-4FF fluorescence demonstrated no further increase upon ionomycin addition, confirming that DCD had occurred in these cells.
While the calpain FRET sensor experiments suggested that calpeptin was an effective calpain inhibitor, it was important to obtain independent confirmation of intracellular calpain activation and the efficacy of calpain inhibition in our paradigm. Therefore cortical neurons incubated with glutamate for 1.5 hrs in the presence and absence of calpeptin were harvested following calcium imaging and subjected to immunoblot analysis for the canonical calpain substrate α-spectrin. Consistent with the proteolysis of the α-spectrin-based calpain FRET sensor, we observed glutamate-induced processing of α-spectrin that was inhibited by calpeptin (Fig. 3). Densiometry indicated that calpeptin inhibited the appearance of the α-spectrin breakdown product by >90%. Thus, calpeptin was an effective inhibitor of calpain activation in our experiments even though it was not an effective inhibitor of DCD.
In contrast to α-spectrin, a decrease in full-length NCX3 was not observed (Fig. 3). The 58-60 kD cleavage products reported in glutamate-treated cerebellar granule neurons (Bano et al. 2005) were barely detectable in cortical neurons relative to full-length NCX3 following 1.5 hrs of glutamate exposure (≥1 hr of deregulated calcium for most cells). It was therefore unlikely that NCX3 cleavage could account for DCD in this neuronal population.
Cortical neurons were exposed to glutamate (100 μM), plus glycine (10 μM) in the absence of Mg2+ for 12 min. This was followed by application of the NMDA and AMPA receptor antagonists MK801 (10 μM) and NBQX (10 μM), respectively, to conclude the glutamate stimulus. Nearly half of neurons maintained calcium homeostasis during and following the 12 min of glutamate receptor stimulation, i.e. no large amplitude increase in Fluo-4FF fluorescence was observed (Fig 4a). These cells recovered a mitochondrial pattern of TMRM+ fluorescence following the addition of glutamate receptor antagonists and did not undergo DCD during the subsequent 3 hrs. A small population of cells exhibited irreversible DCD (Fig. 4b). Numbers of neurons exhibiting each typical behavior are summarized in Fig. 4c.
A substantial fraction of neurons underwent a large DCD-like calcium elevation in response to glutamate that could be reversed following cessation of the stimulation (Fig. 4e-h and Supplemental Movie 3). These reversible calcium elevations (RCE) were hallmarked by maintained high [Ca2+]c during and often after glutamatergic stimulation followed by recovery of calcium homeostasis at a later time point. Recovery was either sustained (Fig. 4e,f) or followed by DCD (Fig. 4g,h). Some of the cells experienced multiple RCE events prior to DCD (Fig. 4h). TMRM+ fluorescence was restored with fast (Fig. 4e) or slow (Fig. 4f) kinetics following the recovery of calcium homeostasis. However, all neurons that failed to recover TMRM+ fluorescence following RCE later displayed DCD.
Although cytoplasmic calpain activation became measurable only after a sustained period of DCD, we hypothesized that localized calpain activity below the threshold for detection might be important for converting the DCD-like calcium elevation from a reversible to an irreversible process. Thus, we tested whether addition of calpeptin changed the frequency of RCE following transient glutamate receptor stimulation and/or improved recovery from RCE. Calpeptin (10 μM, given together with MK801 and NBQX at the conclusion of glutamate receptor stimulation) failed to affect the outcome of the glutamate treatment (Fig. 4c and d).
To support the hypothesis that an inhibitory effect of calpeptin on DCD (or RCE) was not masked by non-specific targets, we also tested the chemically unrelated calpain inhibitor PD150606 for protection against DCD. This compound interferes with the calcium binding site rather than the active site of the protease, inhibits purified μ-calpain and m-calpain in the nanomolar range (Ki = 210 nM and 370 nM, respectively), and attenuates α-spectrin breakdown at 1-10 μM in intact cells (Wang et al. 1996). As with calpeptin, 10 μM PD150606 did not decrease the incidence of DCD relative to vehicle treated cultures after 60 min of glutamate treatment although the incidence was slightly reduced at 20 min (only significant for 20% O2 cultures, Table 1). However, increasing PD150606 to 50 μM, a concentration not specific for calpains (Wang et al. 1996;Van den et al. 2002), resulted in significant protection of neurons at both 20 and 60 min (Table 1).
