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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Nature. Author manuscript; available in PMC 2009 September 15.
Published in final edited form as:
PMCID: PMC2744041
NIHMSID: NIHMS103020

Runx1 is required for the endothelial to hematopoietic cell transition but not thereafter

Abstract

HSCs are the founder cells of the adult hematopoietic system, and thus knowledge of the molecular program directing their generation during development is important for regenerative hematopoietic strategies. Runx1 is a pivotal transcription factor required for HSC generation in the vascular regions of the mouse conceptus - the aorta, vitelline and umbilical arteries, yolk sac and placenta 1, 2. It is thought that HSCs emerge from vascular endothelial cells through the formation of intra-arterial clusters 3 and that Runx1 functions during the transition from ‘hemogenic endothelium’ to HSCs 4, 5. Here we show by conditional deletion that Runx1 activity in vascular endothelial cadherin (VEC) positive endothelial cells is indeed essential for intra-arterial cluster, hematopoietic progenitor, and HSC formation. In contrast, Runx1 is not required in cells expressing Vav, one of the first pan-hematopoietic genes expressed in HSCs. Collectively these data show that Runx1 function is essential in endothelial cells for hematopoietic progenitor and HSC formation from the vasculature, but its requirement ends once or before Vav is expressed.

HSCs are found in several independent sites in the conceptus, and the transcription factor Runx1, which is required for HSC generation, is expressed in these sites 4. Runx1 protein is seen in the mesodermal masses and endoderm of the prospective yolk sac blood islands at the neural plate stage 4, 6, in mesoderm of the distal allantois and chorion (precursors of the placenta) at headfold stages 7, and in endothelial cells in the distal allantois, dorsal aorta, vitelline and umbilical arteries starting the 4–6 somite pair (s) stage (data not shown). Runx1 is expressed at 9.5–10.5 days post coitus (dpc) in both endothelial and mesenchymal cells in the dorsal aorta and placental labyrinth 4, 7, 8. Therefore it is not clear whether Runx1 is required in mesoderm, endoderm, mesenchyme, and/or endothelial cells for intra-arterial cluster and HSC formation.

To examine the hypothesis that Runx1 is required in endothelial cells per se we deleted Runx1 in VEC positive cells. VEC is a transmembrane protein located at cell-to-cell adherens junctions that is involved in endothelial cell adhesion 9. VEC is expressed transiently in the yolk sac mesoderm at 7.5 dpc, however at 8.5 dpc its expression is highly restricted to endothelial cells in the dorsal aorta and heart 1012. At 9.5 dpc VEC is expressed in vasculature throughout the conceptus 11. Distinct mesodermal populations give rise to the yolk sac blood islands and to the major arteries of the conceptus, including those in the placenta 1315. Thus with VEC-Cre we could monitor the impact of Runx1 excision on HSC formation from the vasculature including the dorsal aorta, vitelline and umbilical arteries, and placenta - sites where VEC expression initiates in endothelium.

We generated a VEC-Cre transgene (Fig. 1a) and used R26R-lacZ and R26R-YFP reporter mice to characterize its activity. The first sites of VEC-Cre activity were in the yolk sac and chorionic mesoderm (not shown). Primitive erythrocytes in the yolk sac blood islands were β-gal+, as were PECAM1+ cells in the vitelline vasculature (Fig. 1b). By 9.5 dpc β-gal activity was high in vasculature throughout the conceptus (Fig. 1c), particularly within PECAM1+ cells in the developing labyrinth of the placenta and in the umbilical artery (Fig. 1d). At 10.5 dpc β-gal+ endothelial cells and intra-arterial clusters were present in the dorsal aorta, vitelline and umbilical arteries, whereas mesenchymal cells were β-gal (Fig. 1f). VEC-Cre mediated excision occurred in 77% (± 11%) of cell surface Sca1 VEC+ endothelial cells in the AGM region plus vitelline and umbilical arteries (AGM+V+U) of 11.5 dpc conceptuses and in 86% of Sca1+ VEC+ cells (Fig. 1e) (Sca-1 marks 50% of HSCs, and VEC marks all HSCs in the AGM region) 2, 16. No excision was detectable in Sca1+ VEC cells (Fig. 1e). There was widespread VEC-Cre activity in the fetal liver (Fig. 1f,g), 99% of which was localized to CD45+, CD71+, and/or Ter119+ blood cells in R26R-YFP;VEC-Cre conceptuses (not shown). Approximately 85% of fetal liver blood cells in 15.5 dpc R26R-YFP;VEC-Cre fetuses were YFP+ (Fig, 1h), and are consequently derived from cells that at one time in their history expressed VEC. Ninety six percent of CD45+ adult bone marrow cells were YFP+ (Fig. 1i), and thus almost all adult blood is derived from VEC-expressing cells.