To determine whether calpain inhibition was a plausable mechanism for its protective action, we assessed the ability of PD150606 to inhibit α-spectrin breakdown. In contrast to the nearly complete inhibition of α-spectrin cleavage by calpeptin, PD150606 even at 50 μM attenuated α-spectrin breakdown by only ~35% (n=3; Fig. 5). This modest inhibition of calpain activity was unlikely to account for the potent protection of 50 μM PD150606 against DCD since the >90% inhibition of calpain proteolysis by calpeptin was without effect.
Although calpain activity was not required for DCD or recovery from RCE in response to 100 μM glutamate, it remained possible that the late calpain activation occurring ~40 min after DCD onset was necessary for the efficient execution of cell death. To address this possibility, we measured the number of healthy, apoptotic, and necrotic neurons at 4, 8, 12, or 20 hours after a 30 min excitotoxic glutamate stimulus with or without calpeptin pre-treatment (Fig. 6). PD150606 was not evaluated in this assay due to its limited efficacy as a calpain inhibitor (Fig. 5) and its ability to inhibit multiple calcium-dependent pathways (e.g. Ca2+-calmodulin dependent calcineurin activity (Wang et al. 1996)). Healthy neurons excluded the apoptosis marker Yo-Pro-1 and the necrosis marker propidium iodide (PI) from the nucleus and displayed mitochondrial (cytoplasmic) TMRM+ fluorescence. Apoptotic neurons were defined as cells with nuclear Yo-Pro-1 fluorescence that excluded PI. Neurons exhibiting nuclear PI fluorescence without cytoplasmic TMRM+ fluorescence were considered necrotic.
We observed no significant effect of calpeptin on the number of healthy or necrotic neurons at any of the time points measured, as assessed by logistic regression analysis (Fig. 6). Apoptotic neurons were not observed at any time point. We also observed no significant effect of calpeptin in parallel assays using cortical neurons cultured at 20% O2 (data not shown). Thus, cell death in response to 100 μM glutamate occurred with a normal time course in the presence of effective calpain inhibition.
Finally, although we were unable to detect a significant decrease of full-length NCX3 protein in cortical neurons exposed to glutamate (Fig. 3), it remained possible that a functional loss of NCX activity influenced DCD onset. To assess the workload on the NCX in glutamate-treated cortical neurons, the initial, glutamate-evoked rise of [Ca2+]c and [Na+c depolarization of the plasma membrane potential (ΔΨP) were measured using wide-field fluorescence microscopy. To resolve the initial effects and reversibility of the glutamate treatment, a perfusion system was used to deliver glutamate (100 μM) plus glycine (10 μM) and to wash out Mg2+.
Fig. 7a-c shows the transients of [Ca2+]c, [Na+c and ΔΨP evoked by a 160 s glutamate exposure. Remarkably, the almost instantaneous rise of [Ca2+]c (a) and depolarization of the plasma membrane to +10 mV (c) was paralleled by a gradual, high amplitude rise of [Na+] (b). The large changes in the [Na+]c and ΔΨP suggested that a forward mode of Na+-Ca2+ exchange was not feasible under these circumstances. To quantify the thermodynamic driving force on the NCX, we calculated the ΔG of the forward reaction (Ca2+ extrusion). The data in Fig. 7d indicate that after addition of glutamate the NCX operated in forward mode for only a very short initial period, during the initial rise of [Ca2+]c and before the gross rise of [Na+]c. From this time point (<10 sec after glutamate application) the NCX reversed, favoring Ca2+ entry, and remained reversed for the entire duration of the experiment (ΔG>0). While washing out glutamate led to quick re- (and hyper-) polarization of the plasma membrane and close-to-baseline recovery of [Ca2+]c (Fig. 7a and c), the elevated [Na+]c (Fig. 7b) supported a maintained reversal of the NCX (Fig. 7d). [Ca2+]c did not recover completely to baseline until the end of the experiment likely due to Ca2+ influx via the maintained reversal of the NCX (Fig. 7a).
To confirm that the NCX works in reverse during the glutamate-triggered Ca2+ plateau, the reverse mode-selective NCX inhibitor KB-R7943 (10 μM) was added on top of the glutamate treatment (Fig. 7e). A decrease in [Ca2+]c was observed upon addition of KB-R7943. This decrease suggested that Ca2+ entry through reversed NCX contributed to the glutamate-triggered Ca2+ plateau. The Ca2+ channel blocker nifedipine (1 μM) was present in this experiment to exclude the possibility that the effect of KB-R7943 was due to the inhibition of voltage-dependent Ca2+ channels (Dietz et al. 2007). Remarkably, blocking NCX activity with KB-R7943 decreased the incidence of DCD during a 20 or 60 min glutamate exposure (Table 1). Thus any inhibition of plasmalemmal NCX function by calpain or other mechanisms would delay rather than promote the induction of DCD in cortical neurons.
Calpain proteases play an undisputed but incompletely characterized role in neuronal injury. Calpain inhibitors are protective to varying degrees in animal models of stroke (Bartus et al. 1994;Markgraf et al. 1998;Tsubokawa et al. 2006), traumatic brain injury (Saatman et al. 1996;Buki et al. 2003;Ai et al. 2007), Alzheimer's disease (Trinchese et al. 2008), and Parkinson's disease (Crocker et al. 2003). Additionally, calpain-generated α-spectrin breakdown products in cerebrospinal fluid are associated with traumatic brain injury severity in humans (Pineda et al. 2007). However, despite the early promise of calpain inhibitors, data on long-term outcome in animal studies remains sparse and successful clinical studies in humans have yet to materialize. It is clear that multiple cell death mechanisms contribute to the spatiotemporal profile of neuronal loss in vivo and a better understanding of the participation of calpain proteases in the pathogenesis of different injury paradigms is required for the development of targeted treatments. In this study we set out to: 1) identify whether the major activation of calpain proteases in the cytoplasm occurs up or downstream of glutamate-induced intracellular calcium deregulation and 2) determine whether calpain activity is required for excitotoxic calcium deregulation or cell death in cortical neurons.
Using a genetically encoded calpain sensor, we demonstrated that somal cytoplasmic calpain activity becomes detectable via fluorescent substrate within a very narrowly defined time window at approximately 40 min after the onset of DCD (Fig. 1). Once initiated, cleavage of the FRET sensor progressed at a constant rate until the loss of FRET was complete (Fig. 1c), suggesting that this event represented `major' unchecked calpain activation.
The mechanisms of calpain activation in intact neurons are incompletely understood. In vitro, both μ-calpain (3-50 μM) and m-calpain (0.4-0.8 mM) require supra-physiological calcium concentrations for activation (Goll et al. 2003). Furthermore, the constitutive expression of an endogenous inhibitor protein, calpastatin, is predicted to limit calpain activity in response to transient pulses of high calcium while ultimately succumbing to proteolysis only if elevated calcium is sustained (Goll et al. 2003;Rami 2003). Thus, unchecked calpain activation in neurons is expected only after a period of prolonged calcium deregulation. Our data in Fig. 1 are consistent with a model where the calpastatin-mediated check on calpain activity is relieved following 40 min of sustained DCD, allowing unimpeded calpain proteolysis of cytoplasmic substrates to progress. Because calpain is reportedly sequestered in subcellular membrane fractions including mitochondria (Hewitt et al. 1998;Garcia et al. 2005;Ozaki et al. 2007;Kar et al. 2008), an alternative possibility is that the delayed but distinct cytoplasmic calpain activation with respect to DCD represents a subcellular translocation event that brings active calpain in contact with the fluorescent sensor.
Spectrin breakdown products and a decrease in FRET were reported in pYSCS-transfected hippocampal neuron dendritic spines within 5 min of glutamate treatment (Vanderklish et al. 2000). In our experiments, glutamate treatment of cortical neurons caused an initial calpain-independent, reversible decrease in FRET, suggesting that conformational changes of the probe affect FRET efficiency. While the spectral unmixing and three-wavelength FRET calculation avoided errors originating from spectral bleed-through and variations of FRET acceptor fluorescence yield, these calculations also decreased signal-to-noise ratio. Due to the high calpain-insensitive background we cannot confirm or reject the possibility of localized calpain activation (e.g. in sub-plasma membrane regions) prior to DCD. However, our data do not support a generalized early increase in calpain activity.