Figure 1
VEC-Cre excision marks endothelium and blood

VEC-Cre mediated Runx1 deletion caused a 65% rate of fetal lethality (Supplementary Table 1), and approximately 10% of 12.5 dpc Runx1f/f; VEC-cre fetuses had central nervous system hemorrhaging and fetal liver anemia characteristic of Runx1 deficiency 17, 18 (Fig. 2a and Supplementary Table 1). There was an 8-fold decrease in c-kit+ cells in the livers of 11.5 dpc Runx1f/f; VEC-cre fetuses (Fig. 2b), and in colony-forming unit culture (CFU-C) progenitors in all hematopoietic sites (Fig. 2c). DNA analysis revealed that 84% of colonies derived from Runx1f/+;VEC-Cre fetal cells had excised the Runx1f allele (Fig. 2d and Supplementary Table 2), consistent with the deletion efficiency determined with the R26R-YFP allele. In contrast, only one out of 389 colonies picked from cultures established from Runx1f/f;VEC-cre fetuses had two deleted Runx1f alleles. Thus, at least one functional Runx1 allele must be present in a VEC+ cell for CFU-C progenitors to emerge in the fetus.

Figure 2
Runx1 is required in VEC+ cells for hematopoietic progenitor formation

In vivo repopulation assays revealed that hematopoietic tissues of 11.5 dpc Runx1f/f;VEC-Cre conceptuses could engraft adult mice, but at a much reduced frequency as compared to Runx1f/+;VEC-cre cells (Fig. 3a,b). While all donor-derived CFU-C progenitors in the recipients of Runx1f/+;VEC-Cre HSCs had a deleted Runx1f allele, both Runx1f alleles were deleted in only one out of 192 colonies generated from Runx1f/f;VEC-Cre donor-derived HSCs (Fig. 3c). We also analyzed the bone marrow of viable Runx1f/f;VEC-Cre adult mice and found that all colonies retained at least one undeleted Runx1f allele (Supplementary Table 2). Therefore there is strong selective pressure for cells that escape the excision of at least one Runx1f allele to contribute to hematopoiesis in Runx1f/f;VEC-Cre mice. Together these data indicate that Runx1 is required in VEC+ cells for HSC formation.

Figure 3
Runx1 is required in VEC+ cells for HSC emergence

As the autonomous production of HSCs occurs first in the major arteries of the conceptus at 10.5 dpc (dorsal aorta, vitelline and umbilical) 19, and HSCs localize to the walls of these arteries 2, 16, we examined intra-arterial cluster formation in Runx1f/f;VEC-Cre embryos. VEC is expressed on both endothelial cells and intra-arterial clusters, but the first potential site of deletion is in the endothelium that gives rise to the clusters. No hematopoietic clusters were visible in the dorsal aorta, vitelline and umbilical arteries of Runx1f/f;VEC-Cre animals, and there was a 90-fold decrease in c-kithi cells (Fig. 3d).Thus, the important deletion event in these arteries occurred in the endothelium.