The literature on whether calpain activation causatively contributes to glutamate receptor-mediated cell death is inconsistent. Early studies found that calpain activity was not required for glutamate excitotoxicity in cerebellar granule (Manev et al. 1991) or hippocampal (Adamec et al. 1998) neurons, while neuroprotection by calpain inhibitors was reported by others in response to glutamate, kainate, or NMDA (Brorson et al. 1995;Rami et al. 1997;Lankiewicz et al. 2000;Tremblay et al. 2000;Araujo et al. 2004;Bano et al. 2005;Hou et al. 2006;Araujo et al. 2007;Beart et al. 2007;Bevers et al. 2009). Although few of these studies addressed the mechanistic involvement of calpains in death, one found that calpain inhibition delayed the late stages of AMPA-induced cortical neuron death (PI positivity) without preventing early hallmarks of mitochondrial dysfunction (decreased dehydrogenase activity, cytochrome c release) (Beart et al. 2007). However, another found that overexpression of calpastatin inhibited DCD in cerebellar granule neurons (Bano et al. 2005), suggesting an upstream role for calpain involvement in excitotoxic cell death.
Here we found that calpeptin effectively inhibited calpain-mediated α-spectrin breakdown (Fig 3) without decreasing the incidence or onset of glutamate-induced DCD (Fig. 2, Table 1). In fact, a small but significant increase in the incidence of DCD was detected when large numbers of neurons were considered, possibly due to inhibition of local calpain processing of NMDA NR2 subunits (Wu et al. 2005). Calpeptin was also unable to improve the rate of sustained [Ca2+]c recovery following glutamate-triggered DCD-like reversible calcium elevations (Fig. 4 and Supplemental Movie 3), or protect against necrotic cell death (Fig. 6). Apoptotic death was not observed in the present study. Because calpain inhibition protects against neuronal apoptosis (Ray et al. 2006;Cao et al. 2007), we hypothesize that the percentage of neurons undergoing apoptosis vs. necrosis in response to a given excitotoxic challenge ultimately determines the ability of calpain inhibitors to effectively delay glutamate receptor-related injury.
Our primary findings contrast with a recent report that overexpression of calpastatin inhibits DCD in cerebellar granule neurons by preventing calpain-mediated NCX3 cleavage (Bano et al. 2005). The onset of DCD in cerebellar granule neurons is considerably delayed relative to cortical neurons (e.g. compare Fig. 2 to (Bano et al. 2005)) and it is possible that different mechanisms (apoptotic vs. necrotic) contribute to injury. The expression of NCX3 relative to NCX1 also differs between cortical and cerebellar granule neurons (Kiedrowski et al. 2004). We failed to see the appearance of 58-60 kD NCX3 breakdown products or a degradation of full-length NCX3 in glutamate-treated cortical neurons even after 1.5 hr of continuous glutamate treatment (and ~1 hr after DCD, Fig. 3). The NCX antagonist KB-R7943 delayed rather than shortened the time to DCD (Table 1), suggesting that in our model NCX activity following glutamate exposure was deleterious rather than protective. Calculation of the ΔG for Na+/Ca2+ exchange (Fig. 7) revealed that NCX operates in reverse mode favoring Ca2+ entry during glutamate exposure, in agreement with previous reports (Kiedrowski et al. 1994;Hoyt et al. 1998;Czyz and Kiedrowski 2002;Kiedrowski et al. 2004;Araujo et al. 2007;Storozhevykh et al. 2007). Consistent with reverse mode NCX operation, KB-R7943 acutely decreased intracellular Ca2+ when administered after glutamate addition (Fig. 7e). Thus, NCX failure cannot explain the sudden loss of calcium homeostasis in glutamate-exposed cortical neurons although failure of other calcium extrusion pathways (e.g. the PMCA (Pottorf et al. 2006)) may contribute.
In summary, our studies for the first time imaged somal calpain activation relative to intracellular calcium changes in live glutamate-treated neurons, revealing the onset of major activity following ~40 min of sustained DCD. Modifications of this technique, particularly ones that reduce pH-sensitive and calpeptin-insensitive changes in fluorescence, should be useful for characterizing calpain activation in less acute models of neuronal injury where calpain inhibitors are protective.
We thank Richard Oh for the preparation of primary cortical and hippocampal neurons, Dr. Ken Philipson for generously providing NCX3 antibody, and Dr. Peter Vanderklish for kindly providing the pYSCS plasmid. This work was supported by NIH grants PL1 AG032118 and P30 AG025708 at the Buck Institute and by NIH grant NS054764 to B.M.P.