To confirm this and to determine whether Runx1 continues to be required once the endothelial to HSC transition is complete, we examined the outcome of deleting Runx1 with Vav-Cre. Vav1 is a GDP/GTP nucleotide exchange factor for Rho/Rac, whose expression is restricted to hematopoietic cells 20, 21. Vav1 was also implicated as a Runx1 target, as its transcript was not detected in the AGM region or fetal liver of Runx1 deficient embryos 22. Excision of the R26R-YFP allele by Vav-Cre occurred in 68% (±16%) of CD45+VEC hematopoietic cells from the AGM region, vitelline and umbilical arteries and not in CD45VEC+ endothelium (Fig. 4a). Excised (β-gal+) cells were not found in intra-arterial clusters at either 10.5 or 11.5 dpc, and were instead located within the sub-aortic mesenchyme and the circulation (Fig. 4b–d). Therefore Vav-Cre marked cells that either originated in the yolk sac, or had upregulated Vav-Cre subsequent to their release from the intra-arterial clusters. β-gal+ cells were detectable in the fetal liver at 10.5 dpc (not shown), and by 11.5 dpc 63% (±13%) of CD45+VEC cells in the fetal liver were YFP+ (Fig. 4a) and were easily seen by histology (Fig. 4e). Fetal liver HSCs transiently express cell surface VEC 23, 24. Sixty three (±15) percent of the CD45+VEC+ HSC-containing population in the 11.5 dpc fetal liver was YFP+ (Fig. 4a), and by 15.5 dpc 90% of lineage negative Sca1+ Mac1+ fetal liver cells were YFP+ (not shown), similar to the excision frequencies reported previously 21. Thus with Vav-Cre we could execute an excision in CD45+ cells while avoiding deletion in the intra-arterial clusters or endothelium.

Figure 4
Runx1 is not required in Vav+ cells for CFU-C or HSC emergence

In contrast to VEC-Cre, excision of Runx1 with Vav-Cre did not affect fetal or adult viability (Supplementary Table 3). The hematopoietic deficiencies described in adult mice upon Runx1 deletion with Mx1-Cre, including thrombocytopenia, defective lymphopoiesis, and expansion of the lineage negative (Lin) Sca-1+ c-kit+ population 25, 26 were also apparent in the Vav-Cre deleted mice (Supplementary Figure 2). Fetal livers from 15.5 dpc Runx1f/f;Vav-Cre mice had increased numbers of CFU-C progenitors, particularly granulocyte/macrophage (GM) and multipotent (GEMM) progenitors (Fig. 4f), as was previously observed in the bone marrow of Mx1-Cre deleted adult mice 25, 26. Thus deletion of Runx1 in Vav+ cells did not impair the emergence of CFU-C progenitors but did affect their homeostasis. Importantly, both Runx1f alleles were deleted in 280/281 colonies from 15.5 dpc Runx1f/f;Vav-Cre fetal livers (Supplementary Table 4). Colonies from 11.5 dpc animals contained Runx1f alleles at various stages of deletion (Fig. 4g) since excision is initiating close to that time. However, 32% of colonies from the hematopoietic tissues of 11.5 dpc fetuses contained two deleted Runx1f alleles, and by 14.5 dpc 100% of fetal liver progenitors had deleted both alleles (Supplementary Table 4). We therefore conclude that Runx1 function is no longer required once a CFU-C progenitor expresses Vav.

Mice were efficiently engrafted with hematopoietic tissues from 11.5 dpc Runx1f/f;Vav-Cre conceptuses (Fig. 4h), and in all colonies (94/94) derived from donor Runx1f/f;Vav-Cre HSCs, both Runx1f alleles were deleted (Supplementary Table 4). The same was true in adult Runx1f/f;Vav-Cre mice – virtually all CFU-C progenitors had deleted both Runx1f alleles (Supplementary Table 4). Hence, Runx1 is not required in a Vav+ HSC.

In summary, the most essential function of Runx1 is during the transition from VEC+ cells to Vav+ HSCs, although it continues to provide important activity in maintaining normal HSC function and for the terminal differentiation of some lineages 25, 26 (Supplementary Figure 1). Our data demonstrate that the relevant VEC+ HSC precursors are in the hemogenic endothelium. Although the yolk sac mesoderm expresses VEC, in the placenta, vitelline and umbilical arteries, and dorsal aorta, which are independent hematopoietic sites derived from distinct populations of mesoderm 1315, VEC expression is restricted to intra-arterial clusters and endothelium. Intra-arterial clusters in these sites are absent in Runx1f/f;VEC-Cre conceptuses, and therefore the relevant deletion event must have occurred in the endothelium from which they are derived. Continued deletion by VEC-Cre in fetal liver HSCs is unlikely because Runx1 is no longer required once HSCs reach the fetal liver and express Vav, yet they all contain undeleted Runx1f alleles.

We also showed that most adult blood (≥ 95%) is derived from a cell that at one time in its history expressed VEC. Our working model is that most HSCs are born from VEC+ hemogenic endothelium in the conceptus, although a VEC+ HSC precursor in adult bone marrow cannot be ruled out. Monvoisin et al. 27 found that a small but detectable number of bone marrow cells (< 0.4%) could be labeled when a tamoxifen-regulated VEC-Cre was activated in the adult, hence VEC+ adult HSC precursors, if they exist, are rare cells.

Another model for HSC emergence posits that HSCs in the AGM region originate in subaortic patches of cells (SAPs) in the ventral para-aortic mesenchyme of the dorsal aorta 28, and migrate toward the aorta and between endothelial cells to form the intra-aortic clusters. As we showed that almost all blood is derived from VEC+ cells, SAP cells must upregulate VEC as they protrude through the endothelium.

Methods

Conditional deletion by VEC-Cre or Vav-Cre

A 2.5 kb VEC promoter sequence was cloned between insulators from the chicken γ-globin gene 29 and used to drive Cre expression. The construct was injected into fertilized oocytes from C57BL6/J x 129S1/SvImJ F1 mice. R26R-lacZ/+;VEC-Cre or R26R-YFP/+;VEC-Cre conceptuses were generated by crossing VEC-Cre/+ males with R26R-lacZ/+ or R26R-YFP/+ females. Runx1f/+;VEC-Cre and Runx1f/f;VEC-Cre conceptuses were generated by crossing Runx1f/+; VEC-Cre/+ males with Runx1f/f females. Runx1f/+;R26R-YFP/+;VEC-Cre or Runx1f/f;R26R-YFP/+;VEC-Cre conceptuses were generated from crossing Runx1f/+;R26R-YFP/+; VEC-Cre/+ males with Runx1f/f females. Similar breeding strategies were employed for Vav-Cre deletion.

Microscopic and histological analyses

Conceptuses were suspended in phosphate-buffered saline or in a 1:2 mixture of benzyl alcohol/benzyl benzoate and visualized with a stereomicroscope. X-gal (Sigma) staining was performed as described previously 4. In some cases the conceptuses were also incubated with rat anti-mouse Pecam1 and ABC reagent at 4°C 30, then treated with DAB peroxidase substrate. Conceptuses were embedded in paraffin, sectioned, and counterstained with nuclear fast red.

To analyze intra-aortic clusters, embryos were fixed and stained with anti-c-Kit and anti-PECAM1-antibodies. A 1:2 mix of benzyl alcohol/benzyl benzoate was used to increase the transparency of tissues. Samples were analyzed with a confocal microscope using the multi track sequential mode. Three-dimensional reconstructions were generated from Z-stacks (62–87 serial sections).

Hematopoietic assays

Methylcellulose colony forming assays were performed as described previously 4 and colonies counted after 7 days. Fetal tissues were transplanted as previously described 16.

Immuno-staining was performed with phycoerytherin, allophycocyanin, or Alexa Fluor 647 conjugated antibodies Stained cells were analyzed on a Becton Dickinson FACSCalibur flow cytometer. Dead cells were excluded with 7AAD.

Supplementary Material

1

Supplementary Information is linked to the online version of the paper at www.nature.com/nature.

Acknowledgments

The authors thank Gary Ward for his assistance with flow, Thomas Graf for the Vav-Cre mice, and Philippe Huber for the VEC regulatory sequences. This work was supported by R01HL091724 (NAS), R01DK54077 (ED), and T32 AI-07519 (BMZ). Core services were supported in part by the Norris Cotton Cancer Center (NIH CA23108) and the Abramson Family Cancer Research Institute.

Tomomasa Yokomizo performed the experiments in Figure 3d.

Brandon Zeigler performed the experiments in Figures 1b and 1d.

Michael Chen performed all the remaining experiments.

Elaine Dzierzak participated in the interpretation of the experiments and writing the manuscript.

Nancy A. Speck participated in the design and interpretation of the experiments, wrote the manuscript, and made the figures.

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